CN112673945B - Method for enhancing aluminum resistance of peas - Google Patents
Method for enhancing aluminum resistance of peas Download PDFInfo
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- CN112673945B CN112673945B CN202011390281.8A CN202011390281A CN112673945B CN 112673945 B CN112673945 B CN 112673945B CN 202011390281 A CN202011390281 A CN 202011390281A CN 112673945 B CN112673945 B CN 112673945B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a method for enhancing pea aluminum resistance, which applies GR24 solution with the concentration of 30-150 nmol/L to pea plants. Compared with the prior art, the GR24 is added in the low-acid adaptation treatment step, so that the elongation of the root system can be obviously promoted, and the aluminum accumulation in the root zone, especially the aluminum content in the root tip transition zone, can be effectively reduced. Reduce the root tip transition zone callose accumulation that leads to by the aluminium toxicity, effectively reduce the content of root tip ROS, reduce the cell programmed death speed that ROS leads to and promote root system to grow to reach the effect of alleviating the aluminium toxicity, improve the resistant aluminium nature of plant.
Description
Technical Field
The invention relates to the technical field of planting, in particular to a method for enhancing aluminum resistance of peas.
Background
Aluminum is the most abundant metal element in the crust and accounts for 7.45% of the total weight of the crust, wherein 50% of the acid arable land is poisoned by aluminum. Aluminum toxicity is a main inhibiting factor for the growth of plants in acid soil, and aluminum can influence the growth of crop roots at the nanomolar concentration level, and is mainly characterized in that main roots become thick and short, root tips expand and brown, lateral roots and root hairs are reduced, root tips are bent and irregularly hooked, root crowns fall off, and the biological oxidation capacity of the roots is obviously reduced. The aluminum toxicity seriously limits the growth of crops, reduces the yield of the crops and has great influence on world grains.
Disclosure of Invention
The invention discloses a method for enhancing aluminum resistance of peas, which aims to solve one or more technical problems in the prior art and provide at least one beneficial choice or creation condition.
A method for enhancing the aluminum resistance of peas comprises the step of applying GR24 solution with the concentration of 30-150 nmol/L to pea plants.
The specific method for enhancing the aluminum resistance of peas comprises the following steps:
1) Seed soaking and germination accelerating: soaking peas in 5-10% NaClO solution for 30min to sterilize and accelerate germination, cleaning with sterile ultrapure water for several times at an interval of 5min, removing impurities, and adding into 0.5-0.6 mmol/L CaCl 2 Soaking the seeds in the solution for 10 to 12 hours, keeping the temperature at 24 +/-2 ℃ and keeping the temperature in a dark roomConditions;
2) Aeroponics: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5-1.0 mmol/L CaCl into the aeroponic box 2 Immersing the solution in an atomizing culture device, atomizing and culturing for 48-72h 2 The solution is replaced every 24 h; caCl appears in the process 2 When the solution is not enough (the exposure of the pea seeds exceeds 1/3), the pea seeds need to be replenished and topped up;
3) Water culture: after the pea seeds germinate, selecting seedlings with consistent root length and 3-5 cm high, preparing Hoagland nutrient solution with the concentration of 1/4-1/2 as culture solution A, and soaking the roots of the seedlings with the culture solution; and the culture solution A is soaked for more than 24 hours;
4) Low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1-0.2 mmol/L CaCl 2 0.3-0.6 mmol/L KCl and 30-150 nmol/L GR24, and the pH is 4.5.
Compared with the prior art, the GR24 is added in the low-acid adaptation treatment step, so that the elongation of the root system can be obviously promoted, and the aluminum accumulation in the root zone, especially the aluminum content in the root tip transition zone, can be effectively reduced. Reduce the root tip transition zone callose accumulation that leads to by the aluminium toxicity, effectively reduce the content of root tip ROS, reduce the cell programmed death speed that ROS leads to and promote root system to grow to reach the effect of alleviating the aluminium toxicity, improve the resistant aluminium nature of plant.
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FIG. 1 is a graph comparing the relative elongation of lateral roots after GR24 treatment with different concentrations as described in example 5;
FIG. 2 is a comparison of the staining of example 3 with the staining of comparative example 1 with hematoxylin at the root tip;
FIG. 3 is a graph comparing the measurement of aluminum content in example 3 with that in comparative example 1;
FIG. 4 is a comparison of root tip Morin staining of example 3 and comparative example 1;
FIG. 5 is a graph comparing the amount of callose accumulated at the root tip in example 3 with that in comparative example 1;
FIG. 6 is a graph comparing the root tip ROS (reactive oxygen species) levels of example 3 and comparative example 1.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below.
