KR100777258B1 - Acaricide using plant extract and method thereof - Google Patents

Abstract

An acaricide is provided which is harmless to human body due to be derived from a plant and promotes the acaricidal effect by mixing an acaricide promoting agent with an extract of plant with excellent acaricidal effect without inducing any tolerance. An acaricide for Tetranychus urticae or Panonychus ulmi comprises an extract of at least one plant selected from the group consisting of Atractylodes japonica, Ficus carica, and Carpesium abrotanoides as an effective ingredient. The acaricide further comprises a surfactant or at least one kind selected from the group consisting of polyoxyethylene lauryl ether, polyoxyethylene glycol, hydrotreated heavy paraffinic distillate, and sodium benzoate.

Description

Acaricide and manufacturing method using plant extract {ACARICIDE USING PLANT EXTRACT AND METHOD THEREOF}

The present invention relates to acaricide and a manufacturing method using a plant extract, sapju (Atractylodes japonica ), Fig ( Ficus) carica ), tobacco extract ( Carpesium abrotanoides ) relates to acaricides as an active ingredient extracted from.

Mite (Mite) are fruit trees, flowers, horticultural crops, including nearly all smiles pests that damage to the mite, especially damaging in apple tree spotted spider mite (Tetranychus urticae), apple mite (Panonychus ulmi ).

Spotted mite ( Tetranychus urticae ) is mainly applied on the back of the apple tree leaves, so even if the back of the leaf is discolored, the damage on the outside does not appear well. If severely damaged, the entire leaf turns yellow and leaves early. It affects not only the growth of fruit but also the amount of fruit of the following year. Occurs 8 ~ 10 times a year. After wintering from bark cracks, ground weeds and deciduous leaves to adults, it grows mainly in the ground weeds around April and May. do. In the early stages, it begins to penetrate from the inside of the water department, especially near the early opening eyes of the daytime, and when the density increases, it spreads out of the crown. It reaches the highest density in August-September, and wintering adults start to appear in late September, moving to the coriander along the daytime, or falling to the ground with fallen leaves.

Panonychus ulmi ) is absorbed from the front and the back of the leaf, so the damaged leaf becomes yellowish brown, deteriorating the function of the leaf, severely affects the hypertrophy of the fruit, pigmentation, flower bud formation. Occurs seven to eight times a year and overwinters in the eggplant branch or winter snow donations, so the spawning area is red when there are many eggs. Overwintered eggs hatch from the late April to early May, when the apple tree blooms, adding young leaves around the flowers. After 2 to 3 weeks, the eggs are grown. The eggs are laid in the leaf vein around the leaf veins. From the third generation, each developmental condition is mixed, and the leaf sugar density decreases temporarily by dispersing into young leaves from late May to mid June, but increases rapidly during the summer multiplication period after late June, usually from late July to late August To reach the highest density. There are females that produce winter eggs from late September, and they spawn a lot after mid-October, and if the leaves are in good condition, they may continue to spawn on branches even when it snows, but when the leaves are prematurely damaged, the spawning season will accelerate. .

In order to control such mites, the use of chemical pesticides is the mainstream. Currently, these control agents include fluacrypyrim-tetradifon liquid hydrate (fluxrypyrim-tetradifon, 2000 times), spirodiclofen hydrate (spirodiclofen, 2000 times), milbemectin emulsion (1000 times milbemectin), propargite (propargite). , 1000 times), benzome emulsion (benzoximate, 1000 times), bifenazate liquid hydrate (bifenazate, 2000 times), hexahexatin hydrate (cyhexatin, 1500 times), acequinocyl hydrate (acequinocyl, 1000 times) , Azocyclotin (1,500 times), azocyclotin liquid, (azocyclotin, 2000 times), ethoxazole liquid (0x, 4000 times), chiax hydrating (hexythiazox, 2000 times), tebufenpyrad emulsion (tebufenpyrad) , 2000 times), phenazaquin liquid hydrating agent (fenazaquin, 3000 times), pentbutane emulsion (fenbutatin oxide, 1000 times), fenpyroximate liquid hydrating agent (fenpyroximate, 2000 times), pyridaben-azocyclotin hydration (pyridaben-azocyclotin) , 1000 times), pyrimidife-bifenthrin hydration (pyrimidife Many drugs, such as 1000 times n-bifenthrin) have been announced. This is known to inhibit growth such as hatching, lethality, egg laying inhibition and infertility throughout all stages of growth such as eggs, larvae, and adults. However, in the early stage, the effect is excellent, about 80 ~ 90%, but due to the resistance to the drug, the control value gradually decreases to 50 ~ 60%, which is very insufficient. I'm doing it.

Recently, a number of biological control methods using antagonists and various plant extracts have been studied, and these methods do not cause resistance in addition to the advantages of not causing problems such as environmental pollution, weakness, residual toxicity, etc. It is considered to be a very good control method.

