CN109287664A - A kind of Pesticidal combination and preparation method thereof containing beauveria bassiana and d ichlorbenzuron - Google Patents
A kind of Pesticidal combination and preparation method thereof containing beauveria bassiana and d ichlorbenzuron Download PDFInfo
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- CN109287664A CN109287664A CN201811372635.9A CN201811372635A CN109287664A CN 109287664 A CN109287664 A CN 109287664A CN 201811372635 A CN201811372635 A CN 201811372635A CN 109287664 A CN109287664 A CN 109287664A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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Abstract
The invention discloses a kind of Pesticidal combination containing beauveria bassiana and d ichlorbenzuron comprising common agricultural insecticide dosage form is made wherein being added with suitable agricultural insecticidal agent aid in the effective component being made of beauveria bassiana and d ichlorbenzuron.The present invention is by rationally promoting substance witchweed lactone using the chemical pesticide d ichlorbenzuron of low dosage and beauveria bassiana spore germination, beauveria bassiana is significantly promoted to the quick-acting of crop pest and whole control efficiency, the use of chemical pesticide can be effectively reduced, ecological disruption and environmental pollution are reduced, and crop yield and quality can be improved.
Description
Technical field
The invention belongs to agricultural insecticide fields, and in particular to a kind of Pesticidal combination of beauveria bassiana and d ichlorbenzuron.
Background technique
From the nineties in last century, with the increasingly raising that the mankind require environment and agricultural product quality and safety, agriculture is forced
Medicine must develop to low toxicity, nuisanceless direction, and biological pesticide is exactly a kind of green agriculture for meeting above-mentioned requirements, being compatible with the environment
Medicine.Beauveria bassiana is a kind of ascomycetous entomogenous fungi, can invade the polypide of 6 Ge Mu, 15 section, 200 various insects, mite class
Interior mass propagation, while generating white stiff plain (Non-ribosomal peptide toxoid), oosporein (benzoquinones toxoid) and calcium oxalate knot
Crystalline substance causes insect to be poisoned, and upsets metabolism so that dead.The widely dispersed of beauveria bassiana, from several meters of height above sea level to
More than 2000 meters of high mountain found the presence of beauveria bassiana, and main species include beauveria bassiana and brinell ball beauveria bassiana
Deng, it is normal that conidium is generated by vegetative propagation, it breeds fastly, is easy to cultivate.At present China registration with beauveria bassiana spore
Have 22 kinds for the pesticide species of effective component.As most biological control medicaments, beauveria bassiana currently on the market is killed
The worm agent problem that also generally existing quick-acting is poor, whole control efficiency is bad, cause its on the market be difficult to promote in production
And large-area applications.
D ichlorbenzuron is the product of China's original creation exploitation, is closed first by Hormone Inst., Jiangsu Prov and University Of Suzhou within 1976
At the analog of a chlorine substituted urea.Adopted name: d ichlorbenzuron (chlorbenzuron, mieyuniao) also known as Diflubenzuron Ⅲ,
Soviet Union's urea I, monochloro-benzene are grand.D ichlorbenzuron category benzoyl area kind Chitin synthesis inhibitor is insect hormone, makees
With mechanism be by inhibit insect cuticle chitin synthetase and urinate nucleosides coenzyme activity, come inhibit insect chitin synthesis from
And cause insect that cannot normally cast off a skin and dead, it can also influence DNA and albumen in the respiratory metabolism and embryo development procedure of ovum
Matter metabolism, makes in ovum larva lack chitin and dead immediately after cannot hatching or hatching, and applies in larval phase, makes the new table of pest
Skin formation is obstructed, delayed development, or lacks hardness, cannot normally be casted off a skin and be caused the lopsided pupa of dead or formation dead, therefore is right
Abnormal insect, especially lepidopterous larvae show as good insecticidal activity.
Summary of the invention
In order to improve beauveria bassiana insecticide quick-acting and control efficiency, while reducing the use of chemical pesticide d ichlorbenzuron
Amount, the invention proposes a kind of novel pesticide compositions.Beauveria bassiana and d ichlorbenzuron are carried out reasonable combination by the present invention, are filled
Divide the husking impact effect using d ichlorbenzuron to abnormal insect, while the promotion beauveria bassiana spore germination suitable by application
Substance improves parasitic ability of the beauveria bassiana on prevention and treatment target insect body, makees to greatly improve beauveria bassiana prevention and treatment
The quick-acting of object pest and whole control efficiency, have that quick-acting is good, the lasting period is long in actual use, and control efficiency is good, can
Various crop insect pest is effectively prevented, and chemical pesticide dosage total on unit area is made to decline to a great extent, to realize economical, high
Effect, the goal of the invention of environmental protection.
