KR102303414B1 - Cosmetic Composition for Skin Whitening Containing Glehnia littoralis Extracts or Fraction thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening comprising a fraction of a Gaetbangpung extract or a supercritical extract of Gaetbangpung extract, and by applying hexane when preparing a fraction of Gaetbangpung extract, the hexane fraction of the Gaetbangpung extract obtained by using a different solvent Compared to the case of application, the melanin synthesis inhibitory effect is excellent, and the Gaebangpung supercritical extract obtained by supercritical extraction of Gaetbangpung has excellent melanin synthesis inhibitory effect. Therefore, the fraction of the Gaetbangpung extract or the Gaetbangpung supercritical extract of the present invention can be usefully used in the cosmetic manufacturing field requiring a whitening effect.

Description

A cosmetic composition for skin whitening comprising Gaetbangpung extract or a fraction thereof {Cosmetic Composition for Skin Whitening Containing Glehnia littoralis Extracts or Fraction thereof}

The present invention relates to a cosmetic composition for skin whitening comprising an extract of Gaetbangpung or a fraction thereof.

One of the cosmetic fields that women are most interested in is skin whitening. 'Skin whitening' refers to a change in skin color that occurs due to excessive production of pigment in the skin due to environmental changes such as ultraviolet rays, stress, abnormal hormone secretion, and pregnancy. The most important factor in this regard is melanin, the skin pigment. Melanin is synthesized in melanocytes, which are pigment cells in the basal layer of the epidermis, and metastasizes to surrounding keratinocytes to determine skin color. Melanin is a uniquely stable pigment found in living organisms, and there are many unknowns about the chemical properties of melanin, and the progress of research is also sluggish.

Hyperpigmentation of the skin may be caused by several factors such as hormonal abnormalities, genetic diseases, and ultraviolet irradiation after the inflammatory reaction of the skin, the main factors being due to abnormalities in the synthesis and distribution of melanin pigments. The main function of melanin is to protect the skin from damage by removing oxygen radicals. The abundance of melanin means that it has an effective countermeasure system to protect the skin from physical and chemical toxic substances.

However, excessive deposition of melanin also causes pathological problems such as skin, and is recognized as a cosmetic problem such as spots, freckles, spots, and age spots. Therefore, interest in whitening agents capable of inhibiting melanin formation is increasing and many studies are being conducted. Conventionally, various whitening cosmetics have been proposed for the purpose of preventing pigmentation on the skin. For example, vitamin C and its derivatives (Patent Document 1), reducing agents such as glutathione, hydrogen peroxide, placental extract, and melanin production inhibitory substances such as kojic acid are known.

However, vitamin C is easily oxidized, and there are disadvantages in that cosmetics containing vitamin C are discolored and odorless. A reducing agent such as glutathione not only has an unpleasant odor, but also has a problem in transdermal absorption, and hydrogen peroxide has a problem in safety and stability. In addition, animal tissue extraction systems such as placental extracts do not have sufficient effects, and also have problems in terms of stability, color, odor, and the like. Moreover, since kojic acid also has a problem that the effect of preventing darkening of the skin is small with a small amount, it is difficult to say that all of them are sufficient as an active ingredient of a whitening cosmetic.

The above-mentioned melanin production inhibitory substances are insufficient in effect or practical in various aspects such as safety and stability when only these are used.

Korean Patent Registration No. 1639615

An object of the present invention is to provide a material for skin whitening that has excellent whitening effect, has no side effects and has high safety.

In order to solve the above problems, the present invention provides a cosmetic composition for skin whitening comprising a fraction obtained by fractionating a Gaetbangpung extract with an organic solvent.

In addition, the present invention provides a cosmetic composition for skin whitening comprising a gaetbangpung supercritical extract.

The fraction of the Gaetbangpung extract or the Gaetbangpung supercritical extract of the present invention inhibits melanin synthesis to a level similar to that of the positive control at a lower concentration than the positive control p-coumaric acid or arbutin, respectively, so it can be used for skin whitening.

