CN101331884B - Paecilomyces javanicus and acetamiprid compounded pesticides - Google Patents
Paecilomyces javanicus and acetamiprid compounded pesticides Download PDFInfo
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- CN101331884B CN101331884B CN2008100297436A CN200810029743A CN101331884B CN 101331884 B CN101331884 B CN 101331884B CN 2008100297436 A CN2008100297436 A CN 2008100297436A CN 200810029743 A CN200810029743 A CN 200810029743A CN 101331884 B CN101331884 B CN 101331884B
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- acetamiprid
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Abstract
The invention discloses a mixed insecticide of paecilomyces javanicus and acetamiprid, which consists of a main agent and an adjuvant; according to a weight percent, the main agent is 22 - 61 percent, and the adjuvant is 39 - 78 percent; the main agent is the mixture of the paecilomyces javanicus and the acetamiprid, and the weight ratio of the paecilomyces javanicus and the acetamiprid is 100:1.5 - 10; and the adjuvant is a stabilizer, a promoter and a filler. The mixed insecticide of the invention can be processed into a wettable powder, a suspending agent or an oil agent; and by long-term infection biology researches and indoor creature tests, the mixed insecticide has strong infection insecticidal effect on a plurality of pests, in particular to the pests with piercing-sucking mouthparts, and has good preventing effect on such pests as diamondback moth, aphid, thrips, etc.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of microorganism insecticide, be meant a kind of environmental protection, pollution-free, the Paecilomyces javanicus of noresidue and the compounded pesticides of Acetamiprid especially.
Background technology
Paecilomyces javanicus Isaria javanicus (Friendrichs ﹠amp; Bally) be a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, have characteristics such as the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use Paecilomyces javanicus has important economic implications.Paecilomyces javanicus distributes extensively, red fire ant, prodenia litura, aleyrodid and diamond-back moth etc. is had good control efficiency, has broad application prospects.
The novel nicotinamide insecticide of insecticidal actions such as Acetamiprid (acetamiprid) is that having of using at present tagged, stomach toxicity, infiltration and interior suction, act on the nAChR of insect nervous system postsynaptic membrane and nerve on every side thereof, make insect keep excited, paralysis death then.Acetamiprid is mainly used in the insect of sucking mouth partss such as the homoptera pest of control such as leafhopper, aleyrodid, aphid, lepidoptera pest such as diamond-back moth, peach fruit borer etc., coleopteran pest such as longicorn, thrips insect such as thrips etc.; Have and good kill ovum, larvicidal activity, but it is not high that non-target pest is shown as activity, it is certain residual to use the back can detect on fruits and vegetables simultaneously.
Adopting the agriculture chemical compounding technology or mixing application technique is that to overcome insecticidal spectrum narrow or delay an important method of pest resistance to insecticide, it is to improve the quick-acting of microorganism insecticide and reduce a residual important method of chemical pesticide that the chemical pesticide of employing sub-doses mixes with biopesticide, the mixed ratio of screening Paecilomyces javanicus and Acetamiprid the best will have great importance.Still find no at present the relevant Paecilomyces javanicus and the compounded pesticides of Acetamiprid.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the Paecilomyces javanicus of noresidue and the compounded pesticides of Acetamiprid are provided.
Purpose of the present invention realizes by following technical proposals:
The compounded pesticides of a kind of Paecilomyces javanicus and Acetamiprid is provided, is made up of host and assistant agent, host is 22～61% by mass percentage, and assistant agent is 39～78%; Described host is the mixture of Paecilomyces javanicus and Acetamiprid, described Paecilomyces javanicus: the Acetamiprid mass ratio is 100: 1.5～10.
Described Paecilomyces javanicus Isaria javanicus (Friendrichs ﹠amp; Bally) bacterial strain IJ-N2; belong to hyphomycetales, paecilomyces is done the nature reserve, hilllock from Guangxi province and is separated acquisition by the diamond-back moth polypide of entomogenous fungi natural infection; the bacterial strain preserving number: CCTCC No:M208085, on June 10th, 2007 is in China's typical culture collection center preservation.In being 200810029158.6 patent application, application number has a detailed description.Paecilomyces javanicus bacterium colony on the Cha Shi medium is stratiform, and one deck edge of median rise is irregular, khaki; bacterium colony back side central authorities white, outer rim is faint yellow, and column or ellipse expand in bottle metulae portion; upwards attenuate suddenly, 7.5-12.5 * 2-2.5 μ m, 2-3 wheel is born on the conidiophore.Conidium cylindricality or oblong, 4-6.7 * 1-1.5 μ m is discrete long-chain.
