KR101957327B1 - Methods of growing ginseng seeds for shortening dormancy period - Google Patents

Abstract

The present invention relates to a method of growing ginseng seeds that shortens the sleeping period, and more particularly, to a method of growing ginseng seeds by dipping a ginseng seed in a solution of gibberellic acid (GA); Cultivating with a mixed solution of CRMG (Cultured Roots of Mountain Ginseng) nutrient and GA solution; Lt; RTI ID = 0.0 > 15 C < / RTI > Immersing in a kinetin solution; And a step of sowing in the soil, a method of growing ginseng seeds comprising the step of growing the ginseng seeds, and a method of growing the ginseng seeds.
The growth method of ginseng according to the present invention can remarkably shorten the duration of dormancy of ginseng seeds and increase the growth of ginseng germinated, so that it can be widely used for ginseng cultivation.

Description

[0001] The present invention relates to a method of growing ginseng seeds for shortening dormancy period,

The present invention relates to a method of growing ginseng seeds that shortens the sleeping period, and more particularly, to a method of growing ginseng seeds by dipping a ginseng seed in a gibberellic acid (GA) solution; Cultivating with a mixed solution of CRMG (Cultured Roots of Mountain Ginseng) nutrient and GA solution; Lt; RTI ID = 0.0 > 15 C < / RTI > Immersing in a kinetin solution; And a step of sowing in the soil, a method of growing ginseng seeds comprising the step of growing the ginseng seeds, and a method of growing the ginseng seeds.

Ginseng is a remnant of the geological era and has strong cold resistance and strong independence. It is a unique plant that breaks down without lowering the stalks until it is wounded or the growth environment is poor, and the growth rate is slow and the fertility is weak. It is also a semi-shrub plant that grows in the shade of a tree, can not grow in places where sunlight is too high, and is not found in places where humidity is too high or too low. Growing conditions are very difficult, and coniferous forests such as pine and fir can not grow in the shade year round, and soils prefer the place where the leaves are corroded and the drainage is good.

In general, ginseng seed immediately after seeding is not germable because the immature stomach is surrounded by hard transdermal patches. Therefore, the seedlings were cultivated in July, and dehiscence was applied by layering method for about 3 months (90 days) from the end of July to the beginning of August. The seeds of ginseng will germinate only after March after the seeds are sown in early November and after receiving the low temperature response for about 3 months of wintering. Thus, germination of ginseng seeds can be accomplished by breaking the triple dormancy composed of transdermal seed, immature embryo, and low temperature response. In addition, ginseng seeds are characterized by low storage stability and low germination rate. In the case of storage as unopened shell after harvesting of ginseng seed, the degree of decapitation was reduced to 38.7% after 1 year of storage, and even when stored in a storage tank (5 ° C, 30% humidity) ) Was reported to decrease (Korean Ginseng Journal, Vol. 28, No. 4, p.215-218).

As a result, techniques for promoting the opening of the gums such as the ginseng seedling deafening apparatus (Korean Patent Registration No. 10-1589215) and the ginseng seed automation opening apparatus (Korean Patent Laid-Open No. 10-2015-0055483) Growth of seeds such as ginseng sprout cultivation method using LED light (Korean Patent Registration No. 10-1319082) and organic ginseng cultivation method using indigenous microbial culture solution (Korean Patent Registration No. 10-2013-0078366) Has been developed. However, there is still a need for studies to establish optimal grooming conditions and growth conditions to shorten the dormancy period of ginseng seeds and increase germination efficiency.

Under these circumstances, the inventors of the present invention have made extensive efforts to develop a method for growing ginseng seeds promoting the decomposition of ginseng seeds and the growth of ginseng seeds. As a result, it has been found that a gibberellic acid solution, a cultured ginseng root culture filtrate, a kinetin solution, The inventors of the present invention have completed the present invention by confirming that the growth of germinated ginseng seeds is enhanced by promoting the opening of the ginseng seeds when they are subjected to such steps as immersion, rooting, low temperature treatment and growth using soil or the like.

One object of the present invention is to provide a method for producing ginseng seeds, comprising the steps of: (a) immersing ginseng seeds in a solution of gibberellic acid (GA); (b) cultivating the seed having been immersed in a mixed solution of CRMG (Cultured Roots of Mountain Ginseng) nutrient and GA solution; (c) low-temperature treatment of the seeds having been subjected to deionization at 5 to 15 캜; (d) immersing the seed in which the low temperature treatment has been completed in a kinetin solution; And (e) a step in which the seed having been immersed in the soil and the soil containing the Vermiculite: Perlite: Peat Moss at a volume ratio of 2.5: 3.5: 0.5 to 1.5: 0.5: : 0.5 to 1.5 in a volume ratio of 1: 1 to 2: 1.