1 is a schematic diagram showing a method for preparing a fraction of an ethanol extract of Gaetbangpung of the present invention.
Figure 2 shows the cumulative extraction amount according to the extraction time of the Gaetbangpung supercritical extract.
Figure 3 shows the melanin synthesis inhibition evaluation result of the hexane fraction of Gaetbangpung ethanol extract of the present invention; p-coumaric acid: positive control, n-Hexane: hexane fraction of Gaetbangpung ethanol extract, n-Hexane water layer: fraction obtained by fractionating Gaetbangpung ethanol extract with hexane and only the water layer, MC: methylene of Gaetbangpung ethanol extract Chloride fraction, EthylAcetate: Ethyl acetate fraction of Gaetbangpung ethanol extract, n-Butanol: Butanol fraction of Gaetbangpung ethanol extract, n-Butanol Water layer: Fractionation of Gaetbangpung ethanol extract with butanol, followed by separation of only the water layer.
4 shows the results of evaluation of inhibition of melanin synthesis of Gaetbangpung supercritical extract.

Hereinafter, the present invention will be described in detail.

1. Preparation of fractions of Gaetbangpung extract

Gaetbangpung ( Glehnia littoralis ) is a perennial herb that grows in sand. They live attached to sand or coastal cliffs in sunny areas. The height is 5-20 cm, and the leaves are 10-20 cm long. Roots are yellow, thick, and extend vertically into the ground, and are called empty windmills and are used for medicinal purposes, and petioles are used for food.

The extract of the present invention contains leaves, young shoots, main body, main body shells, stems, stem shells, roots, root shells, rhizomes, seeds, fruits, immature fruits, mature fruits, pulp, pericarp, flowers, stamens ( androecium), pistils (gynoecium), sepals, stamens, petals, sepal pieces, carpels, and combinations thereof. The rhizome is also referred to as a rhizome, indicating that the stem grows underground and acts like a root. The stamen group represents the entire stamen in one flower, and the pistil group represents the entire pistil in one flower. The carpel is a component that makes the pistil of a flower and represents a deformed form of a leaf, which is generally called a flower leaf.

The present invention provides a cosmetic composition comprising a fraction of the Gaetbangpung extract comprising; preparing a fraction by fractionating the Gaetbangpung extract with an organic solvent.

As used herein, the term "extract" refers to a material extracted from a raw material by any method, and is used in the meaning of including, without limitation, the extracted extract, the concentrate obtainable therefrom, the dried product and the powder of the concentrate.

The extract may be obtained by extraction from a raw material or a dried product thereof, and the raw material of the extract may be used without limitation, such as cultivated or commercially available ones.

When the extract is obtained by extracting it from the raw material, as the extraction method, all conventional known extraction methods such as solvent extraction, ultrasonic extraction, filtration, and reflux extraction can be used, and it can be prepared by using a solvent extraction method or a reflux extraction method. . The extraction process may be repeated several times, and thereafter, a step such as concentration or freeze-drying may be additionally performed. Specifically, the obtained extract is concentrated under reduced pressure to obtain a concentrate, and after freeze-drying the concentrate, a high concentration extract powder can be prepared by using a grinder. The extract also includes a fraction obtained by further fractionating the extract.

The extract may be extracted using water, an organic solvent, or a mixture thereof as an extraction solvent. The organic solvent is alcohol, C ₁ -C ₄ lower alcohol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene and these It may be any one selected from the group consisting of a mixed solvent, and may be ethanol. When a mixture of water and an organic solvent is used as the extraction solvent, the mixture of water and organic solvent may be a mixture of water and a C ₁ -C ₄ lower alcohol, or a mixture of water and ethanol.

The mixture of water and ethanol is 40% (v/v) to 90% (v/v) aqueous ethanol solution, 45% (v/v) to 85% (v/v) aqueous ethanol solution, 50% (v/v) to 80% (v/v) aqueous ethanol solution, 55% (v/v) to 75% (v/v) aqueous ethanol solution, or 60% (v/v) to 75% (v/v) ethanol aqueous solution , but not limited thereto. When the concentration of the aqueous ethanol solution is less than the lower limit, the whitening effect does not appear even if the extract is fractionated. When the concentration of the aqueous ethanol solution exceeds the upper limit, the stability of the formulation particles is lowered when included in the cosmetic composition, and the whitening effect is lowered. In addition, when the concentration of the aqueous ethanol solution exceeds the upper limit, the fraction contains a high concentration of ethanol, and when the fraction is added to the cosmetic composition without a separate ethanol removal process, skin irritation and toxicity can be caused, and the stability of physical properties is lowered may cause In order to solve this problem, when ethanol is removed through a separate process, the whitening efficacy of the fraction is reduced, and the raw material production cost is increased by adding the ethanol removal process.

