KR20050082403A - The manufacturing process of bio-agricultural chemicals by use of exactracted fig material - Google Patents

Abstract

Particularly, the fig fruit extract contains a composition, and is intended to be used as a natural pollution-free pesticide for cultivation of overeats. Particularly, 0.1-10% of fig juice and pine needle solution and 0.1-1.0 weight of stabilizer are used for cultivation of horticulture, which can be expected to maintain freshness of sprouts and soybeans and prevent contamination and decay without using pesticides as an additive. A method for producing a natural pesticide composition containing an aqueous solution containing% as a stock solution as a manufacturing method, and a method for producing and cultivating fruits and vegetables, which is mixed with water and sprayed and supplied to the crop at a concentration of 0.01 to 1%.

Description

The manufacturing process of bio-agricultural chemicals by use of exactracted Fig material}

The present invention is obtained by extracting and extracting the active substance through the organic solvent, that is, methanol and the like by the deposition method and the extraction method using the fig extract and juice extract, and in particular to separate the fig extract component according to the antibacterial test results It relates to the production of natural pollution-free pesticides for plant crop cultivation, using the fig active material. Fig. Is also recorded as a fruit with high sugar content and activity enough to be widely used for medicinal purposes. Figs (Ficus carrical L) are widely used as processed foods because of their high activity, and unlike other fruits, figs are fruits that are completely pollution-free and can be grown without using pesticides. Thus, figs have a strong active substance against their own pests, and may contain various bioactive components such as ficin, vitamins, and minerals. As such, microorganisms and pests that propagate in food or crops vary according to climate and environmental conditions, but most of them are in need of removing microorganisms due to microbial decay and the development of pest repellents and development of new drugs according to defense mechanisms. Has become an important research subject. In recent years, excessive pesticide application has caused excessive pesticide residues from agricultural products, so that the mass media has been raised and lowered. Therefore, the limited regulation of foodstuffs poses a health threat to pregnant women as well as newborns. Therefore, the development of pollution-free natural pesticides is indispensable and should be encouraged at the national level.

In particular, figs have been used as a hemorrhoids and athlete's foot treatment and insect repellent, its pharmacological effect is recognized and there are many active substances in fig fruit. Therefore, in this study, based on the results of the existing extraction and separation research developed by the present inventors, natural pesticides using a complex active substance were developed by directly using a substance which is a natural antimicrobial agent obtained from figs.

The present invention relates to a method for producing natural pesticides used in crops such as bean sprouts and overgrowths, which grow faster and grow faster, without preventing disease and pests, and without infecting common germs using an aqueous solution of fig extract.

Excessive toxic pesticides, various plant growth hormones, synthetic antibiotics, heavy metal mixed fertilizers, etc. are used for the purpose of speeding up the growth of sprouts and preventing various diseases.

However, the use of various additives and spreading agents used in growing crops is advantageous from the grower's point of view, but the health of consumers who consume bean sprouts and vegetables directly is toxic such as residual pesticides. It acts as a threat. For example, the indole ratio (indol-3-yl acetic acid), which is excessively used in bean sprouts plants, is currently extremely limited and is only allowed within a certain allowable range because of its high toxicity and strong effect on the human body. At present, the trend is to completely ban the use, but it is possible to reduce the cost of cultivation by enabling the prevention of various diseases of the bean sprouts and the cultivation of properties, and in some cases, it is still used to avoid crackdowns. Therefore, the present invention relates to a method for producing natural pharmaceuticals for crop cultivation having an extract using an organic solvent using figs and the like and promoting natural plant growth and using natural pollution-free pesticides.

Therefore, the present inventors have applied various mixtures and fractions obtained by extracting active ingredients from edible natural figs to solve the above-mentioned conventional crop cultivation problems, but harm various conventional plant growth promoters. Fast growing and antimicrobial activity for growing bean sprouts and crops without pests by using no-toxic pesticides and antibiotics, without using toxic pesticides and antibiotics, especially when used as pest control and insecticide agonist And a pollution-free natural pesticide for growing vegetable crops, which is a natural pesticide agent having increased stability.

The present invention uses a fig mixed solution containing 20% of fig raw liquid preparation and fig fermentation liquid using a juice or extract, such as Seungui Woopin (Masui Dauphine), Bongsi or White Genoa species, Bongasi fig fruit.

More specifically, the method includes diluting the fig extract with 0.01-10% and spraying water at a concentration of 0.01-0.5%.

Accordingly, an object of the present invention is to develop a formulation containing an active substance extracted from figs, which is a natural product and a pollution-free resource, and the above-mentioned natural product supplement.

In the application method, the aqueous solution of fig was used for growing bean sprouts, and the germinated growth pattern of the seed treated with the fig was also the root, but the trunk was slightly thicker. There was no rotten disease in the stems and roots of the bean sprouts due to increased antimicrobial activity.

