CN109392583A - A method of promote peach embryo to train rosette-stape seedling seedling - Google Patents
A method of promote peach embryo to train rosette-stape seedling seedling Download PDFInfo
- Publication number
- CN109392583A CN109392583A CN201811500859.3A CN201811500859A CN109392583A CN 109392583 A CN109392583 A CN 109392583A CN 201811500859 A CN201811500859 A CN 201811500859A CN 109392583 A CN109392583 A CN 109392583A
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- Prior art keywords
- seedling
- stape
- rosette
- peach
- embryo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a kind of methods of promotion peach embryo training rosette-stape seedling seedling, and its step are as follows: after peach seeds are sterile-processed, being inoculated into container；Container, which is placed into Cool Room 4 DEG C, carries out low-temperature treatment, to break seed dormancy；When seed base-root is sprouted, illumination cultivation is carried out in culturing room；Embryo is trained seedling and is transplanted after a period of time is cultivated by culturing room；Matrix is installed in hole tray, is sprinkled profoundly water, and embryo training seedling washes root culture medium and plants into hole tray；By the growing point of GA3 point to rosette-stape seedling, normal condition culture.And it discloses GA3 and is promoting the application in peach embryo training rosette-stape seedling seedling.The beneficial effects of the present invention are: peach embryo training rosette-stape seedling is handled using GA3, can promote the pumping young sprout of rosette-stape seedling, growing up to has the seedling for continuing culture value.Number is it was demonstrated that the GA concentration pumping young sprout quantity of 1mg/ml is more, and new-tip length is shorter, field run plant rate highest.
Description
Technical field
The present invention relates to a kind of methods of promotion peach embryo training rosette-stape seedling seedling, belong to peach seedling raising technology field.
Background technique
Chilling requirement refers to break deciduous fruit tree nature dormancy needed for effective low temperature when number, or perhaps natural dormancy process
In effectively low temperature accumulation quantizating index.The height of chilling requirement is mainly determined by hereditary capacity, thus different tree species, kind
Between have differences.
In the torrid zone, subtropical zone warm winter area and protecting field cultivation of fruit tree, often because low temperature chilling requirement is not able to satisfy fruit tree
It needs, and causes rudiment, blooms irregular, or even if fruit development of having bloomed is bad, shedding, malformed fruit phenomenon occur.
Only by effective breaking dormancy, it is just able to achieve the normal growth of fruit.
Peach seeds are as the seed of other deciduous fruit trees, and there are dormancies.Peach seeds only pass through certain time
Ability breaking dormancy after cold stratification, sprouts into field run plant.When the low-temperature treatment time is inadequate, the In vitro Embryo of resting seed
It is slow to germinate, even if the buds are coming out, the state of dwarfed plant (physiology dwarfism) can be also presented in seedling, and stem is short and small, leaf rolling, abnormal
Shape, top are grown in rosette-stape.The seed of same kind, under the conditions of same treatment, individual also will appear difference, and part seedling is just
It is frequently grown, rosette-stape is presented in part.If being unable to pumping young leaves, the later period is more difficult normally to be grown, and planting percent is caused to decline.
Gibberellin (Gibberellins or gibberellic acid, GA) is that plant necessary to plant growth and development swashs
One of element, participates in development of plants and various physiology courses, and the elongation of growth, stem including root, vane extension, seed are sprouted, spent
With the development of fruit etc..The document that can be consulted at present, the processing before mostly being sprouted about gibberellin to seed, and about seed sprouting
The lotus throne state this respect for breaking seedling afterwards has not been reported.
Summary of the invention
It is an object of the present invention to provide a kind of methods of promotion peach embryo training rosette-stape seedling seedling
For the above-mentioned target of realization, technical solution of the present invention specific steps include:
(1) it after peach seeds are sterile-processed, is inoculated into container, container generally uses test tube；
(2) container, which is placed into Cool Room 4 DEG C, carries out low-temperature treatment, to break seed dormancy；
(3) when seed base-root is sprouted, illumination cultivation is carried out in culturing room；
(4) embryo training seedling is transplanted after a period of time is cultivated by culturing room；
(5) matrix is installed in hole tray, sprinkled profoundly water, embryo training seedling washes root culture medium and plants into hole tray；
(6) growing point of GA3 point to rosette-stape seedling can be kept GA3 to retain in growing point by selection rainy weather in this way
Time at 30 minutes or more, normal condition culture.
Preferably, radicle sprouts length for 0.5-1.5cm, if continuing to be put into freezer culture, leaf bud is given birth in the dark
It is long, cause hypocotyl too long, plant strain growth gesture is weak, and when seedling is top-heavy, easily lodges, makes troubles to final-period management.
Preferably, step (4) culture is to grow 5 young leaves for a period of time, and root is flourishing, subject to 5 or more.Probably need
Want 3 weeks or more time.
Preferably, the concentration of the GA3 is 1-8mg/ml.
Preferably, culture room temperature is set as 24 DEG C.
The invention also discloses GA3 to promote the application in peach embryo training rosette-stape seedling seedling.
The beneficial effects of the present invention are: peach embryo training rosette-stape seedling is handled using GA3, can promote rosette-stape seedling again
Pumping goes out young sprout, and then forms normal stem section, and growing up to has the seedling for continuing culture value.Number is it was demonstrated that the GA3 of 1mg/ml is dense
Degree pumping young sprout quantity is more, and new-tip length is shorter, field run plant rate highest.