In order to realize the present invention better, the mass ratio of described Paecilomyces javanicus and Acetamiprid is a Paecilomyces javanicus: Acetamiprid=100: 2.5～6.
As a kind of preferred, described Paecilomyces javanicus is conidial powder or spore suspension.
Described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, stabilizer comprises compounded pesticides 23～30%, auxiliary agent account for compounded pesticides 7～22%, filler accounts for compounded pesticides 18～33%.
Described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures among Tween-80, methyl green, sodium lignin sulfonate, the wetting agent LX; Described filler is kaolin, diatomite or potter's clay.
The compounded pesticides of described Paecilomyces javanicus and Acetamiprid can be processed into formulations such as wetting powder, suspending agent or finish.
The present invention compared with prior art; have following advantage and beneficial effect: (1) this bacterial strain is done the nature reserve, hilllock from Guangxi province and is separated acquisition by the diamond-back moth polypide of entomogenous fungi natural infection; being China domestic bacterial strain, is not from external introduction, can adapt to local natural environment.(2) adapted to the requirement that organic food is produced, environmentally safe, noresidue help delaying the generation of pest resistance to insecticide.(3) its control efficiency is better than only using a kind of control efficiency of medicament.Through infecting biological study and indoor bioassay, the compounded pesticides of Paecilomyces javanicus and Acetamiprid has good control efficiency to insects such as prodenia litura, aleyrodid and diamond-back moths.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, following embodiment is a preferred implementation of the present invention, but spiritual essence of the present invention and principle are to be based upon great deal of experiment data just to be summed up, simple the substituting of the variation of simple proportioning ratio, similar material can be realized according to this area routine techniques, do not give unnecessary details in an embodiment one by one, but therefore do not limit the present invention.
Following percentage is mass percent, and fungi Imperficti strain is IJ-N2, the bacterial strain preserving number: CCTCC No:M208085, on June 10th, 2007 is in China's typical culture collection center preservation.
Embodiment 1:20% Paecilomyces javanicus acetamiprid suspending agent (diluting 2000 times)
A partly is 40% Paecilomyces javanicus conidial suspension: 40% Paecilomyces javanicus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, and pH7.0 is in 4～8 ℃ of preservations.
B partly is a wetting powder: Acetamiprid (commercial) 4%, and Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 20% Paecilomyces varioti acetamiprid suspending agent, Paecilomyces javanicus: the Acetamiprid mass ratio is 100: 10.The virulence test result is as shown in table 1.
Embodiment 2:50% Paecilomyces javanicus Acetamiprid finish (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 50%, Acetamiprid 2% (commercial), Tween-80 5%, paraffin oil 23%, methyl green 2% adds diatomite and mends to 100%, makes 50% Paecilomyces varioti Acetamiprid finish.Wherein, Paecilomyces javanicus bacterium powder: the Acetamiprid mass ratio is 100: 4.The virulence test result is as shown in table 1.
Embodiment 3:60% Paecilomyces javanicus Acetamiprid wetting powder (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 60%, Acetamiprid 1% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 3%, wetting agent LX 2%, kaolin is mended to 100%, makes 60% Paecilomyces javanicus Acetamiprid wetting powder.Wherein, Paecilomyces javanicus bacterium powder: the mass ratio of Acetamiprid is 100: 1.67.The virulence test result is as shown in table 1.
Embodiment 4:50% Paecilomyces javanicus Acetamiprid wetting powder (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 50%, Acetamiprid 5% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, makes 50% Paecilomyces javanicus Acetamiprid wetting powder.Wherein, Paecilomyces javanicus bacterium powder: the mass ratio of Acetamiprid is 100: 6.The virulence test result is as shown in table 1.
Embodiment 5:60% Paecilomyces javanicus Acetamiprid wetting powder (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 60%, Acetamiprid 0.9% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 3%, wetting agent LX 2%, kaolin is mended to 100%, makes 60% Paecilomyces javanicus Acetamiprid wetting powder.Wherein, Paecilomyces javanicus bacterium powder: the mass ratio of Acetamiprid is 100: 2.5.The virulence test result is as shown in table 1.
Embodiment 6:60% Paecilomyces javanicus Acetamiprid wetting powder (diluting 2000 times)
Paecilomyces javanicus bacterium powder (10
10Individual/g) 60%, Acetamiprid 1.5% (commercial), methyl green 2%, Tween-80 10%, sodium lignin sulfonate 3%, wetting agent LX 2%, kaolin is mended to 100%, makes 60% Paecilomyces javanicus Acetamiprid wetting powder.Wherein, Paecilomyces javanicus bacterium powder: the mass ratio of Acetamiprid is 100: 1.5.
