CN104026132B - A kind of wheat cold-resistant conditioning agent preparation method - Google Patents
A kind of wheat cold-resistant conditioning agent preparation method Download PDFInfo
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- CN104026132B CN104026132B CN201410290668.4A CN201410290668A CN104026132B CN 104026132 B CN104026132 B CN 104026132B CN 201410290668 A CN201410290668 A CN 201410290668A CN 104026132 B CN104026132 B CN 104026132B
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Abstract
The invention belongs to plant growth regulator technical field, relate to a kind of wheat cold-resistant conditioning agent preparation method, first the sea grass polysaccharide prepared with hot water extraction method is carried out microwave degradation and prepare low-molecular-weight sea grass polysaccharide, again the low-molecular-weight sea grass polysaccharide of acquisition is dissolved in distilled water, the concentration making sea grass polysaccharide is 100 1000mg/L, is configured to sea grass polysaccharide solution and i.e. prepares cold-resistant conditioning agent；Or in sea grass polysaccharide solution, it is separately added into salicylic acid, brassinosteroid, glycine betaine and/or spermidine again, making Determination of Salicylic Acid is 0mg/L or 100 200mg/L, brassinosteroid concentration is 0mg/L or 0.01 0.1mg/L, glycine betaine concentration is 0mg/L or 100 1000mg/L, spermidine concentration is 0mg/L or 10 100mg/L, and the proportioning that finally stirs is made combination solution and i.e. prepared cold-resistant conditioning agent；Or adding surfactant again in combination solution, making surfactant concentration is 1000 20000mg/L, prepares cold-resistant conditioning agent after stirring；Its abundant raw material source, formulated component science, preparation technology is simple, and processing cost is low.
Description
Technical field:
The invention belongs to plant growth regulator technical field, relate to a kind of cold-resistant conditioning agent of wheat
Preparation method, component include sea grass polysaccharide or sea grass polysaccharide and salicylic acid, brassinosteroid,
The complex preparation of one or more in glycine betaine and/or spermidine, it is possible to be effectively improved wheat
Cold tolerance.
Background technology:
Enteromorpha is a kind of large-scale economic green alga, belongs to Chlorophyta Ulvales Ulvaceae Enteromorpha, algae
Body is grass green, and primary growth, in intertidal zone, has stronger fertility and environment resistance,
The whole year all can grow, and has a large amount of distribution at China coast；But China coastal seas bank marine site is even in recent years
The extensive green tide of sudden Enteromorpha of continuous outburst, causes pollution greatly to marine environment, at present
Common Enteromorpha Application way is to bury to make compost or dry to be ground into feed mostly, its technology content
Relatively low, utilization ratio is the highest, cause the serious wasting of resources, it is therefore desirable to exploitation makes new advances
Enteromorpha processes and application technology, to strengthen the high-valued exploitation application of Enteromorpha, alleviates offshore pollution.
The sea grass polysaccharide (EP) contained in Enteromorpha is one of Enteromorpha main active substances, and it has immunity
Regulation activity, the numerous feature such as cell-proliferation activity and antioxidation activity that promotees, and sea grass polysaccharide
As pure natural algal polysaccharides, at plant disease-resistant and degeneration-resistant aspect liquid, there is far-reaching application and dive
Power.Salicylic acid (SA) is induction plant disease-resistant and degeneration-resistant signal of interest molecule, it is possible to increase
Plant protection enzymatic activity, increases osmotic adjustment and its secondary metabolites content, thus improves
The disease resistance of plant and resistance；Brassinosteroid (BR) is that one has growth regulating to plant
The steroid compound of effect, it is possible to alleviate the environment stress injury effect to plant seedlings, its
As a kind of novel plant hormone, it is widely used improving to have on stress resistance of plant；Glycine betaine
(GB) it is a kind of quaternary ammonium type water-soluble alkaloid, is also a kind of atoxic osmotic field
Matter, it has obtained increasing attention in plant stress-resistance；Spermidine (Spd) is considered
It is the active material of similar second messenger, the resistance of induction plant is played an important role；On
State four kinds of materials the most nontoxic, harmless, pollution-free, it is possible to induction plant produces resistance, is green
The biogenic chemistry that colour circle is protected lures anti-material.