Pesticidal combination containing beauveria bassiana and d ichlorbenzuron of the invention, including be made of beauveria bassiana and d ichlorbenzuron
Effective component.
Pesticidal combination containing beauveria bassiana and d ichlorbenzuron of the invention, wherein beauveria bassiana spore content be 1 ×
107~1 × 1010 Spore/g, preferred beauveria bassiana spore content are 1 × 1010 Spore/g.
Pesticidal combination containing beauveria bassiana and d ichlorbenzuron of the invention, wherein the mass fraction of d ichlorbenzuron be 1%~
50%, the mass fraction of preferred d ichlorbenzuron is 5%~15%.
Pesticidal combination containing beauveria bassiana and d ichlorbenzuron of the invention, the witchweed lactone containing suitable concentration.
Pesticidal combination containing beauveria bassiana and d ichlorbenzuron of the invention passes through the suitable common agriculture of Integrated Selection optimization
Medicine auxiliary agent is prepared into dispersible oil-suspending agent, wettable powder, micro-capsule suspension or water-dispersible granules, preferred agriculture
Pharmaceutically dosage form is dispersible oil-suspending agent and wettable powder.
When Pesticidal combination containing beauveria bassiana and d ichlorbenzuron of the invention is prepared into oil-suspending agent: oily phase carrier is selected
Rapeseed oil, epoxidized soybean oil, soybean oil, peanut oil, methyl oleate etc., preferred oil phase carrier is rapeseed oil and epoxy soybean
Oil；Surfactant package selects castor oil-ethylene-oxide condensation product (EL series), fatty alcohol and ethylene oxide condensate (O system
Column), fatty acid and ethylene oxide condensate (SG series), polyoxyethylene sorbitan fatty acid ester (T series), dodecyl
Benzene sulfonic acid calcium, preferred surfactant package are nonionic surfactant castor oil-ethylene-oxide condensation product (EL series)
It is combined with polyoxyethylene sorbitan fatty acid ester (T series) with anionic surfactant calcium dodecyl benzene sulfonate；Increase
Thick dose of selection activated silicic acid magnalium, xanthan gum, organobentonite, preferred thickener are that aluminium-magnesium silicate is combined with xanthan gum；Disappear
Infusion selects organic silicon defoamer.Preparation method is by the curable type such as effective component d ichlorbenzuron, thickener auxiliary agent in oily phase carrier
In ground by sand mill, partial size reaches 2~3 microns, and remaining auxiliary material is added by formula, beauveria bassiana spore is then added
Sub- powder is adjusted to and requires concentration, sufficiently shears, stirs evenly, obtains finished product.
When Pesticidal combination containing beauveria bassiana and d ichlorbenzuron of the invention is prepared into wettable powder agent: filler is selected
Cornstarch, kaolin, white carbon black, attapulgite, diatomite etc., preferred filler is cornstarch and daytime is black, kaolin
Combination；Surfactant package selects dispersing agent MF, nonylphenol polyoxyethylene ether formaldehyde condensation products sodium sulphate
Salt, sodium lignin sulfonate, neopelex, calcium lignosulfonate, preferred surfactant package are sulfomethylated lignins
Sour calcium is combined with dispersing agent MF.Preparation method is to mix effective component d ichlorbenzuron with filler, auxiliary agent
It is even, beauveria bassiana spore is added after air-flow crushing and is adjusted to required concentration, is sufficiently mixed uniformly, obtains finished product.
Pesticidal combination containing beauveria bassiana and d ichlorbenzuron of the invention can be used for preventing and treating striped rice borer, corn borer, twill
The insect pests such as noctuid, bollworm.
Technical solution
The present invention passes through system research beauveria bassiana in 2~3 age of the striped rice borer children of the horizontal d ichlorbenzuron processing of various concentration first
Situation is infected on worm polypide, minimum d ichlorbenzuron concentration for the treatment of and suitable beauveria bassiana spore concentration is filtered out, is fitted
Suitable effective component beauveria bassiana is combined with d ichlorbenzuron；Then by studying different oily phase carrier or filler to the white deadlock of ball spore
The suitable combination of oily phase carrier or filler combination are screened in the influence of bacterium spore germination；According to the oily phase carrier or filler group of screening
The characteristic with combination of active principles mixture is closed, suitable auxiliary combination is screened, prepares contrivance sample；The suitable ball of screening
Beauveria bassiana spore germination promotes substance witchweed dermolide concentrations, is verified, is advanced optimized by indoor biometrics and field efficacy
Proportion forms product formula.
Relationship of the beauveria bassiana to striped rice borer infection ability and beauveria bassiana spore concentration and d ichlorbenzuron concentration level
It is 1 × 10 that beauveria bassiana spore concentration, which is arranged,4、1×105、1×106With 1 × 107 Tetra- levels of spore/g, setting are gone out
Young urea concentration is 0,15.625,31.25,62.5, six 125,250ppm levels, and totally 24 are handled, 20 cephalonts of every processing, 4 times
It repeats.It selects consistent 2~3 instar larvae of striped rice borer of growth first to be handled with d ichlorbenzuron using dip method, uses ball after polypide is dry
The processing of beauveria bassiana spore suspension, 24 as a child detect larva bacterial bearing rate.It the results are shown in Table 1.It goes out as it can be seen from table 1 being added
After young urea, infection rate of the beauveria bassiana on 2~3 instar larvae of striped rice borer, corn borer and prodenia litura is significantly improved；D ichlorbenzuron
Concentration for the treatment of reaches 6.25ppm, and beauveria bassiana spore concentration reaches 1 × 106When, beauveria bassiana is in striped rice borer, corn borer 2
Infection rate on~3 instar larvaes is respectively 78.75% and 82.50%, and the infection rate on 2~3 instar larvae of prodenia litura is
52.50%；Beauveria bassiana spore concentration reaches 1 × 106And after d ichlorbenzuron concentration for the treatment of reaches 6.25ppm, with the white deadlock of ball spore
The raising of bacterium spore concentration and d ichlorbenzuron concentration for the treatment of, Infected by Beauveria bassiana rate increase not significant.The white deadlock of preferred ball spore
Bacterium spore concentration is 1 × 106, the concentration of d ichlorbenzuron is 6.25-12.5ppm.
Relationship of 1 beauveria bassiana of table to striped rice borer infection ability and d ichlorbenzuron concentration level
Relationship of 2 beauveria bassiana of table to corn borer infection ability and d ichlorbenzuron concentration level
Relationship of 3 beauveria bassiana of table to prodenia litura infection ability and d ichlorbenzuron concentration level
The influence of oily phase carrier or filler to beauveria bassiana spore germination rate
Common oily phase carrier and filler are selected, its influence to beauveria bassiana spore germination rate is studied, as a result such as table 4.From table 4
Know, it is relatively small to beauveria bassiana spore germination rate to commonly use rapeseed oil and epoxidized soybean oil in oil phase carrier；Customary filler pair
Beauveria bassiana spore germination rate is smaller, and wherein the influence of cornstarch and white carbon black is little, and cornstarch is to spore germination
Also show certain facilitation.Preferred oil phase carrier is rapeseed oil, and preferred filler is cornstarch and white carbon black group
It closes.
The influence of table 4 oily phase carrier or filler to beauveria bassiana spore germination rate
Specific embodiment
Below with reference to embodiment, the invention will be further described, but essence of the invention is not limited to following embodiments.
Embodiment 1
The preparation method of 1000000000 beauveria bassiana, 12.5% d ichlorbenzuron oil-suspending agent: by mass percentage.
Beauveria bassiana 1 × 109Spore/g；
D ichlorbenzuron 12.5%；
EL10 6%；
T-40 4 %；
Calcium dodecyl benzene sulfonate 4%；
Witchweed lactone 0.03%；
Organic silicon defoamer 0.5%；
Aluminium-magnesium silicate 0.5%
Xanthan gum 0.1%
Rapeseed oil surplus.
Preparation method: according to formula, the curable type such as effective component d ichlorbenzuron, thickener auxiliary agent is passed through into sand in oily phase carrier
Grinding machine grinding, partial size reach 2~3 microns, and remaining auxiliary material is added by formula, beauveria bassiana conidia powder is then added, adjusts
Section is sufficiently sheared, is stirred evenly, obtain finished product to concentration is required.
Embodiment 2
The preparation method of 2000000000 beauveria bassiana, 12.5% d ichlorbenzuron oil-suspending agent: by mass percentage.
Beauveria bassiana 2 × 109Spore/g；
D ichlorbenzuron 12.5%；
EL10 6%；
T-40 4 %；
Calcium dodecyl benzene sulfonate 4%；
Witchweed lactone 0.03%；
Organic silicon defoamer 0.5%；
Aluminium-magnesium silicate 0.5%
Xanthan gum 0.1%
Rapeseed oil surplus.
Preparation method, with embodiment 1.
Embodiment 3
The preparation method of 4000000000 beauveria bassiana, 12.5% d ichlorbenzuron oil-suspending agent: by mass percentage.
Beauveria bassiana 4 × 109Spore/g；
D ichlorbenzuron 12.5%；
EL10 6%；
T-40 4 %；
Calcium dodecyl benzene sulfonate 4%；
Witchweed lactone 0.03%；
Organic silicon defoamer 0.5%；
Aluminium-magnesium silicate 0.5%
Xanthan gum 0.1%
Rapeseed oil surplus.
Embodiment 4
The preparation method of 2000000000 beauveria bassiana, 6.25% d ichlorbenzuron oil-suspending agent: by mass percentage.
Beauveria bassiana 2 × 109Spore/g；
D ichlorbenzuron 6.25%；
EL10 6%；
T-40 4 %；
Calcium dodecyl benzene sulfonate 4%；
Witchweed lactone 0.03%；
Organic silicon defoamer 0.5%；
Aluminium-magnesium silicate 0.5%
Xanthan gum 0.1%
Rapeseed oil surplus.
Preparation method, with embodiment 1.
Embodiment 5
The preparation method of 4000000000 beauveria bassiana, 6.25% d ichlorbenzuron oil-suspending agent: by mass percentage.
Beauveria bassiana 4 × 109Spore/g；
D ichlorbenzuron 6.25%；
EL10 6%；
T-40 4 %；
Calcium dodecyl benzene sulfonate 4%；
Witchweed lactone 0.03%；
Organic silicon defoamer 0.5%；
Aluminium-magnesium silicate 0.5%
Xanthan gum 0.1%
Rapeseed oil surplus.
Preparation method, with embodiment 1.
Embodiment 6
The preparation method of 2000000000 beauveria bassiana, 6.25% d ichlorbenzuron wettable powder: by mass percentage.
Beauveria bassiana 2 × 109Spore/g；
D ichlorbenzuron 6.25%；
Calcium lignosulfonate 5%；
Dispersing agent MF 2%；
Witchweed lactone 0.03%；
White carbon black 10%
Cornstarch surplus.
Preparation method: according to formula, effective component d ichlorbenzuron is uniformly mixed with filler, auxiliary agent, is added after air-flow crushing
Beauveria bassiana spore is simultaneously adjusted to required concentration, is sufficiently mixed uniformly, obtains finished product.
Embodiment 7
The preparation method of 2000000000 beauveria bassiana, 12.5% d ichlorbenzuron wettable powder: by mass percentage.
Beauveria bassiana 2 × 109Spore/g；
D ichlorbenzuron 12.5%；
Calcium lignosulfonate 5%；
Dispersing agent MF 3%；
Witchweed lactone 0.03%；
White carbon black 10%；
Cornstarch surplus.
Preparation method, with embodiment 6.
The preparation method of 1 12.5% d ichlorbenzuron oil-suspending agent (being not added with beauveria bassiana) of reference examples: by mass percentage.
D ichlorbenzuron 12.5%；
EL10 6%；
T-40 4 %；
Calcium dodecyl benzene sulfonate 4%；
Organic silicon defoamer 0.5%；
Aluminium-magnesium silicate 0.5%
Xanthan gum 0.1%
Rapeseed oil surplus.
Preparation method, with embodiment 1.
The preparation side of 2 20 hundred million beauveria bassiana of reference examples, 12.5% d ichlorbenzuron oil-suspending agent (being not added with witchweed lactone)
Method: by mass percentage.
Beauveria bassiana 2 × 109Spore/g；
D ichlorbenzuron 12.5%；
EL10 6%；
T-40 4 %；
Calcium dodecyl benzene sulfonate 4%；
Organic silicon defoamer 0.5%；
Aluminium-magnesium silicate 0.5%
Xanthan gum 0.1%
Rapeseed oil surplus.
Preparation method, with embodiment 1.
The preparation method of 3 20 hundred million beauveria bassiana oil-suspending agent (being not added with d ichlorbenzuron) of reference examples: by mass percentage.
Beauveria bassiana 2 × 109Spore/g；
EL10 6%；
T-40 4 %；
Calcium dodecyl benzene sulfonate 4%；
Witchweed lactone 0.03%；
Organic silicon defoamer 0.5%；
Aluminium-magnesium silicate 0.5%
Xanthan gum 0.1%
Rapeseed oil surplus.
Preparation method, with embodiment 1.
Reference examples 4
The preparation method of 12.5% d ichlorbenzuron wettable powder (being not added with beauveria bassiana): by mass percentage.
D ichlorbenzuron 12.5%；
Calcium lignosulfonate 5%；
Dispersing agent MF 3%；
White carbon black 10%；
Cornstarch surplus.
Preparation method, with embodiment 6.
Reference examples 5
The preparation method of 2000000000 beauveria bassiana, 12.5% d ichlorbenzuron wettable powder (being not added with witchweed lactone): by quality hundred
Divide ratio.
Beauveria bassiana 2 × 109Spore/g；
D ichlorbenzuron 12.5%；
Calcium lignosulfonate 5%；
Dispersing agent MF 3%；
White carbon black 10%；
Kaolin surplus.
Preparation method, with embodiment 6.
Reference examples 6
The preparation method of 2000000000 beauveria bassiana wettable powders (being not added with d ichlorbenzuron): by mass percentage.
Beauveria bassiana 2 × 109Spore/g；
Calcium lignosulfonate 5%；
Dispersing agent MF 3%；
Witchweed lactone 0.03%；
White carbon black 10%；
Kaolin surplus.
Preparation method, with embodiment 6.
Product of the present invention is described further with the toxicity of embodiment and field efficacy experiment result below.
5 embodiment of table and reference examples effect measuring
Spore germination rate measurement, indoor Infected by Beauveria bassiana rate measurement, the death rate have been carried out to 2000 times of liquid of embodiment 1-7
Measurement and field control effect measurement, as a result such as table 5.As can be seen from Table 5, embodiment 1-7 is to striped rice borer, corn borer and twill
Noctuid has a preferable control efficiency, embodiment 2-7 to the field efficacy of striped rice borer and corn borer 80% or more, to twill
The field efficacy of noctuid is 70% or more.Embodiment is compared with reference examples 1 and reference examples 4 as can be seen that the control efficiency of embodiment
It is mainly derived from beauveria bassiana, embodiment is compared with reference examples 3, reference examples 6 as can be seen that the d ichlorbenzuron of doses is added
Beauveria bassiana can effectively be promoted to the control efficiency of striped rice borer；Embodiment can be seen that compared with reference examples 2 and reference examples 4
Witchweed lactone has good facilitation to beauveria bassiana spore germination, also has to field control effect and significantly mentions
It rises.Therefore, present invention proposition is compounded with the d ichlorbenzuron of low dosage with beauveria bassiana, is promoted by beauveria bassiana spore germination
The application of substance, and dosage form optimization design is carried out to invention product, reach and striped rice borer, corn are effectively prevented with beauveria bassiana
The effect of snout moth's larva and prodenia litura makes the usage amount of chemical pesticide d ichlorbenzuron reduce 50% or more, has broad application prospects.
Claims (11)
1. a kind of Pesticidal combination containing beauveria bassiana and d ichlorbenzuron, it is characterised in that: in the Pesticidal combination, ball spore
Beauveria bassiana spore content is 1 × 107~1 × 1010 Spore/g, the mass fraction of d ichlorbenzuron are 1%~50%, beauveria bassiana spore
Son, which is sprouted, promotes substance 0.01-0.1%.
2. Pesticidal combination according to claim 1, it is characterised in that: in the Pesticidal combination, beauveria bassiana
Spore content is 1 × 109~1 × 1010 Spore/g, the mass fraction of d ichlorbenzuron are 3%~30%, beauveria bassiana spore germination
Promote substance 0.01-0.05%.