Another object of the present invention is to provide ginseng seeds grown through the above growth method.

It is another object of the present invention to provide a soil composition for growing the ginseng seed.

In order to accomplish the above object, one aspect of the present invention is a method for manufacturing a ginseng seed, comprising the steps of: (a) immersing ginseng seed in a solution of gibberellic acid (GA); (b) cultivating the seed having been immersed in a mixed solution of CRMG (Cultured Roots of Mountain Ginseng) nutrient and GA solution; (c) low-temperature treatment of the seeds having been subjected to deionization at 5 to 15 캜; (d) immersing the seed in which the low temperature treatment has been completed in a kinetin solution; And (e) a step in which the seed having been immersed in the soil and the soil containing the Vermiculite: Perlite: Peat Moss at a volume ratio of 2.5: 3.5: 0.5 to 1.5: 0.5: : 0.5 to 1.5 in a volume ratio. The method of growing ginseng seeds according to claim 1,

The method of growing ginseng seeds according to the present invention can achieve expected effects such as shortening the dormancy period of the ginseng seed, promoting the decolletage, and increasing the growth, when the steps (a) to (e) have.

Generally, ginseng seeds germinate only after about 3 months of seedling treatment is completed and after about 3 months of low temperature treatment. In the present invention, it was confirmed that when the ginseng seed was grown at 25 ° C using a mixture of GA (Gibberellic Acid) solution and CRMG (Cultured Roots of Mountain Ginseng) nutrient solution and GA solution, , It was confirmed that germination was achieved within 28 days when the seeds were treated at low temperature at 10 ° C. The seeds having been subjected to the open and low temperature treatments were mixed at a ratio of 1: 1 with a soil containing kinetin solution and Vermiculite: Perlite: Peat Moss at a volume ratio of 3: 1: 1, the germination rate was significantly increased in the mixed soil.

The term "ginseng seed" of the present invention means seeds of ginseng. It is common for the seeds harvested from ginseng to be harvested once in 4 years old ginseng. Since ginseng seed immediately after the seedling is immersed in hard transdermal patches, it is impossible to germinate. Therefore, the seeds are sown in the soil after opening the seedling to facilitate germination by appropriately controlling moisture and temperature.

The term "GA (Gibberellic Acid) solution" of the present invention means a solution containing gibberellic acid, which is a plant hormone.

The term " CRMG (Cultured Roots of Mountain Ginseng) nutrient "of the present invention refers to culture filtrate remaining after culture of Cultured Roots of Mountain Ginseng (CRMG) is cultured. The cultivation of the wild ginseng cultured muscle is characterized in that it can be safely used as a pollution-free raw material because it is cultured in a sterilized state using a bioreactor. In particular, CRMG culture filtrate is generally discarded after use. However, in the present invention, CRMG culture filtrate can be used for growth of ginseng seeds for the first time. CRMG culture filtrate is used to shorten the dormancy period of the ginseng seed, In the first place.

The term "kinetin solution" of the present invention means a solution containing kinetin, which is a plant hormone.

In the present invention, the above steps (a) and (b) are the step of treating the ginseng seeds. As used herein, the term "open armor " means that the seed husks are spread by growing the seeds of the ginseng seeds. In the present invention, the term " dormancy breaking "

Specifically, the step (a) is a step of immersing the ginseng seed in the GA solution, wherein the concentration of the GA solution used may be 90 to 110 ppm, specifically 95 to 105 ppm, more specifically 100 ppm. The immersion may also be carried out for 20 to 30 hours, specifically 22 to 28 hours, more particularly 24 hours.

The step (b) is a process for controlling the ginseng seed, wherein the cross-over solution used may be a mixed solution of the CRMG nutrient solution and the GA solution.

At this time, the concentration of the GA solution may be 5 to 15 ppm, specifically 7 to 13 ppm, more specifically 10 ppm. In addition, the CRMG nutrient may be diluted with 90 to 110 times the volume of the CRMG culture filtrate, and more specifically, 100 times the volume ratio. In addition, the step (b) may be carried out in a chamber, wherein the temperature of the incubator may be 20 to 30 ° C, specifically 25 ° C, and the process may be carried out for 40 to 45 days, more specifically for 42 days .

