CN101897347A - Plant-based bacteriostat, preparation method thereof and application thereof - Google Patents
Plant-based bacteriostat, preparation method thereof and application thereof Download PDFInfo
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- CN101897347A CN101897347A CN2010102241057A CN201010224105A CN101897347A CN 101897347 A CN101897347 A CN 101897347A CN 2010102241057 A CN2010102241057 A CN 2010102241057A CN 201010224105 A CN201010224105 A CN 201010224105A CN 101897347 A CN101897347 A CN 101897347A
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Abstract
The invention discloses a plant-based bacteriostat, a preparation method and application thereof. The plant-based bacteriostat of the invention comprises active extracts of myoporum bontioides leaves. The plant-based bacteriostat of the invention has an inhibitory effect on a plurality of germs, particularly rice blast, sheath blight or bacterial blight. The plant-based bacteriostat of the invention has the advantages of high efficiency, low toxicity, environmental-friendliness, easy degradation, high germ resistance and the like and can be used as an ideal green pesticide.
Description
Technical field
The present invention relates to phytopathogen and suppress the field, be specifically related to a kind of plant-based bacteriostat and its production and application.
Background technology
Rice blast (rice blast), banded sclerotial blight (sheath blight), bacterial leaf-blight (bacterial blight) are worldwide three big diseases.The medicament that China's paddy rice three big disease controls are at present used all is a chemical agent, though control efficiency is good, but the farm chemical ingredients in the paddy is too high, human body is damaged, make people's health easily change various illnesss, easily cause the immunity degradation of each function of human body, the edible for a long time too high rice of remains of pesticide, the generation of the various cancers of easier hidden danger.In addition, pesticide resistance has appearred in some areas, causes that people pay close attention to greatly.Therefore, the suitable control paddy rice three big disease new drugs of research are significant.
The research of the development and use of at present relevant plant bacteriostatic agent mainly contains: Chinese patent application CN2005100863079, CN200510086762.9 disclose a kind of plant polyphenol bacteriostatic agent and the composite bacteriostatic agent of the many branches of a plant species and preparation method thereof respectively; The CN93110345.2 Chinese patent application discloses a kind of method of extracting bacteriostatic agent and extract bacteriostatic agent from the Chinese parasol tree bark from this plant; 200810137637.X number Chinese patent application the plant medical agent that utilizes pine tree leaf, pine needle, Huang Tongye, Da Ye to prevent and treat rotten neck for feedstock production is a kind of by leaf, wild anise, peach leaves, camphortree leaf is disclosed; 200910025838.5 number Chinese patent application disclose and utilized the botanical pesticide of Chinese scholar tree plant for the inhibition rice blast of feedstock production.The employing various plants extract that these patents have, the needs that have extract than pure component, have just at single phytopathogen, particularly do not see simultaneously the main disease of paddy rice had and suppress effect.
Myoporaceae (Myoporaceae) plant comprises three genus: desert wood belongs to (Eremophila), Myoporum (Myoporum) and Bontia L.It is genus maximum in the Myoporaceae that the desert belongs to, and the plant of having identified at present has 209 kinds.About 32 kinds altogether of Myoporums are dicotyledon.This platymiscium mainly is distributed in the ground such as Mauritius of Asia (for example China and Japan), Oceania (for example Australia and New Zealand), Pacific Hawaiian Islands and the Indian Ocean.China has only bitter sill indigo plant (M.bontioides) a kind of, originates in the seashore in Guangdong and Guangxi Provinces, Fujian and Taiwan.
Summary of the invention
The objective of the invention is to according to existing be that the fungistatic effect that exists in the bacteriostatic agent of raw material is single, to extract floristics many with the plant, weak points such as extract components purity requirement height, the plant-based bacteriostat that provides a kind of efficient, low toxicity, environmentally friendly, easy degraded, germ to be difficult for producing resistance, and this bacteriostatic agent has for three big diseases of hazard rice production and suppresses effect significantly.
Another purpose of the present invention is to provide the preparation method of above-mentioned plant-based bacteriostat.
A further object of the invention is to provide the application of above-mentioned plant-based bacteriostat.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
Plant-based bacteriostat of the present invention is a primary raw material with bitter sill indigo plant, and wherein main active ingredient is 5,7-dihydroxy flavanones.Wherein, the extraction solvent of the blue leaf extract of described bitter sill is one or more the mixture in water, methyl alcohol, ethanol, acetone, butanols, ethyl acetate, carrene, n-hexane, cyclohexane, the benzinum.
As a kind of preferred version, the described bacteriostatic agent of plant-based bacteriostat of the present invention is made up of following components in percentage by mass: the blue extract 12.11～15.63% of bitter sill, emulsifier 0.73～1.04%, water 83.33～87.16%.