Biomaterials that can control pests include insecticidal microorganisms, insecticidal plant extracts, and insecticidal oils. Insecticidal microorganisms include the genus Verticillium , Metarhizium , Paecilomyces , Beauveria , and Hirsutrlla , and many studies have been conducted at home and abroad, but until now, the effectiveness of the control is not high. Insecticidal plants such as red ginseng, Astragalus, ginkgo biloba, tobacco leaves, etc. are recognized to have a pesticidal effect on micropests such as mites, but this also does not show satisfactory control value. In addition, insecticide oils include Nime Oil, Jojoba Oil, Canola Oil, and Carnolar Oil, which have some insecticidal properties, but the effect is too low to be used as a pest control agent. have. The same is true for other useful ingredients known to be pesticidal.

Biopesticides developed using plant components are relatively weak, and representative pesticides using plant extracts are commercialized such as chrysanthemum flowers, natural pyrethrin extracted from plants, nicotine extracted from tobacco, and insecticides using gosam root. Many other biopesticides have been developed, but their types are not diverse. In addition, there is a lack of formulation technology capable of properly exerting the effect of formulating the plant extract and other mixed ingredients, the effect is weak compared to chemical pesticides or synthetic pesticides.

In addition, in order to improve the income of farmers and secure stable profitability, it is necessary to develop technologies that can produce crops in an environmentally friendly manner. In the case of crop cultivation, especially pesticides are used, in order to produce eco-friendly crops, bio pesticides for pest control should be developed first. Among pests in question, moths can be controlled to some extent using B. T., but in the case of micropests such as mites, there are few effective eco-friendly control agents. In the case of mites, the generation period is short and the breeding speed is very high, so that the resistance to chemical pesticides is severely induced, and the continuous spraying effect lowers the control effect. Therefore, in the present invention, to develop a biopesticide that can control the mites, among the various micro-pest, to reduce the high rise of the farmers to grow eco-friendly crops.

An object of the present invention is to solve the above problems, to find a plant extract with excellent acaricide effect from the plant and at the same time to mix the acaricide enhancer to further enhance the acaricide effect, for adjusting the physical properties, stabilizers, etc. The development of acaricides in combination with no induction of resistance, harmless to humans to provide plant-derived acaricides.

Hereinafter, the present invention will be described in detail.

In the present invention, 'acaricide' means a drug that selectively kills mites.

In the present invention, the 'extract' is a material extracted from a plant, and may be liquid, concentrate, mixed liquid or solid, or powder, and is used as a concept including a crude extract. In addition, the concept includes a further refined crude extract.

In the present invention, the 'plant extract' may be used to refer to the extract extracted from the 'injection', 'figure', 'cigarette' of the present invention, it may be used as a mixture thereof.

In the present invention, the 'surfactant' may mean the same as the 'surfactant' and is a concept including a nonionic surfactant.

In the present invention, '%' of the compounding ratio means 'volume%' unless stated otherwise.

The present inventors screened 100 species of plants expected to have an insecticidal effect against various pests through various literatures, and extracted them with an organic solvent to obtain crude extarct, and then inoculated them in a mite and screened them firstly for 32 species. The plant extracts having excellent acaricide effect were selected, and again, through the second screening, the insert, fig, and tobacco grass extract confirmed that the acaricide effect was very excellent to complete the present invention. In addition, each of the three plant extracts were mixed with each other to test the killing effect. Here, the killing effect is to have a killing power for the mite, in the present invention spotted mite ( Tetranychus urticae ) and apple mite ( Panonychus ulmi ) was used to measure the acaricide effect.

The present invention provides acaricides comprising an extract extracted from one or more plants selected from the group consisting of shovels, figs and tobacco grass. The extract includes crude extract. In addition, the extract is generally liquid, and may be concentrated or diluted. The injectable extract, fig extract, tobacco extract can be used in a certain ratio mixed with each other, each extract exhibits an excellent acaricide effect, the mixing ratio is not limited. However, it is more preferable that they are mixed in equal proportions for the optimum killing effect.

The extraction of the plant is preferably extracted with a lower alcohol of C1 ~ C4, more preferably with methanol. The extraction temperature is not limited but is about 50 ° C., and the extraction time is not limited but preferably about 24 hours or more.

The application of the acaricide is not limited to a specific mite, but the spotted mite ( Tetranychus urticae ) or apple mite ( Panonychus ulmi ) is preferred.

The present invention also provides acaricides further comprising a surfactant as acaricide enhancer to increase the mite contact efficiency of the plant extract.

The surface active agent is not particularly limited, but is preferably polyoxyethylene tridecyl ether, Sophorolipid, or a mixture thereof.

The present invention also provides acaricides further comprising a physical property modifier or stabilizer to stabilize the acaricide active ingredient of the plant extract.

The physical property modifier is not particularly limited, but is preferably selected from polyoxyethylene lauryl ether, polyoxyethylene glycol, and hydrotreated heavy paraffinic distillate. .

The stabilizer is sodium benzoate, potassium sorbate, sodium propionate, and sodium benzoate is more preferred.

The plant extract may be used alone as an acaricide, and when used in combination with a surface active agent, a physical property modifier or a stabilizer as a potentiator, it is preferably about 50 to 90% of the total acaricide, and the surface active agent is 3 to 25% It is preferable that it is preferable that a physical property modifier is 3 to 25%, and a small amount of stabilizer may be sufficient. The crude extract content of the present invention is preferably applied differently depending on the formulation of the acaricide, spraying method. The formulation of the mite acaricide in the present invention is in the liquid phase and the spraying method can be dilute in water sufficiently to be treated with foliage.

