CN104604950A - Momordica grosvenori extracted algistat, as well as preparation method and application thereof - Google Patents
Momordica grosvenori extracted algistat, as well as preparation method and application thereof Download PDFInfo
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- CN104604950A CN104604950A CN201510007221.6A CN201510007221A CN104604950A CN 104604950 A CN104604950 A CN 104604950A CN 201510007221 A CN201510007221 A CN 201510007221A CN 104604950 A CN104604950 A CN 104604950A
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- momordica grosvenori
- algae
- inhibiting agent
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- leaching liquor
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Abstract
The invention discloses a momordica grosvenori extracted algistat, as well as a preparation method and application thereof. The preparation method comprises the following steps: a, grinding the momordica grosvenori into momordica grosvenori powder; b, mixing the momordica grosvenori powder with alcohol and water to prepare a momordica grosvenori suspension; c, concentrating the momordica grosvenori suspension to prepare the momordica grosvenori extracted algistat. The momordica grosvenori extraction algistat prepared through the method can be used for effectively inhibiting the growth of algae.
Description
Technical field
The present invention relates to algae-inhibiting agent, particularly, relate to a kind of Momordica grosvenori leaching liquor algae-inhibiting agent and its preparation method and application.
Background technology
Alga eruption is a kind of Disasters frequently appearing at seawater and freshwater waters at present, is called red tide, in fresh water, is called wawter bloom in ocean.The kind of the algae that can form wawter bloom mainly in Cyanophyta and Chlorophyta.In eutrophication poisons in freshwater, with the microcystic aeruginosa of Cyanophyta and anabena the most common.
Prevent from algal bloom and control wawter bloom becoming the task of top priority.Algae-inhibiting agent because being eased to make wawter bloom by fast onset alga-inhibiting action, thus becomes one of method of wawter bloom emergency processing.Algae-inhibiting agent can be divided into physical algae-inhibiting agent, chemically algae-inhibiting agent and biochemical algae-inhibiting agent, the character of these algae-inhibiting agents is as follows: 1) physical algae-inhibiting agent: effect of algae restraint is remarkable, but need the long period to take effect, and the metal ion such as iron, aluminium can be introduced in water body, lowly still have pollution though pollute; 2) chemically algae-inhibiting agent: instant effect, has toxicity equally to aquatile while killing algae, often there is the hidden danger of secondary pollution; 3) biological algae-inhibiting agent: low toxicity, low cost, therefore becomes the research direction of current water environment treatment technology.
Existing biological algae-inhibiting agent otherwise be inhibiting rate below 50%, or be the large usage quantity of algae-inhibiting agent.
Summary of the invention
The object of this invention is to provide a kind of Momordica grosvenori leaching liquor algae-inhibiting agent and preparation method thereof, the Momordica grosvenori leaching liquor algae-inhibiting agent obtained by the method can suppress the growth of algae effectively.
To achieve these goals, the invention provides a kind of preparation method of Momordica grosvenori leaching liquor algae-inhibiting agent, preparation method comprises:
A, by Momordica grosvenori pulverize make Grosvenor Momordica Fruit;
B, by Grosvenor Momordica Fruit, alcohol and water mixing in obtained Momordica grosvenori suspension;
C, by concentrated obtained for described Momordica grosvenori suspension Momordica grosvenori leaching liquor algae-inhibiting agent.
Preferably, the particle diameter of Grosvenor Momordica Fruit is not less than 150 orders.
Preferably, in stepb, relative to the Grosvenor Momordica Fruit of 1 weight portion, the consumption of described water is 15-25 weight portion.
Preferably, in stepb, mixing meets the following conditions: incorporation time is 2-4 days, and mixing temperature is 15-35 DEG C.
Preferably, in step c, with the gross weight of described Momordica grosvenori leaching liquor algae-inhibiting agent for benchmark, the content of described Grosvenor Momordica Fruit is 8-12 % by weight.
Present invention provides a kind of Momordica grosvenori leaching liquor algae-inhibiting agent, Momordica grosvenori leaching liquor algae-inhibiting agent is obtained by the preparation of above-mentioned method.
Present invention also offers a kind of according to the application of above-mentioned Momordica grosvenori leaching liquor algae-inhibiting agent in the growth suppressing algae.
