CN103329943A - Insecticide composition containing natural celastrus angulatus and natural pyrethrin - Google Patents
Insecticide composition containing natural celastrus angulatus and natural pyrethrin Download PDFInfo
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Abstract
The invention relates to an insecticide composition containing natural celastrus angulatus and natural pyrethrin. The Insecticide composition containing the natural celastrus angulatus and the natural pyrethrin consists of the natural celastrus angulatus and the natural pyrethrin in the weight ratio of (1-20) to (1-20), and is used for preparing a medicament for preventing and curing aphididae pests of farmland wheat aphids, lipaphis erysimi, aphis citricola and the like. The insecticide composition containing the natural celastrus angulatus and the natural pyrethrin, provided by the invention, has a good insecticidal effect, is low in pharmacy cost, has no residue and pollution, is safe to human beings and livestock, and can not generate drug resistance, and by utilizing the complementary advantages of two kinds of botanical pesticides, the defects of poor fast-acting property, slow pesticide effect, low knocking-off speed rate of the natural celastrus angulatus, as well as poor natural pyrethrin stability, high production and pharmacy cost and the like of the natural pyrethrin can be fully improved.
Description
Technical field
The present invention relates to pesticide field, be specifically related to a kind of Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum.
Background technology
In recent years, high poison, high residue chemical pesticide become the major issue of pollution of ecological environment, harm food security and common people's health.Along with the understanding of people to chemical pesticide harm, world developed country and part developing country have all limited the use of poisonous chemical pesticide, and many countries have formulated and reduced the countermeasure that chemical pesticide uses, and propose the substitute products of chemical pesticide.China is a large agricultural country, and agricultural chemicals is being brought into play very important effect in the agricultural production of China.Chemical pesticide is used in strict restriction, solves residue of pesticide and exceeds standard, and the safety and the agricultural products in China that improve agricultural product are urgent in the competitiveness demand of international market.
For adapting to China's agricultural sustainable development strategy; the developing direction of agricultural chemicals will be to turn to selective pesticide from non-selective agricultural chemicals; turn to " ecological agricultural chemicals rationally " and " the harmonious agricultural chemicals of environment " from traditional organic chemicals, be beneficial to protect environment, promote the sustainable development of agricultural.Therefore, many biopesticides are strong owing to having selectivity, and are less to the influence of non-target organism to the person poultry safety, can keep balance of nature; In natural environment, be easy to degraded, noresidue, free from environmental pollution; Multiple factor and composition play a role, pest advantage and come into one's own day by day that (Xu Hanhong, Zhang Zhi is auspicious, Cheng Dongmei such as be difficult to develop immunity to drugs.The advantages for development of biopesticide and existing problems.World's agricultural chemicals, 2004,26 (2): 5-10).
Botanical pesticide is one of biopesticide the most ancient on the human history, it derives from nature, in natural environment easily degraded, safety nuisance free, now become one of green bio agricultural chemicals first-selection.Natural plant product Celastrus angulatus derive from traditional pesticide plant Celastraceae Celastrus Celastrus angulatus (Celastrus angulatus Max.) (Wu Wenjun. pesticide plant Celastrus angulatus research. agricultural chemicals .1991,30).ABUNDANT NATUREAL RESOURSES extensively is distributed in the Yellow River, the Yangtze river basin, and is the most concentrated with Henan, Shaanxi, Hubei; Its well developed root system, environment-adapting ability and regeneration capacity are all very strong, and ripe (Lu Lingxian, Nan Yusheng, Ke Zhiguo, ten thousand Gan, Xu Huizhu, Wu Dongyun of its artificial breeding technology at present.The pesticide plant Celastrus angulatus is introduced a fine variety breeding research preliminary study.The Hubei forestry science and technology, 1987,61 (3): 8-10).
