CN110934148A - Compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam and application thereof - Google Patents
Compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam and application thereof Download PDFInfo
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Abstract
The invention discloses a compound pesticide of Metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam and application thereof. The invention firstly provides a compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam, wherein the compound pesticide contains the metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam. The metarhizium anisopliae mutant strain MaUV-1 has the characteristics of ultraviolet resistance and high temperature resistance, and has good compatibility with thiamethoxam, the thiamethoxam does not influence spore germination and hypha growth of the metarhizium anisopliae mutant strain MaUV-1, and a compound insecticide obtained by compounding the metarhizium anisopliae mutant strain MaUV-1 and the thiamethoxam has a synergistic effect, so that the control effect on rice planthoppers and rice leafhoppers can be remarkably improved, and the using amount of a chemical pesticide thiamethoxam is reduced; in addition, the compound pesticide has stable prevention and control effect on rice pests, is not influenced by field environmental factors such as high temperature, strong ultraviolet rays and the like, and has good application prospect in prevention and control of rice planthoppers and rice leafhoppers.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a compound pesticide of metarhizium anisopliae (Metarhizium anisopliae) mutant strain MaUV-1 and thiamethoxam and application thereof.
Background
In southern rice planting areas in China, rice pests such as rice planthoppers and rice leafhoppers absorb rice juice through thorns and can also spread virus diseases such as rice dwarf and the like, so that large-area yield reduction and even no harvest of rice are caused. Thiamethoxam is a neonicotinoid insecticide and has the characteristics of contact poisoning, stomach toxicity, systemic property, long lasting period and the like; the thiamethoxam sprayed on the leaf surface can be absorbed by the plant quickly and conducted to each part of the plant, has good control effect on sucking mouthparts pests such as aphids, leafhoppers, plant hoppers, thrips, whiteflies and the like, and has low toxicity to mammals. However, in recent years, due to the wide and large-scale use of pesticides such as thiamethoxam on rice, rice pests such as rice planthoppers and rice leafhoppers have high-level drug resistance, chemical pesticides in agricultural products have serious residues, and meanwhile, natural enemies of the pests are killed, and the ecological system of the rice field is damaged.
Metarrhizium anisopliae is one of important entomopathogenic fungi, has a completely new action mechanism different from that of the existing chemical insecticide, has the advantages of no pollution to the environment and no residue, and is commonly used for preventing and controlling rice leafhoppers, brown planthoppers, diamond back moths and other pests. However, field application practices show that the control effect of live microbial insecticides such as metarhizium anisopliae is unstable and is easily influenced by field environmental conditions such as strong ultraviolet rays and high temperature in southern areas. Therefore, the pesticide resistance problem of rice planthoppers, rice leafhoppers and other rice pests is solved, the rice pests are efficiently prevented and controlled, and the important significance is achieved in improving the yield and the quality of rice.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the prior art and provide a compound pesticide of Metarhizium anisopliae (Metarhizium anisopliae) mutant strain MaUV-1 and thiamethoxam and application thereof.
The invention aims to provide a compound insecticide.
The invention also aims to provide application of the compound pesticide in controlling rice planthoppers/rice leafhoppers or preparing a biological agent for controlling the rice planthoppers/the rice leafhoppers.
Still another object of the present invention is to provide a biological agent for controlling rice planthopper/rice leafhopper.
The above purpose of the invention is realized by the following technical scheme:
the invention firstly provides a compound insecticide which contains metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam.
Preferably, the mass ratio of the metarhizium anisopliae mutant strain MaUV-1 to the thiamethoxam is 100: 1.67-6.67.
More preferably, the mass ratio of the metarhizium anisopliae mutant strain MaUV-1 to the thiamethoxam is 100: 3-4.
Still more preferably, the metarhizium anisopliae mutant strain MaUV-1 and the thiamethoxam are in a mass ratio of 100: 3.
The metarhizium anisopliae mutant strain MaUV-1 is preserved in the China Center for Type Culture Collection (CCTCC) in 2016, 5 months and 12 days, and the preservation number is CCTCC NO: m2016250, the preservation address is Wuhan, Wuhan university in China.
Preferably, the metarhizium anisopliae mutant strain MaUV-1 is spore powder of the metarhizium anisopliae mutant strain MaUV-1.
