CN102007944B - Plant source synergistic compound aphicide and acaricide containing rotenone and preparation method thereof - Google Patents
Plant source synergistic compound aphicide and acaricide containing rotenone and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a plant source synergistic compound aphicide and acaricide containing rotenone, and the prepared plant source synergistic compound acaricide is prepared by compounding Szechuan lovage rhizome oil and the rotenone as main active ingredients with auxiliaries, wherein the weight ratio of the rotenone to the Szechuan lovage rhizome oil is (8:2)-(7:3), and the preparation form can be oil emulsion, microemulsion and aqueous emulsion. The plant source synergistic compound aphicide and acaricide has higher control effect in fields for aphis citricola, cabbage aphid, macrosiphoniella sanborni, aphis gossypii glover and other aphids on fruit trees, vegetables and flowers, simultaneously has good control effect for tetranychus viennensis zacher, tetranychus cinnabarinus andother mite type pests, and has great application and popularization value on organic crops.
Description
Technical field
The invention belongs to biological pesticide technical field, relate in particular to a kind of take that cnidium oil and rotenone two Plants source materials are active component kill the aphid miticide.Be mainly used in the aphids such as control apple yellow aphid, cabbage aphid, the little Macrosiphus spp of chrysanthemum and matrimony vine aphid, and the evil mites such as tetranychus viennensis, Tetranychus cinnabarinus and Tetranychus urticae.
Background technology
Mite class (mites) belongs to Arthropoda (Arachinida), arachnids (Arachnida), Acari (Acari).According to its economics meaning, can be divided into the doctor and herd acarian and the large class of Agricultural Mites 2.Wherein important Agricultural Mites concentrates on 6 sections of Acariformes (Acariformes), and wherein most important harmful mite is tetranychid, is secondly time palpus mite, goitre Man, Fu line mite and flour mite etc.Wherein tetranychid is monoid maximum in Agricultural Mites, and 16 genus more than 200 that have that China is known are planted; The goitre mite of China's record has more than 180 to plant; Harm Yan Chong Fu line mite has Ce Duo Shi Fu line mite and Shui Dao Fu line mite, and the former endangers more than 30 sections more than 70 such as tealeaves, capsicum, eggplant and belongs to Plants, and latter is the important harmful mite on paddy rice.
Agricultural Mites has stronger diffusivity, is the kind of constantly occupying temporary habitat, of short duration living environment is had to the adaptability of height.Animal migration is the important component part of their population formation, and there is generation in even per generation.Individuality is little, fertility strong, offspring's lethality is high, life-span and each cycle from generation to generation are short, it is typical R class pest, they destroy the normal physiological function of plant, cause fallen leaves, bud drop, shedding, gently cause the underproduction, weaken tree vigo(u)r, heavy lose to receive or whole strain death, be one of pest of the most difficult control of generally acknowledging.
Aphid is another kind of important pest and to have a breeding fast, and quantity is large, and kind is many, the heavy and wide characteristics of damaging range of harm, and many aphids can also propagate the viroses of plant, and in control, difficulty is larger.
People aphid and evil mite prevent and treat process in excessive, frequently use non-specific chemical pesticide, the natural enemy of insect is killed in a large number, cause the vicious circle of " more anti-heavier; more heavily more anti-", " 3R " problem of chemical pesticide generally gets more and more people's extensive concerning, and people are also more and more deep to the understanding of agricultural chemicals.The use of agricultural chemicals is not focused on killing off the insect pests, and more focuses on regulating, and therefore, kills the inevitable biogenic pesticide future development towards efficient, low toxicity, wide spectrum, safety of the acaricidal development of aphid.
Botanical pesticide active ingredient safety, low toxicity, the Secondary Metabolism of Plant product that a class has insecticidal activity mostly, along with the proposition of concepts such as " public nuisance-free agricultural chemicals " and " environment harmonious agricultural chemicals " with deeply, requirement to agricultural chemicals is more and more harsher, for promoting agricultural sustainable development, plant resource is killed to the aphid miticide, and to carry out Research Significance great.
