CN109892344B - Terrestrial plant source algistat and preparation method thereof - Google Patents
Terrestrial plant source algistat and preparation method thereof Download PDFInfo
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- CN109892344B CN109892344B CN201910139490.6A CN201910139490A CN109892344B CN 109892344 B CN109892344 B CN 109892344B CN 201910139490 A CN201910139490 A CN 201910139490A CN 109892344 B CN109892344 B CN 109892344B
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- lactic acid
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- SVPKNMBRVBMTLB-UHFFFAOYSA-N 2,3-dichloronaphthalene-1,4-dione Chemical compound C1=CC=C2C(=O)C(Cl)=C(Cl)C(=O)C2=C1 SVPKNMBRVBMTLB-UHFFFAOYSA-N 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 104
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 52
- 239000004310 lactic acid Substances 0.000 claims abstract description 52
- 238000002386 leaching Methods 0.000 claims abstract description 30
- 241000218033 Hibiscus Species 0.000 claims abstract description 22
- 235000005206 Hibiscus Nutrition 0.000 claims abstract description 21
- 235000007185 Hibiscus lunariifolius Nutrition 0.000 claims abstract description 21
- 241000192710 Microcystis aeruginosa Species 0.000 claims abstract description 20
- 241000196324 Embryophyta Species 0.000 claims abstract description 14
- 241000195493 Cryptophyta Species 0.000 claims description 18
- 241000628997 Flos Species 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 9
- 238000010298 pulverizing process Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 5
- 244000130592 Hibiscus syriacus Species 0.000 claims description 4
- 235000018081 Hibiscus syriacus Nutrition 0.000 claims description 4
- 239000003112 inhibitor Substances 0.000 claims description 3
- 230000005764 inhibitory process Effects 0.000 abstract description 17
- 239000003619 algicide Substances 0.000 abstract description 7
- 241000205585 Aquilegia canadensis Species 0.000 abstract 2
- 241001570521 Lonicera periclymenum Species 0.000 description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 230000000694 effects Effects 0.000 description 9
- 239000003627 allelochemical Substances 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 6
- 108010049746 Microcystins Proteins 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- 241000245240 Lonicera Species 0.000 description 2
- 244000167230 Lonicera japonica Species 0.000 description 2
- 235000017617 Lonicera japonica Nutrition 0.000 description 2
- 241000192701 Microcystis Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229940061720 alpha hydroxy acid Drugs 0.000 description 2
- 150000001280 alpha hydroxy acids Chemical class 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- KVZLHPXEUGJPAH-UHFFFAOYSA-N 2-oxidanylpropanoic acid Chemical compound CC(O)C(O)=O.CC(O)C(O)=O KVZLHPXEUGJPAH-UHFFFAOYSA-N 0.000 description 1
- 241000192542 Anabaena Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- SRUWWOSWHXIIIA-UKPGNTDSSA-N Cyanoginosin Chemical compound N1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](C)[C@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C(=C)N(C)C(=O)CC[C@H](C(O)=O)N(C)C(=O)[C@@H](C)[C@@H]1\C=C\C(\C)=C\[C@H](C)[C@@H](O)CC1=CC=CC=C1 SRUWWOSWHXIIIA-UKPGNTDSSA-N 0.000 description 1
- 240000003450 Mallotus philippensis Species 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000192497 Oscillatoria Species 0.000 description 1
- 241001528187 Paliurus Species 0.000 description 1
- 241000200821 Platypodium Species 0.000 description 1
- 241000951473 Schizonepeta Species 0.000 description 1
- 230000004099 anaerobic respiration Effects 0.000 description 1
- 230000001775 anti-pathogenic effect Effects 0.000 description 1
- 210000001099 axilla Anatomy 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
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- 238000007865 diluting Methods 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 108010067094 microcystin Proteins 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a terrestrial plant source algistat and a preparation method thereof, wherein the algistat is hibiscus flower lactic acid extract and honeysuckle flower lactic acid extract. Compared with the prior art, the invention adopts lactic acid to extract the effective components of terrestrial plants to prepare the algicide, the inhibition rate of the prepared hibiscus flower lactic acid leaching liquor algicide high-concentration treatment group on microcystis aeruginosa cells at the 4 th day reaches more than 80%, and the inhibition rate of the honeysuckle flower lactic acid leaching liquor algicide high-concentration treatment group on microcystis aeruginosa cells at the 4 th day reaches more than 90%.