The metarhizium anisopliae mutant strain MaUV-1 and the thiamethoxam have good biocompatibility, and the low-concentration thiamethoxam and metarhizium anisopliae mutant strain MaUV-1 are compounded to obtain the compound insecticide which has obvious control effect on rice planthoppers and rice leafhoppers, can improve the quick-acting performance of the fungal insecticide, reduce the using amount of chemical pesticides and reduce the residue of the chemical pesticides on agricultural products; meanwhile, the anti-ultraviolet and high-temperature-resistant metarhizium anisopliae mutant strain MaUV-1 has stable control effect on rice pests such as rice planthoppers, rice leafhoppers and the like, is not influenced by field environmental conditions such as high temperature, strong ultraviolet rays and the like in southern areas, has no pollution to ecological environment, and can continuously control the rice pests; therefore, the following should be within the scope of the present invention:
the compound pesticide is applied to controlling rice planthoppers/rice leafhoppers or preparing a biological preparation for controlling the rice planthoppers/the rice leafhoppers.
In addition, the invention also provides a biological agent for preventing and controlling rice planthoppers/rice leafhoppers, which consists of a main agent and an auxiliary agent; the main agent comprises metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam, and the auxiliary agent comprises a stabilizer, an auxiliary agent and a filler.
Preferably, the biological agent comprises, in mass percent: 32 to 61 percent of main agent, 0.2 to 0.8 percent of stabilizing agent, 11 to 15 percent of auxiliary agent and 23.6 to 56.8 percent of filler.
Preferably, the stabilizer is any one or more of ascorbic acid or nano aluminum hydroxide.
Preferably, the auxiliary agent is any one or more of MF-5, sodium lignosulfonate, a wetting-retarding agent DY6, Tween-80, MorwetEF-W, sodium carboxymethylcellulose, span-20, nekal LX or OP-10.
Preferably, the filler is any one or more of diatomite, attapulgite or paraffin oil.
Preferably, the biological preparation is in the form of wettable powder or oil.
The invention has the following beneficial effects:
the invention provides a compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam and application thereof. The compound pesticide takes the metarhizium anisopliae mutant strains MaUV-1 and thiamethoxam which are ultraviolet-resistant and high-temperature-resistant as effective components, the metarhizium anisopliae mutant strains MaUV-1 and thiamethoxam have good compatibility, and the low-concentration thiamethoxam and metarhizium anisopliae mutant strains MaUV-1 have synergistic effect by compounding, so that the insecticidal effect on rice plant hoppers, rice leafhoppers and other rice pests is obviously improved, the using amount of the chemical pesticide thiamethoxam is reduced, the drug resistance of the rice pests on the chemical pesticide thiamethoxam can be improved, the drug resistance increase speed of the rice plant hoppers, the rice leafhoppers and other rice pests on the existing chemical pesticide is slowed down, and the residue of the chemical pesticide is reduced; the compound pesticide has stable control effect on rice pests, is not influenced by field environmental factors such as high temperature, strong ultraviolet rays and the like in southern areas, is beneficial to protecting natural enemies of the pests, continuously controlling the damage of the rice pests, promoting the improvement of the yield and the quality of the rice, has no pollution to the environment, and has good application prospect.
Drawings
FIG. 1 is a diagram showing symptoms of rice leafhoppers after being infected by metarhizium anisopliae mutant strain MaUV-1.
FIG. 2 is a graph showing the results of the ultraviolet resistance test of metarhizium anisopliae mutant strain MaUV-1.
FIG. 3 is a high temperature resistance test result diagram of metarhizium anisopliae mutant strain MaUV-1.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 acquisition of Metarhizium anisopliae mutant Strain MaUV-1 and infection of rice leafhoppers
The inventor group collects larvae of naturally infected scarab (Phyllopertha horticola L.) in Tibet area, and obtains the wild strain of the metarhizium anisopliae after separation and screening from the larvae. The control effect of the strain on rice pests is found to be unstable in later-stage field application, and the strain is easily influenced by field environmental conditions such as strong ultraviolet rays, high temperature and the like in southern areas; therefore, the wild strain is irradiated by ultraviolet rays to induce the wild strain to generate variation, and the mutant strain MaUV-1 of the metarhizium anisopliae with ultraviolet resistance and high temperature resistance is finally obtained through screening and purification and is preserved in the China Center for Type Culture Collection (CCTCC) in 2016, 5, 12 and the preservation number is CCTCC NO: m2016250, the preservation address is Wuhan, Wuhan university in China.
