CN117441746B - Application of mountain pepper alcohol extract in killing agricultural pests and/or preparing broad-spectrum biological control product - Google Patents
Application of mountain pepper alcohol extract in killing agricultural pests and/or preparing broad-spectrum biological control product Download PDFInfo
- Publication number
- CN117441746B CN117441746B CN202311397223.1A CN202311397223A CN117441746B CN 117441746 B CN117441746 B CN 117441746B CN 202311397223 A CN202311397223 A CN 202311397223A CN 117441746 B CN117441746 B CN 117441746B
- Authority
- CN
- China
- Prior art keywords
- mountain pepper
- alcohol extract
- biological control
- alcohol
- mountain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 126
- 235000008496 Drimys aromatica Nutrition 0.000 title claims abstract description 64
- 240000002262 Litsea cubeba Species 0.000 title claims abstract description 64
- 235000012854 Litsea cubeba Nutrition 0.000 title claims abstract description 64
- 239000000284 extract Substances 0.000 title claims abstract description 61
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 29
- 230000002147 killing effect Effects 0.000 title abstract description 23
- 241000927584 Frankliniella occidentalis Species 0.000 claims abstract description 34
- 241001454293 Tetranychus urticae Species 0.000 claims abstract description 33
- 241000346285 Ostrinia furnacalis Species 0.000 claims abstract description 32
- 241001124076 Aphididae Species 0.000 claims abstract description 27
- 238000002360 preparation method Methods 0.000 claims description 19
- 239000000126 substance Substances 0.000 claims description 19
- 238000002386 leaching Methods 0.000 claims description 18
- 238000005507 spraying Methods 0.000 claims description 18
- 238000002137 ultrasound extraction Methods 0.000 claims description 16
- 241000238631 Hexapoda Species 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 12
- 238000002604 ultrasonography Methods 0.000 claims description 11
- 235000013399 edible fruits Nutrition 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 235000011837 pasties Nutrition 0.000 claims description 5
- 241001658152 Lepidium montanum Species 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 4
- 244000245109 Lindera glauca Species 0.000 claims description 2
- 235000001577 Lindera glauca Nutrition 0.000 claims description 2
- 241000209140 Triticum Species 0.000 abstract description 23
- 235000021307 Triticum Nutrition 0.000 abstract description 23
- 230000000694 effects Effects 0.000 abstract description 7
- 238000002474 experimental method Methods 0.000 abstract description 6
- 231100000419 toxicity Toxicity 0.000 description 25
- 230000001988 toxicity Effects 0.000 description 25
- 230000000052 comparative effect Effects 0.000 description 18
- 239000000469 ethanolic extract Substances 0.000 description 18
- 241001646398 Pseudomonas chlororaphis Species 0.000 description 16
- 238000000034 method Methods 0.000 description 16
- 238000011282 treatment Methods 0.000 description 15
- 238000004090 dissolution Methods 0.000 description 12
- 239000012681 biocontrol agent Substances 0.000 description 11
- 239000003814 drug Substances 0.000 description 11
- 239000007921 spray Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 8
- 231100000820 toxicity test Toxicity 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- IYRMWMYZSQPJKC-UHFFFAOYSA-N kaempferol Chemical compound C1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 IYRMWMYZSQPJKC-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- VBIRCRCPHNUJAS-AFHBHXEDSA-N 4-[(1S,3aR,4S,6aR)-4-(1,3-benzodioxol-5-yl)tetrahydrofuro[3,4-c]furan-1-yl]-2-methoxyphenol Chemical compound C1=C(O)C(OC)=CC([C@@H]2[C@@H]3[C@@H]([C@H](OC3)C=3C=C4OCOC4=CC=3)CO2)=C1 VBIRCRCPHNUJAS-AFHBHXEDSA-N 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 5
- VPSRGTGHZKLTBU-UHFFFAOYSA-N piperitol Natural products COc1ccc(cc1OCC=C(C)C)C2OCC3C2COC3c4ccc5OCOc5c4 VPSRGTGHZKLTBU-UHFFFAOYSA-N 0.000 description 5
- 230000000607 poisoning effect Effects 0.000 description 5
- 238000000967 suction filtration Methods 0.000 description 5
- HPOHAUWWDDPHRS-UHFFFAOYSA-N trans-piperitol Natural products CC(C)C1CCC(C)=CC1O HPOHAUWWDDPHRS-UHFFFAOYSA-N 0.000 description 5
- 230000001018 virulence Effects 0.000 description 5
- BURBOJZOZGMMQF-UHFFFAOYSA-N xanthoxylol Natural products C1=C(O)C(OC)=CC=C1C1C(COC2C=3C=C4OCOC4=CC=3)C2CO1 BURBOJZOZGMMQF-UHFFFAOYSA-N 0.000 description 5
- 241000722363 Piper Species 0.000 description 4
- 241000125167 Rhopalosiphum padi Species 0.000 description 4
- 229960000074 biopharmaceutical Drugs 0.000 description 4
- 230000000875 corresponding effect Effects 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 238000007873 sieving Methods 0.000 description 4
- UBSCDKPKWHYZNX-UHFFFAOYSA-N Demethoxycapillarisin Natural products C1=CC(O)=CC=C1OC1=CC(=O)C2=C(O)C=C(O)C=C2O1 UBSCDKPKWHYZNX-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 241000746609 Piper hancei Species 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 3
- 235000008777 kaempferol Nutrition 0.000 description 3
- 238000000622 liquid--liquid extraction Methods 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 239000002021 butanolic extract Substances 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 244000307888 Acanthus ebracteatus Species 0.000 description 1
- 241000239290 Araneae Species 0.000 description 1
