CN106689225A - Novel insecticidal composition and preparation method and application thereof - Google Patents
Novel insecticidal composition and preparation method and application thereof Download PDFInfo
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- CN106689225A CN106689225A CN201611128516.XA CN201611128516A CN106689225A CN 106689225 A CN106689225 A CN 106689225A CN 201611128516 A CN201611128516 A CN 201611128516A CN 106689225 A CN106689225 A CN 106689225A
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- 239000000203 mixture Substances 0.000 title claims abstract description 66
- 230000000749 insecticidal effect Effects 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 241000296609 Celastrus angulatus Species 0.000 claims abstract description 69
- 239000000284 extract Substances 0.000 claims abstract description 59
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims abstract description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 41
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000000839 emulsion Substances 0.000 claims abstract description 24
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 17
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 17
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 17
- BMRWNKZVCUKKSR-UHFFFAOYSA-N butane-1,2-diol Chemical compound CCC(O)CO BMRWNKZVCUKKSR-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 14
- FIPWRIJSWJWJAI-UHFFFAOYSA-N Butyl carbitol 6-propylpiperonyl ether Chemical compound C1=C(CCC)C(COCCOCCOCCCC)=CC2=C1OCO2 FIPWRIJSWJWJAI-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229960005235 piperonyl butoxide Drugs 0.000 claims abstract description 12
- 230000000361 pesticidal effect Effects 0.000 claims abstract description 8
- 230000001502 supplementing effect Effects 0.000 claims abstract description 5
- 229940116257 pepper extract Drugs 0.000 claims description 35
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- 210000000481 breast Anatomy 0.000 claims description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 15
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000002137 ultrasound extraction Methods 0.000 claims description 8
- 241000949456 Zanthoxylum Species 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 3
- 241001124076 Aphididae Species 0.000 abstract description 24
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- 230000002265 prevention Effects 0.000 abstract description 9
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- 239000003795 chemical substances by application Substances 0.000 abstract description 6
- 241000607479 Yersinia pestis Species 0.000 abstract description 3
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a novel insecticidal composition which comprises the active ingredients of a celastrus angulatus root extract and a Chinese prickly ash extract. The preparation method comprises the following steps of (1) mixing the active ingredients with cyclohexanone to obtain an oil-phase mixture; (2) mixing a pesticide emulsifier 2201, sodium benzoate, ethylene glycol, piperonyl butoxide, 1,2-butanediol and 1/2 of water to obtain a water-phase mixture; and (3) adding the water-phase mixture obtained in the step (2) into the oil-phase mixture obtained in the step (1), and simultaneously supplementing the balance of water to form an oil-in-water type insecticidal water emulsion. Water is taken as a matrix, so that the preparation is safe to the environment, not easy to combust and convenient to store and transport; compared with a single agent, the novel insecticidal composition has the advantages of high pesticide effect and long lasting period and the pest resistance to insecticides is delayed; the novel insecticidal composition has good prevention and treatment effects on resistant aphids and phylloxera; and the active ingredients can be degraded in nature, so that the insecticidal composition has the characteristics of being good in environmental compatibility, free of residue, free of pollution, safe, environment-friendly and safe to people and animals.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of containing root bark of celastrus angulatus extract and pepper extract
Novel insecticidal composition and preparation method and application.
Background technology
In recent years, high poison, high residue chemical pesticide turn into the weight of pollution of ecological environment, harm food security and common people's health
Want problem.With the understanding that people are endangered chemical pesticide, country has limited poisonous in World Developed Countries and partial development
The use of chemical pesticide, many countries have formulated and have reduced the countermeasure that chemical pesticide is used, and propose the substitute products of chemical pesticide.In
Guo Shiyige large agricultural countries, agricultural chemicals plays highly important effect in the agricultural production of China.Strict limitation uses chemistry
Agricultural chemicals, solution residues of pesticides are exceeded, improve the competitiveness demand of the security and agricultural products in China of agricultural product in international market and compel
Cut.