The symptoms of rice leafhoppers infected by metarhizium anisopliae mutant strain MaUV-1 are shown in figure 1. The metarhizium anisopliae mutant strain MaUV-1 can effectively infect the rice leafhoppers and cause mass death of the rice leafhoppers. After the rice leafhoppers are infected by the metarhizium anisopliae, covered white hyphae can be seen at the head, the ventral foot and other parts of the rice leafhoppers on 3-5 days (A picture in figure 1), and covered dark green spore powder can be seen at the head, the ventral foot and other parts of the rice leafhoppers on 8-10 days (B picture in figure 1).
Example 2 Metarhizium anisopliae mutant strain MaUV-1 compatibility determination for thiamethoxam
1. Experimental methods
Inoculating metarhizium anisopliae mutant strain MaUV-1To Chachi's medium (sucrose 30.0g, sodium nitrate (NaNO)3)3.0g of dipotassium hydrogen phosphate (K)2HPO4)1.0g magnesium sulfate (MgSO)4.7H20.5g of O), 0.5g of potassium chloride (KCl), and ferrous sulfate (FeSO)4)0.01g and 20g of agar powder are added into water to make the total volume be 1000mL), the mixture is cultured in a thermostat (L: D is 14:10) at 26 +/-1 ℃ until sporulation occurs, then 0.1% Tween-80 sterile water is used for collecting spores, fungal spores and thiamethoxam are mixed in a Chachi culture solution to prepare suspensions containing thiamethoxam (0.1x, 0.5x and 1x) and fungal spores with different concentrations, and the suspensions are tested by a glass slide germination method;
dropping the thiamethoxam and spore suspension with different concentrations on a sterile glass slide, placing the sterile glass slide on a culture dish with filter paper laid at the bottom, adding sterile water for moisturizing (100, RH), culturing for 21h, 24h and 27h, performing microscopic examination, and calculating the spore germination rate (the spore germination rate is the number of germinated spores/total spore number multiplied by 100%). Each treatment was repeated 3 times. All the test data are processed and completed on a data processing software SAS system.
2. Results of the experiment
The results of the spore germination rates of metarhizium anisopliae mutant strain MaUV-1 in thiamethoxam solutions with different concentrations are shown in Table 1, and it can be seen from the table that compared with a control group, the spore germination rates in the thiamethoxam solutions with 21h, 1x and 0.5x times of conventional concentrations are both obviously reduced, and the thiamethoxam with 0.1x times of conventional concentrations does not influence the spore germination of metarhizium anisopliae; after 27h, the difference of the spore germination rates of the thiamethoxam solutions with different concentrations is not significant compared with that of a control group, and the spore germination rate in each treatment area exceeds 84.0%; the 1x and 0.5x times conventional concentrations of thiamethoxam are only used for temporarily inhibiting the spore germination of metarhizium anisopliae mutant strain MaUV-1, the inhibition gradually disappears along with the prolonging of time, and the thiamethoxam has no inhibition on the spore germination of fungi after 27 h. The results show that the metarhizium anisopliae mutant strain MaUV-1 has better compatibility with thiamethoxam.
TABLE 1 spore germination rate results of metarhizium anisopliae mutant strain MaUV-1 in thiamethoxam solutions of different concentrations
| Time of day | Control group | Thiamethoxam (1x) | Thiamethoxam (0.5x) | Thiamethoxam (0.1x) |
| 21h | 58.7±2.7a | 41.7±2.3d | 48.1±2.4c | 54.8±3.8a |
| 24h | 82.6±4.5a | 65.9±3.3b | 79.8±4.2a | 81.7±4.2a |
| 27h | 89.2±3.1a | 84.1±3.6a | 86.7±4.5a | 88.9±3.1a |
Note: the same letters after the same number in the same column in the table indicate that the difference is not significant (DMRT method); thiamethoxam (1x), thiamethoxam (0.5x) and thiamethoxam (0.1x) respectively represent the dose of thiamethoxam used as a field conventional dose, and 0.5 and 0.1 times of the field conventional dose.
