CN101513193B - Compound desinsection primary agent and pesticide consisting of verticillium lecanii and beta-cypermethrin - Google Patents
Compound desinsection primary agent and pesticide consisting of verticillium lecanii and beta-cypermethrin Download PDFInfo
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- CN101513193B CN101513193B CN2009100377488A CN200910037748A CN101513193B CN 101513193 B CN101513193 B CN 101513193B CN 2009100377488 A CN2009100377488 A CN 2009100377488A CN 200910037748 A CN200910037748 A CN 200910037748A CN 101513193 B CN101513193 B CN 101513193B
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Abstract
The invention discloses a compound desinsection primary agent and pesticide consisting of verticillium lecanii and beta-cypermethrin. The primary agent is a mixture of the verticillium lecanii and the alpha cypermethrin, wherein, the mass ratio between the verticillium lecanii and the alpha cypermethrin is 100:5-50; while the pesticide consists of a primary agent and a secondary agent, according to mass percent, the primary agent accounts for 5-50%, and the secondary agent 50-95%. Through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pests in particular. The compound pesticide consisting of the verticillium lecanii and the beta-cypermethrin of the invention has good effect in preventing and controlling pests such as white fly, plant louse, diamond back moth and the like.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, be meant a kind of environmental protection, pollution-free, the Verticillium lecanii of noresidue and efficient cypermethrin compounded desinsection host and insecticide especially.
Background technology
Verticillium lecanii (Verticillium lecanii (Zimmermann) Viegas) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use Verticillium lecanii has important economic implications.Verticillium lecanii distributes extensively, Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid, thrips etc. is had good control efficiency, has broad application prospects.
Beta-cypermethrin (beta-cypermethrin) is a kind of pyrethroid insecticides, and biologically active is higher, is the efficient isomer of cypermethrin, has and tags and stomach poison function.Insecticidal spectrum is wide, the speed of knocking down is fast, and insecticidal activity is than the cypermethrin height.Be applicable to insect and sanitary insect pest on the various plants such as control cotton, vegetables, fruit tree, tea tree, forest.Be mainly used in insects such as control diamond-back moth, aphid, thrips.
But along with using historical prolongation, insect has been developed into a major issue to the pesticide resistance of beta-cypermethrin.
Adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, and therefore, the Compositional type insecticide of exploitation beta-cypermethrin is significant.Especially utilize the composite living body biological agricultural chemicals of insect pathogenic fungus as beta-cypermethrin, environmentally safe, noresidue have the brand-new mechanism of action that is different from existing chemical insecticide.Still find no at present the relevant Verticillium lecanii and the compounded pesticides of beta-cypermethrin.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the Verticillium lecanii of noresidue and efficient cypermethrin compounded desinsection host are provided.
Another object of the present invention provides the insecticide that is prepared by described desinsection host.
Purpose of the present invention is achieved through the following technical solutions:
A kind of Verticillium lecanii and efficient cypermethrin compounded desinsection host are provided, and described host is the mixture of Verticillium lecanii and beta-cypermethrin, and the mass ratio of described Verticillium lecanii and beta-cypermethrin is 100: 5～50.
In order to realize the present invention better, the mass ratio of described Verticillium lecanii and beta-cypermethrin is preferably 100: 6～and 25.
Described Verticillium lecanii Verticillium lecanii (Zimmermann) Viegas bacterial strain VL-N6, belong to hyphomycetales, Verticillium, be located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou, bacterial strain preserving number: CCTCC No:M-208086, on June 10th, 2008 is in China's typical culture collection center preservation, is to the existing detailed description of Verticillium lecanii in 200810029163.7 the patent application at application number.Described Verticillium lecanii bacteria colony white is to cream-colored, and is thin cotton-shaped, and the back side is colourless to deep mixed yellow.Conidiophore is undeveloped, like nutrient hypha, Dan Sheng is arranged on it, to the bottle stalk of giving birth to or 3～4 verticillate, and the bottle stalk is very thin, and size is because of bacterial strain and cell age is different differs greatly 8.5-40 * 0.8-2.2 μ m; Conidium is oval to cylindrical, two terminal circle, and Dan Sheng is gathered into head at bottle stalk top, and size makes a variation bigger because of bacterial strain reaches, 2.3-10 * 1-2.6 μ m.
As a kind of preferred, described Verticillium lecanii is Verticillium lecanii conidial powder or Verticillium lecanii spore suspension.
