CN101156601A - Recompounded insecticide of aschersonia aleyrodis webb. and imidacloprid - Google Patents
Recompounded insecticide of aschersonia aleyrodis webb. and imidacloprid Download PDFInfo
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- CN101156601A CN101156601A CNA2007100315181A CN200710031518A CN101156601A CN 101156601 A CN101156601 A CN 101156601A CN A2007100315181 A CNA2007100315181 A CN A2007100315181A CN 200710031518 A CN200710031518 A CN 200710031518A CN 101156601 A CN101156601 A CN 101156601A
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- imidacloprid
- aschersonia aleyrodis
- aleyrodis
- aschersonia
- compounded pesticides
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Abstract
The invention discloses aschersonia aleyrodis and imidacloprid compounded insecticide which is composed of prime solvent and secondary solvent which has the weigh percentages that the prime solvent is 15-50 percent and the secondary solvent is 85-50 percent. The prime solvent is the mixture of aschersonia aleyrodis and imidacloprid, and the weight ratio of the aschersonia aleyrodis and the imidacloprid is 100:1-35. The secondary solvent is stabilizing agent, assistant, and filling. The invention discloses aschersonia aleyrodis and imidacloprid compounded insecticide which can be processed into wettable powder, suspending agent, and water disperser. Long-term infectivity biology researches and an indoor biology measurement prove that the aschersonia aleyrodis has strong infective and insects killing function on multifold pests, in particular pests with chewing mouthparts. The paecilomyces fumosoroseus and imidacloprid compounded insecticide has strong prevention effect on the pests of whitefly, aphids, and so on.
Description
Technical field
The present invention relates to a kind of microorganism insecticide, be meant a kind of environmental protection, pollution-free, the Aschersonia aleyrodis of noresidue and the compounded pesticides of Imidacloprid especially.
Background technology
Aschersonia aleyrodis (Aschersonia aleyrodis Webber) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that Bt is developed immunity to drugs of control, the development and use Aschersonia aleyrodis has important economic implications.Aschersonia aleyrodis is distributed more widely, and sucking mouth partss such as aphid, Bemisia tabaci are had good control efficiency, has a good application prospect.
Imidacloprid (imidacloprid) is widely used efficient, interior suction at present, broad spectrum type nicotinic insecticide, is mainly used in perverse suction formula mouthpart insects such as control aleyrodid, aphid.Along with using historical prolongation, insect has been developed into a major issue to the pesticide resistance of Imidacloprid.Adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, and therefore, the Compositional type insecticide of exploitation Imidacloprid is significant.Still find no at present the relevant Aschersonia aleyrodis and the compounded pesticides of Imidacloprid.
Summary of the invention
In order to overcome the deficiency that above-mentioned prior art exists, the purpose of this invention is to provide a kind of environmental protection, pollution-free, the Aschersonia aleyrodis of noresidue and the compounded pesticides of Imidacloprid.
Purpose of the present invention is achieved through the following technical solutions: the compounded pesticides of a kind of Aschersonia aleyrodis and Imidacloprid, form by host and assistant agent, and be host 15～50% by mass percentage, assistant agent 85～50%; Described host is the mixture of Aschersonia aleyrodis and Imidacloprid, and its mass ratio is: Aschersonia aleyrodis: Imidacloprid=100: 1～35; Described assistant agent is stabilizing agent, auxiliary agent and filler, and described stabilizing agent is one or more mixtures in glycerine, methyl-sulfoxide, the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, sodium lignin sulfonate, saponin or the dispersant dinaphthyl methane-disulfonic acid sodium (NNO); Described filler is talcum powder, potter's clay or clay etc.
Described Aschersonia aleyrodis Aschersonia aleyrodis Webber belongs to Coelomycetes, and seat shell spore belongs to, and is located away from the citrus whitefly polypide of being infected in the area, Guangzhou.The bacterial strain preserving number: CCTCC No:M207087, on June 27th, 2007 is in China's typical culture collection center preservation.Flat cushion of Aschersonia aleyrodis stroma or peltate, little by hair, white is to glassy yellow, diameter 1.0～1.5mm, high 0.4～0.6mm, surrounded by basic unit, basic unit is translucent, white, wide 0.2～0.5mm, forming by close the knitting of mycelia. mycelia is expanded sometimes, and is transparent, and wall is smooth, generous 0.2～6.4 μ m, wall thickness, 0.8～2.2 μ m, pycnidium embeds stroma, and is avette or oblate spheroid is irregular, 120～280 * 150～320 μ m, the aperture of opening wide is arranged, circle, the aperture is covered by orange pionnote.The conidium spindle, crooked sometimes, the top point, monospore, transparent, wall is smooth.The conidiophore list is given birth to or is two verticillate, 2～5 tubular verticils of tool.Verticil is thinner, and is slim and frahile, carefully cylindrical, and do not hold level with both hands and cut, 7.8～17.5 μ m * 1.2～1.8 μ m, lateral filament bears from conidiophore, and is thread, transparent, long 30～100 μ m (rare 350 μ m), wide 1.0～1.6 μ m.
