CN105746584B - A kind of Pesticidal combination and its preparation method and application containing Metarhizium anisopliae and cyromazine - Google Patents
A kind of Pesticidal combination and its preparation method and application containing Metarhizium anisopliae and cyromazine Download PDFInfo
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- CN105746584B CN105746584B CN201610196602.8A CN201610196602A CN105746584B CN 105746584 B CN105746584 B CN 105746584B CN 201610196602 A CN201610196602 A CN 201610196602A CN 105746584 B CN105746584 B CN 105746584B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/66—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms
- A01N43/68—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms with two or three nitrogen atoms directly attached to ring carbon atoms
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Abstract
The invention discloses a kind of Pesticidal combination and its preparation method and application containing Metarhizium anisopliae and cyromazine, the Pesticidal combination is using Metarhizium anisopliae (Metarhizium anisopliae) and cyromazine as primary activity ingredient, in following ratio mixing Metarhizium anisopliae conidium (a)：Cyromazine (gram)=(49 1) × 2 × 1010：(1‑49).Active constituent is added adjuvant and can be made into wet powder, water dispersible granules and oil-suspending agent, it can be used for preventing the pests such as scale insect, trialeurodes vaporariorum, thrips, grub, wireworm, citrus fruit fly, liriomyza bryoniae, root maggot, Bradysia odoriphaga, it is particularly suitable for the prevention of the Diptera pests such as citrus fruit fly, liriomyza bryoniae, root maggot, bradysia odoriphaga, has many advantages, such as that efficacy stability, quick-acting are good, the lasting period is long and safety and environmental protection.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of insecticide composition containing Metarhizium anisopliae and cyromazine
Object and its preparation method and application.
Background technology
Metarhizium anisopliae (Metarhizium anisopliae) is a kind of insect pathogenic fungus of wide spectrum, has distribution
The features such as range is big, parasitic wide spectrum, to people, animal and crop nonhazardous, can parasitic various insects, be to study and answer on our times
With most one of entomogenous fungis.According to incompletely statistics, more than 200 kinds of whole world Diptera, Lepidoptera, coleoptera, Homoptera etc.
Insect can be lethal by this fungal infection.Green muscardine fungus has significant insecticidal effect, and with different from existing insecticide
The completely new mechanism of action mainly acts on insect hemolymph immune system, is allowed to lose immune defense function.The whole world is to green muscardine fungus
Research have more than 100 years history, currently, domestic existing Metarhizium anisopliae bacterial strain exploitation is mainly used for preventing at insecticide
Control beach/grassland grasshopper, Chinese cabbage beet armyworm, apple tree small heart-eating peach worm.But due to being restricted by many factors, cause
The practical preventive effect in its crop field is unstable, greatlys restrict its large-scale production and application.Develop Metarhizium anisopliae compound insecticidal
Agent, to prevention subterranean pest-insect and Fruit fly, has important there is an urgent need for overcoming its control effect unstable and the defect of slowly effect
Meaning.
Cyromazine belongs to 1,3,5-triazines class insect growth regulator, IGR, has sp act to Diptera larvae, there is strong interior suction
Conduction.Lure that Diptera larvae and pupa are morphologically distorted into, adult eclosion is not complete or suppressed, can be used for preventing spot
The pests such as latent fly, citrus fruit fly, root maggot, Bradysia odoriphaga have preferable activity to larva and pupa, are imitated to the prevention of adult
Fruit is poor, long-term to be used alone, and pest can be caused to develop immunity to drugs.
Leek root maggot scientific name is Bradysia fungus gnat Bradysia odoriphaga Yang et Zhang
Endanger the primary pest of China Specialty vegetable leek.Bradysia odoriphaga cluster endangers leek underground rhizome, causes blade to rot, leek is withered
Extremely, leek yield and quality is seriously affected.At present chemical pesticide such as acephatemet, parathion, chlopyrifos, phoxim are commonly used in production
Pellet etc. carries out pouring root prevention, or even some application high-toxic pesticide thimets are prevented, and lead to excessive pesticide residues in leek, China was once
Through caused by " the malicious leek event " in Shandong and Henan appearance is exactly to prevent bradysia odoriphaga with highly toxic pesticide, thus danger coerces consumer's
Health, foreign exchange earning also receive prodigious influence.