The applicant has actively developed botanical pesticide-natural Celastrus angulatus LS20030503 in conjunction with the great demand of international advanced technology and domestic and international market.The natural Celastrus angulatus of botanical pesticide has certain control efficiency to aphid section insects such as wheat aphid, radish aphids, but its single agent quick-acting is relatively poor, exists the insect drug effect slowly, and field efficacy is general, the spray medicine often, defective such as extension rate is lower.Dalmatian chrysanthemum (Pyrethrum cinerariifolium) is a kind of perennial herb feverfew, and the natural pyrethrum in its capitulum has the effect of paralysis insect nervous centralis, is the killing insects by touching agent.The existing many reports of pest such as natural pyrethrum is prevented eliminating aphis, mite class, and to crop safety nonhazardous (Zhang Xiating, Nie Qiulin, Gao Xin.The insecticidal properties of natural pyrethrum and the mechanism of action.Pesticide science and management, 2003,24 (2): 22-23).But natural pyrethrum in high light and air easily oxidized, decompose, and the production cost height (Cheng Xuan gives birth to, and Zhao Ping is in gushing.Pyrethrum.Agricultural chemicals, 2005,44 (9): 391-394).
Summary of the invention
The purpose of this invention is to provide that a kind of good disinsection effect, drug cost are low, noresidue, pollution-free, to person poultry safety, the Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum that is difficult for developing immunity to drugs, utilize two kinds of plant source pesticides to have complementary advantages, it is poor to substantially improve natural Celastrus angulatus quick-acting, drug effect is slow, knock down the on the low side and natural pyrethrum poor stability of speed, defective such as produce and drug cost is higher.
Another purpose of the present invention is to provide a kind of described preparation method who contains the complex insecticidal composition of natural Celastrus angulatus.
To achieve these goals, technical scheme of the present invention is such:
A kind of Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum is characterized in that: be (1～20) by weight ratio: natural Celastrus angulatus and the natural pyrethrum of (1～20) are formed.
A kind of Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum is characterized in that: be (1～10) by weight ratio: natural Celastrus angulatus and the natural pyrethrum of (1～10) are formed.
Described natural Celastrus angulatus preparation method is: root bark of celastrus angulatus is pulverized, get the root bark of celastrus angulatus powder, first organic solvent that adds 3～5 times of root bark of celastrus angulatus grain weight amounts, extract 1～4h at 60～80 ℃, described first organic solvent is a kind of in methyl alcohol, benzinum, benzene, 95% the ethanol.
Described natural pyrethrum preparation method is: pyrethrum flowers is pulverized, get pulvis flores pyrethri, second organic solvent that adds 3～5 times of pulvis flores pyrethri weight, extract 1～6h at 30～50 ℃, be evaporated to the paste crude extract, the mixed liquor that adds the first alcohol and water of 5 times of paste crude extract weight then, stir, when being placed to the upper strata and being yellow clarified solution, remove upper strata liquid, be evaporated to paste, the mixed liquor of described first alcohol and water is mixed by the first alcohol and water of weight ratio 1:1 to be formed, and described second organic solvent is benzene, ethyl acetate, a kind of in the benzinum.
The Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum is for the preparation of the medicine of the anti-section's agricultural pests that eliminates aphis.
Described agricultural pests is wheat aphid, radish aphid, apple yellow aphid aphid section insect.
The aqueous emulsion of a kind of natural Celastrus angulatus and natural pyrethrum, by weight percent meter: the Pesticidal combination 0.1～15%, solvent cyclohexanone 3～36%, preservative sodium benzoate 0.05～1%, antifreeze glycol 0.5～1.5%, synergist Butacide 1～5%, stabilizing agent 1,2-butanediol 0.5～2%, the emulsifier farming breast 2201 that contain natural Celastrus angulatus and natural pyrethrum be 0.2～5% and surplus be that water is formed.
The aqueous emulsion of a kind of natural Celastrus angulatus and natural pyrethrum, by weight percent meter: natural Celastrus angulatus is 2%, natural pyrethrum is 0.2%, the solvent cyclohexanone is 7%, preservative sodium benzoate is 0.08%, antifreeze glycol is 0.7%, synergist Butacide 1.5%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 1.5% and surplus be water is formed.
The aqueous emulsion of a kind of natural Celastrus angulatus and natural pyrethrum, by weight percent meter: natural Celastrus angulatus is 2.4%, natural pyrethrum is 0.6%, the solvent cyclohexanone is 9%, preservative sodium benzoate is 0.2%, antifreeze glycol is 1%, synergist Butacide 2%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 2% and surplus be water is formed.
