CN105165608A - Efficient rooting method of blueberry tissue culture seedlings - Google Patents
Efficient rooting method of blueberry tissue culture seedlings Download PDFInfo
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- CN105165608A CN105165608A CN201510554152.0A CN201510554152A CN105165608A CN 105165608 A CN105165608 A CN 105165608A CN 201510554152 A CN201510554152 A CN 201510554152A CN 105165608 A CN105165608 A CN 105165608A
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Abstract
The present invention belongs to the field of blueberry farming, and particularly relates to an efficient rooting method of blueberry tissue culture seedlings, wherein the method comprises strong seedling culture and rooting culture. According to the present invention, the rooting pre-treatment is used and VB1 and boric acid are added so as to enhance the root quality and the resistance of the plant to a certain extent and reduce the cultivate links, the cottage survival rate of the treated blueberry tissue culture seedling is significantly improved, the culture period is shortened, the production cost is reduced, and the large-scale production is easily achieved; when the cultivation medium is the mixture of peat and perlite according to a ratio of 1:1, the survival rate of the blueberry seedling is the highest, the plant height, the aboveground part biomass and the underground part biomass are highest, and the basal diameter and the number of the leaves are relatively high; and the new incubator-foam box is used to carry out field planting on the blueberry seedling, such that the culture blueberry seedling has characteristics of good growth, stout plant, developed root, more fibrous roots, and high survival rate.
Description
Technical field
The invention belongs to blueberry Field of Planting, be specifically related to a kind of high-effective root-growing method of blueberry tissue culture seedling.
Background technology
Blueberry also known as blueberry, blue berry, Ericaceae Vacciniodeae Vaccinium shrub berry fruit tree.Be rich in the compositions such as anthocyan, anthocyanin, catechuic acid, ursin, vitamin, have anti-inflammatory, anticancer, anti-aging, reducing blood lipid, norcholesterol, antibacterial, anti-osteoporosis, hypoglycemic, improve the effect such as memory, anti-vision degeneration.Because its peculiar flavour and good nutrition health-care functions are subject to the favor of people day by day, the international food and agricultural organization of Xian Yibei is classified as one of five large healthy food, and the demand in blueberry fresh fruit and converted products thereof at home and abroad market is increasing, and the development prospect of blueberry industry is very tempting.In the last few years, the cultivation development of blueberry more and more obtained the attention of countries in the world.The eighties, the states such as Japan, Holland, Germany, New Zealand introduce a fine variety blueberry from the U.S. and carry out batch production.The nineties, China also introduces excellent blueberry kind from the U.S., Canada in succession, and the breeding of blueberry improved seeds and efficiency cultivation technology for high have become study hotspot in recent years.
At present, the mating system of blueberry seedling has two kinds, i.e. green wood cutting breeding and tissue culture.Scale affects by cutting quantity, cutting plantation is low, reproduction speed is slow in green wood cutting breeding, cannot meet need of production in a short time, and the nursery stock bred also has weak point in all many-sides such as growth potential, fruit quality; And breed in the process of blueberry seedling at tissue culture technique, there is the problems such as difficulty, rooting rate are not high, rooting rate is slow of taking root again.The basis of blueberry tissue culture fast breeding technique maturation is carried out the technical research of blueberry subculture bud outside sprout-cultivating-bottle radication, cultivate link to reduce plantlet in vitro, shorten growing-seedling period, reduce seedling cost, improve blueberry tissue culture numerous and industrial breeding technique system soon further, applying of plantlet in vitro of promotion is had important practical significance.
Summary of the invention
For solving above technology Problems existing, the invention provides a kind of high-effective root-growing method of blueberry tissue culture seedling, the survival rate of plantlet in vitro can be improved, shorten the cultivation time, reduce production cost, adapt to suitability for industrialized production.
