KR100836223B1 - Composition for killing sternorrhyncha comprising extracts of dryopteris crassirhizoma - Google Patents

Abstract

A composition comprising an extract of Dryopteris crassirhizoma is provided to control aphid environment-friendly without using a conventional chemical agent and decrease contamination by chemical materials and resistance against the chemical materials. A composition for killing Sternorrhyncha comprises an extract of Dryopteris crassirhizoma which is obtained by extracting rhizome of the Dryopteris crassirhizoma with an organic solvent such as methanol. The composition further comprises a vegetable oil spreader.

Description

Composition for aphids containing organic solvent extracts in spectators {COMPOSITION FOR KILLING STERNORRHYNCHA COMPRISING EXTRACTS OF DRYOPTERIS CRASSIRHIZOMA}

1 is a photograph of a cotton horse plant used in the present invention.

2 is a view of the cotton aphid control effect according to the spraying frequency of pangobi extract according to the present invention.

The present invention relates to a plant extract of the insecticides and aphids extracted from cotton horses (crowds, ferns, Dryopteris crassirhizoma) and a method for producing the same, and more specifically, insecticides and aphids extracted from the roots of cotton plant (spectrum) with an organic solvent It relates to a cotton horse plant extract and a method for producing the same.

Recently, the agricultural environment is getting worse due to the application of chemical pesticides for insecticides, aphids, etc. Among them, many problems are caused in the biological environment due to soil pollution and resistance to insects due to chemicals. In recent years, the market environment, in which the awareness of well-being foods has increased among consumers, neglecting fruits and vegetables using pesticides, has been increasingly emphasized.

Therefore, among farmers who grow fruits and vegetables, eco-friendly organic methods that do not use chemical pesticides are being studied. In particular, biomaterials related to aphids, which are studied a lot by organic methods, include garlic, green onions, peppers, morning glory, and herbal extracts.However, these methods are insect repellents and insecticides using individual characteristics of plants accumulated by the experiences of farmers. There is a lack of systematic research between each plant and aphid. Another eco-friendly biological material is a method of controlling aphids using natural enemies of pests such as aphids or mites, such as ladybugs (aphid nemesis). Caused.

Therefore, Gyeonggi-do Institute of Agricultural Research has conducted a systematic study on the extracts of cotton hemp plants for insecticides and aphids using the extracts of cotton hemp plants, especially pangobi rhizome, in connection with such environmentally friendly organic methods. In particular, the researchers made it easy to make pesticides and aphid preparations in general farms by using the roots of pangobi (crowd and polished), which are native to Korea, and these extracts of cotton horses are used for insecticides and aphids. It is intended to provide an excellent effect as a biologic.

The cotton horse (Dryopteris crassirhizoma, spectators) plants include pangori, tiger fern, etc. Similar species include D. lacera, D. uniformis, European spectator (D. filix- mas), North American spectators (D. marginalis) and African spectators (D. anthemintica). These crowds are mainly distributed in Korea and grow very much in mountainous areas such as Manchuria, similar to Korean soil.

The cotton aster plants are rooted in the rhizome and have a length of about 1m and a width of about 25cm, and the leaf disease is much shorter than the leaflets and dense with scales. The scales are glossy, yellowish brown or blackish brown, and the lower part is about 2cm long and has protrusions on the edges, but gradually narrows and becomes smaller toward the top. The leafs are lanceolate, deeply split into two idols, the postal leaves have no bands, and the width is 1.5-2.5cm, almost completely split into idols, and smaller toward the bottom, with wider intervals, and curls. The scales are long oval, coffee beans or dull heads with dull teeth at the edges, and the leaf veins enter from the surface, and the lateral veins are usually divided into two with dull hangs on the side. The spore cysts are attached in two lines near the middle of the upper right lobe of the upper leaf. Vesicle is round heart-shaped, flat at edge. In particular, the root is mainly used as a herbal medicine, the root of the grinding is called the spectator in the Chinese herbal medicine name, which is said to act on the liver to kill insects, lower the heat, release the poison and hemostatic action. Its roots are thick and straight, and have small roots extending in all directions and are perennial ferns. And grinding is only 50 to 100cm in size of the leaves exposed to the ground, the roots of the taste is bitter and cold ingredients and grows in a humid area contains a lot of moisture.

In particular, the main component of the rhizome or rhizome roots is filmarone, which is a complex phloroglucinol derivative that is unstable and slowly decomposes to form aspidin, albaspidin and aspidinol, flavaspidic acid and filicinic acid. In addition, it contains essential oils, fats and resins, and also contains triterpenoid fernene, hopene-b (diploptene), adiantone, hydroxyhopane (diplopterol) hopanol-29 (neriifoliol), and filicene. Root stocks of ferns contain ponasterone A, ecdysterone, and pterosterone. Roots of cotton plants contain ponasterone A and ecdysterone. Root stocks contain starch (26.5% or 48.5%) and tannin. Due to this component action, it is estimated that there is an antiparasitic action, antiviral action, antibacterial action. In particular, the roots of spectators paralyze veterinary muscles, myocardium, irritate the stomach, and in severe cases, it has been reported that there are poisonous ingredients that cause vomiting, diarrhea, and vision disorders, so they are not used in Korea's organic farming methods.