Plant-based bacteriostat of the present invention can be prepared into aqua, aqueous emulsion or microemulsion.
In the said components, described emulsifier is tween, this Pan's series or other agricultural emulsifier.
The preparation method of plant-based bacteriostat of the present invention comprises the steps:
(1) with the blue leaf dried and crushed of bitter sill, sieve, obtain bitter sill blue powder end;
(2) bitter sill blue powder end is put into extracted still, add and extract solvent soaking, filter, repeat to extract 3 times, merging filtrate reclaims solvent, obtains solid content and be 40.00～66.67% the blue extract of bitter sill;
(3) the blue extract of bitter sill, auxiliary agent and solvent are added in the stirred tank in proportion, fully mix, obtain plant-based bacteriostat.
As a kind of preferred version, among the above-mentioned preparation method, sieve described in the step (1) to crossing 40 mesh sieves.
As a kind of preferred version, among the above-mentioned preparation method, the addition of extracting solvent described in the step (2) is 2～4 times of bitter sill blue powder end; Soak time is 7 days.
Plant-based bacteriostat of the present invention can be used to prepare the bacteriostatic agent that suppresses the plant resource germ, especially to the inhibition best results of rice blast fungus, Rhizoctonia solani Kuhn or Xanthomonas oryzae.In addition, owing to contain multiple terpenes, flavonoids, phenolic compound in the bitter sill basket, these compounds can carry out condensation, neutralization reaction with groups such as sulfydryl, amido, ammonia, eliminate the stink substance that is free in the environment.Contained active ingredient is inhibited equally to fruit germ in postpartum.Therefore plant-based bacteriostat of the present invention can also be used for deodorizing and fresh-keeping.
Compared with prior art, the present invention has following beneficial effect:
(1) plant-based bacteriostat of the present invention contains polytype compounds such as flavones, flavonoids, terpene, sterol, and these compounds all have bacteriostatic activity; Plant-based bacteriostat fungistatic effect of the present invention is remarkable, is easy to decompose in environment, and pathogen is difficult for producing resistance, can well utilize multi-component synergistic effect in the extract, reaches the effect that effectively suppresses the various plants pathogen simultaneously;
(2) in the plant-based bacteriostat of the present invention preparation, the bitter sill indigo plant of used raw material is grown in semiarid and the coastal area, can artificial cultivation, and aboundresources, of many uses, and extract obtains easily;
(3) plant-based bacteriostat of the present invention is effective to the inhibition of the main disease of paddy rice, does not see at present both at home and abroad paddy rice three big main diseases are all had the plant-based bacteriostat of fungistatic effect.
Embodiment
Further explain the present invention below in conjunction with embodiment, but embodiment does not do any type of qualification to the present invention.
Embodiment 1
5Kg puts into the extraction still with the blue leaf powder of bitter sill, the 95 volume % ethanol that add 10Kg soaked 7 days under the room temperature, filtered, so repeat 3 times, merging filtrate, concentrating under reduced pressure, getting solid content is the blue extract 1.365kg of bitter sill of 40wt%, the Tween-80 that adds 0.080kg, fully stir, add water 9.698kg again, promptly make the emulsion of the bacteriostatic agent of 5wt%.
Embodiment 2
5kg puts into the extraction still with the blue leaf powder of bitter sill, the 70 volume % ethanol that add 10kg, soaked 7 days under the room temperature, so extract merging filtrate 3 times, concentrating under reduced pressure, getting solid content is the blue extract 1.159kg of bitter sill of 65wt%, adds the Tween-80 of 0.06kg, fully stirs, add water 6.307kg again, promptly make the bacteriostatic agent emulsion of 10wt%.
Embodiment 3
5kg puts into the extraction still with the blue leaf powder of bitter sill, add 10kg acetone, soaked 7 days under the room temperature, so extract 3 times, merging filtrate, concentrating under reduced pressure, getting solid content is the blue extract 1.250kg of bitter sill of 40wt%, adds the Tween-80 of 0.07kg, 0.01kg Si Pan-20, fully stir, add water 8.872kg again, promptly make the bacteriostatic agent emulsion of 5wt%.
Embodiment 4
5kg extracts in the still with the blue leaf powder of bitter sill, the 50 volume % ethanol that add 10kg, soaked 7 days under the room temperature, so extract merging filtrate 3 times, concentrating under reduced pressure, getting solid content is the blue extract 1.105kg of the bitter sill of 65wt%, adds the Tween-60 of 0.057kg, fully stirring, add water 6.012kg again, promptly make the bacteriostatic agent emulsion of 10wt%.
