KR102565522B1 - Method for mass propagation of high quality seedlings of Myrica rubra - Google Patents

Abstract

In the present invention, a method for mass-producing excellent seedlings of blackberry tree in which the seed germination rate and the long-term stable growth rate of seedlings are significantly improved through a dry heat pretreatment process under specific conditions and a gibberellin treatment process under specific conditions is disclosed.
According to the method for mass production of good seedlings of blackberry according to the present invention, the germination rate of blackberry seeds is increased while the root elongation is improved, and the long-term stable growth rate of germinated blackberry seedlings is remarkably promoted. It is possible to mass-produce excellent seedlings of the blackberry tree that are resistant to stress such as overcrowding and excessive fertilization. In addition, since the excellent seedlings of the blackberry tree according to the present invention are healthy seedlings with good long-term growth, they can be used for mass afforestation.

Description

Method for mass propagation of high quality seedlings of Myrica rubra}

The present invention relates to a method for mass-producing excellent seedlings of blackberry tree, and more particularly, the seed germination rate and long-term stable growth rate of seedlings are remarkably improved through a dry heat pre-treatment process and a gibberellin treatment process under specific conditions of blackberry seedlings under specific conditions. It relates to a method for mass-producing excellent seedlings of blackberry.

It is a rare plant native to Jeju Island and is known to be distributed in China, Taiwan, Japan and the Philippines. It is used for food, medicine, and dyes, and is also used as a new carbon material, garden tree, roadside tree, park tree, and ornamental tree. The fruit is effective for diarrhea, digestion improvement, thirst quenching, stomatitis, and bruises, the bark is used as a blood pressure lowering agent or diuretic, and the leaves are used as an antidiarrheal. In recent years, cosmetics have been developed, and improved species are cultivated called Yangmae and Red Bayberry. In addition, it has been found that blackberry is effective in hair loss, prevention of skin aging, and treatment of mite allergens (Registration Patent No. 10-0872454. Patent Publication No. 10-2008-0078753).

As such, the blackberry tree has high conservation value as a rare plant, as well as utilization value as a natural material and ornamental value. However, research on the breeding of the blackberry tree is not sufficiently conducted domestically and internationally. In particular, the blackberry tree has a low seed germination rate and long-term after seed germination Stable growth was not guaranteed, so it was difficult to mass-produce excellent seedlings.

The promotion of plant seed germination rate and long-term stable growth rate of seedlings is very important in plant cultivation. As seed treatment to increase the germination rate of seeds, priming treatment, hormone treatment including gibberellin, light treatment, etc. have been used (Registration Patent No. 10-1412052), but a method for simultaneously enhancing the long-term stable growth rate of seedlings has been developed. there is no bar In particular, in the case of blackberry, there has been no report so far about a technology that simultaneously improves seed germination rate and long-term stable growth rate of seedlings.

Therefore, it is urgently required to develop a method for mass-producing excellent seedlings of blackberry by simultaneously increasing the seed germination rate and long-term stable growth rate of seedlings.

Accordingly, the present inventors have continuously studied to meet the needs of the prior art, and as a result, when a dry heat pretreatment process under specific conditions and a gibberellin treatment process under specific conditions are combined, the seed germination rate and seedling long-term The present invention was completed by confirming that the stable growth rate was remarkably improved to mass-produce excellent seedlings of the Japanese apricot tree.

Accordingly, an object of the present invention is to provide a method for mass-producing excellent seedlings of the Japanese apricot tree.

Another object of the present invention is to provide superior seedlings of the blackberry produced by the above method.

In order to achieve the above object of the present invention, the present invention

i) sterilizing blackberry seeds;

ii) dry heat treatment of the sterilized seeds at 35 to 45° C. for 15 to 55 hours;

iii) immersing the dry heat-treated seeds in an aqueous solution of gibberellin at a concentration of 500 to 1,500 ppm for 20 to 24 hours;

iv) sowing and germinating the blackberry seed from step iii); and

v) growing the germinated seedlings to obtain excellent seedlings of the blackberry tree;

Step i) Seed sterilization

Sterilize the seeds of the blackberry tree.