It is the important method for overcoming or delaying drug resistance using pesticide combination technology or mixing application technique, influences elder brother
The moulting hormone of worm reduces the weight of insect, inhibits its locomitivity, is conducive to raising Metarhizium anisopliae and is infected to pest
Rate improves insecticide efficiency.At present it is not yet found that the compounded pesticides of relevant Metarhizium anisopliae and cyromazine.
Invention content
The purpose of the present invention is to overcome the deficiencies of prior art and provide a kind of Pesticidal combinations, the Pesticidal combination is pollution-free,
Low-residual, safety and environmental protection all have preferable control effect to a variety of worm states.
The present invention additionally provides the preparation and application of the Pesticidal combination simultaneously.
The invention is realized by the following technical scheme：
A kind of Pesticidal combination of the present invention, contains Metarhizium anisopliae (Metarhizium anisopliae) and fly eradication
Amine, the Metarhizium anisopliae and the cyromazine are mixed in following ratio：The conidial number of Metarhizium anisopliae
Grams ratio with the cyromazine is (49-1) × 2 × 1010：(1-49).
Preferably, the composition is made of Metarhizium anisopliae, cyromazine and adjuvant.
For the present invention using Metarhizium anisopliae and cyromazine as host agent, addition adjuvant can be processed into wettable powder, water-dispersed
The preparations such as granula, oil-suspending agent.The adjuvant changes because of the dosage form of processing difference.
Preferably, the adjuvant is wetting agent, dispersant, stabilizer and carrier, and the weight of each component of the adjuvant accounts for
The percentage of the total weight of the Pesticidal combination is respectively：Wetting agent accounts for 5-35%, and dispersant accounts for 5-30%, and stabilizer accounts for
0%-5%, carrier complement to 100%；The wetting agent is TERWET1004, neopelex, alkylnaphthalene sulfonate
With one kind in anion humectant mixture (MorwetEFW), tea seed cake, spaonin powder, spaonin powder, silkworm excrement and pull open powder or more
Kind；The dispersant is lignosulfonates, modified lignin mahogany sulfonate, naphthalene sulfonic acid-formaldehyde condensation product, alkyl naphthalene formaldehyde condensation
Object sulfonate, fatty alcohol polyoxyethylene ether, nekal and lignosulfite it is one or more；The stabilization
Agent is one in butylated hydroxyarisol, 2,6-di-tert-butyl p-cresol, propylgallate and tert-butylhydroquinone
Kind is a variety of；The carrier is one or more in kaolin, white carbon, attapulgite, diatomite and bentonite.
Above-mentioned adjuvant is processed into wettable powder, cyromazine, wetting agent, dispersant, carrier, stabilizer is mixed, pre-
It is uniformly mixed in mixed tank, superfine powder is made after being smashed by air jet mill, is uniformly mixed with Metarhizium anisopliae conidia powder.
Preferably, the adjuvant is wetting agent, dispersant, disintegrant, binder, stabilizer and carrier；The adjuvant
The percentage that the weight of each component accounts for the total weight of the Pesticidal combination is respectively：Wetting agent accounts for 1-15%, and dispersant accounts for 2-
20%, disintegrant accounts for 1-18%, and binder accounts for 1-5%, and stabilizer accounts for 0%-5%, and carrier complements to 100%；The wetting agent
For polyether-modified polysiloxane, lauryl sodium sulfate, fatty acid sulfates, fatty alcohol polyoxyethylene ether, alkyl phenolic group
It is one or more in polyoxyethylene ether；The dispersant is lignosulfonates, modified lignin mahogany sulfonate, naphthalene sulfonic acids first
Aldehyde condensate, alkyl naphthalene formaldehyde condensate sulfonate, fatty alcohol polyoxyethylene ether, nekal and sulfite cellulose are useless
It is one or more in liquid；The disintegrant is sodium bicarbonate, bentonite, ammonium sulfate, urea, magnesium sulfate, sodium carbonate and chlorination
It is one or more in aluminum sucrose；The binder be paraffin oil, polyethylene glycol, polyvinyl alcohol, cyclodextrin, starch, sodium metasilicate,
It is one or more in phenolic resin and methylcellulose；The stabilizer is butylated hydroxyarisol, 2,6-, bis- tertiary fourths
It is one or more in base paracresol, propylgallate and tert-butylhydroquinone；The carrier be kaolin, white carbon,
It is one or more in attapulgite, diatomite and bentonite.
