CN101878733B - Method for promoting germination of guineagrass seed - Google Patents
Method for promoting germination of guineagrass seed Download PDFInfo
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- CN101878733B CN101878733B CN2010102354318A CN201010235431A CN101878733B CN 101878733 B CN101878733 B CN 101878733B CN 2010102354318 A CN2010102354318 A CN 2010102354318A CN 201010235431 A CN201010235431 A CN 201010235431A CN 101878733 B CN101878733 B CN 101878733B
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Abstract
The invention discloses a method for promoting germination of guineagrass seeds, which comprises the following steps: processing the guineagrass seeds by a method of water immersion at the low temperature to obtain the processed guinegrass seeds; and inserting the processed guineagrass seeds into a solid matrix to cultivate so as to obtain the germinated seeds. The method for promoting germination of the guineagrass seeds provided by the invention is simple and easy to implement, is free from complex operation, can quickly effectively improve the germination rate of the guineagrass seeds, can be applied to the production to solve the problem that the guineagrass has low germination efficiency due to the 'three lower' during the production, and has a great significance to the construction and planting of the artificial guineagrass grassland.
Description
Technical field
The present invention relates to a kind of method that promotes germination of guineagrass seed.
Background technology
Sheep's hay (Leymus chinensis) is under the jurisdiction of Monocotyledonae, grass family, Agrostidoideae, annual bluegrass system, barley family, wild wheat subtribe, leymus plant, is one of important grassland constructive species of grassland region east, Eurasia.As a kind of important herbage resource, sheep's hay has output height, strong stress resistance, animal good palatability, plurality of advantages such as nutritious; Simultaneously, sheep's hay also has stronger adaptability to the different ecological environment, is significant with improvement salination soil to improving the northern China ecology of grassland.But sheep's hay self exists " three is low " problem, i.e. heading rate is low, ripening rate is low, seed germination rate is low, has limited seed production and the development of man-made grasslands of sheep's hay.Therefore, improving the germination of guineagrass seed rate has great importance.
Summary of the invention
The purpose of this invention is to provide a kind of method that makes germination of guineagrass seed, comprise the steps: to handle Leymus chinensis seeds, the Leymus chinensis seeds after obtaining handling with low temperature with the method that the water logging bubble combines earlier; Leymus chinensis seeds after the said processing is broadcast into solid matrix, cultivated, the seed that obtains sprouting.
Said solid matrix is the mixture of nutrition soil and vermiculite, and the volume ratio of nutrition soil and vermiculite is 2: 1.
Said low temperature is that said Leymus chinensis seeds is soaked in water with the method that the water logging bubble combines, and is positioned over simultaneously under 4 ℃ of conditions.
The time of said processing is 1-6 days, is specially 1 day or 4 days or 6 days.
The periodicity of illumination of said cultivation is illumination/12 a hour dark 12 hours every days.
Temperature when the temperature of said cultivation is illumination is that 28 ℃ of temperature when dark are 16 ℃.
Said Leymus chinensis seeds is the seed of the sheep's hay of following kind or strain: sheep's hay strain S1-2, S2-2, S3-2, S4-3,1602 or the sheep's hay kind is lucky gives birth to No. one.
The method of quick raising germination of guineagrass seed rate provided by the present invention is simple, need not complex operations, can improve the germination rate and the sprouting speed of Leymus chinensis seeds quickly and efficiently.Can the method be applied to produce, thereby solve low problem of germination rate in " the three low problems " that sheep's hay runs in process of production, the planting of sheep's hay sown pasture is had great importance.
Description of drawings
The upgrowth situation figure of Fig. 1 for 6 kinds of different Leymus chinensis seeds are handled with 4 kinds of different disposal modes respectively.
Embodiment
Employed experimental technique is conventional method like no specified otherwise among the following embodiment.
Used material, reagent etc. like no specified otherwise, all can obtain from commercial sources among the following embodiment.
Embodiment 1, water at low temperature immersion treatment improve the germination of guineagrass seed rate.
Method I
One, gathers Leymus chinensis seeds
Test used Leymus chinensis seeds and be respectively following strain: S1-2, S2-2, S3-2, S4-3 and 1602 and the seed of kind Q7 (lucky giving birth to No. one) (purchase) from Jisheng Improved Variety Station of Aneurolepidium Chinense, Jilin Province.
