KR20090044100A - Anti-aging cosmetic composition comprising the extract of lindera glauca as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing skin aging containing persimmon extract as an active ingredient, and in detail, the cosmetic composition of the present invention is 0.0001 to 30% by weight based on the total dry weight of the persimmon extract as an active ingredient It is characterized by containing.

The persimmon extract of the present invention is excellent in antioxidant activity to eliminate free radicals, excellent anti-wrinkle effect, and excellent stability when formulated into cosmetics and has an excellent anti-aging effect.

Persimmon Tree, Free Radical, Antioxidant, Anti-Aging

Description

Anti-Aging Cosmetic Composition Comprising the Extract of Lindera glauca as Active Ingredient}

 The present invention relates to a cosmetic composition excellent in antioxidant effect, anti-aging containing persimmon extract as an active ingredient.

      Cosmetics are deeply involved in our daily lives and used by many people. Before the development of human civilization, the purpose of cremation was to protect the body from nature.However, with the development of science and civilization, the purpose of cremation is to keep the body clean and beautiful, and to delay the aging to make a beautiful and pleasant life. Changed.

Cosmetic functions include not only clean and aesthetic functions, but also free radicals due to internal factors such as deterioration of immune cells due to external factors such as moisture supply, pollutants, stress and ultraviolet rays, and deterioration of cell activity. This is to remove or suppress the cause of skin aging such as destruction of proteins, nucleic acids and cell membrane lipids caused by free radicals and peroxides. In addition, cosmetics having whitening, skin wrinkle improvement and suppression, and sunscreen functions to meet the consumer's desire for high-performance cosmetics continue to be developed. In particular, wrinkle improvement and suppression products are of great interest to consumers seeking to stay younger.

In general, as the skin ages, the skin loses its moisture, elasticity, color, and glossiness due to internal and external circumstances. In particular, as cells age, their ability to eliminate their own free radicals is weakened. These free radicals move around the body, causing damage to cell membranes and attacking the DNA sequences in the cell nucleus, causing genetic mutations to promote aging. .

In order to prevent damage to skin cells caused by these free radicals, a free radical of the skin is applied to the skin with a substance having excellent antioxidant capacity. This can prevent or delay skin aging.

Materials known to have free radical scavenging ability to date include ascorbic acid, DL-alpha-tocopherol, beta-carotene dibutylhydroxytoluene (BHT), and the like.

However, these substances have a problem of its own stability and strong skin irritation, so it is too difficult to apply to cosmetics. Therefore, the development of new antioxidant cosmetics that can be applied to the skin more safely and usefully is required.

      The present inventors have overcome the problems with the conventional free radical scavenging ability of stability, safety, and the like, and to develop a material having a superior antioxidant effect, it is frequently used in herbal medicine or folk medicine to prove the safety of natural products During the intensive study of the subject, it was found that the free radical scavenging ability of the persimmon tree extract was excellent and the present invention was completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition for preventing skin aging comprising the extract of persimmon.

The present invention provides a cosmetic composition for preventing skin aging containing the extract of persimmon as an active ingredient.

Lindera glauca is a small tall tree of the camphor tree. It grows in sunny places in the south of central Korea. It grows about 5 to 8 meters in height and grows to 5 to 13 centimeters in diameter. The bark is smooth and off-white.

Leaves are shiny and shiny on the back, grayish brown, petiole 6 to 10 mm long. Buds sprout in scarlet colors and fall leaves turn scarlet or orange. It is characterized by dried dead leaves attached to the branches until the sprouting of the next year. It is a male and female tree, with light yellow small flowers blooming between the leaves in April. In September, the round fruit, about 8 mm in diameter, ripens.

Fruits, leaves, stems, and roots are all used for medicinal purposes. Fruits are called coral reefs. Root has the effect of eliminating customs, shattering blood, and communicating meridians.It cures numbness, joint pain and muscle pain, cold and sore symptoms, and bruises caused by customs.

Persimmon tree extract contained in the cosmetic of the present invention is extracted by a conventional extraction method from the roots, stems, leaves or bark of various parts of the persimmon tree.

The persimmon extract used in the present invention may be obtained through various methods known in the art, and preferably water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, chloroform, butyl acetate or 1, 3-butylene glycol can be used as an extraction solvent. Preferably it is obtained using alcohol as the extraction solvent.

