CN102613281B - Preparation method for south China pine needle extractives and application of south China pine needle extractive in freshness of eggs - Google Patents
Preparation method for south China pine needle extractives and application of south China pine needle extractive in freshness of eggs Download PDFInfo
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- CN102613281B CN102613281B CN 201210115176 CN201210115176A CN102613281B CN 102613281 B CN102613281 B CN 102613281B CN 201210115176 CN201210115176 CN 201210115176 CN 201210115176 A CN201210115176 A CN 201210115176A CN 102613281 B CN102613281 B CN 102613281B
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Abstract
The invention relates to a preparation method for south China pine needle extractives and application of south China pine needle extractives in freshness of eggs. The preparation method comprises the steps of collecting and treating pine needles, then freezing and thawing repeatedly, performing ethanol digestion and finally filtering and concentration. The preparation method uses fresh, tender and non-toxic pine needles to be cleaned, dried and smashed, repeatedly freezes and thaws for 2 to 3 times, uses 45% of ethanol solution to perform digestion, and decompresses and concentrates to obtain pine needle extractives. The pine needle extractives are applied to freshness of eggs to prolong quality guarantee period. The preparation method for south China pine needle extractives and application of south China pine needle extractives in freshness of eggs are low in production cost, high in product yield, safe, reliable, green, free of pollution, antibacterial and capable of retaining freshness to achieve the purpose of prolonging shelf time of eggs.
Description
Technical field
The invention belongs to plant extracts research and applied technical field, relate to the efficient freeze thawing extraction of a kind of usefulness and produce extract from pine needles, and utilize the characteristics such as extract is antibacterial, anti-oxidant and antiviral, carry out south China pine extract from pine needles preparation method and the application in Fresh preservation for fowl eggs thereof of Fresh preservation for fowl eggs.
Background technology
Research finds that extract from pine needles contains the various active composition, comprises volatility oils, polyatomic phenol, flavonoids, glucoside and terpene etc. functional mass, has anti-oxidant, expelling parasite, many biological actions such as antibacterial and antiviral.The research and development extract from pine needles is one of the development trend in following plant extracts field.Extract from pine needles relies on its unique and complex physiological function to become gradually the study hotspot of the subjects such as nutrition, physiology, immunology, its Related product is by a plurality of industry extensive uses, such as industries such as medicine, herding and daily use chemicals, yet there are no report and be used for Fresh preservation for fowl eggs.
Because extract from pine needles has abundant active component, people are for the extraction of its active component and application technology also pay attention to day by day.At present, the isolation technics about extract from pine needles mainly contains aqueous solution low temperature extraction method, steam distillation, extraction with aqueous solution method, organic solvent extraction method, supercritical CO
2Extraction and enzymatic isolation method etc., but have that recovery rate is low, the different problems such as solvent consumption is large, secondary pollution, process is loaded down with trivial details or investment of production is large.
The used freeze thawing extraction of the present invention has that safety non-pollution, technique are simple, cost is lower, and the characteristics such as be conducive to accomplish scale production.In addition, about the existing large quantity research of applying of extract from pine needles, a kind of wet-land pine tree water extract and the application in food fresh keeping thereof have been reported such as Chinese patent application 200810162531.5.This method utilizes the antibiotic and anti-oxidation characteristics of wet-land pine tree water extract to carry out food fresh-keeping, but has the technology and method tradition, and extract yield is low, and composition is laid particular stress on the defectives such as water-soluble substances, and without the Fresh preservation for fowl eggs example.
Summary of the invention
For overcoming above-mentioned technical disadvantages, the invention provides the efficient freeze thawing extraction of a kind of usefulness and produce extract from pine needles, and utilize the characteristics such as extract is antibacterial, anti-oxidant and antiviral, carry out south China pine extract from pine needles preparation method and the application in Fresh preservation for fowl eggs thereof of Fresh preservation for fowl eggs.
The present invention solves the technical method that its technical problem adopts: a kind of south China pine extract from pine needles preparation method, and its processing step is:
The first step is pine needle collection and processing: non-destructive gathers fresh pollution-free, the disease-free light green south China pine pine needle of middle-aged forest, and selected removal of impurities is clean with the clear water rinsing, drains rear pulverizing making beating;
Second step is multigelation: pine needle slurry is successively put under the storage requirement of-25 ℃ and 4 ℃, multigelation 2～3 times impels pine needle lysis, discharges its active ingredient;
The 3rd step was alcohol steep: the pine needle that thaws slurry is the pine needle slurry by volume: mass percent concentration is that 45% ethanolic solution is 1: 5, carries out 75 ℃ of water-bath backflows, lixiviate 12h, mistake leaching filtrate;
The 4th step was filtering and concentrating: on go on foot again lixiviate 6h of filter residue, cross leaching filtrate, incorporate again step filtrate into, be evaporated to medicinal extract or drying and crushing, obtain south China pine extract from pine needles, it is for subsequent use to seal rear 4 ℃ of refrigerations.
The application of a kind of south China pine extract from pine needles in Fresh preservation for fowl eggs, get the extract from pine needles pulvis that is made by said method in right amount, adding distil water is prepared, by weight being the extract from pine needles pulvis: distilled water is 20～80:1000 preparation liniment, after smearing egg or quail egg shell with above-mentioned liniment, carry out fresh-retaining preserving.
The invention has the beneficial effects as follows: application freeze thawing extraction technology of the present invention, wherein frozen-thaw process is conducive to pine needle lysis, the release that promotes active ingredient with separate; And with 45% ethanolic solution lixiviate, impel separating out that water-soluble and pure melt into divides, and increase the sufficient content of fresh-keeping activity in the extract, to compare the product yield higher with traditional formulation, and biological value is better; Solvent for use nonhazardous effect of the present invention, and extraction effect is comparatively desirable, can not cause the secondary pollution in the extract from pine needles leaching process, brings ectogenic harmful substance into to product; In addition, used alcohol solvent can repeatedly utilize, and reduces production costs; Technological process of the present invention is simple, and equipment is easy to get, and is workable, is conducive to accomplish scale production; The pine needle slag that produces during extract from pine needles is produced is rich in the nutritional labelings such as trace element and cellulose, and the safety non-toxic evil, can be used as fertilizer developed, and promotes the comprehensive utilization of resource; The gained extract from pine needles can add other function ingredients, such as polysaccharide, calcium propionate or littleization starch etc., to improve its anti-corrosive fresh-keeping effect, also can capsule or the form such as tablet be developed to the physiology that the human or animal uses and adjust.
The present invention adopts fresh and tender nonhazardous pine needle to pulverize after clean draining, multigelation 2～3 times, and 45% ethanolic solution lixiviate is evaporated to medicinal extract, obtains extract from pine needles, carries out Fresh preservation for fowl eggs with it again, extends the shelf life.Produce extract from pine needles and Fresh preservation for fowl eggs with this law, not only production cost is slightly low, and the product yield is higher, and safe and reliable, and green non-pollution, and energy anti-bacterial refreshing reach the purpose that prolongs the birds, beasts and eggs shelf life.
Description of drawings
Fig. 1 is technological process block-diagram of the present invention.
The specific embodiment
The present invention is further described below in conjunction with embodiment.
Referring to Fig. 1, a kind of south China pine extract from pine needles preparation method and the application in Fresh preservation for fowl eggs thereof, its processing step is:
The first step is pine needle collection and processing: non-destructive gathers fresh pollution-free, the disease-free light green south China pine pine needle of middle-aged forest, and selected removal of impurities is clean with the clear water rinsing, drains rear pulverizing making beating;
Second step is multigelation: pine needle slurry is successively put under the storage requirement of-25 ℃ and 4 ℃, multigelation 2～3 times impels pine needle lysis, discharges its active ingredient;
The 3rd step was alcohol steep: the pine needle that thaws slurry is the pine needle slurry by volume: mass percent concentration is that 45% ethanolic solution is 1: 5, carries out 75 ℃ of water-bath backflows, lixiviate 12h, mistake leaching filtrate;
The 4th step was filtering and concentrating: on go on foot again lixiviate 6h of filter residue, cross leaching filtrate, incorporate again step filtrate into, be evaporated to medicinal extract or drying and crushing, obtain south China pine extract from pine needles, it is for subsequent use to seal rear 4 ℃ of refrigerations;
The application of a kind of south China pine extract from pine needles in Fresh preservation for fowl eggs, get the extract from pine needles pulvis that is made by said method in right amount, adding distil water is prepared, by weight being the extract from pine needles pulvis: distilled water is 20～80:1000 preparation liniment, after smearing egg or quail egg shell with above-mentioned liniment, carry out fresh-retaining preserving.
Embodiment one
A kind of south China pine extract from pine needles preparation method and the application in Fresh preservation for fowl eggs thereof,, its processing step is:
The pine needle collection is chosen fresh pollution-free, the disease-free light green south China pine pine needle 500g of middle-aged forest with processing, and is in time clean with the clear water rinsing, drains rear with pulverizer slurrying;
The filtering and concentrating filter residue is lixiviate 6h again, crosses leaching filtrate merging filtrate, is evaporated to medicinal extract, obtains south China pine extract from pine needles 65g, and 4 ℃ of refrigerations are for subsequent use;
Embodiment two
A kind of south China pine extract from pine needles preparation method and the application in Fresh preservation for fowl eggs thereof,, its processing step is:
Thaw pine needle slurry of alcohol steep adds 2.5 liter 45% ethanolic solution, carries out 75 ℃ of water-bath backflows, lixiviate 10h, mistake leaching filtrate;
The filtering and concentrating filter residue is lixiviate 6h again, crosses leaching filtrate merging filtrate, is evaporated to medicinal extract, obtains south China pine extract from pine needles 68g, and 4 ℃ of refrigerations are for subsequent use;
Application freeze thawing extraction technology of the present invention, wherein frozen-thaw process is conducive to pine needle lysis, the release that promotes active ingredient with separate; And with 45% ethanolic solution lixiviate, impel separating out that water-soluble and pure melt into divides, and increase the sufficient content of fresh-keeping activity in the extract, to compare the product yield higher with traditional formulation, and biological value is better; Solvent for use nonhazardous effect of the present invention, and extraction effect is comparatively desirable, can not cause the secondary pollution in the extract from pine needles leaching process, brings ectogenic harmful substance into to product; In addition, used alcohol solvent can repeatedly utilize, and reduces production costs; Technological process of the present invention is simple, and equipment is easy to get, and is workable, is conducive to accomplish scale production; The pine needle slag that produces during extract from pine needles is produced is rich in the nutritional labelings such as trace element and cellulose, and the safety non-toxic evil, can be used as fertilizer developed, and promotes the comprehensive utilization of resource; The gained extract from pine needles can add other function ingredients, such as polysaccharide, calcium propionate or littleization starch etc., to improve its anti-corrosive fresh-keeping effect, also can capsule or the form such as tablet be developed to the physiology that the human or animal uses and adjust.
The present invention adopts fresh and tender nonhazardous pine needle to pulverize after clean draining, multigelation 2～3 times, and 45% ethanolic solution lixiviate is evaporated to medicinal extract, obtains extract from pine needles, carries out Fresh preservation for fowl eggs with it again, extends the shelf life.Produce extract from pine needles and Fresh preservation for fowl eggs with this law, not only production cost is slightly low, and the product yield is higher, and safe and reliable, and green non-pollution, and energy anti-bacterial refreshing reach the purpose that prolongs the birds, beasts and eggs shelf life.
Claims (2)
1. south China pine extract from pine needles preparation method, it is characterized in that processing step is: the first step is pine needle collection and processing: non-destructive gathers fresh pollution-free, the disease-free light green south China pine pine needle of middle-aged forest, selected removal of impurities, clean with the clear water rinsing, drain rear pulverizing making beating;
Second step is multigelation: pine needle slurry is successively put under the storage requirement of-25 ℃ and 4 ℃, multigelation 2～3 times impels pine needle lysis, discharges its active ingredient;
The 3rd step was alcohol steep: the pine needle that thaws slurry is the pine needle slurry by volume: mass percent concentration is that 45% ethanolic solution is 1: 5, carries out 75 ℃ of water-bath backflows, lixiviate 12h, mistake leaching filtrate;
The 4th step was filtering and concentrating: on go on foot again lixiviate 6h of filter residue, cross leaching filtrate, incorporate again step filtrate into, be evaporated to medicinal extract or drying and crushing, obtain medicinal extract or the pulvis of south China pine extract from pine needles, it is for subsequent use to seal rear 4 ℃ of refrigerations.
2. the application of south China pine extract from pine needles in Fresh preservation for fowl eggs that preparation method as claimed in claim 1 makes, it is characterized in that: get an amount of extract from pine needles pulvis, adding distil water is prepared, by weight being the extract from pine needles pulvis: distilled water is 20～80:1000 preparation liniment, after smearing egg or quail egg shell with above-mentioned liniment, carry out fresh-retaining preserving.
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| CN103099291A (en) * | 2011-11-15 | 2013-05-15 | 浙江海洋学院 | Preparation method of natural preservative regarding red bayberry leaves as raw material |
| CN103828911A (en) * | 2012-11-21 | 2014-06-04 | 金华职业技术学院 | Fresh-keeping method of bergamot fruits with pine needle extract |
| CN106359564A (en) * | 2016-09-06 | 2017-02-01 | 合肥元政农林生态科技有限公司 | Preservation method for Petasites |
| CN106359565A (en) * | 2016-09-07 | 2017-02-01 | 合肥元政农林生态科技有限公司 | Osmund preservation method |
| CN106857801B (en) * | 2017-01-13 | 2019-11-15 | 曲阜师范大学 | A method of improving lycopene and lutein content in carrot |
| CN107412276A (en) * | 2017-08-25 | 2017-12-01 | 云南汉木森生物科技有限责任公司 | With the method for freeze-thaw method high efficiency extraction active ingredient of Chinese herbs |
| CN108238826A (en) * | 2018-01-29 | 2018-07-03 | 攀枝花市绿态美生物科技有限公司 | A kind of complex microorganism activity natural nutrition soil and preparation method thereof |
| CN114642216A (en) * | 2021-03-17 | 2022-06-21 | 王润泽 | Sterilizing composition containing plant composite stock solution and preparation method thereof |
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| CN101411468B (en) * | 2008-12-01 | 2012-07-04 | 浙江师范大学 | Pine needle extract of slash pine and use thereof in food fresh keeping |
| CN101690705B (en) * | 2009-08-21 | 2011-07-27 | 珈侬生化科技(中国)有限公司 | Preparation method of alpine pine needle active extract and whitening anti-aging active cosmetic |
| CN101810648A (en) * | 2010-03-16 | 2010-08-25 | 大连市敬益生物医药技术研究院 | Pine needle product and ultrasonic preparation method thereof |
| CN101804132B (en) * | 2010-04-20 | 2012-05-23 | 吉林农业大学 | Sansevieria trifasciata saponin, preparation method and application thereof |
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