CN103461088B - Method for improving agar content of gracilaria lemaneiformis - Google Patents
Method for improving agar content of gracilaria lemaneiformis Download PDFInfo
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- CN103461088B CN103461088B CN201310400083.9A CN201310400083A CN103461088B CN 103461088 B CN103461088 B CN 103461088B CN 201310400083 A CN201310400083 A CN 201310400083A CN 103461088 B CN103461088 B CN 103461088B
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- asparagus
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Abstract
The invention provides a method for improving agar content of gracilaria lemaneiformis. The method includes: placing the gracilaria lemaneiformis cultivated in a sea area into plant hormone culture solution at night for first cultivation prior to temporary cultivation in the sea area, placing the gracilaria lemaneiformis into the plant hormone culture solution at the same night for second cultivation prior to placing back in the sea area for temporary cultivation, and then at an interval of two days, placing the gracilaria lemaneiformis into the plant hormone culture solution for third cultivation and the fourth cultivation prior to placing back to the sea area for cultivation. The plant hormone culture solution is 2,4-epibrassinolide solution 0.1-1.0mg/L in concentration, and the 2,4-epibrassinolide solution is prepared by firstly dissolving 2,4-epibrassinolide into ethyl alcohol 70-80% in volume concentration to prepare mother solution 1000mg/L in 2,4-epibrassinolide concentration and finally adding clean sea water. After nine days of cultivation of the plant hormone culture solution for four times, the agar content of the gracilaria lemaneiformis can reach a maximum of 21.65%.
Description
Technical field
The present invention relates to the breeding method of asparagus, be specifically related to a kind of method improving asparagus agar content.
Background technology
Asparagus (
gracilaria lemaneiformis) be a kind of important large-scale economical alga, be mainly used in extracting agar-agar and the bait as famous and precious marine products such as abalones.Asparagus originates in the Shandong Peninsula, and the water temperature of asparagus optimum growth, at 18-26 DEG C, grows and is restricted, affect the breeding scale of asparagus under too high or too low temperature condition.The seed selection success of high temperature resistant asparagus; if publication number is the application for a patent for invention of CN101564004; disclose the mutagenesis of asparagus through MNNG; cultivate at the high temperature of HYP again; obtain resistant to elevated temperatures asparagus, so also carry out large-scale cultivation asparagus at the Guangdong of south China, Fujian and Zhejiang Sea Area at present.But the agar content of asparagus is still lower, agar content is generally about 18% (dry weight), and simultaneously asparagus is high temperature resistant and cryogenic effect is also poor, and can only aestivate in the north, south passes the winter, overall culturing time is shorter.
Brassinosteroid is a kind of containing polyhydric phytosterin compound, and molecular formula is C
28h
48o
6, be a class novel plant hormone.In the 20 kinds of artificial rape chlorins compounds synthesized at present, best with 2,4-epi-brassinolide effect.In higher plant, 24-epi-brassinolide can improve plant physiology metabolic function, the process that coordinate plant growth is grown, promote plant cell elongation growth, promote cell division, the differentiation of organic matter Cumulate Sum tissue etc., thus improve the quality and yield of plant, also can improve plant to the resistance under the adverse circumstances such as high temperature, high salt, heavy metal.If publication number is the application for a patent for invention of CN102523852, disclosing by executing 2,4-epi-brassinolide outside root under high temperature stress, improving the warm tolerance of muskmelon.
Summary of the invention
The object of this invention is to provide a kind of method improving asparagus agar content, after the method process, cultivate the 9th day, asparagus agar content can reach 19.47% ~ 21.65%(dry weight).
Technical problem to be solved by this invention is a kind of method improving asparagus agar content, the asparagus that sea area cultivates is put into plant hormone culture fluid and cultivates 8 ~ 12 hours first by evening, then by this asparagus at holding culture offshore, this asparagus is put into plant hormone culture fluid second incubation 8 ~ 12 hours by that night again, put back to holding culture offshore, then with the interval time of 2 days, at night this asparagus is put into plant hormone culture fluid to carry out cultivating 8 ~ 12 hours for three times and cultivating 8 ~ 12 hours for four times, put back to sea area cultivation, described plant hormone culture fluid is concentration is 2 of 0.1 ~ 1.0 mg/L, 4-epi-brassinolide solution, these are 2 years old, 4-epi-brassinolide solution is 2, it is 70 ~ 80% alcohol that 4-epi-brassinolide is first dissolved in concentration expressed in percentage by volume, be made into 2, 4-epi-brassinolide concentration is the mother liquor of 1000 mg/L, add clean seawater more formulated.
Compared with prior art the invention has the advantages that a kind of method improving asparagus agar content, the asparagus that sea area cultivates is put into plant hormone culture fluid and cultivates 8 ~ 12 hours first by evening, then by this asparagus at holding culture offshore, this asparagus is put into plant hormone culture fluid second incubation 8 ~ 12 hours by that night more again, put back to holding culture offshore, then with the interval time of 2 days, at night this asparagus is put into plant hormone culture fluid to carry out cultivating 8 ~ 12 hours for three times and cultivating 8 ~ 12 hours for four times, put back to sea area cultivation, plant hormone culture fluid is concentration is 2 of 0.1 ~ 1.0 mg/L, 4-epi-brassinolide solution, these are 2 years old, 4-epi-brassinolide solution is 2, it is in 70 ~ 80% alcohol that 4-epi-brassinolide is first dissolved in concentration expressed in percentage by volume, be made into 2, 4-epi-brassinolide concentration is the mother liquor of 1000 mg/L, add clean seawater more formulated, 9 days are cultivated like this after the process of four plant hormone culture fluids, asparagus agar content is the highest can reach 21.65%(dry weight), growth rate also improves simultaneously.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
The maximum air temperature is 30 ~ 35 DEG C of sea areas by day, by the asparagus of high temperature injury, time at night, the asparagus that this sea area cultivates is put into plant hormone culture fluid and cultivates 8 ~ 12 hours first, then by this asparagus at holding culture offshore, then again this asparagus is put into plant hormone culture fluid second incubation 8 ~ 12 hours again evening, put back to sea area cultivation, then 2 days, interval, at night this asparagus is put into plant hormone culture fluid and carry out three cultivations 8 ~ 12 hours, put back to sea area cultivation, 2 days, interval again, at night this asparagus is put into plant hormone culture fluid and carry out four cultivations 8 ~ 12 hours, put back to sea area cultivation, in plant hormone culture fluid 2, 4-epi-brassinolide concentration is 0.1mg/L or 0.5mg/L or 1.0mg/L, this plant hormone culture fluid is 2, it is 70 ~ 80% alcohol that 4-epi-brassinolide is first dissolved in concentration expressed in percentage by volume, be made into 2, 4-epi-brassinolide concentration is the mother liquor of 1000 mg/L, add clean seawater more formulated.Control group is the asparagus that same sea area normally cultivates.After 4 process of plant hormone culture fluid, cultivation the 9th day, obtains the result as table 1;
Table 1: on the asparagus agar content of high temperature stress, the impact of growth rate in embodiment 1
| ? | Control group | 0.1 mg/L process | 0.5 mg/L process | 1.0 mg/L process |
| Relative growth rate | 2.51±0.28 | 3.43±0.18 | 3.92±0.41 | 3.62±0.17 |
| Agar content (%) | 17.67±1.10 | 20.06±1.33 | 21.53±0.81 | 19.80±1.60 |
As can be seen from Table 1 after four process of plant hormone culture fluid, the highest 21.53%(2 that can reach asparagus dry weight of processed group asparagus agar content, during 4-epi-brassinolide concentration 0.5 mg/L), control group is 17.67%, and heat-resisting ability strengthens during the cultivation of this asparagus, the high temperature of less than 35 DEG C can be tolerated, effectively alleviate the high temperature stress effect of asparagus.Its relative growth rate can reach 3.92, and control group only 2.51.
Embodiment 2
Substantially identical with embodiment, difference be daytime the minimum air temperature be 15 DEG C of sea areas, by the asparagus cultivation after plant hormone culture fluid four times process the 9th day of injury from low temperature, obtain the result as table 2;
Table 2: on the asparagus agar content of low temperature stress, the impact of growth rate in embodiment 2
| ? | Control group | 0.1 mg/L process | 0.5 mg/L process | 1.0 mg/L process |
| Relative growth rate | 3.12±0.24 | 3.78±0.40 | 4.36±0.20 | 3.61±0.12 |
| Agar content (%) | 18.07±0.21 | 20.87±0.50 | 21.65±0.69 | 19.47±0.53 |
As can be seen from Table 2 after four process of plant hormone culture fluid, the processed group asparagus content the highest 21.65%(2 that can reach asparagus dry weight, 4-epi-brassinolide concentration 0.5 mg/L), control group is 18.07%.And during the cultivation of this asparagus, low temperature tolerance ability strengthens, and can tolerate the low temperature of 15 DEG C, effectively alleviates the low temperature stress effect of asparagus, can extend asparagus culturing time.Its relative growth rate is 4.36, and control group is only 3.12.
Embodiment 3
Substantially identical with embodiment, difference be daytime the maximum air temperature be 25 DEG C of sea areas, the cultivation after plant hormone culture fluid four times process the 9th day of unharmed asparagus, obtains the result as table 3;
Table 3: on the asparagus agar content of normal temperature, the impact of growth rate in embodiment 3
| ? | Control group | 0.1 mg/L process | 0.5 mg/L process | 1.0 mg/L process |
| Relative growth rate | 5.78±0.20 | 6.16±0.27 | 6.48±0.43 | 5.95±0.38 |
| Agar content (%) | 18.27±0.12 | 20.6±0.53 | 20.93±0.42 | 19.67±0.32 |
As can be seen from Table 3 after four process of plant hormone culture fluid, the processed group asparagus agar content the highest 20.93%(2 that can reach asparagus dry weight, 4-epi-brassinolide concentration 0.5 mg/L), control group is 18.27%.Its relative growth rate is 6.48, and control group is only 5.78.
Claims (2)
1. one kind is improved the method for asparagus agar content, it is characterized in that the asparagus that sea area cultivates being put into plant hormone culture fluid and cultivating 8 ~ 12 hours first evening, then by this asparagus at holding culture offshore, this asparagus is put into plant hormone culture fluid second incubation 8 ~ 12 hours by that night again, put back to holding culture offshore, then 2 days, interval, at night this asparagus is put into plant hormone culture fluid and carry out three cultivations 8 ~ 12 hours, put back to holding culture offshore, 2 days, interval again, at night this asparagus is put into plant hormone culture fluid and carry out four cultivations 8 ~ 12 hours, put back to sea area cultivation, described plant hormone culture fluid is concentration is 2 of 0.1 ~ 1.0 mg/L, 4-epi-brassinolide solution, these are 2 years old, 4-epi-brassinolide solution is 2, it is 70 ~ 80% alcohol that 4-epi-brassinolide is first dissolved in concentration expressed in percentage by volume, be made into 2, 4-epi-brassinolide concentration is the mother liquor of 1000 mg/L, add clean seawater more formulated.
2. a kind of method improving asparagus agar content as claimed in claim 1, is characterized in that described plant hormone culture fluid to be concentration is 2, the 4-epi-brassinolide solution of 0.4 ~ 0.6 mg/L.
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| CN104336549A (en) * | 2014-10-10 | 2015-02-11 | 嵊泗县冠岛水产有限公司 | Processing method of asparagus |
| CN105210843A (en) * | 2015-10-12 | 2016-01-06 | 莆田市正洋水产发展有限公司 | The cultural method of a kind of asparagus |
| CN105794626A (en) * | 2016-03-23 | 2016-07-27 | 宁波大学 | Method for promoting diffusion of altar laver shell protonema conchospore |
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| CN101715723A (en) * | 2009-12-24 | 2010-06-02 | 广州大学 | Method for artificially culturing asparagus by controlling water flow |
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