CN105494424A - Wheat cold-resistant inducer and application method thereof - Google Patents
Wheat cold-resistant inducer and application method thereof Download PDFInfo
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- CN105494424A CN105494424A CN201510997562.2A CN201510997562A CN105494424A CN 105494424 A CN105494424 A CN 105494424A CN 201510997562 A CN201510997562 A CN 201510997562A CN 105494424 A CN105494424 A CN 105494424A
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- wheat
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- resistant
- salicylic acid
- resistant derivant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
Abstract
The invention discloses wheat cold-resistant inducer and an application method thereof. The wheat cold-resistant inducer is a mixed solution prepared from hydrogen peroxide, salicylic acid, tween and water. Each liter of wheat cold-resistant inducer is prepared from 80-200 mg of hydrogen peroxide, 200-350 mg of salicylic acid, 80-150 mg of tween and the balance water. The application method of the wheat cold-resistant inducer includes the steps of spraying the wheat cold-resistant inducer on leaves of wheat in 12-24 h before the low temperature comes, wherein 25-30 kg of inducer is applied per mu, and the low temperature is the environment temperature lower than or equal to 0 DEG C. The wheat cold-resistant inducer is good in low-temperature-resistant effect, the low-temperature stress resistance of wheat can be enhanced, damage caused by the low temperature to growth of wheat is reduced, the yield of wheat is increased, the inducer is safe, environmentally friendly, free of residues, free of phytotoxicity to wheat growth and free of pollution to the environment.
Description
Technical field
The invention belongs to wheat cultivation technical field, be specifically related to the cold-resistant derivant of a grow wheat and application process thereof.
Background technology
Winter wheat is China's staple food crop, its breeding time is grown, experience spring and summer four seasons autumn and winter, its shooting stage of turning green is vulnerable to the impact that the Low Temperature in Spring such as cold in the late spring, late frost is coerced, Low Temperature in Spring harm has become the Major Natural Disasters of China's Wheat Production, have a strong impact on wheat yield, threaten grain security.In recent decades, the probability of happening of China's Frost Damage of Winter Wheat is obvious ascendant trend, become one of major obstacle factor of some area restriction Wheat Production, therefore research is defendd the low temperature freezing-disaster Hazard Technologies such as frost damage, is improved cold tolerance, significant to guarantee improving yield of wheat stable yields.Publishedly at present be applied to cold-resistant conditioning agent in Wheat Production (if number of patent application is 201410290669.9,201410290668.4 etc.) complicated component, preparation method is loaded down with trivial details, production cost is high.The application have studied the good cold-resistant derivant of wheat of simple, with low cost, the anti-cryogenic effect of a kind of composition, and the application of this product has important economic implications for the production development of wheat.
Hydrogen peroxide (also known as hydrogen peroxide, Hydrogenperoxide, H
2o
2) be a kind of active oxygen produced in biological cell metabolic process, in plant corpus, SCAVENGING SYSTEM OF ACTIVATED OXYGEN can make its content remain under injury level under normal circumstances, but under environment stress, the generation of active oxygen can exceed System Cleaning ability, causes oxidative damage thus.A very long time in the past, people are by H
2o
2be considered as a kind of harmful products of cellular metabolism, but more and more study proof, plant can utilize H
2o
2and amplified as signaling molecule, and then regulation and control growth and development process and the response to various biology and abiotic stimulation.Various biology and abiotic stress all can on certain level H in inducing cell
2o
2generation, and then regulate and control the signal transduction of a series of stress response.Research shows, hydrogen peroxide, salicylic acid etc. participate in the adjustment of many abiotic stresses, improve the resistance of multiple abiotic stress.For reducing the harm of low temperature cold damage, improving wheat yield, using the cold-resistant derivant of certain material, by being conducive to the advantage increasing resistance of wheat derivant, strengthening resistance of wheat.
Summary of the invention
The object of this invention is to provide the cold-resistant derivant of wheat that simple, with low cost, the anti-cryogenic effect of a kind of composition is good, the cold-resistant derivant of this wheat can strengthen the cold resistance of wheat, improves wheat anti-seismic design, reduces chilling damage, reduce low temperature loss, improve wheat yield.