In order to realize the present invention better, the mass ratio of described Aschersonia aleyrodis and Imidacloprid is an Aschersonia aleyrodis: Imidacloprid=100: 3～20.
Described Aschersonia aleyrodis is Aschersonia aleyrodis conidial powder or Aschersonia aleyrodis spore suspension.
Described stabilizing agent, auxiliary agent and filler by mass percentage, be respectively the stabilizer comprises compounded pesticides 5～35%, auxiliary agent account for compounded pesticides 12～20%, filler accounts for 10～35% of compounded pesticides.
The compounded pesticides of described Aschersonia aleyrodis and Imidacloprid can be processed into formulations such as wetting powder, suspending agent or water dispersant.
The present invention compared with prior art has following advantage and beneficial effect: the citrus whitefly polypide that (1) this bacterial strain is infected from the area, GuangZhou, Guangdong Province separates acquisition, is China domestic bacterial strain, is not from external introduction, can adapt to local natural environment.(2) adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.(3) its control efficiency is better than only being used alone the control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, Aschersonia aleyrodis has the very strong insecticidal effect that infects to various pests especially sucking pest.The present invention has good control efficiency to insects such as aleyrodid, aphids for composite dose.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
Following percentage is mass percent, and the Aschersonia aleyrodis bacterial strain is AA01-N8, the bacterial strain preserving number: CCTCC No:M207087, on June 27th, 2007 is in China's typical culture collection center preservation.
Embodiment 1:15% Aschersonia aleyrodis imidacloprid suspending agent (diluting 1000 times)
A partly is 30% Aschersonia aleyrodis conidial suspension: with 30% Aschersonia aleyrodis conidial powder, be dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M pH7.0.In 4～8 ℃ of preservations.
B partly is a wetting powder: 10%WP Imidacloprid (can be directly commercial) 6%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds clay and mends to 100%.
A part and B part respectively account for 50%, and directly with two parts mixing, promptly obtain 15% Aschersonia aleyrodis imidacloprid suspending agent, Aschersonia aleyrodis: the Imidacloprid mass ratio is 100: 20.The virulence test result is as shown in table 1.
Embodiment 2:30% Aschersonia aleyrodis imidacloprid suspending agent (diluting 1000 times)
A partly is 60% Aschersonia aleyrodis conidial suspension: with 60% Aschersonia aleyrodis conidial powder, be dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M.PH7.0 is in 4～8 ℃ of preservations.
B partly is a wetting powder: 10%WP Imidacloprid (commercial) 2%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and directly with two parts mixing, promptly obtain 30% Aschersonia aleyrodis imidacloprid suspending agent, Aschersonia aleyrodis: the Imidacloprid mass ratio is 100: 3.3.The virulence test result is as shown in table 1.
Embodiment 3:50% Aschersonia aleyrodis imidacloprid wettable powder
Aschersonia aleyrodis powder (10
10Individual/g) 50%, 10%WP Imidacloprid 3%, saponin 14%, Tween-80 10% adds potter's clay and mends to 100%, makes 50% Aschersonia aleyrodis imidacloprid wettable powder.Aschersonia aleyrodis: the Imidacloprid mass ratio is 100: 6.The virulence test result is as shown in table 1.
Embodiment 4:50% Aschersonia aleyrodis imidacloprid wettable powder
Aschersonia aleyrodis powder (10
10Individual/g) 50%, 10%WP Imidacloprid 6%, dodecyl sodium sulfate 14%, Tween-80 10% adds clay and mends to 100%, makes 50% Aschersonia aleyrodis imidacloprid wettable powder.Aschersonia aleyrodis: the Imidacloprid mass ratio is 100: 12.The virulence test result is as shown in table 1.
Embodiment 5:15% Aschersonia aleyrodis Imidacloprid water dispersant (diluting 1000 times)
A partly is 30% Aschersonia aleyrodis conidium water dispersant: with 30% Aschersonia aleyrodis conidial powder, be dissolved in the glycerine mixed liquor of the 20% Tween-80 aqueous solution and 10% pH7.0.In 4～8 ℃ of preservations.
B partly is a wetting powder: 10%WP Imidacloprid (commercial) 10%, and sodium lignin sulfonate 6%, dispersant NNO 4% adds clay and mends to 100%.