Cnidium oil be the samphire Ligusticum wallichii (
ligusticum chuanxionghort.) volatile oil of separating in dry rhizome, the main chemical compositions that wherein Ligustilide, dihydro manuscript lactone, cyclobutenyl Ligustilide are cnidium oil.Research shows that cnidium oil has the inhibition platelet activation, improves the blood microcirculation, reduces vascular resistence, and ischemic disease is had to significant curative effect.Simultaneously, also find that cnidium oil can make microcirculation remove spasm, increase the open number of capillary, accelerate VPV, make the red blood cell depolymerization of aggegation, play effect promoting blood circulation and removing blood stasis.
Trifoliate jewelvine is a kind of ancient Insecticiding-miticiding plant, its main active rotenone has been widely used in cultural control insect evil mite, existing rotenone is processed to cream preparation more and uses, because it has active high, the advantage such as nuisanceless, on economic crops and organic crops, application is more.
Yet there are no cnidium oil and rotenone composite or take that cnidium oil, rotenone are composite kills the aphid miticide as being mixed of basis.
Summary of the invention
The purpose of this invention is to provide and a kind ofly take botanical components cnidium oil and rotenone as killing the preparation method of aphid, acaricidal activity material and synergism compound preparation thereof.
In order to realize above-mentioned task, the present invention is achieved by following technical solution:
A kind of plant source synergistic compound aphicide miticide containing rotenone, it is characterized in that, it is main active that this plant source synergistic complex acaricide made be take cnidium oil and rotenone, the mass ratio of cnidium oil and rotenone is between 1:10～10:1, add additive compound to form, the preparation form is emulsifiable concentrate, microemulsion and aqueous emulsion; Wherein:
Described emulsifiable concentrate is prepared by following raw materials by weight:
Cnidium oil: 0.1%～50.0%, rotenone: 1.0%～10.0%, organic solvent: 0～30%, emulsifier: 5%～15%, synergist: 0～20%, bleeding agent: 5%～40%, the percentage by weight sum of raw material is 100%;
Described microemulsion is prepared by following raw materials by weight:
Cnidium oil: 0.1%～50.0%, rotenone: 1.0%～10.0%, organic solvent: 0～20%, surfactant: 5%～30%, synergist: 0～20%, antifreezing agent: 4%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Described aqueous emulsion is prepared by following raw materials by weight:
Cnidium oil: 0.1%～50%, rotenone: 1.0%～10.0%, organic solvent: 0～20%, surfactant: 5%～20%, synergist: 0～20%, antifreezing agent: 4%～10%, thickener: 0.2%～1%, surplus is water, the percentage by weight sum of raw material is 100%.
Above-mentioned organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, acetone, the mixture of one or more in dimethyl formamide;
Emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan series, tween;
The addition product that surfactant is alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and analog, castor oil and oxirane, any one or several mixture in aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Synergist is one or more the mixture in organosilicon, Butacide, Octacide 264, alkyl carbonate;
Antifreezing agent is ethylene glycol or propane diols;
Bleeding agent is one or more the mixture in azone, tung oil, epoxidized soybean oil or machine oil;
Thickener is one or more the mixture in polyvinyl alcohol, xanthans, sodium carboxymethylcellulose, Magnesiumaluminumsilicate.
The above-mentioned acaricidal preparation method of plant source synergistic compound aphicide containing rotenone is characterized in that the preparation form is emulsifiable concentrate, microemulsion and aqueous emulsion, wherein:
The preparation method of emulsifiable concentrate is: according to formula ratio, cnidium oil, rotenone, organic solvent, emulsifier, synergist, bleeding agent are mixed, stir in reactor, after quality inspection, finally make finished product.
The preparation method of microemulsion is: according to formula ratio, cnidium oil, rotenone, organic solvent, surfactant, synergist, antifreezing agent and water are mixed, stir transparently in reactor, after quality inspection, finally make finished product.
The preparation method of aqueous emulsion is: according to formula ratio by cnidium oil, rotenone, organic solvent, surfactant, synergist, antifreezing agent, thickener and and water mix, emulsion dispersion under the effect of high-speed shearing machine, after quality inspection, make finished product.