Embodiment 5 Toxicity Determination results
1. the raw material and the test of pesticide effectiveness
1.1 experimental pharmacy
Get dry bitter sill blue powder end 5kg and put into the extraction still, add the 70 volume % ethanol of 10Kg, soaked 7 days under the room temperature, so extract 3 times, merging filtrate, concentrating under reduced pressure gets solid content and is the blue extract 1.365kg of bitter sill of 40wt%, and is standby in 4 ℃ of refrigerators preservations.
1.2 the dull and stereotyped experiment of band poison
Under aseptic condition, getting quality respectively is 0.225g (0.112g), 0.45g (0.168g), 0.675g (0.224g), 0.900g (0.280g) and the said extracted liquid of 1.125g (0.336g) in conical flask, the medium of pouring thawing again into is to 45ml, pour in the culture dish of 3 above diameter 9cm after fully shaking up rapidly, obtaining mass concentration is 0.20% (0.1%), 0.40% (0.15%), 0.60% (0.20%), 0.80% (0.25%), 1.0% (0.3%) flat board, drying the back is the bacterium piece of 5mm at dull and stereotyped central authorities access diameter, each concentration is done 3 repetitions, coordinative solvent compares, place 28 ℃ of constant incubators to cultivate, after cultivating 3d, adopt the right-angled intersection method to measure colony diameter, calculate inhibiting rate.Result such as following table 1:(annotate: the data in the above-mentioned bracket are the experimental data of rice blast fungus)
The blue extract of the bitter sill of table 1 suppresses paddy rice three big disease flat-plate experimental results
The blue ethanol extract of the bitter sill of test shows all has tangible bacteriostasis to paddy rice three serious diseases evil pathogen under isolated condition.Especially the paddy rice rice blast fungus had strong inhibition activity, EC
50Be 0.12%, to the EC of rice banded sclerotial blight bacterium and paddy rice bacterial leaf spot bacterium
50Be respectively 0.35% and 0.36%.
Claims (10)
1. a plant-based bacteriostat is characterized in that described bacteriostatic agent comprises the blue leaf extract of bitter sill.
2. plant-based bacteriostat according to claim 1, the extraction solvent that it is characterized in that the blue leaf extract of described bitter sill are one or more the mixture in water, methyl alcohol, ethanol, acetone, butanols, ethyl acetate, carrene, n-hexane, cyclohexane, the benzinum.
3. plant-based bacteriostat according to claim 2 is characterized in that described bacteriostatic agent is made up of following components in percentage by mass: the blue extract 12.11～15.63% of bitter sill, emulsifier 0.73～1.04%, water 83.33～87.16%.
4. plant-based bacteriostat according to claim 3 is characterized in that described emulsifier is tween, this Pan's series or other agricultural emulsifier.
5. the preparation method of any described plant-based bacteriostat of claim in the claim 1～4 is characterized in that comprising the steps:
(1) with the blue leaf dried and crushed of bitter sill, sieve, obtain bitter sill blue powder end;
(2) bitter sill blue powder end is put into the extraction still, added and extract solvent soaking, filter, repeat to extract 3 times, merging filtrate reclaims solvent, obtains bitter sill indigo plant extract;
(3) the blue extract of bitter sill, auxiliary agent and solvent are added in the stirred tank in proportion, fully mix, obtain plant-based bacteriostat.
6. according to the preparation method of the described plant-based bacteriostat of claim 5, it is characterized in that sieving described in the step (1) into crossing 40 mesh sieves.
7. according to the preparation method of the described plant-based bacteriostat of claim 5, the addition that it is characterized in that the solvent of extraction described in the step (2) is 2～4 times of bitter sill blue powder end; Soak time is 7 days.
8. the application of any described plant-based bacteriostat of claim in suppressing the plant resource germ in the claim 1～4.
9. the application of described plant-based bacteriostat according to Claim 8 is characterized in that described plant resource germ is rice blast fungus, Rhizoctonia solani Kuhn or Xanthomonas oryzae.
In the claim 1～4 any described plant-based bacteriostat of claim deodorizing and fresh-keeping in application.