Embodiment 7: mixture is to the Toxicity Determination of diamond-back moth (Plutella xyostella)
During Toxicity Determination, (Brassica campestris L.) makes test plant with cabbage mustard, potted plant seedling is put into dependent insect cage, and access diamond-back moth adult, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, when treating that diamond-back moth on the cabbage mustard blade grows to 2 ages, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 Paecilomyces javanicus Acetamiprid is to the Toxicity Determination of diamond-back moth
(10
3Spore/ml, μ g/ml) (behind the medicine 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Paecilomyces javanicus | y＝0.5920x+ 2.1039 | 0.9744 | 7.80，3.04-19.95 | 100.00 |
| Acetamiprid (behind the medicine 3 days) | y＝1.6004x +3.3185 | 0.9700 | 11.92，9.14-13.81 | |
| Example 1: Paecilomyces javanicus: Acetamiprid (100: 10) | y＝0.6930x+ 1.6445 | 0.9522 | 6.95，2.93-16.60 | 112.23 |
| Example 2: Paecilomyces javanicus: Acetamiprid (100: 4) | y＝0.6275x+ 2.0171 | 0.9824 | 5.67，2.11-15.14 | 137.57 |
| Example 3: Paecilomyces javanicus: Acetamiprid (100: 1.67) | y＝0.6214x+ 2.0622 | 0.9736 | 5.34，1.94-14.79 | 146.07 |
| Example 4: Paecilomyces javanicus: Acetamiprid (100: 6) | y＝0.5862x+ 2.2422 | 0.9844 | 5.06，1.72-14.79 | 154.15 |
| Example 5: Paecilomyces javanicus: Acetamiprid (100: 2.5) | y＝0.5727x+ 2.2973 | 0.9957 | 5.23，1.78-15.49 | 149.13 |
R
2: determination coefficient; TI: relative virus force index.
Claims (4)
1. the compounded pesticides of a Paecilomyces javanicus (Isaria javanicus) and Acetamiprid is made up of host and assistant agent, it is characterized in that host is 22～61% by mass percentage, and assistant agent is 39～78%; Described host is the mixture of Paecilomyces javanicus and Acetamiprid, described Paecilomyces javanicus: the Acetamiprid mass ratio is 100: 2.5～6; Described fungi Imperficti strain IJ-N2, preserving number are CCTCC No:M208085.
2. compounded pesticides according to claim 1 is characterized in that described Paecilomyces javanicus is conidial powder or spore suspension.
3. compounded pesticides according to claim 1 is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, stabilizer comprises compounded pesticides 23～30%, auxiliary agent account for compounded pesticides 7～22%, surplus is a filler; Described stabilizing agent is phosphate buffer or the paraffin oil of glycerine, 0.21M; Described auxiliary agent is one or more mixtures in Tween-80, methyl green, the sodium lignin sulfonate; Described filler is kaolin, diatomite or potter's clay.
4. compounded pesticides according to claim 1 is characterized in that described compounded pesticides is wetting powder, suspending agent or finish.
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| CN2008100297436A CN101331884B (en) | 2008-07-25 | 2008-07-25 | Paecilomyces javanicus and acetamiprid compounded pesticides |
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| CN2008100297436A CN101331884B (en) | 2008-07-25 | 2008-07-25 | Paecilomyces javanicus and acetamiprid compounded pesticides |
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| CN101331884A CN101331884A (en) | 2008-12-31 |
| CN101331884B true CN101331884B (en) | 2011-01-19 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1299593A (en) * | 2001-01-05 | 2001-06-20 | 深圳市诺普信农化有限公司 | Abmectin-acetamiprid pesticide composition |
| CN1389113A (en) * | 2002-07-22 | 2003-01-08 | 张淑静 | Acethion pesticide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1299593A (en) * | 2001-01-05 | 2001-06-20 | 深圳市诺普信农化有限公司 | Abmectin-acetamiprid pesticide composition |
| CN1389113A (en) * | 2002-07-22 | 2003-01-08 | 张淑静 | Acethion pesticide |
Non-Patent Citations (1)
| Title |
|---|
| 中华人民共和国农业部.农药室内生物测定试验准则 杀虫剂 第7部分：混配的联合作用测定.中华人民共和国农业行业标准NY/T 1154.7-2006.2006,全文. * |
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Effective date of registration: 20150119 Address after: 529500, Vientiane Industrial Park, North customs town, Yangdong County, Yangjiang City, Guangdong Province, No. seven, 1, Chicheng Road Patentee after: GUANGDONG XINJINGXIANG BIOENGINEERING Co.,Ltd. Address before: 510642 Tianhe District, Guangdong, No. five road, No. 483, Patentee before: South China Agricultural University |
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