By technique scheme, Grosvenor Momordica Fruit and water mixing, by by after Momordica grosvenori pulverizing, then soak by the present invention, finally concentrated just obtained Momordica grosvenori leaching liquor algae-inhibiting agent.This algae-inhibiting agent adopt pure natural substance preparation and environmental pollution is little, the inhibiting rate of this algae-inhibiting agent to algae is high and consumption is little simultaneously.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Accompanying drawing explanation
Accompanying drawing is used to provide a further understanding of the present invention, and forms a part for specification, is used from explanation the present invention, but is not construed as limiting the invention with embodiment one below.In the accompanying drawings:
Fig. 1 is the statistical chart of the algae cell density in each part microcystic aeruginosa liquid in test example 1;
Fig. 2 is the result statistical chart of the inhibiting rate-incubation time in test example 1;
Fig. 3 is the result statistical chart of the inhibiting rate-incubation time in test example 3;
Fig. 4 is the statistical chart of the algae cell density in each part microcystic aeruginosa liquid in test example 4.
Embodiment
Below the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The invention provides a kind of preparation method of Momordica grosvenori leaching liquor algae-inhibiting agent, preparation method comprises:
A, by Momordica grosvenori pulverize make Grosvenor Momordica Fruit;
B, by Grosvenor Momordica Fruit, alcohol and water mixing in obtained Momordica grosvenori suspension;
C, by concentrated obtained for described Momordica grosvenori suspension Momordica grosvenori leaching liquor algae-inhibiting agent.
In preparation method provided by the invention, the particle diameter of Grosvenor Momordica Fruit can be selected in wide scope, but in order to the effect making the Momordica grosvenori leaching liquor algae-inhibiting agent obtained have more excellent suppression algal grown, preferably, the particle diameter of Grosvenor Momordica Fruit is not less than 150 orders.
In preparation method provided by the invention, the consumption of Grosvenor Momordica Fruit and water can be selected in wide scope, but in order to the effect making the Momordica grosvenori leaching liquor algae-inhibiting agent obtained have more excellent suppression algal grown, preferably, in stepb, relative to the Grosvenor Momordica Fruit of 1 weight portion, the consumption of described water is 15-25 weight portion.
In preparation method provided by the invention, the actual conditions of mixing can be selected in wide scope, but in order to the effect making the Momordica grosvenori leaching liquor algae-inhibiting agent obtained have more excellent suppression algal grown, preferably, in stepb, mixing meets the following conditions: incorporation time is 2-4 days, and mixing temperature is 15-35 DEG C.
In the present invention, solid-liquid weight ratio in Momordica grosvenori leaching liquor algae-inhibiting agent can be selected in wide scope, but in order to the effect making the Momordica grosvenori leaching liquor algae-inhibiting agent obtained have more excellent suppression algal grown, preferably, in step c, with the gross weight of described Momordica grosvenori leaching liquor algae-inhibiting agent for benchmark, the content of described Grosvenor Momordica Fruit is 8-12 % by weight.
Present invention provides a kind of Momordica grosvenori leaching liquor algae-inhibiting agent, Momordica grosvenori leaching liquor algae-inhibiting agent is obtained by the preparation of above-mentioned method.
Present invention also offers a kind of according to the application of above-mentioned Momordica grosvenori leaching liquor algae-inhibiting agent in the growth suppressing algae.
Below will be described the present invention by embodiment.In following examples, Momordica grosvenori is the commercially available product in the large pharmacy of Wuhu common people; Microcystic aeruginosa liquid: microcystic aeruginosa PCC7806 (purchased from Inst. of Hydrobiology, Chinese Academy of Sciences's algae kind storehouse) is carried out cultivation in BG-11 medium (concrete composition is in table 1) and obtains microcystic aeruginosa liquid, condition of culture is: intensity of illumination is 4000lx, Light To Dark Ratio is 12h:12h, temperature is 24-26 DEG C, every day, the number of times of shake was 3-5 time, cultivated and obtained microcystic aeruginosa liquid after 7 days.
Table 1
Embodiment 1
A, Momordica grosvenori to be smashed, cross 150 object sieves and obtain Grosvenor Momordica Fruit;
B, at 25 DEG C, Grosvenor Momordica Fruit is soaked in 3 days obtained Momordica grosvenori suspensions in water; Wherein, the mass ratio of Grosvenor Momordica Fruit and the aqueous solution is 1:20.