- 235000005781 Avena Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000218195 Lauraceae Species 0.000 description 1
- 241000223201 Metarhizium Species 0.000 description 1
- 241000721621 Myzus persicae Species 0.000 description 1
- 240000005546 Piper methysticum Species 0.000 description 1
- 235000016787 Piper methysticum Nutrition 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 241000180197 Sitobion Species 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 244000062793 Sorghum vulgare Species 0.000 description 1
- 241001454295 Tetranychidae Species 0.000 description 1
- 241000344246 Tetranychus cinnabarinus Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012259 ether extract Substances 0.000 description 1
- 239000002024 ethyl acetate extract Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 235000021251 pulses Nutrition 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Abstract
The invention belongs to the technical field of biological control, and in particular relates to application of a mountain pepper alcohol extract in killing agricultural pests and/or preparing a broad-spectrum biological control product. The alcohol extract of the mountain pepper is used for killing the wheat long tube aphids, the two-spotted spider mites, the frankliniella occidentalis and the Asiatic corn borer, so that the biological control effect can be achieved, and the environment-friendly and safe effect can be achieved. Experiments show that the mortality rate of the wheat long tube aphid, the two-spotted spider mite, the frankliniella occidentalis and the Asiatic corn borer is up to 85.9 percent by adopting the technical scheme provided by the invention.
Description
Technical Field
The invention belongs to the technical field of biological control, and in particular relates to application of a mountain pepper alcohol extract in killing agricultural pests and/or preparing a broad-spectrum biological control product.
Background
The agricultural insect pests with wide occurrence area, strong destructive power and serious harm are prepared from the wheat long tube aphid, the two-spotted spider mites, the frankliniella occidentalis and the Asian corn borer. For example, the wheat aphid has occurrence in the main wheat area of China, and is a dominant species in wheat aphid; asiatic corn borers belong to the world pests which harm crops such as corn, sorghum, millet and the like; and spider mites and frankliniella occidentalis have the characteristics of wide host range, rapid propagation, high concealment and strong drug resistance.
At present, chemical agents are mostly adopted for pest control, however, the chemical agents also have killing effect on non-target organisms while controlling pests, so that ecological balance is destroyed, and pests are caused to be rampant again. Furthermore, although there are reports on the preparation of botanical pesticides in the prior art, the pesticidal spectrum is narrow. For example, chinese patent CN112674121a only reports that acanthus trifoliatus essential oil is used for killing frankliniella occidentalis or tetranychus urticae.
Fructus Piperis (Lindera glauca (Siebold & zucc.) Blume), genus Piper of family Lauraceae. The fallen leaves of shrubs or small trees can reach 8 meters; bark is smooth, grey or off-white. She Husheng wide oval shape with dark green upper surface and light green lower surface, white soft fur, paper, feathered pulse; the stem is 1-1.5 cm long, the flowering period is 3-4 months, the fruiting period is 7-8 months, and the stem is widely distributed in a plurality of provinces in China. However, the application of peppers in killing of myzus persicae, tetranychus urticae, frankliniella occidentalis and Asiatic corn borers has not been reported in the art.
Disclosure of Invention
The invention aims to provide the application of the mountain pepper alcohol extract in killing agricultural pests and/or preparing broad-spectrum biological control products, and the mountain pepper alcohol extract can kill and kill long-tube aphid, two-spotted spider mites, frankliniella occidentalis and Asiatic corn borer simultaneously, and has good broad-spectrum property.
The invention provides application of a mountain pepper alcohol extract in killing agricultural pests and/or preparing a broad-spectrum biological control product; the species of agricultural pests include: one or more of Long tube aphids, two-spotted spider mites, frankliniella occidentalis and Asiatic corn borers.
Preferably, the relative content of each substance in the mountain pepper alcohol extract is shown in the following table:
preferably, the preparation method of the mountain pepper alcohol extract comprises the following steps:
mixing a mountain pepper sample with alcohol, and performing first ultrasonic extraction to obtain a first leaching solution and first filter residues respectively;
Mixing the first filter residue with alcohol again for second ultrasonic extraction to obtain a second leaching solution;
mixing the first leaching solution and the second leaching solution, and concentrating into pasty substances to obtain the mountain pepper alcohol extract.
Preferably, the mountain pepper sample is selected from one or more of the roots, stems, leaves and fruits of mountain pepper plants.
Preferably, the alcohol comprises 70-100% ethanol solution by volume percent; the weight-volume ratio of the mountain pepper sample to the ethanol solution is 0.5-1.5 g: 10-40 mL.