In the present invention, sterilization may be performed by immersing and washing blackberry seeds in water for 3 to 5 days, treating them with sodium hypochlorite (NaOCl) solution for 5 to 10 minutes, and then washing with water. Preferably, after immersing the blackberry seeds in running water for 5 days, treating them with 1% sodium hypochlorite for 10 minutes, washing them three times in running water, and then washing them once with distilled water to sterilize the blackberry seeds.

According to this step, it is possible to remove microorganisms and germination inhibitors that can cause decay present on the surface of the seeds without reducing the germination rate when sterilizing the seeds. In addition, germination can be promoted by softening the tissue.

step ii) dry heat treatment

Sterilized seeds are treated with dry heat at 35-45 ° C for 15-55 hours.

In this step, 'dry heat treatment' means heat treatment using dry heat, not immersion using hot water, and preferably, dry heat treatment can be performed using a dry heat machine or dry oven, but is not limited thereto.

The dry heat treatment temperature is preferably maintained at a constant temperature in the range of 35 ~ 45 ℃, if the temperature is less than 35 ℃, the heat treatment effect may be insufficient, if it exceeds 45 ℃, the seeds may deteriorate. Most preferably, dry heat treatment is performed at 40°C.

The dry heat treatment time is preferably 15 to 55 hours. If it is less than 15 hours, the heat treatment effect may be insufficient, and if it exceeds 55 hours, the seeds may deteriorate.

According to this step, the long-term stable growth rate of seedlings is significantly improved without deterioration of seed germination rate when dry heat treatment of blackberry seeds (FIG. 7a, 7b, Table 2).

Step iii) Gibberellin Treatment

Dry heat-treated seeds are immersed in an aqueous solution of gibberellin at a concentration of 500 to 1,500 ppm for 20 to 24 hours.

Gibberellins (GAs) are plant hormones that regulate plant growth, including stem elongation, germination, dormancy, flowering and growth of flowers, and senescence of leaves and fruits.

The concentration of gibberellin in this step is preferably 500 to 1,500 ppm, most preferably 1,000 ppm.

If the concentration of gibberellin is less than 500 ppm, the germination rate of blackberry seeds may not be properly improved, and if it exceeds 1,500 ppm, it may rather cause germination inhibition.

The treatment time of gibberellin is preferably 20 to 24 hours, and if the treatment time of gibberellin is less than 20 hours, the gibberellin may not be sufficiently penetrated into the seeds.

In the case of only gibberellin treatment without dry heat treatment in step ii), the germination rate of blackberry seeds is improved, but there is a problem in that the long-term stable growth rate of seedlings is lowered (FIG. 7a, FIG. 7b, Table 2).

After the dry heat treatment in step ii), when the gibberellin treatment is performed according to this step, the germination rate of blackberry seeds is further enhanced, and the long-term stable growth rate of seedlings can be maintained high (FIG. 7a, FIG. 7b, Table 2).

Step iv) Germination

Sow and germinate the blackberry seeds from step iii).

In this step, seeding is preferably performed in horticultural soil at a temperature of 12 to 27 ° C. Outside the above temperature range, germination may be inhibited.

When spot sowing, the spacing is preferably 5 cm or more. If this range is not reached, germination and growth may be hindered, such as tangled roots, overlapping leaves, and lack of nutrients while the seedlings are growing.

In the case of high temperature and high humidity and low water pressure, it is desirable not to fertilize or to use a small amount.

Sowing is usually carried out under dark conditions by covering the soil to retain moisture in the seeds.

Germination in this step is preferably carried out under light-shielding conditions.

Stage v) Growth

Germinated seedlings are grown to obtain excellent seedlings of the sagebrush.

Growth in this step can be carried out by growing for 10 to 24 months after sowing at a temperature of 0.5 to 43.5 ° C. under shade conditions.

If the growth temperature is less than 0.5 ° C, the growth of the seedlings may not be sufficiently achieved, and if the temperature is higher than 43.5 ° C, the growth of the seedlings may be inhibited due to the high temperature.