Example  1: from 100 plant species Crude extract  Produce

Through various literatures, 100 kinds of plants of Table 1, which are expected to have pesticidal effects against various pests, were selected, and then, each of them was pulverized finely in a blender, 200 g each, put in a glass bottle, and then mixed with 1000 ml of methanol at 24 ° C. at 24 ° C. It was left for time. After 24 hours, the mixture was filtered and the extract solution was concentrated using a rotary vacuum concentrator (EYELA N-1000, Japan). The concentrate was further mixed with methanol to prepare a crude methanol extract.

Botanical name Scientific name Botanical name Scientific name Leather tree Ailanthus altissima Shovel Atractylodes japonica Rooting Puerariae radix Ginger Tree Lindera obtusiloba Brown oak Quercus aliena Pine tree Pinus densiflora Forget-me-not Erigeron annuus Hawthorn Carpinus coreana Hibiscus Cephalotaxus koreana Flower star Stellaria aquatica Personalization Lycoris aurea purslane Portulaca oleracea A defector Cassiae semen Mugwort Artemisia princeps  var. orientalis Bracken Pteridium aquilinum  var. latiusculum Way Veratrum maackii Gosam Sophora flavescens Fellowship Forsythiae fructus Chilli Staphylea bumalda Schisandra Schisandra chinensis crowd Dryopteris crassirhizoma burdock Arctium lappa Hoebab Oxalis corniculata A lieutenant ship Clematidis radix Copper Angelica dahurica nutmeg Myristicae semen Oyster tree Platycarya strobilacea Ginkgo Ginkgo biloba Beech Meliosma myriantha Indong Lonicera japonica Tree Clerodendrum trichotomum Injin Artemisiae lwayomogii herba Elm tree Ulmus davidiana var . japonica Jawan Asteris radix Salvia Salviae radix Weasel Amorpha fruticosa Tobacco paste Carpesium abrotanoides Mothball Vitex negundo var . incisa Ivy Parthenocissua tricuspidata Hawthorn Adina rubella Dang Vine Cocculus trilobus Fund Ardisia japonica Pheasant tree Viburnum erosum grass Zoysia japonica Dead wood Styrax japonica War Anthriscus sylvestris Camellia Camellia japonica Cooperative Gleditsiae fructus Arbor Aralia elata True Aconitum pseudolaeve  var. erectum Perilla Mosla punctulata Straw Sprouts Agrimonia pilosa Buckthorn Fraxinus mandshurica Participating tree Quercus salicina knotgrass Polygonum aviculare A true tree Euonymus sieboldiana Mansu national treasure Tagetes minuta Pendulum Euonymus oxyphyllus sulphate of soda Erigeron canadensis calamus Acorus calamus var . angustatus Mulberry Melia azedarach var . japonica Cheonnam Province Arisaema amurense buckwheat Fagopyrum esculentum Cheongmirae vine Smilax china Persimmon tree Koelreuteria paniculata Dogwood Cornus controversa Neck skin Moutan cortex radicis Bark tree Zanthoxylum piperitum elecampane Saussuteae radix Civil incense Inulae radix FIG Ficus carica Tobok Spirit Smilacis rhizoma dandelion Taraxacum mongolicum Pagokcheon Stream Morindae radix wire grass Digitaria sanguinalis Big swallow Delphinium maackianum Chick Veratrum patulum Fly Phryma leptostachya var . asiatica mint Mentha arvensis var . piperascens White Chamaecyparis obtusa White Bletillae rhizoma Palma Christi Ricinus communis thyme Thymus quinque costatus Hagocho Prunellae spica Circumference Stemonae radix Nasturtium Eclipta prostrata Barley Rice Elaeagnus macrophylla Hyangbuja Cyperi rhizoma Bokbunja Strawberry Rhus coreanus Corydalis Corydalis turtschaninovii Rhododendron Rhus chinensis Gold Scutellariae radix Tree Lindera erythrocarpa Astragalus Astragali radix Non-tree Torreya nucifera Widower Chloranthus japonicus Mountain bark Rhus sylvestris Thick Magnoliae cortex Japanese tree Zanthoxylum schinifolium A painting tree Sophora japonica

Experimental Example  One: Acaricide effect  Primary screening

A. Acaricide effect  Mites used for black

Acaricide activity is spotted mite ( Tetranychus urticae ). Spotted mite was grown using kidney beans, photoperiod at 16: 8 (L: D) h, temperature at 25 ° C, and humidity at 75%. Mite-proliferating leaves were inoculated into new host leaves at intervals of 3-4 days.

B. Acaricide effect  black

A circular bean leaf having a diameter of 3 cm was immersed in the plant extract treatment solution of Example 1 (all 2,000 ppm) for about 5 minutes and then dried. The dried soybean leaves were transferred to a 6 cm diameter culture vessel with wet filter paper (3.5 cm in diameter). 40 adults were inoculated and transferred back to the mite propagation incubator. After 1, 3 and 5 days, the effects of acaricide on the mites were analyzed. The lethal mites were judged when there was no arbitrary movement of the stimulus of the insect pins.