Plant source synergistic compound aphicide miticide of the present invention is a kind of novel aphid miticide that kills, and can prevent and treat the aphids such as the little Macrosiphus spp of apple yellow aphid, cabbage aphid, chrysanthemum on fruit tree, vegetables, flowers and economic crops and matrimony vine aphid; Can prevent and treat the evil mite harm such as tetranychus viennensis, Tetranychus cinnabarinus and Tetranychus urticae, especially to becoming mite to there is higher control efficiency simultaneously.
Embodiment
Take cnidium oil and rotenone as active component, the part by weight of cnidium oil and rotenone is 1:10～10:1 preparation, add a certain proportion of solvent and cosolvent and other insecticides adjuvant, carry out formulation, can make plant source synergistic complex acaricide, its formulation is environment-friendly type emulsifiable concentrate, microemulsion or aqueous emulsion.
1) plant source synergistic compound aphicide miticide environment-friendly type missible oil, cnidium oil content by weight 0.1～50%, rotenone 1.0～10.0%, all the other are auxiliary agents.Auxiliary agent comprises emulsifier, organic solvent, synergist, bleeding agent.
Emulsifier: 5～15%, be one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan series, tween;
Organic solvent: 0～30%, be one or more the mixture in ethyl acetate, acetone, ethanol;
Synergist: be one or more the mixture in Butacide, Octacide 264, alkyl carbonate;
Bleeding agent: be one or more the mixture in azone, tung oil, epoxidized soybean oil or machine oil.
2) plant source synergistic compound aphicide miticide microemulsion, cnidium oil content by weight 0.1～50%, rotenone 1.0～10.0%; All the other are auxiliary agents, and auxiliary agent comprises organic solvent, surfactant, synergist, antifreezing agent, water.
Surfactant: 5～30%, be the addition product of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane, any one or more in aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Organic solvent: 0～20%, be any one or several mixture in methyl alcohol, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO), ethyl acetate, acetone, ethanol;
Synergist: 0～20%, be one or more the mixture in Butacide, Octacide 264, alkyl carbonate;
Antifreezing agent: 4～10%, can be any in ethylene glycol, propane diols;
Water polishing 100%.
3) plant source synergistic compound aphicide miticide aqueous emulsion, cnidium oil content by weight 0.1～50%, rotenone 1.0～10.0%; All the other are auxiliary agents, and auxiliary agent comprises organic solvent, surfactant, synergist, antifreezing agent, thickener, water;
Surfactant: 5～20%, be the addition product of alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and analog, castor oil and oxirane, any one or several mixture in aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Organic solvent: 0～20%, be any one or several mixture in methyl alcohol, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO), ethyl acetate, acetone, ethanol;
Synergist: 0～20%, be one or more the mixture in Butacide, Octacide 264, alkyl carbonate;
Antifreezing agent: 4～10%, be any of ethylene glycol or propane diols;
Thickener: 0.2～1%, be any in xanthans, polyvinyl alcohol, sodium carboxymethylcellulose, Magnesiumaluminumsilicate;
Water polishing 100%.
In preparation process, can also add as required some other auxiliary agents, other auxiliary agent content is 0～5%, comprises synergist, wetting agent, density adjuster.
The production method of above-mentioned plant source synergistic compound aphicide miticide cream preparation is: each component, by formula weighing medicament, is mixed, and stirred in reactor, after quality inspection, finally make finished product.
The production method of above-mentioned plant source synergistic compound aphicide miticide microemulsion is: each component, by formula weighing medicament, is mixed, stir transparently in reactor, after quality inspection, finally make finished product.
The production method of above-mentioned plant source synergistic compound aphicide miticide aqueous emulsion is: each component, by formula weighing medicament, is mixed, and finally emulsion dispersion under the effect of high-speed shearing machine, after quality inspection, finally make finished product.
eXPERIMENTAL EXAMPLE
The inventor is through studying cnidium oil and rotenone, determine that both are after rationally composite, the environment-friendly type missible oil, microemulsion or the aqueous emulsion that by adding auxiliary agent, are processed into, for preventing and treating various agricultural insect, evil mite, especially have good control efficiency for phytophagous mites, aphid.