Preferably, the time of the first ultrasonic wave and the second ultrasonic wave is respectively 20-50 min.
Preferably, when spraying insects, alcohol ultrasonic is utilized to dissolve the mountain pepper alcohol extract to obtain mountain pepper alcohol extract;
the concentration of the mountain pepper alcohol extract is 0.2-1 g/mL.
The invention provides a broad-spectrum biological control medicament, and the active ingredients of the biological control medicament comprise a mountain pepper alcohol extract.
The invention also provides application of the biological control agent in pest control.
The beneficial effects are that:
The invention provides application of a mountain pepper alcohol extract in killing agricultural pests and/or preparing a broad-spectrum biological control product; the species of agricultural pests include: one or more of Long tube aphids, two-spotted spider mites, frankliniella occidentalis and Asiatic corn borers. The alcohol extract of the mountain pepper is used for killing agricultural pests, can not kill the wheat long tube aphid, the two-spotted spider mites, the frankliniella occidentalis and the Asiatic corn borer at the same time, and is green and safe.
Based on the technical advantages, the invention also provides a broad-spectrum biological control medicament, and the active ingredient of the biological control medicament is a mountain pepper alcohol extract. The biological control agent containing the mountain pepper alcohol extract is used for biological control of pests, so that the death rate of the pests is improved. Experiments show that the mortality rate of the wheat long tube aphid, the two-spotted spider mite, the frankliniella occidentalis and the Asiatic corn borer is up to 86.7 percent by adopting the technical scheme provided by the invention.
Detailed Description
The invention provides application of a mountain pepper alcohol extract in killing agricultural pests and/or preparing a broad-spectrum biological control product; the insect species include: one or more of Long tube aphids, two-spotted spider mites, frankliniella occidentalis and Asiatic corn borers. The alcohol extract of the mountain pepper is used for killing pests, can not kill the wheat long tube aphid, the two-spotted spider mites, the frankliniella occidentalis and the Asiatic corn borer at the same time, and is green and safe.
In the invention, the agricultural insect is preferably one or more of wheat long tube aphid, tetranychus urticae, frankliniella occidentalis and Asian corn borer, more preferably wheat long tube aphid, tetranychus urticae, frankliniella occidentalis and Asian corn borer; the content of each substance and the relative content of each substance in the mountain pepper alcohol extract are shown in table 1:
TABLE 1 relative percentage of substances in the ethanol extract of Piper hancei
The alcohol extract of the mountain pepper is used for killing pests, and can achieve the effect of killing the wheat long tube aphid, the two-spotted spider mites, the frankliniella occidentalis and the Asiatic corn borer simultaneously.
The invention preferably prepares a mountain pepper alcohol extract, and the preparation method of the mountain pepper alcohol extract preferably comprises the following steps: mixing a mountain pepper sample with alcohol, and performing first ultrasonic extraction to obtain a first leaching solution and first filter residues respectively; mixing the first filter residue with alcohol again for second ultrasonic extraction to obtain a second leaching solution; mixing the first leaching solution and the second leaching solution, and concentrating into pasty substances to obtain the mountain pepper alcohol extract.
The present invention preferably dries, pulverizes and sieves the mountain pepper sample. In the present invention, the mountain pepper sample is preferably one or more of roots, stems, leaves and fruits of a mountain pepper plant, more preferably an entire mountain pepper plant containing roots, stems, leaves and fruits. The temperature of the drying is preferably 35-45 ℃, more preferably 40 ℃; the drying time is not particularly required, and the sample is completely dried. The pulverizing method and apparatus of the present invention are not particularly limited, and may be carried out by techniques well known in the art. In the present invention, the pore diameter of the sieve at the time of sieving is preferably 0.1 to 0.8mm, more preferably 0.4mm. The powder sample with proper particle size can be obtained by drying, crushing and sieving.
After the sieving, the invention preferably mixes the sieved powdery sample with alcohol and then carries out the first ultrasonic extraction. In the present invention, the alcohol is preferably an ethanol solution; the volume percentage of the ethanol solution is preferably 70-100%, more preferably 95%; the weight-to-volume ratio of the sieved sample to the ethanol solution is preferably 0.5-1.5 g:10 to 40mL, more preferably 1g:10mL. The mixing time according to the invention is preferably 20 to 28 hours, more preferably 24 hours. Through reasonable mixing and soaking, precipitation of effective active substances in the sample is facilitated.
The time of the first ultrasonic wave is preferably 20-50 min, more preferably 30min; the power of the first ultrasound is preferably 200 to 300W, more preferably 270W; the temperature of the first ultrasound is preferably 20 to 25 ℃, more preferably 23 ℃. The present invention is not particularly limited to the first ultrasound device, and may be implemented by any device known in the art. In a specific embodiment of the invention, the first ultrasonic device is a KQ-300DE digital controlled ultrasonic cleaner, purchased from Kunshan ultrasonic instruments Inc.
After the first ultrasonic extraction, the invention preferably carries out solid-liquid separation on the mixture after the first ultrasonic extraction to respectively obtain a first leaching solution and first filter residues. The solid-liquid separation mode is preferably suction filtration, and the suction filtration is finished when no leaching liquor is extracted.
