CN101273725B - Pesticides combined with Aschersonia aleyrodis and Acetamiprid - Google Patents
Pesticides combined with Aschersonia aleyrodis and Acetamiprid Download PDFInfo
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- CN101273725B CN101273725B CN2008100275831A CN200810027583A CN101273725B CN 101273725 B CN101273725 B CN 101273725B CN 2008100275831 A CN2008100275831 A CN 2008100275831A CN 200810027583 A CN200810027583 A CN 200810027583A CN 101273725 B CN101273725 B CN 101273725B
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- acetamiprid
- aschersonia aleyrodis
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Abstract
The invention discloses a combination pesticide of aschersonia aleyrodis webber and acetamiprid, comprising 32 to 61 percent of host crystal and 39 to 68 percent of adjuvant according to mass percent, the host crystal is the mixture of aschersonia aleyrodis webber and acetamiprid, the mass ratio of the aschersonia aleyrodis webber and the acetamiprid is 100: 1.67 to 6.67; the adjuvant is stabilizer, addition agent and filler. The invention discloses that the combination pesticide of aschersonia aleyrodis webber and acetamiprid is processed into wettable powder, suspending agent or medicinal oil. After long-term infection biology research and indoor biological assay, the aschersonia aleyrodis webber of the pesticide of the invention has rather strong infection disinsection effect on various pests, especially has good control effects on pests such as whitefly and aphis, etc.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, be meant a kind of environmental protection, pollution-free, the Aschersonia aleyrodis of noresidue and the compounded pesticides of Acetamiprid especially.
Background technology
Aschersonia aleyrodis (Aschersonia aleyrodis Webber) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that Bt is developed immunity to drugs of control, the development and use Aschersonia aleyrodis has important economic implications.Aschersonia aleyrodis is distributed more widely, and sucking mouth partss such as aphid, Bemisia tabaci are had good control efficiency, has a good application prospect.
The novel nicotinamide insecticide of insecticidal actions such as Acetamiprid (acetamiprid) is that having of using at present tagged, stomach toxicity, infiltration and interior suction, act on the nAChR of insect nervous system postsynaptic membrane, and nerve on every side, make insect keep excited, paralysis death then.Be mainly used in suckings pest such as control homoptera pest such as leafhopper, aleyrodid, aphid, lepidoptera pest such as diamond-back moth, peach fruit borer etc., coleopteran pest such as longicorn, thrips insect such as thrips etc.; Have and good kill ovum, larvicidal activity, but it is not high that non-target pest is shown as activity, it is certain residual to use the back can detect on fruits and vegetables simultaneously.Adopting the agriculture chemical compounding technology or mixing application technique is that to overcome insecticidal spectrum narrow or delay a drug-fast important method, and adopting the agricultural chemicals of sub-doses to mix with biopesticide is to improve the quick-acting of microorganism insecticide and an important method of minimizing residue of pesticide.Therefore, the Compositional type insecticide of exploitation Acetamiprid is significant.Do not find at present the correlation technique report of the compounded pesticides of Aschersonia aleyrodis and Acetamiprid as yet.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the Aschersonia aleyrodis of noresidue and the compounded pesticides of Acetamiprid are provided.
Purpose of the present invention is achieved through the following technical solutions: the compounded pesticides of a kind of Aschersonia aleyrodis and Acetamiprid being provided, being made up of host and assistant agent, is host 32～61% by mass percentage, assistant agent 39～68%; Described host is the mixture of Aschersonia aleyrodis and Acetamiprid; Described assistant agent is stabilizing agent, auxiliary agent and filler.
Described Aschersonia aleyrodis (Aschersonia aleyrodis Webber) belongs to Coelomycetes, and seat shell spore belongs to, and is located away from the citrus whitefly polypide of being infected in the area, Guangzhou.The bacterial strain preserving number: CCTCC No:M-207087, on June 27th, 2007 is in China's typical culture collection center preservation.Flat cushion of Aschersonia aleyrodis stroma or peltate, little by hair, white is to glassy yellow, diameter 1.0～1.5mm, high 0.4～0.6mm, surrounded by basic unit, basic unit is translucent, white, wide 0.2～0.5mm, forming by close the knitting of mycelia. mycelia is expanded sometimes, and is transparent, and wall is smooth, generous 0.2～6.4 μ m, wall thickness, 0.8～2.2 μ m, pycnidium embeds stroma, and is avette or oblate spheroid is irregular, 120～280 * 150～320 μ m, the aperture of opening wide is arranged, circle, the aperture is covered by orange pionnote.The conidium spindle, crooked sometimes, the top point, monospore, transparent, wall is smooth.The conidiophore list is given birth to or is two verticillate, 2～5 tubular verticils of tool.Verticil is thinner, and is slim and frahile, carefully cylindrical, and do not hold level with both hands and cut, 7.8～17.5 μ m * 1.2～1.8 μ m, lateral filament bears from conidiophore, and is thread, transparent, long 30～100 μ m (rare 350 μ m), wide 1.0～1.6 μ m.