Description
Technical Field
The invention belongs to an algistat and a preparation method thereof, in particular to a terrestrial plant source algistat and a preparation method thereof.
Background
In southern rural areas in China, blue-green algae widely exists in water bodies such as ponds, riversides and the like, presently, microcystins, anabaena and oscillatoria are found in algae capable of generating microcystins (microcystins), and most of the water bloom found in China is microcystins. The harm of microcystin generated by microcystis is great, and the discovery of an efficient algae inhibiting method with small ecological risk is a breakthrough point of the current treatment.
Disclosure of Invention
The invention aims to solve the technical problem 1 to provide a terrestrial plant source algicide with good ecological safety and used for microcystis.
The invention aims to solve the technical problem 2, namely a preparation method of the terrestrial plant source algistat.
The technical scheme for solving the technical problem of the invention is as follows: the terrestrial plant source algae inhibitor is prepared from a hibiscus syriacus lactic acid leaching liquor and a honeysuckle lactic acid leaching liquor.
The preferred algistat is lactic acid leaching liquor of honeysuckle
The hibiscus syriacus lactic acid leaching liquor is prepared by the following method:
pulverizing flos Hibisci powder to less than 200 mesh, mixing pulverized flos Hibisci with lactic acid (g/ml) at ratio of 1:5-8, leaching on shaking table at room temperature for 3-5 days, and collecting supernatant.
The honeysuckle lactic acid leaching liquor is prepared by the following method:
pulverizing flos Lonicerae to less than 200 mesh, mixing pulverized flos Lonicerae with lactic acid (g/ml) at a ratio of 1:5-8, leaching on a shaking table at room temperature for 3-5 days, and collecting supernatant.
The algae is microcystis aeruginosa.
The density of the algae is 1-9 x 106One/ml.
The Hibiscus flower of the invention is native in China, and is a dried flower of Hibiscus (Hibiscus syfiacus L.) of Hibiscus of Malvaceae. The mallotus philippinensis, schizonepeta, paliurus, platypodium and the like belong to malvaceae hibiscus deciduous shrubs or small arbors, are 2-6 m high, have axilla of flowers with single-leaf growth, have short stems, have white, purple, red and other colors, and are bell-shaped flowers. Honeysuckle (Lonicera japonica Thunb) is a common traditional Chinese medicine, is a dried flower bud of Lonicera Linn of Caprifoliaceae Lonicera and Lonicera japonica Thunb, has long application history, has the effects of clearing heat and removing toxicity, cooling and dispersing wind heat, and has strong anti-pathogenic microorganism effect.
Although the utilization of higher plant allelochemicals for inhibiting algae is one of the current research hotspots, the allelochemicals are organic compounds and are insoluble in water; the organic solvent is used after the allelochemicals are separated and extracted by adopting the organic solvent and is volatilized to be used, the organic solvent is toxic to the environment, and the allelochemicals volatilized to the organic solvent are used in practice only after being dissolved, so that the problem of solving the solubility of the allelochemicals becomes the bottleneck of effective application of the allelochemicals.
Lactic acid (lactnic acid), known as 2-hydroxypropionic acid (2-hydroxypropionic acid), is an alpha-hydroxy acid (AHA) with a molecular weight of 90.08 and a molecular formula of C3H6O 3. Lactic acid is a natural organic acid, can be mixed with water at will, is colorless, clear or yellowish viscous liquid, and has no odor and slight acid. Mainly from the synthesis of lactic acid bacteria, and also anaerobic respiration products of animals and few plants. Lactic acid has wide application in food industry, can be used as preservative, food additive and sour agent, and yoghourt and lactic acid beverage contain abundant lactic acid, so that the ecological algistat prepared by promoting allelochemicals to dissolve and synergize by using lactic acid has high environmental safety.