Above-mentioned adjuvant is processed into water dispersible granules, weigh the high cryptogam of Metarhizium anisopliae, cyromazine, wetting agent, dispersant,
After disintegrant, stabilizer, carrier proportionally mix coarse crushing, is crushed with airslide disintegrating mill and superfine powder is made, gained is ultra-fine
Powder hybrid adhesive in fluidized bed pelletizer is granulated, and carries out sieving to obtain product through vibrating screen after dry.
Preferably, the adjuvant be wetting agent, emulsifier, stabilizer and oil base, the adjuvant be wetting agent, emulsifier,
Stabilizer and oil base, the percentage that each component of the adjuvant accounts for the total weight of the Pesticidal combination are respectively：Wetting agent accounts for
1-15%, emulsifier account for 1-15%, and stabilizer accounts for 0%-5%, and oil base supplies 100%；The wetting agent is polyether-modified poly- to have
In organic siloxane, lauryl sodium sulfate, fatty acid sulfates, fatty alcohol polyoxyethylene ether and alkyl phenolic group polyoxyethylene ether
It is one or more；The emulsifier is sorbitan monooleate, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol
With in ethylene oxide condensate, polyoxyethylene fatty acid ester, triethanolamine list olein and castor oil and ethylene oxide condensate
One or more；The stabilizer is dibutyl hydroxy toluene, 2,6-di-tert-butyl p-cresol, propylgallate and Te Ding
It is one or more in base hydroquinone；The oil base is rapeseed oil, sesame oil, peanut oil, cottonseed oil, salad oil and paraffin oil
In it is one or more.
Above-mentioned adjuvant is processed into oil-suspending agent, by cyromazine, Ricinate, emulsifier, stabilizer, oil base by formula
Ratio sequentially adds in mixing kettle, stirs evenly, and is sanded, is sanded extremely into sand mill after the shearing of high shear dispersion kettle
90% grain size is uniformly mixed after being less than 5 microns with muscardine spore powder up to oil-suspending agent product.
The dosage form of the composition is wettable powder, water dispersible granules or oil-suspending agent.
Preferably, the dosage form of the composition is oil-suspending agent, the conidial number of the Metarhizium anisopliae and institute
The grams ratio for stating cyromazine is 9 × 2 × 1010：1.
Above-mentioned Pesticidal combination can be applicable to prevention scale insect, trialeurodes vaporariorum, thrips, grub, wireworm, citrus fruit fly, spot
In latent fly, root maggot, bradysia odoriphaga and Bradysia odoriphaga.
The present composition endangers various root maggots and the leek maggot of foot end particularly suitable for prevention, applicable
Place or plant include cotton, corn, potato, beans, melon, brassicaceous vegetable, spinach, green onion, garlic, leek etc..Application method
Spraying, leaching can be taken to fill, spread fertilizer over the fields, the above application method can be combined with further mixed soil.In adult eclosion peak period,
Field furrow are sprayed with the present composition, adult can be killed.It was found that when larva, ovum and children can be killed with present composition pouring root
Worm.Formulation rate is determined according to worm kind, insect density, worm age and the conditions differences such as soil, crop through simple experiment.
The compatibility of muscardine spore and cyromazine in the Pesticidal combination of the present invention is good, and there is apparent synergy to make
With compared with single dose pesticide, insecticidal toxicity significantly improves, efficacy stability, and quick-acting is good；The usage amount of chemical pesticide is reduced, ring
Border pollution is reduced；The present invention reduces the usage amount of green muscardine fungus, reduces production cost；By the entirely different pesticide of two kinds of mechanism of action
Ingredient is compounded, and target site is increased, and insect is made to be not likely to produce drug resistance；Composition after compounding to the ovum of pest, larva,
Pupa, adult have preferable control effect.
Specific implementation mode
To keep the present invention easier to understand with reference to specific embodiments the present invention is further explained.It should be understood that this
A little examples are only for illustrating the present invention and not for limiting the scope of the present invention, unmentioned specific experiment in the following example
Method is usually carried out according to routine experiment method.
Percentage in embodiment refers both to weight percent, and the muscardine spore refers both to the mitogenetic spore of Metarhizium anisopliae
The amount of son, the cyromazine is effective quantity, and reality is converted when preparing according to active compound content used, and the amount of deviation is by filling out
Material addition is adjusted.
The embodiment of the present invention is the method being combined using indoor virulence test and field trial.