In order better to understand essence of an invention, below with example, describe the technology contents of invention in detail, but the content of invention is not limited to this.
embodiment 1: cnidium oil and rotenone are composite to the indoor co-toxicity research of Tetranychus cinnabarinus
1.1 materials and methods
For trying the evil mite: Tetranychus cinnabarinus (
tetranychus cinnabarinusboisduval), Nuisanceless Pesticide Research Service Center, Xibei Agroforestry Science and T's greenhouse cultivation.
Reagent agent:
A. cnidium oil, Guangxi permanent letter spices company provides, Ligustilide content > 35%.
B. rotenone, agriculture Co., Ltd of Guangxi Xerox provides, active constituent content >=98%.
1.2 assay method
Employing is soaked slide method the evil mite is carried out to indoor bioassay.The segment that two-sided tape is cut into to two centimeter length is attached to microslide one end, then tears the scraps of paper on viscose glue off.Choose healthy one-tenth mite, provoke with little writing brush, its back is attached on viscose glue, notice that mite foot, antenna are not stuck.Sticky 30 of every slide, apportion three row, first have or not dead mite with the anatomical lens inspection, and dead mite should be chosen and discards, and again mends and is stained with the healthy mite that becomes.Slide is soaked into the liquid, shakes gently, within 5 seconds, take out afterwards, with blotting paper suck the viscose glue surface and around liquid, calculation correction lethality after 4 hours.
1.3 co-toxicity evaluation
1.3.1 determining of two kinds of medicament rational proportions
With reference to the method for Mansour etc. and the method for Zhang Zong Ping, carry out.Be specially:
1. Mansour method: concrete grammar is: two kinds of single doses are made into respectively to finite concentration (examination worm lethality is between the concentration of 30-70%), and two kinds of single doses are mixed with to admixing medical solutions (concentration degree when in admixing medical solutions, each single dose concentration is alone with them is identical), measure respectively each single dose and the mixture indoor harmacological effect to the examination worm, biologicall test adopts 1 method.Whether judge synergy according to following formula:
Pa, Pb is respectively the examination worm lethality of each single dose;
Pass judgment on the synergy of two kinds of pharmacy mix with c.f value size, as c.f > be synergistic effect 20 the time, c.f<-20 o'clock are Hangzhoupro short of money effect ,-20<c.f<20 o'clock are summation action
2. Zhang Zong Ping method: first measure the virulence of each single dose to the evil mite, then take the two LC50 value is basis, measures the drug effect of both different proportionings combinations, and obtains prediction inhibiting rate and the toxicity ratio (surveying inhibiting rate/expection inhibiting rate) of each proportioning combination.When toxicity ratio>1, it is synergistic effect; Toxicity ratio<1 is antagonism; It when the toxicity ratio is 1 left and right, is summation action.Toxicity ratio soprano, be best proportioning.
1.3.2 the mensuration of mixture co-toxicity coefficient and calculating
Give birth to the survey method the same.Obtain the co-toxicity coefficient (CTC value) of various complex preparations by the SunShi method, and pass judgment on the synergistic effect of mixture with the CTC value.This patent is thought: as CTC > be synergistic effect 120 the time, CTC<80 o'clock are antagonism, 80<CTC<120 are summation action, CTC > remarkable synergistic effect 200 o'clock arranged.
1.4 results and analysis
1.4.1 cnidium oil and rotenone are to Tetranychus cinnabarinus Toxicity Determination result
Table 1 cnidium oil and all medicaments of rotenone two are to Tetranychus cinnabarinus indoor bioassay result
1.4.2 the measurement result of mixed collaborative toxicity index (cf) between cnidium oil and rotenone
Cnidium oil and two kinds of pharmacy mix of rotenone are carried out to indoor bioassay, obtain each mixed cf value, table 2 as a result.