When the fig extract is used as in the present invention, since the antimicrobial properties of the figs and the like do not cause the decay of the viscous substances released or secreted from the bean sprouts, it can be used repeatedly several times. Therefore, when growing bean sprouts, the amount of water can be reduced, and the waste water discarded is relatively reduced.

The present invention extracts and fractions the waste fig fruit by using an organic solvent and extracts or extracts the fig fruit, which is unripe and ripe fruit, by using a compound, and extracts or extracts these figs into a pulverized powder. Also included in the present invention is a method for use as a pesticide and other feed for use by grinding and extracting or fermenting with water or the like. And the juice is extracted and extracted separately with methanol and used in the preparation of natural pesticides according to the mixing ratio in the mixed aqueous solution. In addition, the present invention invented a method and efficacy of natural pesticide preparations containing insecticides and pest control agents and antimicrobial agents by using the method of extracting or extracting complex with the natural pesticides. In particular, the preferred temperature during the production is concentrated under reduced pressure in the low temperature range of 20-70 degrees. Also preferred is a temperature of 40-70 degrees.

The present invention is obtained by using a fig fruit which is a natural substance, a mixed extract using an organic solvent water, immersion method, an electrodeposition method, and a pulverization method using methanol, and selectively performing it at a temperature of 30-90 degrees, a more preferable temperature range Concentrated under reduced pressure for 2 hours to 36 hours at 50-70 degrees and prepared as a raw material according to each embodiment by using the additives and the fig extract obtained by extracting the raw fig juice according to the method of obtaining the emulsion and natural according to the processing procedure Prepare and process antimicrobial agents. And 0.1 to 7.0 parts by weight and 0.1 to 1.0 parts by weight of a stabilizer, respectively.

The present invention relates to an antimicrobial composition comprising from 0.1 to 7.0 parts by weight of a natural fig extract and from 2 to 25% by weight of a fig juice concentrate. In the compositions such as fig fruit according to the present invention, when the extract and the fig juice concentrate of the active ingredient fig active ingredient is used in more than 25 parts by weight, the general antimicrobial agent of the fig activity is viscous to produce agglomeration, and the production method and storage life is poor. In this case, incidental solution stability is lowered, such as having to mix with water again. For this reason, it is reasonable to develop a fig antimicrobial solution that can be used by mixing dispersant with a high concentration of fig concentrate.

The fig stabilizer according to the invention is from 0.1 to 1.0 and the aspartic acid, gulutamide acid or surfactant is from 0.02 to 0.04.

In the present invention, a stabilizer may be used for stabilizing the fig separation component. Stabilizers are used by selecting one or two of commonly used pollution-free aspartic acid, glutamic acid, sodium carbonate, and surfactants. Fig. The pesticide composition of the present invention is preferably used 0.1 to 1.0 parts by weight of a stabilizer. When 0.1 part by weight or less of stabilizer is used, sufficient stabilization effect cannot be expected. Hereinafter, the present invention will be described in detail with reference to Examples, but the technical scope of the present invention is not limited to these Examples.

As already mentioned, the fig component prepared according to the present invention has its own antibacterial and antifungal activity, and can be used as a fungicide as a prophylactic and therapeutic agent as well as to combat antifungal antifungal.

As a known and useful natural pesticide, it targets the common fungi Candida albigans, Sagaromyces cerevisiae and Aspergillus niger and Gram-negative bacteria G (-) Aureus coli ATCC10536 As a result of investigating the antimicrobial effect of the target compound according to the present invention, it can be confirmed that the prepared compound is a natural product in the candida fungus than the common fungicide ketoconazole agent against bacteria, but excellent antimicrobial activity. Therefore, the novel chitosan-alginic acid-figure compound compound is an antimicrobial agent having a well-known antifungal and antimicrobial efficacy and a pharmacological effect.

The complex compound according to the present invention may be administered in a pharmaceutically acceptable suitable dosage form prepared using a pharmaceutically acceptable additive and a suitable carrier, and comprises a composition containing the infusion fluid compound compound as an active ingredient at a predetermined dilution ratio. Therefore, it is sprayed with natural pesticides. Depending on the application method, it is used as a fruit coating and seed germination disinfection and accelerator.

Minimum Inhibitory Concentration (MIC) for Natural Biopesticides in vitro ) Measurement

1. Use strain

The microorganisms used in this experiment were Escherichia coli ATCC 10536, Pseudo monas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 2 5923, Bacillus subtilis ATCC 6633, G. Candida utilis, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763, Aspergillus niger 9029, Candida pseudotropicalis, Tourlopsis glabrata 89-5 were used.

2. Medium used

Bacterial media used in this experiment: Mueller Hinton broth, Mueller Hinton ager and fungal media: Sabouraud Dextrose Broth, Sabouraud Dextrose agar.

3. Preparation of each extract and assay medium and measurement of antimicrobial activity:

One). To make the fungal diluent, the whole culture is diluted to a concentration of 104-106 CFU / ml.

2). Each sample is dissolved in a suitable solvent and then diluted in 2-fold dilution with medium.

3). Inoculate each test tube with bacterial solution.

4). Bacteria are incubated at 37 degrees for 24 hours and fungi are incubated for 36-48 hours.

5). After each incubation, shake the test tube to check the turbidity of the bacteria. After checking the turbidity, each was inoculated in Mueller Hinton ager, Sabouraud Dextrose agar medium.

6). After confirming the growth of the bacteria, determine the test tube concentration of the remaining concentration that is not recognized growth, and determine the MIC by the broth serial dilution method as described above.

4. Antimicrobial activity of each compound isolated:

In vitro antimicrobial activity test (Minimum Inhibition Concentration) of (Example 5 and the control natural pesticides and ketoconazole as a comparative compound) is shown in the table below.

[See Fig. 2] (Unit: g / ml)

<Production Example 1>

The present invention relates to the manufacture of natural pesticides using figs and experimented with fig fruits of Seunguiwooup (Masui Dauphine), Bonglai or White Genoa species, which are cultivated and produced in Sanho-myeon, Samho-myeon, Yeongam-gun, Jeonnam. The extracts were extracted or extracted with methanol, ethanol and other organic solvents by dipping and pressing, respectively, and concentrated under reduced pressure at 30-70 degrees to prepare a sample. As a specific method, fig fruit is used as it is in water, methanol, etc., based on 10 kg. For example, 10-50 times the amount of solvent is added to extract the solvent by immersion compression method or pulverization method at room temperature for at least 3 months, and then the solvent is removed using a vacuum concentrator at 25-70 degrees and stored in the refrigerator as a sample. Was used.

<Production Example 2> Preparation of Juice Extract

Figs were washed and dried, and then ripened figs, immature fruits and fresh fruits were pulverized separately based on 10 kg using 2-10 times the selected organic solvent, and then juiced using a compress. And it was concentrated using a vacuum concentrator at a temperature of 25-70 degrees, stored in a refrigerator and tested.

Example 1

Put the waste fig in the mill and grind it well, and then add about 10 times the volume of methanol and leave it for about 30 days at 25-70 ° C to extract enough figs. It was efficient when extracted. Juice was carried out using a press. And the filter was concentrated using a vacuum condenser at a temperature of 25-70 degrees, stored in a refrigerator and tested.

Example 2

After soaking in 0.05-2% of the fig extract for 2 hours, the soybeans were placed in a hydroponic cultivation bowl, and the aqueous solution of figs was continuously sprayed onto the bean sprout siru. Meanwhile, the growth rate was compared and examined while growing bean sprouts using water in the same manner.

The growth rate was compared with the change of bean sprout growth weight. As a result, after 8 hours of cultivation of sprouts, cultivation with an aqueous solution of figs than the sprouts grown with water completely eliminated the infection with fungi, and the black sprouts, brown strains, etc. were completely removed from the sprouts. The growth rate was about 81% faster.

Example 3

As a result of spraying 0.05 ~ 0.2% of the fig extract three times, the freshness of the bean sprouts was maintained for more than 90% for 100 hours.

Example 4

Fig. 0.01-10% (figure 5%) of fig extract according to the invention and 0.1-10% (2% each at the time of manufacture) and 0.1% by weight of an aqueous solution containing a stabilizer prepared by storing the stock solution used for storage It was.

Example 5

10% fig extract according to the present invention and a composition containing an aqueous solution containing 0.5% and 0.1-1.0% by weight of each stabilizer with other compositions, containing a method of diluting it with water and spraying at a concentration of 0.01 to 0.3% do.

As described in detail above, according to the present invention, in the case of using the natural pesticide containing the bean sprouts, especially the fig component itself as an insecticide and an antimicrobial activity as an antimicrobial substance, it has the effect of promoting the growth of plants and combating the decay disease and the insecticide and pest control effect. When it is farmed, it has excellent efficacy as a natural pesticide and can greatly contribute to the health of the people.

1 is a fig.

Figure 2 shows the results of in vitro antimicrobial activity of natural biological pesticides

Claims (2)

Containing a fig fruit extract and 5% fermentation broth, containing 0% of pine needles and 0.01-0.05% by weight of an aqueous solution containing 0.01-0.05% by weight of stabilizer as a stock solution And a vegetable and vegetable production process and a cultivation method of diluting the preparation composition with water and spraying and supplying the crop with a 0.01 to 1% concentration composition. Fig. 1 extract the fig fruit with an organic solvent or by using an organic solvent to extract juice by hydraulic pressure, concentrated under reduced pressure, or crushed figs or fermented at 25-29 degrees to extract with an organic solvent or directly extracted with an organic solvent to extract and extract natural extracts, etc. How to use and use as animal feed for livestock