CN101513192B - Compound desinsection primary agent and pesticide consisting of verticillium lecanii and abamectin - Google Patents
Compound desinsection primary agent and pesticide consisting of verticillium lecanii and abamectin Download PDFInfo
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- CN101513192B CN101513192B CN2009100377473A CN200910037747A CN101513192B CN 101513192 B CN101513192 B CN 101513192B CN 2009100377473 A CN2009100377473 A CN 2009100377473A CN 200910037747 A CN200910037747 A CN 200910037747A CN 101513192 B CN101513192 B CN 101513192B
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- verticillium lecanii
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- avermectin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a compound desinsection primary agent and a pesticide consisting of verticillium lecanii and abamectin. The primary agent is a mixture of the verticillium lecanii and the abamectin, wherein, the mass ratio between the verticillium lecanii and the abamectin is 100:0.1-0.5; through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pest in particular. The compound pesticide consisting of verticillium lecanii and abamectin of the invention has good effect in preventing and controlling pests such as trialeurodes vaporariorum westwood, bemisa babaci, plant louse, diamond back moth, thrips palmi karny and the like, and has certain effect in controlling mite pests.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, be meant a kind of environmental protection, pollution-free, the Verticillium lecanii of noresidue and abamectin compounded desinsection host and insecticide especially.
Background technology
Verticillium lecanii Verticillium lecanii (Zimmermann) Viegas is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, have features such as the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use Verticillium lecanii has important economic implications.Verticillium lecanii distributes extensively, Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid, thrips etc. is had good control efficiency, has broad application prospects.
Avermectin (Abamectins) is a kind of never poison, act on insect synapse or neuromuscular synapse acceptor, the information transmission of nerve endings has activation to chloride channel in the interference insect body, thus the getting in touch of block nerves tip and muscle.Avermectin has paralysis and food refusal effect to insect, blade there is very strong osmosis, can kill subepidermal insect, and the longevity of residure is long, insecticidal spectrum is wide, the speed of knocking down is fast, be a kind of biogenic pesticide of environmentally safe, be mainly used in insects such as control diamond-back moth, aphid, Americal rice leaf miner, mite class.
Adopt the agriculture chemical compounding technology or mix the usage amount that application technique can reduce chemical pesticide in the production reality, it is to improve the quick-acting of microorganism insecticide and reduce a residual important method of chemical pesticide that the chemical pesticide of employing sub-doses mixes with biopesticide, the optimum compound proportion example of screening Verticillium lecanii and Avermectin, significant.The at present domestic relevant report that still finds no relevant Verticillium lecanii and abamectin compounded insecticide.
Summary of the invention
The objective of the invention is to be to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the Verticillium lecanii of noresidue and abamectin compounded desinsection host are provided.
Another object of the present invention provides the insecticide that is prepared by described desinsection host.
Purpose of the present invention is achieved through the following technical solutions:
A kind of Verticillium lecanii and abamectin compounded desinsection host are provided, and described host is the mixture of Verticillium lecanii and Avermectin, and the mass ratio of described Verticillium lecanii and Avermectin is 100: 0.1～0.5.
In order to realize the present invention better, the mass ratio of described Verticillium lecanii and Avermectin is preferably 100: 0.15～and 0.45.
Described Verticillium lecanii Verticillium lecanii (Zimmermann) Viegas bacterial strain VL-N6, belong to hyphomycetales, Verticillium, be located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou, bacterial strain preserving number: CCTCC No:M-208086, on June 10th, 2008 is in China's typical culture collection center preservation, is to the existing detailed description of Verticillium lecanii in 200810029163.7 the patent application at application number.Described Verticillium lecanii bacteria colony white is to cream-colored, and is thin cotton-shaped, and the back side is colourless to deep mixed yellow.Conidiophore is undeveloped, like nutrient hypha, Dan Sheng is arranged on it, to the bottle stalk of giving birth to or 3～4 verticillate, and the bottle stalk is very thin, and size is because of bacterial strain and cell age is different differs greatly 8.5-40 * 0.8-2.2 μ m; Conidium is oval to cylindrical, two terminal circle, and Dan Sheng is gathered into head at bottle stalk top, and size makes a variation bigger because of bacterial strain reaches, 2.3-10 * 1-2.6 μ m.
