CN105746610B - A kind of synergist of plant source aphicide - Google Patents
A kind of synergist of plant source aphicide Download PDFInfo
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- CN105746610B CN105746610B CN201610170300.3A CN201610170300A CN105746610B CN 105746610 B CN105746610 B CN 105746610B CN 201610170300 A CN201610170300 A CN 201610170300A CN 105746610 B CN105746610 B CN 105746610B
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- robinia pseudoacacia
- bamboo vinegar
- pseudoacacia seeds
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- 238000005516 engineering process Methods 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
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- CLEXYFLHGFJONT-UHFFFAOYSA-N jervine Natural products C1C=C2CC(O)CCC2(C)C(C(=O)C2=C3C)C1C2CCC13OC2CC(C)CNC2C1C CLEXYFLHGFJONT-UHFFFAOYSA-N 0.000 description 1
- QRXOCOSLDOPPKH-UHFFFAOYSA-N jervine sulfate Natural products CC1CNC2C(C1)OC3(CCC4=C(C3C)C(=O)C5C4CC=C6CC(O)CCC56C)C2C QRXOCOSLDOPPKH-UHFFFAOYSA-N 0.000 description 1
- -1 ketone and esters Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
A kind of synergist of plant source aphicide, belongs to aphicide technical field.Bamboo vinegar has synergistic effect to the plant source aphicide of the extract containing Robinia pseudoacacia Seeds, can be used as synergist.Botanical pesticide is compared with chemical pesticide there are the features such as drug effect is slow, activity is low, and therefore, addition pesticide synergistic agent is one of main method for improving botanical pesticide drug effect.Present invention demonstrates that bamboo vinegar dilutes 100 400 times of solution has synergistic effect, and pass through Toxicity Determination and field efficacy experimental verification to the plant source aphicide based on Robinia pseudoacacia Seeds extract.The plant source aphicide and bamboo vinegar synergist of independent research derive from vegetable material, have the characteristics that degradable, pollution-free and with nuisanceless, safe and efficient, can be that the agricultural product of green, the organic contour attached value of fruits and vegetables of production provide novel effective solution.
Description
Technical field
The invention belongs to aphicide technical fields, and in particular to a kind of synergist of plant source aphicide.
Background technology
Bamboo vinegar be it is a kind of using bamboo processing waste material as raw material carry out pyrolysis formation one kind contain acids, phenols, aldehydes,
The compounds such as ketone and esters, and the brown liquid with smoke.Documents and materials access is shown：Bamboo vinegar has itself
Coordinate plant growth, antibacterial, desinsection improve the effect of soil fertility etc..Bamboo vinegar is widely used in chemical product, protects
The fields such as strong product, feed, beverage, medicine and agricultural.At present, have many scholars and carried out itself and different drugs to bamboo vinegar
Synergistic function research.But further deeply retrieval shows：There is presently no bamboo vinegars and the plant source of independent research
Aphicide mixture is published using or as the patent that uses of synergist of plant source aphicide.
Aphid Myzus persicae (Sulzer) belong to Semiptera Hemiptera, Aphidiadae Aphididae.It is alias aphid, greasy
Worm.Aphid is that one of most strong, most destructive pest of most fast, fertility is bred on the earth, is in agricultural and production of forestry
Endanger one of mostly important pest.The control of aphid is mostly still used based on chemical pesticide control at present, however long-term
The use of chemical pesticide so that aphid produces resistance and certain killing is produced to natural enemy and beneficial organism.Chemical pesticide
Abuse, do not met people and pursued environmentally protective demand, therefore, the plant source aphicide for researching and developing new type of safe substitutes chemistry
Pesticide is one of developing direction.However, compared with chemical pesticide, botanical pesticide there are drug effect it is slow the deficiencies of.Therefore, by
It is one of main method to solve the above problems that suitable synergist is added in plant insecticide.
Locust tree, scientific name Robinia pseudoacacia L.Also known as acacia.Belong to Angiospermae, Rosales, pulse family, butterfly
Shape spends subfamily deciduous tree.Because it is with stronger adaptability and fast-growing character, now plant extensively all over China.Research knot is reported
Fruit shows to mainly contain the ingredients such as flavonoids, glycoside, protein and its Polyphenols in locust tree.It is wide with multiple biological activities
It is general to apply in the fields such as medicine and agricultural.
Through showing that at present the research that locust tree is used as pesticide plant is less to existing documents and materials access.For radiance etc.