Embodiment 1：Metarhizium anisopliae cyromazine wettable powder (the conidial number of Metarhizium anisopliae and institute
The grams ratio for stating cyromazine is 39 × 2 × 1010：1)
Weigh 390 grams of Metarhizium anisopliae conidia powder, 10.2 grams of cyromazine, 105 grams of wetting agent neopelex,
150 grams of spaonin powder, 50 grams of dispersant lignosulfonates, 150 grams of modified lignin mahogany sulfonate, stabilizer tertiary butyl is to hydroxyl fennel
12 grams of fragrant ether, it is 1000 grams that surplus, which is diatomite as carrier benefit ad pond om,.
The cyromazine, the wetting agent, the dispersant, the stabilizer and the carrier are mixed in premixing tank
Uniformly, superfine powder is made after being smashed by air jet mill, is uniformly mixed with Metarhizium anisopliae conidia powder, obtains wettable powder
Pesticidal combination.
In a further advantageous embodiment, Metarhizium anisopliae cyromazine wettable powder, wherein chafer are formulated as
The grams ratio of the conidial number of green muscardine fungus and the cyromazine is 49 × 2 × 1010：1.Weigh Metarhizium anisopliae conidia powder
490 grams, 10.2 grams of cyromazine, 100 grams of wetting agent TERWET1004,105 grams of dispersant naphthalene sulfonic acid-formaldehyde condensation product, alkylnaphthalene
95 grams of formaldehyde condensate sulfonate, 20 grams of stabilizer 2,6-di-tert-butyl p-cresol, surplus be attapulgite as carrier mend to
Total weight is 1000 grams.
In a further advantageous embodiment, Metarhizium anisopliae cyromazine wettable powder, wherein chafer are formulated as
The grams ratio of the conidial number of green muscardine fungus and the cyromazine is 1 × 2 × 1010：1.Weigh Metarhizium anisopliae conidia powder
10 grams, 10.2 grams of cyromazine, 105 grams of wetting agent alkylnaphthalene sulfonate and anion humectant mixture MorwetEFW, without trouble
35 grams of sub- powder, 120 grams of dispersant fatty alcohol polyoxyethylene ether, 10 grams of stabilizer tert-butylhydroquinone, tertiary butyl are to hydroxyl fennel
10 grams of fragrant ether, it is 400 grams that surplus, which is kaolin as carrier benefit ad pond om,.
In a further advantageous embodiment, Metarhizium anisopliae cyromazine wettable powder, wherein chafer are formulated as
The grams ratio of the conidial number of green muscardine fungus and the cyromazine is 1 × 2 × 1010：49.Weigh Metarhizium anisopliae conidia powder
10 grams, 499.8 grams of cyromazine, 105 grams of wetting agent silkworm excrement, 100 grams of pull open powder, 50 grams of tea seed cake, dispersant nekal
105 grams, 150 grams of lignosulfite, it is 5100 grams that surplus, which is white carbon and bentonite as carrier benefit ad pond om,.
Embodiment 2：Metarhizium anisopliae cyromazine oil-suspending agent (the conidial number of Metarhizium anisopliae with it is described
The grams ratio of cyromazine is 9 × 2 × 1010：1).
Weigh 20,000,000,000 spore of Metarhizium anisopliae/90 grams of g high cryptogams, 10.2 grams of cyromazine, emulsifier Sorbitan alcohol ester
100 grams of fat acid ester polyoxyethylene ether, 50 grams of wetting agent lauryl sodium sulfate, 30 grams of stabilizer tert-butylhydroquinone, oil base
499.8 grams of 450 grams of peanut oil and sesame oil.
The cyromazine, the wetting agent, the emulsifier, the stabilizer and the oil base are sequentially added into mixing kettle
In, it stirs evenly, is sanded, is sanded after being less than 5 microns to 90% grain size into sand mill after the shearing of high shear dispersion kettle
It is uniformly mixed with the muscardine spore powder, obtains oil-suspending agent disinfestation composition.
In another embodiment, component is formulated as 20,000,000,000 spore of Metarhizium anisopliae/90 grams of g high cryptogams, cyromazine
10.2 grams, 150 grams of emulsifier sorbitol acid anhydride list olein, 150 grams of the polyether-modified polysiloxane of wetting agent, oil base rapeseed oil
450 grams and 149.8 grams of cottonseed oil.