The technical solution used in the present invention is:
The cold-resistant derivant of one grow wheat, it is characterized in that, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 80mg-200mg, salicylic acid 200mg-350mg, Tween 80 mg-150mg, and surplus is water.
According to the cold-resistant derivant of above-mentioned wheat, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 100mg, salicylic acid 300mg, tween 100mg, and surplus is water.
The application process of the cold-resistant derivant of above-mentioned wheat is: before low temperature arrives in 12-24h, spray the blade of wheat with the cold-resistant derivant of wheat, the amount of application of every mu is 25-30kg, and wherein, described low temperature refers to that environmental temperature is less than or equal to 0 DEG C.
The beneficial effect that the present invention obtains:
(1) the anti-cryogenic effect of the cold-resistant derivant of wheat of the present invention is good, the cold resistance of wheat can be strengthened, improve the anti-low temperature stress ability of wheat, reduce low temperature to the harm of wheat growth, reduce low temperature loss, improve wheat yield, meanwhile, the cold-resistant derivant of wheat of the present invention also has booster action to the drought resistance improving wheat.
(2) the cold-resistant derivant composition of wheat of the present invention is simple, with low cost, uses conveniently, can produce on a large scale and apply, have important economic implications.
(3) the cold-resistant derivant safety and environmental protection of wheat of the present invention, noresidue, and also to wheat growth without poisoning, safer to people and animals, environmentally safe.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but do not limit the scope of the invention.
Embodiment 1:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 100mg, salicylic acid 300mg, tween 100mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 300mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 100mg hydrogen peroxide, 100mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is: before low temperature arrives in 12-24h, spray the blade of wheat with the cold-resistant derivant of wheat, the amount of application of every mu is 25kg, and wherein, described low temperature refers to that environmental temperature is less than or equal to 0 DEG C.
Embodiment 2:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 80mg, salicylic acid 350mg, tween 100mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 350mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 80mg hydrogen peroxide, 100mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is: before low temperature arrives in 12-24h, spray the blade of wheat with the cold-resistant derivant of wheat, the amount of application of every mu is 30kg, and wherein, described low temperature refers to that environmental temperature is less than or equal to 0 DEG C.
Embodiment 3:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 120mg, salicylic acid 250mg, tween 120mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 250mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 120mg hydrogen peroxide, 120mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is: before low temperature arrives in 12-24h, spray the blade of wheat with the cold-resistant derivant of wheat, the amount of application of every mu is 28kg, and wherein, described low temperature refers to that environmental temperature is less than or equal to 0 DEG C.
Embodiment 4:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 150mg, salicylic acid 200mg, Tween 80 mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 200mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 150mg hydrogen peroxide, 80mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is: before low temperature arrives in 12-24h, spray the blade of wheat with the cold-resistant derivant of wheat, the amount of application of every mu is 26kg, and wherein, described low temperature refers to that environmental temperature is less than or equal to 0 DEG C.
Embodiment 5:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 180mg, salicylic acid 280mg, tween 150mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 280mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 180mg hydrogen peroxide, 150mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is: before low temperature arrives in 12-24h, spray the blade of wheat with the cold-resistant derivant of wheat, the amount of application of every mu is 29kg, and wherein, described low temperature refers to that environmental temperature is less than or equal to 0 DEG C.
Embodiment 6:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 200mg, salicylic acid 200mg, tween 140mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 200mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 200mg hydrogen peroxide, 140mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is identical with embodiment 1.
Embodiment 7:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 120mg, salicylic acid 300mg, tween 120mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 300mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 120mg hydrogen peroxide, 120mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is identical with embodiment 2.
Embodiment 8:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 160mg, salicylic acid 220mg, tween 90mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 220mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 160mg hydrogen peroxide, 90mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is identical with embodiment 3.
Embodiment 9:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 100mg, salicylic acid 340mg, tween 130mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 340mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 100mg hydrogen peroxide, 130mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is identical with embodiment 3.
Embodiment 10:
The cold-resistant derivant of one grow wheat, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 150mg, salicylic acid 320mg, tween 100mg, and surplus is water.
