CN102687733B - Black locust plant acaricide, preparation method and application thereof - Google Patents
Black locust plant acaricide, preparation method and application thereof Download PDFInfo
- Publication number
- CN102687733B CN102687733B CN201210137309.6A CN201210137309A CN102687733B CN 102687733 B CN102687733 B CN 102687733B CN 201210137309 A CN201210137309 A CN 201210137309A CN 102687733 B CN102687733 B CN 102687733B
- Authority
- CN
- China
- Prior art keywords
- extract
- locust tree
- locust
- black locust
- plant acaricide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a black locust plant acaricide, a preparation method and an application of the black locust plant acaricide, wherein the acaricide comprises the following components in percentage by weight: 10-40% of black locust branch extract, 10-40% of black locust stem extract, 5-35% of black locust leaf extract, 5-35% of black locust flower extract and 20-60% of distilled water; the black locust branch extract, the black locust stem extract, the black locust leaf extract and the black locust flower extract are extractum obtained by steps of grinding black locust branches, stems, leaves and flowers after being dried and then performing decompressing concentration. The black locust plant acaricide has high control efficiency for Tetranychus cinnabarinus, can be directly used for preventing and treating the insects, has the advantages of harmlessness, no pollution and no residual toxins, and avoids the damage of chemical pesticide.
Description
Technical field
That the present invention relates to is a kind of preparation method of technical field of pesticide, specifically, is a kind of preparation with locust tree extract preparation control Tetranychus cinnabarinus and its preparation method and application.
Background technology
Tetranychus cinnabarinus (Tetranychus cinnabarinus) is that China distributes the most extensively, occurs the most serious a kind of agricultural evil mite, its individuality is little, breeding soon, many, the strong adaptability of year generation, endangering very seriously, is one of the pest monoid that is difficult to control.At present, the control of this harmful mite mainly relies on chemical agent, as the long-term use that sprays this medicament such as organic phosphates and pyrethrin pesticide not only caused the pesticide resistance of mite class, and non-target organism has also been produced to adverse effect.Botanical pesticide have broad spectrum activity, mechanism of action uniqueness, to natural enemy disturb less, to vertebrate without overt toxicity, in environment degraded rapidly, aboundresources, the advantage such as renewable, be the ideal chose of control evil mite.And acaricidal activity material is extensively present in natural various plants, the comprehensive advantage that can utilize plant secondary substance to show completely, makes up numerous defects that current existing miticide exists.Therefore go out to send searching structural material from plant source natural product, become one of focus of novel miticide research and development.
Locust tree (Robinia pseudoacacia L.), has another name called acacia, is the perennial deciduous tree of pulse family robinia.Worldwide extensively distribute, approximately 20 kinds, the robinia plant whole world, China's cultivation is now introduced a fine variety comparatively general, aboundresources, biomass is large; Locust tree is widely used in the aspects such as feed, greening, water and soil conservation, is also a kind of reasonable nectariferous plant and construction material.In locust tree, contain various active composition, have antibacterial, diuresis, anti-inflammatory, spasmolysis, heat-clearing, phlegm-dispelling functions, some active components are used to improve capillary microcirculation, the ancillary drug for the treatment of acute and chronic nephritis, uremic, cystitis and gynecological disease.Bibliographical information locust tree kind active component is mainly robinin, agglutinin and multiple types Flavonoid substances etc.
Find through prior art literature search, Chen Jiao etc. are entitled as in " research and development of natural products " (05 phase in 2002) in the article of " the Screening of Antibacterial Activities research of 58 Plant Extracts to Plasmopara viticola " and have reported that locust tree extract is inhibited to Plasmopara viticola.In retrieval, also find, China Patent Publication No. CN101147499A, patent name " by the preparation method of water solution with locust stems and leaves extraction for controlling cucumber powdery mildew ", this patent is mentioned, and can prevent and treat powdery mildew of cucumber with the water extract of locust stems and leaves.Further in retrieval, do not find that locust tree extract is for preventing and treating the research report of Tetranychus cinnabarinus.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of method of controlling Tetranychus cinnabarinus aqua of preparing with locust tree extract is provided, make it there is higher control efficiency to Tetranychus cinnabarinus, can be directly used in this kind of insect pest of control, and have nuisanceless, pollution-freely and without the feature of residual hazard, avoid the harm of chemical pesticide.
