CN101317578B - Mixed insecticide of aschersonia aleyrodis webb. and avermectin - Google Patents
Mixed insecticide of aschersonia aleyrodis webb. and avermectin Download PDFInfo
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- CN101317578B CN101317578B CN2008100292502A CN200810029250A CN101317578B CN 101317578 B CN101317578 B CN 101317578B CN 2008100292502 A CN2008100292502 A CN 2008100292502A CN 200810029250 A CN200810029250 A CN 200810029250A CN 101317578 B CN101317578 B CN 101317578B
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- avermectin
- aschersonia aleyrodis
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- aleyrodis
- aschersonia
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses an insecticide compounded by aschersonia aleyrodis and avermectin, which consists of a main agent and an auxiliary agent. According to the mass percentage, the main agent occupies 20-60% and the auxiliary agent occupies 40-80%; the main agent is the mixture of the aschersonia aleyrodis and the avermectin with the mass proportion of 100:0.1-0.4; the auxiliary agent is stabilizer, assisting agent and filling material. The compounded insecticide of the invention can be processed into wettable powder, suspension agent or oil agent; according to long-term infection biological study and indoor biological testing, the aschersonia aleyrodis has extremely strong infection insecticidal effects on a plurality of pests, especially for the pests with piercing-sucking mouthparts. The compounded agent of the invention has excellent control effects on the pests such as whitefly, aphid, etc.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of biological insecticides, be meant a kind of environmental protection, pollution-free, the Aschersonia aleyrodis of noresidue and the compounded pesticides of Avermectin especially.
Background technology
Aschersonia aleyrodis (Aschersonia aleyrodis Webber) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, have characteristics such as the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that Bt is developed immunity to drugs of control, the development and use Aschersonia aleyrodis has important economic implications.Aschersonia aleyrodis is distributed more widely, and sucking mouth partss such as aphid, Bemisia tabaci are had good control efficiency, has a good application prospect.
Avermectin (Abamectins) is a kind of never poison, act on insect synapse or neuromuscular synapse acceptor, the information transmission of nerve endings has activation to chloride channel in the interference insect body, thus the getting in touch of block nerves tip and muscle.Have paralysis and food refusal effect, blade is had very strong osmosis, can kill subepidermal insect, and the longevity of residure is long.Insecticidal spectrum is wide, the speed of knocking down is fast, the biogenic pesticide of environmentally safe.
Be mainly used in insects such as control aleyrodid, aphid, thrips, but along with Avermectin is used historical prolongation, insect to the pesticide resistance of Avermectin developed into demand side to the major issue that solves.It is an important method that improves the quick-acting of microorganism insecticide and reduce residue of pesticide that the chemical pesticide of employing sub-doses mixes with biopesticide, therefore, the Compositional type insecticide of exploitation Avermectin, and seek effective mixed ratio, will have great importance.Still find no at present the relevant Aschersonia aleyrodis and the compounded pesticides of Avermectin.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the Aschersonia aleyrodis of noresidue and the compounded pesticides of Avermectin are provided.
Purpose of the present invention is achieved through the following technical solutions: Aschersonia aleyrodis that is provided and abamectin compounded insecticide are made up of host and assistant agent, and host is 20～60% by mass percentage, and assistant agent is 40～80%; Described host is the mixture of Aschersonia aleyrodis and Avermectin, Aschersonia aleyrodis: the Avermectin mass ratio is 100: 0.1～0.4.
In order to realize the present invention better, the mass ratio of described Aschersonia aleyrodis and Avermectin is an Aschersonia aleyrodis: Avermectin=100: 0.15～0.36.
Described Aschersonia aleyrodis is Aschersonia aleyrodis conidial powder or Aschersonia aleyrodis spore suspension.
