CN111837920A - Application method of novel compound in improving soil aluminum toxin resistance of plants - Google Patents
Application method of novel compound in improving soil aluminum toxin resistance of plants Download PDFInfo
- Publication number
- CN111837920A CN111837920A CN202010748901.4A CN202010748901A CN111837920A CN 111837920 A CN111837920 A CN 111837920A CN 202010748901 A CN202010748901 A CN 202010748901A CN 111837920 A CN111837920 A CN 111837920A
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- plants
- aluminum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an application method of a novel compound in improving soil aluminum toxicity resistance of plants, relates to the technical field of plant planting, and comprises the following steps. The novel compound is added into a culture dish for plant culture, the activity of SOD, POD and CAT of plants stressed by aluminum toxicity can be obviously enhanced, namely under normal growth and aluminum stress, the compound can obviously reduce the degree of oxidative damage of the plants, thereby effectively removing ROS caused by aluminum stress, lightening the damage of root systems, reducing the possibility of death and death of the plants due to aluminum toxicity, and further solving the problem that the plant growth and development are seriously affected by certain deficiency in the existing compound application method for treating and preventing the plant aluminum toxicity.
Description
Technical Field
The invention relates to the technical field of plant planting, in particular to an application method of a novel compound in improving soil aluminum toxin resistance of plants.
Background
Aluminum is the most abundant metal element in the earth crust, is ubiquitous in soil, and 30% of cultivated land and 50% of potential cultivated land all over the world are reported to be acid soil, so that aluminum toxicity caused by soil acidification is a hot problem of global concern at present, and aluminum mainly exists in insoluble forms such as alumina and aluminum silicate in neutral or alkaline soil and does not generate toxicity to plants; in acid soil, aluminum is trivalent aluminum ion (Al)3+) The compound is released into a soil solution, is combined with the cell wall of a plant root system and enters a cell symplast to inhibit mitosis and DNA synthesis, and blocks the absorption of water and nutrients, so that the growth and the function of the plant root are inhibited, the plant root is poisoned, and the root system is finally necrosed to influence the growth and development of the plant.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides an application method of a novel compound in improving the resistance of plants to soil aluminum toxicity, and solves the problem that the growth and development of plants are seriously influenced due to certain defects of the existing application method of the compound for treating and preventing the plant aluminum toxicity.
(II) technical scheme
In order to achieve the above purposes, the technical scheme adopted by the invention is as follows: the application method of the novel compound in improving the resistance of plants to soil aluminum toxicity comprises the following steps:
a1, selecting proper plants.
a2, injecting the compound product with melatonin and strigolactone as effective components into rooting culture medium.
a3, putting the selected plants into a culture medium for culturing.
Preferably, the plant is a monocotyledon or dicotyledon.
Preferably, the monocotyledon is wheat, corn, rice or leek; the dicotyledonous plant is statone, soybean, peanut or radish.
Preferably, the rooting medium is MS + 1/26-BA.
Preferably, the culture medium and the compound are added to each of the rooted plants at the same concentration.
(III) advantageous effects
The invention has the beneficial effects that:
the novel compound is added into a culture dish for plant culture, the activity of SOD, POD and CAT of plants stressed by aluminum toxicity can be obviously enhanced, namely under normal growth and aluminum stress, the compound can obviously reduce the degree of oxidative damage of the plants, thereby effectively removing ROS caused by aluminum stress, lightening the damage of root systems, reducing the possibility of death and death of the plants due to aluminum toxicity, and further solving the problem that the plant growth and development are seriously affected by certain deficiency in the existing compound application method for treating and preventing the plant aluminum toxicity.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: the application method of the novel compound in improving the resistance of plants to soil aluminum toxicity comprises the following steps:
a1, selecting proper plants.
a2, injecting the compound product with melatonin and strigolactone as effective components into rooting culture medium.
a3, putting the selected plants into a culture medium for culturing.
The plant is a monocot or a dicot.
The monocotyledon is wheat, corn, rice or Chinese chive; the dicotyledonous plant is statone, soybean, peanut or radish.
The rooting culture medium is MS + 1/26-BA.
The culture medium and the compound added into each rooting plant have the same concentration, wherein the compound is a mixed solution of melatonin and strigolactone in a proper proportion and concentration ratio.
Example one
The application method of the novel compound in improving the resistance of plants to soil aluminum toxicity comprises the following steps:
s1, selecting a mixed solution with a compound of melatonin and strigolactone in a proper proportion and concentration ratio, and filtering for sterilization.
And S2, performing a grouping control test, and dividing into four groups, wherein the group A is a normal rooting culture medium, the group B is a normal rooting culture medium added with the compound, the group C is a culture medium added with 100 mu mol/L aluminum chloride (PH 4.5), and the group D is a culture medium added with 100 mu mol/L aluminum chloride (PH 4.5) and the compound.
S3, subculturing plants for one month at the temperature of 25 ℃ in a culture room and under the light intensity of 3000lux, selecting 48 plant seedlings with consistent growth vigor, and transferring the plant seedlings into a rooting culture medium for a total treatment period of 45 days.
S4, measuring the total root length, total surface area and root tip number of each group of plant roots and the activity of SOD, CAT and POD of the roots after 45 days of treatment, wherein SOD refers to superoxide dismutase, CAT refers to catalase, POD refers to peroxidase and ROS refers to plant active oxygen.
And S4, obtaining a conclusion through statistical analysis.
The specific data are as follows:
the above-mentioned embodiments, objects, technical solutions and advantages of the present invention are further described in detail, it should be understood that the above-mentioned embodiments are only illustrative of the present invention and are not intended to limit the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. An application method of a novel compound in improving the resistance of plants to soil aluminum toxicity is characterized in that: the application method of the compound in improving the resistance of plants to soil aluminum toxicity comprises the following steps:
a1, selecting proper plants.
a2, injecting the compound product with melatonin and strigolactone as effective components into rooting culture medium.
a3, putting the selected plants into a culture medium for culturing.
2. The method of using a novel complex according to claim 1 for increasing the resistance of plants to soil aluminium toxicity, characterized in that: the plant is a monocotyledon or a dicotyledon.
3. The method of using a novel complex according to claim 2 for increasing the resistance of plants to soil aluminium toxicity, characterized in that: the monocotyledon is wheat, corn, rice or Chinese chive; the dicotyledonous plant is statone, soybean, peanut or radish.
4. The method of using a novel complex according to claim 1 for increasing the resistance of plants to soil aluminium toxicity, characterized in that: the rooting culture medium is MS + 1/26-BA.
5. The method of using a novel complex according to claim 1 for increasing the resistance of plants to soil aluminium toxicity, characterized in that: the concentration of the culture medium and the compound added into each rooting plant is the same.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010748901.4A CN111837920A (en) | 2020-07-30 | 2020-07-30 | Application method of novel compound in improving soil aluminum toxin resistance of plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010748901.4A CN111837920A (en) | 2020-07-30 | 2020-07-30 | Application method of novel compound in improving soil aluminum toxin resistance of plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111837920A true CN111837920A (en) | 2020-10-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN202010748901.4A Pending CN111837920A (en) | 2020-07-30 | 2020-07-30 | Application method of novel compound in improving soil aluminum toxin resistance of plants |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111826383A (en) * | 2020-07-16 | 2020-10-27 | 昆明理工大学 | Application of Danbo black soybean superoxide dismutase gene in improving plant aluminum tolerance |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109287664A (en) * | 2018-11-19 | 2019-02-01 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Pesticidal combination and preparation method thereof containing beauveria bassiana and d ichlorbenzuron |
| CN109640631A (en) * | 2016-06-17 | 2019-04-16 | 积水化学工业株式会社 | The method for improving plant salt tolerance |
| CN109940031A (en) * | 2019-03-20 | 2019-06-28 | 浙江大学 | Witchweed lactone is alleviating Cd stress to the application in plant generation cadmium poisoning |
| US20200048156A1 (en) * | 2018-08-09 | 2020-02-13 | Thomas T. Yamashita | Foliar Fertilizer Compositions and Methods for Using Same |
-
2020
- 2020-07-30 CN CN202010748901.4A patent/CN111837920A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109640631A (en) * | 2016-06-17 | 2019-04-16 | 积水化学工业株式会社 | The method for improving plant salt tolerance |
| US20200048156A1 (en) * | 2018-08-09 | 2020-02-13 | Thomas T. Yamashita | Foliar Fertilizer Compositions and Methods for Using Same |
| CN109287664A (en) * | 2018-11-19 | 2019-02-01 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of Pesticidal combination and preparation method thereof containing beauveria bassiana and d ichlorbenzuron |
| CN109940031A (en) * | 2019-03-20 | 2019-06-28 | 浙江大学 | Witchweed lactone is alleviating Cd stress to the application in plant generation cadmium poisoning |
Non-Patent Citations (2)
| Title |
|---|
| 吕婷: "褪黑素对小麦铝毒的缓解作用及其机理研究", 《中国优秀博硕士学位论文全文数据库硕士 农业科技辑》 * |
| 张灿: "外源独脚金内酯缓解大麦镉毒害的机理研究", 《中国优秀博硕士学位论文全文数据库硕士 工程科技Ⅰ辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111826383A (en) * | 2020-07-16 | 2020-10-27 | 昆明理工大学 | Application of Danbo black soybean superoxide dismutase gene in improving plant aluminum tolerance |
| CN111826383B (en) * | 2020-07-16 | 2022-08-02 | 昆明理工大学 | Application of Danbo black soybean superoxide dismutase gene in improving plant aluminum tolerance |
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Application publication date: 20201030 |
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