The present invention relates to a kind of locust tree plant acaricide, formed by following component and weight percent content: locust tree branch extract 10 ~ 40%, locust tree stem extraction 10 ~ 40%, leaf of locust extract 5 ~ 35%, Pagoda Flower extract 5 ~ 35% and distilled water 20 ~ 60%.
Described locust tree branch extract, locust tree stem extraction, leaf of locust extract, Pagoda Flower extract, be respectively locust tree branch, stem, leaf, flower to be clayed into power after drying, then the medicinal extract that adopts the method for reduced pressure concentration to obtain respectively.
The present invention relates to the preparation method of above-mentioned locust tree plant acaricide, be specially: respectively locust tree branch, stem, leaf, flower are clayed into power after drying, adopt again the method for reduced pressure concentration to obtain respectively medicinal extract using ethanol as extraction solvent, finally medicinal extract is scattered in distilled water and makes miticide by above-mentioned formula.
Described oven dry refers to: at 30-50 DEG C, dry.
Described powder refers to: 20-40 object particulate powder.
Described ethanol refers to: the ethanolic solution of 25%-70%v/v or absolute ethyl alcohol, the 10-20 that its consumption is powder quality doubly.
Described reduced pressure concentration refers to: the crude extract obtaining after lixiviate is vacuumized to decompression to-0.1 atmospheric pressure and is placed in water-bath at 40-80 DEG C, make crude extract simmer down to medicinal extract.
Described dispersion refers to: adopt the mode of ultrasonic dispersion that medicinal extract is scattered in distilled water.
The present invention relates to the application of above-mentioned locust tree plant acaricide, under the stirring environment of 30-50 DEG C, water is added wherein and be diluted to the concentration of 1-3wt% after be cooled to normal temperature, form aqua.
The present invention has the following advantages compared with the prior art:
(1) between the primary raw material of composition product of the present invention, synergy on the one hand can strengthen miticidal effect, on the other hand due to not
The pesticide resistance that the acting in conjunction of the active ingredient of same-action mechanism can delay medicament produces.
(2) raw material be easy to get, process simple, low for equipment requirements, reclaim after solvent also can reuse.
(3) this miticide derives from natural traditional Chinese medicine plant extracts, and utilizes its water-soluble feature, is made into aqua, so its environmentally safe, to people, animal safety, is the biological miticide of a kind of pollution-free plant source.
Embodiment
Below embodiments of the invention are elaborated, the present embodiment is implemented as prerequisite taking technical solution of the present invention, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
Control Tetranychus cinnabarinus aqua percentage by weight: locust tree branch extract 10%, locust tree stem extraction 10%, leaf of locust extract 5%, Pagoda Flower extract 15% and distilled water 60%.
To after locust tree branch, stem, leaf, 30-50 DEG C of oven dry of flower, clay into power, take respectively the each 20g in locust tree branch, stem, leaf and pollen end (20-40 order particle), ethanolic solution 800ml with 70% is at room temperature dipping 1-3 days, filter, the crude extract obtaining after lixiviate vacuumized to decompression to-0.1 atmospheric pressure and is placed in water-bath at 40-80 DEG C, make crude extract simmer down to medicinal extract, adding distilled water, be placed in supersonic generator 10-15min dry matter is suspended, be mixed with locust tree plant acaricide.Under the stirring environment of 30-50 DEG C, water is added wherein and be diluted to the concentration of 1wt% after be cooled to normal temperature, form aqua.
Test method:
This locust tree plant acaricide soaks stem infusion process to the determination of activity of Tetranychus cinnabarinus according to the raw tetranychid of surveying in SOP in the southern agricultural chemicals initiative of country center to carry out.Process by embodiment 1 the plant resource miticide obtaining, set the not blank of adding medicine simultaneously.