C, by concentrated for above-mentioned Momordica grosvenori suspension until obtain Momordica grosvenori leaching liquor algae-inhibiting agent A1 when solid-liquid weight ratio is 1:10.
Comparative example 1
A, Momordica grosvenori to be smashed, cross 150 object sieves and obtain Grosvenor Momordica Fruit;
B, at 4 DEG C, Grosvenor Momordica Fruit to be soaked in water 3 days, then filtering with microporous membrane degerming (miillpore filter diameter 0.22 μm produces house: upper Haixing County is sub-) obtained Momordica grosvenori suspension at 25 DEG C; Wherein, the mass ratio of Grosvenor Momordica Fruit and the aqueous solution is 1:20.
C, by concentrated for above-mentioned Momordica grosvenori suspension until obtain Momordica grosvenori leaching liquor algae-inhibiting agent B1 when solid-liquid weight ratio is 1:10.
Comparative example 2
A, Momordica grosvenori to be smashed, cross 150 object sieves and obtain Grosvenor Momordica Fruit;
B, at 100 DEG C, Grosvenor Momordica Fruit is soaked in water and boils 10 minutes and sterilizing 20min obtains Momordica grosvenori suspension at 121 DEG C; Wherein, the mass ratio of Grosvenor Momordica Fruit and the aqueous solution is 1:20.
C, by concentrated for above-mentioned Momordica grosvenori suspension until obtain Momordica grosvenori leaching liquor algae-inhibiting agent B1 when solid-liquid weight ratio is 1:10.
Test example 1
The Momordica grosvenori leaching liquor algae-inhibiting agent A1 of difference amount is added to microcystic aeruginosa liquid, and (dilution makes the initial algae cell density of microcystic aeruginosa liquid be 5 × 10
5cells/ml) make the mass fraction of the Momordica grosvenori leaching liquor in microcystic aeruginosa liquid be respectively 0,0.04%, 0.08%, 0.12%, 0.16% and 0.2% in, calculate the algae cell density in a microcystic aeruginosa liquid every 24h, Continuous plus 7 days.Concrete outcome is shown in Fig. 1.As shown in Figure 1, Momordica grosvenori leaching liquor algae-inhibiting agent A1 has excellent Developing restraint effect to algae; And the increase of the concentration of the Momordica grosvenori leaching liquor algae-inhibiting agent A1 in microcystic aeruginosa liquid, inhibiting rate is larger; The time of Momordica grosvenori leaching liquor algae-inhibiting agent A1 in microcystic aeruginosa liquid is longer, and inhibiting rate is larger.
Test example 2
By the microcystic aeruginosa liquid of five parts of normal growths, (algae cell density is 2.2 × 10
6cells/ml) be diluted to 100%, 80%, 60%, 40%, 20% of former algae liquid respectively, in these five parts of microcystic aeruginosa liquid, then add the mass fraction that Momordica grosvenori leaching liquor algae-inhibiting agent A1 makes it in respective microcystic aeruginosa liquid is 2%.Then respectively 3h, 6h, after 1 day, 2 days and 3 days with counting method of blood cell statistics algae cell density and calculate inhibiting rate, concrete outcome is shown in Fig. 3.
As shown in Figure 3, Momordica grosvenori leaching liquor algae-inhibiting agent A1 is 1.2 × 10 to algae cell density
6cells/ml-6 × 10
6the microcystic aeruginosa liquid of cells/ml all has excellent inhibitory action.
Test example 3
By blake bottle each in test example 2 at the 4th day centrifugal segregation culture fluid, then the fresh BG-11 medium of 50ml is respectively added, be placed in incubator and cultivate, condition of culture is: intensity of illumination is 4000lx, Light To Dark Ratio is 12h:12h, temperature is 24-26 DEG C, every day, the number of times of shake was 3-5 time, algae cell density (that day changing culture fluid is designated as 0d) is calculated respectively at 1d, 2d, 3d blood counting chamber, wherein with the microcystic aeruginosa liquid of 50ml as a control group, algal control rate (IR)=(1-N/N
0) × 100%, N is the algae cell density of processed group, N
0for the algae cell density of control group.Concrete inhibiting rate the results are shown in Figure 3.