To adapt to China's agricultural sustainable development strategy, the developing direction of agricultural chemicals will turn to select from non-selective agricultural chemicals
Property agricultural chemicals, turn to " ecological rationally agricultural chemicals " and " the harmonious agricultural chemicals of environment " from traditional organic chemicals, be beneficial to environmental protection,
Promote the sustainable development of agricultural.Therefore, many biological pesticides are due to strong with selectivity, to person poultry safety, to non-target life
The influence of thing is smaller, can keep balance of nature；It is easy to degraded in natural environment, noresidue is free from environmental pollution；It is various because
Element and composition play a role, and harmful organism is difficult to the advantages of developing immunity to drugs and is increasingly subject to pay attention to (Xu Hanhong, Zhang Zhixiang, journey
The advantages for development of eastern U.S.'s biological pesticides and there is problem world pesticides, 2004,26 (2)：5-10).
Botanical pesticide is one of biological pesticide most ancient in human history, and it is from nature, in natural environment
Degradable, safety nuisance free, as one of green bio agricultural chemicals first-selection.Phytochemicals production root bark of celastrus angulatus extract comes
Come from traditional pesticide plant Celastraceae Celastrus Celastrus angulatus (Celastrus angulatus Max.) (Wu Wenjun, 1991).
ABUNDANT NATUREAL RESOURSES, is widely distributed in the mountain area and hills of the Yellow River, the Yangtze river basin, reserves enrich, according to investigations xuan-en, hubei county
Celastrus angulatus distribution area accounts for the 23.2% of whole county mountain region area, left up to 2000 t in terms of dry root skin in Shaanxi Hua County Celastrus angulatus reserves
The right side, seed at least produces 500 t per year, and Celastrus angulatus can normal growth in clay, sand and rock ballast.According to surveying and determination, it is average every
100 kg fresh quench can obtain the dry root skins of 26.2 kg, and its well developed root system, environment-adapting ability and power of regeneration are all very strong, excavate main root
Second Year can sprout many new plant from the radicula stayed in soil afterwards（Wang Yunfeng, the big brave China pesticide plant money of stone
The exploitation resources sciences in source, 2001,23（2）：62-64）.And current its artificial breeding technology is ripe, and (Lu makes
Refined, Nan Yusheng, Ke Zhiguo, Wan Caigan, Xu Huizhu, Wu's winter cloud Insecticidal Plant Celastrus Angulatus introduce a fine variety study on reproduction preliminary study Hubei woods
Industry science and technology, 1987,61 (3)：8-10).
Applicant combines the great demand of international advanced technology and domestic and international market, has been actively developed botanical pesticide ---
Natural root bark of celastrus angulatus extract LS20030503.The natural root bark of celastrus angulatus extract of botanical pesticide to wheat aphid, Paratrioza sinica,
The aphid such as Wolfberry Aphid section insect has certain prevention effect, but its single dose quick-acting is poor, exists to insect drug effect slowly,
Field efficacy is general, sprays often, the more low defect of extension rate.
Therefore, this research department is proposed with the day of active component group and the research of component compatibility theory, screening with given efficacy
So into packet, the exploitation of novel plant source pesticide is carried out, can preferably play vegetable source natural active component group multicomponent, many
Target spot, Synergistic advantage, so as to solve the problem that botanical pesticide activity is relatively low, quick-acting is poor.
The content of the invention
It is an object of the invention to provide a kind of good disinsection effect, low drug cost, noresidue, it is pollution-free, to person poultry safety,
The drug-fast Pesticidal combination containing root bark of celastrus angulatus extract and pepper extract is not likely to produce, using two kinds of plant source agricultures
Medicine synergy is acted on, and fully improves that root bark of celastrus angulatus extract quick-acting is poor, and drug effect slowly, knocks down the defects such as speed is relatively low,
It is another goal of the invention of the invention that its preparation method is provided simultaneously with application.
Based on above-mentioned purpose, this invention takes following technical scheme：
A kind of novel insecticidal composition, its active component is root bark of celastrus angulatus extract and pepper extract.
The weight ratio of root bark of celastrus angulatus extract and pepper extract is 1~8:1~8.
The root bark of celastrus angulatus extract is obtained by following steps：Root bark of celastrus angulatus is crushed, root bark of celastrus angulatus powder is obtained, plus
Enter the ethanol of 3~5 times of root bark of celastrus angulatus powder weight, be concentrated under reduced pressure in 60~80 DEG C of 1~4h of ultrasonic extraction, after filtering and obtained final product；Institute
Pepper extract is stated to be obtained by following steps：Chinese prickly ash is crushed, the methyl alcohol of 3~6 times of zanthoxylum powder weight is added, at 40~70 DEG C
The h of ultrasonic extraction 4~6, is concentrated under reduced pressure after filtering and obtains final product.
The Pesticidal combination is aqueous emulsion, and its percentage by weight composition is：Active component 5~12%, cyclohexanone 3~
30%th, Sodium Benzoate 0.5~1%, ethylene glycol 0.5~1%, Butacide 1~3%, 1,2-butanediol 0.5~2%, agriculture breast 2201 are 2
~5%, remaining is water.
The percentage by weight of the aqueous emulsion is constituted：Root bark of celastrus angulatus extract 8%, pepper extract 4%, cyclohexanone
24%th, Sodium Benzoate is that 1%, ethylene glycol is 1%, Butacide 3%, 1, and 2-butanediol is that 0.6%, agriculture breast 2201 is 5%, and remaining is water.
The preparation method of described novel insecticidal composition, comprises the following steps：
1）Active component is mixed into obtain oil mixture with cyclohexanone；
2）By agriculture breast 2201, Sodium Benzoate, ethylene glycol, Butacide, 1,2-butanediol with the 1/2 of water consumption mix water is mutually mixed
Compound；
3）By step 2）The aqueous mixture for obtaining adds step 1）Mix in the oil mixture for obtaining, while supplementing surplus
Water, you can form the disinsection aqueous emulsion of oil-in-water type.
Step 1）With step 2）Middle mixing is concretely comprised the following steps：10~30 are stirred with the speed of 800~1000 r/min
min；Step 3）Middle mixing is concretely comprised the following steps：With the min of high-speed stirred 10~30 of 10000~12000 r/min.
The application of described Pesticidal combination, is applied to the preventing and treating to Lycium Pests.
In the present invention, the role of each component is：Cyclohexanone is solvent, Sodium Benzoate is preservative, ethylene glycol is anti-
It is synergist to freeze agent, Butacide, also known as piperonyl butoxide, 1, and 2-butanediol is that stabilizer, agriculture breast 2201 are emulsifying agent.
Compared with prior art, the beneficial effects of the present invention are：
The present invention be directed to root bark of celastrus angulatus extract and the deficiency of pepper extract, traditional Chinese medicine theory is introduced, to Celastrus angulatus
Root bark extract and pepper extract carry out active component and assemble 5, to reach the purpose that two kinds of botanical pesticides have complementary advantages,
Fully improve root bark of celastrus angulatus extract quick-acting poor, drug effect slowly, knocks down the defects such as speed is relatively low.To so far, have no
The report of root bark of celastrus angulatus extract and pepper extract compounding.And the present invention and through multiple repetition test, achieve following good
Good effect；
1st, preparation is environmentally safe with water as matrix, nonflammable, and storing is convenient；
2nd, compared with single dose, drug effect is high, the lasting period is long, delayed the generation of pest resistance to insecticide；
3rd, antagonism aphid, wood louse have good prevention effect；
4th, active component can be degraded in nature, and being compatible with the environment property is good, noresidue, pollution-free, safe and environment-friendly, and people and animals are pacified
Congruent feature.
Specific embodiment
Below will be by specific embodiment come the present invention will be described, but these specific embodiments are never in any form
Limit the scope of the invention.It should be noted that for statement is convenient, using " bitter green pepper " come table in following embodiment
Show " root bark of celastrus angulatus extract pepper extract "
Embodiment 1：The preparation of 9% bitter green pepper aqueous emulsion
A kind of novel insecticidal composition, is aqueous emulsion, and its percentage by weight composition is：Root bark of celastrus angulatus extract 8%, Chinese prickly ash is carried
Take thing 1%, solvent cyclohexanone 18%, preservative sodium benzoate 0.6%, antifreeze glycol 0.7%, synergist Butacide 1.6%, steady
Determine agent 1,2-butanediol 0.6%, emulsifying agent agriculture breast 2201 are the water of 3% and surplus.
The preparation method of described novel insecticidal composition, comprises the following steps：
1）Active component is mixed into obtain oil mixture with cyclohexanone；
2）By agriculture breast 2201, Sodium Benzoate, ethylene glycol, Butacide, 1,2-butanediol with the 1/2 of water consumption mix water is mutually mixed
Compound；
3）By step 2）The aqueous mixture for obtaining adds step 1）Mix in the oil mixture for obtaining, while supplementing surplus
Water, you can form the disinsection aqueous emulsion of oil-in-water type.
Step 1）With step 2）20 min are stirred with the speed of 900 r/min during middle mixing；Step 3）During mixing
20min is stirred under the high speed of 11000 r/min, while supplementing the water of surplus, you can.In other embodiments, step 1）And step
Rapid 2）Speed during middle mixing using 800~1000 r/min stirs 10~30 min；Step 3）During mixing 10000~
10~30min is stirred under the high speed of 12000 r/min.
The root bark of celastrus angulatus extract is obtained by following steps：Root bark of celastrus angulatus is crushed, root bark of celastrus angulatus powder is obtained, plus
Enter the ethanol of 4 times of root bark of celastrus angulatus powder weight, in 70 DEG C of ultrasonic extraction 2h, paste is concentrated under reduced pressure into after filtering and is obtained final product, it is described
The mass concentration of ethanol is 95%；The pepper extract is obtained by following steps：Chinese prickly ash is crushed, 5 times of zanthoxylum powder weight is added
Methyl alcohol, in 60 DEG C of h of ultrasonic extraction 5, paste is concentrated under reduced pressure into after filtering and is obtained final product.
Embodiment 2：10% bitter green pepper aqueous emulsion
A kind of novel insecticidal composition, is aqueous emulsion, and its percentage by weight composition is：Root bark of celastrus angulatus extract is 8%, Chinese prickly ash
Extract is that 2%, solvent cyclohexanone is that 27%, preservative sodium benzoate is that 0.8%, antifreeze glycol is 0.7%, synergist synergy
Ether 1.5%, stabilizer 1,2-butanediol are that 0.6%, emulsifying agent agriculture breast 2201 is 2.5% and balance of water.
The preparation method of described novel insecticidal composition, difference from Example 1 is：Step 1）With step 2）In
30 min are stirred with the speed of 800 r/min during mixing；Step 3）Stirred under the high speed of 10000 r/min during mixing
30min。
When prepared by the root bark of celastrus angulatus extract, the ethanol of 3 times of root bark of celastrus angulatus powder weight is added, carried in 60 DEG C of ultrasounds
Take 4h；When prepared by the pepper extract：The methyl alcohol of 3 times of zanthoxylum powder weight is added, in 40 DEG C of h of ultrasonic extraction 6.Remaining is with real
Apply example 1.
Embodiment 3：12% bitter green pepper aqueous emulsion
A kind of novel insecticidal composition, is aqueous emulsion, and its percentage by weight composition is：Root bark of celastrus angulatus extract is 8%, Chinese prickly ash
Extract is that 4%, solvent cyclohexanone is that 24%, preservative sodium benzoate is that 1%, antifreeze glycol is 1%, synergist Butacide
3%th, stabilizer 1,2-butanediol is that 0.6%, emulsifying agent agriculture breast 2201 is 5% and balance of water.
The preparation method of described novel insecticidal composition, difference from Example 1 is：Step 1）With step 2）In
10 min are stirred with the speed of 1000 r/min during mixing；Step 3）Stirred under the high speed of 12000 r/min during mixing
10min。
When prepared by the root bark of celastrus angulatus extract：The ethanol of 5 times of root bark of celastrus angulatus powder weight is added, is carried in 80 DEG C of ultrasounds
Take 1h；When prepared by the pepper extract：The methyl alcohol of 6 times of zanthoxylum powder weight is added, in 70 DEG C of h of ultrasonic extraction 4.Remaining is with real
Apply example 1.
Embodiment 4：10% bitter green pepper aqueous emulsion
A kind of novel insecticidal composition, is aqueous emulsion, and its percentage by weight composition is：Root bark of celastrus angulatus extract is 5%, Chinese prickly ash
Extract is that 5%, solvent cyclohexanone is that 30%, preservative sodium benzoate is that 0.9%, antifreeze glycol is 0.8%, synergist synergy
Ether 2.5%, stabilizer 1,2-butanediol are that 1.6%, emulsifying agent agriculture breast 2201 is 3.5% and balance of water.
The preparation method of described novel insecticidal composition is with embodiment 1.
Embodiment 5：6% bitter green pepper aqueous emulsion
A kind of novel insecticidal composition, is aqueous emulsion, and its percentage by weight composition is：Root bark of celastrus angulatus extract is 2%, Chinese prickly ash
Extract is that 4%, solvent cyclohexanone is that 9%, preservative sodium benzoate is that 0.6%, antifreeze glycol is 0.6%, synergist synergy
Ether 1.5%, stabilizer 1,2-butanediol are that 0.6%, emulsifying agent agriculture breast 2201 is 3% and balance of water.
The preparation method of described novel insecticidal composition is with embodiment 1.
Embodiment 6：7.5% bitter green pepper aqueous emulsion
A kind of novel insecticidal composition, is aqueous emulsion, and its percentage by weight composition is：Root bark of celastrus angulatus extract 1.5%, Chinese prickly ash
Extract 6%, solvent cyclohexanone 15%, preservative sodium benzoate 0.6%, antifreeze glycol 0.6%, synergist Butacide 1.2%,
Stabilizer 1,2-butanediol 0.6%, emulsifying agent agriculture breast 2201 are 3.5% and balance of water.
The preparation method of described novel insecticidal composition is with embodiment 1.
Embodiment 7：10.8% bitter green pepper aqueous emulsion
A kind of novel insecticidal composition, is aqueous emulsion, and its percentage by weight composition is：Root bark of celastrus angulatus extract is 1.2%, flower
Green pepper extract is that 9.6%, solvent cyclohexanone is that 25%, preservative sodium benzoate is that 0.9%, antifreeze glycol is that 1%, synergist increases
Effect ether 2.8%, stabilizer 1,2-butanediol are that 1.8%, emulsifying agent agriculture breast 2201 is 4.5% and balance of water.
Embodiment 8：5% bitter green pepper aqueous emulsion
A kind of novel insecticidal composition, is aqueous emulsion, and its percentage by weight composition is：Root bark of celastrus angulatus extract is 2%, Chinese prickly ash
Extract is that 3%, solvent cyclohexanone is that 3%, preservative sodium benzoate is that 0.5%, antifreeze glycol is 0.5%, synergist synergy
Ether 1%, stabilizer 1,2-butanediol are that 0.5%, emulsifying agent agriculture breast 2201 is 2% and balance of water.
The preparation method of described novel insecticidal composition is with embodiment 1.
The effect experiment of embodiment 9
To verify the technique effect of products obtained therefrom of the present invention, inventor has carried out multiple repetition test, specific as follows.
Experimental technique：Root bark of celastrus angulatus extract and pepper extract proportioning are respectively 8:1、4:1、2:1、1:1、1:2、1:
4、1:8, with above-described embodiment 1, each proportioning is respectively with distillation for the extracting method of root bark of celastrus angulatus extract and pepper extract
Water is diluted to 5 concentration, and often treatment is repeated 4 times, and sets blank.
9.1 pairs of Wolfberry Aphid Toxicity Determinations：
Test worm treatment uses infusion process.The fresh matrimony vine tender tip with aphid is adopted, alatae worm and unnecessary aphid is rejected, makes aphid
Worm size is basically identical, medium density, and then it impregnated 5 s in liquid, after taking-up is dried, is put into and has been lined with the straight of filter paper
During footpath is the culture dish of 9 cm, culture dish adds water moisturizing, inserts in RXZ intelligence artificial lighting climate boxs, temperature (25 ± 0.5)
DEG C, relative humidity (RH) 60%, illumination L: D is 16 h: 8h, and the dead borer population of each treatment is checked after 24 h, and it is dead to calculate correction
Rate.Death standard touches aphid body with writing brush, and with aphid, the inactive person of foot is death（Du Yuning, Zhang Zongshan, Shen Ruiqing, Koryo 4
Plant Toxicity Determination [J] the forestry science and technologies of biological pesticide to Wolfberry Aphid, 2007,32 (6):29-30）.
The gained death rate is corrected with Abbott formula, according to drug concentration logarithm value and corrected mortality probit value
Return law of the straight line, calculate virulence regression equation, the lethal concentration of 50 (LC50).Mixture is obtained using (1960) formula such as Sun Yunpei
Co-toxicity coefficient (CTC) (Sun Y P, E R Johnnson. Analysis of joint action of pesticides
Against house files [J] Ecological Entomology, 1960,53: 887-891).Synergy judges mark
It is accurate：Co-toxicity coefficient (CTC) is notable synergy more than 200, and 150~200 is that synergistic effect is smaller, and 70~150 is summation action, small
In 70 be antagonism (research [J] the Agricultural University Of Nanjing report of Tan Fujie, Shen Jinliang, You Zi flat insecticides mixing application,
1987,4 (increasings)：93-99).
In formula：A represents A single dose toxicity indexes；ACRepresent A shared percentage in mixture；B represents B single dose toxicity indexes；
BCRepresent B shared percentage in mixture.
Virulence of the root bark of celastrus angulatus extract with pepper extract to Wolfberry Aphid together be shown in by malicious coefficient determination result of calculation
Table 1：
The root bark of celastrus angulatus extract of table 1 and pepper extract are to the virulence of Wolfberry Aphid malicious coefficient determination result together
。
The measurement result of table 1 shows：Root bark of celastrus angulatus extract is pressed in table 1 with pepper extract two kinds of active components group
There are significant synergistic effect, root bark of celastrus angulatus extract and pepper extract 2 after ratio compounding to Wolfberry Aphid:When 1, synergy is made
With most by force, co-toxicity coefficient is 272.84；Ratio is 8:1、4:1、1:1、1:2、1:4、1:When 8, co-toxicity coefficient is followed successively by 173.43,
223.24、211.46、204.24、181.47、142.55。
9.2 pairs of Paratrioza sinica Toxicity Determinations：
The folium lycii with wood louse is taken, with the close wood louse of writing brush picking individual size, is uniformly placed on face of blade and is made
There is wood louse 20~30 on each small pieces, in the pesticide solution that blade immersion has been prepared, taken out after 5 s of dipping, with suction
Water paper sucks unnecessary liquid and dries, in the culture dish of 90 mm for being then placed in spreading the wet water absorbent paper that haves three layers, with 2 layers of wet gauze
Culture dish lid is covered after sealing, indoor temperature is maintained at (26 ± 1) DEG C.Often treatment is repeated 3 times, and is control with clear water.24 d
After investigate total borer population and dead borer population.Death standard is to touch polypide and appendage with small writing brush, and death is then considered as without any reaction（Close
Dawn celebrating.The screening of Paratrioza sinica biological agent and natural enemy Study of Sensitivity.Agriculture of Anhui science, 2009,37（10）：4541-
4542）.Calculate virulence regression equation, the lethal concentration of 50 (LC50) and co-toxicity coefficient.
Virulence of the root bark of celastrus angulatus extract with pepper extract to Paratrioza sinica together be shown in by malicious coefficient determination result of calculation
Table 2.
The root bark of celastrus angulatus extract of table 2 and pepper extract are to the virulence of Paratrioza sinica malicious coefficient determination result together
。
The measurement result of table 2 shows：Root bark of celastrus angulatus extract is pressed in table 2 with pepper extract two kinds of active components group
There are significant synergistic effect, root bark of celastrus angulatus extract and pepper extract 2 after ratio compounding to Paratrioza sinica:When 1, synergy is made
With most by force, co-toxicity coefficient is 280.34；Ratio is 8:1、4:1、1:1、1:2、1:4、1:When 8, co-toxicity coefficient is followed successively by 165.75,
221.61、206.03、197.23、172.38、132.29。
9.3 embodiment 1,3,4,6 prevents and treats Wolfberry Aphid field control effectiveness test：
Experiment is divided into two batches to be carried out, and it is first group that embodiment 1 and embodiment 4 are set respectively, and embodiment 3 and embodiment 6 are second group,
And the control sample of each embodiment is prepared respectively, the control sample of wherein embodiment 1 is that 8% root bark of celastrus angulatus extract and 1% Chinese prickly ash are carried
Thing is taken, the control sample of embodiment 3 is 8% root bark of celastrus angulatus extract and 4% pepper extract, and the control sample of embodiment 4 is 10% bitter
Skin rattan root bark extract and 5% pepper extract, the control sample of embodiment 6 is that 1.5% root bark of celastrus angulatus extract and 6% Chinese prickly ash are carried
Thing is taken, while with clear water as blank.The specific preparation method of wherein each control sample is different with reference to its corresponding each embodiment
, the active component in each embodiment is replaced with the active component of corresponding control sample, such as control sample of embodiment 1 point
Not Wei 8% root bark of celastrus angulatus extract and 1% pepper extract, the root bark of celastrus angulatus extract of control sample 8% is then by embodiment
Active component root bark of celastrus angulatus extract and pepper extract replace with root bark of celastrus angulatus extract, and other components are constant, add
Water makes active component root bark of celastrus angulatus extractive content be 8%.
Test is carried out in July, 2014 in lycium barbarum production base.3 years raw matrimony vine seedlings are selected, in Wolfberry Aphid 1st generation children
Nymph age, peak period beginning is sprayed with Backpack type mechanical mist and powder spraying machine.Often treatment is repeated 3 times, the arrangement of completely random district's groups, and sets guarantor
Shield row.50kg water/667m2, select fine day 16：After 00, the medicament that will have been configured implements complete stool spraying, emphasis sprinkling tree crown taper
And leave dual sides, accomplish uniform, careful, comprehensive covering.10 plants of trees are selected per cell, selection aphid is more in each tree 2
Individual branch is marked as fixed sample branch with label, indicates numbering, medicament title, concentration.The worm on fixed sample branch is investigated before dispenser
Mouth radix.3 d, 7 d, 15 d investigate the aphid number living that every cell is fixed on sample branch respectively from after spray（Guo Rui, Yan Lin, Jin Shengying,
Chen Sheng kingfisher DS orders Kazakhstan Wolfberry Aphid toxicity test and field control effectiveness test north gardening, 2012,（10）：148-151）.
Data analysis is carried out using SPSS22.0 softwares.The results are shown in Table 3 and table 4.
Prevention effect is to compare the data of efficacy survey with blank district, calculates prevention effect（Revision insect recluced rate）,
Computing formula is as follows：
；
。
3 first groups of embodiment preparation preventing and treating Wolfberry Aphid field control effectiveness tests of table
。
4 second groups of embodiment preparation preventing and treating Wolfberry Aphid field control effectiveness tests of table
。
The preparation of two kinds of active components group compounding of the present invention, prevents and treats the drug effect such as upper table 3 and table 4 of Wolfberry Aphid, it is known that, this
The preparation of invention two kinds of active components group compounding is significantly improved to Wolfberry Aphid prevention effect, hence it is evident that better than two kinds of active components group
It is used alone, and has preferable quick-acting.Treatment agent is the extension rate of 9%, 10% and 12% bitter 500 times of green pepper, and 3d's is anti-
Effect is 72%~97%；It is 2 in root bark of celastrus angulatus extract and pepper extract ratio：When 1（That is embodiment 3）, preventive effect is optimal,
3d, 7d, 15d after treatment, preventive effect are up to 95.23%, 98.07% and 98.06% respectively, and to target in this experiment scope of medication
It is denoted as thing and beneficial organism safety.
9.4 embodiment 1,3,4,6 prevents and treats Paratrioza sinica field control effectiveness test：
Experiment is divided into two batches to be carried out, and it is first group that embodiment 1 and embodiment 4 are set respectively, and embodiment 3 and embodiment 6 are second group,
And the control sample of each embodiment is prepared respectively, control sample is specifically prepared referring to 9.3, while with clear water as blank.Test in
In July, 2014 is carried out in lycium barbarum production base.Every kind of medicament sets up 2 concentration treatment separately, is control with clear water.Often process
3 plants of trees are chosen, random district's groups arrangement is repeated 3 times, each plant is fully sprayed up to No drip type with hand-powered knapsack sprayer
It is advisable.Check Paratrioza sinica radixes are adjusted before dispenser, respectively listing mark.The wood louse on every is investigated respectively at 3d, 5d, 7d after dispenser
Number, calculates Paratrioza sinica Revision insect recluced rate and prevention effect（Chen Peimin, Wang Jianping, clock Yangjing, Su Huiming, is several for tension force army
Medicine based on herbs preventing and treating Paratrioza sinica test of pesticide effectiveness Inner Mongol agricultural science and technology, 2013（5）： 66-67）.The results are shown in Table 5 and table 6
It is shown.
5 first groups of embodiment preparation preventing and treating Paratrioza sinica field efficacies of table
。
6 second groups of embodiment preparation preventing and treating Paratrioza sinica field efficacies of table
。
The preparation of two kinds of active components group compounding of the present invention, prevents and treats the drug effect such as upper table 5 and table 6 of Paratrioza sinica, it is known that, this
The preparation of invention two kinds of active components group compounding is significantly improved to Paratrioza sinica prevention effect, hence it is evident that better than two kinds of active components group
It is used alone, and has preferable quick-acting.Treatment agent is the extension rate of 9%, 10% and 12% bitter 500 times of green pepper, and 3d's is anti-
Effect is 70%~97%；It is 2 in root bark of celastrus angulatus extract and pepper extract ratio：When 1（That is embodiment 3）, preventive effect is optimal,
3 d, 7 d, 15 d after treatment, preventive effect are up to 96.78%, 97.73% and 98.10% respectively.
Additionally, we most significantly match 2 to root bark of celastrus angulatus extract and pepper extract synergistic effect:1 is carried out
The acute toxicity test of small white mouse, regression equation is Y=-1.13+1.61x, acute oral LD50It is 6413.23mg/kg to be worth, micro-
In malicious scope.
In sum, Pesticidal combination of the invention has strengthens Wolfberry Aphid and Paratrioza sinica prevention effect, quick-acting
Property good, lasting effect advantage long, compared with existing unitary agent, in addition to significant insecticidal effect, can effectively drop
Low dispenser cost, slows down the generation of resistance, reduces preparation residual quantity, and, security pollution-free to crop is good, meets pesticidal preparations
Security requirement.
Claims (8)
1. a kind of novel insecticidal composition, it is characterised in that its active component is root bark of celastrus angulatus extract and pepper extract.
2. novel insecticidal composition as claimed in claim 1, it is characterised in that root bark of celastrus angulatus extract and pepper extract
Weight ratio be 1~8:1~8.
3. novel insecticidal composition as claimed in claim 2, it is characterised in that the root bark of celastrus angulatus extract is by following step
It is rapid to obtain：Root bark of celastrus angulatus is crushed, root bark of celastrus angulatus powder is obtained, the ethanol of 3~5 times of root bark of celastrus angulatus powder weight is added, 60
~80 DEG C of 1~4h of ultrasonic extraction, are concentrated under reduced pressure after filtering and obtain final product；The pepper extract is obtained by following steps：By zanthoxylum powder
It is broken, the methyl alcohol of 3~6 times of zanthoxylum powder weight is added, it is concentrated under reduced pressure in 40~70 DEG C of h of ultrasonic extraction 4~6, after filtering and is obtained final product.
4. novel insecticidal composition as claimed in claim 3, it is characterised in that the Pesticidal combination is aqueous emulsion, and its is heavy
Amount percentage constitutes and is：Active component 5~12%, cyclohexanone 3~30%, Sodium Benzoate 0.5~1%, ethylene glycol 0.5~1%, synergy
Ether 1~3%, 1,2-butanediol 0.5~2%, agriculture breast 2201 are 2~5%, and remaining is water.
5. novel insecticidal composition as claimed in claim 4, it is characterised in that the percentage by weight composition of the aqueous emulsion
For：Root bark of celastrus angulatus extract 8%, pepper extract 4%, cyclohexanone 24%, Sodium Benzoate are that 1%, ethylene glycol is 1%, Butacide
3%th, 1,2-butanediol is that 0.6%, agriculture breast 2201 is 5%, and remaining is water.
6. the preparation method of any described novel insecticidal compositions of claim 1-5, it is characterised in that comprise the following steps：
1）Active component is mixed into obtain oil mixture with cyclohexanone；
2）By agriculture breast 2201, Sodium Benzoate, ethylene glycol, Butacide, 1,2-butanediol with the 1/2 of water consumption mix water is mutually mixed
Compound；
3）By step 2）The aqueous mixture for obtaining adds step 1）Mix in the oil mixture for obtaining, while supplementing surplus
Water, you can form the disinsection aqueous emulsion of oil-in-water type.
7. the preparation method of novel insecticidal composition as claimed in claim 8, it is characterised in that step 1）With step 2）In mix
That closes concretely comprises the following steps：10~30 min are stirred with the speed of 800~1000 r/min；Step 3）The specific steps of middle mixing
For：With the min of high-speed stirred 10~30 of 10000~12000 r/min.
8. the application of any described novel insecticidal compositions of claim 1-5, it is characterised in that be applied to and done harm to matrimony vine
The preventing and treating of worm.
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| CN103329943A (en) * | 2013-04-22 | 2013-10-02 | 河南省科高植物天然产物开发工程技术有限公司 | Insecticide composition containing natural celastrus angulatus and natural pyrethrin |
| CN105532763A (en) * | 2015-12-16 | 2016-05-04 | 郑晓锋 | Traditional Chinese medicine pesticide |
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| CN103329943A (en) * | 2013-04-22 | 2013-10-02 | 河南省科高植物天然产物开发工程技术有限公司 | Insecticide composition containing natural celastrus angulatus and natural pyrethrin |
| CN105532763A (en) * | 2015-12-16 | 2016-05-04 | 郑晓锋 | Traditional Chinese medicine pesticide |
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