CN101258859A - Motherwort total alkali mollucacide and preparation technique and using method thereof - Google Patents
Motherwort total alkali mollucacide and preparation technique and using method thereof Download PDFInfo
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- CN101258859A CN101258859A CNA2008100473175A CN200810047317A CN101258859A CN 101258859 A CN101258859 A CN 101258859A CN A2008100473175 A CNA2008100473175 A CN A2008100473175A CN 200810047317 A CN200810047317 A CN 200810047317A CN 101258859 A CN101258859 A CN 101258859A
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- total alkali
- siberian motherwort
- molluscacide
- oncomelania
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract
The invention relates to a snail-killing agent of total alkaloid granules in leonurus for perishing Oncomelania and a preparation technique and application method thereof. Fresh whole plant of the leonurus before the stage of full bloom is ground with appropriate amount of water and squeezed into liquor, then centrifugally separated or filtered; and dregs are aqueous-extracted and are filtered for residue removal; supernatant and filtered fluid are decompressed and concentrated to the specific gravity of 1.10 to 1.15, and absorbed by 732 cation exchange resin, then eluted by HCL with the concentration of 5 percent, decolorized and eluted by ethanol with the concentration of 95 percent, decompressed and dried to obtain the dried product of the total alkaloid in leonurus. Aqueous solution with the concentration of 0.60g/L to 1.0g/L prepared by the total alkaloid has obvious snail-killing effect; when the aqueous solution with the concentration of 0.60g/L is used for treating the Oncomelania for 4 to 5 days, the poisoning effect can reach 100 percent. The agent can be added into baits such as sugar powder and flour and then made into granules (the ratio of the total alkaloid in leonurus: the sugar powder: the flour: acacia equals to 1:5:40:5); the granules are bestrewed for 100g per hectare, the mortality of the Oncomelania can reach 100 percent after 3 days. The snail-killing agent of the invention has simple preparation technique, relatively low cost, convenient for storing, transporting and no pollution to the environment and no toxicity to human beings, livestock and fishes.
Description
Technical field
The present invention relates to a kind of siberian motherwort total alkali molluscacide particle molluscacide and preparation technology's and methods for using them thereof that is used for killing oncomelania.
Background technology
Schistosomiasis is the disease of serious harm human body health.Oncomelania is an indispensable link in the schistosomiasis propagation, and the spiral shell that goes out can reduce the propagation of schistosomiasis effectively.At present, snail-killing method roughly has three kinds: the spiral shell that manually goes out, chemicals spiral shell and the biology spiral shell that goes out that goes out.The time-consuming expense height of the spiral shell that manually goes out, the chemicals spiral shell that goes out costs an arm and a leg, and the term of validity is of short duration, and is how toxic to people, animal, fish etc., easily causes environmental pollution.
For fear of the pollution of chemicals molluscacide to environment, people seek out the pure natural plants snail killing agent always.The plant snail killing agents such as the plain molluscacide of rhizome powder molluscacide, Jatropha curcas of dioscorea zingiberensis wright have now been found.With the powder of plant or with the mixture composition of the plant extract spiral shell that goes out, effect can not be carried out the standardization evaluation to above-mentioned plant snail killing agent, is difficult to be approved in the world mostly.
Siberian motherwort [Leonurus artemisia (Lour.) S.Y.Hu] is a kind of annual upright draft, and high 60-100cm, leaf are to life, and stem is square, wheel umbrella inflorescence, and labiate corolla, the style base is given birth to, flower aubergine, nutlet brown.This plant is gynaecology's key medicine, can invigorate blood circulation, the stasis of blood of dispelling, menstruation regulating, the water that disappears, and cures mainly irregular menstruation, the threatened abortion difficult labour, and retention of afterbirth, postpartum anemic fainting, abdominal pain due to blood stasis, metrorrhagia and metrostaxis, hematuria is rushed down blood, the swollen sore of carbuncle etc.Be born in hill wasteland, ridge, meadow and small stream limit etc. and locate, be distributed in temperate zone and subtropical zone.China widely cultivates in the schistosomiasis epidemic-stricken area.Resource is very abundant.Find that after deliberation siberian motherwort has very strong toxic action to oncomelania, confirm that siberian motherwort total alkali wherein is one of the active component of spiral shell that mainly goes out.
Summary of the invention
The objective of the invention is in order to seek new plant snail killing agent, develop a kind of with siberian motherwort total alkali plant chemical ingredient particle molluscacide and preparation technology's and methods for using them thereof of (containing leonurine, leonurine first and stachydrine).
