CN101317579B - Mixed insecticide of aschersonia aleyrodis webb. and high-efficiency cypermethrin - Google Patents
Mixed insecticide of aschersonia aleyrodis webb. and high-efficiency cypermethrin Download PDFInfo
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- CN101317579B CN101317579B CN2008100292517A CN200810029251A CN101317579B CN 101317579 B CN101317579 B CN 101317579B CN 2008100292517 A CN2008100292517 A CN 2008100292517A CN 200810029251 A CN200810029251 A CN 200810029251A CN 101317579 B CN101317579 B CN 101317579B
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- cypermethrin
- aschersonia aleyrodis
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Abstract
The invention discloses an insecticide compounded by aschersonia aleyrodis and Alpha-cypermethrin, which consists of a main agent and an auxiliary agent. According to the mass percentage, the main agent occupies 32-61% and the auxiliary agent occupies 39-68%; the main agent is the mixture of the aschersonia aleyrodis and the Alpha-cypermethrin with the mass proportion of 100:0.1-0.6; the auxiliary agent is stabilizer, assisting agent and filling material. The compounded insecticide of the invention can be processed into wettable powder, suspension agent or oil agent; according to long-term infection biological study and indoor biological testing, the aschersonia aleyrodis has extremely strong infection insecticidal effects on a plurality of pests, especially for the pests of piercing-sucking mouthpart. The compounded agent of the invention has excellent control effects on the pests such as whitefly, aphid, etc.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of biological insecticides, be meant a kind of environmental protection, pollution-free, the Aschersonia aleyrodis of noresidue and the compounded pesticides of beta-cypermethrin especially.
Background technology
Aschersonia aleyrodis (Aschersonia aleyrodis Webber) is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that Bt is developed immunity to drugs of control, Aschersonia aleyrodis is distributed more widely, suckings pest such as aphid, Bemisia tabaci are had good control efficiency, and the development and use Aschersonia aleyrodis has important economic implications and wide application prospect.
Beta-cypermethrin (beta-cypermethrin) is a pyrethroid insecticides, and biologically active is higher, is the efficient isomer of cypermethrin, has and tags and stomach poison function, and insecticidal spectrum is wide, the speed of knocking down is fast, and insecticidal activity is than the cypermethrin height.Be applicable to pest and sanitary insect pest on the various plants such as control cotton, vegetables, fruit tree, tea tree, forest.Be mainly used in insects such as control aleyrodid, aphid, thrips, but along with beta-cypermethrin uses historical prolongation, insect to the pesticide resistance of beta-cypermethrin developed into demand side to the major issue that solves.It is an important method that improves the quick-acting of microorganism insecticide and reduce residue of pesticide that the chemical pesticide of employing sub-doses mixes with biopesticide, therefore, the Compositional type insecticide of exploitation beta-cypermethrin, and seek effective mixed ratio, will have great importance.Still find no at present the relevant Aschersonia aleyrodis and the compounded pesticides of beta-cypermethrin.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the Aschersonia aleyrodis of noresidue and the compounded pesticides of beta-cypermethrin are provided.
Purpose of the present invention is achieved through the following technical solutions: Aschersonia aleyrodis that is provided and efficient cypermethrin compounded insecticide are made up of host and assistant agent, and host is 32～61% by mass percentage, and assistant agent is 39～68%; Described host is the mixture of Aschersonia aleyrodis and beta-cypermethrin, Aschersonia aleyrodis: the beta-cypermethrin mass ratio is 100: 0.1～0.6.
In order to realize the present invention better, the mass ratio of described Aschersonia aleyrodis and beta-cypermethrin is an Aschersonia aleyrodis: beta-cypermethrin=100: 0.18～0.6.
Described Aschersonia aleyrodis is Aschersonia aleyrodis conidial powder or Aschersonia aleyrodis spore suspension.