The aqueous emulsion of a kind of natural Celastrus angulatus and natural pyrethrum, by weight percent meter: natural Celastrus angulatus is 0.5%, natural pyrethrum is 0.5%, the solvent cyclohexanone is 3%, preservative sodium benzoate is 0.06%, antifreeze glycol is 0.5%, synergist Butacide 3%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 2% and surplus be water is formed.
The invention has the advantages that: (1) preparation with water is matrix, and is environmentally safe, nonflammable, and storing is convenient; (2) compare with single agent, the generation of pest resistance to insecticide is grown, delayed to drug effect height, lasting period; (3) antagonism aphid, leafhopper have good control efficiency; (4) active ingredient can be degraded in nature, and is good with Environmental compatibility, noresidue, pollution-free, and safety, environmental protection are to characteristics such as person poultry safeties.
Embodiment:
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 1:3.15%
By weight percent meter: natural Celastrus angulatus 3%, natural pyrethrum 0.15%, solvent cyclohexanone 9%, preservative sodium benzoate 0.1%, antifreeze glycol 0.7%, synergist Butacide 1.6%, stabilizing agent 1,2-butanediol 0.6%, emulsifier farming breast 2201 is 1.6% and the water of surplus, mixes composition.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 2:2.2%
By weight percent meter: natural Celastrus angulatus is 2%, natural pyrethrum is 0.2%, the solvent cyclohexanone is 7%, preservative sodium benzoate is 0.08%, antifreeze glycol is 0.7%, synergist Butacide 1.5%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 1.5% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 3:3%
By weight percent meter: natural Celastrus angulatus is 2.4%, natural pyrethrum is 0.6%, the solvent cyclohexanone is 9%, preservative sodium benzoate is 0.2%, antifreeze glycol is 1%, synergist Butacide 2%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 2% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 4:4%
By weight percent meter: natural Celastrus angulatus 3%, natural pyrethrum 1%, solvent cyclohexanone 9%, preservative sodium benzoate 0.5%, antifreeze glycol are 0.8%, synergist Butacide 1.5%, stabilizing agent 1,2-butanediol is 0.7%, farming breast 2201 is 4% and the water of surplus, mixes composition.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 5:1%
By weight percent meter: natural Celastrus angulatus is 0.5%, natural pyrethrum is 0.5%, the solvent cyclohexanone is 3%, preservative sodium benzoate is 0.06%, antifreeze glycol is 0.5%, synergist Butacide 3%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 2% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 6:5%
By weight percent meter: natural Celastrus angulatus 1%, natural pyrethrum 4%, cyclohexanone 20%, Sodium Benzoate 0.5%, ethylene glycol 1%, Butacide 1%, 1,2-butanediol 1%, farming breast 2201 be 3% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 7:3.5%
By weight percent meter: natural Celastrus angulatus is 0.5%, natural pyrethrum is 3%, the solvent cyclohexanone is 9%, preservative sodium benzoate is 0.08%, antifreeze glycol is 0.8%, synergist Butacide 1.5%, stabilizing agent 1,2-butanediol are 0.7%, emulsifier farming breast 2201 be 2% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 8:5.5%
By weight percent meter: natural Celastrus angulatus is 0.5%, natural pyrethrum is 5%, the solvent cyclohexanone is 25%, preservative sodium benzoate is 0.8%, antifreeze glycol is 1%, synergist Butacide 5%, stabilizing agent 1,2-butanediol are 1.6%, emulsifier farming breast 2201 be 4.5% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 9:6.5%
By weight percent meter: natural Celastrus angulatus is 0.5%, natural pyrethrum is 6%, the solvent cyclohexanone is 15%, preservative sodium benzoate is 0.8%, antifreeze glycol is 1%, synergist Butacide 5%, stabilizing agent 1,2-butanediol are 1.6%, emulsifier farming breast 2201 be 4.5% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 10:10.5%
By weight percent meter: natural Celastrus angulatus is 0.5%, natural pyrethrum is 10%, the solvent cyclohexanone is 35%, preservative sodium benzoate is 0.9%, antifreeze glycol is 1.2%, synergist Butacide 4.5%, stabilizing agent 1,2-butanediol are 1.8%, emulsifier farming breast 2201 be 4.5% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 11:0.1%
By weight percent meter: natural Celastrus angulatus is 0.05%, natural pyrethrum is 0.05%, the solvent cyclohexanone is 3%, preservative sodium benzoate is 0.05%, antifreeze glycol is 0.5%, synergist Butacide 1%, stabilizing agent 1,2-butanediol are 0.5%, emulsifier farming breast 2201 be 0.2% and surplus be water is formed.