As preferred version of the present invention, the mass ratio of described Aschersonia aleyrodis and Acetamiprid is an Aschersonia aleyrodis: Acetamiprid=100: 1.5～10, the mass ratio of particularly preferred Aschersonia aleyrodis and Acetamiprid is an Aschersonia aleyrodis: Acetamiprid=100: 1.67～6.67.
Preferred Aschersonia aleyrodis conidial powder of described Aschersonia aleyrodis or Aschersonia aleyrodis spore suspension.
Described stabilizing agent, auxiliary agent and filler by mass percentage, be respectively the stabilizer comprises compounded pesticides 20～23%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for 18～33% of compounded pesticides.
Phosphate buffer or the paraffin oil of described stabilizing agent preferably glycerine, 0.21M; A kind of or any two or more mixture among the preferred Tween-80 of described auxiliary agent, methyl green, sodium lignin sulfonate, the wetting agent LX; The preferred kaolin of described filler, diatomite or potter's clay.
The compounded pesticides of described Aschersonia aleyrodis and Acetamiprid can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art, have following advantage and beneficial effect: the citrus whitefly polypide that the lice seat shell spore bacterial strain that (1) the present invention adopts is infected from the area, Guangzhou, Guangdong Province, China separates acquisition, be China domestic bacterial strain, can adapt to local natural environment.(2) the present invention adopts the agricultural chemicals of sub-doses to mix with biopesticide, has reduced Acetamiprid and has used the residue problem of back on fruits and vegetables, has adapted to the requirement that organic food is produced, and environmentally safe, noresidue help delaying pest resistance to insecticide and produce.(3) the present invention is directed to Acetamiprid, that non-target pest is shown as activity is not high, adopts suitable biopesticide to be mixed, and control efficiency is better than only being used alone the control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, Aschersonia aleyrodis has the very strong insecticidal effect that infects to various pests especially sucking pest.The present invention has good control efficiency to insects such as aleyrodid, aphids for composite dose.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, the present invention searches out Aschersonia aleyrodis as the biopesticide bacterial strain, and sums up suitable mixed ratio through a large amount of screenings, the embodiment that below provides is a typical scenario of the present invention, does not therefore limit the present invention.Described stabilizing agent, auxiliary agent and filler can add according to this area conventional method and ratio, do not give unnecessary details in an embodiment one by one.
Following percentage is mass percent, and the Aschersonia aleyrodis bacterial strain is AA01-N8, the bacterial strain preserving number: CCTCC No:M-207087, on June 27th, 2007 is in China's typical culture collection center preservation.
Embodiment 1:30% Aschersonia aleyrodis acetamiprid suspending agent (diluting 2000 times)
A partly is 60% Aschersonia aleyrodis conidial suspension: with 60% Aschersonia aleyrodis conidial powder, be dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M pH7.0.In 4～8 ℃ of preservations.
B partly is a wetting powder: Acetamiprid (commercial) 4%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and directly with two parts mixing, promptly obtain 30% Aschersonia aleyrodis acetamiprid suspending agent, Aschersonia aleyrodis: the Acetamiprid mass ratio is 100: 6.67.The virulence test result is as shown in table 1.
Embodiment 2:50% Aschersonia aleyrodis Acetamiprid finish (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 50%, Acetamiprid 2% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Aschersonia aleyrodis Acetamiprid finish.Wherein, Aschersonia aleyrodis: the Acetamiprid mass ratio is 100: 4.The virulence test result is as shown in table 1.
Embodiment 3:60% Aschersonia aleyrodis Acetamiprid wetting powder (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 60%, Acetamiprid 1% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 3%, wetting agent LX 2%, kaolin is mended to 100%, makes 60% Aschersonia aleyrodis Acetamiprid wetting powder.Wherein, Aschersonia aleyrodis powder: the mass ratio of Acetamiprid is 100: 1.67.The virulence test result is as shown in table 1.
Embodiment 4:50% Aschersonia aleyrodis Acetamiprid wetting powder (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 50%, Acetamiprid 2% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% Aschersonia aleyrodis Acetamiprid wetting powder.Wherein, Aschersonia aleyrodis powder: the mass ratio of Acetamiprid is 100: 4.The virulence test result is as shown in table 1.
Embodiment 5:50% Aschersonia aleyrodis Acetamiprid wetting powder (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 50%, Acetamiprid 0.5% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% Aschersonia aleyrodis Acetamiprid wetting powder.Wherein, Aschersonia aleyrodis powder: the mass ratio of Acetamiprid is 100: 1.0.The virulence test result is as shown in table 1.
Embodiment 6:30% Aschersonia aleyrodis acetamiprid suspending agent (diluting 2000 times)
A partly is 60% Aschersonia aleyrodis conidial suspension: with 60% Aschersonia aleyrodis conidial powder, be dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M pH7.0.In 4～8 ℃ of preservations.
B partly is a wetting powder: Acetamiprid (commercial) 6%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and directly with two parts mixing, promptly obtain 30% Aschersonia aleyrodis acetamiprid suspending agent, Aschersonia aleyrodis: the Acetamiprid mass ratio is 100: 10.The virulence test result is as shown in table 1.
Embodiment 7: compounded pesticides is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC50 then, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun.Its result of the test such as table 1.
Table 1 Aschersonia aleyrodis Acetamiprid is to the Toxicity Determination (10 of Bemisia tabaci
4Spore/ml, μ g/ml) (behind the medicine 12 days)
| Handle | Virulence regression equation | R 2 | LC50 and 95% confidence interval | TI |
| Aschersonia aleyrodis | y＝0.9082x+0.9004 | 0.9321 | 3.27，1.56-6.85 | 100.00 |
| Acetamiprid (behind the medicine 3 days) | y＝1.6004x+3.3185 | 0.9700 | 11.24，9.14-13.81 | |
| Aschersonia aleyrodis: Acetamiprid (100: 6.67) | y＝0.7147x+1.9817 | 0.9935 | 1.67，0.44-6.32 | 195.81 |
| Aschersonia aleyrodis: Acetamiprid (100: 4) | y＝0.6345x+2.5083 | 0.9679 | 0.84，0.15-4.92 | 389.28 |
| Aschersonia aleyrodis: Acetamiprid (100: 1.67) | y＝0.6629x+2.3280 | 0.9767 | 1.07，0.22-5.30 | 305.61 |
| Aschersonia aleyrodis: Acetamiprid (100: 4) | y＝0.6304x+2.5285 | 0.9761 | 0.82，0.14-4.93 | 435.71 |
| Aschersonia aleyrodis: Acetamiprid (100: 1.0) | y＝0.6018x+2.5605 | 0.9529 | 1.13，0.22-5.81 | 289.38 |
| Aschersonia aleyrodis: Acetamiprid (100: 10) | y＝0.6057x+2.51165 | 0.9714 | 1.28，0.26-6.25 | 255.47 |
R2: determination coefficient; TI: relative virus force index.
Claims (8)
1. the compounded pesticides of an Aschersonia aleyrodis (Aschersonia aleyrodis Webber) and Acetamiprid is made up of host and assistant agent, it is characterized in that host is 32～61% by mass percentage, and assistant agent is 39～68%; Described host is that Aschersonia aleyrodis and Acetamiprid are 100: 1.5～10 mixtures that mix according to mass ratio; Described assistant agent is stabilizing agent, auxiliary agent and filler.
2. compounded pesticides according to claim 1, the mass ratio that it is characterized in that described Aschersonia aleyrodis and Acetamiprid is 100: 1.67～6.67.
3. compounded pesticides according to claim 1 and 2 is characterized in that described Aschersonia aleyrodis is Aschersonia aleyrodis conidial powder or Aschersonia aleyrodis spore suspension.
4. compounded pesticides according to claim 1, it is characterized in that described stabilizing agent, auxiliary agent and filler by mass percentage, be respectively the stabilizer comprises compounded pesticides 20～23%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for 18～33% of compounded pesticides.
5. according to claim 1 or 4 described compounded pesticides, it is characterized in that described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M.
6. according to claim 1 or 4 described compounded pesticides, it is characterized in that described auxiliary agent is a kind of or any two or more mixture among Tween-80, methyl green, sodium lignin sulfonate, the wetting agent LX.
7. according to claim 1 or 4 described compounded pesticides, it is characterized in that described filler is kaolin, diatomite or potter's clay.
8. compounded pesticides according to claim 1 is characterized in that described compounded pesticides is wetting powder, suspending agent or finish.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008100275831A CN101273725B (en) | 2008-04-22 | 2008-04-22 | Pesticides combined with Aschersonia aleyrodis and Acetamiprid |
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| CN2008100275831A CN101273725B (en) | 2008-04-22 | 2008-04-22 | Pesticides combined with Aschersonia aleyrodis and Acetamiprid |
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| CN101273725A CN101273725A (en) | 2008-10-01 |
| CN101273725B true CN101273725B (en) | 2010-08-18 |
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