Table 2 below shows the results of analyzing the acaricidal effect on spotted M. larvae of 100 methanol crude extracts. The killed number of spotted mites was analyzed for a total of 5 days after treatment, and the screening criteria for fast-acting screening were extracts showing more than 85% of acaricide on day 3.

100 kinds of methanol for spotted mites Crude extract (2000 ppm 1st screening result Plant name Live animals Killed water after treatment with 2000ppm methanol crude extract 1 day later 3 days later 5 days later Leather tree 40 0 11 19 Rooting 40 0 0 3 Brown oak 40 0 40 40 Forget-me-not 40 0 10 32 Hibiscus 40 0 30 40 Personalization 40 0 40 40 A defector 40 0 2 17 Bracken 40 0 0 8 Gosam 40 0 19 34 Chilli 40 0 6 21 crowd 40 0 30 40 Hoebab 40 0 33 40 Copper 40 0 0 3 Oyster tree 40 0 0 5 Beech 40 0 40 40 Tree 40 0 15 31 Elm tree 40 0 35 40 Salvia 40 0 30 40 Tobacco paste 40 0 38 40 Ivy 40 0 0 20 Dang Vine 40 0 40 40 Pheasant tree 40 0 30 40 Dead wood 40 0 25 32 Camellia 40 0 13 22 Arbor 40 0 28 34 Perilla 40 0 34 40 Buckthorn 40 0 26 32 knotgrass 40 0 20 24 Mansu national treasure 40 0 13 25 sulphate of soda 40 0 0 7 Mulberry 40 0 21 28 buckwheat 40 0 35 40 Persimmon tree 40 0 20 27 Neck skin 40 0 18 20 elecampane 40 0 32 38 FIG 40 0 37 40 dandelion 40 0 34 40 wire grass 40 0 20 27 Chick 40 0 0 9 mint 40 0 14 22 White 40 0 22 28 thyme 40 0 25 35 Circumference 40 0 40 40 Barley Rice 40 0 40 40 Bokbunja Strawberry 40 0 24 33 Rhododendron 40 0 40 40 Tree 40 0 40 40 Non-tree 40 0 30 40 Mountain bark 40 0 35 40 Japanese tree 40 0 36 40 Shovel 40 0 37 38 Ginger Tree 40 0 40 40 Pine tree 40 0 0 6 Hawthorn 40 0 0 3 Flower star 40 0 35 40 purslane 40 0 0 18 Mugwort 40 0 40 40 Way 40 0 34 40 Fellowship 40 0 19 29 Schisandra 40 0 36 40 burdock 40 0 32 38 A lieutenant ship 40 0 0 13 nutmeg 40 0 0 4 Ginkgo 40 0 40 40 Indong 40 0 35 40 Injin 40 0 16 31 Jawan 40 0 0 3 Weasel 40 0 14 20 Mothball 40 0 38 40 Hawthorn 40 0 13 26 Fund 40 0 15 27 grass 40 0 25 28 War 40 0 34 40 Cooperative 40 0 28 40 True 40 0 16 28 Straw Sprouts 40 0 27 35 Participating tree 40 0 34 40 A true tree 40 0 0 9 Pendulum 40 0 16 23 calamus 40 0 36 40 Cheonnam Province 40 0 0 13 Cheongmirae vine 40 0 40 40 Dogwood 40 0 40 40 Bark tree 40 0 16 26 Civil incense 40 0 17 24 Tobok Spirit 40 0 0 17 Pagokcheon Stream 40 0 16 21 Big swallow 40 0 26 36 Fly 40 0 34 40 White 40 0 12 26 Palma Christi 40 0 0 13 Hagocho 40 0 9 26 Nasturtium 40 0 37 40 Hyangbuja 40 0 0 18 Corydalis 40 0 14 26 Gold 40 0 15 30 Astragalus 40 0 8 24 Widower 40 0 18 26 Thick 40 0 17 23 A painting tree 40 0 10 23

As shown in Table 2, as a result of the primary screening at 2,000 ppm, 32 plant extracts (bold letters) showed excellent acaricide effect. However, their fertilizing power was obtained at a relatively high concentration, and in order to apply to the product, the extracts showing the fertilizing effect at a relatively low concentration were selected again.

Experimental method was the same as the primary screening method, but the crude extract concentration was reduced, and the soybean leaf having a diameter of 3 cm was immersed in methanol crude extract treatment solution (all 1,000 ppm) for about 5 minutes, and dried. The dried soybean leaves were transferred to a 6 cm diameter culture vessel with wet filter paper (3.5 cm in diameter). 60 adults were inoculated and transferred back to the mite propagation incubator. After 2, 3, 4 and 5 days, the acaricide effect on the treated mites was analyzed. The lethal mites were judged when there was no arbitrary movement of the stimulus of the insect pins.

Table 3 below shows the results of analyzing the fertilization effect on the spotted mite adult extract of 32 kinds of crude methanol extracts selected through primary screening. The killed number of spotted mites was analyzed for a total of 5 days after treatment, and the screening criterion was selected on the 5th day of the extract showing over 80% of acaricide.