The extraction may be carried out at 0 °C to 100 °C, 10 °C to 70 °C, 10 °C to 50 °C, 10 °C to 35 °C, or 20 °C to 35 °C, but is not limited thereto. The extraction may be carried out for 12 hours to 36 hours, 12 hours to 30 hours, 15 hours to 30 hours, 17 hours to 25 hours, or 20 hours to 25 hours, but is not limited thereto. The extraction may be performed 1 to 8 times, may be performed 1 to 6 times, and may be performed 1 to 5 times, but is not limited thereto, and the extract may be a single extract obtained from each extraction or obtained from each extraction. It may be a mixed extract of extracts. Among the extraction conditions, when the extraction temperature and time are less than the lower limit or the number of extractions exceeds the upper limit, the whitening effect does not appear even if the Gaetbangpung extract is fractionated with hexane.

The organic solvent used in preparing the fraction may be, for example, C ₅ to C ₁₀ alkane, hexane, or the like, and the organic solvent used in preparing the fraction may be hexane.

When fractionating the ethanol extract of Gaetbangpung with hexane, with respect to 1g of the powder of Gaetbangpung extract, hexane is 10㎖ to 300㎖, 10㎖ to 250㎖, 20㎖ to 200㎖, 30㎖ to 100㎖, 30㎖ to 80㎖ may be used, but is not limited thereto. When hexane is treated below the lower limit, the active ingredient may not be sufficiently fractionated, and when hexane is treated in excess of the upper limit, a large amount of time and money is consumed to evaporate the hexane solvent from the hexane fraction, and environmental pollution problems There is this.

The fraction may be prepared at 0 °C to 100 °C, 2 °C to 90 °C, 5 °C to 80 °C, 10 °C to 70 °C, 10 °C to 60 °C, or room temperature. Room temperature represents a temperature of about 10° C. to about 35° C. without a separate device for temperature control. If the temperature is less than the lower limit, the solute from the Gaetbangpung ethanol extract to hexane is not sufficiently dissolved into hexane even if the solute is highly soluble in hexane. does not When the temperature exceeds the upper limit, hexane is decomposed to generate a toxic gas, and there is a problem in that the physical properties are changed, and the whitening effect does not appear.

When preparing the fraction, the settling time after the organic solvent treatment may be 12 hours to 36 hours, 12 hours to 30 hours, 15 hours to 30 hours, 17 hours to 25 hours, or 20 hours to 25 hours, but is not limited thereto. When the settling time is less than the lower limit, the whitening effect of the fraction is reduced.

In one embodiment of the present invention, 70% (v/v) aqueous ethanol solution was added to Gaetbangpung, extracted for 24 hours, and the resulting extract was concentrated under reduced pressure and dried. After mixing, the mixture was allowed to stand at room temperature for 24 hours. Only the hexane layer was separated from the solution separated into the hexane layer and the water layer. The whitening effect of the hexane fraction was evaluated, and treatment at a concentration of 12.5 μg/ml was found to inhibit melanin synthesis at a level similar to that of the positive control.

The hexane fraction of the Gaetbangpung extract may be additionally subjected to a conventional purification process in order to enhance the effect of improving whitening, and the purified product obtained through the additional purification process is also included in the present invention. Purified product obtained by passing the hexane fraction according to the present invention through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. Active fractions obtained through various purification methods carried out are also included in the fractions of the present invention. A specific active fraction having a higher whitening improvement effect can be prepared by separating the fraction in which several components are mixed according to the properties of the active ingredient through gradient column chromatography or the like.

The column chromatography can separate and purify the active fraction using a filler selected from the group consisting of silica gel, Sephadex, LH-20, ODS gel, RP-18, polyamide, Toyopearl and XAD resin. . Column chromatography may be performed several times by selecting an appropriate filler as necessary, but is not limited thereto. In using the chromatography, the elution solvent, elution rate, and elution time may be applied to a solvent, rate, or time generally used in the art.

