CN101328462B - Microspore strain and use thereof - Google Patents
Microspore strain and use thereof Download PDFInfo
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- CN101328462B CN101328462B CN2008101156045A CN200810115604A CN101328462B CN 101328462 B CN101328462 B CN 101328462B CN 2008101156045 A CN2008101156045 A CN 2008101156045A CN 200810115604 A CN200810115604 A CN 200810115604A CN 101328462 B CN101328462 B CN 101328462B
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Abstract
The invention provides a microspore strain extracted from paranosema locustae and an application thereof. The strain has quite strong specificity and has specific action of killing grassland locusts and farmland migratory locusts, and the killing effect is relatively good. The microspore strain extracted from the paranosema locustae of the invention can be used to control tens of locusts such as farmland migratory locusts, grassland locusts, paranosema locustae, bamboo locusts and rice locusts, etc.
Description
Technical field
The present invention relates to little spore product scope, be specifically related to little spore of separating in a kind of car locust body with and uses thereof.
Background technology
The plague of locusts is global natural disaster, and agriculture production has been caused very big influence.For many years, the plague of locusts once took place large-arealy frequently to break out, and comprised the farmland migratory locusts, grassland grasshopper, the cotton locust of oryza chinensis and forestry, bamboo locust.Only farmland migratory locusts and grassland grasshopper take place respectively 2,000 ten thousand mu times and 6,000 ten thousand mu times every year, have had a strong impact on husbandry production.The U.S. adopts two-striped grasshopper and black locust to cultivate beyond the nosema locustae as propagation host from the eighties in 20th century, has all carried out the research of nosema locustae both at home and abroad successively.But existing nosema locustae is introduced from the U.S., belongs to alien species, and it is bigger to use the back ecological risk; In addition, existing nosema locustae product is lower to the specificity of locust, the prevention effect instability, and resistance is relatively poor, and a little less than high temperature resistance and the ultraviolet ability, and production cost is higher.Therefore be necessary to develop and a kind ofly be applicable to that the native country is used and to grassland grasshopper and the strong strain of farmland migratory locusts specificity, it is stable to significantly improve control locust effect and preventive effect, can produce cheaply and use again simultaneously.
Summary of the invention
The object of the invention is to provide a kind of separation from the intravital microspore strain of car locust (Paranosema (Nosema) locustae), and this strain cording has very strong specificity, respectively grassland grasshopper and farmland migratory locusts is had the specificity lethal effect, and lethal effect is better.
Another object of the present invention provides a kind of purposes of microspore strain.
Separation of the present invention is from the intravital microspore strain of car locust (Paranosema (Nosema) locustae), this strain is that spore is scissiparity, size is about the 2-7 micron, strong stress resistance, preserving number is CGMCC No.2282, depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center, and the preservation place is in China. Beijing. and Zhong Guan-cun, December 5 2007 preservation time.
Separation of the present invention can be used for preventing and treating locust from the intravital microspore strain of car locust, for example farmland migratory locusts, grassland grasshopper, car locust, bamboo locust, oryza chinensis etc.
Compared with prior art, little spore product of the present invention has following advantage:
A. little spore product of the present invention is to follow the tracks of relatively to screen in the locust body in native country, uses the back to the rarer intervention effect of the ecosystem.
B. little spore of the present invention has very strong specificity, respectively grassland grasshopper and farmland migratory locusts is had the specificity lethal effect, and lethal effect is better, as inoculates 5 * 10
6The mortality ratio of spore 3 all back grassland grasshoppers and farmland migratory locusts reaches 95.27% and 91.38% respectively, is far longer than the pathogenic of existing nosema locustae.
C. the little spore that the present invention relates to is respectively at the controls of grassland grasshopper and farmland migratory locusts, and tailored version little spore in grassland has uvioresistant, be fit to the aircraft control and grassland grasshopper had characteristics such as sucking action, and is better to the grassland grasshopper prevention effect; The farmland little spore of migratory locusts tailored version has high temperature resistant and migratory locusts are had characteristics such as sucking action, and is better to the migratory locusts prevention effect.
D. little spore of the present invention is separated in car locust body, but not existing nosema locustae is isolating from deceiving in the locust body.