The preparation method of the cold-resistant derivant of described wheat is: take 320mg salicylic acid and be dissolved in 500ml water and prepare salicylic acid solution, 150mg hydrogen peroxide, 100mg tween is added in salicylic acid solution, be uniformly mixed, then be settled to 1L with water, after stirring, both obtain the cold-resistant derivant of wheat.
The application process of the cold-resistant derivant of described wheat is identical with embodiment 1.
Embodiment 11:
The present embodiment uses cold-resistant derivant obtained in embodiment of the present invention 1-10 to carry out sprinkling process to the wheat being in the shooting stage, and the cold-resistant derivant of research wheat is on the impact of wheat leaf blade physical signs.
Specific experiment is: choose that the condition of culture being in the shooting stage is consistent, growing way uniform field pipe plants wheat 60 and manage (pipe diameter is 20cm, pipe range 60cm, often pipe 10 strain wheat), be divided into 12 groups (often organizing 5 pipes); Respectively to the cold-resistant derivant of wheat that the pipe cultivation wheat leaf blade sprinkling embodiment of the present invention 1-10 of 1-10 group prepares, plant wheat leaf blade to the pipe of the 11st, 12 group and spray clear water, wherein, the standard of sprinkling is: make blade completely moistening, but liquid does not flow down; After 12h, the pipe of 1-11 group is planted wheat immigration phytotron and carry out low temperature induction process, after low temperature induction process terminates, 1-11 group pipe is planted wheat and again move to field growing.Still field growing (recording two days maximum air temperatures is 23 DEG C, and the minimum air temperature is 5 DEG C, mean temperature of air 12.7 DEG C) after 12nd group of pipe cultivation wheat leaf blade sprayed clear water.1-11 group pipe is planted wheat and is moved to field growing after 12 hours, that gets 1-12 group wheat respectively goes up expanded leaves most as research object, wheat is planted for contrast with the 12nd group of pipe, measure 1-12 group pipe respectively and plant the content that wheat goes up malonaldehyde, soluble sugar, soluble protein and proline in expanded leaves blade most, it the results are shown in Table 1.
Wherein, the process of described low temperature induction process is: with the speed of 2 DEG C/h, the temperature of phytotron is reduced to 0 DEG C, and temperature is maintained 0 DEG C of 48h, then with the speed of 2 DEG C/h, the temperature of phytotron is elevated to outdoor temperature.
In this experiment, mda content adopts thiobarbituricacidα-method side to measure, and soluble sugar content adopts anthrone colorimetry to measure, and soluble protein content adopts Coomassie brilliant G-250 method to measure, and proline content adopts acid ninhydrine method to measure.
Malonaldehyde is one of product of Lipid peroxidation metabolism, the size of the large I reaction film degree of injury of its content, and therefore in plant senescence physiology and Resistance Physiology research, the content of malonaldehyde is a common counter.As shown in Table 1, in this experiment, in the 11st group of wheat leaf blade, the content of malonaldehyde significantly raises, and the content spraying malonaldehyde in the 1-10 group wheat leaf blade of cold-resistant derivant differs less with the 12nd group of wheat, this shows that spraying the cold-resistant derivant of the present invention obviously can alleviate the injury of low temperature stress to wheat leaf blade cell membrane.
Soluble sugar, soluble protein and proline are the osmotic adjustments of Chinese medicine in plant cell, to the total metabolism level, the maintenance plant osmosis regulating power that improve plant, there is important effect, under low temperature stress condition, in this experiment, in the 11st group of wheat leaf blade, the content of soluble sugar, soluble protein and proline is lower, differs larger with the 12nd group; And the content spraying soluble sugar, soluble protein and proline in the 1-10 group wheat leaf blade of cold-resistant derivant is higher, differ less with the 12nd group of wheat, it can be said that the cold-resistant derivant of the bright the present invention of spraying can improve the content of soluble sugar in wheat leaf blade, soluble protein and proline, metaboilic level and the osmotic adjustment ability of wheat can be improved to a certain extent, thus improve the impact self resisting extraneous low temperature.
The cold-resistant conditioning agent of table 1 wheat is on the impact of wheat seedlings blade indices
Embodiment 12: cold-resistant derivant is on the impact of wheat yield
The present embodiment uses cold-resistant derivant obtained in embodiment of the present invention 1-10 to carry out sprinkling process to the wheat being in the shooting stage, and the cold-resistant derivant of research wheat is on the impact of wheat yield.
Specific experiment is: choose that the condition of culture being in the shooting stage is consistent, growing way uniform field pipe plants wheat 60 and manage (pipe diameter is 20cm, pipe range 60cm, often pipe 10 strain wheat), be divided into 12 groups (often organizing 5 pipes); Respectively to the cold-resistant derivant of wheat that the pipe cultivation wheat leaf blade sprinkling embodiment of the present invention 1-10 of 1-10 group prepares, plant wheat leaf blade to the pipe of the 11st, 12 group and spray clear water, wherein, the standard of sprinkling is: make blade completely moistening, but liquid does not flow down; After 12h, the pipe of 1-11 group is planted wheat immigration phytotron and carry out low temperature induction process, after low temperature induction process terminates, 1-11 group pipe is planted wheat and again move to field growing.12nd group of pipe was planted after wheat leaf blade sprayed clear water and is placed in field growing always.After 1-12 group wheat growth to maturing stage, investigate the output that 1-12 group pipe plants wheat single-strain tassel number, grain number per spike, thousand kernel weight and every pipe wheat respectively, with the 12nd group for contrast, add up different cold-resistant derivant to wheat yield and constituent element impact thereof, it the results are shown in Table 2.
Wherein, the process of described low temperature induction process is: with the speed of 2 DEG C/h, the temperature of phytotron is reduced to 0 DEG C, and temperature is maintained 0 DEG C of 48h, then with the speed of 2 DEG C/h, the temperature of phytotron is elevated to outdoor temperature.
As shown in Table 2, the 11st group of pipe not spraying cold-resistant derivant low temperature treatment plant the single-strain tassel number of wheat, grain number per spike, thousand kernel weight and every pipe output comparatively low temperature treatment the 12nd group reduce by 1.2,14.8,8.9g and 31.3g respectively, and the 1-10 group of spraying cold-resistant derivant is compared with the 12nd group, only reduce by 0.4,5.2,5.3g and 15.1g.The 1-10 group of spraying cold-resistant derivant, compared with the 11st group, has certain protective effect, on average increases by 0.8,9.7,4.4g and 16.2g respectively.Visible, the cold-resistant derivant of the present invention increases the output of wheat mainly through increase mature spike number and grain number per spike.
The cold-resistant derivant of table 2 wheat is on the impact of wheat yield
Claims (3)
1. the cold-resistant derivant of a grow wheat, it is characterized in that, the mixed solution that the cold-resistant derivant of described wheat is made up of hydrogen peroxide, salicylic acid, tween and water, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 80mg-200mg, salicylic acid 200mg-350mg, Tween 80 mg-150mg, and surplus is water.
2. the cold-resistant derivant of wheat according to claim 1, is characterized in that, in often liter of cold-resistant derivant of wheat, the content of each component is: hydrogen peroxide 100mg, salicylic acid 300mg, tween 100mg, and surplus is water.
3. an application process for the cold-resistant derivant of wheat according to claim 1, is characterized in that, before low temperature arrives in 12-24h, the blade of wheat is sprayed with the cold-resistant derivant of wheat, the amount of application of every mu is 25-30kg, and wherein, described low temperature refers to that environmental temperature is less than or equal to 0 DEG C.
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| CN107232200A (en) * | 2017-05-12 | 2017-10-10 | 河南农业大学 | Defend the composite regulator and method of wheat Spring cold injury |
| CN107242250A (en) * | 2017-06-16 | 2017-10-13 | 淮北师范大学 | A kind of wheat cold-resistant derivant and its application process |
| CN111011394A (en) * | 2019-12-12 | 2020-04-17 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107232200A (en) * | 2017-05-12 | 2017-10-10 | 河南农业大学 | Defend the composite regulator and method of wheat Spring cold injury |
| CN107242250A (en) * | 2017-06-16 | 2017-10-13 | 淮北师范大学 | A kind of wheat cold-resistant derivant and its application process |
| CN111011394A (en) * | 2019-12-12 | 2020-04-17 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
| CN111011394B (en) * | 2019-12-12 | 2021-02-02 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
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