Specific embodiment
Embodiment 1
Promote the method for rosette-stape peach seedling seedling, step includes:
(1) it after precocious peach seeds are sterile-processed, is inoculated into test tube；
(2) test tube, which is placed into Cool Room 4 DEG C, carries out low-temperature treatment, to break seed dormancy；
(3) when seed base-root is sprouted, illumination cultivation is carried out in 24 ° of culturing room；
(4) embryo training seedling was transplanted at culturing room's culture 3 weeks or more；
(5) matrix is installed in hole tray, waters water, being shaken hands with soil, inner agglomerating release is i.e. scattered (to impregnate because of the too many root system of water
To in water, very little moisture is inadequate) subject to, embryo training seedling washes root culture medium and plants into hole tray, and watering can rinses seedling well.
(6) hole tray covers moisturizing cover, and that there are the holes of 4 diameter 1.5cm is ventilative, and temperature control is at 22-28 DEG C, temperature
Too it is low can extend seedling-slowing stage it is unsuitable raw new root system, slow growth, too high easy burn seedlings.
(7) GA3 concentration is set as tetra- kinds of various concentrations of 1mg/ml, 2mg/ml, 4mg/ml, 8mg/ml, respectively with cotton balls point
Rosette-stape seedling growing point is compareed without any processing；Each processing growing state is investigated after one month, the results are shown in Table 1.From
Table 1 as can be seen that the GA concentration of 1mg/ml pumping young sprout quantity is more again, new-tip length is shorter, field run plant rate highest.
Condition survey after table 1GA processing
Claims (6)
1. a kind of method for promoting peach embryo training rosette-stape seedling seedling, its step are as follows:
(1) it after peach seeds are sterile-processed, is inoculated into container；
(2) container, which is placed into Cool Room 4 DEG C, carries out low-temperature treatment, to break seed dormancy；
(3) when seed base-root is sprouted, illumination cultivation is carried out in culturing room；
(4) embryo training seedling is transplanted after a period of time is cultivated by culturing room；
(5) matrix is installed in hole tray, sprinkled profoundly water, embryo training seedling washes root culture medium and plants into hole tray；
(6) the rosette-stape seedling being transplanted in hole tray, with its growing point of GA3 point, normal condition culture.
2. the method according to claim 1 for promoting peach embryo training rosette-stape seedling seedling, it is characterised in that: embryo in step (3)
Root sprouts length for 0.5-1.5cm.
3. the method according to claim 1 for promoting peach embryo training rosette-stape seedling seedling, it is characterised in that: step (4) culture
It is to grow 5 young leaves for a period of time, root is flourishing, subject to 5 or more.
4. the method according to claim 1 for promoting peach embryo training rosette-stape seedling seedling, it is characterised in that: the GA3's is dense
Degree is 1mg/ml.
5. the method according to claim 2 for promoting peach embryo training rosette-stape seedling seedling, it is characterised in that: culture room temperature is set
It is set to 24 DEG C.
6.GA3 is promoting the application in peach embryo training rosette-stape seedling seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811500859.3A CN109392583A (en) | 2018-12-10 | 2018-12-10 | A method of promote peach embryo to train rosette-stape seedling seedling |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811500859.3A CN109392583A (en) | 2018-12-10 | 2018-12-10 | A method of promote peach embryo to train rosette-stape seedling seedling |
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| Publication Number | Publication Date |
|---|---|
| CN109392583A true CN109392583A (en) | 2019-03-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811500859.3A Pending CN109392583A (en) | 2018-12-10 | 2018-12-10 | A method of promote peach embryo to train rosette-stape seedling seedling |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111487291A (en) * | 2020-04-24 | 2020-08-04 | 江苏省农业科学院 | Method for efficiently evaluating cooling capacity required by peach blossom buds based on electronic nose detection technology |
| CN112314308A (en) * | 2020-11-10 | 2021-02-05 | 上海市农业科学院 | Method for remedying seedling with blight in peach embryo culture process |
Citations (3)
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| CN105104195A (en) * | 2015-08-22 | 2015-12-02 | 中国农业科学院郑州果树研究所 | Technology for obtaining peach embryo aseptic seedlings on large scale |
| CN105815188A (en) * | 2016-05-30 | 2016-08-03 | 中国农业科学院郑州果树研究所 | Mid-late maturity peach seed direct germinating and seedling culturing technology |
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Patent Citations (3)
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| CN105104195A (en) * | 2015-08-22 | 2015-12-02 | 中国农业科学院郑州果树研究所 | Technology for obtaining peach embryo aseptic seedlings on large scale |
| CN105815188A (en) * | 2016-05-30 | 2016-08-03 | 中国农业科学院郑州果树研究所 | Mid-late maturity peach seed direct germinating and seedling culturing technology |
| CN107750948A (en) * | 2017-10-26 | 2018-03-06 | 江苏省农业科学院 | The method for improving peach hybridization planting percent |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111487291A (en) * | 2020-04-24 | 2020-08-04 | 江苏省农业科学院 | Method for efficiently evaluating cooling capacity required by peach blossom buds based on electronic nose detection technology |
| CN111487291B (en) * | 2020-04-24 | 2022-11-29 | 江苏省农业科学院 | Method for efficiently evaluating cooling capacity required by peach blossom buds based on electronic nose detection technology |
| CN112314308A (en) * | 2020-11-10 | 2021-02-05 | 上海市农业科学院 | Method for remedying seedling with blight in peach embryo culture process |
| CN112314308B (en) * | 2020-11-10 | 2022-04-01 | 上海市农业科学院 | Method for remedying seedling with blight in peach embryo culture process |
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