Method of producing persimmon extract contained in the cosmetic of the present invention is as follows. First wash the whole Ego tree (roots, stems, leaves) with purified water, and then dry and crush it into small pieces, which is 1-10 times the dry weight of water, anhydrous or hydrous lower alcohol, ethyl Acetate, chloroform, butyl acetate or 1,3-butylene glycol are added. Thereafter, a cooling condenser is installed to extract 1-15 days at room temperature while preventing the active ingredient from evaporating, and is dried by a rotary vacuum evaporator to prepare it. In this case, in the case of 1-3 butylene glycol, it is difficult to dry using a rotary vacuum evaporator, and then directly extracted from the above conditions, and then used to adjust the drying loss to 1% (w / v) in the cosmetic of the present invention.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent, but also extract of Ecklonia chinensis which exhibits substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-mentioned extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the persimmon extract of the present invention.

The persimmon extract of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure, freeze drying or spray drying.

The amount of persimmon extract added to the cosmetic of the present invention is 0.0001 to 30% by weight, preferably 0.01 to 10% by weight and most preferably 0.1 to 5.0% based on the total weight of the cosmetic composition. If it is less than 0.0001%, it is difficult to expect a clear effect, and if it is more than 30.0%, it does not have a special advantage as the content increases.

According to a preferred embodiment of the present invention, the persimmon extract has excellent free radical scavenging activity (Experimental Example 1), excellent inhibitory effect on hydrogen peroxide-induced cytotoxicity (Experimental Example 2), almost no cytotoxicity (Experimental Example 3) ), Shows an excellent antioxidant effect. The cosmetic composition of the present invention can effectively prevent skin aging through such excellent antioxidant effect of persimmon extract.

When the composition of the present invention is formulated as a cosmetic, the components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the extract of persimmon as an active ingredient, such as antioxidants, stabilizers, solubilizers, Conventional adjuvants such as vitamins, pigments and flavorings, and carriers.

The anti-aging cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants -Can be formulated with, but not limited to, cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like. More specifically, it may be prepared in the form of softening water, converging cream, nutrient cream (lotion), nutrition cream, massage cream, essence, pack, oil-in-water type (O / W) or water-in-oil type (W / O). .

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

As used herein, the term 'anti-aging of the skin' has a meaning encompassing comprehensively the prevention and treatment of skin aging. Therefore, the anti-aging of the skin, which is the use of the present invention, is interpreted to include the improvement of wrinkles, the moisturizing ability, the skin protection by the antioxidant effect, and the improvement of skin elasticity.

In addition, the cosmetic composition of the present invention does not have problems such as skin irritation induced or instability of the formulation appearing in the existing anti-aging cosmetic composition.

The cosmetic composition of the present invention containing persimmon extract extracts the free radicals of the skin, thereby inhibiting oxidation of the biological material by the free radicals, thereby preventing or delaying aging of the skin.

 Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Example  One : Persimmon tree  Preparation of Extract

After washing with purified water and drying, 1kg of the pulverized persimmon fine powder was put in 3L of water, and immersed at room temperature for 15 days, and then filtered with a filter paper of Whatman 2 times. The filtrate was concentrated in a vacuum condenser with a cooling capacitor and lyophilized to give a dry weight of 58 g of persimmon tree.

Examples 2-15: Persimmon tree  Preparation of Extract

Example Extraction solvent Weight (g) of persimmon tree extract from 1 kg of sample powder Example 2  25% ethanol  43 Example 3  50% ethanol  52 Example 4  75% ethanol  67 Example 5  100% ethanol  54 Example 6  80% methanol  52 Example 7  100% methanol  49 Example 8  Acetone  34 Example 9  Diethyl ether  21 Example 10  chloroform  15 Example 11  Hexane  8 Example 12  Normal-propanol  23 Example 13  Isopropanol  21 Example 14  Normal-butanol  15 Example 15  Ethyl acetate  10

Experimental Example  One : Persimmon tree  Of extract Free radical  Scavenging test

Extracts of Ecklonia cava extracts of Examples 1 to 15, ascorbic acid (ascorbic acid), DL-alpha-tocopherol, and beta-carotene dibutylhydroxyltoluene (BHT) A radical scavenging test was performed. The test method is as follows.