Example 3 high temperature and ultraviolet resistance test of Metarhizium anisopliae mutant Strain MaUV-1
1. Experimental methods
Inoculating conidia of wild metarhizium anisopliae (WT) and mutant (MaUV-1) to PDA plate, culturing, collecting spores of all strains with 0.1% Tween-80 sterile water, and making into 1.0 × 106spore/mL spore suspension is placed under ultraviolet irradiation for 0 and 40min, then 5mL spore suspension is added into a triangular flask filled with 200mL Chashi culture solution, the culture is carried out at 26 +/-1 ℃ and 200rpm, spore germination is observed and recorded at 24h and 27h, and the germination standard is that the length of a bud tube exceeds half of the diameter of the spore. All treatments were repeated 3 times. The ultraviolet resistance of the metarhizium anisopliae mutant strain MaUV-1 is determined by comparing the difference of the strains at the 24 th and 27 th hours in the spore germination rate.
In addition, spores of all strains were collected with 0.1% Tween-80 sterile water and formulated into 1.0X 106spore/mL, then 5mL of spore suspension is added into a triangular flask containing 200mL of Chashi culture solution, the mixture is cultured under the conditions of 28 +/-1 ℃, 34 +/-1 ℃, 35 +/-1 ℃, 37 +/-1 ℃, 38 +/-1 ℃ and 200rpm, the germination condition of the spores is observed and recorded at 24h and 27h, and the germination standard is that the length of a bud tube exceeds half of the diameter of the spores. All treatments were repeated 3 times. The high temperature resistance of the metarhizium anisopliae mutant strain MaUV-1 is determined by comparing the difference of spore germination rates of the strains at different temperatures in the 24 th and 27 th hours.
2. Results of the experiment
The ultraviolet resistance test result of the metarhizium anisopliae mutant strain MaUV-1 is shown in figure 2, and it can be seen from the figure that after the metarhizium anisopliae mutant strain MaUV-1 is irradiated by ultraviolet rays for 40min, the difference of the spore germination rates of 21h, 24h and 27h is not significant compared with that of a control group (irradiated by ultraviolet rays for 0min), while after the wild strain is irradiated by ultraviolet rays for 40min, the difference of the spore germination rate of the wild strain is significant compared with that of the control group, and the spore germination rate of a treatment area is significantly reduced.
The result of the high temperature resistance test of the metarhizium anisopliae mutant strain MaUV-1 is shown in figure 3, and it can be seen from the figure that the spore germination rate of the metarhizium anisopliae mutant strain MaUV-1 at the high temperature of 37 ℃ is more than 30%, while the spore does not germinate in the wild strain at the temperature of 35 ℃.
The above results illustrate that: the metarhizium anisopliae mutant strain MaUV-1 has good ultraviolet resistance and high temperature resistance.
Example 450% wettable powder of Metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam
A wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam comprises the following specific formula components: 50% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 2% thiamethoxam (commercial raw pesticide), 0.3% ascorbic acid, 3% MF-5, 6% sodium lignosulfonate, 5% suspending agent DY6, and diatomaceous earth supplementing to 100% to prepare 50% wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to thiamethoxam is 100: 4.
Example 530% wettable powder of Metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam
A wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam comprises the following specific formula components: 30% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 2% thiamethoxam (commercial raw pesticide), 0.2% ascorbic acid, 2% MF-5, 4% sodium lignosulfonate, 5% suspending agent DY6, and diatomaceous earth supplementing to 100% to prepare 30% wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to thiamethoxam is 100: 6.67.
Example 660% wettable powder of Metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam
A wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam comprises the following specific formula components: 60% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spores/g), 1% thiamethoxam (commercial technical), 0.4% ascorbic acid, 4% MF-5, 6%Sodium lignosulphonate, 5 percent of Darun suspending agent DY6 and diatomite which are supplemented to 100 percent to prepare 60 percent wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to thiamethoxam is 100: 1.67.
Example 750% wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam
A wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam comprises the following specific formula components: 50% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 1.5% thiamethoxam (commercial raw pesticide), 0.8% nano-aluminum hydroxide, 3% tween-80, 5% Morwet EF-W, 5% sodium carboxymethylcellulose and attapulgite to 100%, so as to prepare 50% wettable powder of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to thiamethoxam is 100: 3.
Example oil solution of 850% Metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam
An oil solution of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam, which comprises the following specific formula components: 50% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 1.5% thiamethoxam (commercial raw pesticide), 0.8% nano-aluminum hydroxide, 5% span-20, 3% nekal LX, 5% OP-10, adding paraffin oil to supplement to 100% to prepare 50% metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam oil; wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to thiamethoxam is 100: 3.
Comparative example 1 Metarhizium anisopliae mutant strains MaUV-1 and thiamethoxam in compounded agent
A wettable powder, except there are no metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam, the other formulation component content is the same as example 4; 0.3 percent of ascorbic acid, 3 percent of MF-5, 6 percent of sodium lignosulphonate, 5 percent of wetting suspending agent DY6 and 100 percent of diatomite to prepare wettable powder (the using concentration of metarhizium anisopliae mutant strains MaUV-1 and thiamethoxam is 0).
Comparative example 2 absence of thiamethoxam in the combination
Except that the thiamethoxam is not contained, the contents of the other formula components are the same as those in the embodiment 4; the specific formula comprises the following components in percentage by weight: 60% metarhizium anisopliae mutant strain MaUV-1 spore powder (10)11Spore/g), 0.3% ascorbic acid, 3% MF-5, 6% sodium lignosulfonate, 5% suspending agent DY6, and diatomaceous earth supplementing to 100% to obtain wettable powder (the use concentration of metarhizium anisopliae mutant strain MaUV-1 is 3x 10)7spores/mL).
Comparative example 3 lack of Metarhizium anisopliae mutant strain MaUV-1 in compounded agent
A wettable powder, except there is no Metarhizium anisopliae mutant strain MaUV-1, the other formulation component content is the same as example 4; the specific formula comprises the following components in percentage by weight: 2% thiamethoxam (commercial raw pesticide), 0.3% ascorbic acid, 3% MF-5, 6% sodium lignosulfonate, 5% suspending agent DK8 and diatomite which are supplemented to 100% to prepare wettable powder (the using concentration of the thiamethoxam is 10 mug/mL).
Comparative example 4
25% thiamethoxam, at a conventional field dosage (3.0 g/mu/30 kg water, 25. mu.g/mL).
Application example 1 determination of toxicity of compound insecticide on electric leafhopper
Carrying out a toxicity determination experiment by using the compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam prepared in the embodiments 4-8 of the invention and the wettable powder prepared in the comparative examples 1-4, and determining the toxicity of the compound pesticide on electric light leafhoppers; the specific experimental methods and experimental results are as follows:
1. experimental methods
The test plant is rice (Oryza sativa L.), potted seedlings (the diameter of a pot is 30cm, the height of the seedlings is 40cm, 3 clusters/pot and 6-13 plants/cluster) are placed in an insect cage, adult electrooptic leafhoppers are inoculated, all the adult electrooptic leafhoppers are driven out after spawning, the electrooptic leafhoppers are developed to 4-year-old nymphs for later use, and all the tests are carried out in a net room (32-37 ℃);
diluting the compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam prepared in the embodiments 4-8 and the wettable powder prepared in the comparative examples 1-3 by 2000 times to respectively obtain mixed solutions with different concentrations; the thiamethoxam in the comparative example 4 is prepared into liquid medicine with the concentration (20 microgram/mL) which is conventionally used in the field, then the liquid medicine is respectively sprayed on the leaves and stems of the rice of different treatment groups by adopting a spraying method, the rice is well stained with liquid medicine fog drops instead of dripping, each seedling is treated for 6 pots, the effect of 5 days and 10 days after the pesticide is investigated, the corrected death rate of the electric leafhopper is calculated, and the whole experiment is repeated for 4 times. The corrected mortality rate was calculated according to the following formula:
corrected mortality (%) — treatment mortality-control mortality)/(1-control mortality) × 100.
2. Results of the experiment
The toxicity test results of the compound insecticide prepared by the invention on the electric leafhoppers are shown in table 2, and it can be seen from the table that when the metarhizium anisopliae mutant strains MaUV-1 and thiamethoxam are not contained in the wettable powder (comparative example 1), the control effect of the electric leafhoppers is only 8.6% (5 days) and 11.2% (10 days); when the wettable powder only contains thiamethoxam, the control effect of the electric cicada is respectively 50.4% (comparison example 3, 10 days of a 10PPM treatment area of thiamethoxam) and 73.6% (comparison example 4, 10 days of a 25PPM treatment area of thiamethoxam); when the wettable powder only contains metarhizium anisopliae mutant strain MaUV-1 (comparative example 2), the control effect of the electric leafhopper is 34.3 percent (5 days) and 79.1 percent (10 days); the compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam (example 4-8) has the control effect on the electric leafhopper of 80.0-86.7% (5 days) and 88.8-95.4% (10 days). The compound pesticide of metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam prepared in the examples 4-8 has a control effect on electric leafhoppers which is remarkably higher than that of wettable powder prepared in the comparative examples 1-3, and is remarkably higher than that of the conventional field recommended use concentration of thiamethoxam in the comparative example 4.
In addition, the control effect of the compound pesticide on the electric leafhoppers is obviously higher than that of a single pesticide, the control effect is improved and the insecticidal time is shortened compared with that of a fungal pesticide, and the control effect is improved and the control duration is prolonged compared with that of a chemical pesticide. Wherein, the single strain of Metarhizium anisopliaeAgent (Metarhizium anisopliae MaUV-1 application concentration of 3x107spore/mL), the control effect of the compound pesticide on the electric leafhopper is improved by 133.2-152.7 percent (day 5) and 12.3-20.6 percent (day 10), which shows that the quick acting performance of the pesticide is improved after the thiamethoxam is added into the Isaria javanicus pesticide. Compared with the single chemical pesticide thiamethoxam preparation (the using concentration of thiamethoxam is 10 mug/mL), the control effect of the compound pesticide on the electric leafhopper is improved by 92.7-108.9 percent (day 5) and 76.2-89.3 percent (day 10), which shows that after the chemical pesticide is added with the fungal pesticide, the control effect is improved, and the effective control time of the pesticide is prolonged. Compared with the chemical pesticide with the conventional dosage, the compound pesticide improves the prevention and treatment effect and reduces the dosage of the chemical pesticide by 150 percent.
The results show that the compound pesticide of the metarhizium anisopliae mutant strain MaUV-1 and the thiamethoxam prepared by the invention has an obvious prevention and treatment effect on electric leafhoppers, the compound pesticide of the metarhizium anisopliae mutant strain MaUV-1 and the thiamethoxam can play a role in synergy, the prevention and treatment effect of the compound pesticide on the electric leafhoppers is obviously higher than that of a single agent, the quick-acting property of the pesticide is improved while the prevention and treatment effect is improved, the effective prevention and treatment time is prolonged, and the using amount of chemical pesticides is reduced.
TABLE 2 toxicity test results of the built insecticide prepared by the invention on electric leafhopper (2000 times diluted built insecticide)
Note: the same letters after the same numbers in the same column indicate no significant difference (DMRT method).
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. The compound insecticide is characterized by comprising metarhizium anisopliae mutant strains MaUV-1 and thiamethoxam.
2. The compound insecticide as claimed in claim 1, wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to thiamethoxam is 100: 1.67-6.67.
3. The compound insecticide as claimed in claim 2, wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to thiamethoxam is 100: 3-4.
4. The compound pesticide according to claim 3, wherein the mass ratio of metarhizium anisopliae mutant strain MaUV-1 to thiamethoxam is 100: 3.
5. The compound pesticide as claimed in any one of claims 1 to 4, wherein the metarhizium anisopliae mutant strain MaUV-1 has been preserved in the China Center for Type Culture Collection (CCTCC) at 2016, 5, 12 and has a preservation number of CCTCC NO: m2016250.
6. The compound pesticide according to claim 5, wherein the metarhizium anisopliae mutant strain MaUV-1 is spore powder of the metarhizium anisopliae mutant strain MaUV-1.
7. The application of the compound pesticide as claimed in any one of claims 1 to 6 in controlling rice planthoppers/rice leafhoppers or preparing biological agents for controlling the rice planthoppers/the rice leafhoppers.
8. The biological preparation for preventing and controlling rice planthoppers/rice leafhoppers is characterized by consisting of a main agent and an auxiliary agent; the main agent comprises metarhizium anisopliae mutant strain MaUV-1 and thiamethoxam, and the auxiliary agent comprises a stabilizer, an auxiliary agent and a filler.
9. The biological agent according to claim 8, characterized in that it comprises, in mass percent: 32 to 61 percent of main agent, 0.2 to 0.8 percent of stabilizing agent, 11 to 15 percent of auxiliary agent and 23.6 to 56.8 percent of filler.
10. The biological preparation according to claim 8 or 9, wherein the biological preparation is in the form of wettable powder or oil.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114342959A (en) * | 2022-01-18 | 2022-04-15 | 山东省农业科学院 | Composition for simultaneously preventing and treating cucumber aphid and cucumber bacterial angular leaf spot |
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