Described Aschersonia aleyrodis (Aschersonia aleyrodis Webber) belongs to Coelomycetes, and seat shell spore belongs to, and is located away from the citrus whitefly polypide of being infected in the area, Guangzhou.Strains A A01-N8 preserving number: CCTCC No:M-207087, on June 27th, 2007 disclosed in the patent application of number of patent application 200710031516.2 in China's typical culture collection center preservation.Flat cushion of Aschersonia aleyrodis stroma or peltate, little by hair, white is to glassy yellow, diameter 1.0～1.5mm, high 0.4～0.6mm, surrounded by basic unit, basic unit is translucent, white, wide 0.2～0.5mm is formed by close the knitting of mycelia. and mycelia is expanded sometimes, and is transparent, wall is smooth, generous 0.2～6.4 μ m, wall thickness 0.8～2.2 μ m, pycnidium embeds stroma, and is avette or oblate spheroid is irregular, 120～280 * 150～320 μ m, the aperture of opening wide is arranged, circle, the aperture is covered by orange pionnote.The conidium spindle, crooked sometimes, the top point, monospore, transparent, wall is smooth.The conidiophore list is given birth to or is two verticillate, 2～5 tubular verticils of tool.Verticil is thinner, and is slim and frahile, carefully cylindrical, do not hold level with both hands and cuts 7.8～17.5 μ m * 1.2～1.8 μ m, and lateral filament bears from conidiophore, and is thread, transparent, long 30～100 μ m (rare 350 μ m), wide 1.0～1.6 μ m.
Described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, 20～23% of the stabilizer comprises compounded pesticides, auxiliary agent account for compounded pesticides 7～22%, filler accounts for 8～31% of compounded pesticides.
Described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures among Tween-80, methyl green, sodium lignin sulfonate, the wetting agent LX; Described filler is kaolin, diatomite or potter's clay.
The compounded pesticides of described Aschersonia aleyrodis and beta-cypermethrin can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art has following advantage and beneficial effect:
(1) bacterial strain that uses of the compounded pesticides of the present invention citrus whitefly polypide of being infected from the area, GuangZhou, Guangdong Province separates and obtains, and is China domestic bacterial strain, is not from external introduction, can adapt to the natural environment of this locality.
(2) compounded pesticides of the present invention has adapted to the requirement that organic food is produced, and environmentally safe, noresidue help delaying pest resistance to insecticide and produce.
(3) compounded pesticides control efficiency of the present invention is better than only being used alone the control efficiency of medicament.Through long-term biological study and the indoor bioassay of infecting, Aschersonia aleyrodis has the very strong insecticidal effect that infects to various pests especially sucking pest.The present invention has good control efficiency to insects such as aleyrodid, aphids for composite dose.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, on basis to a large amount of screenings of bacterial strain, final with the bacterial strain of Aschersonia aleyrodis as biopesticide, and filter out suitable mixed ratio, the embodiment that below provides is a typical scenario of the present invention, and embodiments of the present invention are not limited to this.Following percentage is mass percent.Strains A A01-N8 preserving number: CCTCC No:M-207087, on June 27th, 2007 is in China's typical culture collection center preservation.
Embodiment 1:30% Aschersonia aleyrodis beta-cypermethrin suspending agent (diluting 2000 times)
A partly is 60% Aschersonia aleyrodis conidial suspension: with 60% Aschersonia aleyrodis conidial powder, be dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 4.5%EC beta-cypermethrin (commercial) 8%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and directly with two parts mixing, promptly obtain 30% Aschersonia aleyrodis beta-cypermethrin suspending agent, Aschersonia aleyrodis: the beta-cypermethrin mass ratio is 100: 0.6.The virulence test result is as shown in table 1.
Embodiment 2:50% Aschersonia aleyrodis beta-cypermethrin finish (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 50%, 4.5%EC beta-cypermethrin 4% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Aschersonia aleyrodis beta-cypermethrin finish.Wherein, Aschersonia aleyrodis: the beta-cypermethrin mass ratio is 100: 0.36.The virulence test result is as shown in table 1.
Embodiment 3:60% Aschersonia aleyrodis beta-cypermethrin wetting powder (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 60%, 4.5%EC beta-cypermethrin 4% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 3%, wetting agent LX 2%, kaolin is mended to 100%, makes 60% Aschersonia aleyrodis beta-cypermethrin wetting powder.Wherein, Aschersonia aleyrodis powder: the mass ratio of beta-cypermethrin is 100: 0.3.The virulence test result is as shown in table 1.
Embodiment 4:50% Aschersonia aleyrodis beta-cypermethrin wetting powder (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 50%, 4.5%EC beta-cypermethrin 2% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% Aschersonia aleyrodis beta-cypermethrin wetting powder.Wherein, Aschersonia aleyrodis powder: the mass ratio of beta-cypermethrin is 100: 0.18.The virulence test result is as shown in table 1.
Embodiment 5:50% Aschersonia aleyrodis beta-cypermethrin wetting powder (diluting 2000 times)
Aschersonia aleyrodis powder (10
10Individual/g) 50%, 4.5%EC beta-cypermethrin 1.11% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% Aschersonia aleyrodis beta-cypermethrin wetting powder.Wherein, Aschersonia aleyrodis powder: the mass ratio of beta-cypermethrin is 100: 0.1.