Preferably, 6-8 months after sowing, the seedlings are transplanted into new soil at intervals of 15-25 cm and grown. At this time, the seedling height is 2 cm or more, the seedling diameter is 0.7 mm or more, and the number of leaves is preferably 4 or more. If this number is not reached, the possibility of growth after transplantation is low.

According to the production method of the present invention, the germination rate at 210 days after sowing is 78% or more, and the growth rate at 306 days after sowing is 66% or more.

According to the production method of the present invention, by using a combination of dry heat treatment and gibberellin treatment, the seed germination rate of the blackberry tree is further improved, the root elongation is good, and the long-term stable growth rate of the blackberry seedlings is remarkably improved. It is possible to mass-produce excellent seedlings of

In accordance with another object of the present invention, the present invention provides superior seedlings of Japanese sagebrush produced by the above production method.

The excellent seedlings of the blackberry tree according to the present invention are excellent seedlings that can grow stably for a long period of time and are suitable for mass afforestation.

The excellent seedlings of the blackberry tree according to the present invention have a germination rate of 78% or more at 210 days after seed sowing, and a growth rate of 66% or more at 306 days after sowing.

According to the method for mass production of good seedlings of blackberry according to the present invention, the germination rate of blackberry seeds is increased while the root elongation is also improved, and the long-term stable growth rate of germinated blackberry seedlings is remarkably promoted. It is possible to mass-produce excellent seedlings of the blackberry tree that are resistant to stress such as overcrowding and excessive fertilization.

In addition, since the excellent seedlings of the blackberry tree according to the present invention are healthy seedlings with good long-term growth, they can be used for mass afforestation.

Figure 1 is a photograph of the mother plant from which seeds were collected.
Figure 2 is a photograph of the harvested barberry fruit (including seeds).
Figure 3 is a photograph of the process of dry heat treatment of blackberry seeds.
Figure 4 is a photograph of the process of treating blackberry seeds with gibberellin and distilled water.
Figure 5 is a photograph of the sogwi tree seeds used during sowing.
Figure 6 is a photograph of the seedlings of sogwi tree germinated after sowing.
Figure 7a is a graph showing the germination rate (210 days) of the seeds of sogwi tree according to the seed treatment.
Figure 7b is a graph showing the change in the survival rate of seedlings of sagebrush according to seed treatment.
Figure 8 is a photograph showing the root state before transplantation of seedlings of sogwi tree.
Figure 9 is a photograph showing the growth state of the transplanted sogwi tree.

Hereinafter, the configuration and effects of the present invention will be described in more detail through specific examples to aid understanding of the present invention. However, the following Examples and Experimental Examples are merely exemplified in order to more clearly understand the present invention, and the scope of the present invention is not limited by the following Examples.

Preparation Example 1: Seed sterilization of blackberry

Fruits were collected on June 17, 2019 from a colony of blackberry in Seogwipo, Jeju-do, the pulp was removed, the seeds were obtained, and the seeds were preserved at 4 ℃ for 100 days. It was prepared by treating for a minute, washing with running water three times, and then washing once with distilled water to complete sterilization (FIGS. 1 and 2).

Experimental Example 1: Analysis of improvement of germination rate of barberry tree

As shown in Table 1 below, the sterilized blackberry seeds were divided into 3 groups (Comparative Example 1, Comparative Example 2 and Example 1) of 100 each, and the seeds of Comparative Example 1 were soaked in 1,000 ppm gibberellin aqueous solution for 20 hours and 30 minutes. Only soaking treatment was performed, and the seeds of Comparative Example 2 were immersed in distilled water for 20 hours and 30 minutes after dry heat treatment in a dry oven at 40 ° C for 54 hours and 50 minutes, and the seeds of Example 1 were subjected to dry heat treatment in a dry oven at 40 ° C for 54 hours and 50 minutes. After the treatment, immersion treatment was performed in 1,000 ppm gibberellin aqueous solution for 20 hours and 30 minutes (FIG. 3, FIG. 4, FIG. 5).

seed count dry heat treatment gibberellin treatment Comparative Example 1 100 × ○ Comparative Example 2 100 ○ × Example 1 100 ○ ○

The sorghum seeds treated as described above were sown and germinated on October 11, 2019 on horticultural medium in a shaded greenhouse, and the germination rate of each group was calculated at 210 days after sowing and shown in FIG. 7a.

