CN101642134A - Preparation method of enteromorpha extract capable of inhibiting prococentrum marinum and phaeodactylum tricornutum bohlin - Google Patents
Preparation method of enteromorpha extract capable of inhibiting prococentrum marinum and phaeodactylum tricornutum bohlin Download PDFInfo
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- CN101642134A CN101642134A CN200910194502A CN200910194502A CN101642134A CN 101642134 A CN101642134 A CN 101642134A CN 200910194502 A CN200910194502 A CN 200910194502A CN 200910194502 A CN200910194502 A CN 200910194502A CN 101642134 A CN101642134 A CN 101642134A
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Abstract
The invention discloses a preparation method of enteromorpha extract capable of inhibiting prococentrum marinum and phaeodactylum tricornutum bohlin and can inhibit the red tide caused by prococentrummarinum and phaeodactylum tricornutum bohlin efficiently with low cost. The preparation method comprises the following steps: adopting fresh enteromorpha as raw material, adding distilled water to grind or pound to pieces, adding boiled seawater, centrifugating, centrifugating twice the obtained supernatant and finally obtaining the extract. The enteromorpha extract is added in rapidly increasingprococentrum marinum and phaeodactylum tricornutum bohlin and the maximum inhibition rate can reach 48-84% when the concentration of the extract is 0.6-1.0 g/L. the method has simple operation, low cost, environmental friend, high efficiency and high popularization and use value.
Description
Technical field:
The present invention relates to a kind of plant extract liquid and preparation method thereof, relate in particular to a kind of preparation method who suppresses the enteromorpha extract of ocean Prorocentrum and Phaeodactylum tricornutum.
Background technology:
Along with developing rapidly of explosive growth of population, industrial and agricultural production and improving constantly of living standards of the people, land-sourced pollutant increases the weight of day by day to the pollution of marine environment, cause the seawater eutrophication, cause coastal red tide to take place frequently thus all over the world, scale constantly enlarges, not only cause heavy economic losses, and endangering existent environment of people to fish production.
In order to effectively prevent and control red tide,, utilize biological inhibitory action between the plant self to administer the red tide shortcut of can yet be regarded as except carrying out high-frequency, high density monitoring.In the Freshwater ecosystems, higher plant and algae, the also existing more report of the competition effect between algae and algae.But between marine algae, seldom about the report of competition effect.
Phaeodactylum tricornutum (Phaeodactylum tricornutum) is one of red tide algae that has harm.Ocean Prorocentrum (Prorocentrum micans Ehrenb) is the common kind in inshore of Northern waters, the South Sea, also is the main kind that forms red tide.Two kinds of red tides all are difficult for administering.Enteromorpha (Enteromorpha) is large-scale green alga, and not only edible and medicinal has huge economic; And having characteristics such as growth is fast, the absorption nutriment is many, is that comparatively desirable marine ecology is repaired the marine alga kind.If can utilize large-scale green alga Enteromorpha as algicide, then can realize the red tide that low-cost little algae wawter bloom, especially Phaeodactylum tricornutum of control and ocean Prorocentrum cause, repair marine site ecology as early as possible.
Summary of the invention:
The objective of the invention is to provide a kind of simple method,, realize that low-cost, high efficiency suppresses the red tide that is caused by Phaeodactylum tricornutum and ocean Prorocentrum by utilizing large-scale green alga Enteromorpha Enteromorpha as algicide.
The object of the present invention is achieved like this: a kind of preparation method of enteromorpha extract that suppresses ocean Prorocentrum and Phaeodactylum tricornutum is provided, and is raw material with fresh Enteromorpha, and adding distil water grinds or smashs to pieces; Add seawater again through boiling, centrifugal; Gained supernatant repeatedly centrifugal 2-3 time again is an extract with final gained liquid.
Described centrifugal condition is: 3000 rev/mins, and 20 minutes.
Described enteromorpha extract optium concentration is 0.6-1.0g/L.
Described Enteromorpha is the bar Enteromorpha.
The present invention has the following advantages:
1, adopts grinding or the broken frond of the method for smashing to pieces, need not large-scale instrument and equipment, be fit to operation on the spot, simple and fast;
2, algae liquid extraction step is simple, extracting cycle short (1-2 hour), and treating capacity is arbitrarily control as required;
3, abundant raw material, low price, Enteromorpha be as large-scale economic algae, the coastal a large amount of breed of China;
4, Enteromorpha itself is born in the water, and the algae liquid that extracts in this method does not have secondary pollution to water body;
5, this improvement method is required artificial few, and labor cost is low;
6, to little algae inhibiting rate height, maximum can reach 84%.
Description of drawings
Fig. 1: different bar Enteromorpha extract concentration are to the influence of ocean Prorocentrum growth;
Fig. 2: the water-soluble extract of the bar Enteromorpha of variable concentrations is handled the upgrowth situation of Phaeodactylum tricornutum down.
Embodiment:
Below will be described in further detail, and provide and experimental results show that its inhibitory action a kind of preparation method of enteromorpha extract that suppresses ocean Prorocentrum and Phaeodactylum tricornutum of the present invention.
Embodiment 1
Experiment material is ocean Prorocentrum (Prorocentrum micans Ehrenb.), and it is the common kind in inshore of Northern waters, the South Sea, is the main kind that forms red tide, and concrete steps are as follows:
Get fresh Enteromorpha (Enteromorpha clathrata) 10g, adding distil water grinds pulping, with centrifugal 3 times of the seawater that has filtered scalding (centrifugal condition: 3000 rev/mins, 20 minutes), supernatant.Supernatant is pressed 0.6,0.7,0.8,0.9,1.0 (g/L of unit), 5 concentration gradients, and negative control, joining inoculum concentration is in the ocean Prorocentrum solution of 3000/mL, cultivated altogether 10 days, sampling numeration in per 2 days, and write down its variation.
The result shows, under the identical ocean Prorocentrum inoculum concentration (3000/mL) growth of the ocean Prorocentrum of adding Enteromorpha extract significantly descends, and Enteromorpha extract concentration is big more, the ocean Prorocentrum growing state is poor more, when Enteromorpha extract concentration is 0.9g/L, at the 10th day ocean Prorocentrum is suppressed to reach maximum, inhibiting rate is 48.2%.Different bar Enteromorpha extract concentration are seen Fig. 1 to the influence of ocean Prorocentrum growth.
Experiment material is Phaeodactylum tricornutum (Phaeodactylum tricornutum), and concrete steps are as follows:
Get fresh Enteromorpha 10g, adding distil water grinds pulping, with centrifugal 4 times of the seawater that has filtered scalding (centrifugal condition: 3000 rev/mins, 20 minutes), supernatant.With this liquid is mother liquor, uses through the seawater of scalding and makes different dilution ratios, and the concentration gradient that makes the water-soluble extract of Enteromorpha is 0,0.3,0.4,0.5,0.6,0.7 (g/L of unit), and inoculation is in the Phaeodactylum tricornutum (inoculum concentration 1 * 10 of exponential phase respectively
4Cells/mL).
The result shows that in experimentation, the water-soluble extract of the Enteromorpha of 0.3-0.7g/L all has remarkable inhibitory action to the growth of Phaeodactylum tricornutum.In the water-soluble extract concentration of Enteromorpha is that maximum depression effect showed postvaccinal the 8th day under 0.7g/L handled, and inhibiting rate reaches 84.8%.The upgrowth situation of Phaeodactylum tricornutum was seen Fig. 2 under the water-soluble extract of the bar Enteromorpha of variable concentrations was handled.
Claims (4)
1, a kind of preparation method of enteromorpha extract that suppresses ocean Prorocentrum and Phaeodactylum tricornutum is characterized in that, is raw material with fresh Enteromorpha, and adding distil water grinds or smashs to pieces; Add seawater again through boiling, centrifugal; Gained supernatant repeatedly centrifugal 2-3 time again is an extract with final gained liquid.
2, the method for claim 1 is characterized in that, described centrifugal condition is: 3000 rev/mins, and 20 minutes.
3, the method for claim 1 is characterized in that, described enteromorpha extract optium concentration is 0.6-1.0g/L.
4, the method for claim 1 is characterized in that, described Enteromorpha is the bar Enteromorpha.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910194502A CN101642134A (en) | 2009-08-25 | 2009-08-25 | Preparation method of enteromorpha extract capable of inhibiting prococentrum marinum and phaeodactylum tricornutum bohlin |
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| CN200910194502A CN101642134A (en) | 2009-08-25 | 2009-08-25 | Preparation method of enteromorpha extract capable of inhibiting prococentrum marinum and phaeodactylum tricornutum bohlin |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105879427A (en) * | 2016-05-09 | 2016-08-24 | 青岛海大生物集团有限公司 | Preparation method of enteromorpha biological anti-algae agent |
| CN117866773A (en) * | 2023-12-28 | 2024-04-12 | 宁波路鸣生物科技有限公司 | Carbon dioxide independent phaeodactylum tricornutum light energy autotrophic culture method |
-
2009
- 2009-08-25 CN CN200910194502A patent/CN101642134A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105879427A (en) * | 2016-05-09 | 2016-08-24 | 青岛海大生物集团有限公司 | Preparation method of enteromorpha biological anti-algae agent |
| CN117866773A (en) * | 2023-12-28 | 2024-04-12 | 宁波路鸣生物科技有限公司 | Carbon dioxide independent phaeodactylum tricornutum light energy autotrophic culture method |
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Application publication date: 20100210 |