Wheat is the second largest cereal crops of China, and its breeding time is long, planted in fall, through Dong Lichun,
Summer gathers in the crops, and crosses over 2 years breeding time, and the four seasons containing the spring, summer, autumn and winter, at the early growth period of wheat
And the key developmental stages such as jointing of turning green all can experience the low temperature stress such as cold current, cold spell in later spring, Er Qieji
All winter wheat growing areas have damage to plants caused by sudden drop in temperature and freeze injury occur, leverage the product of Wheat Production
Matter and stable yield, serious threat grain security, current low temperature freezing-disaster become China's wheat
One of natural calamity maximum in production；Therefore, wheat low-temperature resistance freezing evil ability is strengthened to guarantor
Card improving yield of wheat stable yields, Ensuring Food Safety have important practical significance and using value；Real
Have in trampling Chinese patent 201310116703.6 disclose a kind of wheat low-temperature resistance conditioning agent and
Preparation method, concrete component include glycosaminoglycan 3～9g/L, Choline Chloride 20～
100g/L, salicylic acid or sodium salicylate 4～20g/L, potassium silicate 80～400g/L, table
Face activating agent 20～100g/L and water, but its complicated component, preparation method are loaded down with trivial details and effect
The best；Therefore the present invention seeks a kind of simple and easy to get, with low cost, green natural of design and resists
The preparation method of the cold-resistant conditioning agent of the significant wheat of cryogenic effect, tool is cultivated in the production for wheat
There is important economic implications.
Summary of the invention:
It is an object of the invention to the shortcoming overcoming prior art to exist, it is provided that a kind of wheat is cold-resistant
The preparation method of conditioning agent, it is possible to strengthen wheat seed or the multinomial physical signs of seedling, raising
Multiple activities of antioxidant enzymes, improves the ability of wheat opposing low temperature stress.
To achieve these goals, the one side of preparation of the cold-resistant conditioning agent of wheat that the present invention relates to
Method is: first the sea grass polysaccharide prepared with hot water extraction method carried out microwave degradation prepare molecular weight be
The low-molecular-weight sea grass polysaccharide of 1k-50kDa, then the low-molecular-weight sea grass polysaccharide of acquisition is dissolved in
In distilled water, the concentration making sea grass polysaccharide is 100-1000mg/L, is configured to sea grass polysaccharide molten
Liquid i.e. prepares cold-resistant conditioning agent.
The another kind of preparation method of the cold-resistant conditioning agent of wheat that the present invention relates to is: by low-molecular-weight
Sea grass polysaccharide is dissolved in distilled water that to be configured to the sea grass polysaccharide that concentration is 100-1000mg/L molten
Liquid, then be separately added in sea grass polysaccharide solution salicylic acid, brassinosteroid, glycine betaine and/
Or spermidine, making Determination of Salicylic Acid is 0mg/L or 100-200mg/L, and brassinosteroid is dense
Degree is 0mg/L or 0.01-0.1mg/L, and glycine betaine concentration is 0mg/L or 100-1000mg/L,
Spermidine concentration is 0mg/L or 10-100mg/L, and the proportioning that finally stirs is made compounding molten
Liquid i.e. prepares cold-resistant conditioning agent.
The another kind of preparation method of the cold-resistant conditioning agent of wheat that the present invention relates to is: by low-molecular-weight
Sea grass polysaccharide is dissolved in distilled water that to be configured to the sea grass polysaccharide that concentration is 100-1000mg/L molten
Liquid, then be separately added in sea grass polysaccharide solution salicylic acid, brassinosteroid, glycine betaine and/
Or spermidine, making Determination of Salicylic Acid is 0mg/L or 100-200mg/L, and brassinosteroid is dense
Degree is 0mg/L or 0.01-0.1mg/L, and glycine betaine concentration is 0mg/L or 100-1000mg/L,
Spermidine concentration is 0mg/L or 10-100mg/L, and the proportioning that finally stirs is made compounding molten
Liquid, then in combination solution, add surfactant, make the surfactant concentration be
1000-20000mg/L, prepares cold-resistant conditioning agent after stirring.
The surfactant that the present invention relates to be TWEEN Series, Span series, OP series and/or
The mixture of one or more in NP series；The user of the cold-resistant conditioning agent of wheat prepared
Method is for spray wheat seedling/to irrigate, or carries out wheat seed soaking seed after 6-12h
Sowing.
The present invention is compared with prior art and product, by sea grass polysaccharide and salicylic acid, rape element
Lactone, glycine betaine and spermidine etc. are biogenic lures anti-material to compound, and makes cold-resistant conditioning agent,
The plant comprehensive resistance to low temperature stress can be improved, it is ensured that plant normal growth is grown；Institute
Select raw material Enteromorpha abundance, with low cost, environmental friendliness；Prepare the method letter of conditioning agent
Single easy, it is simple to popularization and application.
Detailed description of the invention:
Below by embodiment, the present invention is described in further detail, but the present invention does not limit
In following embodiment.