A part and B part respectively account for 50%, and directly with two parts mixing, promptly obtain 15% Aschersonia aleyrodis Imidacloprid water dispersant, Aschersonia aleyrodis: the Imidacloprid mass ratio is 100: 33.The virulence test result is as shown in table 1.
Embodiment 6: compounded pesticides is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun.Its result of the test such as table 1.
Table 1 Aschersonia aleyrodis Imidacloprid is to the Toxicity Determination (10 of Bemisia tabaci
4Spore/ml, μ g/ml) (behind the medicine 3 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Aschersonia aleyrodis | y＝0.9082x+0.9004 | 0.9321 | 3.27，1.56-6.85 | 100.00 |
| Imidacloprid (behind the medicine 3 days) | y＝1.5799x+3.0199 | 0.9881 | 17.92，16.25-19.76 | |
| Aschersonia aleyrodis: Imidacloprid (100: 20) | y＝0.8090x+1.5604 | 0.9309 | 1.78，0.56-15.73 | 183.71 |
| Aschersonia aleyrodis: Imidacloprid (100: 3.3) | y＝0.8075x+1.7408 | 0.9382 | 1.0864，0.27-4.30 | 300.99 |
| Aschersonia aleyrodis: Imidacloprid (100: 6) | y＝0.7891x+1.8999 | 0.9426 | 0.85，0.19-3.81 | 384.71 |
| Aschersonia aleyrodis: Imidacloprid (100: 12) | y＝0.7980x+1.9074 | 0.9370 | 0.7505，0.16-3.50 | 435.71 |
| Aschersonia aleyrodis: Imidacloprid (100: 33) | y＝0.7319x+1.8726 | 0.9899 | 1.8749，0.52-6.71 | 174.41 |
R
2: determination coefficient; TI: relative virus force index.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (5)
1. the compounded pesticides of Aschersonia aleyrodis and Imidacloprid is made up of host and assistant agent, and it is characterized in that: host is 15～50% by mass percentage, and assistant agent is 85～50%; Described host is the mixture of Aschersonia aleyrodis and Imidacloprid, Aschersonia aleyrodis: the Imidacloprid mass ratio is 100: 1～35; Described assistant agent is stabilizing agent, auxiliary agent and filler, and described stabilizing agent is one or more mixtures in glycerine, methyl-sulfoxide, the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, sodium lignin sulfonate, saponin or the dispersant dinaphthyl methane-disulfonic acid sodium; Described filler is talcum powder, potter's clay or clay; Described Aschersonia aleyrodis bacterial strain preserving number: CCTCC No:M207087, on June 27th, 2007 is in China's typical culture collection center preservation.
2. compounded pesticides according to claim 1 is characterized in that, described Aschersonia aleyrodis: the mass ratio of Imidacloprid is 100: 3～20.
3. compounded pesticides according to claim 1 and 2 is characterized in that, described Aschersonia aleyrodis is Aschersonia aleyrodis conidial powder or Aschersonia aleyrodis spore suspension.
4. compounded pesticides according to claim 1, it is characterized in that, described stabilizing agent, auxiliary agent and filler by mass percentage, be respectively the stabilizer comprises compounded pesticides 5～35%, auxiliary agent account for compounded pesticides 12～20%, filler accounts for 10～35% of compounded pesticides.
5. compounded pesticides according to claim 1 is characterized in that the compounded pesticides of described Aschersonia aleyrodis and Imidacloprid is processed into wetting powder, suspending agent or water dispersant.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2007100315181A CN100531576C (en) | 2007-11-20 | 2007-11-20 | Compounded insecticide of aschersonia aleyrodis webb. and imidacloprid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100315181A CN100531576C (en) | 2007-11-20 | 2007-11-20 | Compounded insecticide of aschersonia aleyrodis webb. and imidacloprid |
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| CN101156601A true CN101156601A (en) | 2008-04-09 |
| CN100531576C CN100531576C (en) | 2009-08-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101317578B (en) * | 2008-07-04 | 2011-01-19 | 华南农业大学 | Mixed insecticide of aschersonia aleyrodis webb. and avermectin |
| CN114460210A (en) * | 2022-01-29 | 2022-05-10 | 国家粮食和物资储备局科学研究院 | Kit and method for detecting various mycotoxins with high precision |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100408672C (en) * | 2005-11-24 | 2008-08-06 | 福建农林大学 | Aschersonia aleyrodis culture medium |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101317578B (en) * | 2008-07-04 | 2011-01-19 | 华南农业大学 | Mixed insecticide of aschersonia aleyrodis webb. and avermectin |
| CN114460210A (en) * | 2022-01-29 | 2022-05-10 | 国家粮食和物资储备局科学研究院 | Kit and method for detecting various mycotoxins with high precision |
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