Compared with the prior art, the invention adopts lactic acid to extract the effective components of terrestrial plants to prepare the algicide, the inhibition rate of the prepared hibiscus flower lactic acid leaching liquor algicide high-concentration treatment group on microcystis aeruginosa cells at the 4 th day reaches more than 80%, and the inhibition rate of the honeysuckle flower lactic acid leaching liquor algicide high-concentration treatment group on microcystis aeruginosa cells at the 4 th day reaches more than 90%.
Drawings
FIG. 1 shows the effect of different concentrations of lactic acid on the growth of Microcystis aeruginosa.
FIG. 2 is a graph showing the effect of water extract of hibiscus flowers obtained in example 7 on microcystis aeruginosa at different concentrations.
FIG. 3 shows the effect of different concentrations of the water extract of honeysuckle flower prepared in example 8 on Microcystis aeruginosa.
FIG. 4 shows the percent inhibition (%) of micro-encapsulated aeruginosa by lactic acid leach liquor of hibiscus flowers prepared in example 2 at different concentrations.
FIG. 5 shows the percent inhibition (%) of Microcystis aeruginosa by lactic acid leaching solution of honeysuckle flower prepared in example 5 with different concentrations.
Detailed Description
The present invention will be described in detail with reference to examples.
Microcystis aeruginosa (FACHB-942) as a strain used in the invention is purchased from Wuhan aquatic organism research institute of Chinese academy of sciences, and is cultured by using BG-11 medium. The culture conditions are as follows: the temperature is 25 ℃, the illumination intensity is 4000lx, the light dark period is 12 h: culturing for 12h for one week to make Microcystis aeruginosa reach logarithmic growth phase, and diluting with BG-11 culture medium to initial algae density of 3.6 × 106one/mL.
Hibiscus flower and honeysuckle flower are purchased from pharmacy of common people in Wenhu city.
Lactic acid is analytically pure lactic acid.
The calculation formula of the microcystis aeruginosa inhibition rate is as follows:
wherein: IR-inhibition, N-number of algal cells in experimental group, N0-number of algal cells in control group.
Example 1:
pulverizing flos Hibisci powder to less than 200 mesh, mixing pulverized flos Hibisci with lactic acid (g/ml) at ratio of 1:5, leaching on shaking table at room temperature for 3 days, and collecting supernatant.
Example 2:
pulverizing flos Hibisci powder to less than 200 mesh, mixing pulverized flos Hibisci with lactic acid (g/ml) at ratio of 1:6, leaching on shaking table at room temperature for 4 days, and collecting supernatant.
Example 3:
pulverizing flos Hibisci powder to less than 200 mesh, mixing pulverized flos Hibisci with lactic acid (g/ml) at ratio of 1:8, leaching on shaking table at room temperature for 5 days, and collecting supernatant.
Example 4
Pulverizing flos Lonicerae to less than 200 mesh, mixing pulverized flos Lonicerae with lactic acid (g/ml) at a ratio of 1:5, leaching on a shaker at room temperature for 3 days, and collecting supernatant.
Example 5
Pulverizing flos Lonicerae to less than 200 mesh, mixing pulverized flos Lonicerae with lactic acid (g/ml) at a ratio of 1:6, leaching on a shaker at room temperature for 4 days, and collecting supernatant.
Example 6
Pulverizing flos Lonicerae to less than 200 mesh, mixing pulverized flos Lonicerae with lactic acid (g/ml) at a ratio of 1:8, leaching on a shaker at room temperature for 5 days, and collecting supernatant.
Example 7:
the procedure was as in example 2 except that the solvent used was water.
Example 8:
the procedure is as in example 4 except that the solvent used is water.
Example 9:
adding Microcystis aeruginosa in logarithmic growth phase into 250mL conical flask, adding BG-11 medium to make the initial algae density of each group at 3.2 × 106cells/mL, the volume of the solution is 100mL, pure lactic acid, the lactic acid leaching solution of hibiscus flower prepared in example 2, the lactic acid leaching solution of honeysuckle prepared in example 5, the water leaching solution of hibiscus flower prepared in example 7 and the water leaching solution of honeysuckle prepared in example 8 are respectively added into each conical flask, so that the final concentrations of the lactic acid, the lactic acid leaching solution of hibiscus flower or honeysuckle and the water leaching solution of hibiscus flower or honeysuckle in each conical flask are respectively 25 muL/L, 50 muL/L, 75 muL/L and 100 muL, control groups without inhibitor are set, 3 parallels are set for each group, algae cells are counted by a cell counting microscope every 24h, and the number of cells is continuously measured for 5 d.