Described Aschersonia aleyrodis (Aschersonia aleyrodis Webber) belongs to Coelomycetes, and seat shell spore belongs to, and is located away from the citrus whitefly polypide of being infected in the area, Guangzhou.The bacterial strain preserving number: CCTCC No:M-207087, on June 27th, 2007 is in China's typical culture collection center preservation, and number of patent application is to disclose in 200710031516.2 the patent application.Flat cushion of Aschersonia aleyrodis stroma or peltate, little by hair, white is to glassy yellow, diameter 1.0～1.5mm, high 0.4～0.6mm, surrounded by basic unit, basic unit is translucent, white, wide 0.2～0.5mm is formed by close the knitting of mycelia. and mycelia is expanded sometimes, and is transparent, wall is smooth, generous 0.2～6.4 μ m, wall thickness 0.8～2.2 μ m, pycnidium embeds stroma, and is avette or oblate spheroid is irregular, 120～280 * 150～320 μ m, the aperture of opening wide is arranged, circle, the aperture is covered by orange pionnote.The conidium spindle, crooked sometimes, the top point, monospore, transparent, wall is smooth.The conidiophore list is given birth to or is two verticillate, 2～5 tubular verticils of tool.Verticil is thinner, and is slim and frahile, carefully cylindrical, do not hold level with both hands and cuts 7.8～17.5 μ m * 1.2～1.8 μ m, and lateral filament bears from conidiophore, and is thread, transparent, long 30～100 μ m (rare 350 μ m), wide 1.0～1.6 μ m.
Described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, 20～23% of the stabilizer comprises compounded pesticides, auxiliary agent account for compounded pesticides 7～22%, filler accounts for 5～30% of compounded pesticides.
Described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures among Tween-80, methyl green, sodium lignin sulfonate, the wetting agent LX; Described filler is kaolin, diatomite or potter's clay.
Described compounded pesticides can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art has following advantage and beneficial effect:
(1) this bacterial strain citrus whitefly polypide of being infected from the area, GuangZhou, Guangdong Province separates and obtains, and is China domestic bacterial strain, is not from external introduction, can adapt to the natural environment of this locality.(2) adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.(3) its control efficiency is better than only being used alone the control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, Aschersonia aleyrodis has the very strong insecticidal effect that infects to various pests especially sucking pest.The present invention has good control efficiency to insects such as aleyrodid, aphids for composite dose.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, on basis to a large amount of screenings of bacterial strain, final with the bacterial strain of Aschersonia aleyrodis as biopesticide, and filter out suitable mixed ratio, the embodiment that below provides is a typical scenario of the present invention, and embodiments of the present invention are not limited to this.Following percentage is mass percent.The Aschersonia aleyrodis bacterial strain is AA01-N8, the bacterial strain preserving number: CCTCC No:M-207087, on June 27th, 2007 is in China's typical culture collection center preservation.
Embodiment 1:30% Aschersonia aleyrodis Avermectin suspending agent (diluting 2000 times)
A partly is 60% Aschersonia aleyrodis conidial suspension: with 60% Aschersonia aleyrodis conidial powder, be dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0.In 4～8 ℃ of preservations.
B partly is a wetting powder: 1.8% Avermectin (commercial) 10%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and directly with two parts mixing, promptly obtain 30% Aschersonia aleyrodis Avermectin suspending agent, Aschersonia aleyrodis: the Avermectin mass ratio is 100: 0.3.The virulence test result is as shown in table 1.
Embodiment 2:50% Aschersonia aleyrodis Avermectin finish (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 50%, 1.8% Avermectin 10% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Aschersonia aleyrodis Avermectin finish.Wherein, Aschersonia aleyrodis: the Avermectin mass ratio is 100: 0.36.The virulence test result is as shown in table 1.
Embodiment 3:60% Aschersonia aleyrodis Avermectin finish (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 60%, 1.8% Avermectin 5% (commercial), methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 60% Aschersonia aleyrodis Avermectin finish.Wherein, Aschersonia aleyrodis powder: the mass ratio of Avermectin is 100: 0.15.The virulence test result is as shown in table 1.
Embodiment 4:50% Aschersonia aleyrodis Avermectin wetting powder (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 50%, 1.8% Avermectin 10% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% Aschersonia aleyrodis Avermectin wetting powder.Wherein, Aschersonia aleyrodis powder: the mass ratio of Avermectin is 100: 0.36.The virulence test result is as shown in table 1.
Embodiment 5: compounded pesticides is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun.Its result of the test such as table 1.