In another embodiment, component is formulated as 20,000,000,000 spore of Metarhizium anisopliae/90 grams of g high cryptogams, cyromazine
10.2 grams, emulsifier fatty alcohol and 5 grams of ethylene oxide condensate, 5 grams of polyoxyethylene fatty acid ester, wetting agent fatty acid sulfates
5 grams, 5 grams of fatty alcohol polyoxyethylene ether, 20 grams of stabilizer dibutyl hydroxy toluene, 30 grams of 2,6-di-tert-butyl p-cresol, oil base
829.8 grams of salad oil.
In another embodiment, component is formulated as 20,000,000,000 spore of Metarhizium anisopliae/90 grams of g high cryptogams, cyromazine
10.2 grams, 50 grams of 50 grams of emulsifier triethanolamine list olein, castor oil and ethylene oxide condensate, wetting agent alkyl phenolic group gather
100 grams of oxyethylene group ether, 30 grams of stabilizer propylgallate, 450 grams and 219.8 grams of oil base paraffin oil.
Embodiment 3：Metarhizium anisopliae cyromazine water dispersible granules (the conidial number of Metarhizium anisopliae and institute
The grams ratio for stating cyromazine is 1 × 2 × 1010：9)
Weigh 20,000,000,000 spores of Metarhizium anisopliae/10 grams of g cryptogams, 92 grams of cyromazine, the polyether-modified poly-organosilicon of wetting agent
120 grams of oxygen alkane, 100 grams of dispersant alkyl naphthalene formaldehyde condensate sulfonate, 80 grams of fatty alcohol polyoxyethylene ether；Disintegrant ammonium sulfate
60 grams, 10 grams of adhesive starch, 10 grams of stabilizer butylated hydroxyarisol, surplus be diatomite supply gross weight as carrier
Amount is 1000 grams.
By the Metarhizium anisopliae conidia powder, the cyromazine, the wetting agent, the dispersant, the disintegrant,
After the stabilizer and the carrier proportionally mix coarse crushing, is crushed with airslide disintegrating mill and superfine powder is made, gained is surpassed
Fine powder mixes the binder in fluidized bed pelletizer and is granulated, and is sieved, is obtained water-dispersed through vibrating screen after dry
Granula Pesticidal combination.
In another embodiment, component is formulated as 20,000,000,000 spores of Metarhizium anisopliae/10 grams of g cryptogams, cyromazine 92
Gram, 10 grams of wetting agent lauryl sodium sulfate, 10 grams of dispersant lignosulfonates, 10 grams of modified lignin mahogany sulfonate, disintegration
5 grams of agent sodium bicarbonate, 5 grams of bentonite, 5 grams of binder paraffin oil, surplus are that supply total weight as carrier be 1000 to kaolin
Gram.
In another embodiment, component is formulated as 20,000,000,000 spores of Metarhizium anisopliae/10 grams of g cryptogams, cyromazine 92
Gram, 30 grams of wetting agent fatty acid sulfates, 120 grams of fatty alcohol polyoxyethylene ether, 200 grams of dispersant naphthalene sulfonic acid-formaldehyde condensation product；
120 grams of disintegrant urea, 60 grams of magnesium sulfate, 10 grams of binder polyethylene glycol, 30 grams of polyvinyl alcohol, 10 grams of cyclodextrin, stabilizer
30 grams of 2,6-di-tert-butyl p-cresol, 20 grams of propylgallate, surplus is white carbon, attapulgite supplies gross weight as carrier
Amount is 1000 grams.
In another embodiment, component is formulated as 20,000,000,000 spores of Metarhizium anisopliae/10 grams of g cryptogams, cyromazine 92
Gram, 80 grams of wetting agent alkyl phenolic group polyoxyethylene ether, 50 grams of dispersant nekal, lignosulfite 50
Gram；40 grams of disintegrant sodium carbonate, 30 grams of aluminium chloride, 30 grams of sucrose, 5 grams of binder sodium metasilicate, 5 grams of phenolic resin and Methyl cellulose
10 grams of element, 20 grams of stabilizer tert-butylhydroquinone, surplus are that supply total weight as carrier be 1000 grams to bentonite.
Embodiment 4：The embodiment of the present invention is the method being combined using indoor virulence test and field trial.
Test reagent：Metarhizium anisopliae 20,000,000,000 live spore/gram, cyromazine active compound 98%.