The preparation of the natural Celastrus angulatus pyrethrin water emulsion of embodiment 12:15%
By weight percent meter: natural Celastrus angulatus is 7%, natural pyrethrum is 8%, the solvent cyclohexanone is 36%, preservative sodium benzoate is 1%, antifreeze glycol is 1.5%, synergist Butacide 5%, stabilizing agent 1,2-butanediol are 2%, emulsifier farming breast 2201 be 5% and surplus be water is formed.
The present invention be directed to the deficiency of natural Celastrus angulatus and natural pyrethrum, introducing traditional Chinese medicine theory, natural Celastrus angulatus and natural pyrethrum are carried out active component assembly 5, to reach the purpose that two kinds of plant source pesticides are had complementary advantages, it is poor to substantially improve natural Celastrus angulatus quick-acting, defectives such as drug effect is slow, knocks down the on the low side and natural pyrethrum poor stability of speed, and production and drug cost are higher.To so far, do not see the composite report of natural Celastrus angulatus and natural pyrethrum.The present invention and through repetition test has repeatedly obtained good effect, and is specific as follows:
To the rhopalosiphum padi Toxicity Determination:
Take by weighing natural Celastrus angulatus and the natural pyrethrum of 10mg respectively, farming breast 2201 with 1g cyclohexanone and mass concentration 2% is made into the 10000ppm mother liquor, natural Celastrus angulatus and two kinds of mother liquors of natural pyrethrum are made into the binary built agent as 7 processing by 20:1,10:1,4:1,1:1,1:4,1:10,1:20 mixing respectively, 5 concentration of dilute with water are made blank with the clear water processing respectively; Worm is soaked in employing, leaf dipping method carries out indoor synergistic effect biologicall test.Aphid immersed in the soup together with wheat seeding takes out after 5 seconds, with blotting paper inhale go unnecessary soup or air-dry after, reject alatae, only keep wingless aphid, put it in the culture dish, with the filter paper raising of preserving moisture, put into 25 ℃ of illumination boxs then, investigate the survival borer population after 48 hours respectively.The gained lethality is proofreaied and correct with the Abbott formula, according to the return law of the straight line of drug concentration logarithm value and corrected mortality probit value, calculates virulence regression equation, the lethal concentration of 50 (LC
50).Mixture adopts the abundant grade of Sun Yun (1960) formula to obtain co-toxicity coefficient (CTC) (Sun Y P, E R Johnnson.Analysis of joint action of pesticides against house files[J] .Ecological Entomology, 1960,53:887-891).The synergy criterion: co-toxicity coefficient (CTC) is remarkable synergy greater than 200, and 150～200 is less for synergistic effect, and 70～150 is summation action, is antagonism (Tan Fujie, Shen Jinliang, You Ziping less than 70.Insecticide mixes the research [J] of using.Agricultural University Of Nanjing's newspaper, 1987,4 (increasing): 93-99).
Single agent toxicity index=(standard insecticide LC
50/ for surveying insecticide LC
50) * 100
The toxicity index of theoretical (A+B) intermixture of toxicity index/confession of CTC=[actual measurement (A+B) intermixture] * 100 actual measurements
(A+B) toxicity index of intermixture=standard insecticide LC
50/ (A+B) intermixture LC
50* 100
Toxicity index=A * the A of theoretical (A+B) intermixture
C+ B * B
C
In the formula: A represents the single agent toxicity index of A; AC represents A shared percentage in mixture; B represents the single agent toxicity index of B;
BC represents B shared percentage in mixture.Supplying the examination insect is rhopalosiphum padi.Measure result of calculation and see Table 1:
The natural Celastrus angulatus of table 1 and natural pyrethrum are to 48 hours virulence of rhopalosiphum padi malicious coefficient determination result together
Measurement result according to table 1 shows: two kinds of active ingredient groups of natural Celastrus angulatus and natural pyrethrum have significant synergistic effect in the composite back of the ratio in the table 1 to rhopalosiphum padi, when natural Celastrus angulatus and natural pyrethrum 4:1, synergistic effect is the strongest, and co-toxicity coefficient is 257.276; When ratio was 20:1,10:1,1:1,1:4,1:10,1:20, co-toxicity coefficient was followed successively by 185.733,240.377,213.929,200.532,189.704,154.536.
