CN101273728B - Pesticides combined with Paecilomyces fumosoroseus and Azadirachtin - Google Patents
Pesticides combined with Paecilomyces fumosoroseus and Azadirachtin Download PDFInfo
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- CN101273728B CN101273728B CN2008100275865A CN200810027586A CN101273728B CN 101273728 B CN101273728 B CN 101273728B CN 2008100275865 A CN2008100275865 A CN 2008100275865A CN 200810027586 A CN200810027586 A CN 200810027586A CN 101273728 B CN101273728 B CN 101273728B
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- paecilomyces fumosoroseus
- nimbin
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Abstract
The invention discloses a combination pesticide of paecilomyces fumosoroseus and azadirachtin, comprising 20 to 60 percent of host crystal and 40 to 80 percent of adjuvant according to mass percent, the host crystal is the mixture of paecilomyces fumosoroseus and azadirachtin, the mass ratio of the paecilomyces fumosoroseus and the azadirachtin is 100: 0.05 to 0.3; the adjuvant is stabilizer, addition agent and filler. By adopting the blending of agricultural chemical and biopesticide with sub-dose, the combination pesticide of the invention is superior in control effect when only one medicine is used, meets the production demands of organic foods, has no pollution and residue to environment, and is beneficial to deferring the generation of drug fastness of pests, and has good control effects on pests such as whitefly and aphis and diamondback moth, etc.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, be meant a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of nimbin especially.
Background technology
Paecilomyces fumosoroseus (Paecilomyces fumosoroseus (Wize) Brown﹠amp; Smith) be a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use paecilomyces fumosoroseus has important economic implications.Paecilomyces fumosoroseus distributes extensively, aphid, Bemisia tabaci, diamond-back moth etc. is had good control efficiency, has a good application prospect.
Nimbin (azadirachtin) is acknowledged as the most important effect that insect is had food refusal, stomach toxicity, tags and suppress to grow in the whole world, and its mechanism of action mainly is the internal secretion of upsetting insect.Action target is cerebral nerve secretory cell, corpora cardiaca, prothoracic gland etc., influence promotes the synthetic of prothoracic gland hormone and discharges, lower prothoracic gland and the induction of prothoracic gland hormone is caused the 20-hydroxyecdysone is synthetic, the deficiency of secretion, cause the insectean metamorphosis impaired development.The nimbin preparation shows different physiologically actives to kind of insect surplus 400.Have advantages such as higher mammal low toxicity, low-residual, insect are difficult for developing immunity to drugs, but the resource-constrained of nimbin, the supply of nimbin preparation is far below the demand in producing.
Adopt the agriculture chemical compounding technology or mix the usage amount that application technique can reduce nimbin in the production reality, the applicant mixes the quick-acting that can improve microorganism insecticide simultaneously and reduces residue of pesticide by a large amount of agricultural chemicals that experimental studies have found that sub-doses with biopesticide, and does not find the correlation technique report of the compounded pesticides of paecilomyces fumosoroseus and nimbin at present as yet.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of nimbin are provided.
Purpose of the present invention realizes by following technical proposals: the compounded pesticides of a kind of paecilomyces fumosoroseus and nimbin is provided, is made up of host and assistant agent, host is 20～60% by mass percentage, and assistant agent is 40～80%; Described host is the mixture of paecilomyces fumosoroseus and nimbin; Described assistant agent is stabilizing agent, auxiliary agent and filler.
Described paecilomyces fumosoroseus Paecilomyces fumosoroseus (Wize) Brown﹠amp; Smith bacterial strain PF01-N10 belongs to hyphomycetales, and paecilomyces is located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou the bacterial strain preserving number: CCTCC No:M-207088, on June 27th, 2007 is in China's typical culture collection center preservation.The Paecilomyces Fumosoroseus back side that falls is colourless or yellow, and the conidiophore list is given birth to or is gathered into coremium, 100 * 1.5～2 μ m, and wall is smooth, and is transparent, forms by giving birth to the colyliform branch that 4～6 bottles stalks form verticil mostly.Bottle metulae portion sphere, or intend ellipse and expand top elongated, 5.7～8 * 1～2 μ m.The conidium cylindricality is to fusiformis, and is smooth, is clear to little pale red, 3～4 * 1～2 μ m, no chlamydospore.
As preferred version of the present invention, the mass ratio of described paecilomyces fumosoroseus and nimbin is 100: 0.05～0.3, and the mass ratio that is preferably paecilomyces fumosoroseus and nimbin especially is 100: 0.08～0.25.
Preferred paecilomyces fumosoroseus conidial powder of described paecilomyces fumosoroseus or paecilomyces fumosoroseus spore suspension.
