CN106689225B - A kind of novel insecticidal composition and the preparation method and application thereof - Google Patents
A kind of novel insecticidal composition and the preparation method and application thereof Download PDFInfo
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- CN106689225B CN106689225B CN201611128516.XA CN201611128516A CN106689225B CN 106689225 B CN106689225 B CN 106689225B CN 201611128516 A CN201611128516 A CN 201611128516A CN 106689225 B CN106689225 B CN 106689225B
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- 229940116257 pepper extract Drugs 0.000 claims abstract description 45
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims abstract description 43
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- 239000000839 emulsion Substances 0.000 claims abstract description 24
- 239000000470 constituent Substances 0.000 claims abstract description 18
- FIPWRIJSWJWJAI-UHFFFAOYSA-N Butyl carbitol 6-propylpiperonyl ether Chemical compound C1=C(CCC)C(COCCOCCOCCCC)=CC2=C1OCO2 FIPWRIJSWJWJAI-UHFFFAOYSA-N 0.000 claims abstract description 17
- 210000000481 breast Anatomy 0.000 claims abstract description 17
- 229960005235 piperonyl butoxide Drugs 0.000 claims abstract description 17
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- 239000004299 sodium benzoate Substances 0.000 claims abstract description 17
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- 230000001502 supplementing effect Effects 0.000 claims abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- BMRWNKZVCUKKSR-UHFFFAOYSA-N butane-1,2-diol Chemical class CCC(O)CO BMRWNKZVCUKKSR-UHFFFAOYSA-N 0.000 claims description 12
- 238000002137 ultrasound extraction Methods 0.000 claims description 8
- 230000000361 pesticidal effect Effects 0.000 claims description 7
- 235000019441 ethanol Nutrition 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 6
- 241000949456 Zanthoxylum Species 0.000 claims description 5
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- 230000003111 delayed effect Effects 0.000 abstract description 2
- 239000011159 matrix material Substances 0.000 abstract description 2
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- 230000002745 absorbent Effects 0.000 description 1
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- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 235000017807 phytochemicals Nutrition 0.000 description 1
- 229930000223 plant secondary metabolite Natural products 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of novel insecticidal composition, active constituent are root bark of celastrus angulatus extract and pepper extract.Preparation method includes the following steps: 1) active constituent being mixed to obtain oil mixture with cyclohexanone；2) by agriculture breast 2201, sodium benzoate, ethylene glycol, Butacide, 1, the 1/2 of 2-butanediols and water consumption mixes to obtain aqueous mixture；3) aqueous mixture that step 2 obtains is added in the oil mixture that step 1) obtains and is mixed, while supplementing the water of surplus, the disinsection aqueous emulsion of oil-in-water type can be formed.Preparation is environmentally safe using water as matrix, nonflammable, and storing is convenient；Compared with single dose, drug effect height, lasting period length, the generation for having delayed pest resistance to insecticide；Antagonism aphid, wood louse have good control efficiency；Active constituent can degrade in nature, and being compatible with the environment property is good, noresidue, pollution-free, safe and environment-friendly, the features such as to safety of human and livestock.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of containing root bark of celastrus angulatus extract and pepper extract
Novel insecticidal composition and the preparation method and application thereof.
Background technique
In recent years, the weight that high poison, high residue chemical pesticide become pollution of ecological environment, endanger food safety and common people's health
Want problem.With the understanding that people endanger chemical pesticide, country has been limited toxic in World Developed Countries and partial development
The use of chemical pesticide, many countries, which have formulated, reduces the countermeasure that chemical pesticide uses, and proposes the substitute products of chemical pesticide.In
State is a large agricultural country, and pesticide plays highly important effect in the agricultural production in China.Stringent limitation uses chemistry
Pesticide solves excessive pesticide residues, improves the competitiveness demand of the safety and agricultural products in China of agricultural product in international market and compels
It cuts.