Table 1 Aschersonia aleyrodis Avermectin is to the Toxicity Determination (10 of Bemisia tabaci
4Spore/ml, μ g/ml) (behind the medicine 12 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Aschersonia aleyrodis | y＝0.9082x+0.9004 | 0.9321 | 3.27，1.56-6.85 | 100.00 |
| Avermectin (behind the medicine 2 days) | y＝1.5009x+5.1873 | 0.9849 | 0.75，0.46-1.22 | |
| Aschersonia aleyrodis: Avermectin (100: 0.3) | y＝0.5941x+2.4995 | 0.9431 | 1.62，0.35-7.51 | 201.85 |
| Aschersonia aleyrodis: Avermectin (100: 0.36) | y＝0.5363x+2.7878 | 0.9895 | 1.33，0.24-7.26 | 245.86 |
| Aschersonia aleyrodis: Avermectin (100: 0.15) | y＝0.6267x+2.2312 | 0.9863 | 2.62，0.74-9.28 | 124.81 |
| Aschersonia aleyrodis: Avermectin (100: 0.36) | y＝0.5079x+2.9646 | 0.9705 | 1.02，0.15-6.82 | 320.59 |
R
2: determination coefficient; TI: relative virus force index.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (4)
1. the compounded pesticides of an Aschersonia aleyrodis (Aschersonia aleyrodis) and Avermectin is made up of host and assistant agent, it is characterized in that host is 20～60% by mass percentage, and assistant agent is 40～80%; Described host is the mixture of Aschersonia aleyrodis and Avermectin, Aschersonia aleyrodis: the Avermectin mass ratio is 100: 0.15～0.36; Described Aschersonia aleyrodis bacterial strain AA01-N8 preserving number is CCTCC No:M-207087.
2. according to the compounded pesticides of described Aschersonia aleyrodis of claim 1 and Avermectin, it is characterized in that described Aschersonia aleyrodis is Aschersonia aleyrodis conidial powder or Aschersonia aleyrodis spore suspension.
3. according to the compounded pesticides of described Aschersonia aleyrodis of claim 1 and Avermectin, it is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, 20～23% of the stabilizer comprises compounded pesticides, auxiliary agent account for compounded pesticides 7～22%, filler accounts for 5～30% of compounded pesticides; Described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures in Tween-80, methyl green, the sodium lignin sulfonate; Described filler is kaolin, diatomite or potter's clay.
4. according to the compounded pesticides of described Aschersonia aleyrodis of claim 1 and Avermectin, it is characterized in that described compounded pesticides is wetting powder, suspending agent or finish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100292502A CN101317578B (en) | 2008-07-04 | 2008-07-04 | Mixed insecticide of aschersonia aleyrodis webb. and avermectin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100292502A CN101317578B (en) | 2008-07-04 | 2008-07-04 | Mixed insecticide of aschersonia aleyrodis webb. and avermectin |
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| Publication Number | Publication Date |
|---|---|
| CN101317578A CN101317578A (en) | 2008-12-10 |
| CN101317578B true CN101317578B (en) | 2011-01-19 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2008100292502A Expired - Fee Related CN101317578B (en) | 2008-07-04 | 2008-07-04 | Mixed insecticide of aschersonia aleyrodis webb. and avermectin |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102657236B (en) * | 2012-05-26 | 2014-07-09 | 福建农林大学 | Aschersonia oil and compounded insecticide containing same and nitenpyram |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1194780A (en) * | 1997-03-27 | 1998-10-07 | 王玉万 | Plant protection pesticide containing avermectin |
| CN101156601A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of aschersonia aleyrodis webb. and imidacloprid |
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- 2008-07-04 CN CN2008100292502A patent/CN101317578B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1194780A (en) * | 1997-03-27 | 1998-10-07 | 王玉万 | Plant protection pesticide containing avermectin |
| CN101156601A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of aschersonia aleyrodis webb. and imidacloprid |
Non-Patent Citations (1)
| Title |
|---|
| 中华人民共和国农业部.农药室内生物测定试验准则 杀虫剂 第7部分：混配的联合作用测定.中华人民共和国农业行业标准NY/T1154.7-2006.2006,(115472006),1-3. * |
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Granted publication date: 20110119 Termination date: 20160704 |