Get the mature seed of above-mentioned 6 kinds of different sheep's hay germplasms, the seed of therefrom selecting full seed, big or small basically identical respectively carries out following processing.
Two, before the sowing Leymus chinensis seeds is handled
Above-mentioned 6 kinds of different Leymus chinensis seeds selecting are handled with 4 kinds of different disposal modes respectively: 1d under 26 ℃ of freeze-day with constant temperature conditions of control treatment (hereinafter to be referred as the constant temperature H that desiccates); 1d under 4 ℃ of low temperature drying conditions (hereinafter to be referred as the low temperature D that desiccates); 26 ℃ of thermostatted waters soak 1d (HS); 4 ℃ of water at low temperature are soaked 1d (DS).HS group and DS group, every seed that water logging is steeped at a distance from 8h mixes and shakes once during the processing, and seed is fully absorbed water.
Three, the Leymus chinensis seeds after handling is sowed cultivation
After accomplishing above-mentioned 4 kinds of processing modes; Get 6 kinds of different sheep's hay germplasm S1-2 after the above-mentioned processing, S2-2, S3-2, S4-3,1602 and each 50 full seed of seed of Q7 respectively; Be seeded in respectively in the diameter 9cm flowerpot, about the earthing 5mm of top layer, the seed sprouting medium is a nutrition soil and (nutrition soil is 2: 1 with the vermiculite volume ratio to the mixture of vermiculite; Nutrition soil is available from the birds and flowers fish market), the mixture of nutrition soil and vermiculite is watered the sowing of permeable back.
After planting 6 kinds of different Leymus chinensis seeds are placed in the greenhouse cultivate, periodicity of illumination is 12 hours illumination/12 hour dark, cultivation temperature be illumination condition following 28 ℃ with following 16 ℃ of dark condition, water once every three days.
Four, measure the germination rate of Leymus chinensis seeds
To seed after planting, add up germination rate every day one time.The data SPSS15.0 carries out statistical analysis, grows and after 30 days germination rate is carried out one-way analysis of variance, and analysis result is seen table 1.
The average germination rate of Leymus chinensis seeds under the table 1 different disposal condition
| Processing mode/numbering | S1-2 | S2-2 | S3-2 | S4-3 | 1602 | Q7 |
| 26 ℃ of constant temperature desiccate (H) | 64.67 | 44.00 | 45.67 | 76.33 | 78.00 | 37.67 |
| 4 ℃ of low temperature desiccate (D) | 57.67 | 53.00 | 41.33 | 71.67 | 81.67 | 37.00 |
| 26 ℃ of thermostatted waters soak (HS) | 58.67 | 69.67 | 52.67 | 87.67 | 83.00 | 49.33 |
| 4 ℃ of water at low temperature are soaked (DS) | 78.00* | 84.33* | 72.67* | 87.33* | 91.67* | 67.67* |
Annotate: * representes that 4 ℃ of water at low temperature are soaked (DS) and above-mentioned other three kinds of processing modes compare significant difference under 0.05 level.
The seed that the seed that grows seedling is decided to be sprouting.The computing formula of germination rate is (seed number of sprouting)/50 * 100%.
3 repetitions are established in experiment, and the result takes the mean.
The result shows; There is significant difference (P＜0.05) in 6 kinds of different Leymus chinensis seeds germination rate under 26 ℃ of constant temperature of control treatment desiccate the 1d condition, and wherein 1602 germination rates are higher reaches 78%, and the Q7 germination rate is 37.67%; Explain that its germination rate there are differences because the hereditary capacity of sheep's hay self influences.Soak 1d and the 4 ℃ of low temperature 1d that desiccates and compare with desiccate 1d, 26 ℃ of thermostatted waters of 26 ℃ of constant temperature; The germination rate that 4 ℃ of water at low temperature are soaked the following 6 kinds of sheep's hays of 1d condition be significantly increased (P＜0.05); Wherein be up to 1602, germination rate can reach 91.67%, and the Q7 germination rate is 67.67%.The water at low temperature immersion treatment is compared with other treatment conditions, can reach higher germination rate fast, thereby can improve the germination rate of Leymus chinensis seeds fast.
Sprouting situation (Fig. 1) by Leymus chinensis seeds under the different disposal condition after planting can find out that also the seed germination situation of water at low temperature immersion treatment obviously is better than the seed germination situation of other treatment conditions.
Method II
One, gathers Leymus chinensis seeds
I is identical with method
Two, before the sowing Leymus chinensis seeds is handled
Above-mentioned 6 kinds of different Leymus chinensis seeds selecting are handled with 4 kinds of different disposal modes respectively: 4d under 26 ℃ of freeze-day with constant temperature conditions of control treatment (hereinafter to be referred as the constant temperature H that desiccates); 4d under 4 ℃ of low temperature drying conditions (hereinafter to be referred as the low temperature D that desiccates); 26 ℃ of thermostatted waters soak 4d (HS); 4 ℃ of water at low temperature are soaked 4d (DS).HS group and DS group, every seed that water logging is steeped at a distance from 8h mixes and shakes once during the processing, and seed is fully absorbed water.
Three, the Leymus chinensis seeds after handling is sowed cultivation
I is identical with method
Four, measure the germination rate of Leymus chinensis seeds
The result who measures result and method I does not have significant difference.
Method III
One, gathers Leymus chinensis seeds
I is identical with method
Two, before the sowing Leymus chinensis seeds is handled
Above-mentioned 6 kinds of different Leymus chinensis seeds selecting are handled with 4 kinds of different disposal modes respectively: 6d under 26 ℃ of freeze-day with constant temperature conditions of control treatment (hereinafter to be referred as the constant temperature H that desiccates); 6d under 4 ℃ of low temperature drying conditions (hereinafter to be referred as the low temperature D that desiccates); 26 ℃ of thermostatted waters soak 6d (HS); 4 ℃ of water at low temperature are soaked 6d (DS).HS group and DS group, every seed that water logging is steeped at a distance from 8h mixes and shakes once during the processing, and seed is fully absorbed water.
Three, the Leymus chinensis seeds after handling is sowed cultivation
I is identical with method
Four, measure the germination rate of Leymus chinensis seeds
The result who measures result and method I does not have significant difference.
Claims (4)
1. a method that makes germination of guineagrass seed comprises the steps: to handle Leymus chinensis seeds, the Leymus chinensis seeds after obtaining handling with low temperature with the method that the water logging bubble combines earlier; Leymus chinensis seeds after the said processing is broadcast into solid matrix, cultivated, the seed that obtains sprouting; Said low temperature is that said Leymus chinensis seeds is soaked in water with the method that the water logging bubble combines, and is positioned over simultaneously under 4 ℃ of conditions; The time of said processing is 1 day.
2. method according to claim 1 is characterized in that: the periodicity of illumination of said cultivation is illumination/12 a hour dark 12 hours every days.
3. method according to claim 1 and 2 is characterized in that: the temperature the when temperature of said cultivation is illumination is that 28 ℃ of temperature when dark are 16 ℃.
4. method according to claim 1 and 2 is characterized in that: said sheep's hay is lucky living No. of sheep's hay kind.
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| CN102668766A (en) * | 2012-05-10 | 2012-09-19 | 黑龙江省科学院高技术研究院 | Germination method of Leymus chinensis seeds |
| CN107840871B (en) * | 2016-09-18 | 2020-05-19 | 中国科学院植物研究所 | Seed development related protein LcbHLH92, and coding gene and application thereof |
| CN106717265A (en) * | 2016-12-02 | 2017-05-31 | 黑龙江省农业科学院草业研究所 | A kind of method that Leymus chinensis seeds are efficiently sprouted |
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| CN101199250B (en) * | 2006-12-14 | 2012-11-21 | 中国科学院东北地理与农业生态研究所 | Leymus chinensis low-temperature seed presoaking and temperature changing combination germinating method |
| CN101507394B (en) * | 2009-04-03 | 2011-04-20 | 中国科学院地理科学与资源研究所 | Chinese wild rye direct seeding method on saline-alkali land |
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Non-Patent Citations (2)
| Title |
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| 易津.羊草种子的休眠生理及提高发芽率的研究.《中国草地》.1994,(第6期),第1-6页. * |
| 马红媛等.提高羊草种子发芽率方法研究进展.《中国草地》.2005,第27卷(第4期),第64-68页. * |
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