CN107242250A - A kind of wheat cold-resistant derivant and its application process - Google Patents
A kind of wheat cold-resistant derivant and its application process Download PDFInfo
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- CN107242250A CN107242250A CN201710461329.1A CN201710461329A CN107242250A CN 107242250 A CN107242250 A CN 107242250A CN 201710461329 A CN201710461329 A CN 201710461329A CN 107242250 A CN107242250 A CN 107242250A
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- resistant derivant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Abstract
The invention discloses a kind of wheat cold-resistant derivant, its raw material is made up of the component of following parts by weight：10 15 parts of amino-oligosaccharide, 5 10 parts of polyethylene glycol, 35 parts of lipase, 35 parts of ethyl ethane sulphonate, 35 parts of alginic acid, 0.5 1 parts of maleic hydrazide, 12 parts of emulsifying agent, 0.1 0.2 parts of thickener, 10 15 parts of water.In addition, the invention also discloses a kind of application process of wheat cold-resistant derivant.Originally wheat cold-resistant derivant low-temperature resistance effect of the invention is good, can strengthen the winter resistance of wheat, improves wheat low-temperature resistance stress ability, and harm of the reduction low temperature to wheat growth reduces low temperature and lost, improves wheat yield；And the wheat cold-resistant derivant raw material of the present invention is easy to get, preparation method is simple, environmentally safe using rear noresidue, with important economic benefit and environmental benefit.
Description
Technical field
The invention belongs to wheat cultivation technical field, and in particular to a kind of wheat cold-resistant derivant and its application process.
Background technology
Wheat is the second largest cereal crops of China, and its breeding time is long, planted in fall, through Dong Lichun, summer harvest, breeding time
Across 2 years, the four seasons containing spring, summer, autumn and winter, the key developmental stages such as the early growth period and jointing of turning green of wheat can all undergo cold current, fall
The influence of the low temperature stress such as cold spell in spring, late frost, and the growing area of nearly all winter wheat has and damage to plants caused by sudden drop in temperature and freeze injury, significantly shadow
The quality and yield for having rung Wheat Production is stable, has seriously threatened grain security, and current low temperature freezing-disaster has turned into China's wheat life
One of maximum natural calamity in production.In recent decades, China's wheat frost evil probability of happening be in obvious ascendant trend, into
One of major obstacle factor for some area restriction Wheat Productions, therefore the low temperature freezing-disasters such as frost damage harm skill is defendd in research
Art, raising cold tolerance, to ensureing that improving yield of wheat stable yields is significant.
At present it is published be applied to Wheat Production in cold-resistant derivant complicated component, preparation method is cumbersome, be produced into
This height and effect is not good, therefore, seeks a kind of simple and easy to get, with low cost, green natural and the significant wheat of low-temperature resistance effect
Cold-resistant derivant, the production for wheat is cultivated with important economic implications.
The content of the invention
The invention provides a kind of wheat cold-resistant derivant, the cold-resistant being applied in the prior art in Wheat Production is solved
Derivant complicated component, preparation method are cumbersome, production cost is high and the problem of not good effect.
First purpose of the present invention is to provide a kind of wheat cold-resistant derivant, its raw material by following parts by weight component
Composition：
10-15 parts of amino-oligosaccharide, 5-10 parts of polyethylene glycol, 3-5 parts of lipase, 3-5 parts of ethyl ethane sulphonate, alginic acid
3-5 parts, 0.5-1 parts of maleic hydrazide, 1-2 parts of emulsifying agent, 0.1-0.2 parts of thickener, 10-15 parts of water.
It is preferred that, the raw material of the wheat cold-resistant derivant is made up of the component of following parts by weight：Amino-oligosaccharide 12
Part, 8 parts of polyethylene glycol, 4 parts of lipase, 4 parts of ethyl ethane sulphonate, 4 parts of alginic acid, 0.5 part of maleic hydrazide, 1 part of emulsifying agent, thickening
0.15 part of agent, 12 parts of water.
It is preferred that, the lipase activity is 10000U/g.
It is preferred that, the surfactant is the one or more in Tween-40, Tween-60, Arlacel-60, Arlacel-80.
It is preferred that, the thickener is sodium alginate, casein, guar gum, chitosan, gum arabic, xanthans, big
One or more in soybean glue.
Second object of the present invention is to provide a kind of application process of wheat cold-resistant derivant, specifically according to following steps
Implement：
Step 1, every mu weighs 30-50g wheat cold-resistant derivants, is diluted with water standby after 500-1000 times；
Step 2, in 3-5 days before low temperature arriving, sprayed with the wheat cold-resistant derivant after dilution in step 1 small
Wheat stem and wheat leaf blade.
It is preferred that, the low temperature refers to environment temperature≤0 DEG C.
The wheat cold-resistant derivant of the present invention can be prepared using following methods：
Step 1, weigh the amino-oligosaccharide of respective amount, it is polyethylene glycol, lipase, ethyl ethane sulphonate, alginic acid, blue or green fresh
Element, emulsifying agent, thickener, water；
Step 2, the amino-oligosaccharide weighed in step 1, lipase is added in the water weighed in step 1, stirs, make
It is completely dissolved, and obtains the aqueous solution；
Step 3, the ethyl ethane sulphonate weighed in step 1, alginic acid, maleic hydrazide are added into the poly- second two that step 1 is weighed
In alcohol, stirring is completely dissolved it, obtains alcoholic solution；
Step 4, the aqueous solution of step 2 and the alcoholic solution of step 3 are mixed, is charged with emulsifying agent and thickening
Agent, stirs, that is, obtains the wheat cold-resistant derivant.
Compared with prior art, the beneficial effects of the present invention are：
1) the wheat cold-resistant derivant low-temperature resistance effect of the present invention originally is good, can significantly increase the winter resistance of wheat, improves small
Wheat low-temperature resistance coerces ability, and harm of the reduction low temperature to wheat growth reduces low temperature loss, improves wheat yield.
2) wheat cold-resistant derivant raw material of the invention is easy to get, and preparation method is simple, and applies convenient, can be large-scale
Produce and popularization and application, with important economic benefit.
3) wheat cold-resistant derivant safety and environmental protection of the invention, noresidue, and to wheat growth without poisoning, to people and animals' ratio
It is safer, it is environmentally safe, with important environmental benefit.
Embodiment
In order that those skilled in the art more fully understand that technical scheme can be practiced, with reference to specific
The invention will be further described for embodiment, but illustrated embodiment is not as a limitation of the invention.
Experimental method described in various embodiments of the present invention, is conventional method, agents useful for same is such as without spy unless otherwise specified
Different explanation, is conventional reagent.
Embodiment 1
A kind of wheat cold-resistant derivant, its raw material is made up of the component of following parts by weight：10 parts of amino-oligosaccharides, 10 parts
Polyethylene glycol, 5 parts of enzyme activities are 10000U/g lipase, 3 parts of ethyl ethane sulphonates, 5 parts of alginic acids, 0.5 part of maleic hydrazide, 1
Part Tween-40,0.2 part of sodium alginate, 15 parts of water.
The preparation method of wheat cold-resistant derivant is as follows：
Step 1, weigh 10 parts of amino-oligosaccharides, 10 parts of polyethylene glycol, 5 parts of enzyme activities for 10000U/g lipase, 3 parts
Ethyl ethane sulphonate, 5 parts of alginic acids, 0.5 part of maleic hydrazide, 1 part of Tween-40,0.2 part of sodium alginate, 15 parts of water；
Step 2, the amino-oligosaccharide weighed in step 1, lipase is added in the water weighed in step 1, stirs, make
It is completely dissolved, and obtains the aqueous solution；
Step 3, the ethyl ethane sulphonate weighed in step 1, alginic acid, maleic hydrazide are added into the poly- second two that step 1 is weighed
In alcohol, stirring is completely dissolved it, obtains alcoholic solution；
Step 4, the aqueous solution of step 2 and the alcoholic solution of step 3 are mixed, is charged with Tween-40 and sea
Mosanom, stirs, that is, obtains wheat cold-resistant derivant.
The application process of wheat cold-resistant derivant is as follows：
Step 1, every mu weighs 30g wheat cold-resistant derivants, is diluted with water standby after 500 times；
Step 2, in 3 days before low temperature arriving, wheat is sprayed with the wheat cold-resistant derivant after being diluted in step 1
Stem and wheat leaf blade.
Embodiment 2
A kind of wheat cold-resistant derivant, its raw material is made up of the component of following parts by weight：12 parts of amino-oligosaccharides, 8 parts
Polyethylene glycol, 4 parts of enzyme activities are 10000U/g lipase, 4 parts of ethyl ethane sulphonates, 4 parts of alginic acids, 0.5 part of maleic hydrazide, 1
Part Tween-60,0.15 part of gum arabic, 12 parts of water.
The preparation method be the same as Example 1 of wheat cold-resistant derivant.
The application process of wheat cold-resistant derivant is as follows：
Step 1, every mu weighs 40g wheat cold-resistant derivants, is diluted with water standby after 800 times；
Step 2, in 4 days before low temperature arriving, wheat is sprayed with the wheat cold-resistant derivant after being diluted in step 1
Stem and wheat leaf blade.
Embodiment 3
A kind of wheat cold-resistant derivant, its raw material is made up of the component of following parts by weight：15 parts of amino-oligosaccharides, 5 parts
Polyethylene glycol, 3 parts of enzyme activities are 10000U/g lipase, 5 parts of ethyl ethane sulphonates, 3 parts of alginic acids, 1 part of maleic hydrazide, 2 parts
Arlacel-80,0.1 part of soybean protein glue, 10 parts of water.
The preparation method be the same as Example 1 of wheat cold-resistant derivant；
The application process of wheat cold-resistant derivant is as follows：
Step 1, every mu weighs 50g wheat cold-resistant derivants, is diluted with water standby after 1000 times；
Step 2, in 5 days before low temperature arriving, wheat is sprayed with the wheat cold-resistant derivant after being diluted in step 1
Stem and wheat leaf blade.
It should be noted that low temperature refers to environment temperature≤0 DEG C.
Embodiment 1-3 has prepared the wheat cold-resistant derivant worked well, the wheat prepared using embodiment 1-3
Cold-resistant derivant is in the peaceful town pilot in Xinxiang City, Henan Province Yuanyang County, and research wheat cold-resistant derivant is to wheat leaf blade physical signs
Influence, specific experiment process and experimental result are as follows.
(1) influence of the wheat cold-resistant derivant to wheat seedlings blade indices
Wheat seed is sterilized, soaked, vernalization, treat that seed just shows money or valuables one carries unintentionally, select full, uniform wheat seed and sow
Into culture dish, per 30, ware, cultivated with Hoagland (Gram suddenly) nutrient solution in illumination box, condition of culture is
25/20 DEG C (day night), 300 μm of ol/m of intensity of illumination2/ s, periodicity of illumination
14/l0h (day night), relative humidity is 65 ± 5%；When wheat seedling growth to 21 heart stage of leaf, embodiment 1- is used
Obtained wheat cold-resistant derivant is sprayed to wheat seedlings blade in 3, blade is moistened completely, but liquid is not flowed down, cloudy
Property control sample spray with the clear water of equivalent；Wheat seedling is placed in 0 DEG C of low temperature stress 3d after 48h, wheat seedlings blade is then determined
Middle MDA, soluble sugar, soluble protein, the content of chlorophyll and proline, concrete outcome are shown in Table 1.
It should be noted that in this experiment, mda content is determined using thiobarbituricacidα- method, soluble sugar content is adopted
Determined with anthrone colorimetry, soluble protein content is determined using Coomassie brilliant G-250 method, chlorophyll content uses light splitting light
Degree method is determined, and proline content is determined using acid ninhydrine method.
Influence of the wheat cold-resistant derivant of table 1 to wheat seedlings blade indices
As it can be seen from table 1 compared to control sample, contained MDA (MDA) in embodiment 1-3 wheat leaf blade, can
Dissolubility sugar, soluble protein, chlorophyll and the notable of proline are raised.MDA is the product of Lipid peroxidation metabolism under salt stress,
The big I of its content represents the size of membrane damage degree, and embodiment 1-3 wheat seedling is under low temperature stress, MDA in leaves
Content is significantly raised, and injury of the low temperature stress to cell membrane can be relieved after being handled through cold-resistant derivant；Low temperature stress bar
Under part, wheat seedlings blade soluble sugar, soluble protein and proline content are substantially reduced, energy after being handled through cold-resistant derivant
Three's content is enough improved, plant overall metabolic level is improved, and maintain plant osmosis regulating power；Cold-resistant derivant can also
Wheat seedlings blade chlorophyll content is improved, to promote photosynthesis, it is ensured that plant normal growth is developed.
(2) influence of the wheat cold-resistant derivant to wheat seedling biomass
Embodiment 1-3 and the wheat seedling of negative control example move under normal temperature condition after low temperature stress 3d and cultivate 7d,
Its height of seedling, weight in wet base are measured again, and then with, to constant weight, then dry weight is determined after 105 DEG C of oven for drying 2h, specific experiment the results are shown in Table 2：
Influence of the wheat cold-resistant derivant of table 2 to wheat seedling biomass
| Group | Height of seedling (cm) | Seedling weight in wet base (g) | Seedling stem weight (g) |
| Control sample | 23.58 | 0.36 | 0.048 |
| Embodiment 1 | 26.92 | 0.56 | 0.068 |
| Embodiment 2 | 27.11 | 0.51 | 0.072 |
| Embodiment 3 | 26.99 | 0.58 | 0.066 |
From table 2 it can be seen that after being handled through cold-resistant derivant, height of seedling, the seedling weight of wheat seedling are significantly increased, explanation
Embodiment 1-3 wheat cold-resistant derivant can improve the ability that wheat seedling resists low temperature stress, to ensure its normal growth.
(3) influence of the wheat cold-resistant derivant to wheat yield
Embodiment 1-3 and the wheat seedling of negative control example move under normal temperature condition after low temperature stress 3d and cultivate 7d,
Then wheat seedling is moved into field growing again, and be grown in the field period, before low temperature arriving, to embodiment 1-3
Group wheat the corresponding time and method according to embodiment 1-3 sprays wheat cold-resistant derivant respectively, treat wheat growth into
After the ripe phase, each group wheat single-strain tassel number, grain number per spike, mass of 1000 kernel and every group of yield are investigated respectively, and concrete outcome is shown in Table 3：
Influence of the wheat cold-resistant derivant of table 3 to wheat yield
From table 3 it can be seen that do not spray the single-strain tassel number of the control sample of cold-resistant derivant low-temperature treatment, grain number per spike, thousand
Weight and yield wheat cryogenically treated compared with cold-resistant derivant are low, it is seen then that cold-resistant derivant of the invention mainly passes through increase
Mature spike number and grain number per spike increase the yield of wheat.
It should be noted that when being related to number range in claims of the present invention, it is thus understood that each number range
Any one numerical value can select between two end points and two end points, due to step method and the embodiment 1-3 phases of use
Together, in order to prevent from repeating, description of the invention preferred embodiment, but those skilled in the art once know substantially
Creative concept, then can make other change and modification to these embodiments.So, appended claims are intended to be construed to bag
Include preferred embodiment and fall into having altered and changing for the scope of the invention.
Obviously, those skilled in the art can carry out the essence of various changes and modification without departing from the present invention to the present invention
God and scope.So, if these modifications and variations of the present invention belong to the scope of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to comprising including these changes and modification.
Claims (8)
1. a kind of wheat cold-resistant derivant, it is characterised in that its raw material is made up of the component of following parts by weight：
10-15 parts of amino-oligosaccharide, 5-10 parts of polyethylene glycol, 3-5 parts of lipase, 3-5 parts of ethyl ethane sulphonate, alginic acid 3-5
Part, 0.5-1 parts of maleic hydrazide, 1-2 parts of emulsifying agent, 0.1-0.2 parts of thickener, 10-15 parts of water.
2. wheat cold-resistant derivant according to claim 1, it is characterised in that its raw material by following parts by weight component
Composition：12 parts of amino-oligosaccharide, 8 parts of polyethylene glycol, 4 parts of lipase, 4 parts of ethyl ethane sulphonate, 4 parts of alginic acid, maleic hydrazide 0.5
Part, 1 part of emulsifying agent, 0.15 part of thickener, 12 parts of water.
3. wheat cold-resistant derivant according to claim 1, it is characterised in that the lipase activity is 10000U/g.
4. wheat cold-resistant derivant according to claim 1, it is characterised in that the surfactant is Tween-40, told
One or more in temperature -60, Arlacel-60, Arlacel-80.
5. wheat cold-resistant derivant according to claim 1, it is characterised in that the thickener is sodium alginate, cheese
One or more in element, guar gum, chitosan, gum arabic, xanthans, soybean protein glue.
6. a kind of application process of the wheat cold-resistant derivant described in any one of claim 1-5.
7. the application process of wheat cold-resistant derivant according to claim 6, it is characterised in that specifically according to following steps
Implement：
Step 1, every mu weighs 30-50g wheat cold-resistant derivants, is diluted with water standby after 500-1000 times；
Step 2, in 3-5 days before low temperature arriving, wheat stalk is sprayed with the wheat cold-resistant derivant after being diluted in step 1
And wheat leaf blade.
8. the application process of wheat cold-resistant derivant according to claim 7, it is characterised in that the low temperature refers to environment
Temperature≤0 DEG C.
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| CN110973155A (en) * | 2019-12-19 | 2020-04-10 | 青岛农业大学 | Cold-proof agent for wheat late spring coldness |
| CN111011394A (en) * | 2019-12-12 | 2020-04-17 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
| CN111543444A (en) * | 2020-04-01 | 2020-08-18 | 威海文隆梦阳农业发展有限公司 | Chinese medicine biological preparation plant antifreezing solution and preparation method thereof |
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| CN110973155A (en) * | 2019-12-19 | 2020-04-10 | 青岛农业大学 | Cold-proof agent for wheat late spring coldness |
| CN111543444A (en) * | 2020-04-01 | 2020-08-18 | 威海文隆梦阳农业发展有限公司 | Chinese medicine biological preparation plant antifreezing solution and preparation method thereof |
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