2. Preparation of Gaetbangpung Supercritical Extract

The present invention provides a cosmetic composition for skin whitening comprising a Gaetbangpung supercritical extract obtained by supercritical extraction of Gaetbangpung.

Gaetbangpung, the extraction site of Gaetbangpung, and the definition of the extract are the same as those described in "1. Preparation of the fraction of the Gaetbangpung extract" , so the description is omitted.

The extract may be obtained by extraction from a raw material or a dried product thereof, and the raw material of the extract may be used without limitation, such as cultivated or commercially available ones.

The extraction process may be repeated several times, and thereafter, a step such as concentration or freeze-drying may be additionally performed. Specifically, the obtained extract is concentrated under reduced pressure to obtain a concentrate, and after freeze-drying the concentrate, a high concentration extract powder can be prepared by using a grinder. The extract also includes a fraction obtained by further fractionating the extract.

For supercritical extraction, the fluid may be one selected from the group consisting of carbon dioxide, nitrogen, nitrous oxide, methane, ethylene, propane and propylene, and may be carbon dioxide. Carbon dioxide has properties close to organic solvents in a supercritical state, and as the temperature and pressure increase, not only the general non-polar properties but also the polar properties are improved to exhibit amphoteric properties, thereby improving extractability. In addition, since carbon dioxide is vaporized under atmospheric pressure after extraction and released into the atmosphere, there is no residual solvent, so it is non-toxic, and has low risk, so it can be used to make an extract without contamination when manufacturing raw materials in the food or cosmetic field.

Supercritical extraction may be performed using an organic solvent together with supercritical carbon dioxide in order to increase the extraction yield of the active ingredient. That is, the extraction yield can be increased by using an organic solvent as an auxiliary solvent while performing extraction with a supercritical fluid. The organic solvent is the same as described in "1. Preparation of the fraction of Gaetbangpung extract" , and thus description is omitted.

The amount of Gaetbangpung input during the preparation of the supercritical extract may be 500g to 900g, 540g to 860g, 580g to 820g, 620g to 740g, or 660g to 700g. When the input amount of Gaetbangpung is within the above range, the obtained supercritical extract inhibits melanin synthesis to exhibit a whitening effect. If the input amount of Gaetbangpung is less than the lower limit, the whitening effect may decrease. When the input amount of Gaetbangpung exceeds the upper limit, the whitening effect is similar to the case of inputting raw materials within the above range, so raw materials are unnecessarily consumed in large amounts, and raw materials that have not been extracted under the extraction conditions are generated and discarded, resulting in production costs can be increased.

The temperature during the preparation of the supercritical extract may be 40 °C to 90 °C, 44 °C to 85 °C, 48 °C to 80 °C, 52 °C to 75 °C, 58 °C to 70 °C, or 58 °C to 65 °C. When the temperature range, the obtained supercritical extract exhibits a whitening effect by inhibiting melanin synthesis. When supercritical extraction is performed under a temperature condition outside the above range, the active ingredient with whitening effect is not extracted, or the yield is lowered even if it is extracted. This can be lowered.

When preparing the supercritical extract, the pressure may be 100 bar to 500 bar, 150 bar to 470 bar, 200 bar to 440 bar, 250 bar to 410 bar, or 300 bar to 380 bar. When the pressure range, the obtained supercritical extract inhibits melanin synthesis to exhibit a whitening effect. When supercritical extraction is performed under a pressure condition outside the above range, the active ingredient having a whitening effect is not extracted, or the yield is lowered even if it is extracted, and components not involved in the whitening effect are also extracted, so the content ratio of the whitening substance in the extract This can be lowered.

The preparation time of the supercritical extract may be 40 minutes to 140 minutes, 50 minutes to 130 minutes, 60 minutes to 120 minutes, 70 minutes to 110 minutes, or 80 minutes to 100 minutes. When the time range, the obtained supercritical extract exhibits a whitening effect by inhibiting melanin synthesis. When supercritical extraction is performed under a time condition outside of the above range, the active ingredient having a whitening effect is not extracted, or the yield is lowered even if it is extracted, and components not involved in the whitening effect are also extracted, so the content ratio of the whitening substance in the extract This can be lowered.