In one specific embodiment of the present invention, the ginseng seeds were immersed in a GA solution at a concentration of 100 ppm for 24 hours, and then incubated for 42 days in a mixed solution of CRMG nutrient diluted 100 times in a 25 ° C. incubator and 10 ppm GA solution It was confirmed that the degree of opening of the ginseng seeds was maximized in the case where the animals were inhabited (Table 2 and Figs. 5 to 7). This suggests that performing the method according to the present invention can significantly shorten the decantering period of the ginseng seed, compared with the case where the general decanter treatment is performed for about 90 days.

In the present invention, step (c) is a step of low temperature treatment of ginseng seed. Specifically, the step of placing the ginseng seeds of the step (a) and the step (b) in a low temperature is performed at 5 to 15 ° C, more specifically, at 10 ° C. The process can also be carried out for 25 to 30 days, specifically 28 days.

In a specific example of the present invention, it was confirmed that the germination rate was increased when the ginseng seeds that had been subjected to decanter treatment were subjected to a low temperature treatment at 10 ° C for 28 days, compared with the case where they were conducted at a general low temperature treatment temperature of 4 ° C (FIGS. . This suggests that when the general low temperature treatment is performed for about 90 days, the method according to the present invention can significantly shorten the low temperature treatment period of the ginseng seed.

In the present invention, steps (d) and (e) above are the step of promoting the growth of ginseng seeds.

Specifically, the step (d) is a step of immersing the ginseng seeds after completion of the decolorization and the low temperature treatment of (a) to (c) in a kinetin solution, wherein the concentration of the kinetin solution used is 40 to 60 ppm, Lt; / RTI > The immersion may also be carried out for 20 to 30 hours, specifically 22 to 28 hours, more particularly 24 hours.

In a specific example of the present invention, it was confirmed that when the ginseng seeds having been subjected to the open mouth treatment and the low temperature treatment were immersed in the kinetin solution, the germination rate was increased to about two times or more as compared with the case of immersion in water (Tables 3 to 5) . This suggests that ginseng seed germination rate can be remarkably increased by performing the kinetin immersion step according to the present invention.

The step (e) is a step of sowing the seeds of the ginseng seeds, which have been subjected to the decolorization, the low temperature and the dipping treatment of (a) to (d), into the soil, wherein the soil used is Vermiculite: Perlite: Soil and rutto soil containing peat moss at a volume ratio of 2.5 to 3.5: 0.5 to 1.5: 0.5 to 1.5, in particular 3: 1: 1, and 0.5 to 1.5: 0.5 to 1.5, By volume ratio. Soil containing vermiculite: pearlite: peat moss at a volume ratio of 3: 1: 1 has a low specific gravity. As a result, when the water is sprayed, the seeds float in water and sink, But it was not suitable for growth. In the present invention, the above-mentioned disadvantages are overcome by mixing the soil with the rust-soot.

In one specific embodiment of the present invention, soil type-1 mixed with vermiculite, perlite and peat moss; And soil type-2 were mixed to produce an artificial mixed soil (soil type-3). When the germinated seeds were used for the growth of ginseng seeds, germination rate was higher than that of soil type-1 or soil type- (Table 3 to 5). ≪ tb > < TABLE > This suggests that the germination rate of ginseng seeds can be remarkably increased if the ginseng seeds are sown on the soil type-3 according to the present invention.

Another embodiment provides the ginseng seeds grown through the above growth method.

At this time, the definition of the "ginseng seed" is as described above.

Another embodiment provides a soil composition for growing the ginseng seed.

At this time, the definition of the "ginseng seed" is as described above.

In the present invention, the soil composition comprises Vermiculite: Perlite: Peat Moss in a volume ratio of 2.5: 3.5 to 0.5: 1.5: 0.5 to 1.5, specifically 3: 1: 1 The soil and the rust soot may be mixed in a volume ratio of 0.5 to 1.5: 0.5 to 1.5, specifically 1: 1. Soil containing vermiculite: pearlite: peat moss at a volume ratio of 3: 1: 1 has a low specific gravity. As a result, when the water is sprayed, the seeds float in water and sink, But it was not suitable for growth. The present invention overcomes the disadvantages of mixing the soil with the rust soot, and the soil mixed with the soil mixed with the vermiculite, the pearlite and the peat moss has the effect of increasing the germination rate of the ginseng seed Respectively.