32 kinds of methanol for spotted mites Crude extract (1000 ppm ) Second screening result Plant name Live animals Killed water after 1000ppm of methanol crude extract 2 days later 3 days later 4 days later 5 days later Brown oak 60 3 10 18 28 Personalization 60 5 21 31 43 Beech 60 3 3 5 10 Elm tree 60 3 13 29 30 Tobacco paste 60 29 36 43 49 Dang Vine 60 2 2 3 3 Perilla 60 0 5 9 9 buckwheat 60 0 One 2 2 FIG 60 22 32 50 56 dandelion 60 4 20 28 31 Circumference 60 2 9 15 18 Barley Rice 60 2 3 16 33 Rhododendron 60 0 5 10 12 Tree 60 4 6 9 19 Mountain bark 60 0 2 11 28 Japanese tree 60 0 One 6 9 Shovel 60 19 42 56 59 Ginger Tree 60 4 22 31 44 Flower star 60 6 15 20 20 Mugwort 60 3 5 14 27 Way 60 3 4 5 8 Schisandra 60 0 3 10 12 Ginkgo 60 3 7 8 10 Indong 60 0 3 4 6 Mothball 60 0 One 2 5 War 60 4 8 11 19 Participating tree 60 One 5 11 12 calamus 60 2 8 14 17 Cheongmirae vine 60 3 8 24 27 Dogwood 60 0 One 8 15 Fly 60 4 9 10 13 Nasturtium 60 One 4 8 16

As shown in Table 3, the secondary screening results showed that the infusions, figs, and tobacco extracts had a high killing power of 80% or more after 5 days of treatment even at a concentration of 1,000 ppm.

Experimental Example  2: Shovel , Fig, according to Tobacco Extract Extract Acaricide effect  compare

In order to more effectively enhance the acaricide effect of the inserts, figs, tobacco grass extract selected in Table 3 above, we compared the fertilization effect when using the plant extract alone and a mixture of plant extracts in a certain ratio. In this experiment, we compared the fertilizing effect by mixing each plant extract in a 1: 1 ratio against spotted mites.

A circular bean leaf having a diameter of 3 cm was immersed in each methanol crude extract treatment solution (1,000 ppm) for about 5 minutes and then dried. The dried soybean leaves were transferred to a 6 cm diameter culture vessel with wet filter paper (3.5 cm in diameter). 60 adults were inoculated and transferred back to the mite propagation incubator. After 2, 3, 4 and 5 days, the fertilizing effect on the treated mites was analyzed. The lethal mites were judged when there was no arbitrary movement of the stimulus of the insect pins.

Shovel , Fig, according to Tobacco Extract Extract Acaricide effect  compare Experiment Live animals Killed water after 1000ppm of methanol crude extract 2 days later 3 days later 4 days later 5 days later Tobacco paste 60 28 34 42 48 FIG 60 25 35 48 56 Shovel 60 27 40 50 56 Tobacco paste + fig 60 27 35 47 53 Tobacco paste + shovel 60 26 36 45 54 Fig + Insert 60 30 40 52 57 Tobacco Paste + Fig + Shovel 60 33 42 53 59

As shown in Table 4, the tobacco paste, the figs, and the inserts were treated at a ratio of 1: 1: 1, respectively.

Example  2: containing more surfactant Acaricide  Produce

In order to contact the tobacco grass, fig, and the shochu extract mixture (tobacco grass: figur: injection = 1: 1: 1, 1000ppm) selected in Table 4 above to the mites, a surfactant was added as a fertilizer.

Surface active agents include polyoxyethylene oleyl ester-9, polyoxyethylene nonylphenyl ether-20, sophorolipid, polyoxyethylene castor ether- 15, polyoxyethylene tridecyl ether-7, polyoxyethylene sorbitan fatty acid ester-5, polyoxyethylene hydrogenated castor ether-15 10, 30, 50, 70, 100 ppm were mixed into the extract mixture.

Experimental Example  3: more surfactant is included Acaricide  Effect black

The effect of the acaricide prepared in Example 2 was assayed.

The fertilization effect assay was carried out by spraying method, and the insect pest, Tetranychus ulticae ) was grown using kidney beans. Photoperiod was 16: 8 (L: D) h, temperature was grown at 25 ℃, 75% humidity conditions, and mite-proliferating leaves were inoculated into new host leaves at intervals of 2 to 3 days to prevent overdensity. . The mites grown on kidney beans were distributed based on 60-100 eggs per leaf for fairness.