In a specific example of the present invention, 686.6 g of Gaetbangpung was put into a supercritical extractor, and the temperature of the extraction tank was maintained at 60° C. under a pressure of 350 bar to extract for 90 minutes to obtain a Gaetbangpung supercritical extract. When the obtained Gaetbangpung supercritical extract was treated on B16/F1 melanoma cells, melanin synthesis was inhibited at a level similar to that of arbutin, a positive control (see FIG. 4 ).

The gaetbangpung supercritical extract may be additionally subjected to a conventional purification process in order to enhance the effect of improving whitening, and the purified product obtained through the additional purification process is also included in the present invention. The purification process is the same as described in "1. Preparation of fractions of Gaetbangpung extract ", so description is omitted.

3. Cosmetic composition for skin whitening comprising hexane fraction of Gaetbangpung extract or Gaetbangpung supercritical extract

The method for preparing the hexane fraction of the Gaetbangpung extract and the method for preparing the Gaetbangpung supercritical extract are the same as those described in "1. Preparation of the fraction of Gaetbangpung extract ", and "2. Preparation of the Gaetbangpung supercritical extract". omit the description.

The cosmetic composition for whitening of the present invention provides an excellent whitening effect by fundamentally inhibiting melanin production, and can improve skin pigmentation and skin tone down. The skin whitening inhibits the synthesis of melanin to show all actions to inhibit or prevent the deposition of melanin pigment on the skin, and the skin deposition of the pigment refers to any action or state in which the color of the skin is darkened due to the increase in the synthesis of melanin. .

The cosmetic composition of the present invention may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form, for example, but not limited thereto, may include, but are not limited to, the form of a lotion, cream, lotion, bathing agent, and the like.

Bases, adjuvants and additives commonly used in the cosmetic field are not particularly limited, and examples thereof include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin, and the like.

When the composition of the present invention is prepared as a cosmetic composition, the composition of the present invention may include not only the hexane fraction of the Gaetbangpung extract or the Gaetbangpung supercritical extract, but also ingredients commonly used in cosmetic compositions, such as antioxidants, It may contain conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkyl amidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used.

The cosmetic composition of the present invention may be used alone or in overlapping application, or may be used in overlapping application with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having excellent skin moisturizing effect and skin barrier improvement effect according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the present invention will be described in detail by way of Preparation Examples and Examples.

However, the following Preparation Examples and Examples are only for illustrating the present invention, and the content of the present invention is not limited by the following Preparation Examples and Examples.

Preparation Example 1. Preparation of Gaetbangpung Ethanol Extract

Gaetbangpung ( Glehnia littoralis ) 70% (v/v) aqueous ethanol solution was added to 10 g of powder and extracted at 25° C. for 24 hours to obtain an ethanol extract of Gaetbangpung. The obtained Gaetbangpung ethanol was concentrated under reduced pressure and dried to obtain 3.51 g of Gaetbangpung ethanol extract powder.

Preparation Example 2. Preparation of hexane fraction of Gaetbangpung ethanol extract

Compared to 3.51 g of the ethanol extract powder of Gaetbangpung obtained in Preparation Example 1, 200 ml of an organic solvent was added to obtain a fraction. Hexane, methylene chloride, ethyl acetate, and butanol were used as organic solvents, and were used for fractionation in the above order. After treatment with an organic solvent, it was allowed to stand at room temperature for 24 hours. When the organic solvent layer and the water layer were separated, only the organic solvent layer was recovered and used as a sample, and another organic solvent was added to the residual water layer to perform secondary to quaternary fractionation. Fig. 1 shows the fractionation method. The obtained organic solvent layer was concentrated under reduced pressure and dried to obtain 0.70 g of a hexane fraction, 0.41 g of a methylene chloride fraction, 0.33 g of an ethyl acetate fraction, and 0.29 g of a butanol fraction.