Example 1
1) Seed soaking and germination accelerating: soaking peas in 7.5% NaClO solution for 30min for disinfection and germination, washing with sterile ultrapure water for 5 times, washing for 5min, removing impurities, and adding 0.5mmol/L CaCl 2 Soaking the seeds in the solution for 10 hours, and keeping the temperature at 24 ℃ under the dark room condition;
2) Aeroponics: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5mmol/L CaCl into the aeroponic box 2 Immersing the solution in an atomizer, atomizing and culturing for 48h when CaCl is added 2 When the solution is not enough, it needs to be filled with CaCl 2 The solution is replaced every 24 h;
3) Water culture: after the pea seeds germinate, selecting seedlings with consistent root length growth and 4 +/-0.1 cm high, preparing Hoagland nutrient solution with the concentration of 1/4 as culture solution A, and soaking the roots of the seedlings with the culture solution; and the culture solution A is soaked for 24 hours;
4) Low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1mmol/L CaCl 2 0.5mmol/L KCl and 30nmol/L GR24, pH 4.5; the low acid is adapted for 10h.
Example 2
1) Seed soaking and germination accelerating: soaking peas in 7.5% NaClO for 30min for disinfection and germination, washing with sterile ultrapure water 5 times, washing once in 5min, removing impurities, and adding 0.5mmol/L CaCl 2 Soaking the seeds in the solution for 10 hours, and keeping the temperature at 24 ℃ under the dark room condition;
2) Aeroponics: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5mmol/L CaCl into the aeroponic box 2 Immersing the solution in an atomizer, atomizing and culturing for 48h when CaCl is added 2 When the solution is not enough, it needs to be filled with CaCl 2 The solution is replaced every 24 h;
3) Water culture: after the pea seeds germinate, selecting seedlings with consistent root length and height of 4 +/-0.1 cm, preparing a Hoagland nutrient solution with the concentration of 1/4 as a culture solution A, and soaking the roots of the seedlings in the culture solution; and the culture solution A is soaked for 24 hours;
4) Is low withAcid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1mmol/L CaCl 2 0.5mmol/L KCl and 60nmol/L GR24, pH 4.5; the low acid is adapted for 10h.
Example 3
1) Seed soaking and germination accelerating: soaking peas in 7.5% NaClO solution for 30min for disinfection and germination, washing with sterile ultrapure water for 5 times, washing for 5min, removing impurities, and adding 0.5mmol/L CaCl 2 Soaking the seeds in the solution for 10 hours, and keeping the temperature at 24 ℃ under the dark room condition;
2) Aeroponics: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5mmol/L CaCl into the aeroponic box 2 Immersing the solution in an atomizer, atomizing and culturing for 48h when CaCl is added 2 When the solution is not enough, it needs to be filled with CaCl 2 The solution is replaced every 24 hours;
3) Water culture: after the pea seeds germinate, selecting seedlings with consistent root length and height of 4 +/-0.1 cm, preparing a Hoagland nutrient solution with the concentration of 1/4 as a culture solution A, and soaking the roots of the seedlings in the culture solution; and the culture solution A is soaked for 24 hours;
4) Low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1mmol/L CaCl 2 0.5mmol/L KCl and 125nmol/L GR24, pH 4.5; the low acid is adapted for 10h.
Comparative example 1
1) Seed soaking and germination accelerating: soaking peas in 7.5% NaClO solution for 30min for disinfection and germination, washing with sterile ultrapure water for 5 times, washing for 5min, removing impurities, and adding 0.5mmol/L CaCl 2 Soaking the seeds in the solution for 10 hours, and keeping the temperature at 24 ℃ under the dark room condition;
2) Aeroponics: putting the pea seeds fully swelled into an aeroponics box, and adding 0.5mmol/L CaCl into the aeroponics box 2 Immersing the solution in an atomizer, atomizing and culturing for 48h when CaCl is added 2 When the solution is not enough, it needs to be filled with CaCl 2 The solution is replaced every 24 hours;
3) Water culture: after the pea seeds germinate, selecting seedlings with consistent root length and height of 4 +/-0.1 cm, preparing a Hoagland nutrient solution with the concentration of 1/4 as a culture solution A, and soaking the roots of the seedlings in the culture solution; and the culture solution A is soaked for 24 hours;
4) Low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1mmol/L CaCl 2 0.5mmol/L KCl, pH 4.5; the low acid is adapted for 10h.