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, as preferred scheme, the present invention provides a kind of insecticide that is prepared by described desinsection host simultaneously, described insecticide is made up of host and assistant agent, host is 5～50% by mass percentage, and assistant agent is 50～95%.
Described assistant agent is stabilizing agent, auxiliary agent and filler, and by mass percentage, stabilizer comprises compounded pesticides 5～40%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 10～45%.
One or more mixtures in described stabilizing agent preferably glycerine, paraffin oil, methyl-sulfoxide or the phosphate buffer; One or more mixtures in the preferred Tween-80 of described auxiliary agent, washing powder, methyl green, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium (NNO); Described filler is kaolin, potter's clay or clay.
The compounded pesticides of described Verticillium lecanii and beta-cypermethrin can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art has following advantage and beneficial effect:
(1) the bacterial strain of the present invention Bemisia tabaci polypide of being infected from the area, GuangZhou, Guangdong Province separates and obtains, and is China domestic bacterial strain, is not from external introduction, can adapt to the natural environment of this locality.
(2) the present invention adopts the agricultural chemicals of sub-doses and biopesticide to be mixed, determined to obtain the mixed ratio of good insecticidal effect, the consumption and the persticide residue of beta-cypermethrin have been reduced, adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying pest resistance to insecticide and produce.
(3) control efficiency of compounded pesticides of the present invention is better than only using a kind of control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, Verticillium lecanii has the very strong insecticidal effect that infects to various pests especially sucking pest.The compounded pesticides of Verticillium lecanii of the present invention and beta-cypermethrin has good control efficiency to insects such as aleyrodid, aphid, diamond-back moths.
Embodiment
What insecticide of the present invention played insecticidal action is described host, being equipped with the effect that assistant agent obtains conveniently to use according to the existing routine techniques in this area gets final product, the present invention is described in further detail below in conjunction with preferred embodiment, but therefore do not limit scope of the present invention.
Be mass percent described in following examples, described Verticillium lecanii is the VL-N6 bacterial strain, the bacterial strain preserving number: CCTCC No:M-208086, preservation date are on June 10th, 2008, specifically describe to see that application number is in 200810029163.7 the patent application.
Embodiment 1:20% Verticillium lecanii beta-cypermethrin finish (dilution is 1000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 20%, beta-cypermethrin 3%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 50% Verticillium lecanii beta-cypermethrin finish.Wherein, Verticillium lecanii bacterium powder: the mass ratio of beta-cypermethrin is 100: 15.The virulence test result is as shown in table 1.
Embodiment 2:5% Verticillium lecanii beta-cypermethrin suspending agent (dilution is 1000 times during use)
A partly is 10% Verticillium lecanii conidial suspension: 10% Verticillium lecanii conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, transfers pH7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: beta-cypermethrin 5.0%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds clay and mends to 100%.
A part and B part respectively account for 50%, directly two parts mixing are promptly obtained 5% Verticillium lecanii beta-cypermethrin suspending agent, and described Verticillium lecanii: the beta-cypermethrin mass ratio is 100: 50.The virulence test result is as shown in table 1.
Embodiment 3:50% Verticillium lecanii beta-cypermethrin wetting powder (dilution is 1000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, beta-cypermethrin 3%, saponin 14%, Tween-80 10% adds potter's clay and mends to 100%, makes 50% Paecilomyces varioti beta-cypermethrin wetting powder.Wherein, Verticillium lecanii bacterium powder: the beta-cypermethrin mass ratio is 100: 6.The virulence test result is as shown in table 1.
Embodiment 4:50% Verticillium lecanii beta-cypermethrin finish (dilution is 1000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 50%, beta-cypermethrin 2.5%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin is mended to 100%, makes 50% Verticillium lecanii beta-cypermethrin finish.Wherein, Verticillium lecanii bacterium powder: the mass ratio of beta-cypermethrin is 100: 5.The virulence test result is as shown in table 1.
Embodiment 5:20% Verticillium lecanii beta-cypermethrin finish (dilution is 1000 times during use)
Verticillium lecanii bacterium powder (10
10Individual/g) 20%, beta-cypermethrin 5.0%, methyl green 2%, Tween-80 5%, paraffin oil 23%, kaolin are mended to 100%, and mixing promptly obtains 10% Verticillium lecanii beta-cypermethrin finish, Verticillium lecanii: the beta-cypermethrin mass ratio is 100: 25.The virulence test result is as shown in table 1.
