CN115104440B - Seedling raising method for promoting cutting rooting of agilawood branches - Google Patents

Seedling raising method for promoting cutting rooting of agilawood branches Download PDF

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CN115104440B
CN115104440B CN202210778245.1A CN202210778245A CN115104440B CN 115104440 B CN115104440 B CN 115104440B CN 202210778245 A CN202210778245 A CN 202210778245A CN 115104440 B CN115104440 B CN 115104440B
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cutting
agilawood
root
promoting
seedling
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CN115104440A (en
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范武波
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Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
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Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00—Vegetative propagation
    • A01G2/10—Vegetative propagation by means of cuttings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The invention provides a seedling raising method for promoting cutting rooting of agilawood branches, which comprises the following steps: (1) selecting spike strips; (2) Cutting the root of the cutting slip into an inclined plane incision, firstly putting the cutting slip into root promoting liquid for soaking, and transferring the cutting slip into base treatment liquid containing pyrazinamide and carboxymethyl cellulose for soaking; (3) Taking out cutting slips, cutting in a matrix, and spraying diluted basal treatment liquid twice after cutting for 3 d; (4) Spraying plant nutrient solution and diluted root promoting solution in combination for strengthening root and seedling 2 weeks after cutting, and obtaining agilawood cutting seedlings after the seedling grows to be more than or equal to 40cm, and planting in a field after the seedling leaves the nursery; the agilawood branch cutting seedling method can effectively improve the fresh state of agilawood cutting slips, remarkably accelerate the growth and development of agilawood root systems, and fully improve the quality of sprouting and root systems.

Description

Seedling raising method for promoting cutting rooting of agilawood branches
Technical Field
The invention relates to the technical field of agalloch eaglewood asexual propagation, in particular to a seedling raising method for promoting agalloch eaglewood branches to root by cutting.
Background
The eaglewood tree is mainly composed of different species such as eaglewood of Mallotus, eaglewood of Aquilaria of Rabdosia. The domestic agilawood is mainly planted by two species of aquilaria sinensis and agilawood of Yunnan. The Qilan agilawood, also called Jianan agilawood and Ganlan agilawood, is mostly distributed in southeast Asian countries such as Vietnam, malaysia, burma and Thailand, and is mainly introduced in regions such as Yunnan, guangdong, guangxi, fujian and Hainan. The method has the advantages of quick aroma formation, short aroma formation period, high yield, strong disease and pest resistance and the like. In the agilawood planting production process, aquilaria sinensis mainly adopts a seed propagation mode, and the seed propagation of the agilawood of the phoenix is difficult to maintain the excellent characters of the female parent, and the asexual propagation is realized. Compared with the growth of seeding seedlings, the cutting propagation is faster, can be used for quickly cultivating seedlings, shortens the growth period of agilawood, is beneficial to retaining the advantages of parent plants, but in the cutting propagation process, the rooting development of the agilawood is slower, the seedling root system is more fragile compared with the seeding seedlings propagated by seeds, and the problems of low survival rate and poor root quality of seedlings in the cutting propagation seedling stage are easy to occur during batch propagation of cutting bars, so that a seedling cultivation method capable of effectively promoting the rooting of the agilawood branches of the agilawood is sought, the asexual propagation technology of the agilawood of the schefflera is further improved, the propagation efficiency is improved, the propagation cost is reduced, and popularization and application of excellent varieties are promoted.
Disclosure of Invention
In view of the above, the invention provides a seedling raising method for promoting the cutting rooting of agilawood branches, which can effectively improve the fresh state of agilawood cutting slips, remarkably accelerate the growth and development of agilawood root systems and fully improve the quality of sprouting and root systems.
The technical scheme of the invention is realized as follows:
the invention provides a seedling raising method for promoting cutting rooting of agilawood branches, which comprises the following steps:
step 1: selecting spike: selecting annual healthy branches of agilawood with the length of 15-18cm from 3-5 annual healthy parent plants as cutting slips, reserving 2-3 bud eyes outside 6-8cm from the base of the cutting slips, and reserving 3-4 leaves at the top;
step 2: root promotion treatment: placing the base of the cutting into root promoting liquid for soaking for 2-3h after disinfecting, and transferring into base treatment liquid containing pyrazinamide and carboxymethyl cellulose for soaking for 8-10min;
step 3: cutting: taking out the root-promoting treated cutting slips, cutting in a matrix with a cutting depth of 3.5-4.5cm, compacting the jacks, spraying water to penetrate the matrix, and spraying 20-30 times diluted base treatment liquid twice after cutting for 3d, wherein each time is 5d apart;
step 4: root and seedling strengthening: and (3) spraying the plant nutrient solution and the diluted root promoting solution in a combined manner for strengthening roots and seedlings 2 weeks after cutting, spraying the plant nutrient solution once every 3-4d, spraying the plant nutrient solution once every week, and carrying out periodic disinfection and watering, and obtaining the agilawood cutting seedlings after the seedlings grow to be more than or equal to 40cm, and carrying out nursery-out and field-entering cultivation.
Further described, the root promoting liquid is formed by mixing 0.5-0.8 part (1-2 part) and 20-30 parts (1000 parts) of triiodobenzoic acid, cinnamic acid, ethephon and water according to the mass ratio, and the root promoting liquid is prepared by combining the cinnamic acid and the ethephon with low concentration, and is used as the root promoting liquid for the cutting rooting of agilawood, and the root promoting liquid is applied to the agilawood before and after the cutting in different concentrations, so that the rooting rate of the agilawood cutting is remarkably improved.
Further, the base treatment liquid comprises pyrazinamide, carboxymethyl cellulose and water in a mass ratio of (5-8) to (1-3) to (15-16), and the pyrazinamide and the carboxymethyl cellulose in a certain proportion are used as the base treatment liquid, so that the fresh state of agilawood cutting shoots can be prolonged, the loss of moisture of the shoots is slowed down, the rot of the shoot bases in the early stage of cutting is reduced, and the survival rate and rooting rate are improved.
Further stated, the substrate comprises an upper substrate and a lower substrate with a mass ratio of 3:1, wherein the upper substrate is formed by mixing loess, coconut husk, biogas residues and vermiculite with a mass ratio of 2:2:1:1; the lower matrix is formed by mixing laterite, peat and perlite in a ratio of 2:2:1.
Further, the plant nutrient solution is prepared by mixing potassium dihydrogen phosphate, magnesium sulfate, calcium nitrate, urea and water according to a mass ratio of 2:1:1:3:20.
Further, in the step 3, the sunshade rate is controlled to be 75-85% in the first 4 weeks after cutting, and the sunshade rate is controlled to be 55-65% after cutting for 1 month, and the cutting temperature is kept to be 25-30 ℃, and the relative humidity is more than or equal to 75%.
Further, in the step 3, the spraying amount of the diluted base treatment liquid is 80-100 mL/plant.
Further described, the root and seedling strengthening is that the mass ratio of the plant nutrient solution and the diluted root promoting solution is (5-8): 1-3, and the diluted root promoting solution is 200 times of the root promoting solution diluted by water.
Further, in the step 4, the spraying amount of the plant nutrient solution and the diluted root promoting solution sprayed in combination is mainly used for spraying the matrix in the range of 6-8cm away from the cutting seedlings 2 weeks after cutting, and the spraying amount of the plant nutrient solution is mainly used for spraying the matrix in the range of 8-10cm away from the cutting seedlings 2 weeks later.
Further, in the step 2, the disinfection treatment adopts 0.06-0.1% potassium permanganate solution to clean the bevel incision of the base part of the cutting slip for 1-2 times.
Further illustratively, the periodic disinfection and watering is: the matrix is sprayed by adopting a potassium permanganate solution with the mass percentage of 0.1-0.3% and the carbendazim 1000 times at intervals of 3-4d, the chlorothalonil with the liquid 1000 times is sprayed on the circumferential spike strips once every 3 weeks after cuttage, watering is carried out every week, and the matrix is sprayed until no water is accumulated.
Compared with the prior art, the invention has the beneficial effects that:
(1) According to the invention, after the agilawood cutting is treated by the root promoting liquid, the base soaking treatment is carried out by adopting the base treating liquid containing pyrazinamide and carboxymethyl cellulose, and the diluted low-concentration base treating liquid is further sprayed in the early stage after cutting, so that the fresh state of the agilawood cutting can be effectively improved before the root system is not formed and does not recover to grow, the moisture in the cutting is maintained, the rotten condition of the base of the cutting is less, the growth and development of the root system are obviously promoted, and the survival rate of agilawood cutting is improved.
(2) According to the invention, the plant nutrient solution and the diluted root promoting solution are adopted for combined spraying 2 weeks after cutting, so that the root system development of agilawood cutting slips is obviously improved, the generation of lateral roots is promoted, the rooting quantity is increased, the agilawood cutting slips quickly form stable root systems, the rooting rate is improved, the absorption of nutrient substances is enhanced, the nutrition distribution is regulated, the growth and development of cutting seedlings are accelerated, and the emergence and root system growth quality of agilawood cutting seedlings is improved.
Detailed Description
In order to better understand the technical content of the present invention, the following provides specific examples to further illustrate the present invention.
The experimental methods used in the embodiment of the invention are conventional methods unless otherwise specified.
Materials, reagents, and the like used in the examples of the present invention are commercially available unless otherwise specified.
Example 1-seedling raising method for cutting rooting of agilawood branches, comprising the following steps:
(1) Selecting annual robust branches of agilawood with the length of 15-18cm from 3 annual robust mother plants as cutting slips, reserving 2-3 bud eyes outside 6-8cm from the base of the cutting slips, and reserving 3-4 leaves at the top;
(2) Mixing triiodobenzoic acid (98% triiodotoluic acid powder), cinnamic acid (99% triiodotoluic acid powder), ethephon (industrial grade purity 40% ethephon needle crystal) and water according to a mass ratio of 0.5:1:20:1000 to prepare root promoting liquid, and mixing pyrazinamide, carboxymethyl cellulose and water according to a mass ratio of 5:1:15 to prepare base treatment liquid; the upper part of the cutting slip is provided with a plane incision, the base part is provided with an inclined plane incision, after the cutting slip is disinfected by adopting 0.06 percent potassium permanganate solution, the base part of the cutting slip is firstly put into root promoting liquid for soaking for 2 hours, and then is transferred into base treatment liquid containing pyrazinamide and carboxymethyl cellulose for soaking for 8 minutes;
(3) An upper substrate and a lower substrate with a mass ratio of 3:1 are adopted to form a cuttage substrate, and the upper substrate is formed by mixing loess, coconut coir, biogas residues and vermiculite according to a mass ratio of 2:2:1:1; the lower matrix is formed by mixing laterite, peat and perlite in a ratio of 2:2:1;
taking out the root-promoting treated cutting slips, cutting the cutting slips in a matrix, wherein the cutting depth is 3.5-4.5cm, compacting insertion holes, spraying water to penetrate the matrix, cutting for 3d, spraying a base treatment liquid diluted 20 times by water, and repeatedly spraying once after 5d intervals, wherein the spraying amount is 80 mL/plant; the sunshade rate is controlled to be 75-85% in the first 4 weeks of cuttage, then the sunshade rate is adjusted to be 55-65%, and the cuttage temperature is kept to be 25-30 ℃ and the relative humidity is more than or equal to 75%;
(4) Spraying a plant nutrient solution and a diluted root promoting solution in a mass ratio of 5:1 in a combined manner for strengthening roots and seedlings 2 weeks after cutting, wherein the plant nutrient solution is formed by mixing potassium dihydrogen phosphate, magnesium sulfate, calcium nitrate, urea and water in a mass ratio of 2:1:1:3:20; the diluted root promoting liquid is the root promoting liquid diluted by 200 times by water; the spraying amount is mainly that the substrate which is wetted within a range of 6-8cm away from the cutting seedlings is sprayed once every 3 days; and then spraying plant nutrient solution once a week, taking a matrix which is wetted by the cutting seedling within a range of 8-10cm as a main material, and periodically sterilizing and watering, and obtaining the agilawood cutting seedling after the seedling grows to be more than or equal to 40cm, and taking the agilawood cutting seedling out of the nursery and planting the agilawood cutting seedling in the field.
Example 2-seedling raising method for cutting rooting of agilawood branches, comprising the following steps:
(1) Selecting annual robust branches of agilawood with the length of 15-18cm from a healthy parent plant of 5 years as cutting slips, reserving 2-3 bud eyes outside 6-8cm from the base of the cutting slips, and reserving 3-4 leaves at the top;
(2) Mixing tri-iodo benzoic acid, cinnamic acid, ethephon and water according to a mass ratio of 0.8:2:30:1000 to prepare a root promoting liquid, and mixing pyrazinamide, carboxymethyl cellulose and water according to a mass ratio of 8:3:16 to prepare a base treatment liquid; the upper part of the cutting slip is provided with a plane incision, the base part is provided with an inclined plane incision, after the sterilization by adopting 0.1 percent potassium permanganate solution, the base part of the cutting slip is firstly put into root promoting liquid for soaking for 3 hours, and then is transferred into base treatment liquid containing pyrazinamide and carboxymethyl cellulose for soaking for 10 minutes;
(3) An upper substrate and a lower substrate with a mass ratio of 3:1 are adopted to form a cuttage substrate, and the upper substrate is formed by mixing loess, coconut coir, biogas residues and vermiculite according to a mass ratio of 2:2:1:1; the lower matrix is formed by mixing laterite, peat and perlite in a ratio of 2:2:1;
taking out the root-promoting treated cutting slips, cutting the cutting slips in a matrix, wherein the cutting depth is 3.5-4.5cm, compacting insertion holes, spraying water to penetrate the matrix, cutting for 3d, spraying a base treatment liquid diluted by 30 times of water, and repeating spraying once after 5d, wherein the spraying amount is 100 mL/plant; the sunshade rate is controlled to be 75-85% in the first 4 weeks of cuttage, then the sunshade rate is adjusted to be 55-65%, and the cuttage temperature is kept to be 25-30 ℃ and the relative humidity is more than or equal to 75%;
(4) Spraying a plant nutrient solution and a diluted root promoting solution in a mass ratio of 8:3 in a combined manner for strengthening roots and seedlings 2 weeks after cutting, wherein the plant nutrient solution is formed by mixing potassium dihydrogen phosphate, magnesium sulfate, calcium nitrate, urea and water in a mass ratio of 2:1:1:3:20; the diluted root promoting liquid is the root promoting liquid diluted by 200 times by water; the spraying amount is mainly that the substrate which is wetted within a range of 6-8cm away from the cutting seedlings is sprayed once every 3 days; and then spraying plant nutrient solution once a week, taking a matrix which is wetted by the cutting seedling within a range of 8-10cm as a main material, and periodically sterilizing and watering, and obtaining the agilawood cutting seedling after the seedling grows to be more than or equal to 40cm, and taking the agilawood cutting seedling out of the nursery and planting the agilawood cutting seedling in the field.
Example 3-seedling raising method for cutting rooting of agilawood branches, comprising the following steps:
(1) Selecting annual robust branches of agilawood with the length of 15-16cm from 4 annual robust mother plants as cutting slips, reserving 2-3 bud eyes outside 6-7cm from the base of the cutting slips, and reserving 3-4 leaves at the top;
(2) Mixing tri-iodo benzoic acid, cinnamic acid, ethephon and water according to a mass ratio of 0.6:1.5:25:1000 to prepare a root promoting liquid, and mixing pyrazinamide, carboxymethyl cellulose and water according to a mass ratio of 6:2:16 to prepare a base treatment liquid; the upper part of the cutting slip is provided with a plane incision, the base part is provided with an inclined plane incision, after the cutting slip is disinfected by adopting 0.08 percent potassium permanganate solution, the base part of the cutting slip is firstly put into root promoting liquid for soaking for 3 hours, and then is transferred into base treatment liquid containing pyrazinamide and carboxymethyl cellulose for soaking for 10 minutes;
(3) An upper substrate and a lower substrate with a mass ratio of 3:1 are adopted to form a cuttage substrate, and the upper substrate is formed by mixing loess, coconut coir, biogas residues and vermiculite according to a mass ratio of 2:2:1:1; the lower matrix is formed by mixing laterite, peat and perlite in a ratio of 2:2:1;
taking out the root-promoting treated cutting slips, cutting the cutting slips in a matrix, wherein the cutting depth is 3.5-4.5cm, compacting insertion holes, spraying water to penetrate the matrix, cutting for 3d, spraying a base treatment liquid diluted by 25 times of water, and repeatedly spraying once after 5d, wherein the spraying amount is 90 mL/plant; the sunshade rate is controlled to be 75-85% in the first 4 weeks of cuttage, then the sunshade rate is adjusted to be 55-65%, and the cuttage temperature is kept to be 25-30 ℃ and the relative humidity is more than or equal to 75%;
(4) Spraying a plant nutrient solution and a diluted root promoting solution in a mass ratio of 6:2 in a combined manner for strengthening roots and seedlings 2 weeks after cutting, wherein the plant nutrient solution is formed by mixing potassium dihydrogen phosphate, magnesium sulfate, calcium nitrate, urea and water in a mass ratio of 2:1:1:3:20; the diluted root promoting liquid is the root promoting liquid diluted by 200 times by water; the spraying amount is mainly that the substrate which is wetted within a range of 6-8cm away from the cutting seedlings is sprayed once every 3 days; and then spraying plant nutrient solution once a week, taking a matrix which is wetted by the cutting seedling within a range of 8-10cm as a main material, and periodically sterilizing and watering, and obtaining the agilawood cutting seedling after the seedling grows to be more than or equal to 40cm, and taking the agilawood cutting seedling out of the nursery and planting the agilawood cutting seedling in the field.
Example 4
The seedling raising method for cutting and rooting of agilawood branches in embodiment 3, wherein in step 3, triiodobenzoic acid, cinnamic acid, ethephon and water are mixed according to a mass ratio of 2:1.5:25:1000 to be used as root promoting liquid, the root promoting liquid is diluted by 200 times to be used as diluted root promoting liquid, and the diluted root promoting liquid is used for being combined and sprayed with plant nutrient solution in the first 2 weeks after cutting, and the spraying conditions are the same as those in embodiment 3.
Comparative example 1
According to the seedling raising method for cutting rooting of agilawood branches in embodiment 3, in the step 2, the base of the cutting is not soaked by adopting base treatment liquid, and in the step 3, after the 3d of cutting, the diluted base treatment liquid is not sprayed on the cutting.
Comparative example 2
In step 3, the undiluted basal treatment solution is sprayed to the cutting branches at the 3 rd day of cutting, and after 5 days, the spraying is repeated once, and the other conditions are the same as in example 3.
Comparative example 3
In step 3, the base treatment liquid diluted 50 times by water is sprayed to the cutting slip at the 3 rd day of cutting, and after 5 days of interval, the spraying is repeated once, and the other conditions are the same as in example 3.
Comparative example 4
In the seedling raising method for cutting rooting of agilawood branches in embodiment 3, in the step 4, the diluted root promoting liquid and the plant nutrient solution are not adopted for combined spraying, and the spraying amount of the plant nutrient solution is mainly used for spraying a matrix in a range of 6-8cm away from cutting seedlings in the first 2 weeks after cutting, and is sprayed once every 3 days; then spraying once a week, taking the matrix which is wetted by 8-10cm away from the cutting seedling as a main part, and carrying out periodic disinfection and watering, wherein the other conditions are the same as those of the example 3.
Example 5 planting experiment
Selecting a strong agilawood mother plant of the same batch growth of a test nursery, selecting annual strong agilawood branches with the length of 15-16cm on the mother plant as cutting branches, reserving 2-3 bud eyes outside 6-7cm away from the base of the cutting branches, reserving 3-4 leaves on the top, randomly setting 6 treatments, and repeating each treatment for 20 plants for 3 times according to the agilawood cutting seedling raising methods of the embodiments 3 and 4 and the comparative examples 1-4.
(1) After cutting and raising the agilawood cutting slips for 6 months, the growth condition of each processed agilawood cutting slip is investigated, the rooting position, the rooting number and the longest root length of the cutting slips are recorded, and the rooting rate and the survival rate are calculated, and the result is shown in the following table 1;
( Average root number = total number of roots per plant number of all plants; rooting rate = number of plants rooted/total number of plants x 100%; survival rate = number of surviving plants/total number of cutting plants x 100% )
TABLE 1 growth of different treated eaglewood cutting seedlings
As can be seen from the table, the average rooting rate of the treatment 2 is lower than that of the treatment 1, and the length of the longest root is shorter, which indicates that the concentration of the triiodobenzoic acid is controlled, and the triiodobenzoic acid is combined with cinnamic acid and ethephon under the lower concentration, so that the rooting rate of agilawood in deep cutting can be effectively improved, the development speed of the root system is accelerated, and the length of the root is improved. The quality of the cutting growth of the agilawood in the treatment 3 is the lowest, the survival rate and the rooting rate are low, the root system development is slow, and the rooting effects of the treatments 4 and 5 are lower than that of the treatment 1, which shows that the treatment of the base soaking by adopting the treatment liquid containing pyrazinamide and carboxymethyl cellulose as the base treatment liquid and the spraying of the diluted low-concentration base treatment liquid in the early stage after the cutting are beneficial to promoting the growth and development of the root system. In the treatment 6, the diluted root promoting liquid and the plant nutrient liquid are not adopted for combined spraying, and the rooting amount and the rooting rate are reduced, which shows that the combined spraying of the plant nutrient liquid and the diluted root promoting liquid is favorable for further promoting the combined spraying of the plant nutrient liquid and the diluted root promoting liquid, increasing the rooting amount and accelerating the growth and development of the cutting seedlings.
(2) Measurement of polyphenol oxidase (PPO) in rooting of treated cuttings by catechol colorimetric method of Li Huanxiu et al, samples were taken from cuttings at 10 th, 20 th and 30 th days after cutting, and polyphenol oxidase (PPO) activity was measured, and the results are shown in table 2 below.
TABLE 2 determination of polyphenol oxidase activity of different treated agilawood cutting seedlings
As can be seen from the table, the polyphenol oxidase in the cutting slips can regulate the growth metabolism of the cutting slips and promote the development of root systems, and the PPO activity of the cutting slips after 10d in the early stage of cutting in treatments 3 and 5 is obviously lower than that of treatment 3, which indicates that the invention adopts the base treatment liquid to carry out base soaking treatment before cutting, and the dilute low-concentration base treatment liquid is sprayed in the early stage of cutting, thereby being beneficial to keeping the fresh state of the cutting slips and promoting the rapid recovery and generation of the root systems of the cutting slips. And along with the extension of the cutting time, the PPO activity of agilawood cutting slips in treatments 1, 2 and 4 is in an ascending trend, and the PPO activity in treatment 3 is slow in growth rate, which shows that the base treatment liquid is beneficial to promoting the growth and development of root systems. After 30d of cutting, the polyphenol oxidase (PPO) activity of the cutting slips of the treatment 3 and the treatment 6 is lower, and the treatment 4 and the treatment 5 are the treatment of the base treatment liquid at the early stage of cutting, and the combined spraying of the plant nutrient solution and the diluted root promoting liquid is adopted 2 weeks before cutting, so that the root system development of the agilawood cutting slips is fully improved, the agilawood cutting slips form stable root systems rapidly, and the quality of emergence and root systems is improved.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.

Claims (5)

1. A seedling raising method for promoting agilawood branch cutting rooting is characterized by comprising the following steps of: the method comprises the following steps:
step 1: selecting spike: selecting annual healthy branches of agilawood with the length of 15-18cm from 3-5 annual healthy parent plants as cutting slips, reserving 2-3 bud eyes outside 8cm from the base of the cutting slips, and reserving 3-4 leaves at the top;
step 2: root promotion treatment: the upper part of the cutting slip is cut in a plane, the base part of the cutting slip is cut in an inclined plane, after disinfection, the base part of the cutting slip is firstly placed into root promoting liquid to be soaked for 2-3 hours, the root promoting liquid is prepared by mixing triiodobenzoic acid, cinnamic acid, ethephon and water according to the mass ratio of (0.5-0.8) (1-2) (20-30) (1000), and then the mixture is transferred into base treatment liquid containing pyrazinamide and carboxymethyl cellulose to be soaked for 8-10 minutes;
step 3: cutting: taking out the root-promoting treated cutting slips, cutting the cutting slips into a matrix, compacting the inserting holes, and leaching the matrix by water, wherein the matrix comprises an upper matrix and a lower matrix with a mass ratio of 3:1, and the upper matrix is formed by mixing loess, coconut husk, biogas residues and vermiculite according to a mass ratio of 2:2:1:1; the lower substrate is formed by mixing laterite, peat and perlite in a ratio of 2:2:1, 20-30 times diluted base treatment liquid is sprayed twice after the 3 rd day of cuttage, and the base treatment liquid comprises pyrazinamide, carboxymethyl cellulose and water in a mass ratio of (5-8) to (1-3) to (15-16), wherein each time is separated by 5d;
step 4: root and seedling strengthening: and (1-3) the mass ratio of the plant nutrient solution to the diluted root promoting solution is (5-8), the diluted root promoting solution is the root promoting solution diluted by 200 times by water, the plant nutrient solution is formed by mixing potassium dihydrogen phosphate, magnesium sulfate, calcium nitrate, urea and water according to the mass ratio of 2:1:1:3:20, the plant nutrient solution is sprayed once every 3-4d, the plant nutrient solution is sprayed once every week, the plant nutrient solution is disinfected and watered regularly, and agilawood cutting seedlings are obtained after the seedling grows to be more than or equal to 40cm, and the agilawood cutting seedlings are cultivated out of a nursery.
2. The seedling raising method for promoting cutting rooting of agilawood branches according to claim 1, wherein the seedling raising method is characterized by comprising the following steps of: in the step 3, the sunshade rate is controlled to be 75-85% in the first 4 weeks after cutting, the sunshade rate is controlled to be 55-65% after cutting for 1 month, and the cutting temperature is kept to be 25-30 ℃ and the relative humidity is more than or equal to 75%.
3. The seedling raising method for promoting cutting rooting of agilawood branches according to claim 1, wherein the seedling raising method is characterized by comprising the following steps of: in the step 3, the spraying amount of the diluted base treatment liquid is 80-100 mL/plant.
4. The seedling raising method for promoting cutting rooting of agilawood branches according to claim 1, wherein the seedling raising method is characterized by comprising the following steps of: in the step 4, the spraying amount of the plant nutrient solution and the diluted root promoting solution is mainly used for spraying the matrix in the range of 6-8cm away from the cutting seedlings after 2 weeks after cutting, and the spraying amount of the plant nutrient solution is mainly used for spraying the matrix in the range of 8-10cm away from the cutting seedlings after 2 weeks.
5. The seedling raising method for promoting cutting rooting of agilawood branches according to claim 1, wherein the seedling raising method is characterized by comprising the following steps of: in the step 2, the disinfection treatment adopts 0.06-0.1% potassium permanganate solution to clean the bevel incision of the base part of the cutting slip for 1-2 times.
CN202210778245.1A 2022-06-30 2022-06-30 Seedling raising method for promoting cutting rooting of agilawood branches Active CN115104440B (en)

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