Its technical scheme is:
A high-effective root-growing method for blueberry tissue culture seedling, carry out according to following steps:
(1) strong seedling culture:
A. after choosing the shoot tip meristem segment of blueberry plant, sterilization, be seeded in and sprout in having in the bottle of medium to cultivate, cultivation temperature is 25 ± 2 DEG C, relative moisture > 95%, double-deck shade net is utilized to shelter from heat or light, place and cultivate about 30d, note observing plant elasticity, the change of stem stalk;
B. choose the bottle seedling grown fine, height, at more than 5cm, connects bottle and moves to green house, carry out strong seedling culture, cultivation temperature is 25 ± 2 DEG C, relative moisture > 95%, cultivate under illumination 2500-3000LX, light application time is 10-12h/d, places and cultivates about 30d;
(2) culture of rootage:
A. make the bubble chamber that length, width and height are respectively 54cm, 40cm, 25cm, bubble chamber bear, the diameter in hole is 2cm, 6cm is spaced apart between hole, 3-4 batten is placed in bottom bubble chamber, encloses one deck sunshade net, successively spread wetting cultivation matrix;
B. the plantlet in vitro through strong seedling culture is washed away agar base portion to cut off, after pretreatment fluid dipping 20s ~ 30s of taking root, directly transplant in the cultivation matrix in bubble chamber, plant interval 3 ~ 5cm;
C. after plantlet in vitro is transplanted, every morning 9 and afternoon, 5 atomizings were watered 2 times, noon, Stoma of Leaves closed, affect plantlet in vitro blade and absorb moisture, plantlet in vitro blade executes 1 foliage fertilizer in every 3 days after growing root system, execute weekly 1 special efficacy fertilizer, after 15 days, spraying in every two weeks 1 low concentration tpn, carbendazim, thiophanate methyl are with preventing disease and pest.
Further, the medium in described step (1) is the WPM medium containing plant hormone after improvement, and plant hormone is zeatin concentration is 1.0mg/L.
Further, the cultivation matrix in described step (2) to be ratio be 1: 1 peat soil and perlite mixture.
Further, the pretreatment fluid of taking root in described step (2) is the mixed solution of indolebutyric acid, VB1 and boric acid, and wherein the concentration of indolebutyric acid is the concentration of 1000mg/L, VB1 is 100mg/, and the concentration of boric acid is 20mg/L.
" Ao Ni'er " blueberry is the kind that North Carolina in 1987 is delivered, and has precocity, fruit grain is large, thick flavor, local flavor are good, base of fruit trace is little, the feature of rapid-curing cutback, storage tolerance, be one of Representative Cultivars in southern high clump blueberry, have development potentiality.Be applicable to cultivation in warm temperate zone to subtropical zone, its dormancy low temperature requirements meets southern weather conditions, both can not bloom because low temperature requirements cannot meet, and keep not being broken dormancy under the unusual high temperature that can may occur in the winter time again, off-season flowering does not occur.In addition stronger to the adaptability of soil.
The present invention with " Ao Ni'er " blueberry for material, by the research to its high-effective root-growing method, to explore the raise seedling method on scale of Ao Ni'er's blueberry, for Ao Ni'er's blueberry factorial seedling growth provides a kind of effective method.
Experiment one
The cultivation matrix No. 1 ~ No. 7 (as shown in table 1) of the peat soil of different proportion and perlite composition is utilized to cultivate the parameter situation (as shown in table 2) such as survival rate, plant height, leading thread, the number of blade of blueberry seedling by contrast, reach a conclusion, when cultivation matrix in the present invention is peat soil and the perlite mixture of ratio 1: 1, blueberry seedling percent is the highest, plant height, Aboveground Biomass of Young and Underground biomass are the highest, and leading thread and the number of blade are relatively high.Concrete effect is as follows:
Table 1 difference disinfects culture matrix
The impact of table 2 different culture media confrontation blueberry growth of seedling
Experiment two
Find that the rooting rate of the different pretreatment fluid dipping blueberry seedling that takes root is also different with root system quality by comparative trial.As shown in table 3, methyl α-naphthyl acetate, heteroauxin and the indolebutyric acid growth table to blueberry radical bud reveals low concentration and promotes, high concentration depression effect.When methyl α-naphthyl acetate, heteroauxin, indolebutyric acid concentration are 1000mgL-1, rooting rate and the amount of taking root all reach maximum, the quality that can strengthen radical bud of VB1, boric acid can strengthen the resistance of crops, adopt 1000mg/L indolebutyric acid+100mg/VB1+20mg/L boric acid to carry out culture of rootage, the sturdy prosperity of root system, fibrous root is many, quality is high, is of value to the growth of blueberry.