E. little spore production technology of the present invention is simple, and after the homogenate, 2000 rev/mins of separation get final product.Little spore strain of the present invention through resistance screening and processing, has significantly improved its resistance, particularly high temperature resistance and anti-uv-ray and has improved greatly; The little spore manufacturing requirements of the present invention is simple, and the breeding host obtains easily, helps mass production and application.
Description of drawings
Fig. 1 is the infection conditions of migratory locusts in the different year treatment samples district, anti-back.
Fig. 2 is the infection conditions of clouding car locust nature population in the different year treatment samples district, anti-back.
Fig. 3 is the cultivating technique figure of little spore of the present invention.
Fig. 4 is that little spore is 1.5 * 10
10The regulation effect of treatment zone during/ha concentration.
Fig. 5 is little spore 7.5 * 10
10The regulation effect of treatment zone during/ha concentration.
Embodiment
Below the present invention is described in detail by embodiment.
One, little spore (Paranosema locustae) screening:
1. trial-product is
Adopt the spore separation inoculation method, to field acquisition to susceptible locust separate, purifying, obtain little spore strain of 10 different sourcess, be inoculated into respectively again in different types of locust body, locust is raised under conditions suitable.
2. worm source
The worm source mainly is migratory locusts Locusta Migratoria and car locust Gastrimargusmarmoratus.
3. experimental population
3.1 laboratory population
Be collected in the field, and set up experimental population at the experiment indoor feeding.The raising condition is temperature 28-35 ℃, relative humidity 60-80%, illumination 12: 12.Feed the fresh corn seedling every day.
3.2 field population
Under field conditions (factors), the population of self-sow growth.
4. inoculation
Indoor employing 5 * 10
6The concentration inoculation, the field adopts 2 * 10
9Concentration sprays.
5. spore separation purifying
With susceptible locust homogenate, filter the back centrifugation, obtain spore liquid, be in store for
6. screening
6.1 spore collection
Wherein, Fig. 1 is the infection conditions of migratory locusts in the different year treatment samples district, anti-back.Fig. 2 is the infection conditions of clouding car locust nature population in the different year treatment samples district, anti-back.Gather susceptible locust every year from the field, and isolate its intravital little spore, concentrates the back storage, as the laboratory experiment inoculating spores, measures its toxicity to locust.
6.2 toxicity test
6.2.1 car locust body Endospore virulence variation situation
Isolated spore is to the following table 1 of the virulence analytical results of locust in car locust body.
Table 1
| Spore type (several years spores) | 1 |
2 years spores | 4 years spores | 7 years spores | 8 years spores | 9 years spores | 10 years spores |
| Migratory locusts | 68.21fg | 65.49de | 78.37b | 69.21cd | 91.38a | 65.88ef | 71.28bc |
| The car locust | 58.39g | 71.22de | 81.21bc | 71.25cd | 81.78b | 95.27a | 69.32ef |
Annotate: data are the mortality ratio in 3 weeks behind the little spore of inoculation in the table, and alphabetical identical table differential is different not remarkable, and alphabetical different table differential is different significantly.
The 8th year isolated spore has higher toxicity to migratory locusts as can be seen from Table 1, and isolated spore had control action kou preferably to the car locust in the 9th year.
6.2.2 migratory locusts body Endospore virulence variation situation
Isolated spore is shown in as the following table 2 the virulence analytical results of locust in the migratory locusts body.
Table 2
| Spore type (several years spores) | 1 |
2 years spores | 4 years spores | 7 years spores | 8 years spores | 9 years spores | 10 years spores |
| Migratory locusts | 88.31a | 85.24a | 88.55a | 89.49a | 91.08a | 87.64a | 91.25a |
| The car locust | 88.51a | 87.27a | 89.38a | 91.87a | 91.17a | 92.31a | 89.38a |
Annotate: data are the force of mortality in 3 weeks behind the little spore of inoculation in the table, and alphabetical identical table differential is different not remarkable, and alphabetical different table differential is different significantly.
The isolated spore of different year does not have significant difference to locust toxicity from showing as can be seen, and spore is more stable in the migratory locusts body.
So adopt the interior isolated 8 years spores of car locust body as the advantage strain, the control migratory locusts; Adopt isolated 9 years spore (P9) advantage strains in the car locust body, prevent and treat native locust (the car locust is a kind of of native locust).Identify through the U.S. little spore expert Leellen, think little spore (Paranosema (Nosema) locustae) new lines.Spore is more stable in the migratory locusts body, and we adopt the host of migratory locusts as these two kinds of advantage strains of breeding.The cultivating technique schema of this microspore strain is referring to Fig. 3.
