CN101502270B - Compositional main pesticide containing Paecilomyces javanicus and Imidacloprid and pesticide - Google Patents
Compositional main pesticide containing Paecilomyces javanicus and Imidacloprid and pesticide Download PDFInfo
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- CN101502270B CN101502270B CN2009100379996A CN200910037999A CN101502270B CN 101502270 B CN101502270 B CN 101502270B CN 2009100379996 A CN2009100379996 A CN 2009100379996A CN 200910037999 A CN200910037999 A CN 200910037999A CN 101502270 B CN101502270 B CN 101502270B
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- imidacloprid
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- insecticide
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- paecilomyces javanicus
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Abstract
The present invention discloses an insect disinfestation main agent compounded by paecilomyces javanicus and imidacloprid and an insecticide. The main agent is the mixture of paecilomyces javanicus and imidacloprid. The insecticide is composed of the main agent and the assistant agent. The main agent accounts for 12.5-55% and the assistant agent accounts for 45-87.5% according to weight percentage. The compounded insecticide according to the invention can be processed to water dispersible powder, suspending agent or oil solution. After long-time infection biology research and indoor biological assay, the insecticide has strong infection insect disinfestation effect to a plurality of insects, especially the piercing-sucking mouthparts insects. The insecticide of the invention has excellent control effect to the insects of diamondback moths, aphids, thrips, etc.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, be meant the Paecilomyces javanicus bacterium and the composite desinsection host and the insecticide of Imidacloprid of a kind of environmental protection, pollution-free, noresidue especially.
Background technology
Paecilomyces javanicus (Isaria javanicus (Friendrichs ﹠amp; Bally)) be a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, have features such as the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use Paecilomyces javanicus has important economic implications.Paecilomyces javanicus distributes extensively, red fire ant, prodenia litura, aphid, aleyrodid and diamond-back moth etc. is had good control efficiency, has broad application prospects.
Imidacloprid (imidacloprid) is widely used efficient, interior suction at present, broad spectrum type nicotinic insecticide, is used to prevent and treat perverse suction formula mouthpart insects such as prodenia litura, aleyrodid and diamond-back moth.Along with using historical prolongation, insect strengthens day by day to the pesticide resistance of Imidacloprid.Adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, and therefore, the mixed ratio of screening Paecilomyces javanicus and Imidacloprid the best has great importance.Still find no at present the relevant paecilomyces fumosoroseus and the compounded pesticides of beta-cypermethrin.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, the Paecilomyces javanicus bacterium and the composite desinsection host of Imidacloprid of a kind of environmental protection, pollution-free, noresidue is provided.
Another object of the present invention provides the insecticide that is prepared by described desinsection host.
Purpose of the present invention realizes by following technical proposals:
Provide a kind of Paecilomyces javanicus and Imidacloprid composite desinsection host, described host is the mixture of Paecilomyces javanicus and Imidacloprid, and the mass ratio of described Paecilomyces javanicus and Imidacloprid is 100: 0.6～2.5.
In order to realize the present invention better, the mass ratio of described Paecilomyces javanicus and Imidacloprid is preferably 100: 0.7～and 2.0.
Described Paecilomyces javanicus (Isaria javanicus (Friendrichs ﹠amp; Bally)) bacterial strain IJ-N2; belong to hyphomycetales, paecilomyces is done the nature reserve, hilllock from Guangxi province and is separated acquisition by the diamond-back moth polypide of entomogenous fungi natural infection; the bacterial strain preserving number: CCTCC No:M208085, on June 10th, 2007 is in China's typical culture collection center preservation.In being 200810029158.6 patent application, application number has a detailed description.Paecilomyces javanicus bacterium colony on the Cha Shi medium is stratiform, and one deck edge of median rise is irregular, khaki; bacterium colony back side central authorities white, outer rim is faint yellow, and column or ellipse expand in bottle metulae portion; upwards attenuate suddenly, 7.5-12.5 * 2-2.5 μ m, 2-3 wheel is born on the conidiophore.Conidium cylindricality or oblong, 4-6.7 * 1-1.5 μ m is discrete long-chain.
As a kind of preferred, described Paecilomyces javanicus is Paecilomyces javanicus conidial powder or Paecilomyces javanicus spore suspension.
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, as preferred scheme, the present invention provides a kind of insecticide that is prepared by described desinsection host simultaneously, described insecticide is made up of host and assistant agent, host is 12.5～55% by mass percentage, and assistant agent is 45～87.5%.