After the solid-liquid separation, the first filter residue and the alcohol are mixed again for the second ultrasonic extraction, and more preferably, the first filter residue and the alcohol are mixed again for the second ultrasonic extraction immediately. In the present invention, the alcohol is preferably an ethanol solution upon remixing; the usage amount and the volume percentage of the ethanol solution during remixing are the same as the usage amount range and the volume percentage range of the ethanol solution in the technical scheme, and are not repeated here.
The time, power, temperature and equipment of the second ultrasound are identical to those of the first ultrasound, and the time, power, temperature and equipment of the first ultrasound are described in detail above and are not described in detail herein.
After the second ultrasonic extraction, the invention preferably carries out solid-liquid separation again on the mixture after the second ultrasonic extraction to obtain a second leaching solution. The solid-liquid separation method of the invention is the same as the solid-liquid separation method in the above technical scheme, and will not be described here again.
After the secondary solid-liquid separation, the present invention preferably mixes the first leaching solution and the second leaching solution and concentrates the mixture into a pasty substance. In the invention, the concentration process is finished when no leaching liquor is evaporated; the mixing and concentrating equipment is not particularly limited and may be carried out by techniques well known in the art. In a specific embodiment of the present invention, a rotary evaporator is used for the concentration.
After the concentration, the present invention preferably utilizes alcohol sonication to dissolve the pasty material. In the present invention, the alcohol is preferably an ethanol solution; the volume percentage of the ethanol solution is the same as the volume percentage range of the ethanol solution in the above technical scheme, and will not be described herein. The volume mass ratio of the ethanol solution used in ultrasonic dissolution to the screened mountain pepper sample in the technical scheme is 1mL:1g.
In the invention, the concentration of the mountain pepper alcohol extract is preferably calculated according to the mass of mountain pepper samples and the volume of ethanol solution during ultrasonic dissolution, namely 1g/mL mountain pepper alcohol extract obtained by extracting 1g mountain pepper samples is prepared by ultrasonic dissolution in 1mL ethanol solution with the volume fraction of 95%; the concentration of the mountain pepper alcohol extract is preferably 0.2-1 g/mL, more preferably 1g/mL. The mountain pepper alcohol extract can be directly used for spraying and killing pests by dissolving the mountain pepper alcohol extract.
The invention also provides a broad-spectrum biological control medicament, and the active ingredient of the biological control medicament is a mountain pepper alcohol extract.
The preferred relative percentages of each substance in the mountain pepper alcohol extract are shown in table 1:
TABLE 1 relative percentage of substances in the ethanol extract of Piper hancei
In the invention, the biological control is preferably killing insects, and the type of the insects is preferably one or more of wheat long tube aphid, tetranychus urticae, frankliniella occidentalis and Asian corn borer, and more preferably wheat long tube aphid, tetranychus urticae, frankliniella occidentalis and Asian corn borer. The biological control agent is prepared by the mountain pepper alcohol extract containing the active components, so that the biological control effect can be achieved.
The dosage form, auxiliary materials and the using mode of the biological control medicament are not particularly required, and any biological control medicament containing the mountain pepper alcohol extract is within the protection scope of the invention.
The invention also provides application of the biological control medicament in pest control. Experiments show that the technical scheme provided by the invention not only can achieve the biological control effect of killing the wheat long tube aphid, the two-spotted spider mites, the frankliniella occidentalis and the Asiatic corn borer at the same time, but also has the death rate of 86.7 percent, good control effect and environment friendliness and safety.
For further explanation of the present invention, the application of the alcohol extract of Piper hanensis provided in the present invention to the killing of agricultural pests and/or the preparation of broad-spectrum biocontrol products is described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Preparation before experiment:
Test insects: sitobion avena (Fabrices), tetranychus urticae Tetranychus cinnabarinus, frankliniella occidentalis FRANKLINIELLA OCCIDENTALIS (Pergande) and Asian corn borer Ostrinia furnacalis (Guen ee) are all indoor raising populations, and raising conditions are that the temperature is 25+/-1 ℃, the humidity is 75+/-5%, and the photoperiod is 12D:12L, provided by the institute of plant protection, the academy of agrology and biotechnology and the academy of agricultural sciences, china.
Example 1
The preparation method of the mountain pepper alcohol extract comprises the following steps:
1) Test plants: drying fructus Piperis in oven at 40deg.C, pulverizing, sieving with 0.400mm sieve, and packaging in sealed bag. Mountain pepper was purchased from Anguo cold back medicine limited in Hebei province.