Table 2: the cf value (Tetranychus cinnabarinus) be mixed between cnidium oil and rotenone
As can be seen from the above table, by the synergism test that is mixed between cnidium oil and two kinds of medicaments of rotenone, this combination has synergistic effect, and mixed cf value reaches 24.25.
For above result, next step will be studied the synergistic relationship between its concrete mixed ratio.
1.4.3 determining of mixture rational proportion
According to the above results, carried out definite test of rational proportion between cnidium oil and rotenone, concrete measurement result is as table 3.
Table 3 cnidium oil proportionings different from rotenone are to the Tetranychus cinnabarinus bioassay results
Annotate: cnidium oil medicament LC
50for 2308.95mg/L, rotenone medicament LC
50for 77.83mg/L, during practical measurement, cnidium oil medicament LC
50for 2300mg/L, rotenone medicament LC
50for 80mg/L.
As shown in Table 3, rotenone and cnidium oil LC
50all showing addition or synergistic effect in the time of between 9:1～1:9, is wherein 1:7.19～1:12.32 in 8:2～7:3(active ingredient proportioning) synergy is the most remarkable down.
1.4.4 the measurement result of cnidium oil and rotenone mixture co-toxicity coefficient (CTC)
According to above measurement result, configured 30% Ligusticum wallichii
.rotenone emulsifiable solution, 20% Ligusticum wallichii
.rotenone micro-emulsion, 20% Ligusticum wallichii
.rotenone water emulsion and 10% Ligusticum wallichii
.rotenone emulsifiable solution, measured respectively the indoor virulence of 4 kinds of complex preparations to Tetranychus cinnabarinus, and tried to achieve the CTC value with the SunShi method, and concrete outcome is in Table 4.
table 4: cnidium oil and rotenone and complex preparation thereof the Toxicity Determination result to Tetranychus cinnabarinus
As shown in Table 4, the co-toxicity coefficient of 4 built agents is respectively 276.75,256.91,243.55 and 300.47, all is greater than 200, shows as remarkable synergistic effect.
embodiment 2: cnidium oil and rotenone are composite to the indoor joint toxicity measuring of apple yellow aphid
When carrying out above-mentioned research, the applicant be take apple yellow aphid as the examination worm, adopt method and the program of " embodiment 1 ", system testing cnidium oil synergistic effect to aphid mixed with rotenone, drawn conclusion similar to Example 1, cnidium oil and rotenone are composite to the remarkable synergistic effect of apple yellow aphid tool.The present embodiment only provides the co-toxicity coefficient test result of 4 built agents to apple yellow aphid.
, test material
For trying aphid: apple yellow aphid (
aphis citricola van dergoot), pick up from Xibei Univ. of Agricultural & Forest Science & Technology gardening institute fruit tree experiment station.
Reagent agent:
Cnidium oil, Guangxi permanent letter spices company provides, Ligustilide content > 35%; Rotenone, agriculture Co., Ltd of Guangxi Xerox provides, active constituent content >=98%;
30% Ligusticum wallichii
.rotenone emulsifiable solution; 20% Ligusticum wallichii
.rotenone micro-emulsion; 20% Ligusticum wallichii
.rotenone water emulsion; 10% Ligusticum wallichii
.rotenone emulsifiable solution.By Xibei Univ. of Agricultural & Forest Science & Technology's public nuisance-free agricultural chemicals research center, provide.
, test method
soak the worm leaf dipping method:employing is soaked the worm leaf dipping method aphid is carried out to indoor bioassay.First reagent agent is diluted with water to mother liquor, then is diluted to 6-7 concentration gradient, the more related blade of examination worm of choosing is taken out and blots liquid with blotting paper after dipping 5s together in liquid, put into the 9cm culture dish that is covered with filter paper that moisturizing in advance is good.The first ware of every 30-40, every concentration is processed 90-120 aphid.Water treatment is used in contrast, checks examination worm death toll after 8h, if contrast lethality, higher than 20% test on the same day, cancel, otherwise after by the abott updating formula, lethality being proofreaied and correct, the calculation correction lethality.
the mensuration of mixture co-toxicity coefficient and calculating:give birth to the survey method the same.Obtain the co-toxicity coefficient (CTC value) of various complex preparations by the SunShi method, and pass judgment on the synergistic effect of mixture with the CTC value.This research is thought: as CTC > be synergistic effect 120 the time, CTC<80 o'clock are antagonism, 80<CTC<120 are summation action, CTC > remarkable synergistic effect 200 o'clock arranged.