Embodiment 1:
The sea grass polysaccharide prepared with hot water extraction method is first carried out microwave degradation system by the present embodiment
Obtain the low-molecular-weight sea grass polysaccharide that molecular weight is 1k-50kDa, then the low-molecular-weight waterside that will obtain
Tongue polysaccharide is dissolved in distilled water, and making sea grass polysaccharide concentration is 100-1000mg/L, i.e. prepares anti-
Cold conditioning agent A；Or be separately added in cold-resistant conditioning agent A again salicylic acid, brassinosteroid,
Glycine betaine and/spermidine any of which or multiple, concrete concentration is salicylic acid 0mg/L
Or 100-200mg/L, brassinosteroid 0mg/L or 0.01-0.1mg/L, glycine betaine 0mg/L
Or 100-1000mg/L, spermidine 0mg/L or 10-100mg/L, i.e. prepare cold-resistant regulation
Agent B；Or in cold-resistant conditioning agent A or cold-resistant conditioning agent B, it is separately added into surfactant again,
One in TWEEN Series, Span series, OP series and/or NP series selected by surfactant
Kind or multiple mixture, making surfactant concentration is 1-20g/L, i.e. prepares cold-resistant regulation
Agent C；Above-mentioned cold-resistant conditioning agent A, cold-resistant conditioning agent B and cold-resistant conditioning agent C all can effectively increase
Strong wheat seed or every physical signs of seedling, and improve multiple activities of antioxidant enzymes, to carry
Rise the cold tolerance of wheat, promote that it grows.
Embodiment 2:
The present embodiment first weighs the sea grass polysaccharide that 100mg molecular weight is 5kDa and is dissolved in 800ml
In distilled water, add 1000mg Tween-20 and stir, finally using distilled water constant volume
To 1L, after stirring, prepare the cold-resistant conditioning agent of wheat.
Embodiment 3:
The present embodiment first weighs the sea grass polysaccharide that 100mg molecular weight is 5kDa and is dissolved in 800ml
Distilled water prepares sea grass polysaccharide solution, then weighing 100mg salicylic acid, to be dissolved in above-mentioned Enteromorpha many
In sugar juice, it is subsequently adding 1000mg Tween-20 and stirs, finally fixed with distilled water
Hold to 1L, after stirring, prepare the cold-resistant conditioning agent of wheat.
Embodiment 4:
The present embodiment first weighs the sea grass polysaccharide that 100mg molecular weight is 5kDa and is dissolved in 800ml
Distilled water prepares sea grass polysaccharide solution, then weighs 0.01mg brassinosteroid and be dissolved in above-mentioned waterside
In tongue polysaccharide solution, it is subsequently adding 5000mg Arlacel-60 and stirs, finally with distillation
Water is settled to 1L, prepares the cold-resistant conditioning agent of wheat after stirring.
Embodiment 5:
The present embodiment first weighs the sea grass polysaccharide that 100mg molecular weight is 50kDa and is dissolved in 800ml
Distilled water prepares sea grass polysaccharide solution, then weighs 10mg spermidine and be dissolved in above-mentioned sea grass polysaccharide
In solution, it is subsequently adding 5000mg OP-7 and stirs, being finally settled to distilled water
1L, prepares the cold-resistant conditioning agent of wheat after stirring.
Embodiment 6:
The present embodiment first weighs the sea grass polysaccharide that 1000mg molecular weight is 5kDa and is dissolved in 800ml
Distilled water prepares sea grass polysaccharide solution, then weighing 100mg glycine betaine, to be dissolved in above-mentioned Enteromorpha many
In sugar juice, it is subsequently adding 5000mg NP-7 and stirs, being finally settled to distilled water
1L, prepares the cold-resistant conditioning agent of wheat after stirring.