Concentrations of Plant Extracts and Surfactants for Spotted Mite Acaricide effect Sample Name Surfactant Concentration (ppm) Live animals Killed water after drug treatment Control is (%) 2 days 3 days Plant Extract Mixture - 86 48 63 73.3 Plant Extract Mixture + Polyoxyethylene oleyl ester-9 10 94 54 70 74.5 30 83 48 63 75.9 50 88 51 67 76.1 70 85 49 64 75.3 100 76 44 57 75.0 Plant extract mixture + Polyoxyethylene nonylphenyl ether-20 10 90 52 67 74.4 30 78 45 59 75.6 50 67 39 52 77.6 70 98 56 76 77.6 100 86 49 67 77.9 Plant extract mixture + Sophorolipid 10 76 46 58 76.3 30 63 42 52 82.5 50 83 56 72 86.7 70 84 57 73 86.9 100 96 66 83 86.5 Plant extract mixture + Polyoxyethylene castor ether-15 10 95 56 72 75.8 30 91 53 70 76.9 50 84 49 65 77.4 70 79 47 62 78.5 100 63 38 50 79.4 Plant Extract Mixture + Polyoxyethylene tridecyl ether -7 10 62 40 50 80.6 30 78 53 67 85.9 50 85 60 75 88.2 70 97 67 86 88.7 100 93 65 83 89.2 Plant extract mixture + Polyoxyethylene sorbitan fatty acid ester-5 10 82 48 61 74.4 30 87 51 66 75.9 50 74 44 56 75.7 70 92 56 70 76.1 100 81 50 63 77.8 Plant Extract Blend + Polyoxyethylene hydrogenated castor ether-15 10 65 37 48 73.8 30 72 41 53 73.6 50 76 42 56 73.7 70 82 46 61 74.4 100 78 45 58 74.4

As shown in Table 5, as a result of evaluating the potentiation effect by mixing a variety of surface active agents by concentration, the potentiation effect is further enhanced in Sophorolipid (Polyoxyethylene tridecyl ether) -7 It could be confirmed. At 50 ppm, the 3rd day of fertilization increased by 13.4% and 14.9%, respectively. Such surfactants have properties that allow plant extracts to effectively adsorb to the surface of pests and to effectively penetrate into the surface of pests. In general, the synergistic effect was increased at 70ppm and 100ppm with increasing concentration.

Example  3: more stabilizer and physical property adjustment agent Acaricide  Produce( Formulation  Selection of additives)

In order to formulate the acaricidal active ingredient and the effect-promoting additive (surfactant) of the present invention, the stability and storage stability of the active ingredient are required.

Thus, the extract mixture of Example 2 (tobacco: fig: injection = 1: 1: 1), a mixture of Sophorolipid, Polyoxyethylene tridecyl ether-7, as a stabilizer and a physical property modifier. Various nonionic surfactants were mixed at different concentrations to observe physical property changes and to select formulation additives.

As a result, as physical property modifier, polyoxyethylene lauryl ether-5 2%, polyoxyethylene glycol-400 3%, hydrotreated heavy paraffinic distillate 2% or more When mixed, there was no precipitation of the active ingredient, separation of layers, and the like.

As a stabilizer, sodium benzoate, potassium sorbate, and sodium propionate were treated by concentration, so that 0.5% of sodium benzoate was mixed without change in physical properties. It appeared to show stable physical properties.

Example  4

Tobacco paste, fig, injectable extract mixture (tobacco paste: fig: inject = 1: 1: 1, 1000ppm) 75%, polyoxyethylene tridecyl ether-7 5%, phosphorelipid 5%, physical property modifier 9.5% (poly Acaricide was prepared by mixing 2% oxyethylene lauryl ether-5, 3% polyoxyethylene glycol-400, 4.5% hydrotreated heavy paraffin distillate), 0.5% sodium benzoate and 5% ethanol.

Example  5

Tobacco paste, fig, injectable extract mixture (tobacco paste: fig: inject = 1: 1: 1, 1000ppm) 75%, polyoxyethylene tridecyl ether-7 4%, phosphorelipid 4%, physical property modifier 7% (poly Acaricide was prepared by mixing 2% oxyethylene lauryl ether-5, 3% polyoxyethylene glycol-400, 2% hydrotreated heavy paraffin distillate), 0.5% sodium benzoate and 9.5% ethanol.

Example  6

Tobacco paste, fig, injectable extract mixture (tobacco paste: fig: inject = 1: 1: 1, 1000ppm) 80%, polyoxyethylene tridecyl ether-7 5%, phosphorelipid 5%, physical property modifier 9.5% (poly Acaricide was prepared by mixing 2% oxyethylene lauryl ether-5, 3% polyoxyethylene glycol-400, 4.5% hydrotreated heavy paraffin distillate) and 0.5% sodium benzoate.

Example  7

Tobacco paste, fig, injectable extract mixture (tobacco paste: fig: inject = 1: 1: 1, 1000ppm) 80%, polyoxyethylene tridecyl ether-7 4%, phosphorelipid 4%, physical property modifier 7% (poly Acaricide was prepared by mixing 2% oxyethylene lauryl ether-5, 3% polyoxyethylene glycol-400, 2% hydrotreated heavy paraffin distillate), 0.5% sodium benzoate and 4.5% ethanol.

Example  8

Agaricide was prepared by mixing 75% tobacco and 25% ethanol extract, tobacco extract, injectable extract mixture (tobacco: figur: injection = 1: 1: 1, 1000ppm).

Example  9

Agaricide was prepared by mixing tobacco extract, fig, and shovel extract mixture (tobacco paste: fig: shovel = 1: 1: 1, 1000 ppm) 80% and ethanol 20%.