The different root-growing agent of table 3 is on the impact of rooting rate
For " Ao Ni'er " blueberry culture of rootage, the present invention adopts bubble chamber as incubator first, and coils cultivation with cave and compare.Found that, the gas permeability of cave dish is poor, medium easily hardens, the blueberry seedling growth of cultivation is small and weak, yellow leaf, root system development are bad, fibrous root is few, and adopts cave dish microbiological contamination rate high; And bubble chamber better, the subenvironment of gas permeability is stable after treatment, temperature, humidity are easy to control, cultivate blueberry seedling growth better, plant is sturdy, well developed root system, fibrous root are many, survival rate is cultivated higher than cave dish far away.
Beneficial effect of the present invention:
1. the present invention is after carrying out strong seedling culture, employing take root pretreatment fluid dipping 20s ~ 30s, pretreatment fluid of taking root is the mixed solution of indolebutyric acid, VB1 and boric acid, and wherein the concentration of indolebutyric acid is 1000mg/L, the concentration of VB1 is 100mg/, and the concentration of boric acid is 20mg/L.Add VB1 and boric acid, the quality of root and the resistance of plant can be strengthened to a certain extent, reduce and cultivate link, and blueberry tissue culture seedling cuttage survival rate is improved significantly after treatment, shorten cultivation period, reduce production cost, be beneficial to and carry out large-scale production.
2. when the cultivation matrix in the present invention is peat soil and the perlite mixture of ratio 1: 1, blueberry seedling percent is the highest, and plant height, Aboveground Biomass of Young and Underground biomass are the highest, and leading thread and the number of blade are relatively high.
3. the present invention adopts new-type incubator---bubble chamber carries out field planting to blueberry seedling, cultivate blueberry seedling growth better, plant is sturdy, well developed root system, fibrous root are many, survival rate is high.
Embodiment
A high-effective root-growing method for blueberry tissue culture seedling, carry out according to following steps:
(1) strong seedling culture:
A., after choosing the shoot tip meristem segment of blueberry plant, sterilization, be seeded in and sprout in having in the bottle of medium to cultivate, cultivation temperature is 25 ± 2 DEG C, relative moisture > 95%, utilizes double-deck shade net to shelter from heat or light, and places and cultivates about 30d;
B. choose the bottle seedling grown fine, height, at more than 5cm, connects bottle and moves to green house, carry out strong seedling culture, cultivation temperature is 25 ± 2 DEG C, relative moisture > 95%, cultivate under illumination 2500-3000LX, light application time is 10-12h/d, places and cultivates about 30d;
(2) culture of rootage:
A. make the bubble chamber that length, width and height are respectively 54cm, 40cm, 25cm, bubble chamber bear, the diameter in hole is 2cm, 6cm is spaced apart between hole, 3-4 batten is placed in bottom bubble chamber, encloses one deck sunshade net, successively spread wetting cultivation matrix;
B. the plantlet in vitro through strong seedling culture is washed away agar base portion to cut off, after pretreatment fluid dipping 20s ~ 30s of taking root, directly transplant in the cultivation matrix in bubble chamber, plant interval 3 ~ 5cm;
C. after plantlet in vitro is transplanted, every morning 9 and afternoon, 5 atomizings were watered 2 times, within every 3 days after growing root system, execute 1 foliage fertilizer, execute weekly 1 special efficacy fertilizer, after 15 days, spraying in every two weeks 1 low concentration tpn, carbendazim, thiophanate methyl are with preventing disease and pest.
Medium in described step (1) is the WPM medium containing plant hormone after improvement, and plant hormone is zeatin concentration is 1.0mg/L.