Described stabilizing agent, auxiliary agent and filler by mass percentage, stabilizer comprises compounded pesticides 23～30%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for compounded pesticides 10～35%.
Phosphate buffer or the paraffin oil of described stabilizing agent preferably glycerine, 0.21M; A kind of or any two or more mixture among the preferred Tween-80 of described auxiliary agent, methyl green, sodium lignin sulfonate, the wetting agent LX; The preferred kaolin of described filler, diatomite or potter's clay
The compounded pesticides of described paecilomyces fumosoroseus and nimbin can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art, have following advantage and beneficial effect: the Bemisia tabaci polypide that the Paecilomyces Fumosoroseus strain that (1) the present invention adopts is infected from the area, Guangzhou, Guangdong Province, China separates acquisition, be China domestic bacterial strain, be not from external introduction, can adapt to the local natural environment of China.(2) the present invention adopts the agricultural chemicals of sub-doses and biopesticide to be mixed, and adapts to the requirement that organic food is produced, and environmentally safe, noresidue help delaying pest resistance to insecticide and produce.(3) insecticide control effect of the present invention is better than only using a kind of control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, paecilomyces fumosoroseus has the very strong insecticidal effect that infects to various pests especially sucking pest.The compounded pesticides of paecilomyces fumosoroseus and nimbin has good control efficiency to insects such as aleyrodid, aphid, diamond-back moths.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, the present invention is through a large amount of screenings, search out paecilomyces fumosoroseus, and sum up suitable mixed ratio, stabilizing agent, auxiliary agent and filler can use with reference to this area is conventional, the all proportions scheme can not be given unnecessary details one by one among the embodiment, but therefore the present invention is not defined among the following embodiment.
Following percentage is mass percent, and the Paecilomyces Fumosoroseus strain is PF01-N10, the bacterial strain preserving number: CCTCC No:M-207088, preservation date on June 27th, 2007.
Embodiment 1:20% Paecilomyces varioti nimbin suspending agent (diluting 2000 times)
A partly is 40% paecilomyces fumosoroseus conidial suspension: 40% paecilomyces fumosoroseus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, and pH7.0 is in 4～8 ℃ of preservations.
B partly is a wetting powder: nimbin (commercial) 0.1%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 20% Paecilomyces varioti nimbin suspending agent, paecilomyces fumosoroseus: the nimbin mass ratio is 100: 0.25.The virulence test result is as shown in table 1.
Embodiment 2:50% paecilomyces fumosoroseus nimbin finish (diluting 2000 times)
Paecilomyces Fumosoroseus powder (10
10Individual/g) 50%, nimbin 0.1% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Paecilomyces varioti nimbin finish.Wherein, Paecilomyces Fumosoroseus powder: the nimbin mass ratio is 100: 0.2.The virulence test result is as shown in table 1.
Embodiment 3:60% Paecilomyces varioti nimbin finish (diluting 2000 times)
Paecilomyces Fumosoroseus powder (10
10Individual/g) 60%, nimbin 0.05% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% paecilomyces fumosoroseus nimbin finish.Wherein, Paecilomyces Fumosoroseus powder: the mass ratio of nimbin is 100: 0.083.The virulence test result is as shown in table 1.
Embodiment 4:50% Paecilomyces varioti nimbin wetting powder (diluting 2000 times)
Paecilomyces Fumosoroseus powder (10
10Individual/g) 50%, nimbin 0.1% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% paecilomyces fumosoroseus nimbin wetting powder.Wherein, Paecilomyces Fumosoroseus powder: the mass ratio of nimbin is 100: 0.2.The virulence test result is as shown in table 1.
Embodiment 5:20% Paecilomyces varioti nimbin suspending agent (diluting 2000 times)
A partly is 40% paecilomyces fumosoroseus conidial suspension: 40% paecilomyces fumosoroseus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, and pH7.0 is in 4～8 ℃ of preservations.
B partly is a wetting powder: nimbin (commercial) 0.2%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 20% Paecilomyces varioti nimbin suspending agent, paecilomyces fumosoroseus: the nimbin mass ratio is 100: 0.5.The virulence test result is as shown in table 1.
Embodiment 6:60% Paecilomyces varioti nimbin finish (diluting 2000 times)
The Paecilomyces Fumosoroseus powder (1010/g) 60%, nimbin 0.025% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% paecilomyces fumosoroseus nimbin finish.Wherein, Paecilomyces Fumosoroseus powder: the mass ratio of nimbin is 100: 0.04.The virulence test result is as shown in table 1.
Embodiment 7: mixture is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm one lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its experimental result is as shown in table 1.
