CN101796968B - Method for preparing preparation for preventing plant aphids by utilizing tomato stem and leaf extract - Google Patents
Method for preparing preparation for preventing plant aphids by utilizing tomato stem and leaf extract Download PDFInfo
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- CN101796968B CN101796968B CN 201010120582 CN201010120582A CN101796968B CN 101796968 B CN101796968 B CN 101796968B CN 201010120582 CN201010120582 CN 201010120582 CN 201010120582 A CN201010120582 A CN 201010120582A CN 101796968 B CN101796968 B CN 101796968B
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Abstract
The invention relates to a method for preparing preparation for preventing plant aphids by utilizing tomato stem and leaf extract in the technical field of pesticide preparation. The preparation comprises the following components in percentage by mass: 0.1 to 3 percent of tomato stem and leaf extract and the balance of water. After the preparation prepared by the method is used for 24 hours, the death rate of aphids can reach 99 percent.
Description
Technical field
What the present invention relates to is preparation of a kind of agricultural fungicides technical field and preparation method thereof, specifically a kind of method of utilizing tomato stem-leaf extract to prepare control plant aphids preparation.
Background technology
Aphis Homoptera, Aphidoidea, Known Species is nearly 4000 kinds in the world.Aphid is the major pest of plant, sucks water, not only hinders plant growth, forms insect gall, and cause flower, leaf, bud deformity, and affect the photosynthesis of blade, cause the underproduction, the more important thing is and to propagate the various vegetables virus disease, result in greater loss, when serious even can have no harvest.The chemical pesticides such as people's normal operation Aphox and Imidacloprid come aphid is prevented and treated at present.But use in a large number chemical pesticide can impel insect to develop immunity to drugs, residue of pesticide can damage and pollute food chain and environment.Therefore, safety, efficient plant insecticide will become main developing direction, have wide future develop.
Tomato (Solanum lycopersicun) belongs to plant of Solanaceae, and wide geographic area has plantation in China, aboundresources; Found that at present tomato stem-leaf extract has inhibitory action to more than 30 kinds of fungies, and it is less to the report of its insecticidal action, its cauline leaf causes waste as agricultural wastes always, the present invention will utilize tomato stem-leaf to carry out the preparation of botanical pesticide, not only can turn waste into wealth, can also reach the purpose of safe and efficient control aphid.
Through the prior art literature search is found, Chinese patent literature CN101233865A has put down in writing a kind of " matrine preparation vegetable insecticide of special for killing aphids and preparation method thereof ", mention a kind of green plant pesticide that is formed by various plants such as kuh-seng, China tree, tarragon, Celastrus angulatus, trifoliate jewelvines in this technology, aphid had preferably control efficiency, but it is longer that it reaches high preventive effect required time, and its composition is too many, and relative cost is higher.
Summary of the invention
The present invention is directed to the prior art above shortcomings, a kind of method of utilizing tomato stem-leaf extract to prepare control plant aphids preparation is provided, make it have higher control efficiency to aphid, can be directly used in the insect pest of this kind of control, and have nuisanceless, pollution-free and without the characteristics of residual hazard, avoid the harm of chemical pesticide.
The present invention is achieved by the following technical solutions,
The present invention relates to a kind of control plant aphids preparation that utilizes tomato stem-leaf extract, its component and mass percentage content thereof are: tomato stem-leaf extract 0.1-3%, surplus is water.
The present invention relates to the above-mentioned preparation method who utilizes the control plant aphids preparation of tomato stem-leaf extract, may further comprise the steps:
The first step, preparation tomato stem-leaf ethanol crude extract: at first gather the fresh cauline leaf of tomato and heating, drying, add after crushed lytic agent and after adding thermal agitation cold filtration, make the tomato stem-leaf ethanol crude extract;
Described heating, drying refers to the fresh cauline leaf of tomato placed under 40-100 ℃ the environment and dries;
Described lytic agent refers to: mass percent concentration is the ethanol of 25%-100%, and the consumption of this lytic agent is 5-20 times of the fresh cauline leaf weight of tomato after pulverizing;
Describedly add thermal agitation and refer to: under 80-100 ℃ of temperature, stir 15-30min.
Second step, employing reduced pressure concentration method are made tomato stem-leaf medicinal extract with the tomato stem-leaf ethanol crude extract.
Described reduced pressure concentration method refers to the tomato stem-leaf ethanol crude extract pumped to be pressed onto-0.1 atmospheric vacuum state and to place carry out water-bath under 80-100 ℃, the concentrated tomato stem-leaf medicinal extract that obtains.
The 3rd step, tomato stem-leaf medicinal extract adding distil water dilution is obtained insect protected suspension by ultrasonic dispersion, obtain tomato stem-leaf extract through being evaporated to after the organic matter extraction after doing again, make plant aphids control preparation through adding aqueous suspension.
The mass ratio of tomato stem-leaf medicinal extract and distilled water is in the described insect protected suspension: 1: 33 ~ 1000.
Described organic matter extraction refers to: adopt with the isopyknic benzinum of insect protected suspension, ethyl acetate, n-butanol respectively respectively to extract five times;
Describedly add aqueous suspension and refer to: 33 ~ 1000 times the water and its mixing and stirring that adopt the tomato stem-leaf extract quality.
The present invention has the following advantages compared with the prior art: this insecticide derives from the stem-leaf extract of cultivated plant, can be processed into wetting powder, suspending agent or water dispersant.Its environmentally safe to people, animal safety, is a kind of pollution-free plant source biological insecticides; This insecticide is that wide, the resourceful agricultural wastes tomato stem-leaf that distributes is raw material, and is with low cost, turns waste into wealth, and is conducive to realize the development of sustainable agriculture; And its extraction process is simple, need not special instrument, and the solvent after the recovery also can reuse; Said preparation has good control efficiency to aphid.
Embodiment
The below elaborates to embodiments of the invention, and present embodiment is implemented under take technical solution of the present invention as prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
Control aphid weight of formulation percentage: tomato stem-leaf extract 1%, distilled water 99%.
Take by weighing tomato stem-leaf powder (30 order particle) 100g, ethanolic solution 1000ml with 70% is at the indoor 15min that boils, filter, get its filtrate decompression concentration and recovery solvent and obtain extracting medicinal extract, add 2000ml distilled water, place supersonic generator 15min that dry matter is suspended, be mixed with concentration and be 1% preparation; Adopt indoor blade method to give birth to survey to aphid, with the cotton, the gauze that soak green vegetables blade stem end is pinioned, connect vegetable aphid, then spray medicine, place culture dish to cultivate, statistics aphid lethality after 24 hours, its preventive effect is 44.7%.
Embodiment 2
Take by weighing tomato stem-leaf powder (30 order particle) 100g, ethanolic solution 1000ml with 70% is at the indoor 15min that boils, filter, get its filtrate decompression concentration and recovery solvent and obtain extracting medicinal extract, add 1000ml distilled water, place supersonic generator 15min that dry matter is suspended, be mixed with concentration and be 2% preparation.Respectively extract five times with isopyknic benzinum, ethyl acetate, n-butanol successively respectively again; Respectively will be wherein water slurry after benzinum, ethyl acetate, butanol extraction liquid and the extraction be evaporated to dried, namely get tomato stem-leaf extract, be made into 2% preparation with deionized water or dissolved in distilled water, adopt indoor blade method to give birth to survey to vegetable aphid, statistics aphid lethality after 24 hours.Benzinum phase and water do not have control efficiency to aphid; The preventive effect of ethyl acetate phase and the relative aphid of n-butanol is respectively 73.7% and 99%.
Embodiment 3
Control aphid weight of formulation percentage: tomato stem-leaf extract 2%, water: 98%.
Take by weighing tomato stem-leaf powder (40 order particle) 100g, ethanolic solution 1000ml with 75% is at the indoor 20min that boils, filter, get its filtrate decompression concentration and recovery solvent and obtain extracting medicinal extract, add 1000ml distilled water, place supersonic generator 15min that dry matter is suspended, be mixed with concentration and be 2% preparation; Adopt the indoor pot method to give birth to survey to vegetable aphid, add up lethality after 24 hours, its preventive effect is 97.5%.
Embodiment 4
Control aphid weight of formulation percentage: tomato stem-leaf extract 2%, water: 98%.
Take by weighing tomato stem-leaf powder (40 order particle) 100g, ethanolic solution 1000ml with 75% is at the indoor 20min that boils, filter, get its filtrate decompression concentration and recovery solvent and obtain extracting medicinal extract, add 1000ml distilled water, place supersonic generator 15min that dry matter is suspended, be mixed with concentration and be 2% preparation; Adopt the indoor pot method to give birth to survey to the cucumber cotten aphid, add up lethality after 24 hours, its preventive effect is 67.5%.
Embodiment 5
Control aphid weight of formulation percentage: tomato stem-leaf extract 3%, water: 97%.
Take by weighing tomato stem-leaf powder (50 order particle) 100g, ethanolic solution 2000ml with 80% is at the indoor 30min that boils, filter, get its filtrate decompression concentration and recovery solvent and obtain extracting medicinal extract, add 800ml distilled water, place supersonic generator 15min that dry matter is suspended, be mixed with concentration and be 3% preparation; Adopt the experiment of residential quarter, field vegetable aphid is carried out field control effectiveness test, add up lethality after 24 hours, preventive effect reaches 96%.
Claims (1)
1. a preparation method who prevents the plant aphids preparation who utilizes tomato stem-leaf extract is characterized in that, its component of said preparation and mass percentage content thereof are: tomato stem-leaf extract 0.1-3%, and surplus is water, said method comprising the steps of:
The first step, preparation tomato stem-leaf ethanol crude extract: at first gather the fresh cauline leaf of tomato and the fresh cauline leaf of tomato placed under 40-100 ℃ the environment and dry, add after crushed consumption for the 5-20 mass percent concentration doubly of the fresh cauline leaf weight of tomato after pulverizing be 70% ethanol and after stirring 15-30min under the 80-100 ℃ of temperature cold filtration, make the tomato stem-leaf ethanol crude extract;
Second step, the tomato stem-leaf ethanol crude extract pumped to be pressed onto-0.1 atmospheric vacuum state and to place carry out water-bath under 80-100 ℃, the concentrated tomato stem-leaf medicinal extract that obtains;
The 3rd step, tomato stem-leaf medicinal extract diluted by 1: 33～1000 ratio adding distil water obtain insect protected suspension by ultrasonic dispersion, adopt respectively again to be evaporated to after respectively extracting five times with the isopyknic benzinum of insect protected suspension, ethyl acetate, n-butanol to obtain tomato stem-leaf extract after doing, make plant aphids control preparation by 33～1000 times water mixing and stirring dissolving of tomato stem-leaf extract quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010120582 CN101796968B (en) | 2010-03-09 | 2010-03-09 | Method for preparing preparation for preventing plant aphids by utilizing tomato stem and leaf extract |
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| CN 201010120582 CN101796968B (en) | 2010-03-09 | 2010-03-09 | Method for preparing preparation for preventing plant aphids by utilizing tomato stem and leaf extract |
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| CN101796968A CN101796968A (en) | 2010-08-11 |
| CN101796968B true CN101796968B (en) | 2013-05-01 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907402A (en) * | 2012-10-26 | 2013-02-06 | 扬州绿源生物化工有限公司 | Insect pest trap |
| CN103478180A (en) * | 2013-07-02 | 2014-01-01 | 江苏希旺农业科技有限公司 | Pesticide for organic vegetables |
| CN104509416A (en) * | 2015-01-14 | 2015-04-15 | 蒋君军 | Method for preventing and treating tomato diseases and insect pests |
| CN104996500A (en) * | 2015-06-23 | 2015-10-28 | 上海交通大学 | Compound plant pesticide capable of controlling plant aphid, and preparation and application thereof |
| CN105028536B (en) * | 2015-07-07 | 2018-07-03 | 上海交通大学 | It is a kind of for the compound plant insecticide of mosquito larvae control and its preparation and application |
| CN113854305A (en) * | 2021-11-11 | 2021-12-31 | 华植河北生物科技有限公司 | Insecticidal composition containing pyriproxyfen and dinotefuran |
| CN115413682B (en) * | 2022-08-29 | 2024-04-05 | 山东京博农化科技股份有限公司 | Application of tomato stem and leaf extract in preventing and treating thrips and composition |
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