Embodiment 6: mixture is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 Verticillium lecanii beta-cypermethrin is to the Toxicity Determination of Bemisia tabaci
(10
4Spore/ml, μ g/ml) (behind the medicine 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Verticillium lecanii | y＝0.3744x+3.3240 | 0.9814 | 2.99，0.98-9.16 | 100.00 |
| Beta-cypermethrin (behind the medicine 3 days) | y＝1.5799x+3.0199 | 0.9881 | 17.92，16.25-19.76 | |
| Example 1: Verticillium lecanii: beta-cypermethrin (100: 15) | y＝0.3575x+3.4837 | 0.9874 | 1.74，0.48-62.66 | 171.84 |
| Example 2: Verticillium lecanii: beta-cypermethrin (100: 50) | y＝0.3819x+3.3316 | 0.9878 | 2.33，0.74-73.94 | 128.33 |
| Example 3: Verticillium lecanii: beta-cypermethrin (100: 6) | y＝0.3709x+3.4880 | 0.9851 | 1.19，0.31-45.13 | 251.26 |
| Example 4: Verticillium lecanii: beta-cypermethrin (100: 5) | y＝0.3475x+3.6088 | 0.9768 | 1.01，0.23-42.60 | 296.04 |
| Example 5: Verticillium lecanii: beta-cypermethrin (100: 25) | y＝0.3892x+3.3599 | 0.9811 | 1.64，0.49-55.20 | 182.32 |
R
2: determination coefficient; TI: relative virus force index.
Claims (7)
1. a Verticillium lecanii (Verticillium lecanii (Zimmermann) Viegas) and efficient cypermethrin compounded desinsection host, it is characterized in that described host is the mixture of Verticillium lecanii and beta-cypermethrin, the mass ratio of described Verticillium lecanii and beta-cypermethrin is 100: 5～50; The bacterial strain preserving number of described verticillium lecanii strain VL-N6 is CCTCCNo:M-208086.
2. according to described Verticillium lecanii of claim 1 and efficient cypermethrin compounded desinsection host, it is characterized in that described Verticillium lecanii and beta-cypermethrin mass ratio are 100: 6～25.
3. according to described Verticillium lecanii of claim 1 and efficient cypermethrin compounded desinsection host, it is characterized in that described Verticillium lecanii is Verticillium lecanii bacterium powder or Verticillium lecanii spore suspension.
4. an insecticide that is prepared by the described desinsection host of claim 1 is characterized in that being made up of host and assistant agent, and host is 5～50% by mass percentage, and assistant agent is 50～95%.
5. according to the described insecticide of claim 4, it is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, stabilizer comprises compounded pesticides 5～40%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 10～45%.
6. according to the described insecticide of claim 5, it is characterized in that described stabilizing agent is one or more mixtures in glycerine, paraffin oil, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures that select in Tween-80, washing powder, methyl green, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium; Described filler is kaolin, potter's clay or clay.
7. according to claim 4,5 or 6 described insecticides, it is characterized in that described insecticide is wetting powder, suspending agent or finish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100377488A CN101513193B (en) | 2009-03-10 | 2009-03-10 | Compound desinsection primary agent and pesticide consisting of verticillium lecanii and beta-cypermethrin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100377488A CN101513193B (en) | 2009-03-10 | 2009-03-10 | Compound desinsection primary agent and pesticide consisting of verticillium lecanii and beta-cypermethrin |
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| CN101513193A CN101513193A (en) | 2009-08-26 |
| CN101513193B true CN101513193B (en) | 2011-09-14 |
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| CN102613154A (en) * | 2012-03-27 | 2012-08-01 | 泰山医学院 | Method for controlling flies by utilizing two kinds of poison fly ropes |
| CN102630659A (en) * | 2012-03-27 | 2012-08-15 | 泰山医学院 | Double-effect fly poisoning rope for flies and manufacturing method of double-effect fly poisoning rope |
| CN106417289A (en) * | 2016-08-27 | 2017-02-22 | 梁小萍 | Pesticide for preventing and controlling litchi whiteflies and preparation method thereof |
| CN106577776A (en) * | 2016-11-25 | 2017-04-26 | 河池市农业科学研究所 | Microbial pesticide for treating corn diseases |
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Non-Patent Citations (2)
| Title |
|---|
| 《6种常用农药与球孢白僵菌和蜡蚧轮枝菌的相容性》;谷祖敏等;《农药》;20060531;第45卷(第5期);325-326 * |
| 谷祖敏等.《6种常用农药与球孢白僵菌和蜡蚧轮枝菌的相容性》.《农药》.2006,第45卷(第5期),325-326. * |
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