, results and analysis
Employing is soaked the worm leaf dipping method and has been measured respectively the indoor virulence of 4 kinds of complex preparations to apple yellow aphid, and tries to achieve the CTC value with the SunShi method, the results are shown in Table 1.
table 1: cnidium oil and rotenone and complex preparation thereof the Toxicity Determination result to apple yellow aphid
As shown in Table 1, the co-toxicity coefficient of 4 built agents is respectively 318.36,327.58,289.20 and 295.05, all much larger than 200, shows as remarkable synergistic effect.
the preparation of embodiment 3:30% cnidium oil rotenone environment-friendly type missible oil
Under 20～25 ℃, get 30 parts of cnidium oils and rotenone, the composite ratio of cnidium oil and rotenone is 14:1, add successively 10 parts of azones on the said mixture basis, 20 parts of machine oil, 3 parts of neopelexes and 7 parts of OPEOs, ethyl acetate polishing 100%, stir 10～30 minutes with 800～1000 rev/mins of mixing speeds, can make 30% Ligusticum wallichii rotenone emulsifiable solution.
the preparation of embodiment 4:20% cnidium oil rotenone water emulsion
Under 20 ~ 25 ℃, get respectively 20 parts of cnidium oils and rotenone, the composite ratio of cnidium oil and rotenone is 9:1, add successively 5 parts of 2 parts of azones, styryl block polyethers in said mixture, 3 parts of polyoxyethylene nonylphenol ether phosphates, 5 parts of ethylene glycol, add after mixing containing 100 parts of 0.05% xanthan gum solution polishings, be to stir 20 minutes under 10000 rev/mins at the high-speed shearing machine rotating speed, can make 20% cnidium oil rotenone water emulsion.
the preparation of embodiment 5:20% Ligusticum wallichii rotenone micro-emulsion
Under 20-25 ℃, get respectively 18 parts of cnidium oils and 2 parts of rotenone, then add successively 5 parts of azones, 13 parts of calcium dodecyl benzene sulfonates, 2 parts of styrenated phenol polyoxyethylene (n30) ether, 2 parts of OPEOs in said mixture, 5 parts of ethylene glycol, mix 100 parts of rear water polishings, stir 10～30 minutes with 800～1000 rev/mins of mixing speeds, can make 20% cnidium oil rotenone micro-emulsion.
the preparation of embodiment 6:10% Ligusticum wallichii rotenone environment-friendly type missible oil
Under 20-25 ℃, get respectively 9 parts of cnidium oils and 1 part of rotenone, then add successively 10 parts of azones in said mixture, 25 parts of machine oil, 2 parts of neopelexes and 8 parts of OPEOs, ethyl acetate polishing 100%, stir 10～30 minutes with 800～1000 rev/mins of mixing speeds, can make 10% Ligusticum wallichii rotenone emulsifiable solution.
embodiment 7: the composite aphid miticide control Tetranychus cinnabarinus field control effectiveness test that kills of cnidium oil and rotenone
For the field efficacy situation of clear and definite plant source synergistic compound aphicide miticide to Tetranychus cinnabarinus, we have carried out the drug effect test of field zone of control of maize Tetranychus cinnabarinus in 2009 at Weinan City Dali County, Shaanxi Province Dong Niancun to this medicament.
, reagent agent
20% Ligusticum wallichii rotenone micro-emulsion, medicament code name A
20% Ligusticum wallichii rotenone water emulsion, medicament code name B
30% Ligusticum wallichii rotenone emulsifiable solution, medicament code name C
Avermectin 1.8% missible oil, Qianjiang, Zhejiang Province Biochemie S.A. produces.
, test article
3 of syringes (10ml), two of knapsack sprayers, 300 of drops, 1 of Enamel jar (1000ml), 3 of pencils, 1 of minute book, pulling needle is some, reaches one one, camera.