2) Extracting: accurately weighing 50g of the dried plant sample in the step 1), placing the dried plant sample into a 1000mL beaker, adding 500mL of ethanol solution (the volume percentage is 95%), soaking for 24 hours, performing first ultrasonic extraction on the mixture of the dried plant sample and the ethanol solution, and extracting for 30 minutes under the conditions of 90Hz power and 23 ℃ temperature (ultrasonic equipment model is KQ-300DE numerical control ultrasonic cleaner purchased from Kunshan ultrasonic instrument Co., ltd.). Performing suction filtration (SHZ-DIII multipurpose vacuum pump suction filtration) after the first ultrasonic extraction until no ethanol in the soaked residues flows out to obtain first ultrasonic filtrate and first ultrasonic filter residues respectively, and reserving the first ultrasonic filtrate;
Adding 500mL of ethanol solution (volume percentage is 95%) into the first ultrasonic filter residue, performing second ultrasonic extraction for 30min under the conditions of power of 90Hz and temperature of 23 ℃, and performing suction filtration (SHZ-DIII multipurpose vacuum pump filtration) again until no ethanol in the soaked residues flows out to obtain second ultrasonic filtrate.
The first and second ultrasonic filtrates were combined and concentrated to an extract by rotary evaporator until no extract had evaporated. The obtained extractum-like substance was dissolved in 50mL of ethanol solution (95% by volume) while the ultrasound was assisted until complete dissolution. Thus, 1g/mL of a kaempferol extract was obtained and put in a refrigerator for use.
Performing non-target metabonomics detection and identification (completed by entrusted Xinjie Chuanghui technology (Beijing) Co., ltd.) on the effective components of the obtained kava alcohol extract, and obtaining corresponding data according to the peak area ratio of the measurement result, namely, the normalization treatment, namely, the peak area percentage of the detected substances, wherein the result is shown in Table 1:
TABLE 1 relative percentage of substances in the ethanol extract of Piper hancei
Comparative example 1
The preparation of petroleum ether extract liquid comprises the following steps:
50g of mountain pepper fruit was extracted according to the extraction method in example 1 to obtain an extract-like substance. Adding 500mL of distilled water into the extractum-like substance for hot dissolution, loading into a separating funnel after dissolution, and continuously distributing for 3 times by using 50mL of petroleum ether by adopting a liquid-liquid distribution method to obtain corresponding fractions respectively. The fractions obtained 3 times were then combined and concentrated again under reduced pressure to give a thick paste. Then, 50mL of a 95% ethanol solution was subjected to ultrasonic dissolution to obtain 1g/mL of ethanol extract A.
Comparative example 2
The preparation of the ethyl acetate extract comprises the following steps:
50g of mountain pepper fruit was extracted according to the extraction method in example 1 to obtain an extract-like substance. Adding 500mL of distilled water into the extractum-like substance for hot dissolution, loading into a separating funnel after dissolution, and continuously distributing for 3 times by using 50mL of ethyl acetate by adopting a liquid-liquid distribution method to obtain corresponding fractions respectively. The fractions obtained 3 times were then combined and concentrated again under reduced pressure to give a thick paste. Ultrasonic dissolution was performed with 50mL of 95% ethanol solution to give 1g/mL ethanol extract B.
Comparative example 3
The preparation of the n-butanol extract comprises the following steps:
50g of mountain pepper fruit was extracted according to the extraction method in example 1 to obtain an extract-like substance. Adding 500mL of distilled water into the extractum-like substance for hot dissolution, loading into a separating funnel after dissolution, and continuously distributing for 3 times by using 50mL of n-butanol by adopting a liquid-liquid distribution method to obtain corresponding fractions respectively. The fractions obtained 3 times were then combined and concentrated again under reduced pressure to give a thick paste. Then, the mixture was sonicated with 50mL of a 95% ethanol solution to obtain 1g/mL of ethanol extract C.
Application example 1
A. Contact toxicity test of Tetranychus urticae
1) Preparation of biological control agents: 1g/mL of the mountain pepper alcohol extract, 1g/mL of the ethanol extract A, 1g/mL of the ethanol extract B and 1g/mL of the ethanol extract C prepared in example 1 and comparative examples 1 to 3 were taken, respectively.
2) Toxicity determination was performed using the spray method: fresh kidney bean leaves are placed in a culture dish, a piece of moist filter paper is filled for moisturizing, and then two-spotted spider mites which are insects to be tested are placed in the dish, and 30 insects are placed in each dish for a contact test. Spraying the biological control medicament in the step 1) in different culture dishes of a contact test, wherein the spraying amount of each culture dish is 1mL; an 80% volume fraction ethanol solution was used as a control group and one petri dish was used as one treatment, and each treatment was repeated 3 times.
3) Processing and statistics: after treatment, the petri dishes are placed in an insect-raising room for cultivation, and the raising conditions are temperature (25+/-1) DEGC, humidity (75+/-5)%, and photoperiod 12D: and 12L, respectively counting the death number (dead without touching or feeding) after 24, 48 and 72 hours of treatment, and calculating the death rate and correcting the death rate.
The calculation formula is as follows:
Mortality = dead number/total number of insects x 100%
Corrected mortality = (treatment mortality-control mortality)/(1-control mortality) ×100% semi-neutral concentration (LC 50): refers to the concentration required to cause 50% of the subjects to die. Calculated using SPSS software, the results are shown in table 2.