As shown in FIG. 7a, the germination rate of grapefruit seeds of Comparative Example 1 (gibberellin treatment group) was 74%, and 56% of the seeds of grapefruit seed of Comparative Example 2 (dry heat treatment group). According to these results, it can be confirmed that the gibberellin treatment effectively enhances the germination rate of grapefruit seeds, and the dry heat treatment lacks the effect of increasing the germination percentage of grapevine seeds.

In contrast, the germination rate of the genus chinensis seeds of Example 1 (dry heat treatment + gibberellin treatment) was 78%, and it could be confirmed that it was further enhanced compared to Comparative Example 1 (gibberellin treatment group).

In addition, the root elongation of the blackberry seedlings of Example 1 was also good (FIG. 8).

Experimental Example 2: Production of excellent seedlings of barberry

The germinated barberry seedlings of Comparative Example 1, Comparative Example 2, and Example 1 in Experimental Example 1 were transplanted to shaded field soil on the 237th day after sowing (June 3, 2020) and continued to grow. After sowing, 237 The growth rates of each group were calculated on days 270 and 306, and are shown in Table 2 and Figure 7b.

Growth rate (%) 210 days 237 days 270 days 306 days Comparative Example 1 74 32 30 29 Comparative Example 2 56 52 47 46 Example 1 78 66 66 66

As shown in Table 2 and FIG. 7B, in the case of Example 1 treated with gibberellin after dry heat treatment according to the present invention, the survival rate of the seedlings of sagebrush was 66% at 237 days, 66% at 270 days, and 66% at 306 days. After transplantation, it can be confirmed that all of the seedlings of the barberry tree are not withered at all, and the high growth rate is stably maintained for a long period of time.

In contrast, in the case of the grape seedlings of Comparative Example 1 treated only with gibberellin, the survival rate was 32% at 237 days, 30% at 270 days, and 29% at 306 days, and the survival rate dropped sharply at 237 days and continued to survive thereafter. This decline can be seen.

In addition, in the case of the blackberry seedlings of Comparative Example 2 treated only with dry heat, the survival rate was 52% on the 237th day, 47% on the 270th day, and 46% on the 306th day, confirming that the survival rate continuously declined.

Comparing the long-term survival rate of blackberry seedlings at 306 days after sowing, Example 1 was 66%, which was about 2.3 times and 1.5 times higher than Comparative Example 1 and Fertilizer System 2 (29% and 46%, respectively). can

From the above results, when the combination of dry heat treatment and gibberellin treatment is used according to the present invention, the germination rate of cowberry seeds is improved, the root elongation is also improved, and the long-term stable growth rate of cowberry seedlings can be significantly improved. It can be seen that excellent seedlings of can be mass-produced.

Claims (11)

A method for mass-producing excellent seedlings of a blackberry tree, the method
i) sterilizing blackberry seeds;
ii) dry heat treatment of the sterilized seeds at 40 to 45° C. for 15 to 55 hours;
iii) immersing the dry heat-treated seeds in an aqueous solution of gibberellin at a concentration of 500 to 1,500 ppm for 20 to 24 hours;
iv) sowing and germinating the blackberry seed from step iii) at a temperature of 12 to 27° C. and shade conditions; and
v) growing the germinated seedlings under shaded conditions at a temperature of 0.5 to 43.5 ° C. for 10 to 24 months to obtain excellent seedlings of barberry trees;
The dry heat treatment in step ii) is performed using a dry heater or a dry oven,
A method characterized in that the growth rate is 66% or more on the 306th day after sowing of the excellent seedlings of the sorrel tree.
delete delete The method for mass-producing superior seedlings of a blackberry tree according to claim 1, wherein the dry heat treatment in step ii) is performed at 40° C. for 54 hours and 50 minutes.
The method of claim 1, wherein the concentration of gibberellin in step iii) is 1,000 ppm.
delete delete The method for mass-producing excellent seedlings of a blackberry tree according to claim 1, wherein the seedlings are transplanted to new soil 6 to 8 months after sowing in step v) and grown.
delete delete delete