That is, 2 μl of 60 μM 1,1-diphenyl-2-picryl hydrazyl (1,1-diphenyl-2-picryl hydrazyl, DPPH) ethanol solution and persimmon extract in a dimethyl sulfoxide (DMSO) solution at a 1: 100 ratio 2 ml of the diluted solution was added thereto, reacted at room temperature for 30 minutes, and the absorbance was measured at 520 nm. Instead of the persimmon extract, only DMSO solution was used as a control. Ascorbic acid, DL-alpha-tocopherol, beta-carotene, and dibutyl hydroxyltoluene used in the comparative group were used at a concentration of (1%).

The free radical scavenging activity (%) was calculated by the following formula.

                                   Absorbance at Extract Treatment

 Free radical scavenging effect (%) = 100-(----------------------- X 100)

                                      Control absorbance

The results are as shown in Table 2 below.

Free Radical Scavenging Activity of Extracts from Persimmon Tree and Existing Antioxidants Test substance Free radical scavenging rate (%)  Example 1 80.0  Example 2 78.4  Example 3 89.6  Example 4 88.5  Example 5 74.7  Example 6 64.9  Example 7 57.8  Example 8 50.5  Example 9 30.1  Example 10 41.0  Example 11 25.9  Example 12 88.8  Example 13 86.7  Example 14 28.1  Example 15 45.2 Ascorbic acid 91.2 DL-Al-Tocopherol 87.3 Beta-carotene 87.5 Dihydroxytoluene (BHT) 90.1

Through the above results, the extracts of persimmon tree of the present invention except the fat-soluble Examples 9-11 and 14 exhibited a high activity comparable to other antioxidants, and this activity shows that the persimmon tree extract has a strong antioxidant activity as a natural product. It can be seen that

Experimental Example  2 : Persimmon tree  Inhibitory Effect of Extracts on Hydrogen Peroxide-induced Cytotoxicity

The inhibitory effect on hydrogen peroxide-induced cytotoxicity of the persimmon tree extract prepared in Example 4 was measured using mouse-derived fibroblasts.

Hydrogen peroxide produces hydroxyl radicals in cells, causing lipid peroxidation, leading to cell membrane damage and cytotoxicity. Free radical scavenging activity of E. coli extract was measured as follows to test at the skin cell level.

Inoculate mouse skin fibroblasts of logarithmic growth stage into a 96 well plate at 10,000 cells per well and incubate in 80 µl of DMEM medium for 24 hours, and then dilute the media to a final concentration of 10 ^-3 M in the medium. 10 μl of hydrogen peroxide was added thereto, and 10 μl of the diluted diluted persimmon tree extract of Example 4 was added to the dimethyl sulfoxide solution to a final concentration of 0, 200, and 500 g / ml. After 4 hours of 15 ml / ml of MTT solution was added and 150 µl of dimethyl sulfoxide solution was dissolved per well, the inhibitory effect of persimmon extract on hydrogen peroxide-induced cytotoxicity was calculated by the following formula. .

               A-B

Cytotoxic inhibitory effect (%) = ------------ X 100

               B

A: absorbance when extract treatment, B: absorbance when hydrogen peroxide alone treatment

The results are as shown in Table 3 below.

Inhibitory Effect of Persimmon Tree Extract on Hydrogen Peroxide-induced Cytotoxicity Concentration of Persimmon Tree Extract (ug / ml) Cytotoxic effect (%) 0 4.2 200 30.4 500 60.3

Through the above results, it was shown that the persimmon extract was concentration-dependently inhibited against hydrogen peroxide-induced cytotoxicity. Therefore, it can be seen that the E. coli extract inhibits cytotoxicity by inhibiting lipid peroxidation produced in mouse-derived fibroblasts.

Experimental Example  3: cytotoxicity experiment

Cytotoxicity of the persimmon extract itself prepared in Example 4 was measured using the mouse-derived dermal fibroblasts as follows.

Mouse skin fibroblasts of logarithmic growth stage were inoculated in a 96 well plate at 10,000 per well and incubated in 80 μl of DMEM medium for 24 hours, and then the persimmon extract of Example 4 was prepared at 0, 50, 100, 200 10 μl of the diluted solution prepared by diluting with dimethyl sulfoxide to 500 g / ml was added thereto, and further incubated for 20 hours, and then 15 μl of 5 mg / ml MTT solution was added thereto, followed by 4 hours of dimethyl sulfoxide solution. 150 μl per well was added to dissolve and the absorbance was measured at 570 nm with a microplate reader.

Cytotoxicity of persimmon extract itself was expressed as cell viability (%), and was calculated by the following formula.