As a kind of preferred, described Verticillium lecanii is Verticillium lecanii conidial powder or Verticillium lecanii spore suspension.
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, as preferred scheme, the present invention provides a kind of insecticide that is prepared by described desinsection host simultaneously, described insecticide is made up of host and assistant agent, host is 20～60% by mass percentage, and assistant agent is 40～80%; Described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, 20～23% of the stabilizer comprises insecticide, auxiliary agent account for insecticide 7～22%, filler accounts for 8～35% of insecticide.
Described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures among Tween-80, methyl green, sodium lignin sulfonate, the wetting agent LX; Described filler is kaolin, diatomite or potter's clay.
The compounded pesticides of described Verticillium lecanii and Avermectin can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art has following advantage and beneficial effect:
(1) the bacterial strain of the present invention Bemisia tabaci polypide of being infected from the area, GuangZhou, Guangdong Province separates and obtains, and is China domestic bacterial strain, is not from external introduction, can adapt to domestic natural ecological environment.
(2) the present invention adopts the agricultural chemicals of sub-doses and biopesticide to be mixed, determine to obtain the mixed ratio of good insecticidal effect, reduced the consumption and the persticide residue of Avermectin, adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.
(3) compounded pesticides control efficiency of the present invention is better than only using a kind of control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, the compounded pesticides of Verticillium lecanii and Avermectin has good control efficiency to insects such as Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid, thrips, simultaneously diamond-back moth, mite pest is had certain control effect.
Embodiment
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, the present invention is described in further detail below in conjunction with preferred embodiment, but therefore do not limit scope of the present invention.
Be mass percent described in following examples, described Verticillium lecanii is the VL-N6 bacterial strain, the bacterial strain preserving number: CCTCC No:M-208086, preservation date are on June 10th, 2008, specifically describe to see that application number is in 200810029163.7 the patent application.
Embodiment 1:20% Verticillium lecanii Avermectin suspending agent (dilution is 2000 times during use)
A partly is 40% Verticillium lecanii conidial suspension: 40% Verticillium lecanii conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, and pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 1.8% Avermectin (commercial) 12%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 20% Verticillium lecanii Avermectin suspending agent, Verticillium lecanii: the Avermectin mass ratio is 100: 0.54.The virulence test result is as shown in table 1.
Embodiment 2:50% Verticillium lecanii Avermectin finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, 1.8% Avermectin 10% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Verticillium lecanii Avermectin finish.Wherein, Verticillium lecanii bacterium powder: the Avermectin mass ratio is 100: 0.36.The virulence test result is as shown in table 1.
Embodiment 3:60% Verticillium lecanii Avermectin finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 60%, 1.8% Avermectin 5% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% Verticillium lecanii Avermectin finish.Wherein, Verticillium lecanii bacterium powder: the mass ratio of Avermectin is 100: 0.15.The virulence test result is as shown in table 1.
Embodiment 4:60% Paecilomyces varioti Avermectin finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 60%, 1.8% Avermectin 3% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% Verticillium lecanii Avermectin finish.Wherein, Verticillium lecanii bacterium powder: the mass ratio of Avermectin is 100: 0.09.The virulence test result is as shown in table 1.
Embodiment 5:50% Verticillium lecanii Avermectin finish (dilution is 2000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, 1.8% Avermectin 12.5% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Verticillium lecanii Avermectin finish.Wherein, Verticillium lecanii bacterium powder: the Avermectin mass ratio is 100: 0.45.The virulence test result is as shown in table 1.