Embodiment 7:
The present embodiment to be in wheat seedling embodiment 2-6 of Seedling Stage prepare cold-resistant
Conditioning agent carries out sprinkling process, and specific experiment is as follows::
(1) experimental technique: by wheat seed sterilization, immersion, vernalization, treat that seed just shows money or valuables one carries unintentionally,
Select wheat seed full, uniform and sow in culture dish, 20, every ware, use
Hoagland (Gram suddenly) nutrient solution is cultivated in illumination box, and condition of culture is
25/20 DEG C (day night), intensity of illumination 300 μm ol/m2/s, periodicity of illumination 14/l0h (day night),
Relative humidity 65 ± 5%；When wheat seedling growth to 21 heart stage of leaf, use embodiment 2-6
In prepare cold-resistant conditioning agent wheat seedlings blade is sprayed, make blade the most moistening, but
Liquid does not flows down, and negative control group spray is with the clear water of equivalent；After 24h, wheat seedling is placed in 4 DEG C
Low temperature stress 3d, then measures wheat seedlings blade indices；
(2) experimental result: 1. MDA (MDA) is the product of Lipid peroxidation metabolism under salt stress
Thing, the big I of its content represents the size of membrane damage degree；In this experiment under low temperature stress,
MDA content significantly raises, and can substantially alleviate low temperature stress pair after cold-resistant conditioning agent processes
The injury of cell membrane；Under the conditions of low temperature stress, wheat seedlings blade soluble sugar and solubility egg
Bai Hanliang substantially reduces, and can improve the two content after cold-resistant conditioning agent processes, and improves plant
Body overall metabolic level, and maintain plant osmosis regulating power；Cold-resistant conditioning agent can also improve
Wheat seedlings blade chlorophyll content, to promote photosynthesis, it is ensured that plant normal growth is grown；
Concrete comparing result is as shown in table 1:
The impact on wheat seedlings blade indices of the table 1 wheat cold-resistant conditioning agent
2. superoxide dismutase (SOD), peroxidase (POD) and peroxidase (CAT)
Collectively constitute the enzymatic protective system of plant, it is possible to catalysis active oxygen generation disproportionated reaction generates nontoxic
Molecular oxygen and water；In this test under the stimulation of low temperature stress, the activity of SOD and CAT has
Improved, to protect plant cell membrane, through cold-resistant conditioning agent process after, SOD, CAT and
POD activity is notable to be raised, and to protect cell membrane not damaged by free radical, improves plant pair
The resistivity of low temperature stress；Concrete comparing result is as shown in table 2:
The impact on wheat seedlings blade antioxidase of the table 2 wheat cold-resistant conditioning agent
3. this experiment is after low temperature stress 3d, and wheat seedling moves to cultivate under normal temperature condition 7d,
Measure its height of seedling, weight in wet base again；Then with after 105 DEG C of oven for drying 2h to constant weight, then measure
Dry weight；Result shows that the height of seedling of wheat seedling, seedling weight average have bright after cold-resistant conditioning agent processes
Aobvious increase, illustrates that sea grass polysaccharide can improve the ability of wheat seedling opposing low temperature stress, to protect
Demonstrate,prove its normal growth；Concrete comparing result is as shown in table 3:
The impact on wheat seedling biomass of the table 3 wheat cold-resistant conditioning agent
Claims (2)
1. a wheat cold-resistant conditioning agent preparation method, it is characterised in that concretely comprise the following steps:
First the sea grass polysaccharide prepared with hot water extraction method carried out microwave degradation prepare molecular weight be
The low-molecular-weight sea grass polysaccharide of 1k-50kDa, then the low-molecular-weight sea grass polysaccharide of acquisition is dissolved in
In distilled water, the concentration making sea grass polysaccharide is 100-1000mg/L, is configured to sea grass polysaccharide molten
Liquid；Salicylic acid, brassinosteroid, glycine betaine and Asia it is separately added into again in sea grass polysaccharide solution
Spermine, making Determination of Salicylic Acid is 100-200mg/L, and brassinosteroid concentration is
0.01-0.1mg/L, glycine betaine concentration is 100-1000mg/L, and spermidine concentration is
10-100mg/L, finally stirs and is configured to combination solution；Table is added again in combination solution
Face activating agent, making surfactant concentration is 1000-20000mg/L, prepares after stirring
Cold-resistant conditioning agent.
Wheat the most according to claim 1 cold-resistant conditioning agent preparation method, its feature exists
In described surfactant is TWEEN Series, Span series, OP series and/or NP series
One or more mixture.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104396547B (en) * | 2014-12-11 | 2017-01-11 | 朱小丽 | Method for improving chilling resistance of plant via glycine betaine |
| CN107183050A (en) * | 2017-05-27 | 2017-09-22 | 兰溪市酉泽饲料技术服务有限公司 | Wheat low-temperature resistance conditioning agent and its application method |
| CN111011394B (en) * | 2019-12-12 | 2021-02-02 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
| CN114304157B (en) * | 2020-10-10 | 2023-02-07 | 中国农业大学 | High-temperature-resistant grouting-promoting plant growth regulator and application thereof to crops |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102487943A (en) * | 2011-12-14 | 2012-06-13 | 中国科学院遗传与发育生物学研究所 | Composition capable of increasing wheat yield, method of preparing composition and application of composition |
| CN102898536A (en) * | 2012-10-19 | 2013-01-30 | 中国科学院海洋研究所 | Method for degrading enteromorpha polysaccharide |
| CN103202315A (en) * | 2013-04-07 | 2013-07-17 | 江苏徐淮地区淮阴农业科学研究所 | Low-temperature-resistant modifier of wheat and preparation method of modifier |
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