Become mite to be inoculated on ready blade the tetranychid of indoor feeding, make to remain on 30-60 head for the one-tenth mite number on examination blade.After the off-the-shelf broad bean seedling with mite is soaked to liquid 5s, take out, finally again the broad bean seedling having soaked is inserted and is equipped with in the penicillin bottle of clear water, every processing repeats for 3 times.Examination worm after treatment is placed under normal condition and is cultivated, and examination worm response situation is observed in timing, 24 hours each life or death borer populations of processing of " Invest, Then Investigate ", and its preventive effect is 85.53%.
Embodiment 2
Control Tetranychus cinnabarinus aqua percentage by weight: locust tree branch extract 40%, locust tree stem extraction 10%, leaf of locust extract 15%, Pagoda Flower extract 5% and distilled water 30%.
Take respectively locust tree branch, stem, leaf, the each 50g in pollen end (20-40 order particle), at room temperature flood 1-3 days with 50% ethanolic solution 2000ml, filter, the crude extract obtaining after lixiviate is vacuumized to decompression to-0.1 atmospheric pressure and is placed in water-bath at 40 DEG C, make crude extract simmer down to medicinal extract, add distilled water, be placed in supersonic generator 15min dry matter is suspended, be mixed with locust tree plant acaricide.Under the stirring environment of 30-50 DEG C, water is added wherein and be diluted to the concentration of 1wt% after be cooled to normal temperature, form aqua.Adopt tetranychid to soak stem leaf dipping method to Tetranychus cinnabarinus and give birth to survey, after 24 hours, add up lethality, its preventive effect is 88.34%.
Embodiment 3
Control Tetranychus cinnabarinus aqua percentage by weight: locust tree branch extract 15%, locust tree stem extraction 15%, leaf of locust extract 35%, Pagoda Flower extract 5% and distilled water 20%.
Take respectively locust tree branch, stem, leaf, the each 50g in pollen end (20-40 order particle), at room temperature flood 1-3 days with 25% ethanolic solution 2000ml, filter, the crude extract obtaining after lixiviate is vacuumized to decompression to-0.1 atmospheric pressure and is placed in water-bath at 80 DEG C, make crude extract simmer down to medicinal extract, add distilled water, be placed in supersonic generator 15min dry matter is suspended, be mixed with locust tree plant acaricide.Under the stirring environment of 30-50 DEG C, water is added wherein and be diluted to the concentration of 3wt% after be cooled to normal temperature, form aqua.Adopt tetranychid to soak stem leaf dipping method to Tetranychus cinnabarinus and give birth to survey, after 24 hours, add up lethality, its preventive effect is 92.54%.
Embodiment 4
Control Tetranychus cinnabarinus aqua percentage by weight: locust tree branch extract 10%, locust tree stem extraction 20%, leaf of locust extract 10%, Pagoda Flower extract 35% and distilled water 25%.
Take respectively locust tree branch, stem, leaf, the each 50g in pollen end (20-40 order particle), at room temperature flood 1-3 days with ethanol solution 2000ml, filter, the crude extract obtaining after lixiviate is vacuumized to decompression to-0.1 atmospheric pressure and is placed in water-bath at 50 DEG C, make crude extract simmer down to medicinal extract, add distilled water, be placed in supersonic generator 15min dry matter is suspended, be mixed with locust tree plant acaricide.Under the stirring environment of 30-50 DEG C, water is added wherein and be diluted to the concentration of 2wt% after be cooled to normal temperature, form aqua.Adopt indoor pot method to give birth to survey to Tetranychus cinnabarinus, after 24 hours, add up lethality, its preventive effect is 88.89%.
Embodiment 5
Control Tetranychus cinnabarinus aqua percentage by weight: locust tree branch extract 15%, locust tree stem extraction 40%, leaf of locust extract 20%, Pagoda Flower extract 5% and distilled water 20%.