Example 5
The aluminum-resistant treated pea seedlings obtained in examples 1 to 3 and comparative example 1 were each subjected to aluminum treatment. So as to contain 0.1mmol/L CaCl 2 ，0.5mmol/L KCl，15μmol/L AlCl 3 Soaking the culture solution for 24h, and then respectively carrying out lateral root length measurement, root tip hematoxylin staining, aluminum content measurement, root tip Morin staining, root tip callose accumulation amount observation and root tip ROS (reactive oxygen species) level measurement. The results are shown in FIGS. 1 to 6. GR24 at various concentrations all served to enhance the aluminum resistance of peas, but the preferred value was 125nmol/L. Thus, subsequent comparisons were made with example 3 as the "+ GR24" sample and comparative example 1 as the "-GR24" sample.
In the embodiments 1 to 3, the GR24 with different concentrations is respectively adopted for low-acid adaptive treatment, and comparison shows that the pretreatment by the GR24 can effectively reduce the content of aluminum in the root tip, simultaneously reduce the generation of callose and active oxygen in the root tip under the aluminum stress, and relieve the inhibition of the aluminum on the root elongation, thereby achieving the effect of relieving the aluminum toxicity and improving the aluminum resistance of plants.
Claims (6)
1. A method of enhancing the aluminum resistance of peas comprising the steps of:
1) Seed soaking and germination accelerating;
2) Carrying out aeroponic culture;
3) Water culture: preparing a Hoagland nutrient solution with the concentration of 1/4-1/2 as a culture solution A, and immersing the roots of the seedlings after the pea seeds germinate in the culture solution A;
4) Low-acid adaptation treatment: when the length of the maximum lateral root of the seedling reaches 1-2 cm, changing the culture solution A into a culture solution B; the culture solution B contains 0.1-0.2 mmol/L CaCl 2 0.3-0.6 mmol/L KCl and 30-150 nmol/L GR24, pH 4.5, at least 24h.
2. The method of claim 1, wherein step 1) comprises: soaking peas in 5-10% of NaClO for 10-60 min for disinfection and germination acceleration, then washing with sterile ultrapure water for several times, removing impurities, and adding 0.5-0.6 mmol/L CaCl 2 Soaking the seeds in the solution for 10 to 12 hours, and keeping the temperature at 24 +/-2 ℃ under the dark room condition.
3. The method of claim 1, wherein step 2) comprises: putting the pea seeds fully imbibed into an aeroponic box, and adding 0.5-1.0 mmol/L CaCl into the aeroponic box 2 Immersing the solution in an aeroponics device, and carrying out aeroponics culture for 48-72 h.
4. The method of claim 3, wherein the CaCl of step 2) is 2 The solution was changed every 24h.
5. The method as claimed in claim 1, wherein the seedlings of step 3) are required to have consistent root length and height of 3-5 cm.
6. The method according to claim 1, wherein the conditions for replacing culture solution A with culture solution B in step 4) are as follows: the maximum side root length of the seedling reaches 1-2 cm.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010128112A2 (en) * | 2009-05-07 | 2010-11-11 | Gmi - Gregor-Mendel-Institut Für Molekulare Pflanzenbiologie Gmbh | Use of strigolactones |
| CN108040844A (en) * | 2017-11-15 | 2018-05-18 | 佛山科学技术学院 | A kind of aluminum-resistant poison pea seedling inoculation method |
| CN111802393A (en) * | 2020-07-30 | 2020-10-23 | 青岛农业大学 | Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof |
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| CN104542587B (en) * | 2014-12-18 | 2016-06-08 | 昆明理工大学 | Chlorogenic acid application in alleviating soybean seedling aluminium toxicity |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010128112A2 (en) * | 2009-05-07 | 2010-11-11 | Gmi - Gregor-Mendel-Institut Für Molekulare Pflanzenbiologie Gmbh | Use of strigolactones |
| CN108040844A (en) * | 2017-11-15 | 2018-05-18 | 佛山科学技术学院 | A kind of aluminum-resistant poison pea seedling inoculation method |
| CN111802393A (en) * | 2020-07-30 | 2020-10-23 | 青岛农业大学 | Novel compound for improving soil aluminum toxicity resistance of plants and preparation method thereof |
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