CN112673945A - Method for enhancing aluminum resistance of peas - Google Patents
Method for enhancing aluminum resistance of peas Download PDFInfo
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- CN112673945A CN112673945A CN202011390281.8A CN202011390281A CN112673945A CN 112673945 A CN112673945 A CN 112673945A CN 202011390281 A CN202011390281 A CN 202011390281A CN 112673945 A CN112673945 A CN 112673945A
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- 229910052782 aluminium Inorganic materials 0.000 title claims abstract description 33
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 8
- 235000010582 Pisum sativum Nutrition 0.000 title claims description 25
- 240000004713 Pisum sativum Species 0.000 title claims description 10
- 239000002253 acid Substances 0.000 claims abstract description 16
- 230000006978 adaptation Effects 0.000 claims abstract description 9
- 238000002791 soaking Methods 0.000 claims description 25
- 241000219843 Pisum Species 0.000 claims description 18
- 239000001110 calcium chloride Substances 0.000 claims description 17
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 17
- 230000035784 germination Effects 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 10
- 239000012535 impurity Substances 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 6
- 239000012498 ultrapure water Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000001133 acceleration Effects 0.000 claims description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 7
- 230000001988 toxicity Effects 0.000 abstract description 7
- 239000004411 aluminium Substances 0.000 abstract description 6
- 229920000018 Callose Polymers 0.000 abstract description 5
- 238000009825 accumulation Methods 0.000 abstract description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract description 5
- 230000007704 transition Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 8
- 239000003642 reactive oxygen metabolite Substances 0.000 description 6
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 210000000582 semen Anatomy 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 2
- YXOLAZRVSSWPPT-UHFFFAOYSA-N Morin Chemical compound OC1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 YXOLAZRVSSWPPT-UHFFFAOYSA-N 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 2
- 235000007708 morin Nutrition 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 1
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium chloride Substances Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for enhancing pea aluminum resistance, which comprises the step of applying GR24 solution with the concentration of 30-150 nmol/L to pea plants. Compared with the prior art, the GR24 is added in the low-acid adaptation treatment step, so that the elongation of the root system can be obviously promoted, and the aluminum accumulation in the root zone, especially the aluminum content in the root tip transition zone, can be effectively reduced. Reduce the root tip transition zone callose accumulation that leads to by the aluminium toxicity, effectively reduce the content of root tip ROS, reduce the cell programmed death speed that ROS leads to and promote root system to grow to reach the effect of alleviating the aluminium toxicity, improve the resistant aluminium nature of plant.
Description
Technical Field
The invention relates to the technical field of planting, in particular to a method for enhancing aluminum resistance of peas.
Background
Aluminum is the most abundant metal element in the crust and accounts for 7.45% of the total weight of the crust, wherein 50% of the acid arable land is poisoned by aluminum. The aluminum toxicity is a main inhibiting factor for the growth of plants in acid soil, and the aluminum can influence the growth of crop roots at the nanomolar concentration level, and is mainly characterized in that main roots become thick and short, root tips expand and brown, lateral roots and root hairs are reduced, the root tips are bent and irregularly hooked, root crowns fall off, and the biological oxidation capacity of the roots is obviously reduced. The aluminum toxicity severely limits the growth of crops, reduces the crop yield and has great influence on world grains.
Disclosure of Invention
The invention discloses a method for enhancing aluminum resistance of peas, which aims to solve one or more technical problems in the prior art and provide at least one beneficial choice or creation condition.
A method for enhancing aluminum resistance of peas comprises the step of applying GR24 solution with the concentration of 30-150 nmol/L to pea plants.
The specific method for enhancing the aluminum resistance of peas comprises the following steps:
1) seed soaking and germination accelerating: soaking peas in 5-10% NaClO for 30min for disinfection and germination acceleration, cleaning with sterile ultrapure water for several times at an interval of 5min, removing impurities, and adding 0.5-0.6 mmol/L CaCl2Soaking seeds in the solution for 10-12 h, and keeping the temperature at 24 +/-2 ℃ under the dark room condition;
2) aeroponics: putting the pea seeds which are fully swelled into an aeroponic box, and adding 0.5-1.0 mmol/L CaCl into the aeroponic box2Immersing the solution in an aeroponics device, and carrying out aeroponics for 48-72 h by using CaCl2The solution is replaced every 24 h; CaCl appears in the process2When the solution is not enough (the exposure of the pea seeds exceeds 1/3), the pea seeds need to be replenished and topped up;
3) water culture: after the pea seeds germinate, selecting seedlings with consistent root length and 3-5 cm high, preparing a Hoagland nutrient solution with the concentration of 1/4-1/2 as a culture solution A, and soaking the roots of the seedlings in the culture solution; and the culture solution A is soaked for more than 24 hours;
4) low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1-0.2 mmol/L CaCl20.3 to 0.6mmol/L KCl and 30 to 150nmol/L GR24, and the pH is 4.5.