Chinese patent (CN102687733A) is research shows that locust tree branch, stem, leaf, flower extract live to Tetranychus cinnabarinus with good mite killing
Property.Liu Hongxia etc. exists《Forestry scientific research》(2 phases in 2004) entitled " Seed Lectins from Robinia pseudoacacia on Metabolism of Lymantria dispar Larvae
The Primary Study of influence " shows that Robinia pseudoacacia Seeds agglutinin has certain toxic action to Lymantria dispar larvae.Further retrieve table
It is bright, have no the research report of Robinia pseudoacacia Seeds killing aphids activity, while also have no that Robinia pseudoacacia Seeds extract is used as botanical pesticide
Patent discloses.
Invention content
The present invention, which provides a kind of plant source aphicide to the extract containing Robinia pseudoacacia Seeds, the substance i.e. bamboo vinegar of synergistic effect
Liquid.
The preparation of Robinia pseudoacacia Seeds ethanol extract：After Robinia pseudoacacia Seeds naturally dry is crushed.4-20 times of weight is added in thorn
The ethanol-water solution room temperature extraction of Chinese scholartree seed powder, extracting solution filtering collect, merge and be concentrated under reduced pressure into medicinal extract to get to locust tree
Seed alcohol extracts medicinal extract；Robinia pseudoacacia Seeds alcohol extracts are put into -80 DEG C of freeze drier and are lyophilized to get to locust tree
Seed ethanol extract dried powder；Ethyl alcohol:Water volume ratio 1:(0-1).
The new opplication of bamboo vinegar：Bamboo vinegar kills aphid synergist as Robinia pseudoacacia Seeds ethanol extract.
Have present invention demonstrates that bamboo vinegar dilutes 100-400 times of solution to the plant source aphicide based on Robinia pseudoacacia Seeds extract
There is synergistic effect, and pass through Toxicity Determination and field efficacy experimental verification.Plant source aphicide and bamboo vinegar synergist are equal
From vegetable material, have the characteristics that degradable, pollution-free and with nuisanceless, safe and efficient, can be production green, have
The agricultural product of the contour attached value of machine fruits and vegetables provide novel effective solution.
Specific embodiment
With reference to embodiment, the present invention will be further described, but the present invention is not limited to following embodiments.
For trying insect
Cotten aphid (Aphis gossypii Glover) in August, 2013 is collected in Hebei province Langfang suburbs cotton field, with interior
The cotton seedling breeding number generation that Holland nutrient solutions are cultivated.
For the preparation of reagent liquid
10% Robinia pseudoacacia Seeds extract aqueous emulsion：Accurately weigh 10g (mass percentage 10%) Robinia pseudoacacia Seeds extract
Freeze-dried powder is dissolved in solvent, in SYNPERONIC PE/F68, the sorbitan fatty acid ester ring for adding in 5-15%
Oxidative ethane addition product and fatty alcohol polyoxyethylene ether form uniform oil phase as emulsifier.The third the three of 3% will be added in water again
Alcohol as antifreeze, 1% polyethylene glycol as stabilizer, 1% organosilicon for antifoaming agent, 0.5% aluminum magnesium silicate,
0.1% xanthans mixing forms water phase.Water phase is added in oil phase under the high-speed shearing machine stirring of 4000r/min, it is high
Fast emulsification pretreatment 10min, forms well dispersed aqueous emulsion.
Bamboo vinegar of the present invention refers to the by-product that the high temperature pyrolysis during bamboo processing generates and by not
With the refined products for the different phase that stage rectifying is prepared.
Robinia pseudoacacia Seeds ethanol extract accurately is weighed, with acetone accurate formulation into the liquid of 125mg/L；Accurate measure is fitted again
The bamboo vinegar of amount, and with acetone gradient dilution into 200 times of 100 times of dilutions (BV100) of bamboo vinegar, bamboo vinegar dilutions
(BV200), 400 times of dilutions (BV400) of bamboo vinegar；Then 3 kinds of Robinia pseudoacacia Seeds ethanol extracts and bamboo vinegar are prepared with acetone
Mixed mark solution, respectively Robinia pseudoacacia Seeds ethanol extract 125mg/L and 100 times of dilution mixture liquid (125mg/L+ of bamboo vinegar
BV100, i.e., a concentration of 125mg/L of Robinia pseudoacacia Seeds extract, the multiple that bamboo vinegar is diluted in the solution in admixing medical solutions
It is 100, solvent is acetone)；Robinia pseudoacacia Seeds ethanol extract 125mg/L and 200 times of dilution mixture liquid (125mg/ of bamboo vinegar
L+BV200, i.e., a concentration of 125mg/L of Robinia pseudoacacia Seeds extract in admixing medical solutions, times that bamboo vinegar is diluted in the solution
Number is 200, and solvent is acetone)；Robinia pseudoacacia Seeds ethanol extract 125mg/L and 400 times of dilution mixture liquids of bamboo vinegar
(125mg/L+BV400, i.e., a concentration of 125mg/L of Robinia pseudoacacia Seeds extract, bamboo vinegar are dilute in the solution in admixing medical solutions
The multiple released is 400, and solvent is acetone).The experiment of aphid indoor bioassay is done to above-mentioned 7 kinds of liquids.