Subjects：
Filter paper is moistened with the liquid of various concentration, is layered in 9cm culture dishes, it is small that leek (white portion) is cut into 2cm
Section, impregnates 15s in various concentration liquid.It takes out 5 sections to be placed in culture dish, then lightly be moved into writing brush of the same size
Third-instar larvae.20 are often handled, is repeated 4 times, while setting clear water control, while clear water is supplemented with manual sprayer, keeps filter paper
Moistening, is replenished in time the leek stem of dipped respective concentration liquid, finally calculates the death rate daily.
It is with SPSS data processing softwares for statistical analysis, the LC50 of each medicament is calculated, reagent agent is evaluated with this
Activity.
The co-toxicity coefficient (CTC values) of mixture is calculated according to the following formula：
In formula：
ATI- mixture surveys toxicity indexes；
The LC50 of S- standard pesticide agent, unit are milligrams per liter；
The LC50 of M- mixtures, unit are milligrams per liter.
TTI=TIA × PA+TIB × PB
In formula：
TTI-mixtures theoretical toxicity index；
TIA-A medicament toxicity index；
Percentage composition of PA-A medicaments in mixture, unit are percentage；
TIB-B medicament toxicity index；
Percentage composition of PB-B medicaments in mixture, unit are percentage.
In formula：
CTC-co-toxicity coefficient；
AT-mixture surveys toxicity index；
TTI-mixtures theoretical toxicity index.
The co-toxicity coefficient (CTC) of compounding agent：
CTC >=120 show as synergistic effect；
CTC≤80 show as antagonism；
80 ＜ CTC ＜ 120 show as summation action.
Table 1 is the difference of the Pesticidal combination of the Metarhizium anisopliae (A) and cyromazine (B) containing different proportion of the present invention
Activity (the LC of reagent agent50), the testing result of co-toxicity coefficient (CTC) and mixtures theoretical toxicity index (TTI).Table 1 is interior
Toxicity test is as a result, the mass ratio of the high conidia powder of Metarhizium anisopliae and cyromazine is (49-1) it can be seen from 1 result of table：
(1-49) shows apparent synergistic effect, wherein with the high conidia powder of Metarhizium anisopliae：Cyromazine (mass ratio)=1：49
Synergistic effect is most apparent.
Virulence significantly improves, efficacy stability, and quick-acting is good；The usage amount of chemical pesticide is reduced, and the usage amount of green muscardine fungus subtracts
It is few；Insect is not likely to produce drug resistance；The control effect having had to the ovum of pest, larva, pupa, adult.
Table 1
Embodiment 5：Store spore Activity determination
Peptone potato dextrose agar (PPDA) is applied directly on glass slide, then takes and contains green deadlock a little
Composition in the embodiment of the present invention 1,2 and 3 of bacterium conidial powder, 1 × 10 is diluted to the sterile water containing 0.1% glycerine7
A/mL is uniformly smeared, 28 DEG C of moisturizing 36h after 20 μ L spore suspensions are added dropwise.Each processing counts 300 or so spores, general
It holds high office and observes and records spore germination situation under 400 times of micro mirror, calculate spore germination rate.Table 2 is spore germination rate as a result, by this
Table is not as it can be seen that cyromazine and other adjuvants in the present composition be active to the spore of Metarhizium anisopliae in composition
It causes significantly to influence.
Table 2
| Store forespore germination rate, % | 25 DEG C of indoor storages, 180 days spore germination rates, % | |
| Example 1 | 95.8 | 88.3 |
| Example 2 | 97.2 | 92.4 |
| Example 3 | 96.3 | 90.7 |
Embodiment 6：Field information is tested：
This experiment is arranged in Shouguang City of Shandong Province towns Tian Liu, and test medicine is ground by Guangdong new scene bioengineering Co., Ltd
Hair provides, 10,000,000,000 spore of comparison medicament/gram Metarhizium anisopliae wettable powder (purchased in market), 10% cyromazine suspending agent (city
Purchase).