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| CN2010102241057A CN101897347A (en) | 2010-07-09 | 2010-07-09 | Plant-based bacteriostat, preparation method thereof and application thereof |
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| CN2010102241057A CN101897347A (en) | 2010-07-09 | 2010-07-09 | Plant-based bacteriostat, preparation method thereof and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102989025A (en) * | 2012-12-28 | 2013-03-27 | 青岛信锐德科技有限公司 | Camphor tree leaf mosquito repellent air fresher and preparation method |
| CN106400310A (en) * | 2016-08-30 | 2017-02-15 | 华南农业大学 | Nano-fiber anti-microbial fruit-vegetable preservative film with porous structure, and preparation method and application |
| CN106966472A (en) * | 2017-04-28 | 2017-07-21 | 中国海洋大学 | A kind of preparation method of sewage bacteriophage inhibitor and application |
| CN108619020A (en) * | 2018-06-13 | 2018-10-09 | 广州喜澳化妆品有限公司 | A kind of facial mask and preparation method thereof containing Myoporum bontiodes extract |
| CN109553565A (en) * | 2018-11-05 | 2019-04-02 | 华南农业大学 | Two kinds of new alkaloids and preparation method thereof and the application in the antibacterial agent for preparing anti-MRSA |
| CN114369323A (en) * | 2022-01-21 | 2022-04-19 | 华南农业大学 | Plant-source sunlight-driven efficient antibacterial and antiviral multilayer composite nanofiber protective material and preparation method and application thereof |
| CN116395734A (en) * | 2023-03-01 | 2023-07-07 | 浙江大学 | Method for preparing nano copper oxide particles by using hibiscus flower extract, product and application |
-
2010
- 2010-07-09 CN CN2010102241057A patent/CN101897347A/en active Pending
Non-Patent Citations (8)
| Title |
|---|
| 何衍彪等: "苦槛蓝提取物对芒果炭疽菌和香蕉尖孢镰刀菌的抑制作用 ", 《广东农业科学》 * |
| 何衍彪等: "苦槛蓝提取物对芒果炭疽菌和香蕉尖孢镰刀菌的抑制作用", 《广东农业科学》, no. 06, 10 June 2006 (2006-06-10) * |
| 谷文祥等: "苦槛蓝提取物对小菜蛾的生物活性 ", 《应用生态学报》 * |
| 谷文祥等: "苦槛蓝提取物对小菜蛾的生物活性", 《应用生态学报》, no. 07, 18 July 2004 (2004-07-18) * |
| 邓业成等: "红树植物提取物的杀螨活性试验 ", 《农药》 * |
| 邓业成等: "红树植物提取物的杀螨活性试验", 《农药》, no. 08, 10 August 2007 (2007-08-10) * |
| 阳振等: "红树植物甲醇提取物的抑菌活性研究 ", 《热带海洋学报》 * |
| 阳振等: "红树植物甲醇提取物的抑菌活性研究", 《热带海洋学报》, no. 01, 30 January 2007 (2007-01-30) * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102989025A (en) * | 2012-12-28 | 2013-03-27 | 青岛信锐德科技有限公司 | Camphor tree leaf mosquito repellent air fresher and preparation method |
| CN106400310A (en) * | 2016-08-30 | 2017-02-15 | 华南农业大学 | Nano-fiber anti-microbial fruit-vegetable preservative film with porous structure, and preparation method and application |
| CN106966472A (en) * | 2017-04-28 | 2017-07-21 | 中国海洋大学 | A kind of preparation method of sewage bacteriophage inhibitor and application |
| CN108619020A (en) * | 2018-06-13 | 2018-10-09 | 广州喜澳化妆品有限公司 | A kind of facial mask and preparation method thereof containing Myoporum bontiodes extract |
| CN108619020B (en) * | 2018-06-13 | 2022-02-01 | 广州喜澳化妆品有限公司 | A facial mask containing herba Ixeritis Sonchifoliae extract and its preparation method |
| CN109553565A (en) * | 2018-11-05 | 2019-04-02 | 华南农业大学 | Two kinds of new alkaloids and preparation method thereof and the application in the antibacterial agent for preparing anti-MRSA |
| CN109553565B (en) * | 2018-11-05 | 2022-04-05 | 华南农业大学 | Two new alkaloids, preparation method thereof and application thereof in preparation of MRSA-resistant antibacterial agent |
| CN114369323A (en) * | 2022-01-21 | 2022-04-19 | 华南农业大学 | Plant-source sunlight-driven efficient antibacterial and antiviral multilayer composite nanofiber protective material and preparation method and application thereof |
| CN114369323B (en) * | 2022-01-21 | 2022-10-25 | 华南农业大学 | Plant-source sunlight-driven efficient antibacterial and antiviral multilayer composite nanofiber protective material and preparation method and application thereof |
| CN116395734A (en) * | 2023-03-01 | 2023-07-07 | 浙江大学 | Method for preparing nano copper oxide particles by using hibiscus flower extract, product and application |
| CN116395734B (en) * | 2023-03-01 | 2024-06-11 | 浙江大学 | Method for preparing nano copper oxide particles by using hibiscus flower extract, product and application |
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Application publication date: 20101201 |