Cultivation matrix in described step (2) to be ratio be 1: 1 peat soil and perlite mixture.
Pretreatment fluid of taking root in described step (2) is the mixed solution of indolebutyric acid, VB1 and boric acid, and wherein the concentration of indolebutyric acid is the concentration of 1000mg/L, VB1 is 100mg/, and the concentration of boric acid is 20mg/L.
Claims (4)
1. a high-effective root-growing method for blueberry tissue culture seedling, is characterized in that, carries out according to following steps:
(1) strong seedling culture:
A., after choosing the shoot tip meristem segment of blueberry plant, sterilization, be seeded in and sprout in having in the bottle of medium to cultivate, cultivation temperature is 25 ± 2 DEG C, relative moisture > 95%, utilizes double-deck shade net to shelter from heat or light, and places and cultivates about 30d;
B. choose the bottle seedling grown fine, height, at more than 5cm, connects bottle and moves to green house, carry out strong seedling culture, cultivation temperature is 25 ± 2 DEG C, relative moisture > 95%, cultivate under illumination 2500-3000LX, light application time is 10-12h/d, places and cultivates about 30d;
(2) culture of rootage:
A. make a bubble chamber, bubble chamber bear, the diameter in hole is 1-3cm, is spaced apart 5-7cm between hole, 3-4 root batten is placed in bottom bubble chamber, encloses one deck sunshade net, successively spread wetting cultivation matrix;
B. the plantlet in vitro through strong seedling culture is washed away agar, base portion is cut off, and after pretreatment fluid dipping 20s ~ 30s of taking root, directly transplants in the cultivation matrix in bubble chamber, plant interval 3 ~ 5cm;
C. after plantlet in vitro is transplanted, every morning 9 and afternoon, 5 atomizings were watered 2 times, within every 3 days after growing root system, execute 1 foliage fertilizer, execute weekly 1 special efficacy fertilizer, after 15 days, spraying in every two weeks 1 low concentration tpn, carbendazim, thiophanate methyl are with preventing disease and pest.
2. the high-effective root-growing method of a kind of blueberry tissue culture seedling according to claim 1, is characterized in that, the medium in described step (1) is the WPM medium containing plant hormone, and plant hormone is zeatin, and zeatin concentration is 1.0mg/L.
3. the high-effective root-growing method of a kind of blueberry tissue culture seedling according to claim 1, is characterized in that, the cultivation matrix in described step (2) to be ratio be 1: 1 peat soil and perlite mixture.
4. the high-effective root-growing method of a kind of blueberry tissue culture seedling according to claim 1, it is characterized in that, pretreatment fluid of taking root in described step (2) is the mixed solution of indolebutyric acid, VB1 and boric acid, wherein the concentration of indolebutyric acid is 1000mg/L, the concentration of VB1 is 100mg/, and the concentration of boric acid is 20mg/L.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657144A (en) * | 2020-06-24 | 2020-09-15 | 广西壮族自治区中国科学院广西植物研究所 | Tissue culture rapid propagation method of Nangao blueberry variety' Aunier |
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| CN102668959A (en) * | 2012-06-14 | 2012-09-19 | 浙江师范大学 | Rapid ex vitro rooting method for blueberry tissue culture seedlings and rooting culture matrix |
| CN104025832A (en) * | 2013-09-17 | 2014-09-10 | 天津市林业果树研究所 | Green branch cuttage method for blueberries in Tianjin area |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668959A (en) * | 2012-06-14 | 2012-09-19 | 浙江师范大学 | Rapid ex vitro rooting method for blueberry tissue culture seedlings and rooting culture matrix |
| CN104025832A (en) * | 2013-09-17 | 2014-09-10 | 天津市林业果树研究所 | Green branch cuttage method for blueberries in Tianjin area |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111657144A (en) * | 2020-06-24 | 2020-09-15 | 广西壮族自治区中国科学院广西植物研究所 | Tissue culture rapid propagation method of Nangao blueberry variety' Aunier |
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