CN106417282A - Paecilomyces fumosoroseus granules and preparing method thereof - Google Patents

Paecilomyces fumosoroseus granules and preparing method thereof Download PDF

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Publication number
CN106417282A
CN106417282A CN201610838326.0A CN201610838326A CN106417282A CN 106417282 A CN106417282 A CN 106417282A CN 201610838326 A CN201610838326 A CN 201610838326A CN 106417282 A CN106417282 A CN 106417282A
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China
Prior art keywords
ventilation
paecilomyces fumosoroseus
granule
parts
paecilomyces
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610838326.0A
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Chinese (zh)
Inventor
吴俊杰
李肖宇
曾升华
石仕福
梁小文
石峥
罗建辉
李英武
罗双燕
王根豪
严艺波
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Jiangxi Tianxiang General Aviation Co Ltd
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Jiangxi Tianxiang General Aviation Co Ltd
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Priority to CN201610838326.0A priority Critical patent/CN106417282A/en
Publication of CN106417282A publication Critical patent/CN106417282A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12Powders or granules
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/08Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom

Abstract

The invention provides paecilomyces fumosoroseus granules. The paecilomyces fumosoroseus granules are prepared from 5-15 parts of medicine active components, 10-25 parts of an additive, 50-90 parts of medicine carriers and 15-25 parts of water, wherein the medicine active components are paecilomyces fumosoroseus, and the paecilomyces fumosoroseus is selected from the culture with the biological collecting number is CGMCC No.7993. The paecilomyces fumosoroseus granules are efficient, low in toxicity, stable in pest killing effect, safe in people and livestock and plants and high in safety. The experimental result shows that the highest pest controlling effect of the paecilomyces fumosoroseus can be 88% or above.

Description

A kind of paecilomyces fumosoroseus granule and preparation method thereof
Technical field
The present invention relates to technical field of pesticide, more particularly to a kind of paecilomyces fumosoroseus granule and preparation method thereof.
Background technology
China is large agricultural country, and agricultural is the basis of national economy, is the source of the basic necessities of life of the mankind, survives originally, therefore agriculture The state of development of industry directly affects the global development of national economy.But in agricultural production process, insect pest is often serious Crops normally grow for impact, make a large amount of underproduction of crops, bring huge economic loss to grower.Therefore, to farming Thing insect pest has to effectively be prevented and treated, it is ensured that the economic interests of grain security and grower.
The insect of make inroads on crop has many kinds now, most importantly insecticide, lepidoptera pest, Hymenoptera insect, double Wing mesh, Hemipteran pest and coleopteran pest, common insect has Bemisia tabaci, diamondback moth, Bombycis mori, Pyrausta nubilalis (Hubern)., coccid, Gold Testudiniss Son, black peach aphid, trialeurodes vaporariorum etc., seriously cause a large amount of underproduction of crops or Quality Down, directly damage the economic interests of grower.
Paecilomyces fumosoroseus (Paecilomycesfumosoroeus) geographical distribution is extensive, and insect host is various, is important One of insect pathogenic fungus, be paecilomyces, be mainly characterized by conidiophore in ampuliform or subsphaeroidal, at mycelia end or short Verticillate on side shoot.The host range of paecilomyces fumosoroseus is wide, can parasitic various insects, including Homoptera, Lepidoptera, Diptera, Coleoptera, Hymenoptera etc., such as Bemisia tabaci, brown paddy plant hopper, coccid, aphid, Bombycis mori, cloud geometrid moth, housefly etc..Existing rose dark brown at present The single dose drug effect of Paecilomyces varioti pesticide is poor, it is impossible to which Field Pests are effectively prevented and treated.Now for paecilomyces fumosoroseus pesticide Exploitation and using many using with other insecticide mixtures by the way of carry out the preventing and treating of Field Pests.Therefore, exploitation has high preventive effect Paecilomyces fumosoroseus single dose pesticide be the difficult problem for facing at present.
Content of the invention
For problems of the prior art, the present invention provides a kind of paecilomyces fumosoroseus granule and its preparation side Method.
The specific technical scheme of the present invention is as follows:
The invention provides a kind of paecilomyces fumosoroseus granule, including the component of following parts by weight:
5~15 parts of medicine activity component,
10~25 parts of adjuvant;
50~90 parts of pharmaceutical carrier;
15~25 parts of water;
Wherein, described medicine activity component is that paecilomyces fumosoroseus, the paecilomyces fumosoroseus are compiled selected from biological deposits Number for CGMCC No.7993 strain.
Further, the spore content of described paecilomyces fumosoroseus granule be hundred million spore of 10-200/gram.
Further, miscellaneous bacteria rate≤0.1% of described paecilomyces fumosoroseus granule.
Further, the granularity of described paecilomyces fumosoroseus granule is 400 μm~2200 μm.
Further, expulsion rate≤3% of described paecilomyces fumosoroseus.
Further, the adjuvant includes glucose and/or corn starch.
Further, the pharmaceutical carrier includes in kieselguhr, Pulvis Talci, white carbon, sodium sulfate, potter's clay and Kaolin Plant or several.
Further, the preparation method of the paecilomyces fumosoroseus granule, it is characterised in that comprise the following steps:
1) pharmaceutical carrier, medicine activity component and adjuvant mixed, crush, obtain powder;
2) by the step 1) powder that obtains mixed with water, pelletize, obtains paecilomyces fumosoroseus granule.
Further, application of the described paecilomyces fumosoroseus granule in terms of preventing and treating crop pests.
The paecilomyces fumosoroseus granule of the present invention is efficient, low toxicity, and insecticidal effect is stable, and to people and animals and crop safety, Safe.Test result indicate that, the effect of paecilomyces fumosoroseus preventing and treating crop pests is more than 88%.
Biological deposits explanation
Paecilomyces fumosoroseus (Paecilomycesfumosa-roseus), are deposited in Chinese micro- life on the 13rd in August in 2013 Thing culture presevation administration committee common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese section Institute of microbiology of institute, biological deposits numbering is CGMCC No.7993.
Specific embodiment
The invention provides a kind of paecilomyces fumosoroseus granule, including the component of following parts by weight:Pharmaceutically active group Divide 5~15 parts, described medicine activity component is paecilomyces fumosoroseus;10~25 parts of adjuvant;50~90 parts of pharmaceutical carrier;Water 15~25 parts.
The paecilomyces fumosoroseus granule that the present invention is provided preferably includes 5~15 parts of paecilomyces fumosoroseus, and more preferably 8 ~12 parts, most preferably 10 parts.
In the present invention, the paecilomyces fumosoroseus are preferably selected from the bacterium that biological deposits numbering is CGMCC No.7993 Plant activation culture to obtain.
In the present invention, the preparation method of the paecilomyces fumosoroseus preferably includes following steps:
1) Paecilomyces Fumosoroseus kind is activated;
2) by the step 1) paecilomyces fumosoroseus after the activation that obtains are inoculated in the training of paecilomyces fumosoroseus level liquid Level liquid culture is carried out in foster base, obtain paecilomyces fumosoroseus primary seed solution;
3) by the step 2) primary seed solution that obtains is inoculated in paecilomyces fumosoroseus secondary liquid culture medium and carries out Secondary liquid culture, obtains paecilomyces fumosoroseus secondary seed solution;
The secondary liquid culture medium includes the component of following mass content:20~30g/L of analysis for soybean powder, white sugar 20~ 40g/L, 0.05~0.15g/L of sodium nitrate, 5~15g/L of Semen Maydis powder, 0.1~0.8g/L of dipotassium hydrogen phosphate, potassium chloride 0.20~ 0.30g/L, 0.01~0.08g/L of iron sulfate, 0.01~0.08g/L of magnesium sulfate, 0.01~0.08g/L of manganese sulfate, balance of water;
4) by the step 3) secondary seed solution that obtains is inoculated in paecilomyces fumosoroseus solid medium, carries out solid Culture, obtains paecilomyces fumosoroseus.
In the present invention, the paecilomyces fumosoroseus are preferably selected from the bacterium that biological deposits numbering is CGMCC No.7993 Plant activation culture to obtain.
In the present invention, the activation of Paecilomyces Fumosoroseus kind is that the Paecilomyces Fumosoroseus kind of preservation is inoculated in equipped with battalion In the eggplant type bottle of foster agar culture medium, 25~30 DEG C of 10~14h of culture carry out activation culture.
The paecilomyces fumosoroseus for activating are obtained, the paecilomyces fumosoroseus of the activation are inoculated into rose dark brown and intend green grass or young crops by the present invention Paecilomyces fumosoroseus level liquid culture is carried out in mould level liquid culture medium, obtains paecilomyces fumosoroseus primary seed solution.
In the present invention, the time of the level liquid culture is preferably 25~40h, more preferably 30~35h, most has choosing For 32h.The temperature of the level liquid culture is preferably 20~30 DEG C, more preferably 23~28 DEG C, most preferably 25 DEG C.Described The original ph of level liquid culture preferably 6.0~7.0, more preferably 6.4~6.8, most preferably 6.5.The level liquid The inoculum concentration of culture is preferably 0.5~1.5%, more preferably 0.8~1.2%, most preferably 1.0%.
In the present invention, the level liquid culture needs to carry out under agitation, and the frequency of stirring is preferably 1~2 time/h, More preferably 2 times/h;The time of stirring is preferably 10~20min, most preferably more preferably 12~18min, 15min every time. The present invention does not have special restriction to the equipment of the stirring, using mixing plant well known to those skilled in the art.
In the present invention, ventilated in the process of level liquid culture, be particularly preferred as:Enter in culture 24h, ventilation Measure as 10~20m3/ h, after culture 24h, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is 15~24m, ventilation is3/h;More preferably:Enter in culture 24h, ventilation is 12 ~18m3/ h, after culture 24h, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is 18~22m, ventilation is3/h;Most preferably:Enter in culture 24h, ventilation is 15m3/ h, training After foster 24h, ventilation is 20m3/h.In the present invention, the air being passed through is particularly preferred as clean, dry, no miscellaneous bacteria Pure air.The present invention is not particularly limited to the equipment for being passed through pure air, using well known to those skilled in the art Aeration equipments, such as air compressor machine.In the present invention, the level liquid culture is cultivated under illumination condition.The present invention The method for providing illumination condition is not particularly limited, the side of the offer illumination condition that is routinely selected using those skilled in the art Method.In the present invention, the illumination condition is particularly preferred as adopting incandescent lighting.
In the present invention, the paecilomyces fumosoroseus level liquid culture medium preferably includes the component of following mass content: 25~40g/L of dehydrated potato powder, 15~25g/L of white sugar, 0.5~1.5g/L of peptone, 0.05~0.15g/L of sodium nitrate, chlorination 0.20~0.30g/L of potassium, 0.01~0.08g/L of magnesium sulfate, 0.01~0.08g/L of iron sulfate, 0.01~0.08g/L of manganese sulfate, 0.1~0.8g/L of dipotassium hydrogen phosphate, balance of water;More preferably:30~35g/L of dehydrated potato powder, 18~22g/L of white sugar, egg White 0.8~1.2g/L of peptone, 0.08~0.12g/L of sodium nitrate, 0.22~0.27g/L of potassium chloride, 0.03~0.07g/L of magnesium sulfate, 0.03~0.07g/L of iron sulfate, 0.03~0.07g/L of manganese sulfate, 0.3~0.7g/L of dipotassium hydrogen phosphate, balance of water;Most preferably For:Dehydrated potato powder 33g/L, white sugar 20g/L, peptone 1.0g/L, sodium nitrate 0.1g/L, potassium chloride 0.25g/L, magnesium sulfate 0.05g/L, iron sulfate 0.05g/L, manganese sulfate 0.05g/L, dipotassium hydrogen phosphate 0.5g/L, balance of water.The present invention is to above-mentioned medicine The source of product is not particularly limited, and is conventionally used for preparing the medicine of culture medium using those skilled in the art.
After obtaining paecilomyces fumosoroseus primary seed solution, the one-level paecilomyces fumosoroseus primary seed solution is connect by the present invention Planting carries out paecilomyces fumosoroseus secondary liquid culture in paecilomyces fumosoroseus secondary liquid culture medium, obtains paecilomyces fumosoroseus Secondary seed solution.
In the present invention, the time of the paecilomyces fumosoroseus secondary liquid culture preferably 40~56h, more preferably 45 ~50h, most preferably 48h;The temperature of the secondary liquid culture is preferably 20~30 DEG C, more preferably 23~28 DEG C, optimum Elect 25 DEG C as;The original ph of the secondary liquid culture is preferably 6.0~7.0, more preferably 6.3~6.7, most preferably 6.5;The inoculum concentration of the secondary liquid culture is preferably 5~15%, more preferably 8~12%, most preferably 10%.
In the present invention, air being passed through in secondary liquid incubation, is particularly preferred as entering in culture 24h, ventilation For 130~170m3/ h, after culture 24h, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is 200~260m, ventilation is3/h;More preferably enter in culture 24h, ventilation is 140~160m3/ h, after culture 24h, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is 210~250m, ventilation is3/h;Most preferably enter in culture 24h, ventilation is 150m3/ h, after culture 24h, ventilation is 230m3/h.In the present invention, the air being passed through is particularly preferred as clean, dry Dry, no miscellaneous bacteria pure air.The present invention is not particularly limited to the equipment for being passed through pure air, using art technology Aeration equipments known to personnel, such as air compressor machine.
In the present invention, the secondary liquid culture needs to carry out under agitation, and the frequency of stirring is preferably 1~2 time/h, More preferably 2 times/h;The time of stirring is preferably 10~20min, most preferably more preferably 12~18min, 15min every time. The present invention does not have special restriction to the equipment of the stirring, using mixing plant well known to those skilled in the art.
In the present invention, the secondary liquid culture is cultivated under illumination condition.The present invention is to providing illumination condition Method be not particularly limited, the method for the offer illumination condition that is routinely selected using those skilled in the art.At this In bright, the illumination condition is particularly preferred as adopting incandescent lighting.
In the present invention, the secondary liquid culture medium preferably includes the component of following mass content:Analysis for soybean powder 20~ 30g/L, 20~40g/L of white sugar, 0.05~0.15g/L of sodium nitrate, 5~15g/L of Semen Maydis powder, 0.1~0.8g/ of dipotassium hydrogen phosphate L, 0.20~0.30g/L of potassium chloride, 0.01~0.08g/L of iron sulfate, 0.01~0.08g/L of magnesium sulfate, manganese sulfate 0.01~ 0.08g/L, balance of water;More preferably:23~28g/L of analysis for soybean powder, 25~35g/L of white sugar, 0.08~0.12g/ of sodium nitrate L, 8~12g/L of Semen Maydis powder, 0.3~0.7g/L of dipotassium hydrogen phosphate, 0.22~0.28g/L of potassium chloride, 0.03~0.07g/ of iron sulfate L, 0.03~0.07g/L of magnesium sulfate, 0.03~0.07g/L of manganese sulfate, balance of water;Most preferably:Analysis for soybean powder 25g/L, white sand Sugared 30g/L, sodium nitrate 0.1g/L, Semen Maydis powder 10g/L, dipotassium hydrogen phosphate 0.5g/L, potassium chloride 0.25g/L, iron sulfate 0.05g/ L, magnesium sulfate 0.05g/L, manganese sulfate 0.05g/L, balance of water.The present invention is not particularly limited to the source of above-mentioned medicine, is adopted It is conventionally used for preparing the medicine of culture medium with those skilled in the art.
In the present invention, the paecilomyces fumosoroseus secondary liquid culture medium preferably through 120~130 DEG C sterilizing 20~ Use after 45min.In the present invention, more preferably 121 DEG C of the sterilising temp;The sterilization time is more preferably 30min.This Invention is not particularly limited to the equipment of the sterilizing, using sterilizing installation well known to those skilled in the art, such as seed Tank.
After obtaining paecilomyces fumosoroseus secondary seed solution, the paecilomyces fumosoroseus secondary seed solution for obtaining is inoculated with by the present invention In paecilomyces fumosoroseus solid medium, solid culture is carried out, obtain paecilomyces fumosoroseus spore.
In the present invention, the inoculum concentration of the paecilomyces fumosoroseus solid culture is preferably 10~20%, more preferably 13 ~18%, most preferably 15%.In the present invention, the initial water content of the paecilomyces fumosoroseus solid medium is preferably 30 ~70%, more preferably 40~60%, most preferably 55%.In the present invention, the culture medium thickness of the solid culture are preferred For 1~6cm, most preferably more preferably 2~4cm, 3cm.
In the present invention, the control of the process temperature of the paecilomyces fumosoroseus solid culture and humidity, the temperature and The control program of humidity is particularly preferred as:It is 80~99% in the inner control loop border humidity for entering 1~3d of solid culture, environment temperature Spend for 24~30 DEG C, bacterium material temperature degree is controlled to 24~30 DEG C, enter 4~5d of solid culture inner control loop border humidity be 40~ 50%, ambient temperature is that 25~30 DEG C, within 6~7d, ambient temperature is 28~38 DEG C;More preferably:Entering solid culture 1 The inner control loop border humidity of~3d is 95%, and ambient temperature is that 28 DEG C, bacterium material temperature degree is controlled to 28 DEG C, is entering solid culture 4 The inner control loop border humidity of~5d is 45%, and it is temperature under natural conditions that ambient temperature is 28 DEG C, bacterium material temperature degree, within 6~7d Ambient temperature is 35 DEG C, and ambient humidity and bacterium material temperature degree are the humidity under natural conditions and temperature.
In the present invention, the paecilomyces fumosoroseus solid culture is preferably turned in 28~32h radiating, and more preferably 29 ~31h, most preferably 30h.
In the present invention, nutritional solution is preferably sprayed onto bacterium material in 45~55h by the paecilomyces fumosoroseus solid culture On, more preferably 48~52h, most preferably 50h.The fountain height of the nutritional solution is preferably the 5~10% of bacterium material quality, more excellent Elect 6~9%, most preferably 8% as.The present invention is not particularly limited to the equipment for spraying nutritional solution, using people in the art Spray appliance known to member.In the present invention, the nutritional solution preferably includes the component of following mass content:5% Fructus Vitis viniferae Sugar, 0.001% copper sulfate.The present invention is not particularly limited to the source of above-mentioned medicine, is routinely made using those skilled in the art Medicine.
In the present invention, the paecilomyces fumosoroseus solid medium preferably includes the component of following mass content:Testa Tritici 70~90%, Semen Maydis powder 5~15%, rice husk 5~15%, ammonium sulfate 0.5~1.5%;More preferably:Testa Tritici 75~85%, beautiful Rice flour 8~12%, rice husk 8~12%, ammonium sulfate 0.8~1.2%;Most preferably:Testa Tritici 79%, Semen Maydis powder 10%, rice husk 10%, ammonium sulfate 1.0%.The present invention does not have special restriction to the source of above-mentioned raw materials, using those skilled in the art's routine The raw material of configuration culture medium.
After the solid culture, preferred pair of the present invention obtains being collected for spore powder, obtains paecilomyces fumosoroseus spore. In embodiments of the present invention, the instrument that collects specifically using microorganism solid fermentation fungal spore separation equipment of the spore powder Carry out.The instrument of the microorganism solid fermentation fungal spore separation equipment is purchased from Jiangxi Tianren Ecology Co., Ltd..
In the present invention, effective spore amount of the paecilomyces fumosoroseus that the solid fermentation is obtained be 30,000,000,000 spores/gram with On.
The paecilomyces fumosoroseus granule that the present invention is provided preferably includes 10~25 parts of adjuvant, and more preferably 12~20 Part, most preferably 15 parts.The present invention does not have special restriction to the species of described adjuvant with source, using art technology The commercial goods of the adjuvant of the granular pesticide known to personnel.In specific preferably glucose and corn starch one Plant or two kinds.In the present invention, the adjuvant specifically preferable 7~15 parts of glucoses and 3~10 parts of corn starchs, more excellent Elect 8~12 parts of glucoses and 4~8 parts of corn starchs, most preferably 10 parts glucoses and 5 parts of corn starchs as.In the present invention, Described adjuvant makes medicine character stable, improves drug effect.
The paecilomyces fumosoroseus granule that the present invention is provided preferably includes 50~90 parts of pharmaceutical carriers, and more preferably 65~80 Part, most preferably 75 parts.In the present invention, the pharmaceutical carrier is preferably kieselguhr, Pulvis Talci, white carbon, sodium sulfate, potter's clay With one or more in Kaolin.The present invention does not have special restriction to the source of described pharmaceutical carrier, using commercially available business Product.In the present invention, the pharmaceutical carrier is specifically preferably for arbitrarily several in above-mentioned substance, can arbitrary proportion mix Close.In the present invention, the pharmaceutical carrier is specifically more preferably Pulvis Talci and Kaolin in above-mentioned substance, specifically can be excellent Elect 5~15 parts of Pulvis Talci and 45~75 parts of Kaolin, more preferably 8~12 parts Pulvis Talci and 60~68 parts of Kaolin as, optimum Elect 10 parts of Pulvis Talci and 65 parts of Kaolin as.Pharmaceutical carrier of the present invention has good medicine stability, can also improve The drug effect of medicine.
In the present invention, the paecilomyces fumosoroseus granule preferably includes 15~25 parts of water, more preferably 19~23 parts, Most preferably 22 parts.
The invention provides the preparation method of paecilomyces fumosoroseus granule described in technique scheme, including following step Suddenly:
1) pharmaceutical carrier, medicine activity component and adjuvant mixed, crush, obtain powder;
2) by the step 1) powder that obtains mixed with water, pelletize, obtains paecilomyces fumosoroseus granule.
In the present invention, the mixing of the pharmaceutical carrier, medicine activity component and adjuvant is preferably pharmaceutical carrier, medicine Thing active component and adjuvant are divided into 3~5 parts respectively, more preferably 4 parts, and the 1st part of pharmaceutical carrier and the 1st part of medicine are lived Property component mixing after, then mix with the 1st part of adjuvant, then mix with the 2nd part of pharmaceutical carrier again, then with the 2nd part of pharmaceutically active Component mixes, then mix with the 2nd part of adjuvant, by that analogy, until having added all of raw material, it is further continued for preferably mixing 40~ 80min, more preferably 50~70min, most preferably 60min.The present invention does not have special restriction to the equipment of the mixing, adopts With mixing apparatus well known to those skilled in the art, such as mixing filling.
In the present invention, 10~25 μm are preferably to the granularity of the powder, more preferably 12~20 μm, most preferably 15 μm.The present invention does not have special restriction to the condition of the crushing, using pulverization conditions well known to those skilled in the art. In the present invention, described crushing specific is preferably air-flowing type crushing, and the sealing pressure that air-flowing type is crushed is preferably 0.05 ~0.20MPa, more preferably 0.1~0.15MPa, most preferably 0.13MPa;The discharging sealing gland pressure that air-flowing type is crushed is preferably 0.1~0.2MPa, more preferably 0.15MPa;The pulsating pressure that air-flowing type is crushed preferably 0.3~0.7MPa, more preferably 0.4 ~0.6MPa, most preferably 0.5MPa;The feed pressure of air-flowing type preferably 0.7~0.9MPa, more preferably 0.75~ 0.85MPa, most preferably 0.8MPa.The present invention does not have special restriction to the equipment of the crushing, using people in the art Disintegrating apparatus known to member.
After obtaining powder, the powder and water mixing granulation are obtained paecilomyces fumosoroseus granule by the present invention.In the present invention In, the temperature of the pelletize is preferably 50~70 DEG C, more preferably 55~65 DEG C, most preferably 60 DEG C.The rose dark brown is intended blue or green The granularity of mould granule is preferably 400 μm~2200 μm, more preferably 500 μm~2100 μm, most preferably 600 μm~2000 μm. The present invention does not have special restriction to the equipment of the pelletize, using Granulation Equipments well known to those skilled in the art.
In the present invention, the spore content of described paecilomyces fumosoroseus granule preferably 10~20,000,000,000 spores/gram, more Preferably 30~18,000,000,000 spores/gram, most preferably 15,000,000,000 spores/gram.
In the present invention, miscellaneous bacteria rate≤0.1% of described paecilomyces fumosoroseus granule;Described paecilomyces fumosoroseus Expulsion rate≤3%.
Present invention also offers described in paecilomyces fumosoroseus granule described in technique scheme or technique scheme Application of the paecilomyces fumosoroseus granule that method is prepared in terms of preventing and treating crop pests.Specifically, applicable agriculture Crop species include cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..Rose dark brown of the present invention Paecilomyces varioti granule can prevent and treat each class pest, mainly sucking pest, including Bemisia tabaci, aphid, wood louse etc..
The present invention is not particularly limited to the application method of described paecilomyces fumosoroseus granule, using art technology Application method known to personnel.
Below in conjunction with the embodiment in the present invention, the technical scheme in the embodiment of the present invention is carried out clearly and completely Description, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on this Embodiment in bright, the every other enforcement obtained under the premise of creative work is not made by those of ordinary skill in the art Example, belongs to the scope of protection of the invention.
Embodiment 1
The preparation of paecilomyces fumosoroseus primary seed solution:The Paecilomyces Fumosoroseus kind of preservation is inoculated in equipped with nutrition fine jade In the eggplant type bottle of fat culture medium, 28 DEG C of culture 12h carry out activation culture, complete actication of culture;Paecilomyces fumosoroseus after activation Strain is inoculated in paecilomyces fumosoroseus level liquid culture medium according to 1% inoculum concentration, and level liquid culture medium includes following The component of mass content:Dehydrated potato powder 33g/L, white sugar 20g/L, peptone 1.0g/L, sodium nitrate 0.1g/L, potassium chloride 0.25g/L, magnesium sulfate 0.05g/L, iron sulfate 0.05g/L, manganese sulfate 0.05g/L, dipotassium hydrogen phosphate 0.5g/L, balance of water. The temperature of paecilomyces fumosoroseus level liquid culture is 25 DEG C, and original ph is 6.5, enters in culture 24h according to 15m3/ h leads to Tolerance carries out aerobic culture, according to 20m after liquid culture 24h3The ventilation of/h carries out aerobic culture, after illumination cultivation 32h, stops Only cultivate, obtain paecilomyces fumosoroseus primary seed solution.
The preparation of paecilomyces fumosoroseus secondary seed solution:By the paecilomyces fumosoroseus primary seed solution for obtaining with 10% inoculation Amount is inoculated in paecilomyces fumosoroseus secondary liquid culture medium, and secondary liquid culture medium includes the component of following mass content:Yellow Semen Glycines powder 25g/L, white sugar 30g/L, sodium nitrate 0.1g/L, Semen Maydis powder 10g/L, dipotassium hydrogen phosphate 0.5g/L, potassium chloride 0.25g/ L, iron sulfate 0.05g/L, magnesium sulfate 0.05g/L, manganese sulfate 0.05g/L, balance of water.The temperature of secondary liquid culture is 25 DEG C, pH value is 6.5, enters in culture 24h, and ventilation is 150m3/ h, after culture 24h, ventilation is 230m3/ h, is stirred per hour Mix 2 times, each mixing time is 15min, after illumination cultivation 48h, stops culture, obtains paecilomyces fumosoroseus secondary seed solution.
The solid culture of paecilomyces fumosoroseus:The paecilomyces fumosoroseus secondary seed solution for obtaining is connect with 15% inoculum concentration Plant in paecilomyces fumosoroseus solid medium, solid medium includes the component of following mass content:Testa Tritici 79%, Semen Maydis powder 10%, rice husk 10%, ammonium sulfate 1.0%.The initial water content of paecilomyces fumosoroseus solid medium is 55%, solid culture the 1d ambient temperature is 25 DEG C, and bacterium material temperature degree is that 25 DEG C, ambient humidity is cultivated under 95%;Solid culture 2d ambient temperature For 28 DEG C, bacterium material temperature degree is that 28 DEG C, ambient humidity is cultivated under 95%, is turned over when 30h is cultivated;Solid culture 3d ambient temperature is 28 DEG C, and bacterium material temperature degree is that 28 DEG C, ambient humidity is cultivated under 80%, when 50h is cultivated by 5% Fructus Vitis viniferae Sugar, the nutritional solution of 0.001% copper sulfate are sprayed onto on bacterium material, and the fountain height of nutritional solution is the 5% of bacterium material quality;Solid culture 4d ambient temperature is 28 DEG C, and ambient humidity is cultivated under 50%, and bacterium material temperature degree is the temperature under natural environment;Solid culture 5d ambient temperature is 28 DEG C, and ambient humidity is cultivated under 40%, and bacterium material temperature degree is the temperature under natural environment;Solid is trained Support 6d ambient temperature and be 30 DEG C, ambient humidity and bacterium material temperature degree are the humidity under natural conditions and temperature;Solid culture 7d Ambient temperature is 35 DEG C, and ambient humidity and bacterium material temperature degree are the humidity under natural conditions and temperature, after solid culture 7d, stop training Support, the effective spore amount of the paecilomyces fumosoroseus for obtaining is in 5,000,000,000 spore/more than g.
Bacterium material after solid culture is collected paecilomyces fumosoroseus using microbe in solid state culture fungal spore separation equipment Spore, after testing, effective spore amount of paecilomyces fumosoroseus be 30,000,000,000 spores/gram more than.
Embodiment 2
The preparation of paecilomyces fumosoroseus granule:In terms of parts by weight, by 10 parts of Pulvis Talci, 65 parts of Kaolin, 10 parts of realities Paecilomyces fumosoroseus, 10 parts of glucoses and the 5 parts of corn starchs for applying the preparation of example 1 are divided into 4 heaps respectively, by the 1st heap Pulvis Talci After mixing with the 1st heap Kaolin, then mix with the 1st heap paecilomyces fumosoroseus, then mix with the 1st heap glucose, then beautiful with the 1st heap Rice starch mixes, then mixes with the 2nd heap Pulvis Talci, by that analogy, until all raw materials have all mixed, is further continued for mixing 60min, obtains compound;Compound is crushed through air-flowing type, obtains 15 μm of powder;Powder adds 22 parts of water again, in temperature Under the conditions of 60 DEG C, 1200 μm of granule is manufactured into, obtains paecilomyces fumosoroseus granule.
The quality control index of paecilomyces fumosoroseus granule prepared by 1 embodiment of table 2
Project Index
Spore content, (hundred million spores/gram) 150±15
Spore rate living, % >= 90
Virulence (LT50, d:20-25℃)≤ 9
Miscellaneous bacteria rate, %≤ 0.1
Loss on drying, %≤ 6
PH range 5~7
Particle size range (600 μm -- 2000 μm), % >= 90
Expulsion rate, %≤ 3
Spore germination rate after preserving 2 years at bin stability (25 ± 2) DEG C, % >= 75
Bulk density, g/ml 0.55~0.85
Heap density, g/ml 0.60~0.90
Embodiment 3
The preparation of paecilomyces fumosoroseus granule:In terms of parts by weight, prepared by 75 parts of Kaolin, 10 parts of embodiments 1 Paecilomyces fumosoroseus, 15 parts of glucoses are divided into 4 heaps respectively, and the 1st heap Kaolin is mixed with the 1st heap paecilomyces fumosoroseus Afterwards, then with the 1st heap glucose mix, then mix with the 2nd heap Kaolin, by that analogy, until all raw materials have all mixed, then Continue mixing 50min, obtain compound;Compound is crushed through air-flowing type, obtains 10 μm of powder;Powder adds 25 parts again Water, under the conditions of temperature 60 C, is manufactured into 1600 μm of granule, obtains paecilomyces fumosoroseus granule.
Embodiment 4
The preparation of paecilomyces fumosoroseus granule:In terms of parts by weight, by 65 parts of Kaolin, 10 parts of kieselguhr, 15 parts of realities Apply the paecilomyces fumosoroseus of the preparation of example 1,7 parts of glucoses and 3 parts of corn starchs and be divided into 4 heaps respectively, by the 1st heap Kaolin with After the mixing of first heap kieselguhr, then mix with the 1st heap paecilomyces fumosoroseus, then mix with the 1st heap glucose, then beautiful with the first heap Rice starch mixes, then mixes with the 2nd heap Kaolin, by that analogy, until all raw materials have all mixed, is further continued for mixing 40min, obtains compound;Compound is being crushed through air-flowing type, obtains 12 μm of powder;Powder adds 20 parts of water again, in temperature Under the conditions of 50 DEG C of degree, 1000 μm of granule is manufactured into, obtains paecilomyces fumosoroseus granule.
Embodiment 5
The test of pesticide effectiveness:This test is carried out in Hebei Zhao County, prevents and treats pear sucker, and theatre management is good, during pear sucker occurs Deng weighting.Experimental field is divided into four regions by this test, and region 1 is matched group a, spray clear water;Region 2 sprays enforcement The 1000 times of diluents of paecilomyces fumosoroseus for preparing in example 2;It is dilute that region 3 sprays 5% 1000 times of efficient fluorine Cypermethrin microemulsion Release liquid;Region 4 sprays 90% trichlorphon, 1000 times of diluents;The dosage in four regions is identical, 1 day after dispenser, 3 days, and 5 My god, respectively investigate borer population of once living within 7 days, calculate Revision insect recluced rate.Concrete outcome is as shown in table 2, can be drawn by the data of table 2, The Revision insect recluced rate highest in region 2, shows that the insecticidal effect of paecilomyces fumosoroseus granule prepared by the present invention is best, and which is 7 days Drug effect is above metrifonate and efficient fluorine Cypermethrin.
Parasite killing (pear sucker) effect of 2 paecilomyces fumosoroseus granule of table
Can draw from the test data of table 2, the paecilomyces fumosoroseus granule Revision insect recluced rate of 7 days prepared by the present invention 88.6% is may be up to, its insecticidal effect is better than efficient fluorine Cypermethrin and metrifonate, the lasting period is long.
Embodiment 6
The test of pesticide effectiveness:This test is carried out in Earthquake of Anyang station in Henan, prevents and treats wheat aphid.Area of wheat management is good, during aphid occurs Deng weighting.Experimental field is divided into four regions by this test, and region 1 is matched group a, spray clear water;Region 2 sprays enforcement The 1000 times of diluents of paecilomyces fumosoroseus for preparing in example 2;It is dilute that region 3 sprays 5% 1000 times of efficient fluorine Cypermethrin microemulsion Release liquid;Region 4 sprays 90% trichlorphon, 1000 times of diluents;The dosage in four regions is identical, 1 day after dispenser, 3 days, and 5 My god, respectively investigate borer population of once living within 7 days, calculate Revision insect recluced rate.Concrete outcome is as shown in table 2, can be drawn by the data of table 2, The Revision insect recluced rate highest in region 2, shows that the insecticidal effect of paecilomyces fumosoroseus granule prepared by the present invention is best, and which is 7 days Drug effect is above metrifonate and efficient fluorine Cypermethrin.
Parasite killing (aphid) effect of 3 paecilomyces fumosoroseus granule of table
Can draw from the test data of table 2, the paecilomyces fumosoroseus granule Revision insect recluced rate of 7 days prepared by the present invention 89.8% is may be up to, its insecticidal effect is better than efficient fluorine Cypermethrin and metrifonate, the lasting period is long.
It can be professional and technical personnel in the field's realization or use that above-mentioned embodiment is intended to illustrate the present invention, to above-mentioned Embodiment is modified for those skilled in the art and be will be apparent, therefore the present invention is included but is not limited to Above-mentioned embodiment, any meet the claims or description description, meet with principles disclosed herein and novelty, The method, technique of inventive features, product, each fall within protection scope of the present invention.

Claims (9)

1. a kind of paecilomyces fumosoroseus granule, it is characterised in that including the component of following parts by weight:
5~15 parts of medicine activity component,
10~25 parts of adjuvant;
50~90 parts of pharmaceutical carrier;
15~25 parts of water;
Wherein, described medicine activity component is that paecilomyces fumosoroseus, the paecilomyces fumosoroseus are selected from biological deposits numbering The strain of CGMCC No.7993.
2. a kind of paecilomyces fumosoroseus granule according to claim 1, it is characterised in that described paecilomyces fumosoroseus The spore content of granule be 10~20,000,000,000 spores/gram.
3. a kind of paecilomyces fumosoroseus granule according to claim 1 and 2, it is characterised in that described rose dark brown is intended Miscellaneous bacteria rate≤0.1% of penicillium sp granule.
4. a kind of paecilomyces fumosoroseus granule according to claim 1, it is characterised in that described paecilomyces fumosoroseus The granularity of granule is 400 μm~2200 μm.
5. a kind of paecilomyces fumosoroseus granule according to claim 1, it is characterised in that described paecilomyces fumosoroseus Expulsion rate≤3%.
6. paecilomyces fumosoroseus granule according to claim 1, it is characterised in that the adjuvant includes glucose And/or corn starch.
7. paecilomyces fumosoroseus granule according to claim 1, it is characterised in that the pharmaceutical carrier includes diatom One or more in soil, Pulvis Talci, white carbon, sodium sulfate, potter's clay and Kaolin.
8. the preparation method of the paecilomyces fumosoroseus granule described in claim 1~7 any one, it is characterised in that include Following steps:
1) pharmaceutical carrier, medicine activity component and adjuvant are mixed in proportion, crush, obtain powder;
2) by the step 1) powder that obtains mixed with water, pelletize, obtains paecilomyces fumosoroseus granule.
9. the paecilomyces fumosoroseus granule described in claim 1~7 any one is in the application of preventing and treating crop pests.
CN201610838326.0A 2016-09-22 2016-09-22 Paecilomyces fumosoroseus granules and preparing method thereof Pending CN106417282A (en)

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CN108770870A (en) * 2018-06-13 2018-11-09 马瑞燕 A kind of fumosorosea water dispersible granules and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN108522545A (en) * 2018-06-13 2018-09-14 山西农业大学 A kind of fumosorosea wettable powder and preparation method thereof
CN108770870A (en) * 2018-06-13 2018-11-09 马瑞燕 A kind of fumosorosea water dispersible granules and preparation method thereof
CN108522545B (en) * 2018-06-13 2021-04-06 山西农业大学 Isaria fumosorosea wettable powder and preparation method thereof
CN108770870B (en) * 2018-06-13 2021-06-18 山西农业大学 Isaria fumosorosea water dispersible granule and preparation method thereof

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Application publication date: 20170222