Preparation Example 3. Preparation of Gaetbangpung Supercritical Extract

After putting 686.6 g of Gaetbangpung into the supercritical extractor, carbon dioxide in the supercritical state was supplied to the extractor through a _{CO 2 circulation pump.} The supercritical condition was maintained by controlling the temperature with a preheater and controlling the pressure using a pressure controller. A pressure of 350 bar was applied, and the extraction tank temperature was maintained at 60° C. for 90 minutes to obtain a Gaetbangpung supercritical extract. The cumulative extraction amount according to the extraction time is shown in Table 1 and FIG. 2 below.

extraction time
(minute) 0 10 20 30 40 50 60 70 80 90 Cumulative extract
(g) 0 2.9 7.6 10.0 10.8 11.4 11.8 12.2 12.7 13.0

As a result of the measurement, the extraction amount of the Gaetbangpung supercritical extract was 13.0 g, and the extraction yield was 1.9%.

Experimental Example 1. Evaluation of whitening efficacy of fractions of Gaetbangpung extract

B16/F1 melanoma cells (ATCC, USA) were grown up to passage 10, and then 5×10 ⁴ were aliquoted into 6-well plates. The cells were treated with DMEM medium containing 10% bovine serum, and cultured at _{37° C., 5% CO 2 in an incubator.} After 24 hours, the hexane fraction, methylene chloride fraction, ethyl acetate fraction, and butanol fraction of the Gaetbangpung ethanol extract prepared in Preparation Example 2 were treated at concentrations of 3.125 µg/ml, 6.25 µg/ml, and 12.5 µg/ml, respectively, and positive As a control, p-coumaric acid was treated at a concentration of 50 μg/ml. The water layer remaining after fractionation with hexane or the water layer remaining after fractionation with butanol was treated at concentrations of 25 μg/ml, 50 μg/ml, and 100 μg/ml, respectively. After 72 hours, the melanoma cells were physically removed and the melanin was dissolved at 70° C. using 1N NaOH containing DMSO, and then the absorbance was measured at 405 nm.

As shown in FIG. 3 , the butanol fraction of the Gaetbangpung ethanol extract exhibited melanin synthesis inhibitory efficacy at a concentration of 12.5 μg/ml, similar to that of the positive control group. The treatment concentration of the positive control group was 50 μg/ml, and the treatment concentration of the butanol fraction was 12.5 μg/ml, so it was found that even when treated at a concentration of about 1/4 compared to the positive control group, it was found that it similarly exhibited the effect of inhibiting melanin synthesis. . In addition, the water layer, a residue remaining after the hexane fractionation, did not inhibit melanin synthesis even when treated at a high concentration of 100 μg/ml, and the fractions fractionated with other organic solvents did not inhibit melanin synthesis.

From the above results, even the ethanol extract of Gaetbangpung had a different effect of blocking melanin synthesis depending on the solvent used for fractionation.

Experimental Example 2. Evaluation of whitening efficacy of Gaetbangpung supercritical extract

The whitening efficacy was evaluated in the same manner as in Experimental Example 1, except that the sample for evaluating the whitening efficacy was changed to the Gaetbangpung supercritical extract of Preparation Example 3, and arbutin was used as a positive control. Gaetbangpung supercritical extract of Preparation Example 3 was treated at a concentration of 12.5 μg/ml, 25 μg/ml, and 50 μg/ml, and arbutin was treated at a concentration of 250 μg/ml. In order to compare the whitening effect of the Gaetbangpung supercritical extract, the hexane fraction of the Gaetbangpung ethanol extract, which had the best effect among the Gaetbangpung fractions prepared in Preparation Example 2, was used at 3.125 µg/ml, 6.25 µg/ml, and 12.5 µg/ml. concentration was treated.

As a result of the evaluation, as shown in FIG. 4 , the supercritical extract of Gaetbangpung inhibited melanin synthesis to a degree similar to arbutin used as a positive control, and thus had excellent whitening efficacy. Considering that Gaetbangpung supercritical extract was treated at 50 μg/ml and arbutin was treated at 250 μg/ml, it can be seen that Gaetbangpung supercritical extract inhibits melanin synthesis similarly to arbutin even at a low concentration. . In addition, the hexane fraction of Gaetbangpung ethanol extract, which was excellent in effect in Preparation Example 2, inhibited melanin synthesis to a similar degree when treated with 12.5 μg/ml and treated with 25 μg/ml of Gaetbangpung supercritical extract. The hexane fraction of the Gaetbangpung ethanol extract showed cytotoxicity when treated in excess of 12.5 μg/ml, whereas the Gaetbangpung supercritical extract did not show cytotoxicity up to 50 μg/ml. It was confirmed that it is safer and has excellent melanin synthesis.