, for studying thing
Corn.
, experimental scheme and method
Small plot experiment is taked in this test, and 500,750,1000 times of reagent agents and 2000 times of 1.8% abamectin emulsifiable concentrates and 5 processing of blank are established in test altogether, and each processes 3 repetitions (being repetition of each corn behavior).The community area is 414.4m
2, evil mite number on any 10 strain corns of 1 row in the middle of the front investigation of spray medicine, after medicine, investigation in 1,3,7 day mite number alive, calculate preventive effect.
, spray pattern
Conventional spray.
, result of the test and statistics
As can be seen from Table 1, three of cnidium oil and rotenone complex preparations have shown good control efficiency to Tetranychus cinnabarinus.Medicament A, B, 500 times of liquid of C preparation after lower 3 days control efficiency all more than 80%, after 7 days, control efficiency reaches more than 90%, through significance of difference analysis, and contrast the medicament Avermectin without significant difference, the complex preparation that shows cnidium oil and rotenone combines has obvious field efficacy.By table 1, it can also be seen that, the quick-acting of this compound drug is obviously not as the contrast medicament, and this characteristic with botanical pesticide is relevant, but has certain lasting period, and through observing, the lasting period, about 10 days, is adapted at applying on economic crops and organic crops.
the field control effectiveness test result of aphid miticide to Tetranychus cinnabarinus killed in table 1:3 Plants source
Annotate: the mean value that 1. in the table, data are 3 repetitions; 2. in " preventive effect " hurdle, after ordered series of numbers, the identical lowercase person of mark is illustrated in variance analysis (DMRT method), on 5% level without significant difference.
embodiment 8: the composite aphid miticide control apple yellow aphid field control effectiveness test that kills of cnidium oil and rotenone
For the field efficacy situation of clear and definite plant source synergistic compound aphicide miticide to aphid, the applicant has carried out 3 Plants source synergistic complexes in 2010 at Shaanxi Province's Baishui County and has killed the drug effect test of field zone that the aphid miticide is prevented and treated apple yellow aphid.
, reagent agent
10% Ligusticum wallichii rotenone emulsifiable solution, medicament code name D
20% Ligusticum wallichii rotenone micro-emulsion, medicament code name A
30% Ligusticum wallichii rotenone emulsifiable solution, medicament code name C
20% Imidacloprid missible oil, Jiangsu Jiannong Agrochemical Co., Ltd..
, test article
Syringe (3 of 10ml), two of knapsack sprayers, drop (300), Enamel jar (1000ml1), 3 of pencils, 1 of minute book, pulling needle is some, reaches one one, camera.
, for studying thing
Apple tree
4, experimental scheme and method
Carry out the blade face conventional spray by 500,1000 and 1500 times of liquid measures of reagent agent, 20% 1000 times of Imidacloprid EC liquid is the contrast medicament, and establishes the clear water contrast, and every mu of fluid volume is 90Kg.Every 5-10 Wei Yige community, and establish the protection Hang， community be randomized arrangement, repeat 3 times.Strain mark 5-10 sheet is determined in every community the aphid blade, investigates full tree louse amount (every community borer population is no less than 200 as far as possible).Investigation insect population radix before medicine, investigation in 1,3,7 day borer population alive after medicine.Calculate the insect population decline rate and proofread and correct preventive effect, computing formula is as follows:
In formula, Ta: insect population number after chemicals treatment, Tb: insect population number before chemicals treatment, Ca: insect population number after control treatment, Cb: insect population number before control treatment.
, spray pattern
Conventional spray.
, test results and analysis
As can be seen from Table 1, three of cnidium oil and rotenone complex preparations have all shown good control efficiency to apple yellow aphid.Medicament A, C, 500-1000 times of liquid of D preparation after 3 days control efficiency all more than 90%, after 7 days, control efficiency reaches more than 95%, through significance of difference analysis, and contrast the medicament Imidacloprid without significant difference, the complex preparation that shows cnidium oil and rotenone combines has obvious field efficacy.By table 1, it can also be seen that, the quick-acting of this compound drug and lasting period are all better, and this may be relevant with the volatility of plants essential oil wherein used, is adapted at applying on economic crops and organic crops.