Two, 8 years spores (P8) of two kinds of advantage strains and 9 years spore (P9) the cultivating technique of little spore (Paranosemalocustae):
1. sporogony
Inoculate P8 or P9 3 length of times migratory locusts, inoculum density is 5 * 10
5, vaccinization twice is put into the insectary again and is normally raised, and allows spore at the locust proliferation in vivo.
2. spore separation purifying
Susceptible locust is put into homogenizer homogenate, filter, with 2000 rev/mins speed centrifugal 20 minutes, obtain the spore of purifying with medical nylon gauze.
3. spore content check
Adopt medical blood cell counting plate, calculate spore quantity and concentration at microscopically, formula is as follows:
Total spore count=A/5 * 25 * 10000 * B in the 1mL spore liquid
Total spore count of lattice in A:5
B: extension rate
4. preparation processing
With the big wheat bran thorough mixing of spore liquid according to the ordinary skill in the art and water or 2mm, stir evenly, the adding trehalose is a small amount of, is made into preparation, is used to prevent and treat locust.
5. use technology
When locust density is hanged down, directly use microspore preparation, when locust density was high, microspore preparation intersected with the stuck gram of insect growth regulator(IGR) and sprays, all the may command locust disaster.4 weeks after the locust district uses the little spore 60ml/ha of P8, to proofread and correct the insect population rate of going down and reach more than 95%, the average correction insect population rate of going down reaches 63.87%, singly uses little spore, and infection rate is the highest remains Asiatic migrotory locust.Concrete data are referring to table 3.
Table 3
3 weeks after grassland locust district uses the little spore of P9, to proofread and correct the insect population rate of going down and reach more than 70%, the susceptible rate of survival locust is up to more than 60%.Concrete data are referring to table 4.And little spore concentration is 1.5 * 10
10/ ha and 7.5 * 10
10Regulation effect figure during/ha is shown in respectively among Fig. 4 and Fig. 5.
Table 4
Although above the specific embodiment of the present invention has been given to describe in detail and explanation; but should indicatedly be; we can carry out various equivalences to above-mentioned embodiment according to conception of the present invention and change and modification; when the function that it produced does not exceed spiritual that specification sheets and accompanying drawing contain yet, all should be within protection scope of the present invention.
Claims (2)
1. a separation is from the intravital microspore strain of car locust (Paranosema (Nosema) locustae), this strain is that microsporidium is scissiparity, the spore size is the 2-7 micron, preserving number is CGMCC No.2282, depositary institution is China Committee for Culture Collection of Microorganisms common micro-organisms center, the preservation place is in China. Beijing. and Zhong Guan-cun, December 5 2007 preservation time.
2. the purposes of the described microspore strain of claim 1 is characterized in that, microspore strain is used to prevent and treat locust.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101156045A CN101328462B (en) | 2008-06-25 | 2008-06-25 | Microspore strain and use thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101156045A CN101328462B (en) | 2008-06-25 | 2008-06-25 | Microspore strain and use thereof |
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| Publication Number | Publication Date |
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| CN101328462A CN101328462A (en) | 2008-12-24 |
| CN101328462B true CN101328462B (en) | 2010-08-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451100A (en) * | 2013-08-28 | 2013-12-18 | 河北晟普生物科技有限公司 | Locust microspore chorthippus strain and applications thereof |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250772B (en) * | 2011-06-24 | 2013-06-05 | 中国农业大学 | Antonospora locustae strain and application thereof in prevention and control of locust |
| CN103392676A (en) * | 2013-08-23 | 2013-11-20 | 新疆师范大学 | Nosema locustae inoculation method |
| CN109169645B (en) * | 2018-09-26 | 2020-09-04 | 中国农业大学 | Synergist for locust microspore biological preparation |
| CN112708565A (en) * | 2020-08-28 | 2021-04-27 | 北京中维新兴技术有限公司 | Locust microsporidia strain and preparation and use method thereof |
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2008
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103451100A (en) * | 2013-08-28 | 2013-12-18 | 河北晟普生物科技有限公司 | Locust microspore chorthippus strain and applications thereof |
| CN103451100B (en) * | 2013-08-28 | 2015-06-17 | 河北晟普生物科技有限公司 | Locust microspore chorthippus strain and applications thereof |
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