         Absorbance at Extract Treatment

Cell survival rate (%) = ---------------------- X 100

        Control absorbance

The results are as shown in Table 4 below.

Cytotoxicity of Persimmon Extract Treatment Concentration of Persimmon Extract (ug / ml) Cell survival rate (%) 0 100 50 101.2 100 100.3 200 104.1 500 102.5

Through the results of Table 4, the persimmon extract of Example 4 did not show a significant cytotoxicity as compared to the negative control as it is.

Prescription Example  1: flexible cosmetics

Prescription examples of flexible cosmetics in cosmetics containing persimmon extract are as follows. Here, Ecklonia cava extract refers to that of Example 4.

ingredient Content (unit: weight%) Persimmon Tree Extract 0.1 glycerin 5.0 1,3-butylene glycol 3.0 Fiji 1500 1.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na (EDTA-2Na) 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 ethanol 10.0 Oltildodeceth-16 0.2 Polysorbate 20 0.2 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example  2: convergence makeup

Prescription examples of astringent makeup among cosmetics containing persimmon extract are as follows. Here, Ecklonia cava extract refers to that of Example 4.

ingredient Content (unit: weight%) Persimmon Tree Extract 0.1 glycerin 2.0 1,3-butylene glycol 2.0 Fiji 1500 1.0 Allantoin 0.2 DL-panthenol 0.2 Sodium hyaluronate 3.0 ethanol 15.0 Octyldodeceth-16 0.2 Polysorbate 20 0.3 Witch hazel extract 2.0 Citric acid a very small amount antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example  3: Nutritional Cosmetics

Prescription examples of nutrient cosmetic lotion (lotion) in cosmetics containing persimmon extract are as follows. Here, Ecklonia cava extract refers to that of Example 4.

ingredient Content (unit: weight%) Persimmon Tree Extract 1.0 glycerin 5.0 1,3-butylene glycol 3.0 Sodium Hiharuronate 5.0 ethanol 10.0 Polysorbate 60 1.3 Glyceryl Stearate S 1.5 Cetearyl Alcohol 1.5 lanolin 1.5 Sorbitan stearate 0.5 Cured Vegetable Oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 2.0 Dimethicone 0.8 Tocopherol Acetate 0.5 Carboxy Vinyl Polymer 0.12 Triethanolamine 0.12 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example  4: nutrition cream

Prescription example of nutrition cream among cosmetics containing persimmon extract is as follows. Here, Ecklonia cava extract refers to that of Example 4.

ingredient Content (unit: weight%) Persimmon Tree Extract 2.0 glycerin 5.0 Sodium hyaluronate 4.0 Folisysorbate 60 1.5 Cured Vegetable Oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoine 5.0 Dimethicone 1.0 Triethanolamine 1.0 Lipophilic Monostearic Acid Glycerin 2.0 Cetearyl Alcohol 2.2 Stearic acid 1.5 Beeswax 1.0 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example  5: essence

Prescription examples of the essence of the cosmetic containing the extract of Echinacea are as follows. Here, Ecklonia cava extract refers to that of Example 4.

ingredient Content (unit: weight%) Persimmon Tree Extract 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Prescription Example  6: pack

Prescription examples of packs containing cosmetic extracts are as follows. Here, Ecklonia cava extract refers to that of Example 4.

ingredient Content (unit: weight%) Persimmon Tree Extract 3.0 glycerin 3.0 Fiji 1500 2.0 Allantoin 0.1 DL-panthenol 0.4 ethanol 6.0 Cyclodextrin 0.15 Monomethylammonium Glycyric Acid 0.3 Nicotinamide 0.5 Fiji 40 Cured Castor Oil 0.3 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Claims (4)

Lindera glauca ) Cosmetic composition for preventing skin aging containing the extract. The cosmetic composition for preventing skin aging according to claim 1, wherein the extract of persimmon tree is contained in an amount of 0.0001-30% by weight based on the total weight of the cosmetic composition. The method of claim 1, wherein the extract of persimmon tree using a solvent selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene glycol Skin aging cosmetic composition, characterized in that extracted by using a supercritical, ultrasonic extraction method. The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition is a softening cream, astringent makeup, nutrient cream (lotion), nutrition cream, massage cream, essence, pack, oil-in-water (O / W) or water-in-oil type ( Cosmetic composition for preventing skin aging, characterized in that the formulation of W / O).