3. Pesticidal combination according to claim 2, it is characterised in that: in the Pesticidal combination, beauveria bassiana
Spore content is 1 × 109 Spore/g, the mass fraction of d ichlorbenzuron are 5%~15%, and beauveria bassiana spore germination promotes substance
0.03%。
4. Pesticidal combination according to claim 1, it is characterised in that: in the Pesticidal combination, added with appropriate
Agricultural insecticidal agent aid, common agricultural insecticide dosage form is made.
5. Pesticidal combination according to claim 4, it is characterised in that: dosage form can be made in the Pesticidal combination
For oil-suspending agent, wettable powder.
6. Pesticidal combination described in -5 according to claim 1, it is characterised in that: the bactericidal composition is prepared into oily suspension
When agent, auxiliary agent used is surfactant, thickener, oily phase carrier.
7. Pesticidal combination described in -5 according to claim 1, it is characterised in that: the Pesticidal combination is prepared into wettable
When pulvis, auxiliary agent used is surfactant, filler.
8. Pesticidal combination described in -7 according to claim 1, it is characterised in that: the beauveria bassiana spore germination promotes
Substance is witchweed lactone；The surfactant is dispersing agent MF, sodium lignin sulfonate, dodecyl
Benzene sulfonic acid sodium salt, calcium lignosulfonate, calcium dodecyl benzene sulfonate, castor oil-ethylene-oxide condensation product (EL series), fatty alcohol with
Ethylene oxide condensate (O series), polyoxyethylene sorbitan fatty acid ester (T series)；The thickener be xanthan gum,
The mixing of one or both of aluminium-magnesium silicate, organobentonite；The oily phase carrier is soybean oil, peanut oil, rapeseed oil, oil
The combination of one or more of sour methyl esters and epoxidized soybean oil；The filler is diatomite, kaolin, attapulgite, white
The combination of one or more of carbon black, cornstarch.
9. Pesticidal combination described in -8 according to claim 1, it is characterised in that: a kind of killing containing beauveria bassiana and d ichlorbenzuron
The method that worm composition is prepared into oil-suspending agent is to lead to the curable type such as effective component d ichlorbenzuron, thickener auxiliary agent in oily phase carrier
Sand mill grinding is crossed, partial size reaches 2~3 microns, and remaining auxiliary material is added by formula, beauveria bassiana spore is then added
Powder is adjusted to and requires concentration, sufficiently shears, stirs evenly, obtains finished product.
10. Pesticidal combination described in -8 according to claim 1, it is characterised in that: a kind of containing beauveria bassiana and d ichlorbenzuron
The method that Pesticidal combination is prepared into wettable powder is to be uniformly mixed effective component d ichlorbenzuron with filler, auxiliary agent, air-flow powder
Beauveria bassiana spore is added after broken and is adjusted to required concentration, is sufficiently mixed uniformly, obtains finished product.
11. the described in any item Pesticidal combinations of claim 1-10 are sprayed after being diluted with water 1000~2000 times using constant
Mist, for preventing and treating the insect pests such as striped rice borer, corn borer, prodenia litura, bollworm.
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| CN201811372635.9A CN109287664A (en) | 2018-11-19 | 2018-11-19 | A kind of Pesticidal combination and preparation method thereof containing beauveria bassiana and d ichlorbenzuron |
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| CN201811372635.9A Pending CN109287664A (en) | 2018-11-19 | 2018-11-19 | A kind of Pesticidal combination and preparation method thereof containing beauveria bassiana and d ichlorbenzuron |
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| CN115316404A (en) * | 2022-09-06 | 2022-11-11 | 浙江宜葆生物科技有限公司 | Dispersible oil suspending agent containing pyrethrin and beauveria bassiana and application thereof |
| CN115316404B (en) * | 2022-09-06 | 2023-09-29 | 浙江宜葆生物科技有限公司 | Dispersible oil suspension containing pyrethrin and beauveria bassiana and application thereof |
| CN115581237A (en) * | 2022-10-10 | 2023-01-10 | 天津农学院 | Insecticidal composition containing tolfenpyrad and beauveria bassiana and application thereof |
| CN115581237B (en) * | 2022-10-10 | 2024-03-01 | 天津农学院 | Insecticidal composition containing tolfenpyrad and beauveria bassiana and application thereof |
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