To adapt to China's agricultural sustainable development strategy, the developing direction of pesticide will turn to selection from non-selective pesticide
Property pesticide, " the reasonable pesticide of ecology " and " environment harmony pesticide " are turned to from traditional organic chemicals, in favor of protection environment,
Promote the sustainable development of agricultural.Therefore, many biological pesticides are due to having selectivity strong, to safety of human and livestock, to non-target life
The influence of object is smaller, is able to maintain balance of nature；It is easy to degrade in the natural environment, noresidue is free from environmental pollution；It is a variety of because
Element and ingredient play a role, and harmful organism is difficult to the advantages that developing drug resistance and is paid more and more attention (Xu Hanhong, Zhang Zhixiang, journey
The advantages for development of eastern beauty biological pesticide and there are problem world pesticide, 2004,26 (2): 5-10).
Botanical pesticide is one of biological pesticide most ancient in human history, it is from nature, in the natural environment
Degradable, safety nuisance free has become one of green bio pesticide first choice.Phytochemicals production root bark of celastrus angulatus extract comes
Derived from traditional pesticide plant Celastraceae Celastrus Celastrus angulatus (Celastrus angulatus Max.) (Wu Wenjun, 1991).
ABUNDANT NATUREAL RESOURSES, is widely distributed in the mountain area and hills of the Yellow River, the Yangtze river basin, reserves are abundant, according to investigations xuan-en, hubei county
Celastrus angulatus distribution area accounts for the 23.2% of whole county mountainous region area, in Shaanxi Hua County Celastrus angulatus reserves up to the left side 2000 t in terms of dry root skin
The right side, seed at least produces 500 t per year, and Celastrus angulatus can normal growth in clay, sand and rock ballast.According to surveying and determination, average every
100 kg fresh root can obtain 26.2 kg and do root skin, and well developed root system, environment-adapting ability and power of regeneration are all very strong, excavate main root
Second year can sprout many new plant (Wang Yunfeng, Shi Weiyong China's pesticide plant moneys out from the radicula stayed in the soil afterwards
The development and utilization resources science in source, 2001,23(2): 62-64).And its current artificial breeding technology is mature, and (Lu enables
Refined, Nan Yusheng, Ke Zhiguo, Wan Caigan, Xu Huizhu, Wu Dongyun Insecticidal Plant Celastrus Angulatus introduces a fine variety the Hubei study on reproduction preliminary study woods
Industry science and technology, 1987,61 (3): 8-10).
Applicant combines the great demand of international advanced technology and domestic and international market, has been actively developed botanical pesticide ---
Natural root bark of celastrus angulatus extract LS20030503.The natural root bark of celastrus angulatus extract of botanical pesticide to wheat aphid, Paratrioza sinica,
The aphids such as Wolfberry Aphid section insect has certain control efficiency, but its single dose quick-acting is poor, there is slow to pest drug effect,
The defects of field efficacy is general, sprays often, and extension rate is lower.
Therefore, this research department proposes the day for having given efficacy with active constituent group and the research of component compatibility theory, screening
So at grouping, the exploitation of novel plant source pesticide is carried out, can preferably play vegetable source natural active constituent group multicomponent, more
Target spot, synergistic advantage, to solve the problems, such as that botanical pesticide activity is relatively low, quick-acting is poor.
Summary of the invention
The object of the present invention is to provide a kind of good disinsection effect, drug cost is low, noresidue, it is pollution-free, to safety of human and livestock,
It is not likely to produce the Pesticidal combination containing root bark of celastrus angulatus extract and pepper extract of drug resistance, utilizes two kinds of plant source agricultures
The effect of medicine synergy, sufficiently improvement root bark of celastrus angulatus extract quick-acting is poor, and drug effect is slow, knocks down the defects of rate is relatively low,
There is provided preparation method and application simultaneously is another goal of the invention of the invention.
Based on above-mentioned purpose, this invention takes following technical solutions:
A kind of novel insecticidal composition, active constituent are root bark of celastrus angulatus extract and pepper extract.
The weight ratio of root bark of celastrus angulatus extract and pepper extract is 1~8:1~8.