The culture conditions for each group were: the illumination intensity is 4000lx, the light-dark ratio is 12 h: 12h at 25 +/-1 ℃. Shaking 3-5 times daily.
As shown in fig. 1, 2 and 3: the pure lactic acid has stronger inhibiting effect on microcystis aeruginosa under the algae density, the maximum inhibiting rate is that the highest concentration group (100 mu L/L) appears at the 5 th day and reaches 66.49 percent, the hibiscus flower water extract hardly influences the microcystis aeruginosa under the action concentration, the honeysuckle water extract shows certain inhibiting effect under the same concentration with the lactic acid, and the maximum inhibiting rate is that the highest concentration group (100 mu L/L) appears at the 5 th day and reaches 62.53 percent
TABLE 1 inhibition ratio (%)% of lactic acid leaching solution of hibiscus flower and honeysuckle flower with different concentrations on Microcystis aeruginosa
As can be seen from table 1, fig. 4 and fig. 5, the inhibition rates of the lactic acid leaching solution of hibiscus syriacus and the lactic acid leaching solution of honeysuckle on microcystis aeruginosa both increased with the time, and showed significant concentration effects. Although the water extract of hibiscus flower has almost no algae inhibition effect, the lactic acid extract of hibiscus flower has an inhibition effect at low concentration, the inhibition rate of the high-concentration treatment group of the lactic acid extract of hibiscus flower on microcystis aeruginosa cells at day 4 reaches 83.17%, and the inhibition rate of the high-concentration treatment group of the lactic acid extract of honeysuckle flower at day 4 reaches 94.47%, which is obviously better than that of single lactic acid or honeysuckle flower algae inhibition, so that the extraction of lactic acid can promote the dissolution of the algae inhibition components of honeysuckle flower and hibiscus flower on one hand, and the lactic acid and the algae inhibition components exert a synergistic algae inhibition effect on the other hand.
Claims (4)
1. The terrestrial plant source algistat is characterized in that: the algae inhibitor is lactic acid leaching liquor of hibiscus flower.
2. The terrestrial plant-derived algistat according to claim 1, characterized in that:
the algae is microcystis aeruginosa.
3. The terrestrial plant-derived algistat according to claim 2, characterized in that:
the density of the algae is 1-9 × 106One/ml.
4. The terrestrial plant-derived algistat according to claim 1, which is prepared by the following method:
the hibiscus syriacus lactic acid leaching liquor is prepared by the following method:
pulverizing flos Hibisci powder to less than 200 mesh, mixing pulverized flos Hibisci with lactic acid (g/ml) at ratio of 1:5-8, leaching on shaking table at room temperature for 3-5 days, and collecting supernatant.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191993A (en) * | 2015-09-22 | 2015-12-30 | 安徽师范大学 | Plant source algicide and preparation method thereof |
| CN106879645A (en) * | 2017-01-20 | 2017-06-23 | 安徽师范大学 | A kind of high-efficiency plant source algae-inhibiting agent and preparation method thereof |
| CN108770883A (en) * | 2018-06-21 | 2018-11-09 | 安徽师范大学 | A kind of environmental-friendly algae-inhibiting agent |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191993A (en) * | 2015-09-22 | 2015-12-30 | 安徽师范大学 | Plant source algicide and preparation method thereof |
| CN106879645A (en) * | 2017-01-20 | 2017-06-23 | 安徽师范大学 | A kind of high-efficiency plant source algae-inhibiting agent and preparation method thereof |
| CN108770883A (en) * | 2018-06-21 | 2018-11-09 | 安徽师范大学 | A kind of environmental-friendly algae-inhibiting agent |
Non-Patent Citations (2)
| Title |
|---|
| "植物源溶藻化合物研究进展";过七根 等;《天然产物研究与开发》;20170503(第29期);摘要，第1230-1235页第2节 * |
| "洋葱乳酸浸提液对铜绿微囊藻的抑制效应";文竹 等;《卫生研究》;20180930;第47卷(第5期);摘要，第1.2节，第826页左栏第2段 * |
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