Results and analysis:
TABLE 2 poisoning Activity of different biocontrol agents against Tetranychus urticae
| Biological control agent | Mortality (%) | Mortality (%) | Mortality (%) |
| Comparative example 1 | 51.9 | 58.1 | 61.5 |
| Comparative example 2 | 44.8 | 58.9 | 62.2 |
| Comparative example 3 | 24.1 | 44.8 | 51.5 |
| Example 1 | 61.9 | 82.6 | 85.9 |
As can be seen from Table 2, the contact toxicity against Tetranychus urticae was different after spraying the same concentration and dose (1 g/mL) of the biocontrol agent. The mortality rate of the two-spotted spider mites per treatment is improved along with the extension of the spraying time. Wherein, after the piperitol extract provided in example 1 is adopted, the mortality rate of the two-spotted spider mites is continuously increased along with the extension of the action time, and is at most 85.9% of 72 h; whereas the n-butanol extract of comparative example 3 had an insecticidal mortality rate of only 51.5% at 72 h. From this, it is clear that the alcohol extract of Piper hanensis has the strongest contact toxicity against Tetranychus urticae.
B. Toxicity test of different biological control agents on Tetranychus urticae
1) Preparation of the biopharmaceutical: taking 1g/mL of the mountain pepper alcohol extract, 1g/mL of the ethanol extract A, 1g/mL of the ethanol extract B and 1g/mL of the ethanol extract C prepared in example 1 and comparative examples 1 to 3 respectively, diluting by using 95% ethanol solution in volume fraction, the following experiments were conducted to obtain ethanol extracts having concentrations of 0.8g/mL, 0.6g/mL, 0.4g/mL and 0.2g/mL, ethanol extract A having concentrations of 0.8g/mL, 0.6g/mL, 0.4g/mL and 0.2g/mL, ethanol extract B having concentrations of 0.8g/mL, 0.6g/mL, 0.4g/mL and 0.2g/mL, and ethanol extract C having concentrations of 0.8g/mL, 0.6g/mL, 0.4g/mL and 0.2g/mL, and to calculate a toxicity regression equation.
2) Toxicity determination was performed using the spray method: fresh kidney bean leaves are placed in a culture dish, a piece of moist filter paper is filled for moisturizing, and then two-spotted spider mites which are insects to be tested are placed in the dish, and 10 insects are placed in each dish for a contact test. Spraying biological control agents with different concentrations in the step 1) into different culture dishes of a contact test, wherein the spraying amount of each culture dish is 1mL; with 80% ethanol as a control, one dish was treated and each treatment was repeated 3 times.
TABLE 3 determination of virulence of different biocontrol agents against Tetranychus urticae
As can be seen from Table 3, the Piper hanensis alcohol extract had the lowest LC 50 value against Tetranychus urticae, i.e. the best contact toxicity against Tetranychus urticae, compared with the other treatments.
Application example 2
A. toxicity test of frankliniella occidentalis
1) Preparation of biological control agents: the same as in step 1) of application example 1A.
2) Toxicity determination was performed using the spray method: the difference from step 2) of application example 1A is that the toxicity measurement of frankliniella occidentalis is carried out by a spraying method.
3) Processing and statistics: the results are shown in Table 4, as in step 3) of application example 1A.
Results and analysis:
TABLE 4 poisoning Activity of different biocontrol agents against Frankliniella occidentalis
As can be seen from Table 4, the same concentration and dosage (1 g/mL) of biocontrol agents were sprayed with different contact toxicity against frankliniella occidentalis. Along with the extension of the spraying time, the mortality rate of the frankliniella occidentalis treated per treatment is improved. Wherein, after using the piperitol extract provided in example 1, the highest mortality rate was 83.0% for 72 h; 10.4%, 17.8% and 13.7% higher than comparative examples 1 to 3, respectively. The mountain pepper alcohol extract has the strongest contact toxicity to frankliniella occidentalis.
B. toxicity test of different biological control agents on frankliniella occidentalis
1) Preparation of the biopharmaceutical: the same as in step 1) of B in application example 1.
2) Toxicity determination was performed using the spray method: the difference from step 2) of application example 1B is that the toxicity measurement was performed on frankliniella occidentalis by a spray method.
3) Processing and statistics: the results are shown in Table 5, as in step 3) of application example 1B.
Results and analysis:
TABLE 5 determination of toxicity of different biocontrol agents to Frankliniella occidentalis
As can be seen from table 5, the ethanol extract of mountain pepper has the lowest LC 50 value against frankliniella occidentalis, i.e., the best contact toxicity against frankliniella occidentalis, compared to other treatments.
Application example 3
A. contact toxicity test of wheat long tube aphid
1) Preparation of biological control agents: the same as in step 1) of application example 1A.
2) Toxicity determination was performed using the spray method: the difference from step 2) of application example 1A is that the virulence measurement of wheat aphid on wheat leaf is carried out by spraying.
3) Processing and statistics: the results are shown in Table 6, as in step 3) of application example 1A.
Results and analysis:
TABLE 6 poisoning Activity of different biocontrol agents against Algorithm avenae
| Biological control agent | Mortality (%) | Mortality (%) | Mortality (%) |
| Comparative example 1 | 34.7 | 62.2 | 75.9 |
| Comparative example 2 | 41.5 | 51.9 | 65.9 |
| Comparative example 3 | 41.1 | 55.6 | 72.2 |
| Example 1 | 54.8 | 72.6 | 86.3 |
As can be seen from Table 6, the contact toxicity of the biological control agent to the wheat head aphid was different after spraying the same concentration and dosage (1 g/mL). The mortality rate of the aphids per treatment of the wheat long tube is improved along with the extension of the spraying time. Wherein, after using the piperitol extract provided in example 1, the highest mortality rate was 86.3% of 72 h; 10.4%, 20.4% and 14.1% higher than comparative examples 1 to 3, respectively. The piperitol extract has the strongest contact toxicity to the wheat long tube aphid.
B. Toxicity test of different biological control agents on wheat long tube aphids
1) Preparation of the biopharmaceutical: the same as in step 1) of B in application example 1.
2) Toxicity determination was performed using the spray method: the difference from step 2) of application example 1B is that the virulence measurement of wheat aphid on wheat leaf is carried out by spraying method.
3) Processing and statistics: the results are shown in Table 7, as in step 3) of application example 1B.
Results and analysis
TABLE 7 determination of virulence of different biocontrol agents against Metarhizium griseum
As can be seen from table 7, the LC 50 value of the kaempferol extract was the lowest for the long tube aphid, i.e. it was the best contact poisoning power for the long tube aphid, compared to the other treatments.
Application example 4
A. Contact toxicity test of Asiatic corn borer
1) Preparation of biological control agents: the same as in step 1) of application example 1A.
2) Toxicity determination was performed using the spray method: the difference from step 2) of application example 1A is that the toxicity measurement of Asian corn borer on corn leaves is carried out by a spraying method.
3) Processing and statistics: the results are shown in Table 8, as in step 3) of application example 1A.
Results and analysis:
TABLE 8 poisoning Activity of different biocontrol agents against Asiatic corn borer
| Biological control agent | Mortality (%) | Mortality (%) | Mortality (%) |
| Comparative example 1 | 51.9 | 58.9 | 62.2 |
| Comparative example 2 | 51.9 | 55.6 | 58.9 |
| Comparative example 3 | 44.8 | 51.9 | 55.2 |
| Example 1 | 58.9 | 69.0 | 75.9 |
As can be seen from Table 8, the biocontrol agents at the same concentration and dosage (1 g/mL) have different contact toxicity to corn borers after spraying. The mortality rate per treatment of Asian corn borers increases with the spray time. Wherein, after using the piperitol extract provided in example 1, the highest mortality rate was 75.9% of 72 h; while the highest mortality rates for comparative examples 1-3 were only 62.2%, 58.9% and 55.2%. It can be seen that the ethanol extract of mountain pepper has the strongest contact toxicity to Asiatic corn borers.
B. Toxicity test of different biological control agents on wheat long tube aphids
1) Preparation of the biopharmaceutical: the same as in step 1) of B in application example 1.
2) Toxicity determination was performed using the spray method: the difference from step 2) of application example 1, B, is that the toxicity measurement of Asian corn borer on corn leaves was performed by a spraying method.
3) Processing and statistics: the results are shown in Table 9, as in step 3) of application example 1B.
Results and analysis:
TABLE 9 determination of virulence of different biocontrol agents against Asiatic corn borer
As can be seen from table 9, the kaempferol extract had the lowest LC 50 value for asian corn borers, i.e., its contact toxicity to asian corn borers, compared to the other treatments.
In summary, it is known that the katsurada alcohol extract can be used to kill both the wheat head aphid, the spider mite, the frankliniella occidentalis and the Asiatic corn borer. In the killing process, the killing time is positively related to the death rate of pests; the concentration of the katsumadai alcohol extract is also positively correlated with mortality of the pests. Experiments prove that the mortality rate of killing wheat long tube aphids, two-spotted spider mites, frankliniella occidentalis and Asiatic corn borers is up to 86.7 percent by adopting the technical scheme provided by the invention, and the broad-spectrum pesticide composition is strong in broad-spectrum property, good in control effect and green and safe.
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.
Claims (7)
1. Use of an alcohol extract of mountain pepper (Lindera glauca) for combating agricultural pests and/or for the preparation of a biological control product for combating agricultural pests, characterized in that said species of agricultural pests comprise: one or more of Long tube aphids, two-spotted spider mites, frankliniella occidentalis and Asiatic corn borers;
the preparation method of the mountain pepper alcohol extract comprises the following steps:
mixing a mountain pepper sample with alcohol, and performing first ultrasonic extraction to obtain a first leaching solution and first filter residues respectively;
Mixing the first filter residue with alcohol again for second ultrasonic extraction to obtain a second leaching solution;
Mixing the first leaching solution and the second leaching solution, and concentrating the mixture into a pasty substance to obtain the mountain pepper alcohol extract;
the alcohol is an ethanol solution with the volume percentage of 95 percent.
2. The use according to claim 1, wherein the mountain pepper sample is selected from one or more of the root, stem, leaf and fruit of mountain pepper plant.
3. The use according to claim 1, wherein the weight to volume ratio of the mountain pepper sample to the ethanol solution is 0.5-1.5 g: 10-40 mL.