When latter stage occurs for Bradysia fungus gnat adult, will uniformly be poured in leek root after 500 times of dilutions of medicament, if clear water pair
According to each processing is repeated 4 times.Each plot area 20m2The leek of 1/4 area of each cell is connected root before dispenser to dig out, with root
For the number of portion's bradysia odoriphaga as radix, 7 days, 15 days, 30 days, 60 days leek for digging out 1/4 area again after medicine count root bradysia odoriphaga
Number, table 3 are preventive effect result of calculation, by the table as it can be seen that the present composition is for dose pouring root 7d, 15d, 30d, 60d on probation
Field control effect reaches 80% or more, and quick-acting is good, and the lasting period is long, and minimum preventive effect is 80.10%, and highest preventive effect is
96.10%, the control effect with obvious effects better than single dose.The present composition reduces medication while improving preventive effect
Amount.
Table 3
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than is protected to the present invention
The limitation of range is protected, although being explained in detail to the present invention with reference to preferred embodiment, those skilled in the art should
Understand, technical scheme of the present invention can be modified or replaced equivalently, without departing from the essence of technical solution of the present invention
And range.
Claims (10)
1. a kind of Pesticidal combination containing Metarhizium anisopliae and cyromazine, which is characterized in that the Pesticidal combination contains gold
Tortoise green muscardine fungus and cyromazine, the Metarhizium anisopliae and the cyromazine are mixed in following ratio：The green deadlock of chafer
Conidial number of bacterium is (49-1) × 2 × 10 with the grams ratio of the cyromazine10：(1-49).
2. Pesticidal combination according to claim 1, which is characterized in that the composition is by Metarhizium anisopliae, fly eradication
Amine and adjuvant composition.
3. Pesticidal combination according to claim 2, which is characterized in that the adjuvant is wetting agent, dispersant, stabilizer
And carrier, the percentage for the total weight that the weight of the adjuvant each component accounts for the Pesticidal combination are respectively：
The wetting agent accounts for 5-35%；
The dispersant accounts for 5-30%；
The stabilizer accounts for 0%-5%；
The carrier complements to 100%；
The wetting agent is TERWET1004, neopelex, alkylnaphthalene sulfonate and anion humectant mixture
It is one or more in MorwetEFW, tea seed cake, spaonin powder, spaonin powder, silkworm excrement and pull open powder；
The dispersant is lignosulfonates, modified lignin mahogany sulfonate, naphthalene sulfonic acid-formaldehyde condensation product, alkyl naphthalene formaldehyde condensation
It is one or more in object sulfonate, fatty alcohol polyoxyethylene ether, nekal and lignosulfite；
The stabilizer is butylated hydroxyarisol, 2,6-di-tert-butyl p-cresol, propylgallate, tertiary butyl to benzene
It is one or more in diphenol；
The carrier is one or more in kaolin, white carbon, attapulgite, diatomite, bentonite.
4. the preparation method of the Pesticidal combination described in claim 3, which is characterized in that by the cyromazine, the wetting agent,
The dispersant, the stabilizer and the carrier are uniformly mixed in premixing tank, are made ultra-fine after being smashed by air jet mill
Powder is uniformly mixed with the Metarhizium anisopliae conidia powder, obtains wettable powder Pesticidal combination.
5. Pesticidal combination according to claim 2, which is characterized in that the adjuvant is wetting agent, dispersant, disintegration
Agent, binder, stabilizer and carrier；The weight of the adjuvant each component accounts for the percentage difference of the Pesticidal combination total weight
For：
The wetting agent accounts for 1-15%；
The dispersant accounts for 2-20%；
The disintegrant accounts for 1-18%；
The binder accounts for 1-5%；
The stabilizer accounts for 0%-5%；
The carrier complements to 100%；
The wetting agent is polyether-modified polysiloxane, lauryl sodium sulfate, fatty acid sulfates, fatty alcohol polyoxy second
It is one or more in alkene ether and alkyl phenolic group polyoxyethylene ether；
The dispersant is lignosulfonates, modified lignin mahogany sulfonate, naphthalene sulfonic acid-formaldehyde condensation product, alkyl naphthalene formaldehyde condensation
It is one or more in object sulfonate, fatty alcohol polyoxyethylene ether, nekal and lignosulfite；
The disintegrant is one kind in sodium bicarbonate, bentonite, ammonium sulfate, urea, magnesium sulfate, sodium carbonate and chlorination aluminum sucrose
Or it is a variety of；
The binder is that paraffin oil, polyethylene glycol, polyvinyl alcohol, cyclodextrin, starch, sodium metasilicate, phenolic resin and methyl are fine
It is one or more in dimension element；
The stabilizer is butylated hydroxyarisol, 2,6-di-tert-butyl p-cresol, propylgallate and tertiary butyl pair
It is one or more in benzenediol；
The carrier is one or more in kaolin, white carbon, attapulgite, diatomite and bentonite.