The present invention is the siberian motherwort total alkali that extracts with 732 cationic exchange resin adsorption methods the fresh complete stool of a kind of siberian motherwort before full-bloom stage, the plant chemical ingredient molluscacide of (containing leonurine, leonurine first and stachydrine).
Its preparation technology's flow process comprises the steps:
1) pluck, clean: pluck the preceding fresh herb of siberian motherwort of full-bloom stage, water cleans up;
2) above-mentioned raw materials adds suitable quantity of water and blends, and again with the crusher squeezing, the juice of squeezing out is with centrifuge centrifugation or filtration;
3) dregs of a decoction extract with 5～6 times of water gagings, filter, and remove filter residue after extracting 3 times;
4) supernatant after centrifugal and filtered fluid are evaporated to than behind the 1.1-1.15 that weighs last 732 cationic exchange resin adsorption;
5) with a small amount of washing 732 cation exchange resin columns, the discarded liquid that passes through;
6) carry out wash-out with 5%HCL;
7) AL on the eluent
2O
3Resin column; With 95% ethanol (containing 0.5 acetic acid) wash-out;
8) eluent is reclaimed ethanol;
9) use the concentrating under reduced pressure machine in 60-80 ℃ of concentrating under reduced pressure, drying, or use the freeze-drying freeze-drying, also available spray dryer promptly gets the spiral shell active chemical siberian motherwort total alkali that goes out in the siberian motherwort plant in 100-110 ℃ of condition atomized drying of following moment.
Siberian motherwort total alkali particle molluscacide preparation technology flow process comprises the steps: 1), to add what stir in every 1kg siberian motherwort total alkali be Icing Sugar, the flour of preparation in 4.5: 40: 4.5, the mixed powder 49kg of gum Arabic fine powder in proportion, add 1～5% proper amount of clear water, stir once more and make it even;
2) be pressed into the oodle maker of changing a social system, then and be about 0.8-1cm, the oval cylindrical particle of the about 0.2-0.4cm in footpath;
3), with heating using microwave to 70-90 ℃, be incubated 10-15 minute, being drying to obtain content is the siberian motherwort total alkali molluscacide of 2% specification.
The siberian motherwort total alkali spiral shell pulvis that goes out is the colourless crystallization powder, bitter, and non-volatility can be water-soluble, meets bismuth potassium iodide and generate red-brown precipitation; Oncomelania is had very strong killing action, and concentration is the aqueous solution processing time of 0.60g/L-1.0g/L to reach 3～5 days, and its dead spiral shell rate can reach 93.5%～100%; Toxic action increases with the increase of treatment fluid concentration and the prolongation in processing time, and has obvious seasonal difference; Summer, toxic action was the strongest, and spring, autumn take second place, and toxic action is little during winter oncomelania dormancy; Its toxic action mechanism is that oncomelania is caused oncomelania head, neck, foot and hepar damnification, and liver function is reduced; Responsive to the reaction of oncomelania esterase: enzymic activity changes the Changing Pattern that is " strengthening-weaken-disappear ".Oncomelania is poisoned early stage, the expression of medicine irritation EST, and the detoxification ability of enhancing oncomelania liver, enzyme is lived and is raise; Oncomelania is poisoned the middle and later periods, because drug treating time or intensity surpass its physiology threshold, the synthetic of enzyme is suppressed, and enzyme is lived and descended, until complete obiteration.
The present invention multiplies the go out application of spiral shell of region at schistosomiasis epidemic-stricken area oncomelania.
1) soak method extremely: be applicable to the shallow water area that can control water level, multiply the place as oncomelanias such as irrigation canals and ditches, small reservoir, rice fields, siberian motherwort total alkali particle molluscacide spreading is soaked oncomelania extremely in water, concentration is controlled at the 0.60-1.0g/L scope.
2) broadcast sowing method: by per hectare 100kg amount spreading, mortality of snails can reach 100% after 3 days with siberian motherwort total alkali particle molluscacide.
In sum, utilize the siberian motherwort total alkali particle molluscacide spiral shell that goes out, have following advantage:
1, aboundresources: siberian motherwort is distributed in temperate zone and subtropical zone.China widely cultivates in the schistosomiasis epidemic-stricken area.Resource is very abundant; Seminal propagation, growth is fast, and the output height can scale be cultivated, and the motherwort total alkali content is higher, and dry product content can reach more than 0.529%, does not have the problem of product development raw material difficulty; Be China's traditional Chinese medicine, economic worth is big, also has to view and admire, afforest, conserve water and soil and other comprehensive benefits.
2, production equipment is simple and easy: siberian motherwort total alkali production technology is easy, and production equipment and operation all do not have specific (special) requirements, production process environmentally safe; Be fit to the commercialization running, be particularly suitable for the various places medium-sized and small enterprises and utilize plant resources production on the spot.
3, easy to use: this product is convenient to store, transportation, according to actual sight, can spread, and also can soak and kill, and is extremely easy to use.Pure Phytochemistry composition, sn ail control effect are easy to carry out the standardization evaluation.
4, compliance with environmental protection requirements: this product sn ail control effect is continual and steady, and environmentally safe is to people and animals and fish nonhazardous, compliance with environmental protection requirements.
5, with low cost: this product integrated cost is lower than the chemistry spiral shell preparation that goes out, and economic benefit, social benefit, ecological benefits are obvious.
Embodiment
Embodiment 1
Get the preceding fresh herb 150kg of siberian motherwort of full-bloom stage,, get siberian motherwort total alkali particle molluscacide 7.94kg by the technological process preparation.
Embodiment 2
Get the preceding fresh herb 1000kg of siberian motherwort of full-bloom stage,, get siberian motherwort total alkali particle molluscacide 52.9kg by the technological process preparation.
The Application Example sn ail control effect is referring to annex 1, to the influences of fish referring to annex 2.
Annex 1: the test report of snail killing test of the present invention (retrial)
Infusion method is adopted in experiment.Experiment is controlled under 23 ± 1 ℃ of conditions in temperature to be carried out.Getting 100 on oncomelania alive is one group, in per 20 nylon net bags of packing into, respectively each group (5 bags every group) oncomelania is immersed in the glass jar of the motherwort total aqueous alkali that fills the 1000g variable concentrations, and other establishes the niclosamidum aqueous solution and the contrast of no chlorine clear water.Behind processing 1,2,3,4,5d, the oncomelania in each processing is taken out 1 bag at random, with no chlorine water flushing for several times, put into clear water again and leave standstill 1d, work is survived and checked then: open the operculum scrambler and be the spiral shell of living, the method for unloading thorn is broken and dissected to the usefulness of remainder into pieces, and discriminating is anyway.Experiment repeats 3 times, gets its mean value as experimental result (see Table 1 with table 2).
Table 1 sn ail control effect of the present invention
The aqueous solution of the present invention with variable concentrations is handled oncomelania, and under the processing of different time, mortality of snails there are differences.Its rule is that mortality of snails is in rising trend with the increase of handling concentration.0.60g/L-1.0g/L above motherwort total aqueous alkali has 100% obvious poisoning oncomelania lethal effect, even under very low concentration (0.20g/L-0.4g/L) is handled, also can reach the effect of poisoning with poison more than 30%; Motherwort total aqueous alkali with the above concentration of 1.5g/L is handled oncomelania 2d, just can reach 100% killing effect.
Niclosamidum is the chemical molluscacide that generally uses.Sn ail control effect of the present invention is contrasted with it, and sn ail control effect with 0.0005g/L niclosamidum solution is suitable approximately for the aqueous solution of the present invention of 0.60g/L as can be known.Drug effect but of the present invention is slightly slow than niclosamidum.2-3d can reach 100% lethality with 0.0005g/L niclosamidum solution-treated oncomelania, and handling with the aqueous solution of the present invention of 0.60g/L needs 4-5d just can reach same effect.
Annex 2: toxicity test of fishes of the present invention
1, the acute toxicity testing of fish
Fry (crucian and carp) is put into the aqueous solution of the present invention of four concentration (0.40g/L, 0.60g/L, 0.80g/L, 1.0g/L), observe the reaction of fry every day, no abnormal phenomenon takes place, it is also very brisk to move about, the statistics fry does not have death after 72 hours, after observing 48 hours again, the still no abnormal and phenomena of mortality.
2, the long term toxicity test of fish
Two kinds of fries of buy (carp and crucian) are put into four concentration of the present invention (20ppm, 40ppm, 200ppm, 400ppm) and the processing that clear water contrasts, measure the body length of fish, the body weight of title fish after 30 days respectively, carry out statistical analysis.Gained data and statistical analysis see the following form:
Table 1 young crucian is raised the body weight (gram) after 30 days
The variance analysis of the young crucian body weight of table 2 control group and processed group
From above variance analysis as can be known, F＜F
0.05, F＜F
0.01, the body weight of control group and each concentration group feeding fish does not have significant difference, and the visible aqueous solution of the present invention does not make significant difference to the body weight gain of crucian.
Table 3 young crucian is raised the body long (cm) after 30 days
The variance analysis that table 4 control group and processed group young crucian body are long
Above variance analysis as can be known, F＜F
0.05, F＜F
0.01, the long no significant difference of the young crucian body of processed group and control group, the aqueous solution of the present invention that is to say four concentration does not make significant difference to the growth of young crucian yet.
Raising to the carp fry in the aqueous solution of the present invention of above-mentioned four concentration does not make significant difference yet.
Data and variance analysis by experiment, the result shows: raise crucian and carp after one month in the variable concentrations the present invention who oncomelania is had strong killing effect, growing of carp and young crucian all do not made significant difference.
Claims (3)
1, a kind of siberian motherwort total alkali molluscacide is characterized in that the Leonurus extract (containing leonurine, leonurine first and stachydrine) that extracts with 732 cationic exchange resin adsorption methods the fresh complete stool of siberian motherwort before full-bloom stage is the plant chemical ingredient molluscacide of raw material.
2, a kind of preparation technology of siberian motherwort total alkali molluscacide is characterized in that comprising the steps:
1) pluck, clean: the fresh complete stool of siberian motherwort before the full-bloom stage of gathering, water cleans up;
2) above-mentioned raw materials is added suitable quantity of water and blend, again with the crusher squeezing, the juice of squeezing out centrifuge centrifugation or filtration;
3) dregs of a decoction extract with 5～6 times of water gagings, filter, and remove filter residue after extracting 3 times;
4) supernatant after centrifugal and filtered fluid are evaporated to than behind the 1.10-1.15 that weighs, use 732 cationic exchange resin adsorption;
5) with a small amount of washing 732 cation exchange resin columns, the discarded liquid that passes through;
6) carry out wash-out with 5%HCL;
7) AL on the eluent
2O
3Resin column absorption is then with 95% ethanol elution that contains 0.5% acetic acid;
8) to reclaiming ethanol by liquid;
9) use the concentrating under reduced pressure machine in 60-80 ℃ of concentrating under reduced pressure, drying, or use the freeze-drying freeze-drying, also available spray dryer promptly gets the spiral shell active chemical siberian motherwort total alkali that goes out in the siberian motherwort plant in 100-110 ℃ of condition atomized drying of following moment.
3, the preparation technology of a kind of siberian motherwort total alkali particle molluscacide according to claim 1 and 2 is characterized in that comprising the steps:
1), to add what stir in every 1kg siberian motherwort total alkali be Icing Sugar, the flour of preparation in 4.5: 40: 4.5, the mixed powder 49kg of gum Arabic fine powder in proportion, adds 1～5% proper amount of clear water, stir once more and make it even;
2) be pressed into the oodle maker of changing a social system, then and be about 0.8-1cm, the oval cylindrical particle of the about 0.2-0.4cm in footpath;
3), with heating using microwave to 70-90 ℃, be incubated 10-15 minute, being drying to obtain content is the siberian motherwort total alkali molluscacide of 2% specification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100473175A CN101258859A (en) | 2008-04-15 | 2008-04-15 | Motherwort total alkali mollucacide and preparation technique and using method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100473175A CN101258859A (en) | 2008-04-15 | 2008-04-15 | Motherwort total alkali mollucacide and preparation technique and using method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766332A (en) * | 2014-01-26 | 2014-05-07 | 江苏艾津农化有限责任公司 | Novel environment-friendly oncomelania killing granule capable of being sprayed and preparation method thereof |
| CN104522067A (en) * | 2014-12-16 | 2015-04-22 | 长阳勤劳农夫农产品有限公司 | Virus immunized inducer and application thereof |
| CN108812716A (en) * | 2018-08-03 | 2018-11-16 | 普定县顺和水果苗木种植有限公司 | A kind of biology molluscicide and preparation method thereof |
-
2008
- 2008-04-15 CN CNA2008100473175A patent/CN101258859A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766332A (en) * | 2014-01-26 | 2014-05-07 | 江苏艾津农化有限责任公司 | Novel environment-friendly oncomelania killing granule capable of being sprayed and preparation method thereof |
| CN103766332B (en) * | 2014-01-26 | 2016-05-18 | 江苏艾津农化有限责任公司 | A kind of novel environment friendly that can be used for spraying kills oncomelania granule and preparation method thereof |
| CN104522067A (en) * | 2014-12-16 | 2015-04-22 | 长阳勤劳农夫农产品有限公司 | Virus immunized inducer and application thereof |
| CN104522067B (en) * | 2014-12-16 | 2017-09-22 | 湖北勤劳农夫生态农业股份有限公司 | A kind of virus immunity type derivant and its application |
| CN108812716A (en) * | 2018-08-03 | 2018-11-16 | 普定县顺和水果苗木种植有限公司 | A kind of biology molluscicide and preparation method thereof |
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Application publication date: 20080910 |