To the radish aphid Toxicity Determination:
Take by weighing natural Celastrus angulatus and the natural pyrethrum of 10mg respectively, be that 2% farming breast 2201 is made into the 10000ppm mother liquor with 1g cyclohexanone and mass concentration, natural Celastrus angulatus and two kinds of mother liquors of natural pyrethrum are made into the binary built agent as 7 processing by 20:1,10:1,4:1,1:1,1:4,1:10,1:20 mixing respectively, 5 concentration of dilute with water are made blank with the clear water processing respectively; Worm is soaked in employing, leaf dipping method carries out indoor synergistic effect biologicall test.Aphid is immersed taking-up after 5 seconds in the soup together with cabbage leaves, with blotting paper inhale go unnecessary soup or air-dry after, reject alatae, only keep wingless aphid, put it in the culture dish, with the filter paper raising of preserving moisture, put into 25 ℃ of illumination boxs then, investigate the survival borer population after 48 hours respectively, calculate virulence regression equation, the lethal concentration of 50 (LC
50) and co-toxicity coefficient.Supplying the examination insect is radish aphid.Measure result of calculation and see Table 2.
The natural Celastrus angulatus of table 2 and natural pyrethrum are to 48 hours virulence of radish aphid malicious coefficient determination result together
Two kinds of active ingredient groups of natural Celastrus angulatus and natural pyrethrum are composite back to radish aphid by a certain percentage, significant synergistic effect is arranged when 10:1～1:10, when natural Celastrus angulatus and natural pyrethrum 4:1, synergistic effect is the strongest, and co-toxicity coefficient is 261.168; Ratio is 20:1,10:1,1:1,1:4,1:10,1:20, and co-toxicity coefficient is followed successively by 178.493,223.030,233.918,214.003,204.999,166.723.
To the apple yellow aphid Toxicity Determination:
Take by weighing natural Celastrus angulatus and the natural pyrethrum of 10mg respectively, be that 2% farming breast 2201 is made into the 10000ppm mother liquor with 1g cyclohexanone and mass concentration, natural Celastrus angulatus and two kinds of mother liquors of natural pyrethrum are made into the binary built agent as 7 processing by 20:1,10:1,4:1,1:1,1:4,1:10,1:20 mixing respectively, 5 concentration of dilute with water are made blank with the clear water processing respectively; Worm is soaked in employing, leaf dipping method carries out indoor synergistic effect biologicall test.Aphid is immersed taking-up after 5 seconds in the soup together with the apple blade, with blotting paper inhale go unnecessary soup or air-dry after, reject alatae, only keep wingless aphid, put it in the culture dish, with the filter paper raising of preserving moisture, put into 25 ℃ of illumination boxs then, investigate the survival borer population after 48 hours respectively, calculate virulence regression equation, the lethal concentration of 50 (LC
50) and co-toxicity coefficient.Supplying the examination insect is apple yellow aphid.Measure result of calculation and see Table 3.
The natural Celastrus angulatus of table 3 and natural pyrethrum are to 48 hours virulence of apple yellow aphid malicious coefficient determination result together
After two kinds of active ingredient groups of natural Celastrus angulatus and natural pyrethrum are composite in the ratio in the table 3, apple yellow aphid is had significant synergistic effect, when natural Celastrus angulatus and natural pyrethrum 4:1, synergistic effect is the strongest, and co-toxicity coefficient is 246.824; Ratio is 20:1,10:1,1:1,1:4,1:10,1:20, and co-toxicity coefficient is followed successively by 172.426,211.021,205.879,200.974,184.173,136.655.