Take respectively locust tree branch, stem, leaf, the each 50g in pollen end (20-40 order particle), at room temperature flood 1-3 days with 70% ethanolic solution 2000ml, filter, the crude extract obtaining after lixiviate is vacuumized to decompression to-0.1 atmospheric pressure and is placed in water-bath at 60 DEG C, make crude extract simmer down to medicinal extract, add distilled water, be placed in supersonic generator 15min dry matter is suspended, be mixed with locust tree plant acaricide.Under the stirring environment of 30-50 DEG C, water is added wherein and be diluted to the concentration of 1wt% after be cooled to normal temperature, form aqua.Adopt the experiment of community, field Tetranychus cinnabarinus is carried out to field control effectiveness test, after 24 hours, add up lethality, preventive effect reaches 84.56%.
Although content of the present invention has been done detailed introduction by above preferred embodiment, will be appreciated that above-mentioned description should not be considered to limitation of the present invention.Read after foregoing those skilled in the art, for multiple amendment of the present invention and substitute will be all apparent.Therefore, protection scope of the present invention should be limited to the appended claims.
Claims (4)
1. a locust tree plant acaricide, is characterized in that being made up of following component and weight percent content: locust tree branch extract 10～40%, locust tree stem extraction 10～40%, leaf of locust extract 5～35%, Pagoda Flower extract 5～35% and distilled water 20～60%; After drying at 30-50 DEG C, locust tree branch, stem, leaf, flower, wear into 20-40 object powder respectively, then adopt the method for reduced pressure concentration to obtain respectively medicinal extract using ethanol as extraction solvent, finally medicinal extract is scattered in distilled water and makes miticide by above-mentioned formula; Described reduced pressure concentration refers to: the crude extract obtaining after lixiviate is vacuumized to decompression to-0.1 atmospheric pressure and is placed in water-bath at 40-80 DEG C, make crude extract simmer down to medicinal extract.
2. locust tree plant acaricide according to claim 1, is characterized in that: described ethanol refers to: the ethanolic solution of 25%-70%v/v or absolute ethyl alcohol, the 10-20 that its consumption is powder quality doubly.
3. locust tree plant acaricide according to claim 1 and 2, is characterized in that: described dispersion refers to: adopt the mode of ultrasonic dispersion that medicinal extract is scattered in distilled water.
4. an application for locust tree plant acaricide as claimed in claim 1, is characterized in that: described locust tree plant acaricide under the stirring environment of 30-50 DEG C, water is added wherein and be diluted to the concentration of 1-3wt% after be cooled to normal temperature, form aqua and spray and use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210137309.6A CN102687733B (en) | 2012-05-03 | 2012-05-03 | Black locust plant acaricide, preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210137309.6A CN102687733B (en) | 2012-05-03 | 2012-05-03 | Black locust plant acaricide, preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102687733A CN102687733A (en) | 2012-09-26 |
| CN102687733B true CN102687733B (en) | 2014-06-18 |
Family
ID=46853619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210137309.6A Expired - Fee Related CN102687733B (en) | 2012-05-03 | 2012-05-03 | Black locust plant acaricide, preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102687733B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2607147B1 (en) * | 2015-09-29 | 2018-01-17 | Biopharma Research, S.A. | USE OF AN ACACIA VEGETABLE EXTRACT TO PROMOTE RADICULAR GROWTH AND ACTIVITY IN PLANTS |
| CN105638755B (en) * | 2016-03-23 | 2018-06-19 | 国际竹藤中心 | A kind of plant insecticide and preparation method thereof |
| CN105746610B (en) * | 2016-03-23 | 2018-06-26 | 国际竹藤中心 | A kind of synergist of plant source aphicide |
| CN105901022A (en) * | 2016-04-29 | 2016-08-31 | 山东胜伟园林科技有限公司 | Muscardine insecticide suitable for diseases and pests of Chinese cabbage and preparation method of muscardine insecticide |