Table 1 paecilomyces fumosoroseus nimbin is to the Toxicity Determination (10 of Bemisia tabaci
3Spore/ml, μ g/ml) (after the administration 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Paecilomyces fumosoroseus | y＝0.34x+3.72 | 0.9617 | 6.11，3.08-12.11 | 100.00 |
| Nimbin (behind the medicine 2 days) | y＝1.5099x +5.1873 | 0.9849 | 0.75，0.46-1.22 | |
| Paecilomyces fumosoroseus: nimbin (100: 0.25) | y＝0.5650x+ 2.9860 | 0.9723 | 3.72，0.34-38.68 | 164.25 |
| Paecilomyces fumosoroseus: nimbin (100: 0.2) | y＝0.5768x+ 2.9027 | 0.9825 | 4.49，0.47-40.20 | 136.08 |
| Paecilomyces fumosoroseus: nimbin (100: 0.083) | y＝0.5884x+ 2.8267 | 0.9711 | 4.94，0.59-41.639 | 123.68 |
| Paecilomyces fumosoroseus: nimbin (100: 0.2) | y＝0.5747x+ 2.9281 | 0.9842 | 4.03，0.42-38.77 | 151.61 |
| Paecilomyces fumosoroseus: nimbin (100: 0.04) | y＝0.4877x+ 3.2016 | 0.9514 | 4.87，0.47-50.00 | 125.46 |
| Paecilomyces fumosoroseus: nimbin (100: 0.5) | y＝0.4805x+ 3.3188 | 0.9468 | 3.15，0.23-43.27 | 193.97 |
R
2: determination coefficient; TI: relative virus force index.
Claims (7)
1. the compounded pesticides of a paecilomyces fumosoroseus (Paecilomyces fumosoroseus) and nimbin is made up of host and assistant agent, it is characterized in that host is 20～60% by mass percentage, and assistant agent is 40～80%; Described host is the mixture of paecilomyces fumosoroseus and nimbin; Described assistant agent is stabilizing agent, auxiliary agent and filler; Described Paecilomyces Fumosoroseus strain PF01-N10 preserving number is CCTCC No:M-207088; Described paecilomyces fumosoroseus and nimbin mass ratio are 100: 0.05～0.3; Described auxiliary agent is a kind of or any two or more mixture in Tween-80, methyl green or the sodium lignin sulfonate.
2. compounded pesticides according to claim 1 is characterized in that described paecilomyces fumosoroseus and nimbin mass ratio are 100: 0.08～0.25.
3. compounded pesticides according to claim 1 and 2 is characterized in that described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or paecilomyces fumosoroseus spore suspension.
4. compounded pesticides according to claim 1 is characterized in that described stabilizing agent, auxiliary agent and filler by mass percentage, and stabilizer comprises compounded pesticides 23～30%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for compounded pesticides 10～35%.
5. according to claim 1 or 4 described compounded pesticides, it is characterized in that described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M.
6. according to claim 1 or 4 described compounded pesticides, it is characterized in that described filler is kaolin, diatomite or potter's clay.
7. compounded pesticides according to claim 1 is characterized in that described compounded pesticides is wetting powder, suspending agent or finish.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008100275865A CN101273728B (en) | 2008-04-22 | 2008-04-22 | Pesticides combined with Paecilomyces fumosoroseus and Azadirachtin |
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| CN2008100275865A CN101273728B (en) | 2008-04-22 | 2008-04-22 | Pesticides combined with Paecilomyces fumosoroseus and Azadirachtin |
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| CN101273728B true CN101273728B (en) | 2011-01-19 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101473849B (en) * | 2009-01-17 | 2012-01-04 | 邹一平 | Application of paraffin oil composition in preventing and treating diseases and pests of plant |
| CN101810192A (en) * | 2010-03-11 | 2010-08-25 | 徐建军 | Pesticide mixing method capable of reducing pesticide residue in agricultural products |
| MX364353B (en) | 2011-04-29 | 2017-06-13 | Inst De Ecologia A C Star | Uses, methods and biological compositions of the paecilomyces genus for the control, prevention and eradication of phytoparasites in solanaceae crops. |
| CN104222161B (en) * | 2014-08-29 | 2016-08-24 | 广东省农业科学院植物保护研究所 | Insecticides and its preparation method and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1640256A (en) * | 2004-01-13 | 2005-07-20 | 西安近代化学研究所 | Mixed biological pesticide containing nimbin |
| CN101156600A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and aphox |
| CN101156599A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and imidacloprid |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1640256A (en) * | 2004-01-13 | 2005-07-20 | 西安近代化学研究所 | Mixed biological pesticide containing nimbin |
| CN101156600A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and aphox |
| CN101156599A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and imidacloprid |
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