Embodiment 7: to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat behind its 12h of laying eggs it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
The composite Toxicity Determination of table 1 Verticillium lecanii and Avermectin to Bemisia tabaci
(10
4Spore/ml, μ g/ml) (behind the medicine 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Verticillium lecanii | y＝0.3744x+3.3240 | 0.9814 | 2.99，0.98-9.16 | 100.00 |
| Avermectin (behind the medicine 2 days) | y＝1.5099x+5.1873 | 0.9849 | 0.75，0.46-1.22 | |
| Example 1: Verticillium lecanii: Avermectin (100: 0.50) | y＝0.2854x+4.0244 | 0.9889 | 2.62，0.32-21.62 | 114.12 |
| Example 2: Verticillium lecanii: Avermectin (100: 0.36) | y＝0.2798x+4.0728 | 0.9717 | 2.05，0.22-18.83 | 145.85 |
| Example 3: Verticillium lecanii: Avermectin (100: 0.15) | y＝0.2842x+4.1406 | 0.9977 | 1.06，0.92-10.52 | 282.08 |
| Example 4: Verticillium lecanii: Avermectin (100: 0.09) | y＝0.2771x+4.1130 | 0.9898 | 1.87，0.18-18.80 | 159.89 |
| Example 5: Verticillium lecanii: Avermectin (100: 0.45) | y＝0.2918x+4.0398 | 0.9884 | 1.95，0.23-16.86 | 153.33 |
R
2: determination coefficient; TI: relative virus force index.
Claims (5)
1. a Verticillium lecanii (Verticillium lecanii (Zimmermann) Viegas) and abamectin compounded desinsection host, it is characterized in that described host is the mixture of Verticillium lecanii and Avermectin, the mass ratio of described Verticillium lecanii and Avermectin is 100: 0.15～0.45; The bacterial strain preserving number of described verticillium lecanii strain VL-N6 is CCTCC No:M-208086.
2. insecticide that is prepared by the described desinsection host of claim 1 is characterized in that described insecticide is made up of host and assistant agent, and host is 20～60% by mass percentage, and assistant agent is 40～80%.
3. insecticide according to claim 2 is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, 20～23% of the stabilizer comprises insecticide, auxiliary agent account for insecticide 7～22%, filler accounts for 8～35% of insecticide.
4. insecticide according to claim 3 is characterized in that described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures in Tween-80, methyl green, the sodium lignin sulfonate; Described filler is kaolin, diatomite or potter's clay.
5. according to claim 2,3 or 4 described insecticides, it is characterized in that described insecticide is wetting powder, suspending agent or finish.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100377473A CN101513192B (en) | 2009-03-10 | 2009-03-10 | Compound desinsection primary agent and pesticide consisting of verticillium lecanii and abamectin |
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| CN2009100377473A CN101513192B (en) | 2009-03-10 | 2009-03-10 | Compound desinsection primary agent and pesticide consisting of verticillium lecanii and abamectin |
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| CN101513192B true CN101513192B (en) | 2011-11-16 |
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| CN105274022B (en) * | 2015-07-27 | 2018-06-19 | 华南农业大学 | One plant of knife spore Verticillium dahliae LpTS01 and its application in Bemisia tabaci is prevented |
| CN106577776A (en) * | 2016-11-25 | 2017-04-26 | 河池市农业科学研究所 | Microbial pesticide for treating corn diseases |
| CN112602728A (en) * | 2020-11-26 | 2021-04-06 | 赞皇县龙堂苑农业科技有限责任公司 | Compound preparation of verticillium lecanii and emamectin benzoate |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101319193A (en) * | 2008-07-01 | 2008-12-10 | 华南农业大学 | Verticillium lecanii strain and uses thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101319193A (en) * | 2008-07-01 | 2008-12-10 | 华南农业大学 | Verticillium lecanii strain and uses thereof |
Non-Patent Citations (2)
| Title |
|---|
| 孔琼,等.蜡蚧轮枝菌与农药混配防治甘蓝蚜的研究.《安徽农业科学》.2007,第35卷(第1期),第145-146,167页. * |
| 肖燕,等.应用虫生真菌防治烟粉虱的现状.《昆虫天敌》.2001,第23卷(第1期),第30-34页. * |
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