CN100383054C - Method for preparing enteromorpha extract for inhibiting red tide algae and its use - Google Patents
Method for preparing enteromorpha extract for inhibiting red tide algae and its use Download PDFInfo
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- CN100383054C CN100383054C CNB2006100445871A CN200610044587A CN100383054C CN 100383054 C CN100383054 C CN 100383054C CN B2006100445871 A CNB2006100445871 A CN B2006100445871A CN 200610044587 A CN200610044587 A CN 200610044587A CN 100383054 C CN100383054 C CN 100383054C
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- tide algae
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Abstract
The present invention relates to a method for preparing an entermorpha extracting solution capable of inhibiting red tide algae and an application thereof. The method for preparing the entermorpha extracting solution capable of inhibiting red tide algae comprises the following steps that: enteromorpha linza plants or the pulverized enteromorpha linza plants are leached with water, ethyl alcohol or propanone, ethyl ether and chloroform or methyl alcohol; the obtained extracting solution is concentrated with a rotary evaporation method; the concentrated extracting solution is put into a water body to be processed to inhibit the growth of red tide algae in the water body to be processed. The enteromorpha linza extracting solution is applied to inhibit the growth of red tide algae; in general, per kilogram enteromorpha linza is leached in 30 to 50 liters of sterilized seawater for 1 to 3 days, and then the obtained extracting solution is put into the water body to be processed; 10 to 100 liters of the extracting solution is put into per liter water body; the inhibition ratio is more than 98%. The present invention makes full use of enteromorpha linza in the enteromorpha linza plants, has the advantages of high efficiency, safety and easy operation control, and can effectively inhibit the growth of harmful red tide algae in oceans.
Description
Technical field
The invention belongs to water treatment field, relate in particular to a kind of preparation method and application that suppresses the enteromorpha extract of red tide algae.
Background technology
After Lu Yuan nutritive substance and ocean operation discharging and the pollutent that leaks enter ocean water body, causing the seawater eutrophication, is the major cause that ocean environment worsens.Eutrophication causes the explosive growth of red tide algae, can reduce the dissolved oxygen of seawater, brings massive losses for the aquatic products aquaculture.Some algae can also produce the algae toxin, and feed water lively thing and human beings'health are brought harm.Middle Skeletonemacostatum is the coastal common and main red tide algae in Eastern China, therefore usually with the representative of middle Skeletonemacostatum as the red tide algae.
Existing red tide inhibitor is mainly the organic herbicide of cupric ion preparation and synthetic.Utilize cupric ion (Cu now
2+) to kill the red tide algae be to use the most sophisticated a kind of red tide inhibition technology, but cupric ion in water body, can not degrade, can in the ecosystem, transmit by food chain, and copper ion concentration high more (the biological amplification) in the high more organism of trophic level.If the people has eaten the enrichment hydrocoles of cupric ion, meeting serious harm HUMAN HEALTH.The artificial weeding agent can be killed other plant in the Marine ecosystems when killing the red tide algae, make water ecosystem destroyed, and ecological functions disappear, and finally makes the ocean become " Dead Sea " that biomass sharply descends.Therefore, need that exploitation is efficient badly, the red tide inhibitor of safety, easy operation control.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method and application that suppresses the enteromorpha extract of red tide algae, to overcome the deficiencies in the prior art.
The present invention is by lot of experiments, and filtering out from multiple kelp has remarkable inhibiting edge pipe Enteromorpha to the red tide algae.And then have the extracting solution of algal control function to drop into it to suppress the growth of red tide algae in seawater.Therefore with the extracting solution of edge pipe Enteromorpha as the application that suppresses the red tide algal grown.
The preparation method who suppresses the enteromorpha extract of red tide algae is: with solvent is sterilization seawater lixiviate edge pipe Enteromorpha, and the extracting solution that obtains is concentrated, and the extracting solution after concentrating is dropped into staying water, to suppress the growth of red tide algae in the staying water.Above-mentioned extracting technology rises sterilization seawater lixiviate 1-3 days with per kilogram edge pipe Enteromorpha with 30-50, and the extracting solution that obtains is concentrated into the 2-10% of original volume, and the extracting solution after concentrating is applied in the staying water, and every liter of staying water drops into 10-100 milliliter extracting solution.
The also available organic solvent of above-mentioned solvent, as ethanol, acetone, ether, chloroform or methyl alcohol etc., to be per kilogram edge pipe Enteromorpha rose ethanol, acetone, ether, chloroform or methyl alcohol lixiviate 12-48 hour with 2-5 to leach extraction method, apply in the staying water after the extracting solution that obtains is concentrated into 10%-20%, every liter of staying water drops into 1-5 milliliter concentrated extracting solution.Therefore, the extracting solution of edge pipe Enteromorpha is as the application that suppresses the red tide algae.
The present invention makes full use of the edge pipe Enteromorpha in the edge pipe Enteromorpha plant, and economical and efficient, good, the easy operation control of ecological security effectively suppress the particularly growth of middle Skeletonemacostatum of ocean harmful algal algae.
Embodiment
The preparation method who suppresses the enteromorpha extract of red tide algae is: with sterilization seawater lixiviate edge pipe Enteromorpha, adopt the rotary evaporation method to concentrate the extracting solution that obtains, the extracting solution after concentrating is dropped into staying water, to suppress the growth of red tide algae in the staying water.A kind of scheme is: per kilogram edge pipe Enteromorpha rises sterilization seawater lixiviate 1-3 days with 30-50, and the extracting solution that obtains is concentrated into the 2-10% of original volume, and the extracting solution after will concentrating applies in the staying water, and every liter of staying water drops into 10-100 milliliter extracting solution.Another kind of scheme is: per kilogram edge pipe Enteromorpha rose ethanol, acetone, ether, chloroform or methyl alcohol lixiviate 12-48 hour with 2-5, apply in the staying water after the extracting solution that obtains being concentrated into the 10%-20% of original volume, every liter of staying water drops into 1-5 milliliter concentrated extracting solution.Said extracted liquid can adopt rotary evaporation method etc. to concentrate.
Below by embodiment the specific embodiment of the present invention is described.
Embodiment 1
0.1 kilogram edge pipe Enteromorpha is with 3 liters of 2 days after-filtration of sea water immersion of sterilizing, filtrate is concentrated into 100 milliliters.Get concentrated solution 1,2,3,4,5 milliliters add respectively in 200 milliliters in the Skeletonemacostatum nutrient solution, and after 5 days, inhibiting rate is respectively 42%, 78%, and 94%, 97%, 99%.
Embodiment 2
1 kilogram of edge pipe Enteromorpha after 12 hours, 5 milliliter applies 200 milliliter in Skeletonemacostatum nutrient solution with the extracting solution that obtains with 2 liters of extraction using alcohols, and algae density only is 3% of control group after 5 days, and promptly inhibiting rate is 97%.
1 kilogram of edge pipe Enteromorpha after 48 hours, 5 milliliter applies 200 milliliter in Skeletonemacostatum nutrient solution with the extracting solution that obtains with 2 liters of extraction using alcohols, and algae density only is 2.5% of control group after 5 days, and promptly inhibiting rate is 97.5%.
Embodiment 3
1 kilogram of edge pipe Enteromorpha after 12 hours, 5 milliliter applies 200 milliliter in Skeletonemacostatum nutrient solution with the extracting solution that obtains with 2 liters of acetone extractions, and algae density only is 5% of control group after 5 days, and promptly inhibiting rate is 95%.
1 kilogram of edge pipe Enteromorpha after 24 hours, 5 milliliter applies 200 milliliter in Skeletonemacostatum nutrient solution with the extracting solution that obtains with 2 liters of acetone extractions, and algae density only is 2% of control group after 5 days, and promptly inhibiting rate is 98%.
1 kilogram of edge pipe Enteromorpha after 48 hours, 5 milliliter applies 200 milliliter in Skeletonemacostatum nutrient solution with the extracting solution that obtains with 2 liters of acetone extractions, and algae density only is 1.5% of control group after 5 days, and promptly inhibiting rate is 98.5%.
1 kilogram of edge pipe Enteromorpha after 48 hours, 5 milliliter applies 200 milliliter in Skeletonemacostatum nutrient solution with the extracting solution that obtains with 5 liters of acetone extractions, and algae density only is 7% of control group after 5 days, and promptly inhibiting rate is 93%.
Claims (6)
1. a preparation method who suppresses the enteromorpha extract of red tide algae at first with sterilization seawater lixiviate edge pipe Enteromorpha, is concentrated into the extracting solution that obtains the 2%-10% of original volume then.
2. the preparation method of the enteromorpha extract of inhibition red tide algae as claimed in claim 1 is characterized in that above-mentioned extracting technology is to rise sterilization seawater lixiviate 1-3 days with per kilogram edge pipe Enteromorpha with 30-50.
3. a preparation method who suppresses the enteromorpha extract of red tide algae at first uses solvent lixiviate edge pipe Enteromorpha, then the extracting solution that obtains is concentrated into the 10%-20% of original volume.
4. the preparation method of the enteromorpha extract of the described inhibition red tide of claim 3 algae is characterized in that above-mentioned solvent is ethanol, acetone or methyl alcohol.
5. the preparation method of the enteromorpha extract of the described inhibition red tide of claim 3 algae, it is characterized in that above-mentioned per kilogram edge pipe Enteromorpha rose ethanol, acetone or methyl alcohol lixiviate 12-48 hour with 2-5, apply in the staying water after the extracting solution that obtains is concentrated into 10%-20%, every liter of staying water drops into 1-5 milliliter concentrated extracting solution.
6. suppress the application of the enteromorpha extract of red tide algae, the extracting solution that it is characterized in that edge pipe Enteromorpha is as the application that suppresses the red tide algae.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100445871A CN100383054C (en) | 2006-05-26 | 2006-05-26 | Method for preparing enteromorpha extract for inhibiting red tide algae and its use |
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| CNB2006100445871A CN100383054C (en) | 2006-05-26 | 2006-05-26 | Method for preparing enteromorpha extract for inhibiting red tide algae and its use |
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| CN1850630A CN1850630A (en) | 2006-10-25 |
| CN100383054C true CN100383054C (en) | 2008-04-23 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101485343B (en) * | 2009-03-02 | 2013-06-05 | 中国海洋大学生物工程开发有限公司 | Extraction technology of active ingredients of enteromorpha and preparation method of enteromorpha seaweed fertilizer |
| CN103636679B (en) * | 2013-12-06 | 2015-01-28 | 淮海工学院 | Method for preparing phenolic acid and alkaloid from enteromorpha through separation and purification and application thereof |
| CN103725033B (en) * | 2013-12-24 | 2015-05-27 | 上海海洋大学 | Method for extracting Enteromorpha pigment |
| CN105879427A (en) * | 2016-05-09 | 2016-08-24 | 青岛海大生物集团有限公司 | Preparation method of enteromorpha biological anti-algae agent |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1354141A (en) * | 2000-11-17 | 2002-06-19 | 中国科学院海洋研究所 | Application of quaternaries compound for controlling seawater algae flower and fresh water algae flower |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1354141A (en) * | 2000-11-17 | 2002-06-19 | 中国科学院海洋研究所 | Application of quaternaries compound for controlling seawater algae flower and fresh water algae flower |
Non-Patent Citations (1)
| Title |
|---|
| 缘管浒苔对赤潮异藻的克生作用. 许研.生态学报，第25卷第10期. 2005 * |
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