Accordingly, the present invention has been made to solve the above-mentioned problems, cotton pesticides for insecticides and aphids using extracts of cotton horse (Dryopteris crassirhizoma, spectators) plants, in particular pangobi root with respect to environmentally friendly organic farming and its preparation It is an object to provide a method.

Another object of the present invention is to provide a plant extract for insecticides and aphids, characterized in that a vegetable oil electrodeposition agent is added to the extract obtained by extracting dried cotton horseshoe roots with an organic solvent.

Still another object of the present invention is to put a 1: 10 weight ratio of methanol in the root diameter of dried pangobi and extract it for 48 hours at room temperature in a dark state, and the filtrate of the extracted extract is concentrated under reduced pressure at 40 ℃ lyophilized It is to provide a method for producing a cotton arachnid extract for insecticides and aphids, characterized in that the extract of the pancreatic rhizome (crude extract).

In connection with an environmentally friendly organic method according to the present invention for achieving the above object, cotton plant (Dryopteris crassirhizoma, spectators) plants, in particular insecticidal and aphid cotton plant plants extracts using the extract of pangobi root and its manufacturing method, The roots of pangobi (spectrum) are placed in methanol in a 1:10 ratio (1000 mL of methanol per 100 g of sample) and extracted for 48 hours at room temperature in a dark state. Thereafter, the extract was filtered with filter paper, and the filtrate was completely concentrated at 40 ° C using a vacuum concentrator. Crude extract (crude extract) dried in the freeze-drier was dissolved in 10% MeOH of the total dilution before the pest control effect test, and then the rest was dissolved in water (for example, 100mL after dissolving in 10mL methanol. Water). Then, 0.5% of vegetable oil (soybean oil, sunflower seeds, olive oil, etc.) serving as an electrodeposition agent is added. Mix thoroughly and spread evenly over plant foliar.

It demonstrates concretely based on an Example, a test example, and a drawing as follows.

Example 1 - Pangobi  Root Extract

The root diameter (root) of pango ratio (crowd) is put into methanol in a 1:10 ratio (1000 mL of methanol per 100 g of sample) and extracted for 48 hours in a dark room temperature. Thereafter, the extract was filtered with filter paper, and the filtrate was completely concentrated at 40 ° C using a vacuum concentrator. The concentrated extract was dried in a freeze dryer (crude extract) to dissolve in 10% MeOH of the total diluent before the pest control effect test, and then the rest in water dissolved (for example, 100mL prepared after dissolving in 10mL methanol. Water). Thereafter, only the pangobi rhizome extract is mixed evenly without spreading the electrodeposition agent and evenly sprayed all over the foliar surface of the plant.

Example 2  - Pangobi  Root Extract + Electrodepositable Vegetable Oil

After drying the extract (crude extract) as in Example 1 in 10% MeOH of the total dilution before the pest control effect test, and the rest in water, after adding 0.5% soybean oil as an electrodeposition agent. Mix thoroughly and spread evenly over plant foliar.

Test Example  One- Only obi high extract Cotton Aphid  Control effect

When aphid gossypii is grown to some extent on cucumber seedlings and pepper pots, or when artificially inoculates aphids at an appropriate density, the pre-density is investigated and the extracts are sprayed. It was. As for the red pepper field packaging, the control value was examined when the aphids reached the proper density. However, the pepper field packaging was tested on naturally occurring cotton aphids.

1. Cucumber seedling test

end. Test period: June ~ November 2005

I. Test place: Hwaseong pet house, Gyeonggi-do

All. Abandonment: 3 repetitions per treatment

la. Test plants: 3 ~ 5 mash of the main leaf, leaf irradiation (5-pot, baroque top set meal)

hemp. When to test: When 500 aphids per week

2. Pepper Pot Test

end. Test period: June ~ November 2006

I. Test Place: Hwaseong Vinyl House, Gyeonggi-do

All. Abandonment: 3 repetitions per treatment

la. Test plants: 7 ~ 10 mash of whole leaf (4 pots, baroque tops)

hemp. Test Period: When 300 to 400 aphids are formed per week

3. Red pepper field packaging test

end. Test Period: August 2006

I. Test Place: Hwaseong, Gyeonggi-do, Korea

All. Test aeration: 8 repetitions per treatment, 10 leaves irradiated (after labeling)

la. Test plants: When peppers are fully formed (when fully grown)

hemp. Test Period: When 400 to 700 aphids are formed per week

TABLE 1

Small neck Day 3 Day 7 % Viability Control price (%) % Viability Control price (%) Cucumber seedlings 91.0 53.0 67.6 71.8 Red pepper pot 31.5 81.9 56.1 72.3 Hot pepper packaging 57.9 64.9 48.1 61.5

※ 2 sprays  : 1 spray 3 days later  sparge

Test Example  2 - Vegetable oil  When mixed Cotton Aphid  Control effect

In the same way as above, when the aphid gossypii is grown to some degree on the seedlings of cucumber seedlings and red pepper pots, or when artificially inoculates aphids and reaches the proper density, the pre-density is investigated and the extracts are sprayed 3, 7 After the day, the respondents were investigated. As for the red pepper field packaging, the control value was examined when the aphids reached the proper density. However, the pepper field packaging was tested on naturally occurring cotton aphids.

TABLE 2

process Pre-Density (Marie / Leaf) Day 3 Day 7 % Viability Control price (%) % Viability Control price (%) Higher 0.5% 547.5 193.9 56.4 340.9 62.3 0.5% + 1% oil 987.0 58.2 86.9 119.7 86.8 Pesticides (chia methoxam) 2000 times 512.5 2.22 99.5 4.88 99.5 Oil 1% 356.5 210.3 52.7 445.0 50.8 Control (distilled water) 533.0 444.5 - 905.1 -

※ 2 sprays  : 1 spray 3 days later  sparge

Test Example  3- Gobi extract  By Solvent Cotton Aphid  Control effect Pot )

In the same way as above, when the aphid gossypii is grown to some degree on the seedlings of cucumber seedlings and red pepper pots, or when artificially inoculates aphids and reaches the proper density, the pre-density is investigated and the extracts are sprayed 3, 7 After days, the control agent was examined. As for the red pepper field packaging, the control value was examined when the aphids reached the proper density. However, the pepper field packaging was tested on naturally occurring cotton aphids.

TABLE 3

Extraction solvent Pre-density (mari) 3 days later 7 days later % Viability Control price (%) % Viability Control price (%) Methanol 132.3 43.7 73.0 68.4 69.5 ethanol 62.3 66.5 58.9 128.3 48.9 Acetone 170.7 112.8 30.4 153.3 31.6 chloroform 94.7 129.6 20.0 181.1 19.2 water 149.7 192.3 0 265.3 0 Control (distilled water) 110.7 162.1 - 224.2 -

※ 2 sprays  : 1 spray 3 days later  sparge

Test Example  4-number of sprays

As shown in Table 4 below, the number of times of spraying was divided and compared with the control group which is distilled water.

TABLE 4

Number of sprays Dictionary density (mari) % Viability 3 days later 7 days later 1 time 195.3 59.6 77.6 2 times (after 3 days) 182.0 71.5 18.8 Control (distilled water) 147.7 175.9 212.5

Test result

According to the control test example, the extracts of pangobi rhizome showed 71.8% in cucumber seedling assay, 72.3% in pots, and 64.8% in single spraying, but 90.7% in two sprays. It could be confirmed (see Fig. 2). According to the verification of the above control test effect, the control effect of pangobi root rhizome extract was confirmed that the effect is more excellent when only the extract of pangobi root rhizome is monotreated than mixed with vegetable oil, methanol in the extracted organic solvent When extracted with it was confirmed that the control effect is better. And when the number of times the spraying of the extract of the rhizome rhizome was confirmed that the cotton aphid control value increased to 90.7% when spraying twice, it can be confirmed that spraying twice as much as possible to control more effective aphids.

Peach black aphid (Myzus percicae), soybean aphid (Macrosiphum pisi), mite (Tetranycidae), and termite (Macroterminae) are believed to have the same effect on the chemicals used to control cotton aphids through the effect of controlling cotton aphids. ) Is also expected to have an excellent effect.

The above embodiments, test examples and drawings are merely for the purpose of describing the contents of the invention in detail, and are not intended to limit the scope of the technical idea of the invention, and the present invention described above is usually in the technical field to which the present invention belongs. As those skilled in the art, various substitutions, modifications and changes are possible within the scope without departing from the spirit of the present invention is not limited to the embodiments and the accompanying drawings, as well as the claims described below It should be determined that the claims are included in their equality.

In connection with such an eco-friendly organic method according to the present invention described above, by providing cotton plant extracts for insecticides and aphids using extracts of cotton horse plants, especially Pangobi root, and its manufacturing method, pangobi native to Korea It is possible to make pesticides and aphids that have excellent effects as pesticides and aphids by using the roots of spectators and cotton horses in general farms, and to make them and spray them so that they are not harmful like pesticides. By using commodity products, it is possible to produce well-being vegetables and fruit trees that are harmless to the human body while reducing pollution of the agricultural environment, thereby increasing farm income.

Claims (5)

A composition for aphids, characterized in that it comprises an organic solvent extract in the tube obtained by extracting the crowd with an organic solvent. The composition for aphids according to claim 1, wherein the root diameter of the tube is extracted with methanol. The composition for aphids according to claim 1 or 2, comprising a root extract of the tube and a vegetable oil electrodeposition agent. delete delete

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