| CN105831168A (en) * | 2016-04-29 | 2016-08-10 | 山东胜伟园林科技有限公司 | Botanical pesticide and preparing method thereof |
| CN105925272A (en) * | 2016-05-31 | 2016-09-07 | 山东胜伟园林科技有限公司 | Method for greening original soil of alkaline sandy soil with soil conditioner containing peanut vine powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101356930A (en) * | 2007-07-31 | 2009-02-04 | 北京农学院 | Brucea fruit, honeylocust fruit or amorpha phytogenic miticide and preparation method thereof |
| CN101595895A (en) * | 2007-07-31 | 2009-12-09 | 北京农学院 | Amorpha fruticosa plant miticide and preparation method thereof |
-
2012
- 2012-05-03 CN CN201210137309.6A patent/CN102687733B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101356930A (en) * | 2007-07-31 | 2009-02-04 | 北京农学院 | Brucea fruit, honeylocust fruit or amorpha phytogenic miticide and preparation method thereof |
| CN101595895A (en) * | 2007-07-31 | 2009-12-09 | 北京农学院 | Amorpha fruticosa plant miticide and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 8种植物提取物对朱砂叶螨生物活性筛选及其研究;贾福丽等;《中国农学通报》;20111231(第24期);第287页第1.2部分，以及表1、2 * |
| 贾福丽等.8种植物提取物对朱砂叶螨生物活性筛选及其研究.《中国农学通报》.2011,(第24期), |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102687733A (en) | 2012-09-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102687733B (en) | Black locust plant acaricide, preparation method and application thereof | |
| CN102972452B (en) | Biological plant source miticide prepared from urban greening wastes and preparation and application of biological plant source miticide | |
| CN102038014B (en) | Compound plant-source acaricide and preparation method thereof | |
| CN104430604A (en) | Oxymatrine emulsifiable concentrate pesticide | |
| CN101999418A (en) | Plant source pesticide for preventing and controlling golden autumn pear diseases and insect pests and preparation and application thereof | |
| CN103329943A (en) | Insecticide composition containing natural celastrus angulatus and natural pyrethrin | |
| CN104996500A (en) | Compound plant pesticide capable of controlling plant aphid, and preparation and application thereof | |
| CN101796968B (en) | Method for preparing preparation for preventing plant aphids by utilizing tomato stem and leaf extract | |
| CN103141488A (en) | Pesticide compounded by azadirachtin and cnidium lactone and preparation method thereof | |
| CN103609597A (en) | Botanical synergistic compound pesticide | |
| CN102204575B (en) | Silktree-albizia-extract-based preparation for controlling tetranychus cinnabarinus, and preparation and application thereof | |
| CN110169422B (en) | Compound formulation plant bactericide and preparation method and application thereof | |
| CN110150330A (en) | A kind of compound botanical source bactericide and preparation method thereof | |
| CN108719345B (en) | Origanum vulgaris essential oil preparation, preparation method and application thereof | |
| CN103493831A (en) | Insecticide composition with insecticides of pyriminostrobin and tetronic acid | |
| CN102224831B (en) | Control method of tetranychus cinnabarinus based on tomato extracts | |
| CN103907648A (en) | Preparation and application of compound plant bactericide for preventing and treating plant downy mildew | |
| CN101336647A (en) | Plant composite insecticide | |
| CN103548884B (en) | Chinese pistache kills Tetranychus cinnabarinus agent and preparation method thereof | |
| CN106490048A (en) | A kind of environment friendly agricultural of preventing and treating brassicaceous vegetable pest and disease damage and preparation method thereof | |
| CN102687734B (en) | Plant-based acaricide for preventing and controlling tetranychus cinnabarinus, preparation method and application thereof | |
| CN102487966A (en) | Botanical pesticide for effectively controlling Dioryctria splendidella Herrich-Schaeffer, preparation thereof and application thereof | |
| CN105494456A (en) | Pollution-free sterilizing agent capable of preventing and treating trunk blight disease of waxberries and preparation method thereof | |
| Rana et al. | Screening of Calophyllum inophyllum L. leaf extracts for cytotoxic, larvicidal, insect repellent and antimicrobial activities | |
| CN102687732A (en) | Plant acaricide of Japanese creeper and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140618 Termination date: 20170503 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |