CN106665665B - A kind of Pesticidal combination and the preparation method and application thereof - Google Patents
A kind of Pesticidal combination and the preparation method and application thereof Download PDFInfo
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- CN106665665B CN106665665B CN201611128510.2A CN201611128510A CN106665665B CN 106665665 B CN106665665 B CN 106665665B CN 201611128510 A CN201611128510 A CN 201611128510A CN 106665665 B CN106665665 B CN 106665665B
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- celastrus angulatus
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- 230000000361 pesticidal effect Effects 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 239000000284 extract Substances 0.000 claims abstract description 106
- 241000296609 Celastrus angulatus Species 0.000 claims abstract description 72
- 235000003325 Ilex Nutrition 0.000 claims abstract description 69
- 241000209035 Ilex Species 0.000 claims abstract description 69
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims abstract description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000000839 emulsion Substances 0.000 claims abstract description 24
- 210000000481 breast Anatomy 0.000 claims abstract description 16
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 16
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 16
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 16
- 239000000470 constituent Substances 0.000 claims abstract description 11
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 12
- BMRWNKZVCUKKSR-UHFFFAOYSA-N butane-1,2-diol Chemical compound CCC(O)CO BMRWNKZVCUKKSR-UHFFFAOYSA-N 0.000 claims description 11
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- 241000345998 Calamus manan Species 0.000 claims description 4
- 235000012950 rattan cane Nutrition 0.000 claims description 4
- 230000001502 supplementing effect Effects 0.000 claims description 3
- 241001106041 Lycium Species 0.000 claims description 2
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- 229940083957 1,2-butanediol Drugs 0.000 claims 2
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- 239000002023 wood Substances 0.000 abstract description 6
- 230000000857 drug effect Effects 0.000 abstract description 5
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- 244000144972 livestock Species 0.000 abstract description 3
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- 241000402102 Paratrioza sinica Species 0.000 description 14
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- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
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- 241001655728 Celastrus Species 0.000 description 1
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- 230000009471 action Effects 0.000 description 1
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- 239000007798 antifreeze agent Substances 0.000 description 1
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- 239000003124 biologic agent Substances 0.000 description 1
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- 238000001802 infusion Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000009916 joint effect Effects 0.000 description 1
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 235000017807 phytochemicals Nutrition 0.000 description 1
- 229930000223 plant secondary metabolite Natural products 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
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- 239000002904 solvent Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
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- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/14—Celastraceae [Staff-tree or Bittersweet family], e.g. spindle tree, bittersweet or thunder god vine
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of Pesticidal combination, active constituent is root bark of celastrus angulatus extract and Chinese ilex root extract, preparation method are, comprising the following steps: 1) root bark of celastrus angulatus extract, Chinese ilex root extract is mixed to obtain oily phase with cyclohexanone；2) by agriculture breast 2201, sodium benzoate, ethylene glycol, 1, the 1/2 of 2-butanediols and water consumption mixes to obtain water phase；3) water phase is added in oily phase and is mixed, while adding remaining water to get aqueous emulsion.Pesticidal combination of the invention is aqueous emulsion, environmentally safe using water as matrix, nonflammable, and storing is convenient；Compared with single dose, drug effect height, lasting period length, the generation for having delayed pest resistance to insecticide；Antagonism aphid, wood louse have good control efficiency；Effective component can degrade in nature, and being compatible with the environment property is good, noresidue, pollution-free, safe and environment-friendly, the features such as to safety of human and livestock.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to one kind contains root bark of celastrus angulatus extract and Chinese ilex root extract
Pesticidal combination and the preparation method and application thereof.
Background technique
In recent years, the weight that high poison, high residue chemical pesticide become pollution of ecological environment, endanger food safety and common people's health
Want problem.With the understanding that people endanger chemical pesticide, country has been limited toxic in World Developed Countries and partial development
The use of chemical pesticide, many countries, which have formulated, reduces the countermeasure that chemical pesticide uses, and proposes the substitute products of chemical pesticide.In
State is a large agricultural country, and pesticide plays highly important effect in the agricultural production in China.Stringent limitation uses chemistry
Pesticide solves excessive pesticide residues, improves the competitiveness demand of the safety and agricultural products in China of agricultural product in international market and compels
It cuts.
To adapt to China's agricultural sustainable development strategy, the developing direction of pesticide will turn to selection from non-selective pesticide
Property pesticide, " the reasonable pesticide of ecology " and " environment harmony pesticide " are turned to from traditional organic chemicals, in favor of protection environment,
Promote the sustainable development of agricultural.Therefore, many biological pesticides are due to having selectivity strong, to safety of human and livestock, to non-target life
The influence of object is smaller, is able to maintain balance of nature；It is easy to degrade in the natural environment, noresidue is free from environmental pollution；It is a variety of because
Element and ingredient play a role, and harmful organism is difficult to the advantages that developing drug resistance and is paid more and more attention (Xu Hanhong, Zhang Zhixiang, journey
Eastern beauty.Advantages for development of biological pesticide and there are problems.World pesticide, 2004,26 (2): 5-10).
Botanical pesticide is one of biological pesticide most ancient in human history, it is from nature, in the natural environment
Degradable, safety nuisance free has become one of green bio pesticide first choice.Phytochemicals production Celastrus angulatus is from tradition
Pesticide plant Celastraceae Celastrus Celastrus angulatus (Celastrus angulatusMax.) (Wu Wenjun, 1991).Natural resources
It is abundant, it is widely distributed in the mountain area and hills of the Yellow River, the Yangtze river basin, reserves are abundant, according to investigations the Celastrus angulatus in xuan-en, hubei county point
Cloth area accounts for the 23.12% of whole county mountainous region area, in Shaanxi Hua County Celastrus angulatus reserves up to 2000 t or so, seed in terms of dry root skin
At least produce 500 t per year, and Celastrus angulatus can normal growth in clay, sand and rock ballast.According to surveying and determination, average every 100 kg is fresh
26.2 kg of Gen Ke get does root skin, and well developed root system, environment-adapting ability and power of regeneration are all very strong, excavates second year after main root
Many new plant (Wang Yunfeng, Shi Weiyong out can be sprouted from the radicula stayed in the soil.The exploitation benefit of Chinese Insecticidal Plant Resource
With.Resources science, 2001,23(2): 62-64).And its current artificial breeding technology mature (Lu Lingxian, Nan Yusheng, Ke
It manages state affairs, Wan Caigan, Xu Huizhu, Wu Dongyun.Insecticidal Plant Celastrus Angulatus introduces a fine variety study on reproduction preliminary study.Hubei forestry science and technology, 1987,61
(3): 8-10).
Applicant combines the great demand of international advanced technology and domestic and international market, has been actively developed botanical pesticide-day
Right Celastrus angulatus LS20030503.The natural Celastrus angulatus of botanical pesticide is to the aphids such as wheat aphid, Paratrioza sinica, Wolfberry Aphid section elder brother
Worm has certain control efficiency, but its single dose quick-acting is poor, and there is slow to pest drug effect, field efficacy is general, spray
Often, the defects of extension rate is lower.It is considered as the effective measures for overcoming or delaying pest resistance that insecticide combination, which uses,
One of, mixed pesticide is to utilize and be transformed the old kind of pesticide, improves a kind of effective way of its control efficiency, has important reality
Trample meaning.Therefore, this research department proposes there is the natural of given efficacy with active constituent group and the research of component compatibility theory, screening
At grouping, the exploitation of novel plant source pesticide is carried out with this theory, it is more can preferably to play vegetable source natural active constituent group
Ingredient, multiple target point, synergistic advantage, solve the problems, such as that botanical pesticide activity is relatively low, quick-acting is poor.
Summary of the invention
The object of the present invention is to provide a kind of good disinsection effect, drug cost is low, noresidue, it is pollution-free, to safety of human and livestock,
It is not likely to produce the Pesticidal combination containing root bark of celastrus angulatus extract and Chinese ilex root extract of drug resistance, utilizes two kinds of plant sources
Pesticide has complementary advantages, and sufficiently improvement root bark of celastrus angulatus extract quick-acting is poor, and drug effect is slow, knocks down the defects of rate is relatively low, together
When provide preparation method and application be another goal of the invention of the invention.
Based on above-mentioned purpose, this invention takes following technical solutions: a kind of Pesticidal combination, and active constituent is bitter skin
Rattan root bark extract and Chinese ilex root extract.
The weight proportion of root bark of celastrus angulatus extract and Chinese ilex root extract is 1~4:1~4.
The root bark of celastrus angulatus extract is obtained via following steps: root bark of celastrus angulatus crushed, root bark of celastrus angulatus powder is obtained,
The ethyl alcohol that 3~5 times of root bark of celastrus angulatus powder weight is added is concentrated under reduced pressure after filtering in 60~80 DEG C of 1~4h of ultrasonic extraction to obtain the final product；
The Chinese ilex root extract is obtained via following steps: Chinese ilex root being crushed, the acetic acid second of 3~6 times of Chinese ilex root powder weight is added
Ester is concentrated under reduced pressure after filtering in 50~80 DEG C of 3~5h of ultrasonic extraction to obtain the final product.
The Pesticidal combination is aqueous emulsion, weight percent composition are as follows: active constituent 5~15%, cyclohexanone 15~
30%, sodium benzoate 0.5~1.2%, 0.5~1.2%, 1,2-butanediol 0.5~1.5% of ethylene glycol, agriculture breast 2201 are 3~5%,
Yu Weishui.
The weight percent of the aqueous emulsion forms are as follows: root bark of celastrus angulatus extract 5%, Chinese ilex root extract 5%, cyclohexanone
For 30%, sodium benzoate 0.9%, ethylene glycol 0.8%, 1,2-butanediols are 1.2%, agriculture breast 2201 is 4.2%, remaining is water.
The preparation method of the Pesticidal combination, comprising the following steps:
1) root bark of celastrus angulatus extract, Chinese ilex root extract are mixed into obtain oily phase with cyclohexanone；
2) by agriculture breast 2201, sodium benzoate, ethylene glycol, 1, the 1/2 of 2-butanediols and water consumption mixes to obtain water phase；
3) water phase is added in oily phase and is mixed, while adding remaining water to get aqueous emulsion.
10~30 min are stirred with the speed of 800~1000 r/min when mixing in step 1) and step 2；Step 3)
10~30min is stirred when mixing under the high speed of 10000~12000 r/min, while supplementing the water of surplus.
The application of the Pesticidal combination is applied to the prevention and treatment to Lycium Pests.
In the present invention, each component role are as follows: cyclohexanone is solvent；Sodium benzoate is preservative；Ethylene glycol is anti-
Freeze agent；1,2-butanediol is stabilizer；Agriculture breast 2201 is emulsifier.
Compared with prior art, the beneficial effects of the present invention are:
The present invention be directed to root bark of celastrus angulatus extract and Chinese ilex root extract single dose, that there are quick-acting is poor, to pest medicine
Effect is slow, field efficacy is general, spray often the defects of, it is theoretical to introduce traditional Chinese medicine, to root bark of celastrus angulatus extract and Chinese ilex
Root extract carries out active constituent group compatibility, to achieve the purpose that two kinds of botanical pesticides have complementary advantages, sufficiently improves Celastrus angulatus
Root bark extract quick-acting is poor, and drug effect is slow, knocks down the defects of rate is relatively low.To so far, have no root bark of celastrus angulatus extract
Object and Chinese ilex root extract compounding report.And the present invention and through multiple repetition test, achieve good effect；
1, obtained aqueous emulsion is environmentally safe using water as matrix, nonflammable, and storing is convenient；
2, compared with single dose, drug effect height, lasting period length, the generation for having delayed pest resistance to insecticide；
3, antagonism aphid, wood louse have good control efficiency；
4, effective component can degrade in nature, and being compatible with the environment property is good, noresidue, pollution-free, safe and environment-friendly, to people
Raise the features such as safe.
Specific embodiment
Below will be by specific embodiment come the present invention will be described, but these specific embodiments are not in any way
It limits the scope of the invention.It should be noted that it is convenient for statement, it is all made of " bitter Chinese ilex " in following embodiment and comes
It indicates " root bark of celastrus angulatus extract Chinese ilex root extract "
Embodiment 1:5% hardship Chinese ilex aqueous emulsion
A kind of Pesticidal combination is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract 4%, Chinese ilex root
Extract 1%, cyclohexanone 15%, sodium benzoate 0.5%, 0.5%, 1,2-butanediol 0.5% of ethylene glycol, agriculture breast 2201 are 3%, remaining
For water.
The preparation method of the Pesticidal combination, comprising the following steps:
1) root bark of celastrus angulatus extract, Chinese ilex root extract are mixed into obtain oily phase with cyclohexanone；
2) by agriculture breast 2201, sodium benzoate, ethylene glycol, 1, the 1/2 of 2-butanediols and water consumption mixes to obtain water phase；
3) water phase is added in oily phase and is mixed, while adding remaining water to get aqueous emulsion.
20 min are stirred with the speed of 900 r/min when mixing in step 1) and step 2；Step 3) mix when
20min is stirred under the high speed of 11000 r/min, while supplementing the water of surplus.In other embodiments, step 1) and step
10~30 min are stirred using the speed of 800~1000 r/min when rapid 2) middle mixing；When step 3) mixes 10000~
10~30min is stirred under the high speed of 12000 r/min.
Wherein, root bark of celastrus angulatus extract is obtained via following steps: root bark of celastrus angulatus being crushed, root bark of celastrus angulatus is obtained
The ethyl alcohol (mass concentration of the ethyl alcohol be 95%) of 4 times of root bark of celastrus angulatus powder weight is added in powder, in 70 DEG C of ultrasonic extraction 2h,
It is concentrated under reduced pressure into paste crude extract after filtering to obtain the final product；The Chinese ilex root extract is obtained via following steps: Chinese ilex root crushed,
The ethyl acetate that 5 times of Chinese ilex root powder weight is added is concentrated under reduced pressure into paste crude extract i.e. in 70 DEG C of 3 h of ultrasonic extraction after filtering
?.
Embodiment 2:8% hardship Chinese ilex aqueous emulsion
A kind of Pesticidal combination is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract 6%, Chinese ilex root
Extract 2%, cyclohexanone 16%, sodium benzoate 0.8%, 0.7%, 1,2-butanediol 0.8% of ethylene glycol, agriculture breast 2201 are 3.5%,
Yu Weishui.
The preparation method of the Pesticidal combination, difference from Example 1 are: mixing in step 1) and step 2
Shi Jun stirs 30 min with the speed of 800 r/min；Step 3) stirs 30min under the high speed of 10000 r/min when mixing.It is bitter
When prepared by skin rattan root bark extract, the ethyl alcohol of 3 times of root bark of celastrus angulatus powder weight is added, in 60 DEG C of 4 h of ultrasonic extraction；Chinese ilex root mentions
When object being taken to prepare: the ethyl acetate of 3 times of Chinese ilex root powder weight is added, in 50 DEG C of ultrasonic extraction 5h.Remaining is the same as embodiment 1.
Embodiment 3:6% hardship Chinese ilex aqueous emulsion
A kind of Pesticidal combination is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract 4%, Chinese ilex root
Extract 2%, cyclohexanone 24%, sodium benzoate 0.7%, 0.6%, 1,2-butanediol 0.7% of ethylene glycol, agriculture breast 2201 are 3.8%,
Yu Weishui.
The preparation method of the Pesticidal combination, difference from Example 1 are: mixing in step 1) and step 2
Shi Jun stirs 10 min with the speed of 1000 r/min；Step 3) stirs 10min under the high speed of 12000 r/min when mixing.
When prepared by root bark of celastrus angulatus extract, the ethyl alcohol of 5 times of root bark of celastrus angulatus powder weight is added, in 80 DEG C of 1 h of ultrasonic extraction；Chinese ilex root
When prepared by extract: the ethyl acetate of 6 times of Chinese ilex root powder weight is added, in 80 DEG C of ultrasonic extraction 3h.Remaining is the same as embodiment 1.
Embodiment 4:10% hardship Chinese ilex aqueous emulsion
A kind of Pesticidal combination is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract 5%, Chinese ilex root
Extract 5%, cyclohexanone 30%, sodium benzoate 0.9%, 0.8%, 1,2-butanediol 1.2% of ethylene glycol, agriculture breast 2201 are 4.2%,
Yu Weishui.
The preparation method is the same as that of Example 1 for the Pesticidal combination.
Embodiment 5:9% hardship Chinese ilex aqueous emulsion
A kind of Pesticidal combination is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract is 3%, Chinese ilex
Root extract is 6%, cyclohexanone 27%, sodium benzoate 0.8%, ethylene glycol 0.8%, 1, and 2-butanediols are 0.9%, agriculture breast
2201 be 4%, remaining is water.
The preparation method is the same as that of Example 1 for the Pesticidal combination.
Embodiment 6:12% hardship Chinese ilex aqueous emulsion
A kind of Pesticidal combination is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract 3%, Chinese ilex root
Extract 9%, cyclohexanone 30%, sodium benzoate 0.9%, 0.9%, 1,2-butanediol 1% of ethylene glycol, agriculture breast 2201 are 4.5%, remaining
For water.
The preparation method is the same as that of Example 1 for the Pesticidal combination.
Embodiment 7:15% hardship Chinese ilex aqueous emulsion
A kind of Pesticidal combination is aqueous emulsion, weight percent composition are as follows: root bark of celastrus angulatus extract 3%, Chinese ilex root
Extract 12%, cyclohexanone 30%, sodium benzoate 1.2%, 1.2%, 1,2-butanediol 1.5% of ethylene glycol, agriculture breast 2201 are 5%, remaining
For water.
The preparation method is the same as that of Example 1 for the Pesticidal combination.
8 effect experiment of embodiment
For the technical effect for verifying products obtained therefrom of the present invention, inventor has carried out multiple repetition test, specific as follows.
Experimental method: take root bark of celastrus angulatus extract and Chinese ilex root extract by weight ratio be respectively 4:1,3:1,2:1,
The extracting method of 1:1,1:2,1:3,1:4, root bark of celastrus angulatus extract and Chinese ilex root extract is the same as above-described embodiment 1, Mei Gepei
Than being diluted with distilled water into 5 concentration respectively, every processing is repeated 4 times, and sets blank control.
8.1 pairs of Wolfberry Aphid indoor toxicity tests:
Test worm processing uses infusion process.The fresh fructus lycii tender tip with aphid is adopted, winged aphids and extra aphid are rejected,
Keep aphid size almost the same, then it is impregnated 5 s in medical fluid and is put into after taking-up is dried and has been lined with filter paper by medium density
Diameter be in the culture dish of 9 cm, culture dish adds water moisturizing, be placed in RXZ intelligence artificial lighting climate box, temperature (25 ±
0.5) DEG C, relative humidity (RH) 60%, illumination L: D are 16 h: 8 h.The dead borer population of each processing is checked after 24 h, and calculates correction
The death rate.Death standard touches aphid body with writing brush, is dead (Du Yuning, Zhang Zongshan, Shen Ruiqing, height with the inactive person of aphid foot
It is beautiful.Indoor toxicity test [J] of 4 kinds of biological pesticides to Wolfberry Aphid.Forestry science and technology, 2007,32 (6): 29-30).
The gained death rate is corrected with Abbott formula, according to drug concentration logarithm and corrected mortality probit value
Return law of the straight line, calculate virulence regression equation, the lethal concentration of 50 (LC50).Mixture is found out using (1960) formula such as Sun Yunpei
Co-toxicity coefficient (CTC) (Sun Y P, E R Johnnson. Analysis of joint action of pesticides
Against house files [J] Ecological Entomology, 1960,53:887-891).Synergy judgement mark
Quasi-: it is significant synergy that co-toxicity coefficient (CTC), which is greater than 200, and 150~200 is smaller for synergistic effect, and 70~150 be summation action, small
It is antagonism (Tan Fujie, Shen Jinliang, You Ziping in 70.The research [J] of insecticide mixing application.Agricultural University Of Nanjing's report,
1987,4 (increasings): 93-99).
In formula: A indicates A single dose toxicity index；ACIndicate A shared percentage in mixture；B indicates B single dose toxicity index；
BCIndicate B shared percentage in mixture.
Root bark of celastrus angulatus extract and Chinese ilex root extract are to the virulence of Wolfberry Aphid malicious coefficient determination calculated result together
It is shown in Table 1:
1 root bark of celastrus angulatus extract of table and Chinese ilex root extract are to the virulence of Wolfberry Aphid malicious coefficient determination result together
。
The measurement result of table 1 shows: root bark of celastrus angulatus extract and two kinds of group of effective components of Chinese ilex root extract are by table 1
Ratio compounding after have a significant synergistic effect to Wolfberry Aphid, root bark of celastrus angulatus extract and when Chinese ilex root extract 1:1 increases
Effect acts on most strong, co-toxicity coefficient 265.27；When ratio is 4:1,3:1,2:1,1:2,1:3,1:4, co-toxicity coefficient is followed successively by
191.76、210.68、232.85、228.87、199.73、183.57。
8.2 pairs of Paratrioza sinica indoor toxicity tests:
The wolfberry leaf with wood louse is taken, the wood louse similar in writing brush picking individual size is uniformly placed on face of blade
And each small on piece is made to have wood louse 20~30, blade is immersed in prepared pesticide solution, is taken out after impregnating 5 s,
Extra medical fluid is sucked with blotting paper and is dried, and is then placed in the culture dish for spreading 90 mm of the wet water absorbent paper that haves three layers, it is wet with 2 layers
Culture dish lid is covered after gauze sealing, room temperature is maintained at (26 ± 1) DEG C.Every processing is repeated 3 times, and is control with clear water.
Total borer population and dead borer population are investigated after 24 d.Death standard is to touch polypide and appendage with small writing brush, and no any reaction is then considered as dead
Die (Guan Xiaoqing.The screening and natural enemy Study of Sensitivity of Paratrioza sinica biological agent.Agriculture of Anhui science, 2009,37(10):
4541-4542).Calculate virulence regression equation, the lethal concentration of 50 (LC50) and co-toxicity coefficient.
Root bark of celastrus angulatus extract and Chinese ilex root extract are to the virulence of Paratrioza sinica malicious coefficient determination calculated result together
It is shown in Table 2.
2 root bark of celastrus angulatus extract of table and Chinese ilex root extract are to the virulence of Paratrioza sinica malicious coefficient determination result together
。
The measurement result of table 2 shows: root bark of celastrus angulatus extract and two kinds of group of effective components of Chinese ilex root extract are by table 2
Ratio compounding after have a significant synergistic effect to Paratrioza sinica, root bark of celastrus angulatus extract and when Chinese ilex root extract 1:1 increases
Effect acts on most strong, co-toxicity coefficient 261.03；When ratio is 4:1,3:1,2:1,1:2,1:3,1:4, co-toxicity coefficient is followed successively by
194.39、213.62、232.67、227.34、201.00、186.07。
8.3 embodiments 2,4,5,7 prevent and treat Wolfberry Aphid field control effectiveness test:
Test is divided into two batches progress, and setting embodiment 2 and embodiment 5 respectively is first group, and embodiment 4 and embodiment 7 are the
Two groups, wherein the control sample of embodiment 2 is 6% root bark of celastrus angulatus extract and 2% Chinese ilex root extract, the control sample of embodiment 5
It is 3% root bark of celastrus angulatus extract and 6% Chinese ilex root extract, the control sample of embodiment 4 is 5% root bark of celastrus angulatus extract and 5%
Chinese ilex root extract, the control sample of embodiment 7 are 3% root bark of celastrus angulatus extract and 12% Chinese ilex root extract, while with clear water
For blank control.Wherein the specific preparation method of each control sample is referring to its corresponding each embodiment, unlike, by each embodiment
In active constituent replace with the active constituent of corresponding control sample, such as the control sample of embodiment 2 is respectively 6% Celastrus angulatus root
Bark extract and 2% Chinese ilex root extract, 6% root bark of celastrus angulatus extract of control sample are then that the active constituent in embodiment 2 is bitter
Skin rattan root bark extract and Chinese ilex root extract replace with root bark of celastrus angulatus extract, and other components are constant, and water supplement makes activity
Ingredient root bark of celastrus angulatus extractive content is 6%.
Triennial fructus lycii seedling is selected, is sprayed in Wolfberry Aphid 1st generation young age nymph beginning peak period with Backpack type mechanical mist and powder spraying machine
It applies.Every processing is repeated 3 times, the arrangement of completely random district's groups, and sets protection row.50 kg water/667 m2, after selecting fine day 16:00,
Configured medicament implementation complete stool is sprayed, emphasis sprays tree crown taper and leave dual sides, accomplish it is uniform, careful, cover comprehensively
Lid.Every cell selects 10 plants of trees, and 2 branches for selecting aphid more in each tree are marked with label as fixed sample branch, infused
Bright number, medicament title, concentration.The insect population on fixed sample branch is investigated before application.3 d, 7 d, 15 d difference after spray
Investigate work aphid number (Guo Rui, Yan Lin, Jin Shengying, the Chen Shengcui on the fixed sample branch of every cell.DS enable breathe out Wolfberry Aphid toxicity test and
Field control effectiveness test.Northern gardening, 2012, (10): 148-151).
Data analysis is carried out using SPSS22.0 software.It the results are shown in Table shown in 3 and table 4.
Control efficiency is the data by efficacy survey compared with blank district, is calculated control efficiency (Revision insect recluced rate),
Calculation formula is as follows:
。
3 first groups of embodiments of table and control group preparation prevent and treat Wolfberry Aphid field control effectiveness test
。
4 second groups of embodiments of table and control group preparation prevent and treat Wolfberry Aphid field control effectiveness test
。
By table 3 and table 4 it is found that the preparation of two kinds of group of effective components compounding of the invention obviously proposes Wolfberry Aphid control efficiency
It is high, hence it is evident that better than two kinds group of effective components are used alone, and have preferable quick-acting.Treatment agent is 8%, 9%, 10% and 15%
The extension rate of 500 times of bitter Chinese ilex, preventive effect is 76%~97%；In root bark of celastrus angulatus extract and Chinese ilex root extract ratio
When for 1:1 (embodiment 4), preventive effect is best, 3 d, 7 d, 15 d after processing, and preventive effect is up to 97.67%, 98.43% and respectively
98.42%, and to Target crops and beneficial organism safety in this test scope of medication.
8.4 embodiments 2,4,5,7 prevent and treat Paratrioza sinica field control effectiveness test:
Test is divided into two batches progress, and setting embodiment 2 and embodiment 5 respectively is first group, and embodiment 4 and embodiment 7 are the
Two groups.And the control sample of each embodiment is prepared respectively, wherein the control sample of embodiment 2 is 6% root bark of celastrus angulatus extract and 2% winter
Green root extract, the control sample of embodiment 5 are 3% root bark of celastrus angulatus extract and 6% Chinese ilex root extract, the control of embodiment 4
Sample is 5% root bark of celastrus angulatus extract and 5% Chinese ilex root extract, the control sample of embodiment 7 be 3% root bark of celastrus angulatus extract and
12% Chinese ilex root extract.Simultaneously using clear water as blank control.The preparation method of each control sample is the same as 8.3.
It tests and is carried out in July, 2014 in lycium barbarum production base.Every kind of medicament sets up 2 concentration processing separately, with clear water
For control.3 plants of trees are chosen in every processing, and random district's groups arrangement is repeated 3 times, with hand-powered knapsack sprayer that each plant is abundant
Spray is advisable up to No drip type.Check Paratrioza sinica radix is adjusted before application, respectively listing mark.3 d, 5 d, 7 d after application
The wood louse number on every is investigated, Paratrioza sinica Revision insect recluced rate and control efficiency (Chen Peimin, Wang Jianping, Zhong Yanping, Su Hui are calculated
It is bright, tension army.Several plant source chemical control Paratrioza sinica pharmacodynamic test.Inner Mongol agricultural science and technology, 2013(5): 66-67).
5 first groups of embodiments of table and control sample preparation prevent and treat Paratrioza sinica field efficacy
。
6 second groups of embodiments of table and control sample preparation prevent and treat Paratrioza sinica field efficacy
。
By table 5 and table 6 it is found that the preparation of two kinds of group of effective components compounding of the invention obviously proposes Paratrioza sinica control efficiency
It is high, hence it is evident that better than two kinds group of effective components are used alone, and have preferable quick-acting.Treatment agent is 8%, 9%, 10% and 12%
The extension rate of 500 times of bitter Chinese ilex, the preventive effect of 3 d is 71%~98%；It is extracted in root bark of celastrus angulatus extract and Chinese ilex root
Object ratio be 1:1 when (embodiment 4), preventive effect is best, 3 d, 7 d, 15 d after processing, preventive effect respectively be up to 98.64%,
97.96% and 98.29%.
In addition, we carry out root bark of celastrus angulatus extract and the most significant proportion 1:1 of Chinese ilex root extract synergistic effect
The acute toxicity test of small white mouse, regression equation are Y=- 1.29+1.64x, acute oral LD50Value is 6834.07 mg/kg,
In micro- malicious range.
In conclusion Pesticidal combination of the invention, which has, enhances Wolfberry Aphid, Paratrioza sinica control efficiency, quick-acting
Advantage good, that lasting effect is long in addition to significant insecticidal effect, can effectively reduce compared with existing single formulation
Dispenser cost, slows down the generation of resistance, reduces preparation residual quantity, pollution-free to crop, safety is good, meets the peace of pesticidal preparations
Full property requirement.
Claims (6)
1. a kind of Pesticidal combination, which is characterized in that its active constituent is root bark of celastrus angulatus extract and Chinese ilex root extract；It is bitter
The weight proportion of skin rattan root bark extract and Chinese ilex root extract is 1~4:1~4；The root bark of celastrus angulatus extract via with
Lower step obtains: root bark of celastrus angulatus crushed, root bark of celastrus angulatus powder is obtained, the ethyl alcohol of 3~5 times of root bark of celastrus angulatus powder weight is added,
In 60~80 DEG C of 1~4h of ultrasonic extraction, it is concentrated under reduced pressure after filtering to obtain the final product；The Chinese ilex root extract is obtained via following steps
: Chinese ilex root is crushed, the ethyl acetate of 3~6 times of Chinese ilex root powder weight is added, in 50~80 DEG C of 3~5h of ultrasonic extraction, filtering
After be concentrated under reduced pressure to obtain the final product.
2. Pesticidal combination according to claim 1, which is characterized in that the Pesticidal combination is aqueous emulsion, weight
Percentage composition are as follows: active constituent 5~15%, cyclohexanone 15~30%, sodium benzoate 0.5~1.2%, ethylene glycol 0.5~1.2%,
1,2-butanediol 0.5~1.5%, agriculture breast 2201 are 3~5%, remaining is water.
3. Pesticidal combination according to claim 2, which is characterized in that the weight percent of the aqueous emulsion forms are as follows:
Root bark of celastrus angulatus extract 5%, Chinese ilex root extract 5%, cyclohexanone 30%, sodium benzoate 0.9%, ethylene glycol 0.8%, 1,2-
Butanediol is 1.2%, agriculture breast 2201 is 4.2%, remaining is water.
4. the preparation method of any Pesticidal combination of claim 1-3, which comprises the following steps:
1) root bark of celastrus angulatus extract, Chinese ilex root extract are mixed into obtain oily phase with cyclohexanone；
2) by agriculture breast 2201, sodium benzoate, ethylene glycol, 1, the 1/2 of 2-butanediols and water consumption mixes to obtain water phase；
3) water phase is added in oily phase and is mixed, while adding remaining water to get aqueous emulsion.
5. the preparation method of Pesticidal combination as claimed in claim 4, which is characterized in that when being mixed in step 1) and step 2
10~30 min are stirred with the speed of 800~1000 r/min；In the height of 10000~12000 r/min when step 3) mixes
Speed is lower to stir 10~30min, while supplementing the water of surplus.
6. the application of any Pesticidal combination of claim 1-3, which is characterized in that be applied to Lycium Pests
Prevention and treatment.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1086961A (en) * | 1992-11-19 | 1994-05-25 | 西北农业大学 | Celastrus angulatus emulsified oil as plant's pesticide and manufacture method thereof |
| CN1237349A (en) * | 1999-06-25 | 1999-12-08 | 西北农业大学 | Two kinds of celastrus angulatus water base insecticidal preparation |
| CN103315006A (en) * | 2013-04-22 | 2013-09-25 | 河南省科高植物天然产物开发工程技术有限公司 | Insecticidal composition containing natural celastrus angulatus and natural vitex negundo and application thereof |
| CN103329943A (en) * | 2013-04-22 | 2013-10-02 | 河南省科高植物天然产物开发工程技术有限公司 | Insecticide composition containing natural celastrus angulatus and natural pyrethrin |
-
2016
- 2016-12-09 CN CN201611128510.2A patent/CN106665665B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1086961A (en) * | 1992-11-19 | 1994-05-25 | 西北农业大学 | Celastrus angulatus emulsified oil as plant's pesticide and manufacture method thereof |
| CN1237349A (en) * | 1999-06-25 | 1999-12-08 | 西北农业大学 | Two kinds of celastrus angulatus water base insecticidal preparation |
| CN103315006A (en) * | 2013-04-22 | 2013-09-25 | 河南省科高植物天然产物开发工程技术有限公司 | Insecticidal composition containing natural celastrus angulatus and natural vitex negundo and application thereof |
| CN103329943A (en) * | 2013-04-22 | 2013-10-02 | 河南省科高植物天然产物开发工程技术有限公司 | Insecticide composition containing natural celastrus angulatus and natural pyrethrin |
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