As shown in Figure 3, Momordica grosvenori leaching liquor algae-inhibiting agent acted on microcystic aeruginosa after four days, also had delay effect to the suppression of microcystic aeruginosa.
Test example 4
Get the microcystic aeruginosa liquid of 50ml in the conical flask of 100ml, then in this conical flask, a certain amount of Chinese herbal medicine algae-inhibiting agent is added as processed group, then algae cell density is calculated by blood cell plate counting method afterwards 5 days and 9 days respectively, wherein with the microcystic aeruginosa liquid of 50ml as a control group, algal control rate (IR)=(1-N/N0) × 100%, N is the algae cell density of processed group, N
0for the algae cell density of control group.
Momordica grosvenori leaching liquor algae-inhibiting agent A1, B1 and B2 are added to respectively in different microcystic aeruginosa liquid that (initial density of frustule is 2.6 × 10
6cells/mL) carry out cultivating (mass fraction of Momordica grosvenori leaching liquor algae-inhibiting agent in microcystic aeruginosa liquid is 0.3%), survey the inhibiting rate after cultivating, wherein, condition of culture is: intensity of illumination is 4000lx, Light To Dark Ratio is 12h:12h, temperature is 24-26 DEG C, and every day, the number of times of shake was 3-5 time.Wherein, blank group is not with the microcystic aeruginosa liquid of Momordica grosvenori leaching liquor algae-inhibiting agent.Concrete inhibiting rate the results are shown in Figure 4.
As shown in Figure 4, the effect of algae restraint of Momordica grosvenori leaching liquor algae-inhibiting agent provided by the invention and bacterium have nothing to do, and this Momordica grosvenori leaching liquor algae-inhibiting agent has heat endurance.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible combination.
In addition, also can be combined between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (7)
1. a preparation method for Momordica grosvenori leaching liquor algae-inhibiting agent, is characterized in that, described preparation method comprises:
A, by Momordica grosvenori pulverize make Grosvenor Momordica Fruit;
B, by described Grosvenor Momordica Fruit and water mixing with obtained Momordica grosvenori suspension;
C, by concentrated obtained for described Momordica grosvenori suspension Momordica grosvenori leaching liquor algae-inhibiting agent.
2. preparation method according to claim 1, wherein, the particle diameter of described Grosvenor Momordica Fruit is not less than 150 orders.
3. preparation method according to claim 1, wherein, in stepb, relative to the Grosvenor Momordica Fruit of 1 weight portion, the consumption of described water is 15-25 weight portion.
4. preparation method according to claim 1, wherein, in stepb, described mixing meets the following conditions: incorporation time is 2-4 days, and mixing temperature is 15-35 DEG C.
5. preparation method according to claim 1, wherein, in step c, with the gross weight of described Momordica grosvenori leaching liquor algae-inhibiting agent for benchmark, the content of described Grosvenor Momordica Fruit is 8-12 % by weight.
6. a Momordica grosvenori leaching liquor algae-inhibiting agent, is characterized in that, described Momordica grosvenori leaching liquor algae-inhibiting agent is obtained by the preparation of the method described in any one in claim 1-5.
7. the application of Momordica grosvenori leaching liquor algae-inhibiting agent according to claim 6 in the growth suppressing algae.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954643A (en) * | 2017-04-26 | 2017-07-18 | 南华大学 | The method that algistatic activity composition prepares algae-inhibiting agent is extracted from grape pip |
| CN112544640A (en) * | 2020-12-15 | 2021-03-26 | 安徽师范大学 | Algae inhibitor derived from sugarcane peel and preparation method thereof |
-
2015
- 2015-01-07 CN CN201510007221.6A patent/CN104604950B/en active Active
Non-Patent Citations (1)
| Title |
|---|
| 艾丽萍，高顺: "中药罗汉果对小鼠急性毒性和致突变性研究", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954643A (en) * | 2017-04-26 | 2017-07-18 | 南华大学 | The method that algistatic activity composition prepares algae-inhibiting agent is extracted from grape pip |
| CN112544640A (en) * | 2020-12-15 | 2021-03-26 | 安徽师范大学 | Algae inhibitor derived from sugarcane peel and preparation method thereof |
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