Compared with the prior art, the GR24 is added in the low-acid adaptation treatment step, so that the elongation of the root system can be obviously promoted, and the aluminum accumulation in the root zone, especially the aluminum content in the root tip transition zone, can be effectively reduced. Reduce the root tip transition zone callose accumulation that leads to by the aluminium toxicity, effectively reduce the content of root tip ROS, reduce the cell programmed death speed that ROS leads to and promote root system to grow to reach the effect of alleviating the aluminium toxicity, improve the resistant aluminium nature of plant.
Drawings
FIG. 1 is a graph comparing the relative elongation of lateral roots after GR24 treatment at different concentrations as described in example 5;
FIG. 2 is a graph showing the comparison of the root tip hematoxylin staining of example 3 and comparative example 1;
FIG. 3 is a graph comparing the measurement of aluminum content in example 3 with that in comparative example 1;
FIG. 4 is a graph showing the comparison of the root tip Morin staining of example 3 and comparative example 1;
FIG. 5 is a graph comparing the amount of callose accumulated at the root tip in example 3 with that in comparative example 1;
FIG. 6 is a graph comparing the root tip ROS (reactive oxygen species) levels of example 3 and comparative example 1.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below.
Example 1
1) Seed soaking and germination accelerating: soaking semen Pisi Sativi in 7.5% NaClO for 30min for disinfection and germination, cleaning with sterile ultrapure water for 5 times, cleaning for 5min, removing impurities, and adding 0.5mmol/L CaCl2Soaking the seeds in the solution for 10 hours, and keeping the temperature at 24 ℃ under the dark room condition;
2) aeroponics: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5mmol/L CaCl into the aeroponic box2Immersing the solution in an atomizer, atomizing and culturing for 48h when CaCl is added2When the solution is not enough, it needs to be filled with CaCl2The solution is replaced every 24 h;
3) water culture: after the pea seeds germinate, selecting seedlings with consistent root length and height of 4 +/-0.1 cm, preparing a Hoagland nutrient solution with the concentration of 1/4 as a culture solution A, and soaking the roots of the seedlings in the culture solution; and the culture solution A is soaked for 24 hours;
4) low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1mmol/L CaCl20.5mmol/L KCl and 30nmol/L GR24, pH 4.5; the low acid is adapted for 10 h.
Example 2
1) Seed soaking and germination accelerating: soaking semen Pisi Sativi in 7.5% NaClO for 30min for disinfection and germination, cleaning with sterile ultrapure water for 5 times, cleaning for 5min, removing impurities, and adding 0.5mmol/L CaCl2Soaking the seeds in the solution for 10 hours, and keeping the temperature at 24 ℃ under the dark room condition;
2) aeroponics: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5mmol/L CaCl into the aeroponic box2Immersing the solution in an atomizer, atomizing and culturing for 48h when CaCl is added2When the solution is not enough, it needs to be filled with CaCl2The solution is replaced every 24 h;
3) water culture: after the pea seeds germinate, selecting seedlings with consistent root length and height of 4 +/-0.1 cm, preparing a Hoagland nutrient solution with the concentration of 1/4 as a culture solution A, and soaking the roots of the seedlings in the culture solution; and the culture solution A is soaked for 24 hours;
4) low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1mmol/L CaCl20.5mmol/L KCl and 60nmol/L GR24, pH 4.5; the low acid is adapted for 10 h.
Example 3
1) Seed soaking and germination accelerating: soaking semen Pisi Sativi in 7.5% NaClO for 30min for disinfection and germination, cleaning with sterile ultrapure water for 5 times, cleaning for 5min, removing impurities, and adding 0.5mmol/L CaCl2Soaking the seeds in the solution for 10 hours, and keeping the temperature at 24 ℃ under the dark room condition;
2) aeroponics: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5mmol/L CaCl into the aeroponic box2Immersing the solution in an atomizer, atomizing and culturing for 48h when CaCl is added2When the solution is not enough, it needs to be filled with CaCl2The solution is replaced every 24 h;
3) water culture: after the pea seeds germinate, selecting seedlings with consistent root length and height of 4 +/-0.1 cm, preparing a Hoagland nutrient solution with the concentration of 1/4 as a culture solution A, and soaking the roots of the seedlings in the culture solution; and the culture solution A is soaked for 24 hours;
4) low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1mmol/L CaCl20.5mmol/L KCl and125nmol/L GR24, pH 4.5; the low acid is adapted for 10 h.
Comparative example 1
1) Seed soaking and germination accelerating: soaking semen Pisi Sativi in 7.5% NaClO for 30min for disinfection and germination, cleaning with sterile ultrapure water for 5 times, cleaning for 5min, removing impurities, and adding 0.5mmol/L CaCl2Soaking the seeds in the solution for 10 hours, and keeping the temperature at 24 ℃ under the dark room condition;
2) aeroponics: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5mmol/L CaCl into the aeroponic box2Immersing the solution in an atomizer, atomizing and culturing for 48h when CaCl is added2When the solution is not enough, it needs to be filled with CaCl2The solution is replaced every 24 h;
3) water culture: after the pea seeds germinate, selecting seedlings with consistent root length and height of 4 +/-0.1 cm, preparing a Hoagland nutrient solution with the concentration of 1/4 as a culture solution A, and soaking the roots of the seedlings in the culture solution; and the culture solution A is soaked for 24 hours;
4) low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1mmol/L CaCl20.5mmol/L KCl, pH 4.5; the low acid is adapted for 10 h.