10% Robinia pseudoacacia Seeds extract aqueous emulsion sets 2 dosage gradients：That is 400mL/ mus (EW400) and 600mL/
Mu (EW600)；200 times of dilutions of bamboo vinegar/mu dosage (BV200), 400 times of dilutions of bamboo vinegar/mu dosage (BV400)；It sets simultaneously
Put 0.5% 80mL/ mus of black false hellebore alkali-soluble liquor of plant source comparison medicament.10% Robinia pseudoacacia Seeds extract aqueous emulsion 400mL/
Mu (EW400) and 200 times of dilutions of bamboo vinegar/mu dosage (BV200) mixture liquid ((locust tree aqueous emulsion 400ml:Raw liquid of bamboo vinegar
125ml=16:5, v/v), i.e. EW400+BV200；10% Robinia pseudoacacia Seeds extract aqueous emulsion 400mL/ mus (EW400) and bamboo vinegar
400 times of dilutions of liquid/mu dosage (BV400) mixture liquid (locust tree aqueous emulsion 400ml:Raw liquid of bamboo vinegar 62.5ml=32:5, v/
V), i.e. EW400+BV400.
Embodiment 1
Indoor bioassay method
Contact toxicity is tested --- drop method, according to People's Republic of China's agricultural industry criteria (NY/T 1154.1-
2006) insecticide part 1-drop method standard is implemented.The medicine for the Robinia pseudoacacia Seeds ethanol extract 125mg/L that above-mentioned acetone is prepared
400 times of liquid, 100 times of dilutions (BV100) of bamboo vinegar, 200 times of dilutions (BV200) of bamboo vinegar, bamboo vinegar dilutions (BV400)
And the mixed liquid Robinia pseudoacacia Seeds ethanol extract 125mg/L of the two and 100 times of dilution mixture liquid (125mg/L+ of bamboo vinegar
BV100)；Robinia pseudoacacia Seeds ethanol extract 125mg/L and 200 times of dilution mixture liquids (125mg/L+BV200) of bamboo vinegar；Thorn
Chinese scholartree seed ethanol extract 125mg/L and 400 times of dilution mixture liquids (125mg/L+BV400) of bamboo vinegar totally 7 kinds of liquids pair
Cotten aphid makees indoor contact toxicity bioassay.
The cotten aphid of artificial feeding on indoor water planting cotton seedling is chosen, worm age, larvae aphid of the same size are gently chosen with writing brush, as
Worm is tried out in confession in activity test, and solution to be measured is drawn with micro syringe, will be for reagent liquid drop with automatic fine intravenous drip instrument
On the pronotary of aphid, and the acetone without medicament is set as blank control.Ensure each processing drop 150 with
On, 3 repetitions.Encase cotton petiole with wet cotton after processing and be put into moisturizing in culture dish, be placed in illumination box, for 24 hours after
Microscopy is carried out under magnifying glass, records dead borer population, and corrected mortality is calculated with Abbott formula.
Calculation formula：
1 Robinia pseudoacacia Seeds ethanol extract of table and synergistic activity in bamboo vinegar room
Table 1 synergistic activity for vinegar and ethanol extract of
R.pseudoacacia Complex against Aphis gossypii Glover
Above-mentioned 7 medicaments the results are shown in Table 1 to the indoor bioassay of cotten aphid.As known from Table 1：Bamboo vinegar is carried with Robinia pseudoacacia Seeds
Object is taken to be respectively provided with certain killing aphids activity, and 3 kinds of Mixed Pharmacies are significantly higher than single dose to the contact toxicity of cotten aphid for 24 hours
Bioactivity, this illustrates that bamboo vinegar can be used as a kind of suitable synergist and Robinia pseudoacacia Seeds extract aqueous emulsion to compound, system
Into the plant source aphicide of relative efficiency.
Embodiment 2
Field efficacy experimental method
According to People's Republic of China's agricultural industry criteria, NY/T 1464.27-2010 standards perform.
Experimental subjects
Aphid, experiment crop are cabbage type rape.