4. Use according to claim 1 or 3, characterized in that the weight to volume ratio of the mountain pepper sample to the ethanol solution is 1g:10mL.
5. The use according to claim 1, wherein the time of the first ultrasound and the second ultrasound is 20-50 min, respectively.
6. The use according to claim 1 or 5, wherein the time of the first ultrasound and the second ultrasound is 30min, respectively.
7. The use according to claim 1, wherein the alcohol extract of mountain pepper is dissolved by alcohol ultrasonic wave to obtain mountain pepper alcohol extract when spraying insects;
the concentration of the mountain pepper alcohol extract is 0.2-1 g/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311397223.1A CN117441746B (en) | 2023-10-26 | Application of mountain pepper alcohol extract in killing agricultural pests and/or preparing broad-spectrum biological control product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311397223.1A CN117441746B (en) | 2023-10-26 | Application of mountain pepper alcohol extract in killing agricultural pests and/or preparing broad-spectrum biological control product |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117441746A CN117441746A (en) | 2024-01-26 |
| CN117441746B true CN117441746B (en) | 2024-06-28 |
Family
ID=
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090044100A (en) * | 2007-10-31 | 2009-05-07 | 주식회사 코리아나화장품 | Anti-aging cosmetic composition comprising the extract of lindera glauca as active ingredient |
| CN114762505A (en) * | 2022-03-25 | 2022-07-19 | 西北农林科技大学 | Wild pepper nematicide, preparation method and application thereof |
| CN115606797A (en) * | 2021-07-12 | 2023-01-17 | 芙蓝宝株式会社 | Composition for promoting hair growth or inhibiting alopecia comprising water-soluble mastic gum and fructus Piperis extract |
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090044100A (en) * | 2007-10-31 | 2009-05-07 | 주식회사 코리아나화장품 | Anti-aging cosmetic composition comprising the extract of lindera glauca as active ingredient |
| CN115606797A (en) * | 2021-07-12 | 2023-01-17 | 芙蓝宝株式会社 | Composition for promoting hair growth or inhibiting alopecia comprising water-soluble mastic gum and fructus Piperis extract |
| CN114762505A (en) * | 2022-03-25 | 2022-07-19 | 西北农林科技大学 | Wild pepper nematicide, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101851356B (en) | Shitake mushroom extract and application thereof | |
| Hussain et al. | Determination of pesticides residues in selected varieties of mango | |
| CN1947522A (en) | Botany resource disinfectants for agricultural use an dits prepn. method | |
| CN102177921B (en) | Compound biological control agent as well as preparation method and application thereof | |
| CN105248489B (en) | A kind of biological agent for tea place preventing and treating pest and disease damage and preparation method thereof | |
| CN101297648A (en) | Bactericide agent composition containing mushroom proteoglycan | |
| CN103444729A (en) | Bactericidal pharmaceutical composition and applications thereof | |
| CN101233867A (en) | Matrine agent plant pesticide and preparation thereof | |
| CN104920487B (en) | A kind of Chinese medicinal material extract and preparation method thereof and the method using Chinese medicinal material extract preventing and treating wheat aphid | |
| JPWO2003020032A1 (en) | Composition for inducing plant disease resistance and method for producing the same | |
| Asran et al. | Pathogenicity of Fusarium graminearum isolates on maize (Zea mays L.) cultivars and relation with deoxynivalenol and ergosterol contents/Pathogenität von Fusarium graminearum Isolaten an Mais-(Zea mays L.) Sorten und Beziehung zu Deoxynivalenol-und Ergosterol-Gehalten | |
| CN105918355A (en) | Special green muscardine fungus insecticide for preventing and treating Pleonomus canaliculatus, and preparation method thereof | |
| CN117441746B (en) | Application of mountain pepper alcohol extract in killing agricultural pests and/or preparing broad-spectrum biological control product | |
| CN115644181B (en) | Chrysanthemum white rust control agent and use method thereof | |
| CN115211427B (en) | Insecticidal composition containing mesoionic insecticide and application thereof | |
| CN117441746A (en) | Application of mountain pepper alcohol extract in killing agricultural pests and/or preparing broad-spectrum biological control product | |
| Dharumarajan et al. | Persistence of combination-mix (β-cyfluthrin+ imidacloprid) on tomato (Lycopersicon esculentum) | |
| CN109832300B (en) | Pesticide composition for preventing and treating plant virus diseases and preparation method and application thereof | |
| CN105638662B (en) | Curculigoside is applied in insecticide is prepared | |
| CN113796392B (en) | Fungicide of derris elliptica extract and preparation method and application thereof | |
| CN110839652A (en) | Compound pesticide of Isaria javanicus mutant strain IJUV-6 and dinotefuran and application thereof | |
| CN114403146B (en) | Application of pullulan in preparation of plant resistance inducer, plant resistance inducer and method | |
| CN109315424A (en) | Biogas slurry biological pesticide and preparation method thereof | |
| CN115005241B (en) | Botanical pesticide, preparation method and application thereof | |
| CN114208855B (en) | Botanical bactericidal composition for preventing and treating rice sheath blight disease, preparation and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant |