CN102742726B - Preparation method of feed additive containing lentinan and micromolecular peptides - Google Patents
Preparation method of feed additive containing lentinan and micromolecular peptides Download PDFInfo
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- CN102742726B CN102742726B CN 201110102164 CN201110102164A CN102742726B CN 102742726 B CN102742726 B CN 102742726B CN 201110102164 CN201110102164 CN 201110102164 CN 201110102164 A CN201110102164 A CN 201110102164A CN 102742726 B CN102742726 B CN 102742726B
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Abstract
The invention discloses a preparation method of a feed additive containing lentinan and micromolecular peptides; which is characterized in that dried foot of lentinus edodes waste and an aquatic product processing waste are taken as raw materials to prepare the feed additive containing lentinan and micromolecular peptides. According to the invention, the foot of lentinus edodes waste and the aquatic product processing waste are fully recovered and utilized, and the economic benefit can be substantially increased. According to the invention, the produced aquatic product feed additive can better increase the immunization capability of the breeding animals, and the bait coefficient can be reduced.
Description
Technical field
The present invention relates to feeding additive aquatic animal processing method field, refer more particularly to a kind of preparation method who contains the feed addictive of lentinan and small-molecular peptides.
Background technology
Lentinan is the effective active composition that extracts from mushroom, main component is polysaccharide and unrighted acid, the ergosterol and the fungisterol that also contain a large amount of changed into vitamin Ds, the T cytoactive that immunologic function is arranged in but the lentinan mediator of hot water extracting stops can reduce the ability of methyl cholanthrene induced tumor.Mushroom has had strong inhibitory effects to cancer cell, is 97.5% to the inhibiting rate of small white mouse sarcoma 180, is 80% to the inhibiting rate of ehrlich carcinoma.Mushroom also contains double stranded RNA, can induce the generation interferon, has anti-virus ability.Lentinan can also improve the activity of the superoxide dismutase (SOD) of body in addition, and has the effect of removing free radical.
Peptide is a kind of biochemical substances between amino acid and protein, and it is littler than protein molecular weight, and is bigger than amino acid molecular amount, is a fragment of protein.By tens amino acid peptide bonds are continuous to aggregate into peptide down to reaching more than two, being joined with side chain by a plurality of peptides aggregates into protein again.Link to each other with peptide bond between the plural amino acid, " amino acid chain " of formation or " amino acid string " just are called peptide.Wherein, 10 peptides of forming with upper amino acid are called as polypeptide, and just are called oligopeptides by what 2 to 9 amino acid were formed, just are called small-molecular peptides or little peptide by what 2 to 4 amino acid were formed.It is reported that small-molecular peptides can directly enter in the cell with original shape by the infiltration of cell membrane, and do not need to digest again, also do not need consumes energy, thus the absorption of small-molecular peptides, conversion and utilization have efficiently, fully, characteristics completely.Small-molecular peptides has very strong biologically active, participates in the synthetic of enzyme, excites the activity of enzyme, strengthens the function of enzyme, keeps the stable of enzyme; By synthetic specific protein, produce special physiological effect.Small-molecular peptides is dual for the effect of cell, and the demand repair deficiency that namely can supplement the nutrients can improve functional status again.
Existing feeding additive aquatic animal can not improve the immunocompetence of letting animals feed mostly well, becomes the limiting factor that Aquatic Feed Processing is made.The research and development of green feeding additive aquatic animal also be into year over the developing direction of the sector, guarantee that letting animals feed green, environmental protection are the matters of utmost importance of aquaculture industry.The present invention utilizes mushroom stems leftover bits and pieces and aquatic products processing leftover bits and pieces to be raw material, form the feeding additive aquatic animal that is rich in lentinan and small-molecular peptides through fermentation, raw material draws materials itself just to have embodied turns waste into wealth, the aim of safety and environmental protection, the feed addictive that the present invention simultaneously produces can improve the immunity of letting animals feed preferably.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point of the prior art and provide a kind of technology simple, turn waste into wealth, the preparation method of the feed addictive that contains lentinan and small-molecular peptides easy to make.
The present invention realizes in the following way:
A kind of preparation method who contains the feed addictive of lentinan and small-molecular peptides is characterized in that:
Concrete steps are as follows:
One. the lentinan preparation:
(1), the mushroom stems ultramicro grinding is handled:
Get dry mushroom stems leftover bits and pieces, coarse crushing is crossed 60 mesh sieves and is got the mushroom stems meal, crosses the mushroom stems meal of 60 mesh sieves, behind 65 ℃ of forced air drying 48h, carries out ultramicro grinding again and handles;
(2), liquid preparation of producing enzyme cultivation and enzyme liquid:
Scrape 1 ring Trichoderma viride spore inoculating in the triangular flask that contains the potato fluid nutrient medium with oese, mixing is placed 28 ℃ the middle 72h of cultivation of biochemical incubator (or shaking table); Get mixed-culture medium and carry out low-speed centrifugal (or qualitative filter paper filtration), get supernatant and be required enzyme liquid;
(3), the preparation of mushroom stems saccharification liquid:
Mushroom stems powder after the ultramicro grinding of learning from else's experience carries out enzymolysis with enzyme liquid in the step (two), and the bulky grain residue of removing in the enzymolysis product namely gets mushroom stems saccharification liquid;
(4) lentinan formulations prepared from solutions:
Behind double medium filtration, gained filtrate is by standing over night AUTOMATIC ZONING, centrifugal with gained mushroom stems saccharification liquid in the step (three), and the supernatant of gained is lentinan solution;
Two. the small-molecular peptides preparation:
(1), water intaking product processing fent, remove impurity after, add water, utilize the homogenate of tissue homogenate crusher machine then;
(2), the homogenate of step () gained is added the marine low temperature alkali protease, carry out enzymolysis, obtain enzymolysis liquid behind the enzymolysis;
(3), with the enzymolysis liquid of step (two) through screen filtration, obtain filtered fluid after the filtration;
(4), the filtered fluid of step (three) is carried out centrifugal filtration with the butterfly centrifugal machine, get supernatant;
(5), with step (fours') supernatant, carry out second ultrafiltration and concentrate, make small-molecular peptides solution, and it is standby to add stabilizing agent;
Three. contain the preparation of the feed addictive of lentinan and small-molecular peptides:
Step 1 gained lentinan solution is fully mixed with step 2 gained small-molecular peptides solution, and lentinan solution is 1: 5～10 with small-molecular peptides liquor capacity ratio, carries out spray-drying, namely gets the feed addictive that contains lentinan and small-molecular peptides after the drying.
The preparation method of described potato fluid nutrient medium is with peeling potatoes, is cut into piece and adds water, boils 30min, uses filtered through gauze, and gained filtrate adds glucose, supplies water to 100ml, the triangular flask of packing into.
Protein in the described marine low temperature alkali protease energy hydrolyzed aquatic products processing fent discharges small-molecular peptides, and the grease that hydrolysis simultaneously produces can be used for Feed Manufacturing.
Can be used for Feed Manufacturing after the described upper strata grease drying with the centrifugal filtered fluid of butterfly centrifugal machine.
The present invention utilizes lentinan can improve superoxide dismutase activity and other various biological functions, has immunoregulation effect, also can activate the characteristics of the BA of small-molecular peptides simultaneously.Small-molecular peptides also has the various biological effect, is used for the effect that feed addictive can not only be brought into play lentinan and small-molecular peptides self, and a collaborative effect that strengthens is arranged simultaneously each other, can improve immunocompetence significantly.The nutritive value of lentinan and small-molecular peptides self also can improve feed efficiency.
Mushroom stems leftover bits and pieces and aquatic products processing leftover bits and pieces be all devalued processing generally, have greatly wasted resource, and the present invention fully recycles it, have improved economic benefit widely.The feeding additive aquatic animal that the present invention simultaneously produces can improve the immunocompetence of letting animals feed well, reduces feed coefficient.
The invention has the advantages that:
1, production technology is simple, and is easy to operate.
2, reclaim wasted resource effectively.
3, improve the immunocompetence of letting animals feed, reduce feed coefficient.
The specific embodiment
The specific embodiment of the invention now is described in detail in detail:
A kind of preparation method who contains the feed addictive of lentinan and small-molecular peptides is characterized in that:
Concrete steps are as follows:
One. the lentinan preparation:
(1), the mushroom stems ultramicro grinding is handled:
Get dry mushroom stems leftover bits and pieces, coarse crushing is crossed 60 mesh sieves and is got the mushroom stems meal, crosses the mushroom stems meal of 60 mesh sieves, behind 65 ℃ of forced air drying 48h, carries out ultramicro grinding again and handles;
(2), liquid preparation of producing enzyme cultivation and enzyme liquid:
Scrape 1 ring Trichoderma viride spore inoculating in the triangular flask that contains the potato fluid nutrient medium with oese, mixing is placed 28 ℃ the middle 72h of cultivation of biochemical incubator (or shaking table); Get mixed-culture medium and carry out low-speed centrifugal (or qualitative filter paper filtration), get supernatant and be required enzyme liquid;
(3), the preparation of mushroom stems saccharification liquid:
Mushroom stems powder after the ultramicro grinding of learning from else's experience carries out enzymolysis with enzyme liquid in the step (two), and the bulky grain residue of removing in the enzymolysis product namely gets mushroom stems saccharification liquid;
(4) lentinan formulations prepared from solutions:
Behind double medium filtration, gained filtrate is by standing over night AUTOMATIC ZONING, centrifugal with gained mushroom stems saccharification liquid in the step (three), and the supernatant of gained is lentinan solution;
Two. the small-molecular peptides preparation:
(1), water intaking product processing fent, remove impurity after, add water, utilize the homogenate of tissue homogenate crusher machine then;
(2), the homogenate of step () gained is added the marine low temperature alkali protease, carry out enzymolysis, obtain enzymolysis liquid behind the enzymolysis;
(3), with the enzymolysis liquid of step (two) through screen filtration, obtain filtered fluid after the filtration;
(4), the filtered fluid of step (three) is carried out centrifugal filtration with the butterfly centrifugal machine, get supernatant;
(5), with step (fours') supernatant, carry out second ultrafiltration and concentrate, make small-molecular peptides solution, and it is standby to add stabilizing agent;
Three. contain the preparation of the feed addictive of lentinan and small-molecular peptides:
Step 1 gained lentinan solution is fully mixed with step 2 gained small-molecular peptides solution, and lentinan solution is 1: 5～10 with small-molecular peptides liquor capacity ratio, carries out spray-drying, namely gets the feed addictive that contains lentinan and small-molecular peptides after the drying.
The preparation method of described potato fluid nutrient medium is with peeling potatoes, is cut into piece and adds water, boils 30min, uses filtered through gauze, and gained filtrate adds glucose, supplies water to 100ml, the triangular flask of packing into.
Protein in the described marine low temperature alkali protease energy hydrolyzed aquatic products processing fent discharges small-molecular peptides, and the grease that hydrolysis simultaneously produces can be used for Feed Manufacturing.
Can be used for Feed Manufacturing after the described upper strata grease drying with the centrifugal filtered fluid of butterfly centrifugal machine.
The present invention utilizes lentinan can improve superoxide dismutase activity and other various biological functions, has immunoregulation effect, also can activate the characteristics of the BA of small-molecular peptides simultaneously.Small-molecular peptides also has the various biological effect, is used for the effect that feed addictive can not only be brought into play lentinan and small-molecular peptides self, simultaneously a collaborative humidification is arranged each other, can improve immunocompetence significantly.The nutritive value of lentinan and small-molecular peptides self also can improve feed efficiency.
Mushroom stems leftover bits and pieces and aquatic products processing leftover bits and pieces be all devalued processing generally, have greatly wasted resource, and the present invention fully recycles it, have improved economic benefit widely.The feeding additive aquatic animal that the present invention simultaneously produces can improve the immunocompetence of letting animals feed well, reduces feed coefficient.
Specific embodiment one:
One. the lentinan preparation
(1), the mushroom stems ultramicro grinding is handled:
Get dry mushroom stems leftover bits and pieces 1000g, coarse crushing is crossed 60 mesh sieves and is got the mushroom stems meal, crosses the mushroom stems meal of 60 mesh sieves, behind 65 ℃ of forced air drying 48h, with 2kgh
-1Charging rate handle carrying out ultramicro grinding, the operating pressure of pulverizer is 1.0Mpa, feed pressure 0.3Mpa, tolerance is 1.8m
3.min
-1
(2), liquid preparation of producing enzyme cultivation and enzyme liquid:
Scrape 1 ring Trichoderma viride spore inoculating in the triangular flask that contains the potato fluid nutrient medium with oese, mixing is placed 28 ℃ the middle 72h of cultivation of biochemical incubator (or shaking table); Get mixed-culture medium and carry out low-speed centrifugal (or qualitative filter paper filtration), get supernatant and be required enzyme liquid;
(3), the preparation of mushroom stems saccharification liquid:
Mushroom stems after the ultramicro grinding of learning from else's experience is carried out enzymolysis with enzyme liquid in the step (two), described after ultramicro grinding mushroom stems and the enzyme liquid mass volume ratio in the step (two) be 1kg: 150ml, hydrolysis temperature is 28 ℃, the pH value is 4.5; Enzymolysis product namely gets mushroom stems saccharification liquid through removing the bulky grain residue;
(4) lentinan formulations prepared from solutions:
Behind double medium filtration, gained filtrate is by standing over night AUTOMATIC ZONING, centrifugal with gained mushroom stems saccharification liquid in the step (three), and the supernatant of gained is lentinan solution;
Two. the small-molecular peptides preparation:
(1), water intaking product processing fent, remove the 2000g that weighs behind the impurity, add water, utilize the homogenate of tissue homogenate crusher machine then;
(2), the homogenate of step () gained is added 5000U/kg marine low temperature alkali protease, carry out enzymolysis, enzymolysis control reaction temperature is at 18 ℃, and reaction pH is 6.8, and enzymolysis obtained enzymolysis liquid after 4 days;
(3), with the enzymolysis liquid of step (two) through screen filtration, obtain filtered fluid after the filtration;
(4), the filtered fluid of step (three) is carried out centrifugal filtration with the butterfly centrifugal machine, get supernatant;
(5), with step (fours') supernatant, carry out second ultrafiltration and concentrate, make small-molecular peptides solution, and it is standby to add stabilizing agent;
Three. contain the preparation of the feed addictive of lentinan and small-molecular peptides:
Step 1 gained lentinan solution is fully mixed with step 2 gained small-molecular peptides solution, and lentinan solution is 1: 5 with small-molecular peptides liquor capacity ratio, carries out spray-drying, namely gets the feed addictive that contains lentinan and small-molecular peptides after the drying.
Specific embodiment two:
One. the lentinan preparation
(1), the mushroom stems ultramicro grinding is handled:
Get dry mushroom stems leftover bits and pieces 1000g, coarse crushing is crossed 60 mesh sieves and is got the mushroom stems meal, crosses the mushroom stems meal of 60 mesh sieves, behind 65 ℃ of forced air drying 48h, with 2kgh
-1Charging rate handle carrying out ultramicro grinding, the operating pressure of pulverizer is 1.0Mpa, feed pressure 0.3Mpa, tolerance is 1.8m
3.min
-1
(2), liquid preparation of producing enzyme cultivation and enzyme liquid:
Scrape 1 ring Trichoderma viride spore inoculating in the triangular flask that contains the potato fluid nutrient medium with oese, mixing is placed 28 ℃ the middle 72h of cultivation of biochemical incubator (or shaking table); Get mixed-culture medium and carry out low-speed centrifugal (or qualitative filter paper filtration), get supernatant and be required enzyme liquid;
(3), the preparation of mushroom stems saccharification liquid:
Mushroom stems powder after the ultramicro grinding of learning from else's experience carries out enzymolysis with enzyme liquid in the step (two), described after ultramicro grinding mushroom stems and the enzyme liquid mass volume ratio in the step (two) be 1kg: 300ml, hydrolysis temperature is 30 ℃, the pH value is 5.0; Enzymolysis product namely gets mushroom stems saccharification liquid through removing the bulky grain residue;
(4) lentinan formulations prepared from solutions:
Behind double medium filtration, gained filtrate is by standing over night AUTOMATIC ZONING, centrifugal with gained mushroom stems saccharification liquid in the step (three), and the supernatant of gained is lentinan solution;
Two. the small-molecular peptides preparation:
(1), water intaking product processing fent, remove the 2000g that weighs behind the impurity, add water, utilize the homogenate of tissue homogenate crusher machine then;
(2), the homogenate of step () gained is added 7000U/kg marine low temperature alkali protease, carry out enzymolysis, enzymolysis control reaction temperature is at 20 ℃, and reaction pH is 7.0, and enzymolysis obtained enzymolysis liquid after 4 days;
(3), with the enzymolysis liquid of step (two) through screen filtration, obtain filtered fluid after the filtration;
(4), the filtered fluid of step (three) is carried out centrifugal filtration with the butterfly centrifugal machine, get supernatant;
(5), with step (fours') supernatant, carry out second ultrafiltration and concentrate, make small-molecular peptides solution, and it is standby to add stabilizing agent;
Three. contain the preparation of the feed addictive of lentinan and small-molecular peptides:
Step 1 gained lentinan solution is fully mixed with step 2 gained small-molecular peptides solution, and lentinan solution is 1: 7 with small-molecular peptides liquor capacity ratio, carries out spray-drying, namely gets the feed addictive that contains lentinan and small-molecular peptides after the drying.
Specific embodiment three:
One. the lentinan preparation
(1), the mushroom stems ultramicro grinding is handled:
Get dry mushroom stems leftover bits and pieces 1000g, coarse crushing is crossed 60 mesh sieves and is got the mushroom stems meal, crosses the mushroom stems meal of 60 mesh sieves, behind 65 ℃ of forced air drying 48h, with 2kgh
-1Charging rate handle carrying out ultramicro grinding, the operating pressure of pulverizer is 1.0Mpa, feed pressure 0.3Mpa, tolerance is 1.8m
3.min
-1
(2), liquid preparation of producing enzyme cultivation and enzyme liquid:
Scrape 1 ring Trichoderma viride spore inoculating in the triangular flask that contains the potato fluid nutrient medium with oese, mixing is placed 28 ℃ the middle 72h of cultivation of biochemical incubator (or shaking table); Get mixed-culture medium and carry out low-speed centrifugal (or qualitative filter paper filtration), get supernatant and be required enzyme liquid;
(3), the preparation of mushroom stems saccharification liquid:
Mushroom stems powder after the ultramicro grinding of learning from else's experience carries out enzymolysis with enzyme liquid in the step (two), described after ultramicro grinding mushroom stems and the enzyme liquid mass volume ratio in the step (two) be 1kg: 500ml, hydrolysis temperature is 33 ℃, the pH value is 5.5; Enzymolysis product namely gets mushroom stems saccharification liquid through removing the bulky grain residue;
(4) lentinan formulations prepared from solutions:
Behind double medium filtration, gained filtrate is by standing over night AUTOMATIC ZONING, centrifugal with gained mushroom stems saccharification liquid in the step (three), and the supernatant of gained is lentinan solution;
Two. the small-molecular peptides preparation:
(1), water intaking product processing fent, remove the 2000g that weighs behind the impurity, add water, utilize the homogenate of tissue homogenate crusher machine then;
Two), the homogenate of step () gained is added 8000U/kg marine low temperature alkali protease, carry out enzymolysis, enzymolysis control reaction temperature is at 22 ℃, and reaction pH is 7.3, and enzymolysis obtained enzymolysis liquid after 4 days;
(3), with the enzymolysis liquid of step (two) through screen filtration, obtain filtered fluid after the filtration;
(4), the filtered fluid of step (three) is carried out centrifugal filtration with the butterfly centrifugal machine, get supernatant;
(5), with step (fours') supernatant, carry out second ultrafiltration and concentrate, make small-molecular peptides solution, and it is standby to add stabilizing agent;
Three. contain the preparation of the feed addictive of lentinan and small-molecular peptides:
Step 1 gained lentinan solution is fully mixed with step 2 gained small-molecular peptides solution, and lentinan solution is 1: 10 with small-molecular peptides liquor capacity ratio, carries out spray-drying, namely gets the feed addictive that contains lentinan and small-molecular peptides after the drying.
Experimental data of the present invention:
Adopt the feed addictive of embodiment one method preparation to carry out following test, as seen feed addictive of the present invention can improve that animal organism is disease-resistant, the immunocompetence of anti-poor environment, improve survival rate, reduced feed coefficient, reached good economic benefits.
Test the effect test of the feed addictive nursing of adding the preparation of embodiment one method 1 seedling stage
Somewhere plant establishes three and handles experiment, 110 days every mouthful of ponds of feeding period;
Result of the test sees Table 1:
Table 1 Penaeus Vannmei adds the feed addictive test of embodiment one preparation seedling stage
As can be seen from Table 1: the weight according to 3% and 1% is added the feed addictive of embodiment 1 preparation in the Penaeus Vannmei feed to, adds 3% feed addictive test group and has improved 20.4% than contrast composition motility rate, and feed coefficient has reduced by 0.19; Add 1% feed addictive test group and improved 14.5% than contrast composition motility rate, feed coefficient has reduced by 0.13.It is 17.5% that test group on average improves survival rate than control group; This test is through variance analysis, and test group and control group difference reach the level of signifiance (P＜0.05).
Test 2 somewhere plants, the weight according to 5% and 3% is added the feed addictive of embodiment 1 preparation in the Penaeus Vannmei feed to, respectively establishes three groups, and feeding period is 110 days.
Result of the test sees Table 2:
Table 2 Penaeus Vannmei adds the feed addictive test of embodiment 1 preparation seedling stage
As can be seen from Table 2: the weight according to 5% and 3% is added the feed addictive of embodiment one method preparation in the Penaeus Vannmei feed to, the survival rate of test group improves 20.82% and 17.51% than control group respectively, and feed coefficient has descended 0.176 and 0.123 respectively.
Test 3 tests in Ningde three all Australia large yellow croaker nursery carry out, select cement pit for use, 150 square metres of areas, dark 1.5 meters of pond, 1.0 meters of the depth of waters, feeding period is 165 days.
Result of the test sees Table 3:
Table 3 large yellow croaker is added the feed addictive culture experiment of embodiment 1 preparation seedling stage
As can be seen from Table 3: the weight according to 5% and 3% is added the feed addictive of embodiment 1 preparation in the large yellow croaker feed in seedling stage to, raises fry 45 days, and 5% additive test group is formed motility rate than contrast and improved 15.3%, and feed coefficient has descended 0.87; 3% additive test group is formed motility rate than contrast and is improved 11.1%, and feed coefficient has descended 0.72.This test is through variance analysis, and test group and control group difference reach the level of signifiance (P＜0.05).
To test 4 lefteye flounder fish tests and carry out in Ningde plant, select cement pit for use, 120 square metres of areas, dark 1.2 meters of pond, 0.9 meter of the depth of water. feeding period is 120 days, adds with 5% and 3% ratio, tests.
Result of the test sees Table 4:
Table 4 lefteye flounder fish is raised the feed addictive test of adding embodiment 1 preparation
As can be seen from Table 4: 5% additive test group descends 1.33 than control group feed coefficient, and survival rate has improved 12.6%; 3% additive test group descends 1.08 than control group feed coefficient, and survival rate has improved 6.0%.
Test 5 tests and carry out in Ningde sweetfish plant, select for use at cement pit, 150 square metres of areas, the pond is dark 1.2,0.9 meter of the depth of water, feeding period is 160 days.Weight according to 5% and 3% is added, and tests.
Result of the test sees Table 5:
Table 5 sweetfish is raised the additive test of adding the preparation of embodiment one method
As can be seen from Table 5: 5% additive test group descends 0.37,3% additive test group than control group feed coefficient decline 0.20 than control group feed coefficient; 5% additive improves 10.9%, 3% additive than contrast composition motility rate and forms motility rate raising 7.5% than contrast.
Claims (4)
1. preparation method who contains the feed addictive of lentinan and small-molecular peptides is characterized in that:
Concrete steps are as follows:
One. the lentinan preparation:
(1), the mushroom stems ultramicro grinding is handled:
Get dry mushroom stems leftover bits and pieces, coarse crushing is crossed 60 mesh sieves and is got the mushroom stems meal, crosses the mushroom stems meal of 60 mesh sieves, behind 65 ℃ of forced air drying 48h, carries out ultramicro grinding again and handles;
(2), liquid preparation of producing enzyme cultivation and enzyme liquid:
Scrape 1 ring Trichoderma viride spore inoculating in the triangular flask that contains the potato fluid nutrient medium with oese, mixing, be placed in 28 ℃ biochemical incubator or the shaking table and cultivate 72h: get that mixed-culture medium carries out low-speed centrifugal or qualitative filter paper filters, get supernatant and be required enzyme liquid:
(3), the preparation of mushroom stems saccharification liquid:
Mushroom stems powder after the ultramicro grinding of learning from else's experience carries out enzymolysis with enzyme liquid in the step (two), and the bulky grain residue of removing in the enzymolysis product namely gets mushroom stems saccharification liquid:
(4) lentinan formulations prepared from solutions:
Behind double medium filtration, gained filtrate is by standing over night AUTOMATIC ZONING, centrifugal with gained mushroom stems saccharification liquid in the step (three), and the supernatant of gained is lentinan solution:
Two. the small-molecular peptides preparation:
(1), water intaking product processing fent, remove impurity after, add water, utilize the homogenate of tissue homogenate crusher machine then;
(2), the homogenate of step () gained is added the marine low temperature alkali protease, carry out enzymolysis, obtain enzymolysis liquid behind the enzymolysis;
(3), with the enzymolysis liquid of step (two) through screen filtration, obtain filtered fluid after the filtration;
(4), the filtered fluid of step (three) is carried out centrifugal filtration with the butterfly centrifugal machine, get supernatant;
(5), with step (fours') supernatant, carry out second ultrafiltration and concentrate, make small-molecular peptides solution, and it is standby to add stabilizing agent;
Three. contain the preparation of the feed addictive of lentinan and small-molecular peptides;
Step 1 gained lentinan solution is fully mixed with step 2 gained small-molecular peptides solution, and lentinan solution is 1: 5～10 with small-molecular peptides liquor capacity ratio, carries out spray-drying, namely gets the feed addictive that contains lentinan and small-molecular peptides after the drying.
2. a kind of preparation method who contains the feed addictive of lentinan and small-molecular peptides according to claim 1, it is characterized in that: the preparation method of described potato fluid nutrient medium is with peeling potatoes, be cut into piece and add water, boil 30min, use filtered through gauze, gained filtrate adds glucose, supplies water to 100ml, the triangular flask of packing into.
3. a kind of preparation method who contains the feed addictive of lentinan and small-molecular peptides according to claim 1, it is characterized in that: the protein in the described marine low temperature alkali protease energy directionally hydrolyzing disposal from fishery product processing, discharge small-molecular peptides, the grease that hydrolysis simultaneously produces can be used for Feed Manufacturing.
4. a kind of preparation method who contains the feed addictive of lentinan and small-molecular peptides according to claim 1 is characterized in that: can be used for Feed Manufacturing after the described upper strata grease drying with the centrifugal filtered fluid of butterfly centrifugal machine.
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| CN103614301B (en) * | 2013-11-22 | 2015-08-26 | 浙江工业大学 | Produce the aspergillus niger of lytic enzyme and the application in preparation mushroom zymolyte thereof |
| CN104004813B (en) * | 2014-06-12 | 2017-12-29 | 北京林业大学 | A kind of preparation of mushroom biologically active peptide |
| CN107581616A (en) * | 2017-08-18 | 2018-01-16 | 武汉华士特工业生物技术开发有限公司 | A kind of method that protein from lentinus edodes and mushroom oligosaccharide are synchronously prepared using mushroom |
| CN108339109A (en) * | 2018-01-17 | 2018-07-31 | 浙江海洋大学 | A kind of clam immunoloregulation polypeptide tablet and preparation method |
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| CN101828647B (en) * | 2010-06-03 | 2013-09-04 | 程永发 | Health care feedstuff additive for animals |
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