Embodiment 6: compounded pesticides is to the Toxicity Determination of Bemisia tabaci (Bemisia tabaci)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access Bemisia tabaci adults, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, treat that Bemisia tabaci on the cucumber leaves grows to 2 ages during nymph, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun.Its result of the test sees Table 1.
Table 1 Aschersonia aleyrodis beta-cypermethrin is to the Toxicity Determination (10 of Bemisia tabaci
4Spore/ml, μ g/ml) (behind the medicine 12 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Aschersonia aleyrodis | y＝0.9082x+ 0.9004 | 0.9321 | 3.27，1.56-6.85 | 100.00 |
| Beta-cypermethrin (behind the medicine 3 days) | y＝1.5799x +3.0199 | 0.9881 | 17.92，16.25-19.76 | |
| Aschersonia aleyrodis: beta-cypermethrin (100: 0.6) | y＝0.5378x+ 2.6440 | 0.9313 | 2.40，0.55-10.23 | 136.25 |
| Aschersonia aleyrodis: beta-cypermethrin (100: 0.36) | y＝0.4213x+ 3.3098 | 0.9305 | 1.33，0.12-8.91 | 245.86 |
| Aschersonia aleyrodis: beta-cypermethrin (100: 0.3) | y＝0.5113x+ 2.8505 | 0.9767 | 1.60，0.29-8.58 | 204.36 |
| Aschersonia aleyrodis: beta-cypermethrin (100: 0.18) | y＝0.5235x+ 2.7749 | 0.9396 | 1.78，0.36-8.82 | 183.70 |
R
2: determination coefficient; TI: relative virus force index.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (6)
1. the compounded pesticides of an Aschersonia aleyrodis (Aschersonia aleyrodis Webber) and beta-cypermethrin is made up of host and assistant agent, it is characterized in that host is 32～61% by mass percentage, and assistant agent is 39～68%; Described host is the mixture of Aschersonia aleyrodis and beta-cypermethrin, Aschersonia aleyrodis: the beta-cypermethrin mass ratio is 100: 0.1～0.6; Described Aschersonia aleyrodis bacterial strain on June 27th, 2007 in China's typical culture collection center preservation, preserving number is CCTCC No:M-207087.
2. according to the compounded pesticides of described Aschersonia aleyrodis of claim 1 and beta-cypermethrin, it is characterized in that described Aschersonia aleyrodis: the mass ratio of beta-cypermethrin is 100: 0.18～0.6.
3. according to the compounded pesticides of claim 1 or 2 described Aschersonia aleyrodis and beta-cypermethrin, it is characterized in that described Aschersonia aleyrodis is Aschersonia aleyrodis conidial powder or Aschersonia aleyrodis spore suspension.
4. according to the compounded pesticides of described Aschersonia aleyrodis of claim 1 and beta-cypermethrin, it is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, 20～23% of the stabilizer comprises compounded pesticides, auxiliary agent account for compounded pesticides 7～22%, filler accounts for 8～31% of compounded pesticides.
5. according to the compounded pesticides of described Aschersonia aleyrodis of claim 4 and beta-cypermethrin, it is characterized in that described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures in Tween-80, methyl green, the sodium lignin sulfonate; Described filler is kaolin, diatomite or potter's clay.
6. according to the compounded pesticides of described Aschersonia aleyrodis of claim 1 and beta-cypermethrin, it is characterized in that described compounded pesticides is wetting powder, suspending agent or finish.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100292517A CN101317579B (en) | 2008-07-04 | 2008-07-04 | Mixed insecticide of aschersonia aleyrodis webb. and high-efficiency cypermethrin |
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| CN2008100292517A CN101317579B (en) | 2008-07-04 | 2008-07-04 | Mixed insecticide of aschersonia aleyrodis webb. and high-efficiency cypermethrin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101182467A (en) * | 2007-11-20 | 2008-05-21 | 华南农业大学 | Aschersonia aleyrodis strain and uses thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182467A (en) * | 2007-11-20 | 2008-05-21 | 华南农业大学 | Aschersonia aleyrodis strain and uses thereof |
Non-Patent Citations (1)
| Title |
|---|
| 邱宝利，任顺祥等.国内烟粉虱B生物型的分布及其控制措施研究.《华东昆虫学报》.2003,第12卷(第2期),第27-31页. * |
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