6. the preparation method of the Pesticidal combination described in claim 5, which is characterized in that by the Metarhizium anisopliae spore
Powder, the cyromazine, the wetting agent, the dispersant, the disintegrant, the stabilizer and the carrier proportionally mix
It after closing coarse crushing, is crushed with airslide disintegrating mill and superfine powder is made, gained superfine powder mixed in fluidized bed pelletizer described viscous
Knot agent is granulated, and is sieved through vibrating screen after dry, obtains water dispersible granules Pesticidal combination.
7. Pesticidal combination according to claim 2, which is characterized in that the adjuvant is wetting agent, emulsifier, stabilizer
And oil base, the percentage for the total weight that the weight of the adjuvant each component accounts for the Pesticidal combination are respectively：
The wetting agent accounts for 1-15%；
The emulsifier accounts for 1-15%；
The stabilizer accounts for 0%-5%；
The oil base supplies 100%；
The wetting agent is polyether-modified polysiloxane, lauryl sodium sulfate, fatty acid sulfates, fatty alcohol polyoxy second
It is one or more in alkene ether and alkyl phenolic group polyoxyethylene ether；
The emulsifier is sorbitan monooleate, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol and epoxy second
One kind in alkane condensation product, polyoxyethylene fatty acid ester, triethanolamine list olein and castor oil and ethylene oxide condensate or
It is several；
The stabilizer is dibutyl hydroxy toluene, 2,6-di-tert-butyl p-cresol, propylgallate and tertiary butyl to benzene two
It is one or more in phenol；
The oil base is one or more in rapeseed oil, sesame oil, peanut oil, cottonseed oil, salad oil and paraffin oil.
8. Pesticidal combination according to claim 7, which is characterized in that the dosage form of the composition is oil-suspending agent, institute
The grams ratio for stating the conidial number of Metarhizium anisopliae and the cyromazine is 9 × 2 × 1010：1.
9. the preparation method of the Pesticidal combination described in claim 7, which is characterized in that by the cyromazine, the wetting agent,
The emulsifier, the stabilizer and the oil base sequentially add in mixing kettle, stir evenly, after the shearing of high shear dispersion kettle
It is sanded into sand mill, sand milling to 90% grain size is less than after 5 microns to be uniformly mixed with the muscardine spore powder, obtains oil
Suspending agent disinfestation composition.
10. the Pesticidal combination described in one of claim 1-9 is in prevention scale insect, trialeurodes vaporariorum, thrips, grub, wireworm, tangerine
Application in small trypetid, liriomyza bryoniae, root maggot, bradysia odoriphaga and Bradysia odoriphaga.
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| WO2020207995A1 (en) * | 2019-04-12 | 2020-10-15 | Bayer Cropscience Biologics Gmbh | Methods of increasing the germination rate of fungal spores |
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| AU2019300109A1 (en) * | 2018-07-10 | 2021-01-21 | Bayer Aktiengesellschaft | Novel carrier fluids for liquid fungal spore formulations |
| CN110547304A (en) * | 2019-09-24 | 2019-12-10 | 重庆谷百奥生物研究院有限公司 | Beauveria bassiana and amitraz compounded trialeurodes vaporariorum killing composition |
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|---|---|---|---|---|
| CN1788569A (en) * | 2005-12-18 | 2006-06-21 | 刘起麟 | Preparation and uses of false proof highly-effective insecticide and bactericide |
| WO2015069708A1 (en) * | 2013-11-08 | 2015-05-14 | Novozymes Bioag A/S | Compositions and methods for treating pests |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1788569A (en) * | 2005-12-18 | 2006-06-21 | 刘起麟 | Preparation and uses of false proof highly-effective insecticide and bactericide |
| WO2015069708A1 (en) * | 2013-11-08 | 2015-05-14 | Novozymes Bioag A/S | Compositions and methods for treating pests |
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| Evaluation of alternative rice planthopper control by the combined action of oil-formulated Metarhizium anisopliae and low-rate buprofezin;Shao-Feng Jin;《Pest Manag. Sci. 第67卷》;20101014;36-43 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2020207995A1 (en) * | 2019-04-12 | 2020-10-15 | Bayer Cropscience Biologics Gmbh | Methods of increasing the germination rate of fungal spores |
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