Control wheat aphid field control effectiveness test:
Crop: wheat; Controlling object: aphid
5 processing are established in test, repeat 4 times, amount to 20 experimental plots.District's group is arranged at random, residential quarter area 20m
2Every mu of 50 kg of water, the spraying of bodyguard's board WS-16P knapsack hand sprayer is handled.Before dispenser, in every residential quarter, press 5 samplings of diagonal, list and fix 5 points, choose wheat 6 strains that aphid is arranged at every, whenever repeat altogether 30 strains, aphid number on statistics boot leaf and the wheat head, with this as dispenser before the insect population radix.In the 1st, 3 and 7 day investigation aphid number of deciding to survive on strain boot leaf and the wheat head respectively after the dispenser.
Go down rate as control efficiency to proofread and correct insect population, and computing formula is as follows:
The embodiment 5 preparations control wheat aphid field control effectiveness test of this patent, control efficiency sees Table 4.
Table 4 embodiment 5 preparations control wheat aphid field control effectiveness test
The embodiment 5 preparations control wheat aphid field control effectiveness test of this patent, control efficiency sees Table 5.
Table 5 embodiment 3 preparations control wheat aphid field control effectiveness test
Two kinds of composite preparations of active ingredient group of the present invention significantly improve the wheat aphid control efficiency, and quick-acting also is significantly improved.When every mu of consumption was 100g, 1% natural Celastrus angulatus pyrethrin preventive effect was about 80%; During 3% natural Celastrus angulatus pyrethrin 60～100g/ mu consumption, the control efficiency of wheat aphid all about 90%, obviously is better than two kinds of active ingredient groups and uses separately, and in this test scope of medication to target crop and beneficial organism safety.
Control brassicaceous vegetable (wild cabbage) radish aphid field control effectiveness test:
Crop: wild cabbage; Controlling object: radish aphid
5 processing are established in test, repeat 4 times, amount to 20 experimental plots.District's group is arranged at random, residential quarter area 20m
2Before dispenser, in every residential quarter, press 5 samplings of diagonal, list and fix 5 points, choose 2 in the blade that aphid is arranged at every, carry out insect population radix investigation records before the dispenser.Every mu of 50 kg of water, the spraying of bodyguard's board WS-16P knapsack hand sprayer is handled.Taking ditty checks the mark not after dispenser and to carry out in the 1st, 3 and 7 day.
Go down rate as control efficiency to proofread and correct insect population, and computing formula is as follows:
Embodiment 2 preparations control brassicaceous vegetable (wild cabbage) the radish aphid field control effectiveness test of this patent sees Table 6.
Table 6 embodiment 2 preparations control brassicaceous vegetable (wild cabbage) radish aphid field control effectiveness test
Embodiment 3 preparations control brassicaceous vegetable (wild cabbage) the radish aphid field control effectiveness test of this patent sees Table 7.
Table 7 embodiment 3 preparations control brassicaceous vegetable (wild cabbage) radish aphid field control effectiveness test
Handle chemical control brassicaceous vegetable (wild cabbage) radish aphid drug effect such as table 6 and table 7, as can be known, the composite preparation of two kinds of active ingredient groups of the present invention obviously improves brassicaceous vegetable (wild cabbage) radish aphid control efficiency, and quick-acting is preferably arranged.2.2% and 3% natural Celastrus angulatus pyrethrin 60～100g/ mu consumption, preventive effect is all 70%～90%; The control efficiency of brassicaceous vegetable (wild cabbage) radish aphid is better than two kinds of active ingredient groups uses separately, and in this test scope of medication to target crop and beneficial organism safety.
Control apple yellow aphid field control effectiveness test:
Crop: apple; Controlling object: aphid
5 processing are established in test, and each is handled and repeats 4 times, every residential quarter 2 strains tree, and the minizone is every strain, randomized arrangement.Soup evenly is sprayed on apple tree complete stool leave dual sides, till being sprayed onto blade and just dripping, every mu of 100 kg of water.Before the spray medicine each residential quarter by East, West, South, North, in 5 orientation respectively investigate 6 blades that apple yellow aphid is arranged, 30 leaves of every strain investigation, apple yellow aphid radix on the statistics spray medicine front vane, and the mark of listing.The aphid number alive that checked on the index blade of listing in the 1st, 3,7 day behind the spray medicine calculates each treatment region control efficiency.
Go down rate as control efficiency to proofread and correct insect population, and computing formula is as follows:
The embodiment 3 preparations control apple yellow aphid field control effectiveness test of this patent sees Table 8
Table 8 embodiment 3 preparations control apple yellow aphid field control effectiveness test
The embodiment 10 preparations control apple yellow aphid field control effectiveness test of this patent sees Table 9
Table 9 embodiment 10 preparations control apple yellow aphid field control effectiveness test
The composite preparation of two kinds of active ingredient groups of the present invention is to apple yellow aphid better prevention effect and quick-acting preferably, 3% and 10.5% natural Celastrus angulatus pyrethrin 120～200g/ mu consumption, the preventive effect of apple yellow aphid also all is better than two kinds of active ingredient groups uses separately, and in this test scope of medication to target crop and beneficial organism safety.
We have carried out the acute toxicity experience of small white mouse to natural Celastrus angulatus and the most significant proportioning 4:1 of natural pyrethrum synergistic effect, and regression equation is Y=-7.141+1.862x, acute oral LD
50Value is for 6829.605mg/kg, in little malicious scope.
In sum, Pesticidal combination of the present invention is that A is natural Celastrus angulatus, B is natural pyrethrum, this compound scheme has aphid section pest controling effect enhancings such as wheat aphid, radish aphid and apple yellow aphids, quick-acting is good, lasting effect is long, the advantage that one medicine is controlled more, compare with existing unitary agent, except having significant insecticidal effect, can effectively reduce the dispenser cost, slow down the generation of resistance, reduce the preparation residual quantity, pollution-free to crop, safety good, meets the security requirement of pesticidal preparations.
Claims (10)
1. Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum is characterized in that: be (1～20) by weight ratio: natural Celastrus angulatus and the natural pyrethrum of (1～20) are formed.
2. the Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum according to claim 1 is characterized in that: be (1～10) by weight ratio: natural Celastrus angulatus and the natural pyrethrum of (1～10) are formed.
3. the Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum according to claim 1, it is characterized in that, described natural Celastrus angulatus preparation method is: root bark of celastrus angulatus is pulverized, get the root bark of celastrus angulatus powder, first organic solvent that adds 3～5 times of root bark of celastrus angulatus grain weight amounts, extract 1～4h at 60～80 ℃, described first organic solvent is a kind of in methyl alcohol, benzinum, benzene, 95% the ethanol.
4. the Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum according to claim 1, it is characterized in that, described natural pyrethrum preparation method is: pyrethrum flowers is pulverized, get pulvis flores pyrethri, second organic solvent that adds 3～5 times of pulvis flores pyrethri weight, extract 1～6h at 30～50 ℃, be evaporated to the paste crude extract, the mixed liquor that adds the first alcohol and water of 5 times of paste crude extract weight then, stir, when being placed to the upper strata and being yellow clarified solution, remove upper strata liquid, be evaporated to paste, the mixed liquor of described first alcohol and water is mixed by the first alcohol and water of weight ratio 1:1 to be formed, and described second organic solvent is benzene, ethyl acetate, a kind of in the benzinum.
5. the described Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum of claim 1 is characterized in that, for the preparation of the medicine of the anti-section's agricultural pests that eliminates aphis.
6. the Pesticidal combination that contains natural Celastrus angulatus and natural pyrethrum according to claim 5 is characterized in that, described agricultural pests is wheat aphid, radish aphid, apple yellow aphid aphid section insect.
7. the aqueous emulsion of a natural Celastrus angulatus and natural pyrethrum, it is characterized in that, by weight percent meter: the Pesticidal combination 0.1～15%, solvent cyclohexanone 3～36%, preservative sodium benzoate 0.05～1%, antifreeze glycol 0.5～1.5%, synergist Butacide 1～5%, stabilizing agent 1,2-butanediol 0.5～2%, the emulsifier farming breast 2201 that contain natural Celastrus angulatus and natural pyrethrum be 0.2～5% and surplus be that water is formed.
8. the aqueous emulsion of natural Celastrus angulatus according to claim 7 and natural pyrethrum, it is characterized in that, by weight percent meter: natural Celastrus angulatus is 2%, natural pyrethrum is 0.2%, the solvent cyclohexanone is 7%, preservative sodium benzoate is 0.08%, antifreeze glycol is 0.7%, synergist Butacide 1.5%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 1.5% and surplus be water is formed.
9. the aqueous emulsion of natural Celastrus angulatus according to claim 7 and natural pyrethrum, it is characterized in that, by weight percent meter: natural Celastrus angulatus is 2.4%, natural pyrethrum is 0.6%, the solvent cyclohexanone is 9%, preservative sodium benzoate is 0.2%, antifreeze glycol is 1%, synergist Butacide 2%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 2% and surplus be water is formed.
10. the aqueous emulsion of natural Celastrus angulatus according to claim 7 and natural pyrethrum, it is characterized in that, by weight percent meter: natural Celastrus angulatus is 0.5%, natural pyrethrum is 0.5%, the solvent cyclohexanone is 3%, preservative sodium benzoate is 0.06%, antifreeze glycol is 0.5%, synergist Butacide 3%, stabilizing agent 1,2-butanediol are 0.6%, emulsifier farming breast 2201 be 2% and surplus be water is formed.
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| CN104145952A (en) * | 2014-07-18 | 2014-11-19 | 蔡荣昌 | Environment-friendly fragrant water-based household insecticide |
| CN105794807A (en) * | 2014-12-30 | 2016-07-27 | 刘启卓 | Prevention and treatment method for Aphis citricola on apples |
| CN106417349A (en) * | 2016-09-12 | 2017-02-22 | 广西南宁乐蕊生物科技有限责任公司 | Insecticidal composition containing bifenthrin and celangulin |
| CN106490066A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of microbial environment protecting insecticide and preparation method thereof |
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| CN106689225A (en) * | 2016-12-09 | 2017-05-24 | 河南省科高植物天然产物开发工程技术有限公司 | Novel insecticidal composition and preparation method and application thereof |
| CN112125731A (en) * | 2020-09-10 | 2020-12-25 | 河南省科学院 | Plant-source multifunctional liquid pesticide fertilizer and preparation method thereof |
| CN112250650A (en) * | 2020-10-10 | 2021-01-22 | 常州大学 | Celangulin original drug, emulsion in water and preparation method thereof |
| CN116076526A (en) * | 2022-12-29 | 2023-05-09 | 新沂市永诚化工有限公司 | Insecticidal composition containing celastrus angulatus and tolfenpyrad |
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| CN104145952A (en) * | 2014-07-18 | 2014-11-19 | 蔡荣昌 | Environment-friendly fragrant water-based household insecticide |
| CN105794807A (en) * | 2014-12-30 | 2016-07-27 | 刘启卓 | Prevention and treatment method for Aphis citricola on apples |
| CN106417349A (en) * | 2016-09-12 | 2017-02-22 | 广西南宁乐蕊生物科技有限责任公司 | Insecticidal composition containing bifenthrin and celangulin |
| CN106490066A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of microbial environment protecting insecticide and preparation method thereof |
| CN106665665A (en) * | 2016-12-09 | 2017-05-17 | 河南省科高植物天然产物开发工程技术有限公司 | Pesticidal composition and preparation method and application thereof |
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| CN106665665B (en) * | 2016-12-09 | 2019-08-30 | 河南省科高植物天然产物开发工程技术有限公司 | A kind of Pesticidal combination and the preparation method and application thereof |
| CN106689225B (en) * | 2016-12-09 | 2019-08-30 | 河南省科高植物天然产物开发工程技术有限公司 | A kind of novel insecticidal composition and the preparation method and application thereof |
| CN112125731A (en) * | 2020-09-10 | 2020-12-25 | 河南省科学院 | Plant-source multifunctional liquid pesticide fertilizer and preparation method thereof |
| CN112250650A (en) * | 2020-10-10 | 2021-01-22 | 常州大学 | Celangulin original drug, emulsion in water and preparation method thereof |
| CN116076526A (en) * | 2022-12-29 | 2023-05-09 | 新沂市永诚化工有限公司 | Insecticidal composition containing celastrus angulatus and tolfenpyrad |
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