Experimental Example  4: Acaricide  Of composition Acaricide effect  exam

The acaricide effect assay for the acaricides prepared in Examples 4 to 7 was carried out by a spray method, and the control (Examples 8 and 9) was compared with two of the extract mixture 75% and 80% except the additive. Spotted mite ( Tetranychus) which is target pest ulticae ) was grown using kidney beans, photoperiod was 16: 8 (L: D) h, temperature was grown at 25 ℃, 75% humidity conditions. In addition, mite-proliferating leaves were inoculated into new host leaves at intervals of 2 to 3 days to prevent overdense. The mites grown on kidney beans were distributed based on 60-100 eggs per leaf for fairness.

The treatment concentration of the prototype was diluted 500 times, 1000 times, and 2000 times to spray the kidney beans, and the spraying amount was sprayed to the extent that the leaves were sufficiently wet.

The acaricide effect on the treated mites after 2 and 3 days was analyzed. The lethal mites were judged when there was no arbitrary movement of the stimulus of the insect pins. Each test was carried out with three repeats, and a total of three times were tested for acaricide effect.

Example  4 to 9 Acaricide  Compare Effect Dilution factor Live animals Killed water after drug treatment Control price (%) Average 2 days 3 days Example 4 500 times 1 repetition 93 68 79 84.9 85.1 2 repetitions 86 65 73 84.9 3 repetitions 82 61 70 85.4 1000 times 1 repetition 78 46 57 73.1 73.1 2 repetitions 80 47 58 72.5 3 repetitions 65 38 48 73.8 2000 times 1 repetition 77 40 49 63.6 63.5 2 repetitions 72 37 46 63.9 3 repetitions 84 44 53 63.1 Example 5 500 times 1 repetition 83 58 69 83.1 83.2 2 repetitions 86 61 71 82.6 3 repetitions 81 57 68 84.0 1000 times 1 repetition 95 54 68 71.6 71.6 2 repetitions 78 44 56 71.8 3 repetitions 84 48 60 71.4 2000 times 1 repetition 90 46 56 62.2 62.4 2 repetitions 87 45 54 62.1 3 repetitions 89 46 56 62.9 Example  6 500 times 1 repetition 75 61 69 92.0 92.7 2 repetitions 87 71 81 93.1 3 repetitions 84 68 78 92.9 1000 times 1 repetition 79 58 64 81.0 80.1 2 repetitions 91 66 72 79.1 3 repetitions 86 62 69 80.2 2000 times 1 repetition 83 47 57 68.7 68.7 2 repetitions 85 48 58 68.2 3 repetitions 88 51 61 69.3 Example 7 500 times 1 repetition 90 68 77 85.6 86.2 2 repetitions 92 69 79 85.9 3 repetitions 77 59 67 87.0 1000 times 1 repetition 97 64 74 76.3 76.0 2 repetitions 81 53 62 76.5 3 repetitions 85 55 64 75.3 2000 times 1 repetition 87 46 58 66.7 67.7 2 repetitions 68 37 46 67.6 3 repetitions 74 39 51 68.9 Example 8 500 times 1 repetition 91 55 71 78.0 77.6 2 repetitions 72 43 56 77.8 3 repetitions 78 46 60 76.9 1000 times 1 repetition 84 48 59 70.2 70.4 2 repetitions 87 49 61 70.1 3 repetitions 69 39 49 71.0 2000 times 1 repetition 83 42 52 62.7 61.9 2 repetitions 93 47 57 61.3 3 repetitions 81 42 50 61.7

Example 9 500 times 1 repetition 76 47 61 80.3 81.0 2 repetitions 88 55 72 81.8 3 repetitions 89 54 72 80.9 1000 times 1 repetition 68 40 49 72.1 72.1 2 repetitions 80 47 58 72.5 3 repetitions 85 50 61 71.8 2000 times 1 repetition 82 42 52 63.4 63.0 2 repetitions 93 48 58 62.4 3 repetitions 90 47 57 63.3

As shown in Table 6, in the composition of Example 6, the astringent effect on the spotted mite was shown to be the best compared to Examples 8 and 9, which is a control, and showed a more than 90% acaricide effect when sprayed 500 times. It was.

example Acaricide of the invention  In packaging Acaricide effect  exam

Experimental Example  5: Gyeongbuk Andong Field Experiment

The acaricides prepared in Example 6 were tested for acaricide effect in the packaging of Andong, Gyeongbuk. Packaging (apple tree) salbi effect test was 500 times, the process 1000 times the foliage in T. urticae and apples mites are generators, PET support is a control ^TM in (fluorenyl arc repeater rim -tetrahydro diphone liquid wettable powder, fluacrypyrim-tetradifon) and Cinawi ^TM (spirodiclofen hydrous, spirodiclofen) was compared. Pavement management was conducted in accordance with the general farming method of farmhouses, and it was carried out with 4 repetitions of 4 weeks per test zone. The survey method was to examine the number of viable insects of 40 leaves per test, and a total of five surveys.

Andong, Gyeongbuk Slow  Pavement Experiment Results Test drug Density before treatment (average per bird) Live animals Control is (%) 1 repetition 2 repetitions 3 repetitions Average Plant Extract (500 times) 302.3 9.3 18.9 20.8 16.3 92.4 Plant Extract (1000 times) 263.7 34.5 31.9 42.5 36.3 83.0 Zippet (1500 times) 288.0 10.4 9.4 5.2 8.3 96.1 Sinawi (3000x) 214.0 10.3 50.9 31.3 30.8 85.7 No treatment 268.3 216.2 164.4 264.6 215.1 -

As shown in Table 7, the plant extract showed a control value of 92.4% at 500 times, and a control value of 83% at 1000 times. The control price was 3.5% weaker than the control drug Gifett, but it was 6.7% better than Sinawi.