Described assistant agent is stabilizing agent, auxiliary agent and filler, and by mass percentage, stabilizer comprises compounded pesticides 5～40%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 10～45%.
Described stabilizing agent is one or more mixtures in glycerine, paraffin oil, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, methyl green, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium (NNO); Described filler is talcum powder, kaolin, potter's clay or clay.
The compounded pesticides of described Paecilomyces javanicus and Imidacloprid can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art; have following advantage and beneficial effect: the bacterial strain that (1) the present invention adopts is done the nature reserve, hilllock from Guangxi province and is separated acquisition by the diamond-back moth polypide of entomogenous fungi natural infection; be China domestic bacterial strain; be not from external introduction, can adapt to local natural environment.(2) compounded pesticides of the present invention has determined to obtain the mixed ratio of good insecticidal effect, has reduced the consumption and the persticide residue of Imidacloprid, has adapted to the requirement that organic food is produced, and environmentally safe, noresidue help delaying pest resistance to insecticide and produce.(3) control efficiency to insect of the present invention is better than only using a kind of control efficiency of medicament.Through infecting biological study and indoor bioassay, the compounded pesticides of Paecilomyces javanicus and Imidacloprid has good control efficiency to insects such as red fire ant, prodenia litura, aphid, aleyrodid and diamond-back moths.
Embodiment
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, the present invention is described in further detail below in conjunction with preferred embodiment, but therefore do not limit scope of the present invention.
Following percentage is mass percent, and fungi Imperficti strain is bacterial strain IJ-N2, the bacterial strain preserving number: CCTCC No:M208085, on June 10th, 2007 is in China's typical culture collection center preservation.
Embodiment 1:20% Paecilomyces varioti imidacloprid suspending agent (dilution is 1000 times during use)
A partly is 40% Paecilomyces javanicus conidial suspension: 40% Paecilomyces javanicus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 10%WP Imidacloprid (commercial) 3.0%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 20% Paecilomyces varioti imidacloprid suspending agent, Paecilomyces javanicus: the Imidacloprid mass ratio is 100: 0.75.The virulence test result is as shown in table 1.
Embodiment 2:10% Paecilomyces varioti imidacloprid suspending agent (dilution is 1000 times during use)
A partly is 20% Paecilomyces javanicus conidial suspension: 20% Paecilomyces javanicus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 10%WP Imidacloprid 5.0%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds clay and mends to 100%.
A part and B part respectively account for 50%, directly two parts mixing are promptly obtained 10% Paecilomyces varioti imidacloprid suspending agent, and described Paecilomyces javanicus: the Imidacloprid mass ratio is 100: 2.5.The virulence test result is as shown in table 1.
Embodiment 3:50% Paecilomyces varioti imidacloprid wettable powder
Paecilomyces javanicus bacterium powder (10
10Individual/g) 50%, 10%WP Imidacloprid 3%, saponin 14%, Tween-80 10% adds potter's clay and mends to 100%, makes 50% Paecilomyces varioti imidacloprid wettable powder.Wherein, Paecilomyces javanicus bacterium powder: the Imidacloprid mass ratio is 100: 0.6.The virulence test result is as shown in table 1.
Embodiment 4:50% Paecilomyces varioti imidacloprid oil solution
Paecilomyces javanicus bacterium powder (10
10Individual/g) 50%, 10%WP Imidacloprid 5%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 50% Paecilomyces javanicus imidacloprid oil solution.Wherein, Paecilomyces javanicus bacterium powder: the mass ratio of Imidacloprid is 100: 1.The virulence test result is as shown in table 1.
Embodiment 5:20% Paecilomyces varioti imidacloprid oil solution (dilution is 1000 times during use)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 20%, 10%WP Imidacloprid 2.5%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin are mended to 100%, and mixing promptly obtains 20% Paecilomyces varioti imidacloprid oil solution, Paecilomyces javanicus: the Imidacloprid mass ratio is 100: 1.25.The virulence test result is as shown in table 1.
Embodiment 6:20% Paecilomyces varioti imidacloprid oil solution (dilution is 1000 times during use)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 20%, 10%WP Imidacloprid 4.0%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin are mended to 100%, and mixing promptly obtains 20% Paecilomyces varioti imidacloprid oil solution, Paecilomyces javanicus: the Imidacloprid mass ratio is 100: 2.0.The virulence test result is as shown in table 1.
Embodiment 7:20% Paecilomyces varioti imidacloprid oil solution (dilution is 1000 times during use)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 20%, 10%WP Imidacloprid 1.4%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin are mended to 100%, and mixing promptly obtains 20% Paecilomyces varioti imidacloprid oil solution, Paecilomyces javanicus: the Imidacloprid mass ratio is 100: 0.7.The virulence test result is as shown in table 1.
Embodiment 8: mixture is to the Toxicity Determination of diamond-back moth (Plutella xyostella L.)
During Toxicity Determination, (Brassica campestris L.) makes test plant with cabbage mustard, potted plant seedling is put into dependent insect cage, and access diamond-back moth adult, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, when treating that diamond-back moth on the cabbage mustard blade grows to 2 ages, adopt infusion process to carry out the virulence test, 4 repetitions, 8 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 Paecilomyces javanicus Imidacloprid is to the Toxicity Determination of diamond-back moth
(10
4Spore/ml, μ g/ml) (behind the medicine 8 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Paecilomyces javanicus | y＝0.5920x+2.1039 | 0.9744 | 7.80，3.04-19.95 | 100.00 |
| Imidacloprid (behind the medicine 3 days) | y＝1.5799x+3.0199 | 0.9881 | 17.92，16.25-19.76 | ? |
| Embodiment 1: Paecilomyces javanicus: Imidacloprid (100: 2.5) | y＝0.6089x+2.0482 | 0.9711 | 7.04，2.73-18.20 | 110.80 |
| Embodiment 2: Paecilomyces javanicus: Imidacloprid (100: 0.75) | y＝0.5902x+2.1225 | 0.9638 | 7.50，2.89-19.50 | 104.00 |
| Embodiment 3: Paecilomyces javanicus: Imidacloprid (100: 0.6) | y＝0.5172x+2.6795 | 0.9694 | 3.07，0.77-12.30 | 254.07 |
| Embodiment 4: Paecilomyces javanicus: Imidacloprid (100: 1.0) | y＝0.5713x+2.4515 | 0.9716 | 2.89，1.92-10.72 | 269.90 |
| Embodiment 5: Paecilomyces javanicus: Imidacloprid (100: 1.25) | y＝0.6164x+2.0426 | 0.9800 | 6.28，3.11-16.60 | 124.20 |
| Embodiment 6: Paecilomyces javanicus: Imidacloprid (100: 2.0) | y＝0.6007x+2.6794 | 0.9866 | 7.29，2.84-18.74 | 107.00 |
| Embodiment 7: Paecilomyces javanicus: Imidacloprid (100: 0.70) | y＝0.6370x+2.5187 | 0.9840 | 7.68，3.26-18.96 | 101.56 |
R
2: determination coefficient; TI: relative virus force index.
Claims (7)
1. Paecilomyces javanicus (Isaria javanicus (Friendrichs; Bally)) with the composite desinsection host of Imidacloprid, it is characterized in that described host is the mixture of Paecilomyces javanicus and Imidacloprid, the mass ratio of described Paecilomyces javanicus and Imidacloprid is 100: 0.6～2.5; The bacterial strain preserving number of described fungi Imperficti strain IJ-N2 is CCTCC No:M208085.
2. according to described Paecilomyces javanicus of claim 1 and the composite desinsection host of Imidacloprid, it is characterized in that described Paecilomyces javanicus and Imidacloprid mass ratio are 100: 0.7～2.0.
3. according to described Paecilomyces javanicus of claim 1 and the composite desinsection host of Imidacloprid, it is characterized in that described Paecilomyces javanicus is conidial powder or spore suspension.
4. an insecticide that is prepared by the described desinsection host of claim 1 is characterized in that being made up of host and assistant agent, and host is 12.5～55% by mass percentage, and assistant agent is 45～87.5%.
5. insecticide according to claim 4 is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, stabilizer comprises compounded pesticides 5～40%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 10～45%.
6. insecticide according to claim 5 is characterized in that described stabilizing agent is one or more mixtures in glycerine, paraffin oil, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, methyl green, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium; Described filler is kaolin, potter's clay or clay.
7. according to claim 4,5 or 6 described insecticides, it is characterized in that described insecticide is wetting powder, suspending agent or finish.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101156599A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and imidacloprid |
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| CN101156599A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and imidacloprid |
Non-Patent Citations (2)
| Title |
|---|
| 几种农药对玫烟色拟青霉致病力的影响;黄振等;《华南农业大学学报》;20080731;第29卷(第3期);16-20 * |
| 黄振等.几种农药对玫烟色拟青霉致病力的影响.《华南农业大学学报》.2008,第29卷(第3期),16-20. |
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