The selection in experimental plot
Experimental field is City of Wuhu in Anhui Fanchang County Rapeseed Field, selects soil fertility consistent, and aphid generation is more serious, and
The consistent field of the extent of injury is tested.
Experiment packet
10% Robinia pseudoacacia Seeds extract aqueous emulsion sets 2 dosage gradients：That is 400mL/ mus and 600mL/ mus；Bamboo vinegar
200 times of dilutions of liquid/mu dosage, 400 times of dilutions of bamboo vinegar/mu dosage.Set 0.5% jervine of plant source comparison medicament can simultaneously
80mL/ mus of dissolubility liquor.And clear water control, each processing are repeated 3 times.Hillslope processes area is 20m2, using random groups
Arrangement is closed, experimental field is surrounded by protection zone, and minizone is equipped with protection and goes.
Preventive effect is investigated
Using 5 points of samplings, every is investigated 3 plants, and every plant of label one opens the blade for having enough Aphed populations, and aphid is investigated per cell
Borer population is not less than 500.Before dispenser, insect population is investigated, dispenser respectively investigates aphid number living after 1,3,7d.
Preventive effect calculates
2 medicament of table is to the field efficacy of aphid
Table 2 The efficacy of agent against aphids in Field
Field efficacy experimental result is shown in Table 2.As known from Table 2：Two kinds of applied agents of 10% Robinia pseudoacacia Seeds extract aqueous emulsion
Amount (400 and 600mL/ mus) shows aphid good field efficacy, and preventive effect gradually increases in dispenser 7 days.Together
When it is found that 2 kinds of Mixed Pharmacies show certain synergistic effect on the whole to the preventive effect of aphid compared with single dose.After dispenser 1 day
Begin to show certain synergistic effect, preventive effect is respectively 64.33% and 55.24%, the list of significantly higher than similary dosage
Agent (43.23%).After medicine 3 days 2 kinds of Mixed Pharmacies to the preventive effect (88.72% and 81.43%) of aphid just with high dose (600mL/
Mu) single dose preventive effect (86.64%) quite, illustrate in reagent agent add bamboo vinegar can not only reach good preventive effect, also
The dosage of medicament can be reduced.These are the result shows that bamboo vinegar can become a kind of pollution-free plant source synergy for having very much a potentiality
Agent has wide application value.
Claims (3)
1. the new application of bamboo vinegar：Bamboo vinegar kills aphid synergist as Robinia pseudoacacia Seeds ethanol extract；Robinia pseudoacacia Seeds ethyl alcohol carries
Take the preparation of object：After Robinia pseudoacacia Seeds naturally dry is crushed, it is molten in the alcohol-water of Robinia pseudoacacia Seeds powder to add in 4-20 times of weight
Liquid room temperature extracts, and extracting solution filtering collects, merges and be concentrated under reduced pressure into medicinal extract to get to Robinia pseudoacacia Seeds alcohol extracts medicinal extract；It will
Robinia pseudoacacia Seeds alcohol extracts are put into -80 DEG C of freeze drier and are lyophilized to get to Robinia pseudoacacia Seeds ethanol extract xeraphium
End；Ethyl alcohol:Water volume ratio 1:（0-1）, the wherein volume of water is not 0.
2. the new application of the bamboo vinegar according to claim 1, which is characterized in that by Robinia pseudoacacia Seeds ethanol extract dried powder system
It is standby into 10 % Robinia pseudoacacia Seeds extract aqueous emulsions：Accurately weighing Robinia pseudoacacia Seeds ethanol extract dried powder is dissolved in organic solvent
In, add the SYNPERONIC PE/F68, sorbitan fatty acid ester ethylene oxide adduct and fat of 5-15%
Alcohol polyoxyethylene ether forms uniform oil phase as emulsifier；Again into water add in 3% glycerine as antifreeze, 1% gather
Ethylene glycol is antifoaming agent, 0.5% aluminum magnesium silicate, 0.1% xanthans mixing formation water phase as stabilizer, 1% organosilicon；
Water phase is added in oil phase under the high-speed shearing machine stirring of 4000r/min, it is good to form dispersion by high speed shearing emulsification 10min
Good aqueous emulsion；Mass percentage of the Robinia pseudoacacia Seeds ethanol extract dried powder in Robinia pseudoacacia Seeds extract aqueous emulsion
10%。
3. the new application of the bamboo vinegar according to claim 2, which is characterized in that by 10% Robinia pseudoacacia Seeds extract aqueous emulsion and bamboo
Vinegar liquid dilution matches.
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