Experimental Example  6: Gyeongbuk Uiseong-gun packing experiment

The acaricides prepared in Example 6 were tested for the acaricide effect in Jusangni-ri, Gyeongsangbuk-do, Korea. A salbi effect black in the packaging (apple tree) was 500 times in T. urticae and apples mites the generator, the process 1000 times the foliage, contrast agents Perot arm ^TM (ASSI tin wettable powder, azocyclotin) and Boramae ^TM (Pena Zakynthos liquid wettable powder , fenazaquin) were compared. Pavement management was conducted in accordance with the general farming method of farmhouses, and it was carried out with 4 repetitions of 4 weeks per test zone. The survey method was to examine the number of viable insects of 40 leaves per test, and a total of five surveys.

Uiseong-gun, Gyeongbuk Single sided  Juseon-ri packing experiment result Test drug Density before treatment (average per bird) Live animals Control is (%) 1 repetition 2 repetitions 3 repetitions Average Plant Extract (500 times) 296.5 19.6 17.8 9.5 15.6 93.2 Plant Extract (1000 times) 268.7 34.4 35.5 43.7 37.9 84.1 Faroepal (1500 times) 285.3 9.4 15.2 11.6 12.1 94.3 Boramae (3000 times) 274.0 21.2 37.8 29.6 29.5 87.6 No treatment 249.7 236.2 216.4 234.5 229.0 -

As shown in Table 8, the plant extract showed a control value of 93.2% at 500 times, and a control value of 84.1% at 1000 times. The control price is 1.1% lower than the control drug Ferropal, but the control price is 5.6% better than that of Boramae.

Experimental Example  7: Chungnam Dangjin-gun packing experiment

The acaricides prepared in Example 6 were tested for the acaricide effect in Seokwoori Pavement in Hapdeok-eup, Dangjin-gun, Chungbuk. A salbi effect black in the packaging (apple tree) was 500 times in T. urticae and apples mites the generator, the process 1000 times the foliage, contrast agents Perot arm ^TM (ASSI tin wettable powder, azocyclotin) and Boramae ^TM (Pena Zakynthos liquid wettable powder , fenazaquin) were compared. Pavement management was conducted in accordance with the general farming method of farmhouses, and it was carried out with 4 repetitions of 4 weeks per test zone. The survey method was to examine the number of viable insects of 40 leaves per test, and a total of five surveys.

Experimental Results of Seokuri Pavement in Hapdeok-eup, Dangjin-gun, Chungnam Test drug Density before treatment (average per bird) Live animals Control is (%) 1 repetition 2 repetitions 3 repetitions Average Plant Extract (500 times) 283.6 13.9 18.7 16.6 16.4 92.8% Plant Extract (1000 times) 227.4 29.1 37.2 40.5 35.6 82.7% Faroepal (1500 times) 282.5 19.6 16.3 15.9 17.3 92.4% Boramae (3000 times) 245.2 28.5 26.8 33.4 29.6 86.5% No treatment 251.0 226.4 228.7 194.5 216.5 -

As shown in Table 9, the plant extract showed a control value of 92.8% at 500 times, and a control value of 82.7% at 1000 times. The control price was 0.4% better than that of the control drug Ferropal, and 6.3% better than Boramae.

As shown in Tables 7 to 9, the packaging test of the acaricides prepared in Example 6 showed 92.4%, 93.2%, and 92.8% acaricide effect at 500 times. Therefore, compared to the chemical pesticides used as a control, Jipet, Sinawi, Ferropal, Boramae was confirmed to have excellent or comparable potency.

As described above, the acaricide using the injectable, fig, and tobacco grass extracts of the present invention has an excellent control effect against spotted mite and apple mite, and has an excellent or comparable control effect compared with conventionally disclosed chemical pesticides.

Claims (7)

Acaricides containing an extract extracted from one or more plants selected from the group consisting of shovels, figs, and tobacco grass as an active ingredient. The acaricide according to claim 1, wherein the extract is extracted with C1 ~ C4 lower alcohols. The method according to claim 1 or 2, wherein the acaricide is Tetranychus urticae or apple mite ( Ponyonychus) acaricide that is to anoint ulmi ). The acaricide according to claim 1 or 2, further comprising a surfactant. The acaricide according to claim 4, wherein the surfactant is polyoxyethylene tridecyl ether, Sophorolipid or a mixture thereof. The method of claim 1 or 2, wherein the polyoxyethylene lauryl ether, polyoxyethylene glycol, hydrotreated heavy paraffinic distillate, sodium benzoate benzoate) acaricide further comprising at least one selected from the group consisting of. The method of claim 4, wherein the polyoxyethylene lauryl ether, polyoxyethylene glycol, hydrotreated heavy paraffinic distillate, sodium benzoate Acaricides further comprising one or more selected from the group.