Example 5
The aluminum-resistant treated pea seedlings obtained in examples 1 to 3 and comparative example 1 were subjected to aluminum treatment. So as to contain 0.1mmol/L CaCl2，0.5mmol/L KCl，15μmol/L AlCl3Soaking the culture solution for 24h, and then respectively carrying out lateral root length measurement, root tip hematoxylin staining, aluminum content measurement, root tip Morin staining, root tip callose accumulation amount observation and root tip ROS (reactive oxygen species) level measurement. The results are shown in FIGS. 1 to 6. GR24 at various concentrations all served to enhance the aluminum resistance of peas, but the preferred value was 125 nmol/L. The subsequent comparisons were therefore made with example 3 as the "+ GR 24" sample and with comparative example 1 as the "-GR 24" sample.
The GR24 with different concentrations is adopted in embodiments 1-3 to carry out low-acid adaptive treatment, and comparison shows that the pretreatment with GR24 can effectively reduce the content of aluminum in the root tip, simultaneously reduce the generation of callose and active oxygen in the root tip under the stress of aluminum, and relieve the inhibition of aluminum on the elongation of the root, thereby achieving the effect of relieving aluminum toxicity and improving the aluminum resistance of plants.
Claims (9)
1. A method for enhancing the aluminum resistance of peas is characterized in that GR24 solution with the concentration of 30-150 nmol/L is applied to pea plants.
2. The method of claim 1, comprising the steps of:
1) seed soaking and germination accelerating;
2) carrying out aeroponic culture;
3) water culture;
4) low-acid adaptation treatment;
the low-acid adaptation treatment is to soak the root system of the pea plant in GR24 solution with the concentration of 30-150 nmol/L for at least 24 h.
3. The method of claim 1, wherein step 1) comprises: soaking peas in 5-10% NaClO for 10-60 min for disinfection and germination acceleration, then cleaning with sterile ultrapure water for several times, removing impurities, and adding 0.5-0.6 mmol/L CaCl2Soaking the seeds in the solution for 10-12 h, and keeping the temperature at 24 +/-2 ℃ under the dark room condition.
4. The method of claim 1, wherein step 2) comprises: putting the pea seeds which are fully swelled into an aeroponic box, and adding 0.5-1.0 mmol/L CaCl into the aeroponic box2Immersing the solution in an aeroponics device, and carrying out aeroponics culture for 48-72 h.
5. The method of claim 4, wherein the CaCl of step 2) is2The solution was changed every 24 h.
6. The method of claim 1, wherein step 3) comprises: preparing a Hoagland nutrient solution with the concentration of 1/4-1/2 as a culture solution A for the seedlings after pea seeds sprout, and soaking the roots of the seedlings with the culture solution.
7. The method as claimed in claim 5, wherein the seedlings in step 3) are required to have consistent root length and height of 3-5 cm.
8. The method of claim 5, wherein step 4) comprises: changing the culture solution A into a culture solution B; the culture solution B contains 0.1-0.2 mmol/L CaCl20.3 to 0.6mmol/L KCl and 30 to 150nmol/L GR24, and the pH is 4.5.
9. The method according to claim 8, wherein the conditions for replacing culture solution A with culture solution B in step 4) are as follows: the length of the maximum lateral root of the seedling reaches 1-2 cm.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010128112A2 (en) * | 2009-05-07 | 2010-11-11 | Gmi - Gregor-Mendel-Institut Für Molekulare Pflanzenbiologie Gmbh | Use of strigolactones |
| CN104542587A (en) * | 2014-12-18 | 2015-04-29 | 昆明理工大学 | Application of chlorogenic acid in relieving of aluminum toxicity in soybean seedlings |
| CN108040844A (en) * | 2017-11-15 | 2018-05-18 | 佛山科学技术学院 | A kind of aluminum-resistant poison pea seedling inoculation method |
| CN111802393A (en) * | 2020-07-30 | 2020-10-23 | 青岛农业大学 | Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof |
-
2020
- 2020-12-02 CN CN202011390281.8A patent/CN112673945B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010128112A2 (en) * | 2009-05-07 | 2010-11-11 | Gmi - Gregor-Mendel-Institut Für Molekulare Pflanzenbiologie Gmbh | Use of strigolactones |
| CN104542587A (en) * | 2014-12-18 | 2015-04-29 | 昆明理工大学 | Application of chlorogenic acid in relieving of aluminum toxicity in soybean seedlings |
| CN108040844A (en) * | 2017-11-15 | 2018-05-18 | 佛山科学技术学院 | A kind of aluminum-resistant poison pea seedling inoculation method |
| CN111802393A (en) * | 2020-07-30 | 2020-10-23 | 青岛农业大学 | Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof |
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