In one specific embodiment of the present invention, soil type-1 mixed with vermiculite, perlite and peat moss; And soil type-2 were mixed to produce an artificial mixed soil (soil type-3). When the germinated seeds were used for the growth of ginseng seeds, germination rate was higher than that of soil type-1 or soil type- (Table 3 to 5). ≪ tb > < TABLE > This suggests that the soil composition according to the present invention can remarkably increase the germination rate of ginseng seeds and thus can be usefully used for the growth of ginseng seeds.

The growth method of ginseng according to the present invention can remarkably shorten the duration of dormancy of ginseng seeds and increase the growth of ginseng germinated, so that it can be widely used for ginseng cultivation.

FIG. 1 is a schematic diagram showing an experimental procedure for shortening the period of dormancy break.
Fig. 2 is an image showing the degree of fold increase of ginseng seeds treated with decanter for 14 days at 10 占 폚. At this time, GA, CRMG and GA + CRMG are a mixture of water, Gibberellic Acid, wild ginseng root nutrient solution and GA and CRMG nutrients as control solution conditions.
FIG. 3 is an image showing the degree of fold increase of ginseng seeds treated at 10 ° C for 28 days.
Fig. 4 is an image showing the degree of fold increase of ginseng seeds treated at 10 캜 for 42 days.
FIG. 5 is an image showing the degree of fold increase of ginseng seeds treated at 15 ℃ for 14 days.
FIG. 6 is an image showing the degree of fold increase of ginseng seeds treated at 25 ° C for 28 days.
Fig. 7 is an image showing the degree of fold increase of ginseng seeds treated at 25 캜 for 42 days.
FIG. 8 is an image showing the degree of double extension of ginseng seeds which was carried out for 3 months (90 days) in the conventional method.
FIG. 9 is a graph showing germination percentage of ginseng seeds grown after 14 days of decolletage treatment according to each condition. FIG. At this time, w1 and w2 are germination rates after 1 week and 2 weeks after growth, respectively; 10C and 25C were growth temperature conditions of 10 DEG C and 25 DEG C, respectively; C and GA were used as immersion solution conditions, respectively. Water, Gibberellic Acid, C, GA, CRMG and GA + CRMG were mixed solution of water, Gibberellic Acid, do.
FIG. 10 is a graph showing the germination percentage of ginseng seeds grown after decarburization treatment for 28 days according to each condition. FIG.
Fig. 11 is a graph showing the germination percentage of ginseng seeds grown after decantering for 42 days according to each condition. Fig.
It is silica (SiO ₂₎ 12; And a mixture of a binder and PGPR (plant growth promoting rhizobacteria).
FIG. 13 is a schematic view showing the immersion, coating and sowing process of the ginseng seed which has been subjected to decanter treatment.

Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example  1. Optimal Open hand  Establish condition

Example  1-1. Open hand  And low-temperature treatment condition setting

Seed ginseng seeds were used from Kwon Hyun Kwon, a researcher of Oriental Biotechnology Research Center, Oriental Materials Engineering Department at Kyung Hee University. Daphne and K-1 varieties were used in the present invention. Table 1 below shows the treatments for the acceleration of acorns (shortening of dormancy period).

Maceration
(Performed for 24 hours) Temperature Rinsing
(Performed for 14 days, 28 days, and 42 days, respectively) Cold treatment conditions water 10 ℃ water 4 ° C or 10 ° C,
Observation on days 0, 14, and 28, respectively water 10 ℃ GA 10 ppm water 10 ℃ CRMG 100x dilution water 10 ℃ CRMG 100x dilution + GA 10 ppm GA 100 ppm 10 ℃ water 4 ° C or 10 ° C,
Observation on days 0, 14, and 28, respectively GA 100 ppm 10 ℃ GA 10 ppm GA 100 ppm 10 ℃ CRMG 100x dilution GA 100 ppm 10 ℃ CRMG 100x dilution + GA 10 ppm water 25 ℃ water 4 ° C or 10 ° C,
Observation on days 0, 14, and 28, respectively water 25 ℃ GA 10 ppm water 25 ℃ CRMG 100x dilution water 25 ℃ CRMG 100x dilution + GA 10 ppm GA 100 ppm 25 ℃ water 4 ° C or 10 ° C,
Observation on days 0, 14, and 28, respectively GA 100 ppm 25 ℃ GA 10 ppm GA 100 ppm 25 ℃ CRMG 100x dilution GA 100 ppm 25 ℃ CRMG 100x dilution + GA 10 ppm

Specifically, ginseng seeds were soaked in water and 100 ppm GA (Gibberellic Acid) solution for 24 hours, followed by shade for one day. Thereafter, the growth chamber was fixed at 10 ° C and 25 ° C for 2 days at 14 days and 28 days for 42 days, respectively. Water, 10 ppm GA, and Cultured Roots of Mountain Ginseng), CRMG nutrient diluted 100 times by sterilization of the remaining culture filtrate, and mixed nutrient solution containing CRMG nutrient and 10 ppm of GA were treated by treatment. The trail was run until the trailer had flowed fully under the tray. The control solution was recovered from the solution flowing under the tray, refrigerated and reused, and changed every week.

Then, the ginseng seeds with 14 days, 28 days, or 42 days after the cutting were taken out were weighed to determine the weight of each 100 lips. Then, 5 kinds of ginseng seeds were selected for each treatment, and the ginseng seeds were cut in half, Were observed with an optical microscope.

Finally, each treatment was treated for 0 days (without cold treatment), 14 days, or 28 days in incubator chambers fixed at 4 ° C and 10 ° C for cold treatment.

Thereafter, the germination rate was examined by dipping in one petri dish (ø 9 cm) with 3 repetitions of 25 leaves each. The teeth were prepared by placing two sheets of filter paper on a petri dish and then raising each of the low-temperature treated seeds that had been sterilized in 0.5% sodium hypochlorite for 10 minutes and 0.25% sodium hypochlorite for 10 minutes, The germination rate and the germination rate of the 2nd germination were investigated at 20 ℃, respectively. The germination was maintained in the dark state and germination was regarded as germination of 1 mm or more.

This experimental procedure is shown in Fig.

Example  1-2. Open hand  Treatment of ginseng seeds 100-lip  Check weight change

The ginseng seeds with 14 days, 28 days, or 42 days after cutting were taken out and weighed to determine the weight change. The results are shown in Table 2 below.

Maceration
(Performed for 24 hours) Temperature Rinsing 100 Lip Weight (g) 0 days 14 days 28th 42 days water 10 ℃ water 5.4 5.4 5.6 5.8 water 10 ℃ GA 10 ppm 5.6 5.6 5.7 5.7 water 10 ℃ CRMG 100x dilution 5.4 5.5 5.8 5.9 water 10 ℃ CRMG 100x dilution + GA 10 ppm 5.5 5.5 5.9 6.0 GA 100 ppm 10 ℃ water 5.5 5.5 5.8 5.9 GA 100 ppm 10 ℃ GA 10 ppm 5.5 5.5 6.0 6.0 GA 100 ppm 10 ℃ CRMG 100x dilution 5.6 5.6 5.9 5.9 GA 100 ppm 10 ℃ CRMG 100x dilution + GA 10 ppm 5.6 5.6 6.2 6.2 water 25 ℃ water 5.3 5.3 5.6 5.7 water 25 ℃ GA 10 ppm 5.3 5.3 5.6 5.8 water 25 ℃ CRMG 100x dilution 5.5 5.5 5.7 5.8 water 25 ℃ CRMG 100x dilution + GA 10 ppm 5.5 5.6 5.9 5.9 GA 100 ppm 25 ℃ water 5.8 5.8 6.1 6.2 GA 100 ppm 25 ℃ GA 10 ppm 5.8 5.8 6.3 6.2 GA 100 ppm 25 ℃ CRMG 100x dilution 5.9 6.1 6.4 6.4 GA 100 ppm 25 ℃ CRMG 100x dilution + GA 10 ppm 6.0 6.2 6.6 6.7

Specifically, it was confirmed that the weight of 100 gins of the ginseng seeds in each treatment was 5.4 g - 6.0 g before the treatment (day 0). The difference in the weight change of the 100 lips of the tea after 14 days treatment was not significantly different from that before the treatment. The difference in the weight changes after 28 days from the treatment of the open arm, Was found to be 5.7g - 6.7g. In particular, the treatments showing the largest difference in weight change relative to the weight of the 100 lips prior to the opening of the armor was that the ginseng seeds were immersed in a GA solution at a concentration of 100 ppm for 24 hours, shade for one day, CRMG nutrient diluted 100 times at 25 ° C, Of GA solution for 42 days, and the difference was 0.7g.

As a result, when the ginseng seeds were immersed in a GA solution at a concentration of 100 ppm for 24 hours and then cultivated for 42 days in a mixed solution containing a CRMG nutrient diluted 100 times at 25 ° C and a GA solution at a concentration of 10 ppm, Of the open mouth.

Example  1-3. Open hand By process  Identification of embryo development of ginseng seeds

The ginseng seeds were extracted from 14 days, 28 days, and 42 days after opening the leaves, and the ginseng seeds were selected for each treatments. The ginseng seeds were cut in half and the degree of extension of the ginseng was observed with an optical microscope.

Specifically, as can be seen from Figs. 2 to 4, the degree of growth of each treatment group grown at 10 占 폚 for 14 days, 28 days, and 42 days was confirmed.

On the other hand, as can be seen from Figs. 5 to 7, when the ginseng seeds were immersed in water, the growth of the ginseng seeds at 25 < 0 > C for 14 days, 28 days, However, in case of immersing in GA solution at 100 ppm concentration for 24 hours, it began to elongate after 28 days from the decolletage treatment, and it was confirmed that more than 1/3 of the seed length was elongated at 42 days. In particular, it was confirmed that the CRMG nutrient diluted 100 times and the GA solution 10 ppm were mixed, and that the growth of the embryo was extended by more than 1/2 of the seed length, (90 days) of the treatment.

As a result, when the ginseng seeds were immersed in a GA solution at a concentration of 100 ppm for 24 hours and then cultivated in a mixed solution containing a CRMG nutrient diluted 100 times at 25 DEG C for 42 days and a GA solution at a concentration of 10 ppm, And it could be shortened to about 48 days compared to the traditional open - mouth treatment period (about 90 days).

Example  1-4. Identification of germination rate of ginseng seeds at low temperature

Each of the treatments which had been treated for 42 days were subjected to low temperature treatment at 4 ° C and 10 ° C for 14 days and 28 days, respectively, and the germination rate thereof was confirmed.

Specifically, as can be seen from Figs. 9 to 11, the treatments (10C-C and 25C-C) in which the ginseng seeds were immersed in water as control were the treatments (10C-GA and 25C-GA) The germination rate was generally poor regardless of the low temperature treatment. (4C-14days and 4C-28days) compared with 10C treatment (10C-14days and 10C-28days) in comparison with the conventional case of low temperature treatment at 4 DEG C for 90 days. But the germination rate was rather low.

In particular, as shown in FIG. 11, the treatment showing the highest germination percentage was prepared by shaking ginseng seeds in a GA solution at a concentration of 100 ppm for 24 hours, shaking for a day, CRMG nutrient diluted 100 times at 25 ° C for 42 days, (25C-GA-CRMG + GA) treated with low temperature treatment at 10 ℃ for 28 days, and it was confirmed that the germination rate was 99%.

From the above results, it was found that the germination rate was increased when the ginseng seed was treated at low temperature for 28 days at 10 ° C, and it was found that the germination time could be shortened to about 28 days compared with the conventional low temperature treatment period (90 days).

Example  2. Establish optimal growth conditions

Example  2-1. Setting of growth condition

According to the results obtained in Example 1, in order to determine optimal growth conditions of the ginseng seeds having undergone dormancy breaking and low temperature treatment, the following conditions were set.

Specifically, the growth was examined by seed coating, treatment of growth regulator, soil condition, ginseng cultivar, and CRMG nutrient treatment. The humidity was 45% and the light intensity was fixed at 300 lux.

First, the dormant ginseng seeds were immersed in water or a kinetin solution as a growth regulator at a concentration of 50 ppm for 24 hours, and washed with running water for 3 hours.

In addition, coatings of ginseng seeds were 0.25 kg of silica (SiO ₂ ) in seeds of 0.5 kg of the Daesung cultivar and 0.5 kg of K-1 cultivars; And 90 ml of a binder and PGPR (plant growth promoting rhizobacteria). Silica coatings are known to be effective in preventing pests and resistance to stress, and PGPR is known to promote root growth and promote root growth. The appearance of coated ginseng seeds is shown in Fig.

The soil conditions were soil type-1 mixed with Vermiculite: Perlite: Peat Moss at a volume ratio of 3: 1: 1, (Soil type-2), which is made by crushing the soil type-1 and soil type-2, obtained by crushing the soil type-2 into fine particles, and a soil type- Respectively. After sterilization of the soil before sowing seeds, water was slowly and thoroughly given through bottom irrigation water at a height of 1/3 of the height of the tray to minimize and equalize the pores between the soil particles, and the soil depth was unified to 15 cm . The seeding interval was 2 cm for one compartment and 4 lines for a line spacing of 4 cm. The upper two lines were sown with the Daeung breed and the lower two lines were seeded with K-1.

The aforementioned immersion, coating and sowing processes are shown in Fig.

Finally, the irrigation method was sprayed with sterilized water or CRMG nutrient diluted 100 times every 3 days, and kinetin treated seeds were sprayed with keratin at a concentration of 50 ppm once every 3 days Lt; / RTI >

Example  2-2. Identification of growth condition according to growth condition

Tables 3 to 5 show the results of confirming the presence of seed coating, the presence or absence of treatment of growth regulators, the soil condition, the ginseng cultivar, and the germination rate according to the CRMG nutrient treatment.

Soil type kind Seed coating Immersion
(Maceration) Watering
(Flushing) Germination rate (%) 4 weeks 5 weeks 6 weeks 7 weeks Artificial soil
(soil type-1) Big wind X water water 0 0 0 0 Artificial soil Big wind O water water 0 0 0 0 Artificial soil Big wind X water CRMG nutrient 0 0 0 0 Artificial soil Big wind O water CRMG nutrient 0 0 0 0 Artificial soil K-1 X water water 0 0 0 0 Artificial soil K-1 O water water 0 0 0 5 Artificial soil K-1 X water CRMG nutrient 0 0 0 0 Artificial soil K-1 O water CRMG nutrient 0 0 0 0 Artificial soil Big wind X 50 ppm
Kinetin water 0 10 20 45 Artificial soil Big wind O 50 ppm kinetin water 0 0 0 15 Artificial soil Big wind X 50 ppm kinetin CRMG nutrient 0 0 20 30 Artificial soil Big wind O 50 ppm kinetin CRMG nutrient 0 0 0 5 Artificial soil K-1 X 50 ppm kinetin water 5 5 5 5 Artificial soil K-1 O 50 ppm kinetin water 0 0 0 0 Artificial soil K-1 X 50 ppm kinetin CRMG nutrient 0 15 20 20 Artificial soil K-1 O 50 ppm kinetin CRMG nutrient 0 0 0 5

Soil type kind Seed coating Immersion
(Maceration) Watering
(Flushing) Germination rate (%) 4 weeks 5 weeks 6 weeks 7 weeks Commercial soil
(soil type-2) Big wind X water water 0 0 0 0 Commercial soil Big wind O water water 0 0 0 0 Commercial soil Big wind X water CRMG nutrient 0 0 0 0 Commercial soil Big wind O water CRMG nutrient 0 0 0 0 Commercial soil K-1 X water water 0 0 0 0 Commercial soil K-1 O water water 0 0 0 0 Commercial soil K-1 X water CRMG nutrient 0 0 0 0 Commercial soil K-1 O water CRMG nutrient 0 0 0 0 Commercial soil Big wind X 50 ppm
Kinetin water 0 0 10 35 Commercial soil Big wind O 50 ppm kinetin water 0 0 10 20 Commercial soil Big wind X 50 ppm kinetin CRMG nutrient 0 5 5 20 Commercial soil Big wind O 50 ppm kinetin CRMG nutrient 0 0 0 5 Commercial soil K-1 X 50 ppm kinetin water 0 10 30 40 Commercial soil K-1 O 50 ppm kinetin water 0 0 0 0 Commercial soil K-1 X 50 ppm kinetin CRMG nutrient 0 10 15 25 Commercial soil K-1 O 50 ppm kinetin CRMG nutrient 0 0 0 5

Soil type kind Seed coating Immersion
(Maceration) Watering
(Flushing) Germination rate (%) 4 weeks 5 weeks 6 weeks 7 weeks Artificial mixed soil
(soil type-3) Big wind X water water 0 0 0 30 Artificial mixed soil Big wind O water water 0 0 0 5 Artificial mixed soil Big wind X water CRMG nutrient 0 0 0 15 Artificial mixed soil Big wind O water CRMG nutrient 0 0 0 0 Artificial mixed soil K-1 X water water 0 0 0 5 Artificial mixed soil K-1 O water water 0 0 0 0 Artificial mixed soil K-1 X water CRMG nutrient 0 0 5 5 Artificial mixed soil K-1 O water CRMG nutrient 0 0 0 0 Artificial mixed soil Big wind X 50 ppm
Kinetin water 5 15 70 85 Artificial mixed soil Big wind O 50 ppm kinetin water 0 0 10 15 Artificial mixed soil Big wind X 50 ppm kinetin CRMG nutrient 5 35 60 70 Artificial mixed soil Big wind O 50 ppm kinetin CRMG nutrient 0 0 5 30 Artificial mixed soil K-1 X 50 ppm kinetin water 5 30 60 80 Artificial mixed soil K-1 O 50 ppm kinetin water 0 0 15 35 Artificial mixed soil K-1 X 50 ppm kinetin CRMG nutrient 5 25 45 75 Artificial mixed soil K-1 O 50 ppm kinetin CRMG nutrient 0 0 5 15

As a result, it was confirmed that the treatments immersed in kinetin solution using soil type-3 soil showed the best growth.

Specifically, it was confirmed that there was no significant difference in growth depending on the seed cultivars.

Firstly, it was confirmed that the treated soil immersed in water had little or no growth of germination and seedling germ, and was very poor as less than 30%. On the other hand, in the case of immersion treatment with kinetin solution, germination rate was about 80%, and germination progressed in almost all seeds, and the level was about twice that of immersion in water.

In addition, coated seeds showed germination delayed by about 3 weeks compared to uncoated seeds.

In addition, soil type-1 or soil type-3 as a soil condition was found to be not suitable for growth because of low germination rate. The soil type-1 artificial soil has a low specific gravity and is an irrigation system in the growing chamber. When the water or CRMG nutrient is sprayed, the seeds float in the water and sink, causing the seed to move at the sowing point. And it was found to be somewhat inappropriate for growth. However, it was confirmed that soil type-3, which supplemented the disadvantages of artificial soil by mixing soil type-1 with rusty soil, provided an excellent environment for growth.

Finally, it was confirmed that there was no significant difference in growth effect when water or CRMG nutrient solution was used as an irrigation solution.

Taking all the above results into consideration, through the above Examples 1 and 2, the ginseng seeds were immersed in a GA solution at a concentration of 100 ppm for 24 hours; The seeds that had been immersed were cultured in a growth medium chamber maintained at 25 ° C. for 42 days in a mixed nutrient solution containing CRMG nutrient and 10 ppm GA solution diluted 100 times with the cultured ginseng cultured muscle (CRMG) To perform a decantering process; The seeds having been subjected to the decarburization treatment were placed at 10 캜 for 28 days for cold treatment; The seed treated with the low temperature treatment was immersed in 50 ppm of kinetin for 24 hours; The seeds thus dipped were mixed in an artificial mixed soil having a volume ratio of 1: 1 of artificial soil mixed with vermiculite: perlite: peat moss at a volume ratio of 3: 1: 1 It was confirmed that the method of growing by sowing is the optimal condition for promoting the opening of the ginseng seed and increasing the growth.

From the above description, it will be understood by those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention without departing from the scope of the present invention as defined by the appended claims.

Claims (12)

(a) immersing the ginseng seed in a solution of gibberellic acid (GA);
(b) cultivating the seed having been immersed in a mixed solution of CRMG (Cultured Roots of Mountain Ginseng) nutrient and GA solution;
(c) low-temperature treatment of the seeds having been subjected to deionization at 5 to 15 캜;
(d) immersing the seed having undergone the low temperature treatment in a kinetin solution; And
(e) the seeds in which the immersed seeds are contained in a volume ratio of vermiculite: perlite: peat moss of 2.5 to 3.5: 0.5 to 1.5: 0.5 to 1.5 in a volume ratio of 0.5 to 1.5: And seeding the mixed soil at a volume ratio of 0.5 to 1.5. 2. The method according to claim 1, wherein the immersion in steps (a) and (d) is carried out for 20 to 30 hours. 2. The method according to claim 1, wherein the GA solution of step (a) has a concentration of 90 to 110 ppm. 2. The method according to claim 1, wherein the GA solution of step (b) has a concentration of 5 to 15 ppm. The method according to claim 1, wherein said step (b) is carried out in an incubator. The growth method according to claim 5, wherein the incubator has a temperature of 20 to 30 ° C. 2. The method according to claim 1, wherein said step (b) is performed for 40 to 45 days. The method according to claim 1, wherein said step (c) is performed for 25 to 30 days. The growth method according to claim 1, wherein the kinetin solution of step (d) has a concentration of 40 to 60 ppm. delete delete delete

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