The root bark of celastrus angulatus extract is obtained by following steps: root bark of celastrus angulatus being crushed, root bark of celastrus angulatus powder is obtained, adds
The ethyl alcohol for entering 3~5 times of root bark of celastrus angulatus powder weight is concentrated under reduced pressure after filtering in 60~80 DEG C of 1~4h of ultrasonic extraction to obtain the final product；Institute
It states pepper extract to be obtained by following steps: Chinese prickly ash is crushed, 3~6 times of zanthoxylum powder weight of methanol is added, at 40~70 DEG C
4~6 h of ultrasonic extraction, is concentrated under reduced pressure after filtering to obtain the final product.
The Pesticidal combination is aqueous emulsion, weight percent composition are as follows: active constituent 5~12%, cyclohexanone 3~
30%, sodium benzoate 0.5~1%, ethylene glycol 0.5~1%, 1~3%, 1,2-butanediol 0.5~2% of Butacide, agriculture breast 2201 are 2
~5%, remaining is water.
The weight percent of the aqueous emulsion forms are as follows: root bark of celastrus angulatus extract 8%, pepper extract 4%, cyclohexanone
24%, sodium benzoate 1%, ethylene glycol 1%, Butacide 3%, 1,2-butanediols are 0.6%, agriculture breast 2201 is 5%, remaining is water.
The preparation method of the novel insecticidal composition, comprising the following steps:
1) active constituent is mixed into obtain oil mixture with cyclohexanone；
2) by agriculture breast 2201, sodium benzoate, ethylene glycol, Butacide, 1, the 1/2 of 2-butanediols and water consumption mixes to obtain water
Phase mixture；
3) aqueous mixture that step 2 obtains is added in the oil mixture that step 1) obtains and is mixed, while more than supplement
The water of amount can form the disinsection aqueous emulsion of oil-in-water type.
The specific steps mixed in step 1) and step 2 are as follows: stir 10~30 with the speed of 800~1000 r/min
min；The specific steps mixed in step 3) are as follows: with 10~30 min of high-speed stirred of 10000~12000 r/min.
The application of the Pesticidal combination is applied to the prevention and treatment to Lycium Pests.
In the present invention, each component role are as follows: cyclohexanone is solvent, sodium benzoate is preservative, ethylene glycol is anti-
Jelly agent, Butacide are synergist also known as piperonyl butoxide, 1, and 2-butanediols are stabilizer, agriculture breast 2201 is emulsifier.
Compared with prior art, the beneficial effects of the present invention are:
The present invention be directed to the deficiencies of root bark of celastrus angulatus extract and pepper extract, traditional Chinese medicine theory are introduced, to hardship
Skin rattan root bark extract and pepper extract carry out active constituent group compatibility, to reach the mesh of two kinds of botanical pesticides mutual supplement with each other's advantages
, sufficiently improvement root bark of celastrus angulatus extract quick-acting is poor, and drug effect is slow, knocks down the defects of rate is relatively low.To so far, not
See the report of root bark of celastrus angulatus extract and pepper extract compounding.And the present invention and through multiple repetition test, it achieves following
Good effect；
1, preparation is environmentally safe using water as matrix, nonflammable, and storing is convenient；
2, compared with single dose, drug effect height, lasting period length, the generation for having delayed pest resistance to insecticide；
3, antagonism aphid, wood louse have good control efficiency；
4, active constituent can degrade in nature, and being compatible with the environment property is good, noresidue, pollution-free, safe and environment-friendly, to people
Raise the features such as safe.
Specific embodiment
Below will be by specific embodiment come the present invention will be described, but these specific embodiments are not in any way
It limits the scope of the invention.It should be noted that it is convenient for statement, " bitter green pepper ", which is all made of, in following embodiment carrys out table
Show " root bark of celastrus angulatus extract pepper extract "
The preparation of embodiment 1:9% hardship green pepper aqueous emulsion
A kind of novel insecticidal composition is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract 8%, flower
Green pepper extract 1%, solvent cyclohexanone 18%, preservative sodium benzoate 0.6%, antifreeze glycol 0.7%, synergist Butacide
1.6%, stabilizer 1,2-butanediols 0.6%, the water that emulsifier agriculture breast 2201 is 3% and surplus.
The preparation method of the novel insecticidal composition, comprising the following steps:
1) active constituent is mixed into obtain oil mixture with cyclohexanone；
2) by agriculture breast 2201, sodium benzoate, ethylene glycol, Butacide, 1, the 1/2 of 2-butanediols and water consumption mixes to obtain water
Phase mixture；
3) aqueous mixture that step 2 obtains is added in the oil mixture that step 1) obtains and is mixed, while more than supplement
The water of amount can form the disinsection aqueous emulsion of oil-in-water type.
20 min are stirred with the speed of 900 r/min when mixing in step 1) and step 2；Step 3) mix when
20min is stirred under the high speed of 11000 r/min, while supplementing the water of surplus.In other embodiments, step 1) and step
10~30 min are stirred using the speed of 800~1000 r/min when rapid 2) middle mixing；When step 3) mixes 10000~
10~30min is stirred under the high speed of 12000 r/min.
The root bark of celastrus angulatus extract is obtained by following steps: root bark of celastrus angulatus being crushed, root bark of celastrus angulatus powder is obtained, adds
The ethyl alcohol for entering 4 times of root bark of celastrus angulatus powder weight is concentrated under reduced pressure in 70 DEG C of ultrasonic extraction 2h, after filtering into paste to obtain the final product, described
The mass concentration of ethyl alcohol is 95%；The pepper extract is obtained by following steps: Chinese prickly ash being crushed, is added 5 times of zanthoxylum powder weight
Methanol paste is concentrated under reduced pressure into after filtering in 60 DEG C of 5 h of ultrasonic extraction to obtain the final product.
Embodiment 2:10% hardship green pepper aqueous emulsion
A kind of novel insecticidal composition, be aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract be 8%,
Pepper extract is 2%, solvent cyclohexanone 27%, preservative sodium benzoate 0.8%, antifreeze glycol 0.7%, synergist
Butacide 1.5%, 1,2-butanediol of stabilizer are 0.6%, emulsifier agriculture breast 2201 is 2.5% and surplus is water.
The preparation method of the novel insecticidal composition, difference from Example 1 are: in step 1) and step 2
30 min are stirred with the speed of 800 r/min when mixing；Step 3) stirs under the high speed of 10000 r/min when mixing
30min。
When prepared by the root bark of celastrus angulatus extract, the ethyl alcohol of 3 times of root bark of celastrus angulatus powder weight is added, is mentioned in 60 DEG C of ultrasounds
Take 4h；When prepared by the pepper extract: 3 times of zanthoxylum powder weight of methanol is added, in 40 DEG C of 6 h of ultrasonic extraction.Remaining is the same as real
Apply example 1.
Embodiment 3:12% hardship green pepper aqueous emulsion
A kind of novel insecticidal composition, be aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract be 8%,
Pepper extract is 4%, solvent cyclohexanone 24%, preservative sodium benzoate 1%, antifreeze glycol 1%, synergist synergy
Ether 3%, 1,2-butanediol of stabilizer are 0.6%, emulsifier agriculture breast 2201 is 5% and surplus is water.
The preparation method of the novel insecticidal composition, difference from Example 1 are: in step 1) and step 2
10 min are stirred with the speed of 1000 r/min when mixing；Step 3) stirs under the high speed of 12000 r/min when mixing
10min。
When prepared by the root bark of celastrus angulatus extract: the ethyl alcohol of 5 times of root bark of celastrus angulatus powder weight is added, is mentioned in 80 DEG C of ultrasounds
Take 1h；When prepared by the pepper extract: 6 times of zanthoxylum powder weight of methanol is added, in 70 DEG C of 4 h of ultrasonic extraction.Remaining is the same as real
Apply example 1.
Embodiment 4:10% hardship green pepper aqueous emulsion
A kind of novel insecticidal composition, be aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract be 5%,
Pepper extract is 5%, solvent cyclohexanone 30%, preservative sodium benzoate 0.9%, antifreeze glycol 0.8%, synergist
Butacide 2.5%, 1,2-butanediol of stabilizer are 1.6%, emulsifier agriculture breast 2201 is 3.5% and surplus is water.
The preparation method is the same as that of Example 1 for the novel insecticidal composition.
Embodiment 5:6% hardship green pepper aqueous emulsion
A kind of novel insecticidal composition, be aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract be 2%,
Pepper extract is 4%, solvent cyclohexanone 9%, preservative sodium benzoate 0.6%, antifreeze glycol 0.6%, synergist
Butacide 1.5%, 1,2-butanediol of stabilizer are 0.6%, emulsifier agriculture breast 2201 is 3% and surplus is water.
The preparation method is the same as that of Example 1 for the novel insecticidal composition.
Embodiment 6:7.5% hardship green pepper aqueous emulsion
A kind of novel insecticidal composition is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract 1.5%,
Pepper extract 6%, solvent cyclohexanone 15%, preservative sodium benzoate 0.6%, antifreeze glycol 0.6%, synergist Butacide
1.2%, stabilizer 1,2-butanediols 0.6%, emulsifier agriculture breast 2201 are 3.5% and surplus is water.
The preparation method is the same as that of Example 1 for the novel insecticidal composition.
Embodiment 7:10.8% hardship green pepper aqueous emulsion
A kind of novel insecticidal composition is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract is
1.2%, pepper extract 9.6%, solvent cyclohexanone 25%, preservative sodium benzoate 0.9%, antifreeze glycol 1%,
Synergist Butacide 2.8%, 1,2-butanediol of stabilizer are 1.8%, emulsifier agriculture breast 2201 is 4.5% and surplus is water.
Embodiment 8:5% hardship green pepper aqueous emulsion
A kind of novel insecticidal composition, be aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract be 2%,
Pepper extract is 3%, solvent cyclohexanone 3%, preservative sodium benzoate 0.5%, antifreeze glycol 0.5%, synergist
Butacide 1%, 1,2-butanediol of stabilizer are 0.5%, emulsifier agriculture breast 2201 is 2% and surplus is water.
The preparation method is the same as that of Example 1 for the novel insecticidal composition.
9 effect experiment of embodiment
For the technical effect for verifying products obtained therefrom of the present invention, inventor has carried out multiple repetition test, specific as follows.
Experimental method: root bark of celastrus angulatus extract and pepper extract proportion respectively 8:1,4:1,2:1,1:1,1:2,1:
4, the extracting method of 1:8, root bark of celastrus angulatus extract and pepper extract is with above-described embodiment 1, and each proportion is respectively with distillation
Water is diluted to 5 concentration, and every processing is repeated 4 times, and sets blank control.
9.1 pairs of Wolfberry Aphid indoor toxicity tests:
Test worm processing uses infusion process.The fresh fructus lycii tender tip with aphid is adopted, winged aphids and extra aphid are rejected,
Keep aphid size almost the same, then it is impregnated 5 s in medical fluid and is put into after taking-up is dried and has been lined with filter paper by medium density
Diameter be in the culture dish of 9 cm, culture dish adds water moisturizing, be placed in RXZ intelligence artificial lighting climate box, temperature (25 ±
0.5) DEG C, relative humidity (RH) 60%, illumination L: D are 16 h: 8h, the dead borer population of each processing are checked after 24 h, and calculate correction extremely
Die rate.Death standard touches aphid body with writing brush, and with aphid foot, inactive person is death (Du Yuning, Zhang Zongshan, Shen Ruiqing, Koryo
Indoor toxicity test [J] the forestry science and technology of 4 kinds of biological pesticides to Wolfberry Aphid, 2007,32 (6): 29-30).
The gained death rate is corrected with Abbott formula, according to drug concentration logarithm and corrected mortality probit value
Return law of the straight line, calculate virulence regression equation, the lethal concentration of 50 (LC50).Mixture is found out using (1960) formula such as Sun Yunpei
Co-toxicity coefficient (CTC) (Sun Y P, E R Johnnson. Analysis of joint action of pesticides
Against house files [J] Ecological Entomology, 1960,53:887-891).Synergy judgement mark
Quasi-: it is significant synergy that co-toxicity coefficient (CTC), which is greater than 200, and 150~200 is smaller for synergistic effect, and 70~150 be summation action, small
In 70 for antagonism (Tan Fujie, Shen Jinliang, You Ziping insecticide mixing application research [J] Agricultural University Of Nanjing report,
1987,4 (increasings): 93-99).
In formula: A indicates A single dose toxicity index；ACIndicate A shared percentage in mixture；B indicates B single dose toxicity index；
BCIndicate B shared percentage in mixture.
The virulence of root bark of celastrus angulatus extract and pepper extract to Wolfberry Aphid together be shown in by malicious coefficient determination calculated result
Table 1:
1 root bark of celastrus angulatus extract of table and pepper extract are to the virulence of Wolfberry Aphid malicious coefficient determination result together
。
The measurement result of table 1 shows: root bark of celastrus angulatus extract and pepper extract two kinds of active components group are by table 1
When having significant synergistic effect, root bark of celastrus angulatus extract and pepper extract 2:1 to Wolfberry Aphid after ratio compounding, synergy is made
With most strong, co-toxicity coefficient 272.84；When ratio is 8:1,4:1,1:1,1:2,1:4,1:8, co-toxicity coefficient is followed successively by 173.43,
223.24、211.46、204.24、181.47、142.55。
9.2 pairs of Paratrioza sinica indoor toxicity tests:
The wolfberry leaf with wood louse is taken, the wood louse similar in writing brush picking individual size is uniformly placed on face of blade
And each small on piece is made to have wood louse 20~30, blade is immersed in prepared pesticide solution, is taken out after impregnating 5 s,
Extra medical fluid is sucked with blotting paper and is dried, and is then placed in the culture dish for spreading 90 mm of the wet water absorbent paper that haves three layers, it is wet with 2 layers
Culture dish lid is covered after gauze sealing, room temperature is maintained at (26 ± 1) DEG C.Every processing is repeated 3 times, and is control with clear water.
Total borer population and dead borer population are investigated after 24 d.Death standard is to touch polypide and appendage with small writing brush, and no any reaction is then considered as dead
Die (Guan Xiaoqing.The screening and natural enemy Study of Sensitivity of Paratrioza sinica biological agent.Agriculture of Anhui science, 2009,37(10):
4541-4542).Calculate virulence regression equation, the lethal concentration of 50 (LC50) and co-toxicity coefficient.
The virulence of root bark of celastrus angulatus extract and pepper extract to Paratrioza sinica together be shown in by malicious coefficient determination calculated result
Table 2.
2 root bark of celastrus angulatus extract of table and pepper extract are to the virulence of Paratrioza sinica malicious coefficient determination result together
。
The measurement result of table 2 shows: root bark of celastrus angulatus extract and pepper extract two kinds of active components group are by table 2
When having significant synergistic effect, root bark of celastrus angulatus extract and pepper extract 2:1 to Paratrioza sinica after ratio compounding, synergy is made
With most strong, co-toxicity coefficient 280.34；When ratio is 8:1,4:1,1:1,1:2,1:4,1:8, co-toxicity coefficient is followed successively by 165.75,
221.61、206.03、197.23、172.38、132.29。
9.3 embodiments 1,3,4,6 prevent and treat Wolfberry Aphid field control effectiveness test:
Test is divided into two batches progress, and setting embodiment 1 and embodiment 4 respectively is first group, and embodiment 3 and embodiment 6 are the
Two groups, and the control sample of each embodiment is prepared respectively, wherein the control sample of embodiment 1 is 8% root bark of celastrus angulatus extract and 1% flower
Green pepper extract, the control sample of embodiment 3 are 8% root bark of celastrus angulatus extract and 4% pepper extract, and the control sample of embodiment 4 is
10% root bark of celastrus angulatus extract and 5% pepper extract, the control sample of embodiment 6 are 1.5% root bark of celastrus angulatus extract and 6% flower
Green pepper extract, while using clear water as blank control.Wherein the specific preparation method of each control sample is referring to its corresponding each embodiment,
Unlike, the active constituent in each embodiment is replaced with to the active constituent of corresponding control sample, such as the control of embodiment 1
Sample is respectively 8% root bark of celastrus angulatus extract and 1% pepper extract, and 8% root bark of celastrus angulatus extract of control sample is then by embodiment
In active constituent root bark of celastrus angulatus extract and pepper extract replace with root bark of celastrus angulatus extract, other components are constant,
Water supplement makes active constituent root bark of celastrus angulatus extractive content 8%.
It tests and is carried out in July, 2014 in lycium barbarum production base.Triennial fructus lycii seedling is selected, in Wolfberry Aphid 1st generation children
Nymph age, peak period beginning is sprayed with Backpack type mechanical mist and powder spraying machine.Every processing is repeated 3 times, the arrangement of completely random district's groups, and sets guarantor
Shield row.50kg water/667m2, after selecting fine day 16:00, configured medicament implementation complete stool is sprayed, emphasis sprays tree crown taper
And leave dual sides, accomplish uniform, careful, comprehensive covering.Every cell selects 10 plants of trees, selection aphid is more in each tree 2
A branch is marked as fixed sample branch with label, indicates number, medicament title, concentration.The worm on fixed sample branch is investigated before application
Mouth radix.After spray 3 d, 7 d, 15 d investigate respectively on the fixed sample branch of every cell work aphid number (Guo Rui, Yan Lin, Jin Shengying,
Chen Shengcui DS, which is enabled, breathes out Wolfberry Aphid toxicity test and the north field control effectiveness test gardening, and 2012, (10): 148-151).
Data analysis is carried out using SPSS22.0 software.It the results are shown in Table 3 and table 4.
Control efficiency is the data by efficacy survey compared with blank district, is calculated control efficiency (Revision insect recluced rate),
Calculation formula is as follows:
；
。
3 first groups of embodiment preparations of table prevent and treat Wolfberry Aphid field control effectiveness test
。
4 second groups of embodiment preparations of table prevent and treat Wolfberry Aphid field control effectiveness test
。
The preparation of two kinds of active components group compounding of the present invention, prevents and treats the drug effect table 3 as above and table 4 of Wolfberry Aphid, it is known that, this
The preparation of invention two kinds of active components group compounding significantly improves Wolfberry Aphid control efficiency, hence it is evident that is better than two kinds of active components group
It is used alone, and has preferable quick-acting.Treatment agent is the extension rate of 9%, 10% and 12% bitter 500 times of green pepper, and 3d's is anti-
Effect is 72%~97%；When root bark of celastrus angulatus extract and pepper extract ratio are 2:1 (embodiment 3), preventive effect is best,
3d, 7d, 15d after processing, preventive effect are up to 95.23%, 98.07% and 98.06% respectively, and to target in this test scope of medication
It is denoted as object and beneficial organism safety.
9.4 embodiments 1,3,4,6 prevent and treat Paratrioza sinica field control effectiveness test:
Test is divided into two batches progress, and setting embodiment 1 and embodiment 4 respectively is first group, and embodiment 3 and embodiment 6 are the
Two groups, and the control sample of each embodiment is prepared respectively, control sample is specifically prepared referring to 9.3, while using clear water as blank control.Examination
It tests and is carried out in July, 2014 in lycium barbarum production base.Every kind of medicament sets up 2 concentration processing separately, is control with clear water.Often
3 plants of trees are chosen in processing, and random district's groups arrangement is repeated 3 times, each plant is sufficiently sprayed up to not with hand-powered knapsack sprayer
Dropping liquid is advisable.Check Paratrioza sinica radix is adjusted before application, respectively listing mark.3d, 5d, 7d are investigated on every after application
Wood louse number calculates Paratrioza sinica Revision insect recluced rate and control efficiency (Chen Peimin, Wang Jianping, Zhong Yanping, Su Huiming, tension army
The Inner Mongol several plant source chemical control Paratrioza sinica pharmacodynamic test agricultural science and technology, 2013(5): 66-67).It the results are shown in Table 5
With shown in table 6.
5 first groups of embodiment preparations of table prevent and treat Paratrioza sinica field efficacy
。
6 second groups of embodiment preparations of table prevent and treat Paratrioza sinica field efficacy
。
The preparation of two kinds of active components group compounding of the present invention, prevents and treats the drug effect table 5 as above and table 6 of Paratrioza sinica, it is known that, this
The preparation of invention two kinds of active components group compounding significantly improves Paratrioza sinica control efficiency, hence it is evident that is better than two kinds of active components group
It is used alone, and has preferable quick-acting.Treatment agent is the extension rate of 9%, 10% and 12% bitter 500 times of green pepper, and 3d's is anti-
Effect is 70%~97%；When root bark of celastrus angulatus extract and pepper extract ratio are 2:1 (embodiment 3), preventive effect is best,
3 d, 7 d, 15 d after processing, preventive effect are up to 96.78%, 97.73% and 98.10% respectively.
In addition, we carry out root bark of celastrus angulatus extract and the most significant proportion 2:1 of pepper extract synergistic effect
The acute toxicity test of small white mouse, regression equation are Y=- 1.13+1.61x, acute oral LD50Value is 6413.23mg/kg, micro-
In malicious range.
In conclusion Pesticidal combination of the invention, which has, enhances Wolfberry Aphid and Paratrioza sinica control efficiency, it is quick-acting
Property the long advantage of good, lasting effect in addition to significant insecticidal effect, can effectively be dropped compared with existing single formulation
Low dispenser cost, slows down the generation of resistance, reduces preparation residual quantity, pollution-free to crop, safety is good, meets pesticidal preparations
Security requirement.
Claims (4)
1. a kind of novel insecticidal composition, which is characterized in that its active constituent is root bark of celastrus angulatus extract and pepper extract；
The weight ratio of root bark of celastrus angulatus extract and pepper extract is 1~8:1~8；The Pesticidal combination is aqueous emulsion, weight
Percentage composition are as follows: active constituent 5~12%, cyclohexanone 3~30%, sodium benzoate 0.5~1%, ethylene glycol 0.5~1%, Butacide
1~3%, 1,2-butanediols 0.5~2%, agriculture breast 2201 are 2~5%, remaining is water；The root bark of celastrus angulatus extract is by following
Step obtains: root bark of celastrus angulatus crushed, root bark of celastrus angulatus powder is obtained, the ethyl alcohol of 3~5 times of root bark of celastrus angulatus powder weight is added,
60~80 DEG C of 1~4h of ultrasonic extraction, are concentrated under reduced pressure after filtering to obtain the final product；The pepper extract is obtained by following steps: by Chinese prickly ash
It crushes, 3~6 times of zanthoxylum powder weight of methanol is added and is concentrated under reduced pressure after filtering in 40~70 DEG C of 4~6 h of ultrasonic extraction to obtain the final product.
2. novel insecticidal composition as described in claim 1, which is characterized in that the weight percent of the aqueous emulsion forms
Are as follows: root bark of celastrus angulatus extract 8%, pepper extract 4%, cyclohexanone 24%, sodium benzoate 1%, ethylene glycol 1%, Butacide
3%, 1,2-butanediols are 0.6%, agriculture breast 2201 is 5%, remaining is water.
3. the preparation method of any novel insecticidal composition of claim 1-2, which comprises the following steps:
1) active constituent is mixed into obtain oil mixture with cyclohexanone；
2) by agriculture breast 2201, sodium benzoate, ethylene glycol, Butacide, 1, the 1/2 of 2-butanediols and water consumption mix water phase is mixed
Close object；
3) aqueous mixture that step 2 obtains is added in the oil mixture that step 1) obtains and is mixed, while supplementing surplus
Water can form the disinsection aqueous emulsion of oil-in-water type.
4. the preparation method of novel insecticidal composition as claimed in claim 3, which is characterized in that mixed in step 1) and step 2
The specific steps of conjunction are as follows: 10~30 min are stirred with the speed of 800~1000 r/min；The specific steps mixed in step 3)
Are as follows: with 10~30 min of high-speed stirred of 10000~12000 r/min.
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