CN111802393A - Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof - Google Patents
Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof Download PDFInfo
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- CN111802393A CN111802393A CN202010748900.XA CN202010748900A CN111802393A CN 111802393 A CN111802393 A CN 111802393A CN 202010748900 A CN202010748900 A CN 202010748900A CN 111802393 A CN111802393 A CN 111802393A
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- compound
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- melatonin
- strigolactone
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
- Indole Compounds (AREA)
Abstract
The invention discloses a novel compound for improving soil aluminum toxicity resistance of plants and a preparation method thereof, relates to the technical field of plant planting, and discloses a novel substance of a product taking melatonin and strigolactone as effective components in improving aluminum stress resistance of plants. This improve plant and resist novel compound of soil aluminium toxicity and preparation method thereof, compound through the ratio can be obvious increase POD in the root system, the activity of CAT, the activity of SOD has been reduced, and then seedling root system damage under the aluminium stress has been reduced, under normal growth and aluminium stress promptly, the compound plant that melatonin and strigolactone were applyed to the external source receives the degree of oxidative damage and is obviously less than the root system of the compound plant that does not applyed, and then make the external source applyed melatonin and strigolactone compound can effectively clear away the ROS that arouses by the aluminium stress, thereby solved the problem that the aluminium ion is absorbed by the plant root system and causes the plant root system to be poisoned and arouse plant dystrophy, growth and poor and fruit output and quality decline.
Description
Technical Field
The invention relates to the technical field of plant planting, in particular to a novel compound for improving soil aluminum toxicity resistance of plants and a preparation method thereof.
Background
In recent years, the phenomenon of soil acidification in apple growing areas is becoming more and more serious due to unreasonable fertilization and management measures, in acid soil, aluminum ions are absorbed by plant roots to cause poisoning of the plant roots, which causes problems of plant nutrient deficiency, growth and development badness, fruit yield and quality reduction, etc., nowadays, quick lime is commonly used to relieve soil acidification and aluminum poisoning effects, but long-term application causes repeated soil acidification, therefore, while the traditional method is used for relieving the acidity of the soil, a new method is searched for reducing the damage of aluminum toxicity to plants, which has extremely important significance in agricultural production, however, no one researches the influence of two plant secondary metabolites on wood rooting under the condition of improving aluminum stress, therefore, a novel compound with high plant resistance to soil aluminum toxicity and a preparation method thereof are provided to solve the problem.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a novel compound for improving the soil aluminum toxicity resistance of plants and a preparation method thereof, and solves the problems of plant nutrition disorder, poor growth and development, and reduced fruit yield and quality caused by plant root poisoning caused by the absorption of aluminum ions by plant roots.
(II) technical scheme
In order to achieve the above purposes, the technical scheme adopted by the invention is as follows: a novel compound for improving the resistance of plants to soil aluminum toxicity and a preparation method thereof are provided, wherein the novel substance of the product taking melatonin and strigolactone as effective components is used for improving the resistance of plants to aluminum stress.
Preferably, the effective component of the compound is melatonin and strigolactone mixed solution.
Preferably, the concentration of the compound mixed solution is: melatonin is 10-100 mu mol/L, and strigolactone is 0.1-1 mu mol/L.
Preferably, the concentration of the compound aqueous solution is 10 mu mol/L melatonin and 1 mu mol/L strigolactone.
Preferably, the compound aqueous solution is prepared by diluting a melatonin ethanol solution of 10mmol/L and a strigolactone methanol solution of 1 mmol/L.
Preferably, the volume ratio of the melatonin to the strigolactone is 1-2: 1.
preferably, the volume ratio of the melatonin to the strigolactones is 1: 1.
(III) advantageous effects
The invention has the beneficial effects that:
this improve plant and resist novel compound of soil aluminium toxicity and preparation method thereof, compound through the ratio can be obvious increase POD in the root system, the activity of CAT, the activity of SOD has been reduced, and then seedling root system damage under the aluminium stress has been reduced, under normal growth and aluminium stress promptly, the compound plant that melatonin and strigolactone were applyed to the external source receives the degree of oxidative damage and is obviously less than the root system of the compound plant that does not apply, and then make the external source apply melatonin and strigolactone compound can effectively clear away the ROS that arouses by the aluminium, thereby it has alleviateed the root system damage and has solved the problem that the aluminium ion is absorbed by plant root system and cause plant root system poisoning to cause plant dystrophia, growth is bad and fruit yield and quality decline.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: a novel compound for improving the resistance of plants to soil aluminum toxicity and a preparation method thereof are provided, which are novel substances of products taking melatonin and strigolactone as effective components in improving the resistance of plants to aluminum stress.
The effective component of the compound is mixed solution of melatonin and strigolactone.
The concentration of the composite mixed solution is as follows: melatonin is 10-100 mu mol/L, and strigolactone is 0.1-1 mu mol/L.
The concentration of the compound aqueous solution is 10 mu mol/L melatonin and 1 mu mol/L strigolactone.
The compound water solution is prepared by diluting melatonin ethanol solution of 10mmol/L and strigolactone methanol solution of 1 mmol/L.
The volume ratio of melatonin to strigolactone is 1-2: 1.
the volume ratio of melatonin to strigolactone is 1: 1.
example one
A novel compound for improving the resistance of plants to soil aluminum toxicity and a preparation method thereof comprise the following steps:
s1, firstly, selecting 10mmol/L melatonin ethanol solution and 1mmol/L strigolactone methanol solution.
S2, adding the selected solution into a rooting culture medium according to the proportion of 1:1, and further preparing the rooting culture medium into a culture medium containing a novel compound of 10 mu mol/L melatonin and 1 mu mol/L strigolactone.
S3, selecting monocotyledons or dicotyledons, wherein the monocotyledons are wheat, corn, rice or leek, and the dicotyledons are gynura bicolor, soybean, peanut or radish.
S4, adding the prepared culture medium of the novel compound into the plant for use after suction filtration and sterilization.
Example two
A novel compound for improving the resistance of plants to soil aluminum toxicity and a preparation method thereof comprise the following steps:
s1, adding a 10mmol/L melatonin ethanol solution and a 1mmol/L strigolactone methanol solution into a rooting culture medium according to a ratio of 1:1, preparing a culture medium containing a novel compound of 10 mu mol/L melatonin and 1 mu mol/L strigolactone, and selecting suitable monocotyledon plants or dicotyledon plants.
And S2, making a grouping control test, and grouping into four groups, wherein the group A is a normal rooting culture medium, the group B is a normal rooting culture medium added with the compound, the group C is a culture medium added with 100 mu mol/L aluminum chloride (PH 4.5), and the group D is a culture medium added with 100 mu mol/L aluminum chloride (PH 4.5) and the compound.
S3, subculturing the four groups of plants for one month at the temperature of 25 ℃ in a culture room and under the light intensity of 3000lux to select plants, selecting 48M 26 tissue culture seedlings with consistent growth vigor, and transferring the seedlings into a rooting culture medium for a total treatment period of 45 days.
And S4, measuring the total root length, the total surface area and the root tip number of each group of plant roots after 45 days of treatment, and statistically analyzing the activities of SOD, CAT and POD of the roots, wherein the SOD refers to superoxide dismutase, the CAT refers to catalase, the POD refers to peroxidase and the ROS refers to plant active oxygen.
And S5, performing structural analysis according to the statistical data.
The analytical data are as follows:
the above-mentioned embodiments, objects, technical solutions and advantages of the present invention are further described in detail, it should be understood that the above-mentioned embodiments are only illustrative of the present invention and are not intended to limit the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A novel compound for improving the resistance of plants to soil aluminum toxicity is characterized in that: the product with melatonin and strigolactone as effective components is a new substance for improving the aluminum stress resistance of plants.
2. The method for preparing the novel compound for improving the resistance of plants to soil aluminum toxicity according to claim 1, wherein the method comprises the following steps: the effective components of the compound are melatonin and strigolactone mixed solution.
3. The method for preparing the novel compound for improving the resistance of plants to soil aluminum toxicity according to claim 1, wherein the method comprises the following steps: the concentration of the compound mixed solution is as follows: melatonin is 10-100 mu mol/L, and strigolactone is 0.1-1 mu mol/L.
4. The method for preparing the novel compound for improving the resistance of plants to soil aluminum toxicity according to claim 1, wherein the method comprises the following steps: the concentration of the compound aqueous solution is 10 mu mol/L melatonin and 1 mu mol/L strigolactone.
5. The method for preparing the novel compound for improving the resistance of plants to soil aluminum toxicity according to claim 1, wherein the method comprises the following steps: the compound aqueous solution is prepared by diluting a melatonin ethanol solution of 10mmol/L and a strigolactone methanol solution of 1 mmol/L.
6. The method for preparing the novel compound for improving the resistance of plants to soil aluminum toxicity according to claim 1, wherein the method comprises the following steps: the volume ratio of the melatonin to the strigolactones is 1-2: 1.
7. the method for preparing the novel compound for improving the resistance of plants to soil aluminum toxicity according to claim 1, wherein the method comprises the following steps: the volume ratio of the melatonin to the strigolactones is 1: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010748900.XA CN111802393A (en) | 2020-07-30 | 2020-07-30 | Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010748900.XA CN111802393A (en) | 2020-07-30 | 2020-07-30 | Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof |
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| CN111802393A true CN111802393A (en) | 2020-10-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010748900.XA Pending CN111802393A (en) | 2020-07-30 | 2020-07-30 | Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111826383A (en) * | 2020-07-16 | 2020-10-27 | 昆明理工大学 | Application of Danbo black soybean superoxide dismutase gene in improving plant aluminum tolerance |
| CN112673945A (en) * | 2020-12-02 | 2021-04-20 | 佛山科学技术学院 | Method for enhancing aluminum resistance of peas |
| CN115067101B (en) * | 2022-07-15 | 2023-08-18 | 青岛农业大学 | New application of strigolactone in improving potassium deficiency stress resistance of plants |
-
2020
- 2020-07-30 CN CN202010748900.XA patent/CN111802393A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 吕婷: "褪黑素对小麦铝毒的缓解作用及其机理研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 陈虞超等: "新型植物激素独角金内酯的研究进展", 《中国农学通报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111826383A (en) * | 2020-07-16 | 2020-10-27 | 昆明理工大学 | Application of Danbo black soybean superoxide dismutase gene in improving plant aluminum tolerance |
| CN111826383B (en) * | 2020-07-16 | 2022-08-02 | 昆明理工大学 | Application of Danbo black soybean superoxide dismutase gene in improving plant aluminum tolerance |
| CN112673945A (en) * | 2020-12-02 | 2021-04-20 | 佛山科学技术学院 | Method for enhancing aluminum resistance of peas |
| CN112673945B (en) * | 2020-12-02 | 2022-12-20 | 佛山科学技术学院 | Method for enhancing aluminum resistance of peas |
| CN115067101B (en) * | 2022-07-15 | 2023-08-18 | 青岛农业大学 | New application of strigolactone in improving potassium deficiency stress resistance of plants |
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