Through the above results, it can be seen that the Gaetbangpung supercritical extract exhibits a whitening effect similar to that of arbutin, so that the Gaetbangpung supercritical extract can be used for the purpose of whitening instead of arbutin.

Preparation Example 4. Preparation of cosmetic formulations

4-1. Preparation of Essence

Essence was prepared according to the content (parts by weight) shown in Table 2 below using the hexane fraction of the Gaetbangpung extract or the Gaetbangpung supercritical extract.

Furtherance Content (parts by weight) triethanolamine 0.25 Carboxyvinyl Polymer 0.22 glycerin 4 butylene glycol 2 Hexane fraction of Gaetbangpung extract or Gaetbangpung supercritical extract 1.5 beeswax 0.5 cetostearyl alcohol One Glyceryl Monostearate One squalene 4 Purified water appropriate amount

4-2. Manufacture of softened lotion

Softening lotion containing the hexane fraction of Gaetbangpung extract or Gaetbangpung supercritical extract as an active ingredient was prepared as shown in Table 3 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium EDIT 0.05 allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric Acid 0.01 sodium citrate 0.02 Glyceres-26 1.00 arbutin 2.00 PEG-40
hydrogenated castor oil 1.00 ethanol 30.00 Hexane fraction of Gaetbangpung extract or Gaetbangpung supercritical extract 0.5 coloring agent a very small amount flavoring agent a very small amount Purified water remaining amount

4-3. Preparation of nourishing cream

A nutrient cream containing the hexane fraction of Gaetbangpung extract or Gaetbangpung supercritical extract as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic Acid 2.0 Polysorbate 60 1.5 Lipophilic Glyceryl Stearate 2.0 Sorbitan sesquioleate 1.5 cetearyl alcohol 3.0 mineral oil 4.0 squalene 3.8 Hexane fraction of Gaetbangpung extract or Gaetbangpung supercritical extract 1.5 vegetable oil 1.8 dimethicone 0.4 Dipotassium glycyrrhizate a very small amount allantoin a very small amount Sodium Hyaluronate a very small amount Tocopheryl Acetate appropriate amount triethanolamine appropriate amount flavoring agent appropriate amount Purified water remaining amount

4-4. manufacture of lotions

A lotion containing the hexane fraction of the Gaetbangpung extract or the Gaetbangpung supercritical extract as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) cetostearyl alcohol 1.6 stearic acid 1.4 lipophilic monostearate glycerin 1.8 PEG-100 Stearate 2.6 Sorbital Sesquioleate 0.6 squalene 4.8 macadaia oil 2 jojoba oil 2 tocopherol acetate 0.4 Methylpolysiloxane 0.2 tocopherol acetate 0.4 1,3-butylene glycol 4 Xanthan Gum 0.1 glycerin 4 d-pandenol 0.15 Hexane fraction of Gaetbangpung extract or Gaetbangpung supercritical extract 1.0 allantoin 0.1 Carbomer (2% aq. Sol) 4 triethanolamine 0.15 ethanol 3 Purified water appropriate amount

Claims (10)

A cosmetic composition for skin whitening comprising a Gaetbangpung supercritical extract, wherein the supercritical fluid used in preparing the supercritical extract is carbon dioxide. delete delete delete delete The method according to claim 1,
The skin whitening cosmetic composition for skin whitening that suppresses pigmentation caused by melanin synthesis. delete The method according to claim 1,
A cosmetic composition for skin whitening wherein the temperature during the preparation of the supercritical extract is 40°C to 90°C. The method according to claim 1,
A cosmetic composition for skin whitening wherein the pressure during preparation of the supercritical extract is 100 bar to 500 bar. The method according to claim 1,
The preparation time of the supercritical extract is 40 minutes to 140 minutes for skin whitening cosmetic composition.

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