The field control effectiveness test result (2010 year 6-7 month Shaanxi plain boiled water) of aphid miticide to apple yellow aphid killed in the source synergy of table 1:3 Plants
Annotate: be the mean value of 3 repetition in table, mark identical minuscule person after preventive effect and be illustrated in variance analysis (DMRT method) on 5% level without significant difference.
Claims (3)
1. the plant source synergistic compound aphicide miticide containing rotenone, it is characterized in that, it is main active that this plant source synergistic complex acaricide made be take cnidium oil and rotenone, the mass ratio of rotenone and cnidium oil is 8:2 ~ 7:3, add additive compound to form, the preparation form is emulsifiable concentrate, microemulsion and aqueous emulsion; Wherein:
Described emulsifiable concentrate is prepared by following raw materials by weight:
Cnidium oil: 0.1%～50.0%, rotenone: 1.0%～10.0%, organic solvent: 0～30%, emulsifier: 5%～15%, synergist: 0～20%, bleeding agent: 5%～40%, the percentage by weight sum of raw material is 100%;
Described microemulsion is prepared by following raw materials by weight:
Cnidium oil: 0.1%～50.0%, rotenone: 1.0%～10.0%, organic solvent: 0～20%, surfactant: 5%～30%, synergist: 0～20%, antifreezing agent: 4%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Described aqueous emulsion is prepared by following raw materials by weight:
Cnidium oil: 0.1%～50%, rotenone: 1.0%～10.0%, organic solvent: 0～20%, surfactant: 5%～20%, synergist: 0～20%, antifreezing agent: 4%～10%, thickener: 0.2%～1%, surplus is water, the percentage by weight sum of raw material is 100%.
2. the plant source synergistic compound aphicide miticide containing rotenone as claimed in claim 1, is characterized in that, described organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, acetone, the mixture of one or more in dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween;
The addition product that described surfactant is alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane, any one or several mixture in aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Described synergist is one or more the mixture in organosilicon, Butacide, Octacide 264, alkyl carbonate;
Described antifreezing agent is ethylene glycol or propane diols;
Described bleeding agent is one or more the mixture in azone, tung oil, epoxidized soybean oil or machine oil;
Described thickener is one or more the mixture in polyvinyl alcohol, xanthans, sodium carboxymethylcellulose, Magnesiumaluminumsilicate.
3. the acaricidal preparation method of plant source synergistic compound aphicide containing rotenone claimed in claim 1, is characterized in that, the preparation form is emulsifiable concentrate, microemulsion and aqueous emulsion, wherein:
The preparation method of emulsifiable concentrate is: according to formula ratio, cnidium oil, rotenone, organic solvent, emulsifier, synergist, bleeding agent are mixed, stir in reactor, after quality inspection, finally make finished product;
The preparation method of microemulsion is: according to formula ratio, cnidium oil, rotenone, organic solvent, surfactant, synergist, antifreezing agent and water are mixed, stir transparently in reactor, after quality inspection, finally make finished product;
The preparation method of aqueous emulsion is: according to formula ratio by cnidium oil, rotenone, organic solvent, surfactant, synergist, antifreezing agent, thickener and and water mix, emulsion dispersion under the effect of high-speed shearing machine, after quality inspection, make finished product.
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| CN103518751A (en) * | 2013-10-28 | 2014-01-22 | 上海市农业科学院 | Plant composite insecticide |
| CN105165905A (en) * | 2015-10-08 | 2015-12-23 | 广西南宁益土生物科技有限责任公司 | Method for controlling aphid of green plants |
| CN108432817A (en) * | 2018-04-22 | 2018-08-24 | 陈太师 | A kind of acaricide and preparation method thereof |
| CN109042657A (en) * | 2018-07-04 | 2018-12-21 | 上海市农业科学院 | A kind of compound acaricide and its preparation method and application controlling phytophagous harmful mite |
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